不同生长期白羊草中游离氨基酸的含量

本文对不同生长期白羊草（Ｂｏｔｈｒｉｏｃｈｌｏａｉｓｃｈａｅｍｕｍ）中氨基酸成分进行了分析,共检出白羊草中氨基酸１６种,以８月份生长的白羊草氨基酸的含量最高,为５．８１６ｍｇ／ｇ。     

聚球藻类金属硫蛋白的纯化及部分性质的研究

单细胞蓝藻（Ｓｙｎｅｃｈｏｃｏｃｕｓｓｐ．ＰＣＣ７９４２）以５０μｍｏｌ／ＬＺｎ２＋诱导８ｄ后收集,破碎取上清液经凝胶过滤、离子交换层析及反相ＨＰＬＣ纯化得到类金属硫蛋白,产率为每升培养液收集１．５ｇ鲜藻,得２．５ｍｇ纯品．其单体分子量为８７５０,Ｎ未端测定为缬氨酸,氨基酸组成分析得每分子（５６个氨基酸）含１０个半胱氨酸,疏水氨酸较多,且含有芳香族氨基酸,原子吸收光谱测得每分子蛋白结合４个二价金属．以上表明,该种类金属硫蛋白与哺乳动物金属硫蛋白结构差异很大,可能只是一种进化上的趋同．     

广东眼镜蛇蛇胆的鉴别及质量控制的研究Ⅳ：广东眼镜蛇蛇胆中牛磺酸、甘氨酸、牛磺胆酸和甘氨胆酸的测定

本文采用日立８３５－５０型氨基酸自动分析仪测定比较了广东眼镜蛇蛇胆及牛、猪、兔、鸡胆中牛磺酸、甘氨酸和其他氨基酸的成分与含量,并间接测定了牛磺胆酸与甘氨胆酸的含量和比值。结果表明广东眼镜蛇蛇胆中牛磺酸（６．６６７ｇ／１００ｇ干重）与牛磺胆酸（２７．４７ｇ／１００ｇ干重）的含量最高,而甘氨酸（０．５９８ｇ／１００ｇ干重）与甘氨胆酸（３．７０ｇ／１００ｇ干重）的含量最低,其他氨基酸的含量甚微。实验结果为蛇胆的真伪鉴别及内在质量控制提供了有实用价值的科学依据,同时也提供了一个准确、灵敏、微量的测定方法。     

四川省凉山州北部栽培苦荞麦的遗传多样性研究

用等位酶电泳技术测定了四川省凉山彝族自治州北部越西和甘洛２县的苦荞麦「Ｆａｇｏｐｙｒｕｍ ｔａｔａｑｒｉｃｕｍ（Ｌ．）Ｇａｅｒｔｎ．」１７个栽培居群的遗传多样性和分化,结合农业生物学性状进行了分析。对７个酶系统的１５个位点的检测表明,苦荞麦居群内的遗传多样性水平高于该州南部的苦荞麦和其他地区甜荞麦,每个位点的等位基因平均数为１．９,多态位点比率为５２．１％,平均实际杂合度和预期杂合度分别为０．１９     

马占相思(Acacia mangium)的生长、固氮及叶状柄的营养成分

马占相思（Ａｃａｉｃａ ｍａｎｇｉｕｍ）在厦门地区生长良好,生长速度和地上部重复一均大于肯氏相思（Ａ．ｃｕｎｎｉｎｇｈａｍｉａ）和大叶相思（Ａ．ａｕｒｉｃｕｌａｅｆｏｒｍｉｓ）。４年生的马占相和大叶相思人工林年凋落物的干重分别为１１５ｇ ｍ＾２、６６５８．ｇ ｍ＾２。马占相思和肯氏相思根瘤氮酶活性均有明显的季节变化,８份〉１０月分〉５月份〉２月分,马占相思叶状柄营养丰富,含有较高的蛋白质和氨基酸,     

苦瓜冻干粉对动物的降血糖作用及急性毒性

对药食同源植物苦瓜（Ｍｏｍｏｒｄｉｃａ ｃｈａｒａｎｔｉａ Ｌ．）的鲜汁冻干粉对动物的降血糖作用及急性毒性进行了药理实验,苦瓜冻干粉（生药量２０ｇ／ｋｇ和４０ｇ／ｋｇ）对药物性高血糖小鼠具有显著的降血糖作用,同时对正常小鼠血糖没有影响;苦瓜冻干粉的小鼠口服ＬＤ５０为生药量７０４．８ｇ／ｋｇ（９５％可信限生药量６１８．４～８０４ｇ／ｋｇ）,其产生降糖药效的剂量仅为其ＬＤ５０的１／３５～１／１７。     

考虑土壤水分限制的春小麦生长简化模型

建立了以日长（Ｌ）和日总辐射（ＴＤＲ）为自变量的春小麦（Ｔｒｉｔｉｃｕｍ ａｅｓｔｉｖｕｍ Ｌ．）碳同化和干物质累积（ＤＭＡ）的简化模型,考虑了水分限制下光能利用效率（ａ）,碳传输导度（ｇｃ）均被降低的设定,将反映春小麦水分状况的叶水热（Ψ）作为参数纳一模型,Ψ则通过多元回旭由８０ｃｍ土层相对含水量（θＲ,８０＿,气温（Ｔａ）水汽压差（ＶＰＤ）,低于－１．５ＭＰａ叶水势之和（Ψｃ,１．５）给出。讨     

喹诺酮类药物抗乙型肝炎病毒体外实验研究

本文以２．２．１５细胞株为模型,以ＨＢｓＡｇ、ＨＢｅＡｇ、ＨＢＶＤＮＡ、细胞存活率为观察指标,综合评价了喹诺酮类药物吡哌酸（ＰｉｐｅｍｉｄｉｃＡｃｉｄ）、氟哌酸（Ｎｏｒｆｌｏｘａｃｉｎ）、环丙氟哌酸（Ｃｉｐｒｏｆｌｏｓｘａｃｉｎ）、氟嗪酸（Ｏｆｌｏｘａｃｉｎ）体外抗ＨＢＶ效果。结果表明：吡哌酸、氟哌酸、环丙氟哌酸、氟嗪酸对ＨＢｓＡｇ、ＨＢｅＡｇ５０％抑制浓度（ＩＤ＿（５０））分别为１１μｇ／ｍｌ、６４μｇ／ｍｌ、９３μｇ／ｍｌ、１０５μｇ／ｍｌ和１９９μｇ／ｍｌ、１１１μｇ／ｍｌ、２４μｇ／ｍｌ、２１７μｇ／ｍｌ,细胞存活率为５０％时的药物浓度（ＣＤ＿（５０））分别为２１９μｇ／ｍｌ、９０μｇ／ｍｌ、１８１μｇ／ｍｌ、１６９μｇ／ｍｌ,在所选定的用药浓度范围内不同程度抑制培养上清液及细胞内ＨＢＶＤＮＡ及其复制中间体的产生。尤其对超螺旋结构ＤＮＡ（ｓｃＤＮＡ）有不完全抑制作用。     

中国河南株丁型肝炎病毒全基因组的cDNA克隆和序列分析

从我国河南－抗丁型肝炎病毒抗原（ａｎｔｉ－ＨＤＡｇ）及丁型肝炎病毒（ＨＤｖ）ＲＮＡ双阳性的ＨＢｓＡｇ携带者血清中提取ＲＮＡ,采用人工合成的引物进行逆转录和聚合酶链反应（ＰＣＲ）,获得了贯穿ＨＤＶ全基因组的６个相互重叠的ｃＤＮＡ片段。经双脱氧末端终止法进行核苷酸序列分析,得到了长度为１６７４ｂｐ的我国人河南株ＨＤＶｃＤＮＡ全序列。计算机分析表明,该株与我国台湾株（ＨＤＶＩＡ型）、美国－１株（ＨＤＶＩＢ型）、日本－１株（ＨＤＶⅡ型）和秘鲁－１株（ＨＤＶⅢ型）的核苷酸同源性分别为的９４．３％、８６．８％、７５．４％和６６．３％,氨基酸序列的同源性分别为８９．７％、８５．１％、７１．９％和６４．６％,并在核苷酸和推导的ＨＤＡｇ氨基酸序列中分别发现了５个和２个集中保守的区域。这些区域均与ＨＤＶ的某些重要功能密切相关。     

汉江中下游水生植物多样性及南水北调工程对其影响

１９９７—２０００年对汉江中下游８个江段水生植物种类组成、分布、生物量做了调查。汉江中下游共有水生植物１８科２２属３４种,以世界广布种为主,Ｒ／Ｔ值为０．６７。中游以沉水植物群落为优势,建群种为轮叶黑藻（Ｈｙｄｒｉｌｌａ　ｖｅｒｔｉｃｉｌｌａｔａ）、穿叶眼子菜（Ｐｏｔａｍｏｇｅｔｏｍ　ｐｅｒｆｏｌｉａｔｕｓ）和微齿服于荣（Ｐ．ｍａａｃｋｉａｎｕｓ）;下游主要为挺水植物群落,建群种为东方香蒲（Ｔｙｐｈａ　ｏｒｉｅｎｔａｌｉｓ）和芦苇（Ｐｈｒａｇｍｉｔｅｓ　ｃｏｍｍｕｎｉｓ）,生物量为５７．５—２１００ｇ／ｍ＾２;α－多样性在丹江口最高（０．５４６）,老河口次之（０．５１７）,仙桃员低（０）;生活型多样性在中游丹江口最大（０．４０２）;β—多样性在时间梯度６—８月份在所有样点β＝０,水平梯度β＝０．７９,垂直梯度β＝１．４８;γ—多样性在汉江中游为２９种,在下游为１０种。中游地区水生环境适合水生植物生长,而下游人类活动导致水环境趋于恶化限制了水生植物分布。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.