外源供氮水平对大豆生物固氮效率的影响

采用稳定性同位素15N自然丰度(15N natural abundance)技术,以小麦为参照植物,研究了盆栽条件下,在外源供氮0、0.8、2.0、4.0 mmol·L-1水平下大豆的生物固氮百分率以及生物固氮数量对植物氮的贡献.结果显示:(1)0～2.0 mmol·L-1外源供氮可显著提高大豆的生物量和固氮百分率,且于2.0 mmol·L-1处理下地上生物量最高,达104 g·m-2,比CK增加了48%;(2)在0.8 mmol·L-1的供氮水平下大豆生物固氮量最高,为1.318 g·m-2,占大豆植株总吸氮量的70.4%,而在4.0 mmol·L-1供氮水平下生物固氮量仅占植株总吸氮量的44%;随供氮水平的升高,大豆生物固氮量占总吸氮量的比重下降,说明在高水平外源氮下,大豆生物固氮能力受到抑制;(3)大豆生物固氮百分率、固氮数量及吸氮数量与地上生物量间均呈显著正相关关系.结果表明,应用稳定性15N同位素技术可以定量大豆的生物固氮效率,根瘤菌接种配合低浓度外源氮有利于大豆生物固氮潜能的释放,对提高大豆产量、减少化肥投入有积极的指导意义.     

固定化培养中氮磷浓度对钝顶螺旋藻生长及其代谢产物和叶绿素荧光参数的影响

该研究以钝顶螺旋藻为实验对象,探究培养基中不同氮浓度(5、15、30、45 mmol·L^-1)和磷浓度(0.5、1.5、3.0、4.5 mmol·L^-1)对固定化生物膜培养模式下螺旋藻生长及其代谢产物和叶绿素荧光参数的影响,为生产实际中规模化高效培养螺旋藻提供理论依据。结果表明：(1)随着氮、磷浓度的升高,螺旋藻的生物量密度和产率先增高后降低,并在30 mmol·L^-1氮和3.0 mmol·L^-1磷处理时达到最大值;低氮、低磷条件会影响螺旋藻的藻体结构,表现为单细胞藻丝变短,螺旋变少,导致生物膜细胞密度降低。(2)与生物量变化趋势一致,螺旋藻光合色素含量随着培养基中氮、磷浓度的增加呈先升后降的变化趋势,且均在30 mmol·L^-1氮或者3.0 mmol·L^-1磷条件下达到最大值。(3)培养基中磷浓度固定时,螺旋藻的PSⅡ反应中心最大光能转化效率(F_v/F_m)和电子传递速率(ETR)随着氮浓度增加而增大,氮的缺乏会...     

pH、温度、NH4+和盐度对圆果雀稗内生固氮菌固氮特性的影响

采用乙炔还原法对从圆果雀稗(Paspalum orbiculare)根、茎中分离到的5株内生固氮菌(POJy11、POG12、POGy22、POGy612、POG4)的固氮特性进行了研究。结果表明,POGy612、POG4固氮的pH适应范围是6.0-10.0,其余3株菌为6.0-8.0;POGy22固氮的最适温度为35℃,其余4株菌为30℃;随着NH4 浓度和NaCl浓度的升高,菌株固氮活性总体呈下降趋势,其中POGy22的固氮活性受NH4 抑制最明显,NH4 浓度为1.0 mmol/L时,已无固氮活性,但POGy612在NH4 浓度为2.5 mmol/L时的固氮活性最高,达到1 045.0 nmol ml-1h-1,POJy11、POGy22在NaCl浓度为0.5%时的固氮活性最高,分别达到735.5 nmol ml-1h-1、703.0 nmol ml-1h-1。     

莲的离体快速繁殖技术

以莲(Nelumbo nucifera)授粉后18天的莲子胚芽为外植体,通过初代培养、继代培养和炼苗移栽,建立了莲离体快速繁殖体系。结果表明,将胚芽外植体诱导出无菌苗的最适初代培养基为MS固体培养基添加0.5 mg·L–1 6-BA、0.5 mg·L–1NAA、30 g·L–1蔗糖、0.5 g·L–1活性炭和0.8 g...     

不同培养条件对枝鞘藻生长、虾青素和油脂积累的影响

以丝状绿藻枝鞘藻(Oedocladium sp.)为实验材料,研究在100、300μmol·m-2·s-1和双侧300μmol·m-2·s-13种光强以及1、3、9、18 mmol/L 4种初始氮浓度下,两步法培养(第12 d时实验组分别更换为无氮培养基及加盐培养基)对枝鞘藻生长、油脂和虾青素积累的影响。结果显示:枝鞘藻最大生物量在双侧300μmol·m-2·s-1光强,18 mmol/L初始氮浓度更换为无氮培养基的条件下达到,为9.61 g/L;最高虾青素含量和最高油脂含量在双侧300μmol·m-2·s-1光强,3 mmol/L初始氮浓度更换为加盐培养基条件下达到,分别达到干重的1.62%和51.19%。研究结果表明高光条件有利于枝鞘藻的生长,双侧高光条件下低氮浓度更换为加盐培养基最有利于枝鞘藻虾青素和油脂的积累。     

Ca~(2+)和K~+对拟南芥幼苗镉毒害的缓解作用

该文探讨了外源钙(Ca)或钾(K)处理对不同程度的镉(Cd)胁迫(0–80μmol·L–1)下拟南芥(Arabidopsis thaliana)幼苗的生长和生理特性的影响。综合Ca和K对不同浓度Cd胁迫下拟南芥幼苗生长、根长以及生物量的影响情况,表明各浓度Cd胁迫下外源Ca2+的最适缓解浓度均为10 mmol·L–1;而K+的最适缓解浓度在低浓度(20和40μmol·L–1)和高浓度(60和80μmol·L–1)Cd胁迫下分别为10 mmol·L–1和20 mmol·L–1。在低浓度Cd胁迫下,添加适宜浓度的Ca2+或K+后幼苗可溶性蛋白和丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活性相比未添加Ca和K的对照组无显著变化,而过氧化物酶(POD)活性和总酚、类黄酮、花色素苷,酸溶性硫醇化合物、谷胱甘肽(GSH)、植物螯合肽(PCs)的含量均下降;高浓度Cd处理下,添加适宜浓度的Ca2+或K+后幼苗的SOD活性升高,POD活性降低,可溶性蛋白、MDA、总酚、类黄酮、花色素苷、酸溶性硫醇化合物、GSH以及PCs的含量也均低于对照组。在各浓度Cd胁迫下,添加外源Ca或K均使拟南芥幼苗根部细胞DNA损伤减弱,表现为TT嘧啶二聚体的累积量显著减少(P0.05)。以上结果表明,在Cd胁迫(尤其是高浓度Cd胁迫)下,外源Ca或K通过调节酚类、金属螯合物质的代谢水平以及提高拟南芥的抗氧化能力来缓解Cd对拟南芥幼苗的毒害效应,缓解细胞DNA损伤。该研究结果不仅能够为深入探讨Ca和K对缓解重金属毒害的分子机理提供实验依据,而且为Ca和K应用于重金属污染的防治提供参考。     

土壤杆菌固氮生理特性研究

对根癌土壤杆菌C58/pGV3850菌株的固氮生理特性研究结果表明,该菌具有自生固氮活性,其固氮活性的最适pH为8.0,温度为30℃.固氮活性在对数生长后期(14h)最高,延缓期和静止期乙炔还原活性较低.该菌株好氧,通过氧呼吸和吸氢酶的作用达到避氧固氮.当通入氧气超过呼吸耗氧时,对固氮活性产生抑制作用.在培养过程中增加NH_4~+浓度,固氮活性会降低,当达到15mmol/L时完全丧失固氮活性,表现NH_4~+阻遏固氮酶蛋白合成.在乙炔还原测定系统中加入NH_4~+并不影响乙炔还原反应,说明没有NH_4~+关闭现象.培养过程中加入MSX(2.5mg/ml)能解除10mmol/L NH_4~+对固氮酶合成的阻遏作用.固定的游离氮不能以NH_4~+形式分泌于胞外.固氮过程中放出大量氢气,培养16小时产氢量达65μmol H_2/mg蛋白.在限碳条件下(0.2%蔗糖)其吸氢酶活力可达520nmol H_2·ml~(-1)·h~(-1).     

转座子Tn916对固氮螺菌Azospirillum brasilense的接合诱变及氨分泌菌株的筛选

以携带质粒pAM120(Ap~r,Tc~r/Tn916)的大肠杆菌(E.coli CG120)为供体菌株,与受体菌巴西固氮螺菌(Azospirillum brasilense)采用滤膜接合法进行接合转移,在选择平板上得到具较高频率的接合子(10~(-5)/每个供体菌,选择四环素抗性)。从846株四环素抗性接合子中进一步用奈氏法筛选得到氨分泌突变株3株。在无氮培养基上,其氨分泌量可达7.5～14.0mmol/L。用乙炔还原法分析氨分泌突变株在不同浓度氮源上的固氮活力,发现20mmol/L NH Cl的存在不抑制其固氮活性,固氮活力与无氮条件下野生株的活力相差不多。无选择压力下细胞分裂50代后的稳定性实验证明,转座子Tn916在氨分泌突变株中的稳定性在50％～80％之间。以固氮螺菌氨分泌突变株为供体菌株,对E.coli HBX1进行反向接合转移实验,证实Tn916确实存在于氨分泌固氮螺菌接合子中。     

海洋酸化与磷浓度变化对龙须菜光合作用和ATPase活性的影响

以大型海藻龙须菜(Gracilaria lemanensis)为实验材料,设置不同CO2浓度(400μL·L–1和1000μL·L–1)和磷浓度(0.5和30μmol·L-1)实验,探讨大气CO2浓度升高对不同磷浓度培养下龙须菜生长、光合作用及ATPase活性的影响.结果显示大气CO2下,磷加富导致龙须菜的相对生长速率...     

苔藓-蓝藻共生体多样性及固氮潜力研究现状

苔藓与固氮蓝藻形成的共生体是许多受氮限制的天然陆地生态系统氮的主要来源,在全球氮循环中发挥着重要作用。不同生态系统的苔藓-蓝藻共生体物种组成及生长环境不同,固氮能力差异巨大。目前苔藓-蓝藻共生体的研究集中在北半球高纬度生态系统中,其他生态系统报道较少且零散。本文统计了已报道的苔藓-蓝藻共生体在全球生态系统中的分布、物种组成、蓝藻定殖率、蓝藻丰度及固氮潜力。统计发现,全球目前共发现参与苔藓-蓝藻共生的苔藓植物41科58属110种,蓝藻9科17属(≥26种);不同生态系统苔藓-蓝藻共生体的苔藓物种组成差异大,例如在北方森林中,赤茎藓(Pleuroziumschreberi)-蓝藻为优势共生体,泥炭藓(Sphagnumspp.)-蓝藻是湿地生态系统中的优势共生体,而念珠藻(Nostoc)类以其独特的生理特性和强大的生态适应能力成为多数生态系统中的优势蓝藻类群;不同生态系统中蓝藻在苔藓植物上的定殖率、丰度及固氮能力具有较大差异;在全球生态系统中,北极苔原及北方森林生态系统的固氮量均较高,最高的固氮量发生在北极苔原生态系统(1.3–24.6 kg N·ha^–1·yr     

一种高效研究大豆根瘤共生固氮的营养液栽培体系

为建立一种既可高效结瘤固氮, 又具有一定产量的大豆(Glycine max)营养液栽培系统, 设计并进行了2个试验。首先在不同供氮条件下, 研究了接种根瘤菌对大豆的结瘤状况、固氮能力、生物量及产量的影响。结果表明, 供氮过高或过低, 均影响大豆生长、产量形成及根瘤固氮; 并且植物生长所需的最适供氮水平远高于生物固氮所需的最适供氮水平。此外, 大豆生物固氮活性最高的时期在生殖期第一期(R1期)之前。由此推断, 大豆R1期前, 供应较低的氮, 有利于根瘤形成及固氮; 而从R1期起, 应提高供氮水平, 以促进植物生长及产量的形成。在此基础上开展第2个试验, 对供氮条件进行了优化处理(即R1期前低氮供应、R1期开始中氮供应)。结果表明, 与持续供应高氮相比, 优化供氮处理不仅可获得较多固氮酶活性较高的大根瘤, 还能保持较好的生长、获得更高的百粒重及维持80%左右的产量。研究结果不仅可为高效研究大豆根瘤共生固氮提供营养液配方, 还可为大豆高产高效栽培提供试验依据。     

Regulation of nitrogenase activity in Bradyrhizobium japonicum/soybean symbiosis by plant N status as determined by shoot C:N ratio

Regulation of nitrogenase is not sufficiently understood to engineer symbioses that achieve a high N₂ fixation rate under high levels of soil N. In the present hydroponic growth chamber study we evaluated the hypothesis that nitrogenase activity and the extent of its inhibition by NO₃⁻ may be related to both N and carbohydrate levels in plant tissues. A wide range of C:N ratios in various plant tissues (8.5 to 41.0, 1.9 to 3.7, and 0.8 to 1.8, respectively, in shoots, roots, and nodules) was generated through a combination of light and CO₂ levels, using two soybean genotypes differing in C and N acquisition rates. For both genotypes, N concentration in shoots was negatively correlated to nitrogenase activity and positively correlated to the extent of nitrogenase inhibition by NO₃⁻. Furthermore, nitrogenase activity was positively correlated to total nonstructural carbohydrates (TNC) and C:N ratio in shoot and nodules for both genotypes. Nitrogenase inhibition by NO₃⁻ was negatively correlated to TNC and C:N ratio in shoots, but not in nodules for both genotypes. At the onset of nitrogenase inhibition by NO₃⁻, C:N ratio declined in shoots but not in nodules. These results indicate that both C and N levels in plant tissues are involved in regulation of nitrogenase activity. We suggest that the level of nitrogenase activity may be determined by (1) N needs (as determined by shoot C:N) and (2) availability of carbohydrates in nodules. Modulation of the nitrogenase activity may occur through sensing changes in plant N, i.e. changes in shoot C:N ratio, possibly through some phloem translocatable compound(s).     

Regulation of nitrogenase activity in Bradyrhizobium japonicum/soybean symbiosis by plant N status as determined by shoot C:N ratio

Regulation of nitrogenase is not sufficiently understood to engineer symbioses that achieve a high N₂ fixation rate under high levels of soil N. In the present hydroponic growth chamber study we evaluated the hypothesis that nitrogenase activity and the extent of its inhibition by NO₃⁻ may be related to both N and carbohydrate levels in plant tissues. A wide range of C:N ratios in various plant tissues (8.5 to 41.0, 1.9 to 3.7, and 0.8 to 1.8, respectively, in shoots, roots, and nodules) was generated through a combination of light and CO₂ levels, using two soybean genotypes differing in C and N acquisition rates. For both genotypes, N concentration in shoots was negatively correlated to nitrogenase activity and positively correlated to the extent of nitrogenase inhibition by NO₃⁻. Furthermore, nitrogenase activity was positively correlated to total nonstructural carbohydrates (TNC) and C:N ratio in shoot and nodules for both genotypes. Nitrogenase inhibition by NO₃⁻ was negatively correlated to TNC and C:N ratio in shoots, but not in nodules for both genotypes. At the onset of nitrogenase inhibition by NO₃⁻, C:N ratio declined in shoots but not in nodules. These results indicate that both C and N levels in plant tissues are involved in regulation of nitrogenase activity. We suggest that the level of nitrogenase activity may be determined by (1) N needs (as determined by shoot C:N) and (2) availability of carbohydrates in nodules. Modulation of the nitrogenase activity may occur through sensing changes in plant N, i.e. changes in shoot C:N ratio, possibly through some phloem translocatable compound(s).     

基于等温滴定微量热技术的玉米脱落酸受体检测体系

脱落酸(ABA)是响应逆境胁迫及调控植物生长发育的重要激素,其受体的发现以及在不同植物中的比较研究具有重要的理论与实际意义。等温滴定微量热技术(ITC)是鉴定和筛选ABA受体的重要技术之一,该方法对受体蛋白的纯度和生物活性要求较高。该文探讨了超声波破碎条件对受体蛋白得率以及ABA和受体蛋白浓度对二者亲和力的影响。结果表明,通过超声波破碎获得的原核表达玉米(Zea mays)ABA受体蛋白Zm PYL1含量高,蛋白质图谱条带清晰。超声波破碎适宜的条件为:菌悬液浓度100 mg·m L–1,破碎总时长15分钟,单次破碎时长为3秒,间歇时长10秒;ITC检测结果发现,(±)-ABA与玉米受体Zm PYL1的结合反应为吸热过程,推测该受体蛋白Zm PYL1为二聚体,4 mmol·L–1(±)-ABA与0.1 mmol·L–1受体蛋白Zm PYL1反应结合效果较好,反应的解离常数Kd值为72.46μmol·L–1。研究结果为筛选和鉴定植物ABA受体奠定了重要技术基础。     

Interactive effects of phenolic acid and nitrogen on morphological traits of poplar (Populus × euramericana ‘Neva’) fine roots

Aims The relationship between rhizosphere process and fine root growth is very close but still obscure. In poplar plantation, phenolic acid rhizodeposition and soil nutrient availability were considered as two dominant factors of forest productivity decline. It is very hard to separate them in the field and they might show an interactive effect on fine root growth. The objective of this study is to examine the influence of phenolic acids and nitrogen on branch orders of poplar fine roots and to give a deeper insight into how the ecological process on root-soil interface affected fine root growth as well as plantation productivity. Methods The cuttings of health annual poplar seedlings (I-107, Populus × euramericana ‘Neva’) serve as experiment materials, and were cultivated under nine conditions, including three concentration of phenolic acids at 0X, 0.5X, 1.0X (here, X represented the contents of phenolic acids in the soil of poplar plantation) and three concentration of nitrogen at 0 mmol·L^-1, 10 mmol·L^-1, 20 mmol·L^-1, based on Hoagland solution. The roots were all separated from poplar seedlings after 35 days, and 30 percent of total fine roots of every treatment were taken as fine root samples. These fine roots were grouped according to 1 to 5 branch orders, and then the morphological traits of each group of fine roots were scanned via root analyzer system (WinRHIZO, Regent Instruments Company, Quebec, Canada) including total length, surface area, volume and average diameter. Meanwhile, the dry mass of fine root samples of every order was measured to calculate specific root length (SRL), root tissue density (RTD). All data were analyzed via SPSS 17.0 software, and interactive effect of phenolic acids and nitrogen on roots was analyzed through univariate process module. Principal component analysis (PCA) and redundancy analysis (RDA) were conducted via Canoco 4.5 software. Important findings Under the conditions without phenolic acids application, the fine roots growth was significantly inhibited in deficiency and higher nitrogen treatments, especially for 1-3 order roots. Only specific root length appeared decreased with nitrogen level, and other traits of fine roots did not demonstrate linear relationship with nitrogen concentrations. Compared to 0.5X phenolic acids treatment, 1.0X phenolic acids significantly promoted the diameter and volume of 1-2 order roots (p < 0.05). Both phenolic acids and nitrogen demonstrated influence on poplar fine root traits. However, the diameter and volume of 1-2 order roots were significantly affected by phenolic acids, while the total length and surface area of 4-5 order roots was affected by nitrogen. Two way ANOVA showed that phenolic acids and nitrogen made a synergistic or antagonistic effect on morphological building of fine roots. Furthermore, PCA and RDA indicated that the interactive effects of phenolic acids and nitrogen led to significant differences among 1-3 order, 4th order and 5th order of poplar fine roots. The PC1 explained about 60.9 percent of root morphological variance, which was related to foraging traits of roots. The PC2 explained 25.3 percent of variance, which was related to root building properties. The response of poplar roots to phenolic acids and nitrogen was closely related to root order, and nitrogen played more influence on poplar roots than phenolic acids. Thus, phenolic acids and nitrogen level would affect many properties of root morphology and foraging in rhizosphere soil of poplar plantation. But nitrogen availability would serve as a dominant factor influencing root growth, and soil nutrient management should be critical to productivity maintenance of poplar plantation.     

酚酸和氮素交互作用下欧美杨107细根形态特征

树木细根生长与根际过程的关系十分密切。该研究仿生欧美杨107 (Populus × euramericana ‘Neva’)人工林根际土壤酚酸沉降与氮素有效性变化, 通过设置3种酚酸梯度(0X、0.5X、1.0X, X为田间土壤酚酸含量)与3种氮素水平(缺氮0 mmol·L^-1、正常氮10 mmol·L^-1、高氮20 mmol·L^-1), 探究酚酸和氮素对欧美杨107细根形态的影响, 以期为阐明树木根系生长对根-土界面过程的响应奠定基础。结果表明: (1)在无酚酸(0X)环境中, 缺氮和高氮均可抑制欧美杨107细根生长, 尤其对1-3级细根的影响更为显著。比根长随氮素水平升高逐渐减小, 但其他细根特征并未呈现与氮素水平的线性关系。(2) 0.5X和1.0X酚酸梯度相比, 欧美杨107的1-2级细根直径和体积随酚酸浓度增加而显著增大(p < 0.05)。酚酸和氮素对杨树细根的影响存在交互作用, 1-2级细根直径、体积受酚酸的影响显著, 而4-5级细根长度、表面积受氮素影响显著。双因素方差分析结果表明, 酚酸和氮素对细根形态建成具有协同或拮抗效应。(3)主成分分析(PCA)和冗余分析(RDA)结果表明, 在酚酸和氮素交互效应下, 杨树1-3级、 4级、 5级细根之间具有显著的形态差异。第一主成分主要体现细根觅食性状特征, 可解释细根形态变异的60.9%的信息; 第二主成分主要体现细根形态构建特征, 可解释25.3%的信息。杨树细根形态变化与根序高度相关, N素影响杨树细根形态的主效应较酚酸更强。因此, 根际环境中酚酸累积和氮素有效性变化会影响杨树细根的形态构建和细根对水分、养分的吸收, 而氮素有效性是影响杨树细根生长的重要因素, 开展杨树人工林土壤养分管理是林分生产力长期维持的关键。     

氮、磷营养对雨生血球藻绿色细胞生长的影响

采用BBM培养基,以雨生血球藻(Haematococcus pluvialis CG-11)为研究材料,通过测定细胞生长速率、叶绿素和类胡萝卜素含量、生物量和虾青素含量,探讨氮、磷营养对雨生血球藻营养细胞生长的影响.结果显示:H.pluvialis CG-11生长的适宜氮源形式是NaNO₃和NH₄NO₃;适宜H.pluvialis CG-11生长的氮浓度为41.2mg·L^-1,磷浓度为5.3～53.3mg·L^-1.     

不同花生品种根瘤固氮特点及其与产量的关系

花生根系着生根瘤,能够直接利用大气中的氮气作为氮源,在花生氮素供应中占有举足轻重的地位.而有关根瘤高效固氮的机理研究甚少.本研究在盆栽条件下,利用¹⁵N示踪技术,研究了19个花生品种根瘤固氮特点及其与产量的关系.结果表明: 不同品种根瘤数量、鲜质量、内含物质和固氮量等指标品种间存在显著差异.根瘤数量和鲜质量变异幅度分别为每盆170.59～696.15个和0.83～3.74 g,变异系数分别为36.1%和41.1%;豆血红蛋白含量和固氮酶活性变异幅度分别为每盆15.51～23.23 mg和2.75～20.46 μmol C₂H₄·h^-1,变异系数分别为13.1%和57.2%,后者明显高于前者,表明固氮酶活性除受豆血红蛋白含量影响外,同时受到其他因素的影响.根瘤固氮和全氮积累量变异幅度分别为每盆0.71～1.82和2.16～3.72 g,变异系数分别为21.6%和12.9%,前者明显高于后者,表明花生根瘤固氮不足时,其他氮源在一定程度上能自动补偿根瘤留下的匮缺.花生以根瘤固氮为主,供氮比例平均占总氮量的2/5以上,最高可达50%,培育高供氮比例的品种,可作为花生减氮栽培的途径之一.上述指标中,除根瘤数量外,其余指标间以及这些指标与产量均呈极显著正相关,表明根瘤固氮生理指标与根瘤供氮能力及最终产量密切相关,提高这些指标有助于同时实现高产和化肥减施.     

不同初始氮浓度下尖状栅藻同化硝态氮和CO2的研究

尖状栅藻（Scenedesmus acuminatus（Lagerheim） Chodat）是一种高产油淡水单细胞绿藻,该藻在较低氮素浓度下能显著提高产油效率,是生产生物柴油的理想藻株。本研究以尖状栅藻为实验材料,通过测定藻细胞硝酸还原酶和Rubisco活性、碳氮元素含量和培养液硝酸根离子浓度,分析18.0、9.0、6.0、3.6 mmol·L^-1初始氮浓度下尖状栅藻的碳氮同化特征和时相变化规律。结果显示,18.0 mmol·L^-1组尖状栅藻细胞密度最高,为7.9×10⁷ cells·mL^-1,硝酸还原酶和Rubisco的活性高,且持续时间长。培养液中产生的NO₂^-随初始氮浓度的升高而增多,18.0 mmol·L^-1组至培养期结束仍保持相对较高的NO₂^-水平。4个实验组（初始氮从高到低）培养10 d藻细胞的氮含量依次为7.2%、4.1%、2.8%和1.9% DW,碳含量为46%、52%、54.5%和57.4% DW,细胞的C/N摩尔比值为：8.0、16.7、24.3和35.2。研究结果表明初始氮浓度影响尖状栅藻的生长繁殖,藻细胞的碳氮同化相互影响,适宜的低氮浓度可促进藻细胞碳固定。