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1.
John R. Smeda Anthony L. Schilmiller Andre Kessler Martha A. Mutschler 《Molecular breeding : new strategies in plant improvement》2017,37(8):104
The tomato breeding line, CU071026, was bred to accumulate high levels of the insect control compounds called acylsugars, which are exuded from glandular trichomes. The acylsugars of CU071026 exhibit a characteristic profile of acylsugar composition and constituent fatty acid acyl groups that is distinct from that of the progenitor wild tomato, Solanum pennellii LA716. A prior study reported the transfer of three QTL (FA2, FA7, and FA8), from S. pennellii LA716, that are associated with changes in acylsugar chemistry into CU071026 and demonstrated that the resulting lines, each of which possesses one of these QTL, displayed a unique acylsugar and fatty acid profile distinct from that characteristic of the acylsugars of CU071026 and each other. The current study utilized marker-assisted backcrossing to combine pairs of two of these QTL or all three of these QTL. This created a new set of lines, which allowed evaluation of the combinatory effects of FA2QTL, FA7QTL, and FA8QTL, on acylsugar level and acylsugar and fatty acid profile and diversity. Analysis of the new tomato lines revealed that these QTL interacted additively and epistatically to alter acylsugar level and chemistry, increasing the diversity of fatty acid constituents and/or acylsugar chemotypes present in the exudates of some of the lines. Extensive characterization of the lines for acylsugar level, through a spectrophotometric invertase assay, and acylsugar chemistry, through gas and liquid chromatography-mass spectrometry, allowed association of the QTL interactions with aspects of acylsugar chemotype. The evaluated fatty acids and acylsugars accumulated by the set of lines generally displayed high heritability and minimal environmental effect, which is discussed. The QTL interactions that govern a more diverse acylsugar and fatty acid profile provide valuable information for the generation of tomato lines with improved acylsugar efficacy against pests of tomato. 相似文献
2.
Brian M. Leckie Rayko Halitschke Darlene M. De Jong John R. Smeda André Kessler Martha A. Mutschler 《Molecular breeding : new strategies in plant improvement》2014,34(3):1201-1213
Acylsugars are secondary metabolites with proven insect resistance properties that are produced by many Solanaceous species including Solanum pennellii, a wild relative of tomato. The acylsugar chemotypes of S. pennellii varies greatly within its natural range and might be the product of plant/insect coevolution. The S. pennellii accession LA716 was used to transfer increased levels of acylsugar production into the cultivated tomato, resulting in the acylsugar tomato breeding line CU071026. S. pennellii accession LA716 produces high levels of acylsugars with chemotypes that differ greatly from those produced by CU071026 or the trace acylsugars of cultivated tomato. Understanding the genetic regulation of acylsugar chemistry will aid efforts to breed acylsugar production into cultivated tomato, allowing for alteration of both acylsugar base moieties and fatty acid profiles. This study uses a BC1F1 population produced from the cross of S. pennellii LA716 and CU071026 with CU071026 as the recurrent parent to identify QTL that change the fatty acid profile of acylsugars. Multiple QTL and epistatic interactions between QTL were detected including three QTL on chromosomes 2, 5, and 7, which significantly alter the percentage of extended iso-odd branched fatty acids and straight chain fatty acids on the acylsugars. We also report the introgression of one of these QTL, FA 2, into CU071026, resulting in a new tomato line with significantly increased i11:0 as a percentage of the fatty acids in its acylsugars. Candidate genes for these QTL and the impacts of altering acylsugar fatty acid are discussed. 相似文献
3.
Brian M. Leckie Darlene M. De Jong Martha A. Mutschler 《Molecular breeding : new strategies in plant improvement》2012,30(4):1621-1634
Solanum pennellii LA716, a wild relative of tomato, produces acylsugars, an insect resistance compound with activity against many tomato insect pests. Breeding of cultivated tomato using S. pennellii LA716 as a donor parent has led to the development of the elite acylsugar-producing tomato breeding line CU071026. CU071026 contains five introgressed S. pennellii genomic regions, and produces acylsugars at moderate levels that are effective against insect pests. A BC1F1 population was created by crossing the F1 CU071026?×?S. pennellii LA716 with CU071026 as the recurrent parent; this BC1F1 population was used to identify additional regions of the S. pennellii genome important for further improvement of acylsugar production. This population was genotyped with 94 markers in the segregating regions and phenotyped for level of acylsugar production. Using QTLNetwork 2.1 for the detection of quantitative trait loci (QTL) and epistatic interactions, this study identified five QTL for total acylsugar level. Additionally, two epistatic interactions between QTL were found to control significant levels of total acylsugar production. Two of the QTL identified were further evaluated in silverleaf whitefly (Bemisia tabaci) field cage trials using acylsugar breeding lines that differ for the presence/absence of these QTL. While high levels of silverleaf whitefly resistance were observed in all acylsugar breeding lines, lines containing the additional QTL on either chromosomes 6 or 10 had increased levels of total acylsugar production and reduced incidence of whitefly. Acylsugar lines containing the chromosome 6 QTL also had increased density of the type IV glandular trichomes which produce and exude acylsugars. 相似文献
4.
John R. Smeda Anthony L. Schilmiller Taylor Anderson Sulley Ben-Mahmoud Diane E. Ullman Thomas M. Chappell Andre Kessler Martha A. Mutschler 《Molecular breeding : new strategies in plant improvement》2018,38(1):3
Acylsugars are important insect defense compounds produced at high levels by glandular trichomes of the wild tomato, Solanum pennellii. Marker-assisted selection was used to select for plants containing the three AGQTL named AG3QTL, AG4QTL, and AG11QTL from self-pollinated populations derived from an interspecific backcross population of CU071026 x (CU071026 x S. pennellii LA716). High acylglucose-accumulating lines were selected from these populations that possess these three AGQTL and the fewest number of extraneous S. pennellii LA716 introgressions. Incorporation of these three acylglucose QTL in the presence of the five standard S. pennellii introgressions of CU071026 altered acylsugar level and sugar moiety, demonstrating epistatic interactions between the acylglucose QTL on both of these traits. Comparison of the lines generated from the two breeding techniques indicated the three acylglucose QTL are essential but not necessarily sufficient for the production of elevated levels of acylglucose acylsugars. Fine-mapping of AG3QTL, AG4QTL, and AG11QTL resulted in less than 1 Mbp intervals for the locations of AG4QTL and AG11QTL; proposals of the causal genes underlying these acylglucose QTL are discussed. Characterization of the fatty acid profile of lines selected out of the interspecific backcross populations revealed an increase in the proportion of acylsugar n-C10 fatty acid acyl chains, possibly governed by one or more of the three acylglucose QTL. Characterization of the acylsugar profile of acylglucose lines selected from the interspecific backcross populations also demonstrated interactions among the acylglucose QTL to further modulate the diversity of acylsugars accumulated. Evaluation of an acylglucose line and controls against the tomato insect pest Frankliniella occidentalis demonstrated that levels of resistance differed among these lines and that the acylsugars accumulated by the acylglucose line were effective at reducing both F. occidentalis oviposition and incidence of Tomato spotted wilt orthotospovirus. However, of some of the acylglucose lines and hybrids tested against Spodoptera exigua did not indicate differences for larval weight gain and survival. 相似文献
5.
Brian M. Leckie Darlene M. De Jong Martha A. Mutschler 《Molecular breeding : new strategies in plant improvement》2013,31(4):957-970
Acylsugars are broad-spectrum insect resistance sugar esters produced at very high levels by some accessions of the wild tomato, Solanum pennellii. Transferring acylsugar production from S. pennellii LA716 to cultivated tomato through traditional breeding developed the benchmark acylsugar breeding line CU071026. The base moiety of acylsugars (sucrose vs. glucose) can vary among S. pennellii accessions. Additionally the accession S. pennellii LA716 produces almost exclusively acylglucoses, but the breeding line CU071026 derived from S. pennellii LA716 produces exclusively acylsucroses. This study uses a BC1F1 and a BC1F2 population derived from the cross CU071026 × (CU071026 × S. pennellii LA716) to identify and confirm the action of three quantitative trait loci (QTL) on chromosomes 3, 4, and 11. The QTL on chromosomes 3 and 11 are both required for acylglucose production, while addition of the chromosome 4 QTL affects the level of acylglucose produced in the presence of the QTL on chromosomes 3 and 11. A three-way interaction between these acylglucose QTL was confirmed with a post hoc ANOVA. Identification of these three QTL provides a blueprint for breeding to shift acylsucrose production to acylglucose production in tomato breeding lines. The implications of these QTL and two additional QTL affecting total acylsugar level in the BC1F2 are discussed. 相似文献
6.
Lawson Darlene M. Lunde China F. Mutschler Martha A. 《Molecular breeding : new strategies in plant improvement》1997,3(4):307-317
Acylsugars exuded from type IV trichomes mediate the multiple pest resistance found in the wild tomato species, Lycopersicon pennellii. A marker-assisted selection breeding program was used to attempt the transfer of the ability to accumulate acylsugars to cultivated tomato. RFLP and PCR-based markers were used through three backcross generations to select plants containing 5 target regions associated by QTL analysis with acylsugar accumulation. The BC1F1 plant selected possessed all 5 target regions and accumulated acylsugars at a moderate level similar to that of the interspecific F1 control. The BC2F1 and BC3F1 selections contained complementary subsets of the 5 target regions and did not accumulate acylsugars. BC3F1 plants with complementary subsets of the 5 target regions were intermated to produce populations segregating for the 5 target regions. From 1000 BC3F1-intermated plants, three plants were found which accumulated acylsugars at low levels and contained 3 to 5 of the target regions. The recovery of acylsugar accumulation in progeny of the intermated BC3F1 plants supports the involvement of at least some of the 5 target regions in acylsugar biosynthesis. However, since the levels of acylsugars accumulated by these plants were lower than that of the interspecific F1, it is likely that another, as of yet unidentified, region is necessary for accumulation of higher levels of acylsugars. 相似文献
7.
Brian M. Leckie Damon A. D'Ambrosio Thomas M. Chappell Rayko Halitschke Darlene M. De Jong André Kessler George G. Kennedy Martha A. Mutschler 《PloS one》2016,11(4)
Acylsugars are secondary metabolites exuded from type IV glandular trichomes that provide broad-spectrum insect suppression for Solanum pennellii Correll, a wild relative of cultivated tomato. Acylsugars produced by different S. pennellii accessions vary by sugar moieties (glucose or sucrose) and fatty acid side chains (lengths and branching patterns). Our objective was to determine which acylsugar compositions more effectively suppressed oviposition of the whitefly Bemisia tabaci (Gennadius) (Middle East—Asia Minor 1 Group), tobacco thrips, Frankliniella fusca (Hinds), and western flower thrips, Frankliniella occidentalis (Pergande). We extracted and characterized acylsugars from four S. pennellii accessions with different compositions, as well as from an acylsugar-producing tomato breeding line. We also fractionated the acylsugars of one S. pennellii accession to examine the effects of its components. Effects of acylsugars on oviposition were evaluated by administering a range of doses to oviposition sites of adult whiteflies and thrips in non-choice and choice bioassays, respectively. The acylsugars from S. pennellii accessions and the tomato breeding line demonstrated differential functionality in their ability to alter the distribution of whitefly oviposition and suppress oviposition on acylsugar treated substrates. Tobacco thrips were sensitive to all compositions while western flower thrips and whiteflies were more sensitive to acylsugars from a subset of S. pennellii accessions. It follows that acylsugars could thus mediate plant-enemy interactions in such a way as to affect evolution of host specialization, resistance specificity, and potentially host differentiation or local adaptation. The acylsugars from S. pennellii LA1376 were separated by polarity into two fractions that differed sharply for their sugar moieties and fatty acid side chains. These fractions had different efficacies, with neither having activity approaching that of the original exudate. When these two fractions were recombined, the effect on both whiteflies and thrips exceeded the sum of the two fractions’ effects, and was similar to that of the original exudate. These results suggest that increasing diversity of components within a mixture may increase suppression through synergistic interactions. This study demonstrates the potential for composition-specific deployment of acylsugars for herbivore oviposition suppression, either through in planta production by tomato lines, or as biocides applied by a foliar spray. 相似文献
8.
Resende JT Maluf WR Cardoso Md Nelson DL Faria MV 《Genetics and molecular research : GMR》2002,1(2):106-116
Acylsugars present in Lycopersicon pennellii are responsible for the high levels of pest resistance often found in this wild tomato taxon. We investigated the inheritance of acylsugar contents in segregating populations of the interspecific tomato cross L. esculentum x L. pennellii and estimated correlations between leaflet acylsugar contents and the levels of mite repellence. Acylsugar contents were quantified with the Sommogy-Nelson colorimetric method in the acessions L. esculentum 'TOM-584' (P(1), low acylsugars), L. pennellii 'LA-716' (P(2), high acylsugars), in the interspecific F(1) (P(1) x P(2)) and in the F(2 )(P(1) x P(2)) generations. Mite resistance was assessed by a repellence test. Broad-sense heritability of acylsugar contents was moderately high (h(2)(b) = 0.476). Frequency distributions in the P(1), P(2), F(1) and F(2) can be explained by the action of a single major locus, with near-complete dominance of the L. esculentum allele for low-acylsugar content over the L. pennellii allele for high content. Indirect selection for high levels of acylsugars in leaflets led to correlated increases in the levels of mite repellency, indicating that acylsugars may be the main factor involved in mite resistance. 相似文献
9.
S. L. Blauth G. A. Churchill M. A. Mutschler 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(3-4):458-467
Lycopersicon pennellii LA716, a wild relative of tomato, is resistant to a number of insect pests due to the accumulation of acylsugars exuded from
type IV trichomes. These acylsugars are a class of compounds including both acylglucoses and acylsucroses. Intraspecific populations
between L. pennellii LA716 and L. pennellii LA1912, the latter an accession that assorts for low-level acylsugar accumulation, were created to study the inheritance
of type IV trichome density, acylsugar accumulation levels, percentage of acylsugars that are acylglucoses, and leaf area.
The F2 population was subsequently used to determine genomic regions associated with these traits. The relative proportion of acylglucoses
and acylsucroses was found to be largely controlled by a single locus near TG549 on chromosome 3. One locus on chromosome
10 showed significant associations with acylsugar levels. In addition, 1 locus on chromosome 4 showed significant associations
with leaf area. Ten additional loci showed modest associations with one or more of the traits examined, 5 of which have been
previously reported.
Received: 13 March 1997 / Accepted: 19 September 1997 相似文献
10.
QTL analysis of pest resistance in the wild tomato Lycopersicon pennellii: QTLs controlling acylsugar level and composition 总被引:2,自引:0,他引:2
M. A. Mutschler R. W. Doerge S. C. Liu J. P. Kuai B. E. Liedl J. A. Shapiro 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(6):709-718
Some accessions of Lycopersicon pennellii, a wild relative of the tomato Lycopersicon esculentum, are resistant to a number of important pests of cultivated tomato due to the accumulation of acylsugars, which constitute 90% of the exudate of type-IV trichomes in L. pennellii LA716. An interspecific F2 population, created by the cross L. esculentum x L. pennellii LA 716, was surveyed for acylsugar accumulation and subjected to RFLP/QTL analysis to determine the genomic regions associated with the accumulation of acylglucoses, acylsucroses, and total acylsugars, as well as with acylglucoses as a percentage of total acylsugars (mole percent acylglucoses). Data were analyzed using MAPMAKER/QTL with and without a log10 transformation. A threshold value of 2.4 (default value for MAPMAKER/QTL) was used, as well as 95% empirically derived threshold values. Five genomic regions, two on chromosome 2 and one each on chromosomes 3, 4 and 11, were detected as being associated with one or more aspects of acylsugar production. The L. esculentum allele is partially dominant to the L. pennellii allele in the regions on chromosomes 2 and 11, but the L. pennellii allele is dominant in the region on chromosome 3. Throughout this study, we report the comparative effects of analytical methodology on the identification of acylsugar QTLs. Similarities between our results and published results for the genus Solanum are also discussed.R. W. Doerge · S.-C. Liu · J. P. Kuai contributed equally to the paper, and we ordered randomly 相似文献
11.
S. L. Blauth J. C. Steffens G. A. Churchill M. A. Mutschler 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):373-381
Acylsugars exuded by type IV glandular trichomes are responsible for insect resistances found in many Lycopersicon pennellii accessions. Acylsugars are complex mixtures composed of polyacylated sugars (glucose or sucrose) esterified to branched and
straight-chain 4 : 0 to 12 : 0 fatty acids. The biogeneses of these unusual fatty acid constituents have their origins in
branched-chain amino acid pathways. However, the mechanism of fatty acid elongation in these systems and the genetic control
of carbon flux from amino acid to fatty acid pathways remain unclear. In this study, we used an intraspecific F2 population derived from the cross between L. pennellii LA716 and L. pennellii LA1912 to examine the genetic basis of acylsugar fatty acid composition. Six QTLs were detected which, combined, explain
23–60% of the variance observed for each of the nine segregating fatty acid constituents. Both correlation data and QTL analysis
data indicate that branched medium-chain fatty acids are synthesized through elongation of short-chain precursors in two-carbon
increments. The proportion of iso-branched acylsugar fatty acids that have an even-carbon chain length was found to be primarily determined by a single locus
that maps to a location 5.5 cM above TG117 on chromosome 8. QTL function in several cases can be inferred from discrete patterns
of fatty acid composition; in other cases, control of acylsugar fatty acid composition appears to be complex.
Received: 7 April 1998 / Accepted: 28 December 1998 相似文献
12.
Anthony L. Schilmiller Gaurav D. Moghe Pengxiang Fan Banibrata Ghosh Jing Ning A. Daniel Jones Robert L. Last 《The Plant cell》2015,27(4):1002-1017
Glandular trichomes from tomato (Solanum lycopersicum) and other species in the Solanaceae produce and secrete a mixture of O-acylsugars (aliphatic esters of sucrose and glucose) that contribute to insect defense. Despite their phylogenetic distribution and diversity, relatively little is known about how these specialized metabolites are synthesized. Mass spectrometric profiling of acylsugars in the S. lycopersicum x Solanum pennellii introgression lines identified a chromosome 11 locus containing a cluster of BAHD acyltransferases with one gene (named Sl-ASAT3) expressed in tip cells of type I trichomes where acylsugars are made. Sl-ASAT3 was shown to encode an acyl-CoA-dependent acyltransferase that catalyzes the transfer of short (four to five carbons) branched acyl chains to the furanose ring of di-acylsucrose acceptors to produce tri-acylsucroses, which can be further acetylated by Sl-ASAT4 (previously Sl-AT2). Among the wild tomatoes, diversity in furanose ring acyl chains on acylsucroses was most striking in Solanum habrochaites. S. habrochaites accessions from Ecuador and northern Peru produced acylsucroses with short (≤C5) or no acyl chains on the furanose ring. Accessions from central and southern Peru had the ability to add short or long (up to C12) acyl chains to the furanose ring. Multiple ASAT3-like sequences were found in most accessions, and their in vitro activities correlated with observed geographical diversity in acylsugar profiles. 相似文献
13.
Tomato fruit ascorbic acid content is linked with monodehydroascorbate reductase activity and tolerance to chilling stress 总被引:1,自引:0,他引:1
Stevens R Page D Gouble B Garchery C Zamir D Causse M 《Plant, cell & environment》2008,31(8):1086-1096
Quantitative trait loci (QTL) mapping is a step towards the identification of factors regulating traits such as fruit ascorbic acid content. A previously identified QTL controlling variations in tomato fruit ascorbic acid has been fine mapped and reveals that the QTL has a polygenic and epistatic architecture. A monodehydroascorbate reductase (MDHAR) allele is a candidate for a proportion of the increase in fruit ascorbic acid content. The MDHAR enzyme is active in different stages of fruit ripening, shows increased activity in the introgression lines containing the wild-type ( Solanum pennellii ) allele, and responds to chilling injury in tomato along with the reduced/oxidized ascorbate ratio. Low temperature storage of different tomato introgression lines with all or part of the QTL for ascorbic acid and with or without the wild MDHAR allele shows that enzyme activity explains 84% of the variation in the reduced ascorbic acid levels of tomato fruit following storage at 4 °C, compared with 38% at harvest under non-stress conditions. A role is indicated for MDHAR in the maintenance of ascorbate levels in fruit under stress conditions. Furthermore, an increased fruit MDHAR activity and a lower oxidation level of the fruit ascorbate pool are correlated with decreased loss of firmness because of chilling injury. 相似文献
14.
Dongwei Xie Yingpeng Han Yuhong Zeng Wei Chang Weili Teng Wenbin Li 《Molecular breeding : new strategies in plant improvement》2012,30(1):169-179
Soybean fatty acids (FAs) are major sources of vegetable oil in the world. The FA composition of soybean is associated with the quality and nutritional value of its oil and food products. The polyunsaturated FAs, particularly linolenic acid (LN), are prone to oxidation by lipoxygenase isozymes and negatively affect the flavor and shelf-life of soybean products. The improvement of FA composition and the increase of oxidative stability has been a major goal of soybean breeding for decades. The objective of the present study was to identify quantitative trait loci (QTL) associated with the low LN and other FA contents in six environments. One hundred and twenty-five recombinant inbred lines (RILs) of F5:7, F5:8 and F5:9 generations derived by the single-seed-descent method from the cross of Hefeng 25 [LN 6.20%; linoleic acid (LI) 53.06%; oleic acid (OL) 19.75%; palmitic acid (PA) 12.16%; stearic acid (ST) 4.97%] and Dongnong L-5 (LN 2.53%; LI 59.30%; OL 24.24%; PA 10.0%; ST 3.99%) were used in this study. A total of 112 simple sequence repeat markers were used to construct a genetic linkage map. Six QTL associated with LN content, four QTL associated with LI content, four QTL associated with OL content, four QTL associated with PA content and one QTL associated with ST content were identified, and mapped onto different linkage groups (LGs). The QTL detected here explained 2.53?C37.30% of phenotypic variation for individual FA in different environments or individual FA means. Of them, the beneficial alleles of QLNB2_1 (close to Satt726), QLNB2_2 (close to Fad3a-4) and QLND1b_1 (close to Satt701), which were associated with low LN content across various environments and LN means, were derived from Dongnong L-5. These three QTL might have great potential value in marker-assisted selection for low LN content in soybean seed. 相似文献
15.
M. García‐Fernández B. Gutiérrez‐Gil E. García‐Gámez J. P. Sánchez J. J. Arranz 《Animal genetics》2010,41(3):324-328
In this work, we analysed 11 genetic markers localized on OAR11 in a commercial population of Spanish Churra sheep to detect QTL that underlie milk fatty acid (FA) composition traits. Following a daughter design, we analysed 799 ewes distributed in 15 half‐sib families. Eight microsatellite markers and three novel SNPs identified in two genes related to fatty acid metabolism, acetyl‐CoA carboxylase α (ACACA) and fatty acid synthase (FASN), were genotyped in the whole population under study. The phenotypic traits considered in the study included 22 measurements related to the FA composition of the milk and three other milk production traits (milk protein percentage, milk fat percentage and milk yield). Across‐family regression analysis revealed four significant QTL at the 5% chromosome‐wise level influencing contents of capric acid (C10:0), lauric acid (C12:0), linoleic conjugated acid (CLA) and polyunsaturated fatty acids (PUFA) respectively. The peaks of the QTL affecting C10:0 and PUFA contents in milk map close to the FASN gene, which has been evaluated as a putative positional candidate for these QTL. The QTL influencing C12:0 content reaches its maximum significance at 58 cM, close to the gene coding for the glucose‐dependent insulinotropic polypeptide. We were not able to find any candidate genes related to fat metabolism at the QTL influencing CLA content, which is located at the proximal end of the chromosome. Further research efforts will be needed to confirm and refine the QTL locations reported here. 相似文献
16.
McNally K.L. Mutschler M.A. 《Molecular breeding : new strategies in plant improvement》1997,3(3):203-212
RAPD primers were identified as giving parent-specific bands when screened with a set of introgression lines containing introgressed regions of Lycopersicon pennellii that encompass 5 quantitative trait loci affiliated with the production and composition of acylsugars, compounds associated with insect resistance. Primers giving L. pennellii introgression specific bands were zonally mapped to identify bands affiliated with the quantitative trait target and flanking regions using subsets of 7 to 16 F2 individuals which contained small overlapping segments (zones) of the L. pennellii genome spanning those regions. Seventeen RAPD primers, agt-related primers, and an agt clone were then used in mapping the complete F2 population of 144 individuals. This work resulted in the identification of RAPD markers for three of the 5 quantitative trait loci and the construction of an integrated RAPD/RFLP genomic map for tomato (Lycopersicon esculentum x L. pennellii LA716) of 111 RAPD and 8 acylglucose transferase related markers added to a framework map of 150 RFLP markers. 相似文献
17.
An 11-bp insertion in Zea mays fatb reduces the palmitic acid content of fatty acids in maize grain 总被引:1,自引:0,他引:1
Li L Li H Li Q Yang X Zheng D Warburton M Chai Y Zhang P Guo Y Yan J Li J 《PloS one》2011,6(9):e24699
The ratio of saturated to unsaturated fatty acids in maize kernels strongly impacts human and livestock health, but is a complex trait that is difficult to select based on phenotype. Map-based cloning of quantitative trait loci (QTL) is a powerful but time-consuming method for the dissection of complex traits. Here, we combine linkage and association analyses to fine map QTL-Pal9, a QTL influencing levels of palmitic acid, an important class of saturated fatty acid. QTL-Pal9 was mapped to a 90-kb region, in which we identified a candidate gene, Zea mays fatb (Zmfatb), which encodes acyl-ACP thioesterase. An 11-bp insertion in the last exon of Zmfatb decreases palmitic acid content and concentration, leading to an optimization of the ratio of saturated to unsaturated fatty acids while having no effect on total oil content. We used three-dimensional structure analysis to explain the functional mechanism of the ZmFATB protein and confirmed the proposed model in vitro and in vivo. We measured the genetic effect of the functional site in 15 different genetic backgrounds and found a maximum change of 4.57 mg/g palmitic acid content, which accounts for ~20-60% of the variation in the ratio of saturated to unsaturated fatty acids. A PCR-based marker for QTL-Pal9 was developed for marker-assisted selection of nutritionally healthier maize lines. The method presented here provides a new, efficient way to clone QTL, and the cloned palmitic acid QTL sheds lights on the genetic mechanism of oil biosynthesis and targeted maize molecular breeding. 相似文献
18.
Elizondo A Araya J Rodrigo R Poniachik J Csendes A Maluenda F Díaz JC Signorini C Sgherri C Comporti M Videla LA 《Obesity (Silver Spring, Md.)》2007,15(1):24-31
Objective: Our aim was to study the fatty acid (FA) composition of liver phospholipids and its relation to that in erythrocyte membranes from patients with obese nonalcoholic fatty liver disease (NAFLD), as an indication of lipid metabolism alterations leading to steatosis. Research Methods and Procedures: Eight control subjects who underwent antireflux surgery and 12 obese patients with NAFLD who underwent subtotal gastrectomy with a gastro‐jejunal anastomosis in Roux‐en‐Y were studied. The oxidative stress status of patients was assessed by serum F2‐isoprostanes levels (gas chromatography/negative ion chemical ionization tandem mass spectrometry). Analysis of FA composition of liver and erythrocyte phospholipids was carried out by gas‐liquid chromatography. Results: Patients with NAFLD showed serum F2‐isoprostanes levels 84% higher than controls. Compared with controls, liver phospholipids from obese patients exhibited significantly 1) lower levels of 20:4n‐6, 22:5n‐3, 22:6n‐3 [docosahexaenoic acid (DHA)], total long‐chain polyunsaturated FA (LCPUFA), and total n‐3 LCPUFA, 2) higher 22:5n‐6 [docosapentaenoic acid (DPAn‐6)] levels and n‐6/n‐3 LCPUFA ratios, and 3) comparable levels of n‐6 LCPUFA. Levels of DHA and DPAn‐6 in liver were positively correlated with those in erythrocytes (r = 0.77 and r = 0.90, respectively; p < 0.0001), whereas DHA and DPAn‐6 showed a negative association in both tissues (r = ?0.79, p < 0.0001 and r = ?0.58, p < 0.01, respectively), associated with lower DHA/DPAn‐6 ratios. Discussion: Obese patients with NAFLD showed marked alterations in the polyunsaturated fatty acid pattern of the liver. These changes are significantly correlated with those found in erythrocytes, thus suggesting that erythrocyte FA composition could be a reliable indicator of derangements in liver lipid metabolism in obese patients. 相似文献
19.
Xiaoxiao Liu Mollie Enright Cornelius S. Barry A. Daniel Jones 《Metabolomics : Official journal of the Metabolomic Society》2017,13(7):85
Introduction
Acylsugar specialized metabolites function as defenses against insect herbivores, and are the most abundant specialized metabolites produced in Solanaceous trichomes. Metabolite profiling provides the foundation for determining the genetic basis of specialized metabolism and its evolution.Objectives
To profile and identify acylsugar specialized metabolites in three Petunia species: P. axillaris, P. integrifolia and P. exserta.Methods
Metabolites were profiled using ultra-high performance liquid chromatography/time-of-flight mass spectrometry (UHPLC/TOF MS). Metabolites were purified using solid phase extraction and HPLC, and structures were established using NMR spectroscopy.Results
Twenty-eight distinct acylsucrose formulas, representing a sampling of more than 100 different detected chemical forms, were purified from three Petunia species and structures have been proposed based on one- and two-dimensional NMR data. 15 of the 28 purified acylsugars were sucrose pentaesters that possess a malonyl group on the fructose ring. These malonate esters can be readily distinguished from other acylsugars based on distinct masses of pseudomolecular ions and fragment ions generated using multiplexed collision-induced dissociation. Chemical diversity of acylsugars was observed between Petunia species, particularly with respect to the lengths of acyl chains and specific acylation positions.Conclusions
These findings suggest substrate selectivity of various acyltransferases in Petunia species.20.
Krämer SD Schütz YB Wunderli-Allenspach H Abbott NJ Begley DJ 《In vitro cellular & developmental biology. Animal》2002,38(10):566-571
Lipids of brain tissue and brain microvascular endothelial cells contain high proportions of long-chain polyunsaturated fatty acids (long PUFAs). The blood-brain barrier (BBB) is formed by the brain endothelial cells under the inductive influence of brain cells, especially perivascular glia, and coculture of endothelial cells and glial cells has been used to examine this induction. The objective of this study was to investigate whether C6 glioma cells are able to influence the lipid composition and shift the fatty acid (FA) patterns of the BBB model cell lines RBE4 and ECV304 toward the in vivo situation. Lipid classes of the three cell lines were analyzed by thin-layer chromatography and lipid FA patterns by high-performance liquid chromatography. Only ECV304 cells showed altered lipid composition in coculture with C6 cells. The fractions of triglycerides and cholesteryl esters (depending on the support filter) were about twice as high in coculture as when the cells were grown alone. Triglyceride fractions reached 13 to 15% of total lipids in coculture. The three cell lines showed an increase in the percentage of long PUFAs with respect to unsaturated FAs, mainly because of an increase in the percentages of arachidonic acid, all cis-7,10,13,16-docosatetraenoic acid, and all cis-7,10,13,16,19-docosapentaenoic acid. It is concluded that glioma C6 cells are able to induce a more in vivo-like FA pattern in BBB cell culture models. However, changes were not significant for the individual PUFAs, and their levels did not reach in vivo values. 相似文献