La paléoflore autuno-stéphanienne de la région de Demnate(Est de Marrakech-Maroc)

The paleoflora described and pictured in this paper has been collected in two deposits of the area of Demnate: the OT6 bis drilling in the oriental Haouz and an exposure in the Rhojdamas (High-Atlas). Two completely different associations have been characterized: one hygrophilous and autochtonous of the «Stephanian type and the other mesoxerophilous and allochtonous (vegetation of the upland?) of the «Autunian type. In the OT6 bis drilling, the two floras alternate so that it is impossible to draw a precise boundary between the Stephanian and Autunian. In the Rhojdamas, only the Autunian flora has been identified. Such a combination of the two floras has similarly been recorded in several stephanian basins of the French Massif Central.     

Discovery of an Autunian macroflora and lithostratigraphic re-investigation on the western border of the Lodève Permian basin (Mont Sénégra,Hérault,France). Paleoenvironmental implications

Re-investigation of the western edge of the Lodève basin allows reassignment of one sandstone-conglomerate formation previously identified as “terminal Stephanian” to Early Autunian. The existence of two unconformable (Stephanian and Autunian) megasequences, separated by a sedimentary gap, which had been rejected, is thus re-affirmed. The authors also found, less than 20 m above the basal Autunian conglomerate, a macroflora with taxa characteristic of the famous Tuilières flora from a site, located in the eastern part of the basin near Lodève, in the Grey Autunian group. This confirms that the new Mont Sénégra fossiliferous beds belong to the Lower Autunian. Moreover, the taxonomic differences between these plants and those from the underlying coal-bearing Stephanian beds indicate an important change in the vegetation between the Stephanian and the Autunian. The first Autunian sequences were initially deposited within a distal alluvial fan environment, which developed vertically into a floodplain, within an active volcanic context.     

Pennsylvanian palynofloras from the Itu rhythmites (Itararé Subgroup, Paraná Basin) in São Paulo State, Brazil

The rhythmites of Itu present the best exposures of glacial varvites known in the Paraná Basin, and constitute a classic geological monument related to the Late Palaeozoic Gondwanan glaciation. Palynological results in this paper are based on rhythmites from two quarries in Itu area, central-eastern State of São Paulo, Brazil, including correlate levels of the “Itu Varvite Park”, as well as based on samples from the borehole IT-IG-85 (at 171 and 228 m) drilled also in the Itu City. Well-preserved indigenous miospores and microphytoplankton elements have been recorded. The former comprise 15 spore species and 19 pollen species, of which three are recorded for the first time in the Brazilian part of the Paraná Basin (Verrucosisporites cf. V. andersonii, Convolutispora archangelskyi and Caheniasaccites verrucosus). Microalgae include prasinophyceans (Leiosphaeridia sp., Tasmanites sp., Deusilites tenuistriatus), chlorophyceans (Botryococcus braunii) and zignemataceans (Tetraporina). The palynological content confirms a late Pennsylvanian (Kasimovian/Gzhelian) age for these rhythmites, which are assigned to the Crucisaccites monoletus interval Zone, based on the record of the eponymous pollen species and Scheuringipollenites maximus. Although very scarce, prasinophycean algae suggest low salinity marine conditions.     

Mutual affinities of Santalalean families

The paper represents a reassessment of familial relationships within the Order Santalales. Rafflesiaceae, Hydnoraceae, and Balanophoraceae are excluded from the order, and their reproductive similarities with some Santalales regarded as evolutionary convergence. The exclusion of Grubbiaceae from Santalales sponsored by other workers is accepted, as is the reassignment to other families of the two only genera of Octoknemaceae. The remaining members of the order are placed into six families, viz. Olacaceae (possibly representing several smaller families); Santalaceae; Loranthaceae (sensu stricto); Viscaceae (sensu stricto); Myzodendraceae ; and Eremolepidaceae (containingEremolepis, Eubrachion, andAntidaphne). It is suggested thatLepidoceras, formerly placed near the above three genera, is more properly placed in Loranthaceae (sensu stricto) nearTupeia. The proposed scheme of affinities of the families of Santalales, as reconstituted, is based to a large extent on inflorescence structure, but is supported by floral morphology and other features. The thesis is defended that Loranthaceae (sensu stricto), Santalaceae, Myzodendraceae, and Eremolepidaceae represent four inde pendent lines of development from portions of the Olacacean complex. Viscaceae (sensu stricto) may be derived directly from Santalaceae.     

The fossil turtles of Greece: An overview of taxonomy and distribution

Turtle remains are common in the Miocene-Holocene deposits of Greece, and are a key focus of the growing research interest in Neogene herpetofaunas from the Aegean region. Some of the most important finds include one of Europe's stratigraphically youngest pleurodiran taxa, Nostimochelone lampra, from the Early Miocene of Macedonia, together with arguably the richest record of fossil tortoises from the Eastern Mediterranean. This incorporates the presently oldest definitive representatives of the quintessential genus Testudo sensu stricto from the Late Miocene of Attica and Macedonia, and numerous specimens of the colossal (carapace ∼2 m-length) testudinid Cheirogaster from Late Miocene-Late Pliocene sediments in southern and northern Greece, as well as on the eastern Aegean islands of Samos and Lesvos. Tantalising, but as yet unconfirmed Miocene accounts of the geoemydid Mauremys in Macedonia, and indeterminate emydid-like remains from Euboea, also provide potentially significant range extensions. Although hampered by a historically sparse documentation, the fossil turtles of Greece are a significant resource that record both assemblage changes and the origin of modern lineages.     

Circumscription of the order Ectocarpales (Phaeophyceae): bibliographical synthesis and molecular evidence

Phylogenetic analyses of 27 brown algae including the type genera of the orders Chordariales, Dictyosiphonales, Ectocarpales sensu stricto, and Scytosiphonales, using partial SSU + LSU combined rDNA sequence data, supports a broadly circumscribed order Ectocarpales. This order is redefined to include taxa possessing an exserted, pedunculated pyrenoid. Previous then taxa were placed in the Ectocarpales sensu stricto, the Chordariales, the Dictyosiphonales, or the Scytosiphonales. Algae either lacking a pyrenoid, and sometimes included in the Ectocarpales (Tilopteridales, Ralfsiales sensu Nakamura), or which possess a non-pedunculated pyrenoid (such as those placed in the recently proposed order Scytothamnales, as well as Asteronema, Asterocladon and Bachelotia), are excluded from the Ectocarpales.     

Evolutionary Aspects of Emerging Lyme Disease in Canada

In North America, Lyme disease (LD) is a tick-borne zoonosis caused by the spirochete bacterium Borrelia burgdorferi sensu stricto, which is maintained by wildlife. Tick vectors and bacteria are currently spreading into Canada and causing increasing numbers of cases of LD in humans and raising a pressing need for public health responses. There is no vaccine, and LD prevention depends on knowing who is at risk and informing them how to protect themselves from infection. Recently, it was found in the United States that some strains of B. burgdorferi sensu stricto cause severe disease, whereas others cause mild, self-limiting disease. While many strains occurring in the United States also occur in Canada, strains in some parts of Canada are different from those in the United States. We therefore recognize a need to identify which strains specific to Canada can cause severe disease and to characterize their geographic distribution to determine which Canadians are particularly at risk. In this review, we summarize the history of emergence of LD in North America, our current knowledge of B. burgdorferi sensu stricto diversity, its intriguing origins in the ecology and evolution of the bacterium, and its importance for the epidemiology and clinical and laboratory diagnosis of LD. We propose methods for investigating associations between B. burgdorferi sensu stricto diversity, ecology, and pathogenicity and for developing predictive tools to guide public health interventions. We also highlight the emergence of B. burgdorferi sensu stricto in Canada as a unique opportunity for exploring the evolutionary aspects of tick-borne pathogen emergence.     

Paranthropus boisei: An example of evolutionary stasis?

Of the presently recognised early hominid species, Paranthropus boisei is one of the better known from the fossil record and arguably the most distinctive; the latter interpretation rests on the numbers of apparently derived characters it incorporates. The species as traditionally diagnosed is distributed across approximately one million years and is presently confined to samples from East African sites. The hypodigm has been examined for evidence of intraspecific phyletic evolution, with particular emphasis on the areas best represented in the fossil record, namely the teeth and mandible. The results of this examination of 55 mandibular and dental variables, which uses the Γ test statistic for the detection of trend, and nonparametric spline regression (Loess regression) for investigating pattern and rate of temporal change, show that within Paranthropus boisei sensu stricto most evidence of temporally related morphological trends relates to the morphology of the P₄ crown. There is little or no evidence of any tendency to increase in overall size through time. Fossils from the Omo Shungura Formation and West Turkana which have been recovered from a time period earlier than the P. boisei sensu stricto hypodigm resemble the latter taxon in some features, but differ from it in aspects of cranial morphology. There is insufficient fossil evidence of the earlier taxon to tell whether it changes through time, but when trends of 47 mandibular and dental variables are assessed across the combined East African “robust” australopithecine sample, there is evidence for a relatively abrupt change around 2.2–2.3 Myr in approximately 25% of the dental and mandibular remains. Some of these changes correspond with the temporal trends within P. boisei sensu stricto, but others, such as mandible height, do not. If the earlier material is ancestral to P. boisei sensu stricto, evidence from the teeth and jaws is consistent with a punctuated origin for the latter taxon. © 1994 Wiley-Liss, Inc.     

The Carboniferous floras of the Iberian Peninsula: A synthesis with geological connotations

A general review is presented of the Carboniferous floral records in the Iberian Peninsula in the context of the geological history and distribution of the different basins. Mississippian floras are found in Sierra Morena, where major strike-slip faults brought in terranes of diverse provenance. Lower Pennsylvanian floras are represented in the Peñarroya-Belmez-Espiel (Córdoba) and Villanueva del Río y Minas (Sevilla) coalfields of SW Spain (also strike-slip controlled), at La Camocha, near Gijón (Asturias), and in other parts of northern Spain. Middle Pennsylvanian is represented near Oporto, but more completely in the Central Asturian Coalfield, as well as other, more limited localities in NW Spain and the Pyrenees. Upper Pennsylvanian (Stephanian Stage-Cantabrian to Stephanian B substages) floras are splendidly represented in NW Spain. Uppermost Pennsylvanian (Stephanian C-Autunian) floras are present in the strike-slip controlled Douro and Buçaco basins of North Portugal, the Pyrenees, Central Spain (Ciudad Real, Guadalajara, Zaragoza), and SW Spain (Guadalcanal and Valdeviar in Sevilla province).A complete succession of megafloral zones is presented. This includes a new Annularia spicata Zone at the top of the Pennsylvanian (equivalent to middle to upper Autunian which has been often attributed to the Lower Permian). The information is summarised in charts compiled from a selection of the most significant species. A number of floral elements are illustrated including the zonal indices. Brief taxonomic comments are provided in the Appendix.     

Quantitative real time PCR assays for the enumeration of Saccharomyces cerevisiae and the Saccharomyces sensu stricto complex in human feces

There have been an increasing number of reports of yeast systemic infection involving Saccharomyces cerevisiae strains. The development of a rapid and reliable diagnostic tool is therefore warranted in order to explore the distribution of S. cerevisiae as an opportunistic pathogen in humans. In this study, we designed and validated five primer sets targeting the 26S rRNA gene of S. cerevisiae and the S. sensu stricto complex using 26 yeast strains. Among them, two sets of primers specifically amplified the 26S rRNA gene and the ITS region of S. cerevisiae strains, and three sets were specific for amplifying the same genes in the S. sensu stricto complex. After determining the optimal conditions of two primer pairs for quantitative real time PCR, human fecal samples were analyzed to examine the distribution of S. cerevisiae and the S. sensu stricto complex. It was possible to detect a single cell of S. cerevisiae in environmental sample. Qualitative PCR revealed that out of eleven fecal samples tested, one sample contained S. cerevisiae and four samples contained the S. sensu stricto complex. Quantitative real time PCR revealed that the target gene copy numbers of S. cerevisiae and the S. sensu stricto complex were 0.84 and 2.44 respectively, in 1 ng of DNA from the bulk fecal community.     

Species distribution and antifungal susceptibility among clinical isolates of Candida parapsilosis complex from India

Background

Candida parapsilosis is recognized as a species complex: Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis are three distinct but closely related species.

Lipid diversity in clostridia

Studies of the lipidomes of twenty-one species of clostridia have revealed considerable diversity. Even among those species now defined as Clostridium sensu stricto, which are related to Clostridium butyricum, the type species, lipid analysis has shown that a number of distinct clades have characteristic polar lipids. All species of Clostridium sensu stricto have phosphatidylethanolamine, phosphatidylglycerol and cardiolipin which are present as all acyl or alk-1′-enyl acyl (plasmalogen) species. In addition, almost every clade has specialized polar lipids. For example, the group closely related to Clostridium beijerinckii and several other solventogenic species has glycerol acetals of plasmenylethanolamine, which protects the membrane bilayer arrangement when the lipids are highly unsaturated or in the presence of solvents. The group related to Clostridium novyi has aminoacyl-phosphatidylglycerol, which protects these pathogens from cationic antimicrobial peptides (CAMPs) of innate immunity. Clostridium botulinum species, which fall into several groups, align with these clades, and have the same specific lipids. This review will present the current state of knowledge on clostridial lipids.     

Sporothrix globosa,a pathogenic fungus with widespread geographical distribution

Sporothrix globosa, reported from the USA, Europe, and Asia, is a recently described pathogenic species morphologically similar to Sporothrix schenckii. In this study, the phylogenetic affinities of 32 clinical and environmental isolates morphologically identified as S. schenckii, from Mexico, Guatemala, and Colombia, were assessed by cladistic analysis of partial sequences of the calmodulin gene using the maximum parsimony and neighbor-joining methods. The study revealed that one out of 25 isolates from Mexico (4%), one out of three isolates from Guatemala (33.3%), and two out of four isolates from Colombia (50%) belonged to S. globosa, while the other isolates belonged to S. schenckii sensu stricto. This is the first record of S. globosa from Mexico, and Central and South America.     

The earliest record of the genus Cola (Malvaceae sensu lato: Sterculioideae) from the Late Oligocene (28–27 Ma) of Ethiopia and leaf characteristics within the genus

A fossil leaf compression from the Late Oligocene (28–27 Ma) of northwestern Ethiopia is the earliest record of the African endemic moist tropical forest genus Cola (Malvaceae sensu lato: Sterculioideae). Based on leaf and epidermal morphology, the fossil is considered to be very similar to two extant Guineo-Congolian species but differences warrant designation of a new species. This study also includes a review of the fossil record of Cola, a comprehensive summary of leaf characteristics within several extant species of Cola, Octolobus, and Pterygota, and a brief discussion of the paleogeographic implications of the fossil species affinity and occurrence in Ethiopia.     

Experimental Infection of Ornithodoros erraticus sensu stricto with Two Portuguese African Swine Fever Virus Strains. Study of Factors Involved in the Dynamics of Infection in Ticks

African swine fever (ASF) is a frequently devastating hemorrhagic disease of domestic pigs and wild boar and Ornithodoros erraticus sensu stricto argasid ticks are the only biological vectors of African swine fever virus (ASFV) known to occur in Europe. Recently this disease emerged in Eastern Europe and Russian Federation, showing a huge potential for a rapid spread between countries. There is some risk of re-emergence of ASF in the countries where these ticks exist, that can contribute for the persistence of infection and compromise control measures. In this study we aimed to identify factors that determine the probability of infection and its dynamics in the tick vector Ornithodoros erraticus sensu stricto, with two Portuguese strains of ASFV. Our results suggest that these ticks have a high likelihood of excreting the two haemadsorbing ASF viruses of different host origins and that, in field surveys, the analysis of adults and 5^th nymphal stage can provide the best chance of detecting virus infection. The results also indicate that infection of pigs with highly virulent ASF viruses will promote higher rates of infection and a higher likelihood for virus excretion by ticks. Nevertheless, there is also a risk, although lower, that ticks can become infected on pigs that have overcome the acute phase of infection, which was simulated in our study by membrane feeding ticks with low titres of virus. We believe these results can be valuable in designing and interpreting the results of ASF control programmes, and future work can also be undertaken as our dataset is released under open access, to perform studies in risk assessment for ASFV persistence in a region where O. erraticus sensu stricto ticks are present.     

Germination performance of congeneric Styrax species from the Cerrado sensu lato areas and their distribution pattern in different physiognomies

When studying congeneric species, it is of reasonable importance to understand different ecophysiological performances which might determine the distribution of species in habitats with different natural resources. Styrax ferrugineus is exclusive and well adapted to the Brazilian Cerrado sensu stricto (s. str.); S. camporum is widely distributed in the Cerrado sensu lato (s. l.) areas, with young trees being observed at the edge of cerradão and other vegetation fragments; and S. pohlii occurs in permanently waterlogged soils of the Cerrado region, such as those of riparian forests. We tested the hypothesis that the higher the soil water content in the physiognomic gradient of the vegetation, the higher is the germination success of S. pohlii, but the lower is the germination success of S. ferrugineus. We also discuss whether gap conditions inside a cerradão fragment imply a high germination rates of seeds of S. camporum. Seeds from each of the three species were buried within nylon bags containing soil from the respective sites. Burial occurred in a Cerrado s. str., in understory and gap conditions of a cerradão, and in the understory of a riparian forest fragment, and lasted for 60, 120, 180 and 240 days, respectively, after the fruit dispersal time of each of the three species. After 60 days, a relationship was found showing that the percentage of germinated seeds diminished, and the percentage of damaged seeds increased as soil water content increased (Cerrado s. str. < cerradão gap < cerradão understory ? riparian forest). S. camporum still showed viable seeds 60 days after burial (DAB), and germinated seeds 120 DAB, indicating that it needed a longer time to germinate, which might be associated to its thicker seed coat, in relation to the other two species. The germination performance of each of the three species was the same in the gap and understory conditions of the cerradão. The higher concentration of adult S. camporum plants at the edge of vegetation fragments is not related to a particular high germination performance and seedling establishment.     

Diversity dynamics of Zosterophyllopsida

The Zosterophyllopsida were major contributors to the diversification of early land plants. We present the first detailed analysis of the diversity dynamics of these plants from an updated database of all currently recognized zosterophyllopsid species. A set of quantitative methods classically used in palaeodiversity studies was applied to two data sets. The first one, ‘Zosterophyllopsida sensu stricto’, corresponds to the clade identified by Hao & Xue (The Early Devonian Posongchong Flora of Yunnan. (2013), Science Press). In the second, called ‘Zosterophyllopsida sensu lato’, barinophytalean‐type plants and taxa for which zosterophyllopsid affinities are suspected are added. The number of localities is used to explore sampling bias. Results show that sampling effect is minimal for the Early Devonian. For this time interval, both data sets record consistent patterns of changes suggesting that, whatever their affinities, all taxa included in the Zosterophyllopsida sensu lato show similar evolutionary trends. The diversity dynamics of zosterophyllopsids are characterized by a radiation during the Lochkovian, maximal values in the Pragian and a decline starting in the Emsian. The proportion of zosterophyllalean taxa with terminal sporangia is high until the Late Lochkovian when gosslingialean taxa without terminal sporangia evolved. During the Middle and Late Devonian, when diversity patterns are strongly affected by sampling, zosterophyllopsid diversity is low and characterized by a high proportion of barinophytacean and gosslingialean taxa, the latter becoming extinct in the Early Frasnian.     

Delineation of Paraburkholderia tuberum sensu stricto and description of Paraburkholderia podalyriae sp. nov. nodulating the South African legume Podalyria calyptrata

Since the discovery of Paraburkholderia tuberum, an indigenous South African species and one of the first beta-rhizobia described, several other South African rhizobial Paraburkholderia species have been recognized. Here, we investigate the taxonomic status of 31 rhizobial isolates from the root nodules of diverse South African legume hosts in the Core Cape Subregion, which were initially identified as P. tuberum. These isolates originate from the root nodules of genera in the Papilionoideae as well as Vachellia karroo, from the subfamily Caesalpinioideae. Genealogical concordance analysis of five loci allowed delineation of the isolates into two putative species clusters (A and B). Cluster A included P. tuberum STM678^T, suggesting that this monophyletic group represents P. tuberum sensu stricto. Cluster B grouped sister to P. tuberum and included isolates from the Paarl Rock Nature Reserve in the Western Cape Province. Average Nucleotide Identity (ANI) analysis further confirmed that isolates of Cluster A shared high genome similarity with P. tuberum STM678^T compared to Cluster B and other Paraburkholderia species. The members of Cluster B associated with a single species of Podalyria, P. calyptrata. For this new taxon we accordingly propose the name Paraburkholderia podalyriae sp. nov., with the type strain WC7.3b^T (= LMG 31413^T; SARCC 750^T). Based on our nodA and nifH phylogenies, P. podalyriae sp. nov. and strains of P. tuberum sensu stricto (including one from V. karroo) belong to symbiovar africana, the symbiotic loci of which have a separate evolutionary origin to those of Central and South American Paraburkholderia strains.