共查询到20条相似文献,搜索用时 27 毫秒
1.
Ana Jiménez 《植物学报(英文版)》2007,49(7):982-992
The effects of NaCl stress on the activity of anti-oxidant enzymes (superoxide dismutase, catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), monodehydroascorbate reductase, dehydroascorbate reductase (DHAR), and glutathione reductase (GR)), anti-oxidant molecules (ascorbate and glutathione), and parameters of oxidative stress (malondialdehyde (MDA), electrolyte leakage, and H2O2 concentrations) were investigated in Cakile maritima, a halophyte frequent along the Tunisian seashore. Seedlings were grown in the presence of salt (100, 200, and 400 mmol/L NaCl). Plants were harvested periodically over 20 days. Growth was maximal in the presence of 0-100 mmol/L NaCl. At 400 mmol/L NaCl, growth decreased significantly. The salt tolerance of C. maritima, at moderate salinities, was associated with the lowest values of the parameters indicative of oxidative stress, namely the highest activities of POD, CAT, APX, DHAR, and GR and high tissue content of ascorbate and glutathione. However, prolonged exposure to high salinity resulted in a decrease in anti-oxidant activities and high MDA content, electrolyte leakage, and H2O2 concentrations. These results suggest that anti-oxidant systems participate in the tolerance of C. maritima to moderate salinities. 相似文献
2.
Nader Ben Amor Ana Jimenez Wided Megdiche MarianneLundqvist FranciscaSevilla Chedly Abdelly 《Acta Botanica Sinica》2007,(7)
The effects of NaCl stress on the activity of anti-oxidant enzymes (superoxide dismutase, catalase (CAT),peroxidase (POD),ascorbate peroxidase (APX), monodehydroascorbate reductase, dehydroascorbate reductase (DHAR), and glutathionereductase (GR)), anti-oxidant molecules (ascorbate and glutathione), and parameters of oxidative stress (malondialdehyde(MDA), electrolyte leakage, and H_2O_2 concentrations) were investigated in Cakile maritima, a halophyte frequent along theTunisian seashore. Seedlings were grown in the presence of salt (100, 200, and 400 mmol/L NaCI). Plants were harvestedperiodically over 20 days. Growth was maximal in the presence of 0-100 mmol/L NaCl. At 400 mmol/L NaCl, growthdecreased significantly. The salt tolerance of C. maritima, at moderate salinities, was associated with the lowest values ofthe parameters indicative of oxidative stress, namely the highest activities of POD, CAT, APX, DHAR, and GR and high tissuecontent of ascorbate and glutathione. However, prolonged exposure to high salinity resulted in a decrease in anti-oxidantactivities and high MDA content, electrolyte leakage, and H_2O_2 concentrations. These results suggest that anti-oxidantsystems participate in the tolerance of C. maritima to moderate salinities. 相似文献
3.
Coronatine alleviates salinity stress in cotton by improving the antioxidative defense system and radical-scavenging activity 总被引:5,自引:0,他引:5
Coronatine (COR) is a chlorosis-inducing phytotoxin that mimics some biological activities of methyl jasmonate. This study investigated whether COR confers salinity tolerance to cotton and whether such tolerance is correlated with changes in the activity of antioxidant enzymes. COR at 0.01muM was applied hydroponically to cotton seedlings at the two-leaf stage for 24h. A salinity stress of 150mM NaCl was imposed after completion of COR treatment for 15d. Salinity stress reduced biomass of seedlings and increased leaf superoxide radicals, hydrogen peroxide, lipid peroxidation, and electrolyte leakage. Activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and glutathione reductase (GR), and of the stable free radical, 1,1-diphenyl-2-picrylhydrazyl (DPPH), scavenging activity were altered by salinity to varying degrees. Pretreatment with COR increased the activities of CAT, POD, GR, and DPPH scavenging activity in leaf tissues of salinity-stressed seedlings. Thus, COR might reduce the production of reactive oxygen species by activating antioxidant enzymes and DPPH-radical scavenging, thereby preventing membrane peroxidation and denaturation of bio-molecules. 相似文献
4.
Nitika Arora Renu Bhardwaj Priyanka Sharma Hardesh Kumar Arora 《Acta Physiologiae Plantarum》2008,30(6):833-839
The effects of 28-homobrassinolide (28-homoBL) on seedling growth, lipid peroxidation and antioxidative enzyme activities
in the seedlings of Zea mays L. (var. Partap-1) under salt (NaCl) stress were studied. The surface-sterilized seeds were germinated in petriplates containing
different concentrations of NaCl (25, 50, 75 and 100 mM) only, 28-homoBL (10−7, 10−9 and 10−11 M) only and NaCl supplemented with 28-homoBL for 7 days. The activities of superoxide dismutase (SOD, EC 1.15.1.1), guaiacol
peroxidase (POD, EC 1.11.1.7), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APOX, EC 1.11.1.11) and glutathione reductase
(EC 1.6.4.2) were analysed in 7 day-old seedlings. It was observed that 28-homoBL treatments reduced the toxicity of salt
on seedling growth considerably. Lipid peroxidation level was significantly increased under saline stress, but lowered with
28-homoBL applications revealing less oxidative damage. Further 28-homoBL treatments to the seedlings showed an enhancement
in activities of SOD, POD, CAT and APOX. The activities of all antioxidative enzymes were further increased in seedlings treated
with solution containing 28-homoBL and salt together as compared to seedlings treated with different concentration of salt
solution only. 相似文献
5.
NaCl胁迫下高纬度移植桐花树幼苗的生理生态效应 总被引:1,自引:0,他引:1
通过砂培试验,研究了不同浓度NaCl(0、100、200、300和400 mmol·L-1)处理对高纬度移植桐花树幼苗生物量、离子吸收、碳氮代谢、叶片光合色素和叶片抗氧化系统的影响.结果表明:100 mmol·L-1NaCl处理对桐花树生长有轻微的促进作用,当NaCl浓度达到300mmol·L-1时,桐花树根、茎、叶器官的干鲜质量、根冠比、株高和基径均显著下降.高盐胁迫抑制了叶片抗氧化系统中超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性,提高了丙二醛含量,降低了叶片中叶绿素、类胡萝卜素总量以及根茎叶的可溶性总糖和游离氨基酸总量.不同浓度NaCl胁迫下,桐花树根茎叶中Na+含量快速上升,K+含量相对下降,K+/Na+快速下降,导致各器官中离子平衡失调.当NaCl浓度高于300 mmol·L-1时,桐花树根茎叶的碳氮代谢运转失调,抑制了植株的正常生长,导致各器官的生物量显著下降. 相似文献
6.
外源一氧化氮对镉胁迫下绿豆幼苗根尖抗氧化酶的影响 总被引:3,自引:0,他引:3
采用水培法研究外源一氧化氮对镉(Cd)胁迫下绿豆幼苗根尖抗氧化酶活性的影响。结果表明:0.01mmol/L和0.1mmol/L一氧化氮供体硝普钠(sodium nitroprusside,SNP)显著促进上胚轴生长,1mmol/LSNP则抑制绿豆幼苗生长。Cd单独处理抑制根尖抗坏血酸过氧化物酶(ascorbate peroxidase,APX)和超氧化物歧化酶(superoxide dismutase,SOD)活性而刺激脂氧合酶(lipoxygenase,LOX)、谷胱甘肽转硫酶(glutathione S-transferase,GST)、谷胱甘肽还原酶(glutathione reductase,GR)和过氧化物酶(guaiacol peroxidase,POD)活性上升。0.1mmol/LSNP预处理能够明显缓解Cd对根生长的抑制,降低根尖中MDA含量,提高根尖APX和SOD活性,降低LOX和POD活性,但不影响GST和GR活性。 相似文献
7.
Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.) 总被引:26,自引:0,他引:26
Two contrasting barley (Hordeum vulgare L.) cultivars, i.e. Kepin No.7 (salt sensitive) and Jian 4 (salt tolerant), were grown hydroponically to study the effect of exogenous silicon (Si) on time dependent changes of the activities of major antioxidant enzymes and of lipid peroxidation in roots under salt stress. Enzymes included: superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and glutathione reductase (GR). Three treatments with three replicates were investigated consisting of a control (basal nutrients with neither NaCl nor Si added), 120 mmol/L-1 NaCl, and 120 mmol/L-1 NaCl +1.0 mmol/L-1 Si. Plant roots were harvested 2, 4 and 6 days after treatment and assayed for activities of the antioxidant enzymes and the concentrations of reduced glutathione (GSH) and malondialdehyde (MDA), and electrolytic leakage percentage (ELP). The activities of SOD, POD and CAT in roots of salt-stressed plants were significantly stimulated at Day 2 compared to control plants, but considerably decreased at Day 4 and onward. GR activity in roots of salt-stressed plants remained unchanged at Day 2, but significantly decreased at Day 4 and onward. However, exogenous Si significantly enhanced these enzyme activities in roots of salt-stressed plants compared to Si-deprived salt treatments. This Si effect was time-dependent and became stronger as the experiments continued. The tendency of change in the activities of antioxidant enzymes and the concentration of GSH coincided with the concentration of MDA, the end product of lipid peroxidation, and the ELP. Higher activities of antioxidant enzymes, and higher concentration of GSH, but lower concentration of MDA and lower ELP were noted in cultivar Jian 4 compared to Kepin No. 7, implying genotypic differences with Jian 4 being less susceptible to stress-dependent membrane lipid peroxidation. The effects of Si-enhanced salt tolerance are discussed with respect to cell membrane integrity, stability and function in barley. 相似文献
8.
外源一氧化氮对氯化钠处理下秋茄幼苗抗氧化系统的调节效应 总被引:4,自引:0,他引:4
研究了外源一氧化氮(NO)供体硝普钠(SNP)对NaCl处理下红树植物秋茄(Kan-deliacandel)幼苗叶片中抗氧化酶活性、抗氧化物质及脯氨酸含量的影响。结果表明:NaCl处理下,秋茄幼苗叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)等4种活性氧清除酶的活性均受到明显抑制(P<0.05),SNP可以不同程度地恢复SOD、POD、CAT的活性,但对APX活性影响不大;SNP提高谷胱甘肽(GSH)及类胡萝卜素(Car)的含量,促进脯氨酸含量的上升,显著降低叶片中过氧化氢(H2O2)和丙二醛(MDA)的累积。表明外源NO可以缓解NaCl处理诱导的秋茄幼苗叶片氧化损伤,降低膜脂过氧化水平,有利于秋茄适应盐生环境。 相似文献
9.
采用蛭石栽培试验,在100mmol.L-1 NaCl胁迫下,对耐盐性不同的两个菜用大豆品种种子膨大、抗氧化系统及渗透调节物质进行了研究。结果显示:(1)耐盐品种‘绿领特早’种子干质量及大小的增加在NaCl胁迫15d时未受显著影响,而盐敏感品种‘理想高产95-1’种子的膨大受到了显著抑制。(2)NaCl胁迫导致菜用大豆种子超氧阴离子(O2.-)、过氧化氢(H2O2)及丙二醛(MDA)的过量积累,但‘绿领特早’种子的相对积累量均低于‘理想高产95-1’。(3)与‘理想高产95-1’相比,‘绿领特早’在NaCl胁迫期间维持了相对较高的超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)及谷胱甘肽还原酶(GR)活性,以及较高的抗坏血酸(AsA)、可溶性蛋白和可溶性糖含量;胁迫中期维持了相对较高的过氧化物酶(POD)活性,胁迫后期维持了相对较高的过氧化氢酶(CAT)活性;胁迫前中期维持了相对较高的谷胱甘肽(GSH)含量。研究发现,‘绿领特早’种子在NaCl胁迫期间能够保持较高且协调平衡的保护酶SOD、POD、CAT活性和较高的AsA-GSH循环效率,同时维持了较强的渗透调节能力,有效抑制了O2.-和H2O2的过量积累,进而有效降低了细胞膜质过氧化程度,这可能是其耐盐性较强的重要原因之一。 相似文献
10.
一氧化氮对盐胁迫下小麦幼苗根生长和氧化损伤的影响 总被引:45,自引:2,他引:45
0.05和0.10 mmol/L一氧化氮(NO)供体硝普钠(sodium mtropmsside,SNP)处理明显减轻NaCl浓度为150 mmo1/L左右的盐胁迫对小麦幼苗根生长的抑制效应,其中0.05mmol/L的SNP效果最明显;0.30mmol/L以上的SNP处理对根抑制无明显缓解作用;当NaCl浓度大于300 mmol/L时,各种浓度的SNP均不能减轻盐胁迫对根生长的抑制.以N O清除剂血红蛋白(hemoglobin,Hb)以及NOx-,K3Fe(CN)6等为对照,观察到0.05 mmol/L的SNP能不同程度地提高150mmo/L盐胁迫下小麦幼苗根尖细胞中超氧化物歧化酶(SOD)、过氧化物酶(POD)和抗坏血酸过氧化物酶(ascorbateperoxidase,APX)活性,明显降低MDA、H2O2和O2-.的积累,阻断盐胁迫诱导的根尖细胞DNA片段化,表明NO能有效缓解盐胁迫引起的小麦幼苗根尖细胞的氧化损伤. 相似文献
11.
外源水杨酸对NaCl胁迫下大豆种子萌发和幼苗生长生理的影响 总被引:2,自引:0,他引:2
以大豆种子、幼苗为试验材料,采用砂培的方法,研究了0.2mmol·L-1外源水杨酸(SA)对100mmol·L-1 NaCl胁迫下大豆种子萌发、幼苗形态及生物量、膜脂过氧化和抗氧化酶活性的影响。结果显示:NaCl胁迫下,大豆种子萌发和幼苗生长受到显著抑制,且随着胁迫时间的延长(0~3d),大豆幼苗相对电解质渗漏率、硫代巴比妥酸活性产物(TBARS)含量显著升高,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性均明显降低。外源SA促进NaCl胁迫下大豆种子萌发和根茎生长,增加幼苗生物量积累,降低幼苗叶片相对电解质渗漏率和TBARS含量,增强其叶片SOD、CAT、APX活性。研究表明,NaCl胁迫能显著抑制大豆种子萌发和幼苗生长,而一定浓度的外源SA能有效提高NaCl胁迫下大豆种子活力及幼苗抗氧化酶活性,减轻膜脂过氧化程度,缓解NaCl胁迫所造成的伤害,提高大豆幼苗抗盐胁迫的能力。 相似文献
12.
13.
甜菜碱对NaCl胁迫下小麦细胞保护酶活性的影响 总被引:1,自引:0,他引:1
本文以小麦为材料,研究甜菜碱对NaCl胁迫下小麦幼苗细胞保护酶系统的影响。结果表明,外源甜菜碱能够提高NaCl胁迫下小麦幼苗超氧化物歧化酶、过氧化物酶等细胞保护酶的活性,抑制过氧化作用产物丙二醛的积累,降低叶片质膜透性和盐害对细胞膜的伤害。所有这些变化都有利于提高NaCl胁迫下小麦幼苗细胞膜的稳定性、完整性和对NaCl胁迫的适应性。 相似文献
14.
Jing Zhang Xiaohui Duan Fan Ding HaiZhen Ma Tengguo Zhang Yingli Yang 《Protoplasma》2014,251(4):771-780
This study aimed to investigate the inhibitory mechanism of root growth and to compare antioxidative responses in two wheat cultivars, drought-tolerant Ningchun and drought-sensitive Xihan, exposed to different NaCl concentrations. Ningchun exhibited lower germination rate, seedling growth, and lipid peroxidation than Xihan when exposed to salinity. The loss of cell viability was correlated with the inhibition of root growth induced by NaCl stress. Moreover, treatments with H2O2 scavenger dimethylthiourea and catalase (CAT) partly blocked salinity-induced negative effects on root growth and cell viability. Besides, the enhancement of superoxide radical and H2O2 levels, and the stimulation of CAT and diamine oxidase (DAO) as well as the inhibition of glutathione reductase (GR) were observed in two wheat roots treated with salinity. However, hydroxyl radical content increased only in Xihan roots under NaCl treatment, and the changes of soluble peroxidase (POD), ascorbate peroxidase (APX), superoxide dismutase (SOD), and cell-wall-bound POD activities were different in drought-tolerant Ningchun and drought-sensitive Xihan exposed to different NaCl concentrations. In conclusion, salinity might induce the loss of cell viability via a pathway associated with extracellular H2O2 generation, which was the primary reason leading to the inhibition of root growth in two wheat cultivars. Here, it was also suggested that increased H2O2 accumulation in the roots of drought-tolerant Ningchun might be due to decreased POD and GR activities as well as enhanced cell-wall-bound POD and DAO ones, while the inhibition of APX and GR as well as the stimulation of SOD and DAO was responsible for the elevation of H2O2 level in drought-sensitive Xihan roots. 相似文献
15.
Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses 总被引:2,自引:0,他引:2
Wen-Bin Wang Yun-Hee Kim Haeng-Soon Lee Ki-Yong Kim Xi-Ping Deng Sang-Soo Kwak 《Plant Physiology and Biochemistry》2009,47(7):570-577
To understand the adaptability of alfalfa (Medicago sativa L.) to environmental stresses, we analyzed the activity of several antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT), in alfalfa shoots and roots subjected to salt and drought stresses during germination. The germination rate of six alfalfa cultivars was comparatively studied under 200 mM NaCl or 35% PEG treatment. Alfalfa Xinmu No. 1 and Northstar varieties were selected as stress-tolerant and -sensitive cultivars, respectively, and were used for further characterization. After NaCl or PEG treatment, Xinmu No. 1 showed enhanced seedling growth, compared with Northstar. Xinmu No. 1 also exhibited low levels of hydrogen peroxide (H2O2) production and lipid peroxidation, compared with Northstar. In addition, Xinmu No. 1 showed higher enzymatic activity of SOD, APX, CAT, and POD in its shoots and roots than Northstar. These results seem to indicate that Xinmu No. 1 cultivar's tolerance to salt or drought stresses during germination is associated with enhanced activity of antioxidant enzymes. This study highlights the importance of antioxidant enzymes in the establishment of alfalfa seedlings under drought and salinity conditions typical of desertification. 相似文献
16.
SNP和cPTIO对NaCl胁迫下拟南芥的生理影响 总被引:1,自引:0,他引:1
以拟南芥为材料,研究了0.5mmol/L的外源一氧化氮供体硝普纳(SNP)和200mmol/L的一氧化氮专一清除剂(cPTIO)对200mmol/LNaCl胁迫下对拟南芥的生理影响。0.5mmol/LSNP和200mmol/LcPTIO预处理2h后,加入200mmol/LNaCl。不同处理的拟南芥加入200mmol/LNaCl后,每隔12h取样一次,观察叶片生长情况、测定过氧化物酶(POD)、超氧化物歧化酶(SOD)、丙二醛(MDA)和超氧阴离子(O■)的变化。结果表明0.5mmol/L的SNP能缓解200mmol/LNaCl胁迫伤害,促进盐胁迫下幼苗的生长,显著提高抗氧化酶系统中POD和SOD活性,显著降低MDA和O■的含量,从而提高植株的抗盐性,而NO专一清除剂cPTIO能逆转SNP的上述效应。 相似文献
17.
Transgenic tobacco plants overexpressing cotton glutathione S-transferase (GST) show enhanced resistance to methyl viologen 总被引:6,自引:0,他引:6
A GST (EC 2.5.1.18) gene (Gst-cr 1) from cotton was introduced into Nicotiana tabacum by Agrobacterium tumefaciens-mediated transformation. Transgenic tobacco plants overexpressing Gst-cr1 were normal in growth and mature compared with control, but had much higher levels of GST and GPx activities and showed an enhanced resistance to oxidative stress induced by a low concentration of methyl viologen (MV). Six antioxidant enzymes, glutathione S-transferase, glutathione peroxidase (EC 1.11.1.9), superoxide dismutase (EC 1.15.1.1), peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6), and ascorbate peroxidase (EC 1.11.1.11) were monitored in transgenic lines and non-transgenic control during MV treatments. When they were treated with 0.03 mmol/L of MV, both transgenic lines and control showed a rapid increase in the activities of GST, GPx, SOD, POD, APx, while the activity of CAT seemed to be irregular. The percent of the increase in SOD and POD activities was much higher in control than in transgenic plants. When treated with 0.05 mmol/L of MV, both control and transgenic plants were severely damaged, and the activities of the six enzymes decreased sharply. 相似文献
18.
Tihana Marček Mirta Tkalec Željka Vidaković-Cifrek Marin Ježić Mirna Ćurković-Perica 《Acta Physiologiae Plantarum》2014,36(7):1739-1747
Salinity is an important abiotic factor that limits plant growth and development. The influence of salt stress induced by sodium chloride on plant growth, proline content, level of lipid peroxidation and activities of antioxidative enzymes was studied in F1 hybrid DH10 and four dihaploid lines (207B, 238C, 239K, 244B) of tobacco (Nicotiana tabacum L.). Dihaploids were obtained from anther-derived haploids of hybrid DH10 and were previously proved to be tolerant to Potato virus Y (PVY). In our study, plants were grown in vitro and exposed to NaCl (100 and 200 mM) for 33 days. All dihaploids and hybrid DH10 showed reduced growth after NaCl treatment. They accumulated significant amounts of sodium and proline in response to salt stress as have already been observed in tobacco and other plant species. In tobacco exposed to NaCl the lipid peroxidation level did not increase and activities of superoxide dismutase (SOD), guaiacol peroxidase (POD), ascorbate peroxidase and catalase (CAT) mostly did not change significantly. The exception was line 239K where salt induced higher activities of SOD, CAT and POD. Two (238C and 244B) out of four dihaploids appeared more susceptible to salt stress as they showed weak growth in correlation with high proline and sodium content. Therefore, it seems that salt tolerance is not associated with tolerance to PVY. Variations in malondialdehyde and proline content as well as in enzymes activities observed among tobacco lines imply that dihaploids have different genetic properties which might result in different sensitivity to NaCl. 相似文献
19.
水杨酸调节决明根系铝诱导的氧化胁迫 总被引:2,自引:0,他引:2
水杨酸(Salicylicacid,SA)在调节生物和非生物胁迫,诱导植物氧化胁迫中起着重要的作用,但对铝诱导的氧化胁迫的调节作用尚不清楚。本文研究了SA对决明(CassiatoraL.)根系铝诱导的H2O2和O2-含量变化,包括抗氧化酶活性以及细胞质膜过氧化胁迫变化的影响。介质中20mmol/L铝处理增加质膜透性,导致MDA含量上升及根尖细胞Evansblue染色加重(测定细胞死亡),而外源供给5mmol/LSA能缓解铝诱导的氧化胁迫。SA处理能明显降低根尖H2O2和O2-的含量,但两者含量与CAT、APX和GR的活性变化没有相关性,而与POD活性增加有关。水杨酸诱导H2O2含量的下降与抑制O2-积累和SOD活性有关。结果表明,SA可能激活一条由H2O2介导的、依赖于POD的抗氧化机制来缓解脂质的过氧化作用。 相似文献
20.
木薯华南8号组培苗对盐胁迫的生理响应 总被引:1,自引:0,他引:1
以NaCl胁迫生长的木薯(Manihot esculenta)华南8号(SC8)组培苗为材料,研究不同浓度(0、5、20、35、50 mmol·L-1及R50 mmol·L-1)NaCl处理对SC8组培苗的生长状况及叶绿素、过氧化氢(H2 O2)、丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性的影响.结果表明:≤20 mmol·L-1的NaCl胁迫60 d对SC8组培苗的生长基本无影响;≥35 mmol·L-1的NaCl胁迫60 d抑制了SC8组培苗的生长,但高浓度(50 mmol·L-1)胁迫30 d后正常培养30 d,可以使SC8组培苗的长势得到恢复.叶绿素和MDA含量在≤35 mmol·L-1 NaCl处理下较对照出现积累现象,随NaCl浓度升高(≥50 mmol·L-1)含量开始下降;与对照相比,H2 O2含量在NaCl胁迫下未出现积累现象.NaCl胁迫下,POD、CAT和APX活性较对照均有所提高;较高浓度的NaCl处理下,SOD、CAT和APX活性开始降低.实时荧光定量PCR结果表明,≥50 mmol·L-1NaCl胁迫下,SOD、CAT、POD和APX的表达水平较对照出现上升现象.这说明短时间的盐胁迫不会对木薯造成致死伤害,可以通过调节生理指标的活性来提高木薯的耐盐性. 相似文献