Contribution of natural food sources to reproductive behaviour, fecundity and longevity of Ceratitis cosyra, C. fasciventris and C. capitata (Diptera: Tephritidae)

The influence of food sources comprising the natural diet on the reproductive behaviour, fecundity and longevity of three African fruit flies Ceratitis cosyra (Walker), C. fasciventris (Bezzi) and C. capitata (Wiedemann) was investigated. Three natural food sources, varying in protein and sugar content, were evaluated. These included bird droppings (farm chicken), aphid honeydew and guava (Psidium guajava L.) juice. For C. fasciventris and C. capitata, flies fed on a protein-rich diet displayed higher frequency of calling, mating and oviposition than flies fed on a protein-poor diet, whilst for C. cosyra, quality of diet significantly influenced the mating behaviour of the flies, but not the calling and oviposition behaviour. Net fecundity rates were lowest for C. fasciventris and C. capitata when fed only on guava juice (0.1, 2.6 eggs per female, respectively), and higher for those on a diet of honeydew only (9.5, 33.8 eggs per female, respectively) and a combined diet of guava, honeydew and chicken faeces (11.8, 25.8 eggs per female, respectively). For C. cosyra, due to low numbers of eggs collected, no significant differences in fecundity between diets could be detected. All species fed only on a diet of chicken faeces since emergence died within the first three days of adult life without laying eggs, but when carbohydrates were provided by addition of guava juice and honeydew, the longevity of the flies was sustained for more than four weeks after adult emergence. The practical implications of these findings for control purposes are discussed.     

Effect of food deprivation on attractiveness of food sources, containing natural and artificial sugar and protein, to three African fruit flies: Ceratitis cosyra, Ceratitis fasciventris, and Ceratitis capitata

The effects of food deprivation, age, and mating status on the responses of three fruit fly species, Ceratitis cosyra (Walker), Ceratitits fasciventris (Bezzi), and Ceratitits capitata (Wiedemann) (Diptera: Tephritidae) to natural and artificial sugar and protein food sources were investigated. Natural food sources included guava [Psidium guajava L. (Myrtaceae)] juice (a common host fruit for all three fruit fly species) and bird faeces (farm chicken). Artificial food sources included molasses (obtained from a local sugar factory) and a locally produced protein bait (the International Centre of Insect Physiology and Ecology yeast). In all species studied, sugar deprivation of immature (1–2‐day‐old) male and female flies increased their response to food odours, although it did not change their preference for the type of odour (protein or sugar). Protein deprivation of mature (14–17‐day‐old) male and female flies also increased their response to food odours compared to protein‐fed flies. Protein‐deprived females were highly attracted to odours from protein sources in particular. Odours from natural food sources, guava juice, and chicken faeces, were more attractive to food‐deprived flies than were odours from artificial sugar and protein sources. Attraction to food odours increased significantly with increasing age for protein‐deprived females of all species. For males and females of all species, nutritional state was a more important factor than mating status in influencing responses of flies to food odours. Practical implications of these findings are discussed in terms of strategies for fruit fly control using food baits.     

Food sources for adult Diachasmimorpha longicaudata, a parasitoid of tephritid fruit flies: effects on longevity and fecundity

We report the results of a study on potential food sources of the widely distributed Indo‐Australian braconid fruit fly parasitoid Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae). Adults sustained life on diets of fruit juice or fruit pulp, a homopteran and its associated honeydew, or extrafloral nectary secretions. Longevities on all these foods and fecundity on fruit juice were comparable to those achieved on the honey that is typically provided in mass‐rearing programs. Certain of the flower species Bidens alba (L.), Spermacoce verticillata L., Lobularia maritima (L.) Desv., Brassica nigra (L.), Lantana camara L., their nectar or pollen, provided a diet that resulted in longer maximum life spans than water alone. Unlike some tephritid flies, the braconid did not feed on fresh bird feces or leaf‐surface exudates. Feeding by D. longicaudata on wounded host fruits of tephritid flies suggests that adult parasitoids would not need separate forays for adult food and oviposition sites, as these occur in the same locations. We conclude that an inventory of adult foods may help target inundative releases of D. longicaudata and lead to improvements in diets used for mass rearing.     

Sexual development of wild and mass‐reared male Queensland fruit flies in response to natural food sources

Diet has a profound influence on the fitness of adult tephritid flies. Mass‐reared flies are provided yeast hydrolysate as a rich source of nutrition that supports rapid sexual development and mating success. In contrast, wild tephritid flies often live in environments where food may be hard to find, and these are the conditions that sexually immature mass‐reared sterile males encounter when released into the field during sterile insect technique campaigns. The effect of natural food sources (bat guano, bird droppings, citrus pollen, and wheat pollen) on the sexual development of adult mass‐reared fertile, mass‐reared sterile, and wild male Queensland fruit flies, Bactrocera tryoni (Froggatt) (Diptera: Tephritidae), was determined by measuring ejaculatory apodeme size. Inclusion of yeast hydrolysate in the adult diet was associated with faster growth of the ejaculatory apodeme in comparison with all other diets. Effects of diet were far less pronounced in mass‐reared males, which may indicate reduced nutritional requirements, whereas the ejaculatory apodeme of wild males fed on natural sources of food or sucrose alone did not increase in size over the first 20 days of adult life.     

The effects of geographic origin and antibiotic treatment on the gut symbiotic communities of Bactrocera oleae populations

The olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), is the major insect pest of olive orchards (Olea europaea L.), causing extensive damages on cultivated olive crops worldwide. Due to its economic importance, it has been the target species for a variety of population control approaches including the sterile insect technique (SIT). However, the inefficiency of the current mass‐rearing techniques impedes the successful application of area‐wide integrated pest management programs with an SIT component. It has been shown that insect mass rearing and quality of sterile insects can be improved by the manipulation of the insect gut microbiota and probiotic applications. In order to exploit the gut bacteria, it is important to investigate the structure of the gut microbial community. In the current study, we characterized the gut bacterial profile of two wild olive fruit fly populations introduced in laboratory conditions using next generation sequencing of two regions of the 16S rRNA gene. We compared the microbiota profiles regarding the geographic origin of the samples. Additionally, we investigated potential changes in the gut bacteria community before and after the first exposure of the wild adult flies to artificial adult diet with and without antibiotics. Various genera – such as Erwinia, Providencia, Enterobacter, and Klebsiella – were detected for the first time in B. oleae. The most dominant species was Candidatus Erwinia dacicola Capuzzo et al. and it was not affected by the antibiotics in the artificial adult diet used in the first generation of laboratory rearing. Geographic origin affected the overall structure of the gut community of the olive fruit fly, but antibiotic treatment in the first generation did not significantly alter the gut microbiota community.     

Sex differences in movement between natural feeding and mating sites and tradeoffs between food consumption,mating success and predator evasion in Mediterranean fruit flies (Diptera: Tephritidae)

Summary Systematic quantitative observations of the location and diel pattern of adult Mediterranean fruit fly, Ceratitis capitata (Wiedemann), activities were carried out in an orange grove and surroundings on the island of Chios in Greece. Natural fly foods were assessed for their contribution to fly longevity, fecundity and fertility. There were diel shifts in male and female location. Females required a substantial and varied diet to realize peak fecundity. This diet was acquired away from the primary host, orange. Foraging for food throughout most of the day on fig and non-host foliage (including feeding on bird droppings) as well as on fig fruit and grapes, females dispersed and fed more than males. A diet of grapes alone did not support any fecundity, contributing only to longevity. A diet of figs alone, on the other hand, sustained both longevity and egg production. Bird feces alone supported neither egg production nor longevity. However, when added to a diet of figs, bird feces significantly increased fly fecundity. Throughout most of the day, males aggregated in leks within the inner canopy of the primary host, orange. The arrival here during the warmest hours of the day of receptive females, followed by pair formation, reinforced the lek mating system on host foliage. In the afternoon, females shifted to orange fruit where they suffered from high predation mortality while ovipositing. Soon after, males also shifted to orange fruit, where they attempted matings with non-receptive ovipositing females. Male feeding on fig fruit occurred late in the day, a time when they were least likely to find a mate. Male survival did not differ between the natural diets. Tradeoffs between food consumption, mating success and predator evasion are discussed for each sex and related to fruit fly mating systems.     

House Fly (Musca domestica L.) Attraction to Insect Honeydew

House flies are of major concern as vectors of food-borne pathogens to food crops. House flies are common pests on cattle feedlots and dairies, where they develop in and feed on animal waste. By contacting animal waste, house flies can acquire human pathogenic bacteria such as Escherichia coli and Salmonella spp., in addition to other bacteria, viruses, or parasites that may infect humans and animals. The subsequent dispersal of house flies from animal facilities to nearby agricultural fields containing food crops may lead to pre-harvest food contamination with these pathogens. We hypothesized that odors from honeydew, the sugary excreta produced by sucking insects feeding on crops, or molds and fungi growing on honeydew, may attract house flies, thereby increasing the risk of food crop contamination. House fly attraction to honeydew-contaminated plant material was evaluated using a laboratory bioassay. House flies were attracted to the following plant-pest-honeydew combinations: citrus mealybug on squash fruit, pea aphid on faba bean plants, whitefly on navel orange and grapefruit leaves, and combined citrus mealybug and cottony cushion scale on mandarin orange leaves. House flies were not attracted to field-collected samples of lerp psyllids on eucalyptus plants or aphids on crepe myrtle leaves. Fungi associated with field-collected honeydews were isolated and identified for further study as possible emitters of volatiles attractive to house flies. Two fungal species, Aureobasidium pullulans and Cladosporium cladosporioides, were repeatedly isolated from field-collected honeydew samples. Both fungal species were grown in potato dextrose enrichment broth and house fly attraction to volatiles from these fungal cultures was evaluated. House flies were attracted to odors from A. pullulans cultures but not to those of C. cladosporioides. Identification of specific honeydew odors that are attractive to house flies could be valuable for the development of improved house fly baits for management of this pest species.     

Potential for host range expansion in Ceratitis capitata flies: impact of proximity of adult food to egg-laying sites

Abstract.

• 1 In field cage and field tests, female Mediterranean fruit flies, Ceratitis capitata (Wiedemann), in trees alighted in significantly greater numbers upon sticky-coated (non-odour-emitting) kumquats, Fortunella japonica, that were in the vicinity of odorous natural proteinaceous food (bird faeces) or synthetic food odour than on similar kumquats distant from bird faeces or synthetic food odour.
• 2 In field cage tests, oviposition in non-sticky kumquats nearby bird faeces was significantly greater than in non-sticky kumquats distant from bird faeces.
• 3 In field tests, medflies laid significantly more eggs in host kumquat and non-host hawthorn, Crataegus mollis, fruit adjacent to bird faeces and synthetic food odour than in fruit of these types distant from food-type stimuli.
• 4 These findings suggest that odour of natural food of medflies could lure flies to plants whose fruit emit little or no attractive odour and are not permanent hosts but which are nonetheless susceptible to egg-laying and larval development, resulting in temporary expansion of host range.

     

Effect of plant oils on quality parameters of Bactrocera tryoni (Froggatt) reared on liquid larval diet

Liquid larval diets have been developed for several tephritid fruit flies including Queensland fruit fly, Bactrocera tryoni (Frogatt) (Q‐fly). In liquid diets, wheat germ oil (WGO) is usually added to improve performance in some quality parameters of reared flies, especially flight ability. However, for some flies, other plant oils may be more readily available, cheaper or produce flies of superior performance. In the present study, four alternative types of plant oils – rice bran, canola, vegetable, and sesame – were incorporated into a fruit fly liquid larval diet to replace the currently used wheat germ oil and their efficacy on the quality parameters of reared Q‐fly was compared to diets containing wheat germ oil or no oil. The quality parameters included: total pupal yield (N), pupal recovery (%), larval duration (days), pupal weight (mg), adult emergence (%), adult fliers (%), rate of fliers (%), sex ratio (%), F₁ egg/female/day and egg hatching (%). There were significant differences among treatments in performance of Q‐fly. Vegetable oil appeared better in terms of total pupal yield, percentage of pupal recovery, percentage of adult emergence, percentage of fliers, mean egg/female/day and % F₁ egg hatch compared with other oil treatments, especially from that of WGO treated diet. The result suggests that WGO can be substituted with rice bran and vegetable oil to improve the liquid larval diet for rearing of B. tryoni, with vegetable oil being the best replacement.     

The effect of developmental nutrition on life span and fecundity depends on the adult reproductive environment in Drosophila melanogaster

Both developmental nutrition and adult nutrition affect life‐history traits; however, little is known about whether the effect of developmental nutrition depends on the adult environment experienced. We used the fruit fly to determine whether life‐history traits, particularly life span and fecundity, are affected by developmental nutrition, and whether this depends on the extent to which the adult environment allows females to realize their full reproductive potential. We raised flies on three different developmental food levels containing increasing amounts of yeast and sugar: poor, control, and rich. We found that development on poor or rich larval food resulted in several life‐history phenotypes indicative of suboptimal conditions, including increased developmental time, and, for poor food, decreased adult weight. However, development on poor larval food actually increased adult virgin life span. In addition, we manipulated the reproductive potential of the adult environment by adding yeast or yeast and a male. This manipulation interacted with larval food to determine adult fecundity. Specifically, under two adult conditions, flies raised on poor larval food had higher reproduction at certain ages – when singly mated this occurred early in life and when continuously mated with yeast this occurred during midlife. We show that poor larval food is not necessarily detrimental to key adult life‐history traits, but does exert an adult environment‐dependent effect, especially by affecting virgin life span and altering adult patterns of reproductive investment. Our findings are relevant because (1) they may explain differences between published studies on nutritional effects on life‐history traits; (2) they indicate that optimal nutritional conditions are likely to be different for larvae and adults, potentially reflecting evolutionary history; and (3) they urge for the incorporation of developmental nutritional conditions into the central life‐history concept of resource acquisition and allocation.     

Enterobacter sp. AA26 gut symbiont as a protein source for Mediterranean fruit fly mass-rearing and sterile insect technique applications

Background

The interaction between gut bacterial symbionts and Tephritidae became the focus of several studies that showed that bacteria contributed to the nutritional status and the reproductive potential of its fruit fly hosts. Anastrepha fraterculus is an economically important fruit pest in South America. This pest is currently controlled by insecticides, which prompt the development of environmentally friendly methods such as the sterile insect technique (SIT). For SIT to be effective, a deep understanding of the biology and sexual behavior of the target species is needed. Although many studies have contributed in this direction, little is known about the composition and role of A. fraterculus symbiotic bacteria. In this study we tested the hypothesis that gut bacteria contribute to nutritional status and reproductive success of A. fraterculus males.

Results

AB affected the bacterial community of the digestive tract of A. fraterculus, in particular bacteria belonging to the Enterobacteriaceae family, which was the dominant bacterial group in the control flies (i.e., non-treated with AB). AB negatively affected parameters directly related to the mating success of laboratory males and their nutritional status. AB also affected males’ survival under starvation conditions. The effect of AB on the behaviour and nutritional status of the males depended on two additional factors: the origin of the males and the presence of a proteinaceous source in the diet.

Conclusions

Our results suggest that A. fraterculus males gut contain symbiotic organisms that are able to exert a positive contribution on A. fraterculus males’ fitness, although the physiological mechanisms still need further studies.

     

Feeding substrates and behaviors of Western cherry fruit fly (Diptera: Tephritidae)

A study was conducted to determine the abundance of potential foods and the feeding substrates and behaviors of the western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), in 2005, 2006, and 2007 in central Washington state. Aphid colonies with honeydew, a presumed food source for flies, were not seen on randomly selected branches of sweet cherry trees, Prunus avium L., but leaves with cherry juice, fruit that were damaged, and leaves with bird feces were commonly seen, especially later in the season. Grazing, a behavior in which the mouthparts rapidly move up and down and touch plant surfaces without discrete substances visible to the human eye, was seen more frequently in flies on leaves than on fruit. Grazing occurred more frequently than feeding on extrafloral nectaries (EFNs) on leaf petioles, cherry juice on leaves, and bird feces on leaves. The percentages of females and males that grazed on leaves were not different in 2 of 3 yr, but the percentage of females that grazed was higher in a third year. Percentages of female and male flies that fed on EFNs, cherry juice, and bird feces did not differ. More flies grazed the tops than bottoms of leaves. Flies also grazed on leaves of apple, pear, and grape. The results support the hypotheses that R. indifferens feeds mostly on leaves rather than fruit and that leaf surfaces may be the main feeding substrates for R. indifferens throughout the season.     

Biological and Behavioral Effects of Heavy Metals in Drosophila melanogaster Adults and Larvae

Heavy metals are essential components of biological systems but are extremely toxic at high doses. As a result, we hypothesized that perception of heavy metals through gustation may exist in Drosophila melanogaster. In this study, we investigated the behavioral effects of iron, copper, zinc, and cadmium on D. melanogaster gustation, oviposition, and pupation-site selection. In addition, we examined the biological effects of heavy metals on the fruit fly survival and reproductive success. Our results illustrate that D. melanogaster responds behaviorally to the presence of high concentrations of heavy metals in food. All metals acted as repellents to the fruit flies at high doses, with the egg-laying and feeding of the female flies significantly decreasing. Furthermore, supplementation of heavy metals in the culture medium reduced survival to the adult stage and shortened the life span of adult flies. From these observations, we speculate that D. melanogaster avoidance behavior towards high concentrations of heavy metals may have a positive effect on their survival and reproductive success in nature, particularly in the presence of metal-contaminated food sources.     

Long Term Feeding Patterns Highlight Preference for Sucrose in the Fruit Fly <Emphasis Type="Italic">Anastrepha serpentina</Emphasis> When Given a Choice over Other More Nutritious Food Sources

Insects are known to be able to regulate food ingestion according to its nutrient composition rather than its energetic content. Several studies have found that individuals can feed selectively when given the opportunity, and balance the intake of different nutrients so as to optimize fitness. However, there are cases in which individuals do not strictly adopt this pattern of optimal nutrient balancing. This study examined the periodicity of feeding on different food substrates and also water by adult individuals of Anastrepha serpentina (Wiedemann) (Diptera: Tephritidae) over a 38-day observation period analyzing daily feeding patterns, feeding time, and sequences of feeding events. Two artificial food substrates, one rich in protein (hydrolyzed yeast) and one rich in carbohydrates (sucrose), and one natural diet (open sapodilla fruit) were offered alone or paired (sucrose vs. open fruit or sucrose vs. yeast) to newly emerged individuals during 38 days or until death. Water was also provided in all cases. Our results provide new and interesting insights on long-term individual dietary preferences and feeding periodicity in a tropical fruit fly, including water consumption. In general, flies exhibited different feeding patterns according to the diet, but preferred to feed on sucrose when offered a choice of sucrose and yeast. This preference was evident from the outset of the trial. When sucrose was offered together with an open fruit, the latter was preferred. However, there were conspicuous individual differences in temporal feeding patterns among flies fed the same diet, including variability in the number of consecutive days some individuals did not consume any food or water (up to five days in some cases). Individuals fed with sucrose only exhibited the lowest survival and those fed with sucrose-open fruit the highest. We discuss these feeding choices and their possible causes, including individual differences in the gut microbiota and nutritional reserves of newly emerged flies.     

The ingestion in wolf spiders I. Capacity of gut ofLycosa pseudoannulata

An wolf spider, Lycosa pseudoannulata, collected from the field were reared in test tubes supplied with fruit flies as the food, and the number and weight of fruit flies killed per unit time and the weight of residuum of killed flies were recorded. When the cumulative number of flies killed by a spider was plotted against time after the beginning of food supply, the angle of the curve obtained decreased gradually until a constant value. In this time, it seems that the gut of spider had saturated with food and the increase rate of number of flies killed reflects the rate of food disappeared from gut by assimilation and egestion. The amount of ingestion was obtained by subtracting the weight of residuum and the gain of live weight of the spider from the weight of killed flies. After the gut has saturated, the amount of food remained in the gut is considered to be equal to the capacity of gut, which is an essential factor in the study of predation. The amount of food disappearance from gut was also estimated. There was a linear relationship between the log body weight of spiders and the log capacity of gut. The linear relationship was also seen between the log total amount of ingestion and the log total amount of food disappeared from gut.     

Microbial mediation of fruit fly–host plant interactions: is the host plant the “centre of activity”?

Insects utilize resources in their environment with the aid of mutualistic or symbiotic mediation by microorganisms. Some insect species such as ants and termites often have complex ecological and evolutionary associations with their symbionts, while the nature and functional significance of such associations in non-social insects is often unclear. In the Dacinae (Diptera: Tephritidae), specific Enterobacteriaceae ( Erwinia herbicola , Enterobacter cloacae , Klebsiella oxytoca ) are believed to mediate interactions between the adult fruit flies and the larval host plant. This bacterial mediation is hypothesized as being integral to the larval host plant being the "centre of activity" of the fly. Using a non-pest, monophagous fruit fly ( Bactrocera cacuminata [Hering]), we tested this hypothesis by manipulating the fruiting state of its larval host plant ( Solanum mauritianum Scopoli) and subsequently assessing insect behaviour and phylloplane microflora on those hosts. On host plants that had never fruited, few flies or bacterial colonies were recorded, consistent with hypothesis expectations. On fruiting host plants or plants that had had their fruit removed, bacterial colonies were present; again consistent with expectation. However, few flies were recorded on fruit-removed plants and all fly behaviours, other than resting or oviposition, were rare or absent on any hosts; inconsistent with expectation. The general pattern of results suggested that female flies coming to oviposit on fruiting hosts were spreading Enterobacteriaceae, but such spread was incidental and not part of some mutualistic interaction between fruit flies and bacteria.     

Carbohydrate utilization by normal and γ-sterilized Dacus oleae

Non-sterilized adult olive fruit flies were able to survive and reproduce on mannose, glucose, fructose, sucrose, melibiose, trehalose, maltose, melezitose, and sorbitol, out of a series of 23 carbohydrates γ-sterilized flies were able to utilize the same carbohydrates with the exception of melezitose, indicating that sterilization with a 10 Krad dose did not affect their ability to utilize various carbohydrates. Since these carbohydrates are common constituents of the fly's natural food sources, the released sterile flies should be able to survive satisfactorily in the field. The possible presence of three carbohydrases is discussed.     

Dietary wheat germ oil and age influences fatty acid compositions in adult oriental fruit flies

The oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is a serious pest of fruit crops in Asia, several Pacific Islands and sometimes the western United States, particularly California. Sterile insect technique programs have been developed for management of several tephritid fruit fly pests. These programs are based on continuous production of adult fruit flies. The high expense of mass-rearing oriental fruit flies drives research to improve the cost effectiveness of rearing programs. One recent improvement for mass rearing oriental fruit flies involves adding wheat germ oil (WGO) to the larval culture medium, which improved several parameters of biological performance. The performance enhancing influence of WGO is due to the presence of polyunsaturated fatty acids (PUFAs), some of which are nutritionally essential for many insect species. We considered the issue of whether WGO supplementation of the larval culture medium influences the fatty acid make up of adult tissues. We report that WGO supplementation led to substantial increases in adult tissue C18 PUFAs. Unlike the outcomes of unrelated nutritional studies on moths, the PUFA components of WGO did not improve adult fruit fly performance. Taken with recent publications reporting that WGO in larval diets influences gene expression, we conclude that dietary WGO improves biological performance of adults through changes in tissue C18 PUFAs and gene expression.     

Nutritional status and the foraging behaviour of Bactrocera tryoni with particular reference to protein bait spray

Poisoned protein baits comprise a recognized method for controlling tephritid fruit flies in the form of a ‘lure‐and‐kill’ technique. However, little is known about how a fly's internal protein and carbohydrate levels (i.e. nutritional status) might influence the efficacy of this control. In the present study, the relationships between the internal levels of protein (as measured by total body nitrogen) and carbohydrate (as measured by total body carbon) of the fruit fly Bactrocera tryoni (Froggatt) (Diptera: Tephritidae) are investigated, as well as its foraging behaviours in response to protein, fruit and cue‐lure (a male‐specific attractant) baits. Small cage behavioural experiments are conducted using flies from cultures of different nutritional status and wild flies sampled from the field during the fruiting cycle of a guava crop. For female flies, increasing total body nitrogen is correlated with decreased protein foraging and increased oviposition activity; increasing total body carbon levels generate the same behavioural changes except that the oviposition response is not significant. For males, there are no significant correlations between changes in total body nitrogen and total body carbon and protein or cue‐lure foraging. For wild flies from the guava orchard, almost all of them are sexually mature when entering the crop and, over the entire season, total body nitrogen and total body carbon levels are such that protein hunger is unlikely for most flies. The results infer strongly that the requirements of wild, sexually mature flies for protein are minimal and that flies can readily gain sufficient nutrients from wild sources for their physiological needs. The results offer a mechanistic explanation for the poor response of male and mature female fruit flies to protein bait spray.