共查询到20条相似文献,搜索用时 31 毫秒
1.
Aconitum vilmorinianum, a well-known traditional Chinese herb, is recently being threatened by overexploitation
and environment disturbance. This study was conducted to provide propagation methods through in vitro germination and explant cultivation. Germination was stimulated up to 66.00% on Murashige and Skoog (MS) medium containing 2.0 mg L−1 6-benzylaminopurine (BAP), 0.1 mg L−1 1-napthaleneacetic acid (NAA), and 30 g L−1
sucrose. Three bacteria (Pantoea agglomerans, Erwinia persicina, and Pseudomonas tolaasii) would be responsible
for consistent contamination during germination. The latter two were effectively eradicated after disinfected. The
influence of explant types and hormone combinations on direct and indirect organogenesis was evaluated in the
present work. The frequency of shoot induction from axillary bud explants was 100% on the MS fortified with
2.0 mg L−1 BAP and 0.3 mg L−1 NAA. Shoots multiplication was optimized on MS medium supplemented with
0.1 mg L−1 thidiazuron (TDZ) and 0.1 mg L−1 NAA. High callus induction percentage (96.67%) was obtained
from stem segments on MS medium with 2.0 mg L−1 2,4-D, then successfully regenerated into shoots on MS medium in the presence of 0.1 mg L−1 TDZ and 0.2 mg L−1 NAA. The present work could be useful for the utilization
and conservation of this valuable species. 相似文献
2.
Cinnamomum japonicum Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study,
we successfully developed a large-scale micropropagation protocol for C. japonicum for the first time. Sterilized shoots were excised and used as explants for
shoot induction on several basal media, supplemented with different concentrations
of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N6
-Benzyladenine
(6-benzylaminopurine) (BA), α-naphthaleneacetic acid (NAA) and Gibberellic acid
(GA3). After comparison, the most efficient medium for shoot regeneration
was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L–1 BA,
0.05 mg L–1 NAA and 0.2 mg L–1 GA3, which resulted in an average number of
induced shoots per explant and shoot length of 5.2 and 1.62 cm at 28 d, respectively. Then, elongated adventitious shoots were transferred to induce roots.
86.7% of shoots was able to root on 1/2 MS medium supplemented with 0.5 mg
L–1 NAA and 0.1 mg L–1 BA. The earliest rooting time observed was after 21 d
and the average root length was up to 3.3 cm after 28 d. Our study shows that
C. japonicum can be successfully regenerated through de novo organogenesis,
which lays a foundation for future transformation research on this tree. 相似文献
3.
An efficient regeneration system of Rhynchostylis retusa was established to provide technical reference for the
application of tissue culture tube seedlings in production. The mixtures of callus and protocorm from aseptic germination were used as explants. The optimal media of each stage was selected for callus proliferation, protocorm
occurrence and growth, rejuvenation and rooting via a single, complete combination and orthogonal experiment.
The results showed that the optimal medium for callus proliferation, protocorms occurrence and growth was 1/2
Murashige and Skoog (MS) medium adding 50 g·L−1 banana puree, 0.1 mg·L−1 α-naphthaleneacetic acid (NAA),
1.5 mg·L−1 6-benzylaminopurine (6-BA) and 1.0 mg·L−1 kinetin (KT) with 17.33 proliferation coefficient of callus
and 19.63 occurrence coefficient of buds after 90 days. Then the buds occurred from protocorm were cultured on
1/2 MS medium including 100 g·L−1 banana puree, 1.0 mg·L−1 NAA, 2.0 mg·L−1 6-BA and 0.05 mg·L−1 KT, in
which the proliferation coefficient of callus was 10.32 and occurrence coefficient of buds reached 17.87. In the
further subculture, the same medium was simultaneously used for callus proliferation, protocorm occurrence
and bud growth. The plantlets developed roots in 1/2 MS medium containing 70 mL·L−1 coconut water and
1.5 mg·L−1 NAA with 100% rooting rates after 90 days. The survival rate was more than 90% after domestication
and transplantation. This regeneration protocol will provide technique foundation for protecting wild resource
and developing artificial cultivation. 相似文献
4.
Lihui Zeng Haifeng Xu Yunqi Zeng Aiye Luan Huiquang Wang 《In vitro cellular & developmental biology. Plant》2009,45(5):559-564
Ponkan mandarin (Citrus reticulata Blanco) is one of the most important commercial cultivars of mandarin orange in China. This study reports an improved and
efficient protocol for in vitro plant regeneration of Ponkan mandarin. Epicotyl segments, which were cut longitudinally into two halves, were used as explants.
The shoot regeneration frequency was significantly increased by longitudinal cutting. A 100% shoot regeneration frequency
and 13.2 shoots per explant were obtained when cultures were maintained in darkness for 20 d before being transferred to light
conditions, with bud induction by indirect organogenesis. A 72.5% shoot regeneration frequency and 7.8 shoots per explant
were obtained when explants were incubated under a 16-h light photoperiod continuously with buds differentiating directly
from the cutting wound surface. The optimal medium for shoot formation was Murashige and Tucker basal medium supplemented
with 2 mgL−1 BA and 30 gL−1 sucrose both under light conditions. The addition of the auxin NAA reduced the frequency of regeneration. A “filter-paper
bridge” technique was used for rooting in this study. The basal portion of regenerated shoots was dipped into 1,000 mgL−1 IBA solution for 15 min before placement on a filter-paper bridge that was maintained in 1/2 MS liquid medium supplemented
with 10 gL−1 sucrose. Eighty percent of the shoots rooted, and an average of 2.0 roots per shoot were achieved. Survival rate through
acclimatization was 100%. 相似文献
5.
Summary A method of plant regeneration from hypocotyl segments of Platanus acerifolia Willd, has been developed. Hypocotyl slices were cultured on Murashige and Skoog (MS) basal medium supplemented with a range
of combinations of cytokinins [6-benzyladenine (BA) or kinetin] and auxins [indole-3-butyric acid (IBA), indole-3-acetic acid,
α-naphthaleneacetic acid or 2,4-dichlorophenoxyacetic acid] for adventitious shoot induetion. The highest regeneration frequency
was obtained with MS medium containing 2.0 mg l−1 (8.88 μM) BA and 0.5 mg l−1 (2.46 μM) IBA. Adventitious buds and shoots were differentiated from hypocotyl-derived cellus or directly from the wounded sites within
4–8 wk. The regenerated shoots were elongated and proliferated efficiently on multiplication medium. Complete plantlets were
transplanted to the soil and grew normally in the greenhouse after root formation on rooting medium for 4–6 wk. 相似文献
6.
An efficient regeneration method via shoot tip explant has been developed for Jatropha curcas, which is a medicinally as well as economically important plant. Shoot tips were proliferated on MS medium incorporated with BAP (2.0 mg l?1) and IAA (0.5 mg l?1) along with adenine sulphate, glutamine and activated charcoal. In vitro produced shoots were induced to root on IBA (0.5–5.0 mg l?1) added to half strength MS medium. The highest frequency of root induction was on the medium with 3.0 mg l?1 IBA. Regenerated plantlets were successfully transferred to field after initial acclimatization. 相似文献
7.
Mengtong Liu Yangyang Zhou Tongyu Liu Jianyu Lu Jing Qu Shuyan Guan Yiyong Ma 《Phyton》2022,91(2):363-374
Immature embryos of inbred maize (Zea mays) lines (H8183, H8184, and H8185) were used for Agrobacterium infection. We used the β-glucuronidase gene (GUS) as the target gene and the glufosinate resistance gene (bar) as the selection marker. We conducted research on several aspects, such as different genotypes, coculture conditions, screening agent concentrations, and concentrations of indole-3-butytric acid (IBA), 6-benzylaminopurine (6-BA), and ascorbic acid (Vc) in the differentiation medium. We optimized the genetic transformation system, and the obtained results indicated that among the three lines studied, the induction rate of H8185 was the highest at 93.2%, followed by H8184, with H8183 having the lowest induction rate (80.1%). The best coculture method was that using the N6 coculture medium layered with a sterile filter paper. Using orthogonal analysis, we found that the optimal combination of the three factors in the differentiation medium was A3 (1 mg mL−1 IBA), B3C1 (1.6 mg mL−1 6-BA), and D3 (1.5 mg mL−1 Vc). Through GUS staining analysis, Bar test-strip analysis, and polymerase chain reaction, five transgenic plants were finally obtained. This study established the optimal conditions for genetic transformation in maize. 相似文献
8.
Yaser Hassan Dewir Nisha Singh Shakira Shaik Ashley Nicholas 《In vitro cellular & developmental biology. Plant》2010,46(1):41-46
The present study reports a simple protocol for indirect shoot organogenesis and plant regeneration of Sutherlandia using rachis and stem segments. Different concentrations (0.0–68.08 μmol l−1) of thidiazuron (TDZ) were used for callus induction and shoot organogenesis. The highest percentage of callus formation
(97.5%) and the highest percentage of explants forming shoots (88.8%) were obtained from rachis explants cultured onto Murashige
and Skoog (MS) medium (Murashige and Skoog, Physiol. Plant. 15:473–495, 1962) supplemented with 45.41 μmol l−1 TDZ. Scanning electron microscopy demonstrated the early development of adventitious shoots derived from callus cultures.
Shoot clusters were further developed and grown in MS hormone-free medium. The presence of l-canavanine was determined by thin-layer chromatography and confirmed after column fractionation using silica gel and nuclear
magnetic resonance spectroscopy. Individual shoots were rooted on different concentrations and combinations of MS salt strength
and IBA. Half-strength MS salt medium supplemented with 24.6 μmol l−1 IBA was optimal for root induction in which 78% of shoots were rooted. The in vitro plants were successfully acclimatized in a growth chamber with a 90% survival rate. 相似文献
9.
Lucía Isabel Chávez-Ortiz José Francisco Morales-Domínguez Araceli Rodríguez-Sahagún Eugenio Pérez-Molphe-Balch 《Phyton》2021,90(3):1003-1013
Agave guiengolaGentry is an endemic plant from a very small locality in Oaxaca, Mexico. Its conservation status
is fragile and can rapidly worsen. Because of its scarcity, this agave has been used solely for ornamental purposes,
but it could have other uses if more plants were available. In vitro propagation by enhanced axillary sprouting
from stem segments was attained using Murashige and Skoog Basal Medium (MS) as well as basal medium supplemented with cytokinins 6-Benzylaminopurine (BA) or 6-(γ,γ-Dimethylallylamino)purine (2iP). The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l–1 BA, obtaining a mean
of 3.7 shoots per explant. Other interesting responses were observed, such as nodular callus induction using combinations of BA and 2,4-Dichlorophenoxyacetic acid (2,4-D); root induction without Plant Growth Regulators
(PGR); and generation of shoot clusters. These clusters constituted an excellent explant for micropropagation
in temporary immersion bioreactors, obtaining a propagation rate of 43 shoots per explant with 1 min immersion
and 6 h immersion frequencies. All new plants rooted and survived the transfer to soil. This study developed an in
vitro propagation scheme to produce individuals that can be used either for reforestation, economical purposes, or
to carry out studies in this species to assess its full potential, avoiding exploitation from wild plants. 相似文献
10.
Alfaro-Corres Arnoldo Enrique Lecona-Guzman Carlos Alberto Gutiérrez-Miceli Federico Antonio Martínez-Esteves Manuel Silverio-Gómez María del Carmen Medina-Lara María de Fátima Ruiz-Lau Nancy 《Phyton》2023,92(2):629-643
The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana L. In order to do the establishment of cells, the formation of callus was induced; for it two auxins were evaluated: 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-mino-3,5,6-trichloropicolinic acid (picloram) at three concentrations (0.25, 0.5 and 0.75 mg L−1) in three explants (leaf, root and meristems) cultured in MS semisolid medium. The callogenesis response was related to the type and section of the explant, as well as the regulator used, and a cell suspension was established using 0.5 mg L−1 naphthaleneacetic acid (NAA) + 0.5 mg L−1 Benzylaminopurine (BAP). Seedlings were exposed to polyethyleneglycol (15% and 30% w/v) with a water potential of −0.87 and −2.67 MPa, respectively, under soil conditions. Water stress was applied through restricted irrigation. Fresh weight, root system growth, and chlorophyll concentration were some of the parameters that were affected by the effect of water deficit on A. americana L. Chlorophyll concentration values were significantly decreased by 15 at 30% PEG (19.6 SPAD units) compared to the control treatment. In in vitro plants, the highest concentration of proline was found in the roots, being the treatment with 30% polyethylene glycol where the highest concentration of this osmoregulator was obtained (62.5 mg g−1 DW). Under restricted irrigation conditions, an increase in proline concentration was observed both in the aerial part (2.2 µg 100 g−1 DW) and in the root system (1.8 µg 100 g−1 DW). However, the concentrations found were approximately ten times greater, less than those found under in vitro conditions. Therefore, the accumulation of proline can be considered an indicator of stress in Agave Americana L. growth in vitro. 相似文献
11.
<i>In Vitro</i>-Propagation of <i>Agave tequilana</i> Weber cv. azul in a Temporary Immersion System
Otilio Vázquez-Martínez Héctor Gordon Núñez-Palenius Eugenio M. Pérez-Molphe Balch Mauricio Valencia-Posadas Luis Pérez-Moreno Graciela M. L. Ruiz-Aguilar M. Gómez-Lim 《Phyton》2022,91(1):83-96
In Mexico, there is a need to produce large quantities of plantlets for the establishment and replanting of blue (cv. azul) agave production areas. Most of these plots are within the origin denomination area (DOT, Spanish acronym) of the distilled product of this plant, known as tequila. The objective of this study was to develop an in vitro-propagation protocol for Agave tequilana Weber cv. azul using segmented stems in both: solid and liquid media. A disinfection and in vitro technique were developed to obtain shoots, through plantlets collected in commercial plots, which attained 100% surface-disinfection and budding rate. At the multiplication stage, the effects of 6-Benzylaminopurine (BA) (0.0, 4.4 and 13.2 μM) and kinetin (0.0, 9.4, 18.8 and 37.6 μM) were evaluated on lateral-shoot production of segmented sagittal stems. These were cultivated on Murashige & Skoog (MS) medium, with the addition of 3.0% sucrose and 8 g L−1 agar. It was observed that BA and kinetin increased the number of shoots per explant, obtaining up to 18 and 26, respectively. Furthermore, it was found that just the sagittal segmentation of explants increased axillary budding. On the other hand, segmented-stem bases were grown in MS liquid medium with 3.0% sucrose, inside a RITA® system, programmed by a 5 min immersion step with a frequency of every 4 h. The effect of Indole−3-Acetic acid (IAA) (0.57, 2.9, 5.7 μM) was evaluated, while maintaining a concentration of BA (13.2 μM). It was observed that the greatest concentration of IAA led to the formation of more than 20 buds per explant. These results offer a new methodology to increase the efficiency of A. tequilana Weber cv. azul-in vitro multiplication by sagittal segmentation of stems and the addition of BA and/or IAA. 相似文献
12.
G.-Y. Kai L.-M. Dai X.-Y. Mei J.-G. Zheng W. Wang Y. Lu Z.-Y. Qian G.-Y. Zhou 《Biologia Plantarum》2008,52(3):557-560
An efficient in vitro plant regeneration system from leaves of Ophiorrhiza japonica Blume was established for the first time. Callus formation rate was more than 90.4 % from leaf segments on Murashige and
Skoog (MS) supplemented with either α-naphthaleneacetic acid (NAA) alone or in combination with 6-benzyladenine (BA). The
highest shoot regeneration (78.9 %) was achieved on MS medium containing 2.0 mg dm−3 BA and 0.2 mg dm−3 NAA, with an average of 9.4 shoots developed per leaf segment. Shoot regeneration was also improved when the leaf explants
were cultured in MS basal medium supplemented with 0.5 % (m/v) polyvinylpyrrolidone (PVP). The leaf explants from seedlings
with age of about 18–27 d showed the highest shoot regeneration. The regenerated shoots were rooted on half-strength basal
MS medium supplemented with 0.5 mg dm−3 indole-3-butyric acid (IBA), which averagely produced 24.8 roots per shoot. The plantlets were transferred to soil, where
100 % survived after 1 month of acclimatization. 相似文献
13.
Tatjana Vujović Đurđina Ružić Radosav Cerović Gordana Šurlan Momirović 《Plant Growth Regulation》2010,61(3):265-275
Adventitious shoot regeneration from leaves of blackberry cultivar Čačanska Bestrna was examined using 30 different combinations
of treatment. Young, fully expanded leaves taken from in vitro proliferating shoots were cultured on Murashige and Skoog (MS)
medium containing either N
6-benzylaminopurine (BAP) (2.0 mg l−1) or thidiazuron (TDZ) (1.0 and 2.0 mg l−1) alone, or either of them combined with indol-3-butyric acid (IBA), α-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic
acid (2,4-D) at different concentrations (0.1, 1.0 and 2.0 mg l−1). Indirect shoot formation was observed in 12 different treatments, though the efficacy varied greatly among types and concentrations
of plant growth regulators. TDZ at 1.0 mg l−1, applied either alone or combined with IBA, was significantly more effective than BAP in inducing shoot regeneration. The
highest regeneration rate (41.66%) was obtained on medium containing TDZ alone. Cytological, flow cytometry and isozyme analyses
were used for screening of genetic stability/instability in regenerants. Cytological study, based on chromosome counts in
root tip meristems, and flow cytometry analysis indicated that adventitious shoots of Čačanska Bestrna are tetraploid with
2n = 4x = 28 as well as those derived from axillary buds. However, a study conducted on the peroxidase patterns of the different
blackberry regenerating lines showed differences between some lines and control plants (in vivo plants and micropropagated
plants). These differences were visible with 3,3′,5,5′-tetramethylbenzidine (TMBZ) as hydrogenous donor for peroxidase detection. 相似文献
14.
The axillary bud-break and multiple bud induction were obtained from the nodal explants of field-grown culms of Bambusa tulda in liquid Murashige and Skoog’s (MS) basal medium supplemented with 2.0 mg l?1 6-benzylaminopurine (BAP), 1.0 mg l?1 kinetin (Kn) and 8% coconut water. Multiple shoots regenerated and proliferated in the liquid MS medium fortified with 3.0 mg l?1 indolebutyric acid (IBA). While, in B. balcooa, MS medium supplemented with 2.5 mg l?1 BAP and 1.0 mg l?1 Kn induced axillary bud-break, bud multiplication and subsequently shoot elongation was obtained after three passages in the same medium. A clump with at least three shoots of both these bamboo species was used as propagule for successful root induction in half-strength MS liquid basal medium supplemented with 0.2 mg l?1 IBA. Sympodial type of microrhizomes developed in B. tulda and the regenerants acclimatized in the soil easily. Explants collected in the month of October produced best in vitro regeneration response in these two bamboo species. Endogenous phenol content proved detrimental for efficient shoot regeneration. The clonal fidelity of the regenerants was established by RAPD analysis advocating clonal propagation through axillary meristem culture of B. balcooa and B. tulda is reliable for commercial exploitation. 相似文献
15.
《Saudi Journal of Biological Sciences》2022,29(5):3425-3431
Present investigation was carried out to arrive at an effective micropropagation protocol for Winter Jasmine (Jasminum nudiflorum) using nodal segments from actively growing plants as explants. Explants were collected from current season shoots during April-May just after the initiation of new flush. Combined sterilization treatment of explants with 1.0% NaOCl2 for 10 min followed by 70% ethanol for 10 s recorded highest culture survival (63.88%) and optimum culture asepsis (63.88%) followed by the treatment containing 0.1% HgCl2 for 10 min followed by 70% ethanol for 10 s with culture survival (61.11%) and culture asepsis (69.44%). Highest culture establishment (80.55%) and minimum days to bud sprouting (7.62 days) was recorded with Benzyl adenine + Kinetin (3.0 + 1.0 mgL?1) but maximum length (4.33 cm) and leaf number (7.78) of established micro shoots was recorded with Benzyl adenine + Kinetin (1.0 + 0.5 mgL?1). Maximum proliferated shoots (2.41) and an optimum proliferation percentage (77.78 %) was recorded with Benzyl adenine + Kinetin (3.0 + 0.5 mgL?1). Minimum size of proliferated shoots (2.02 cm) was recorded with Benzyl adenine + Kinetin (3.0 + 1.0 mgL?1) followed by 2.25 cm recorded with Benzyl adenine + Kinetin (3.0 + 0.5 mgL?1). Highest rooting (63.93%), primary root number/microshoot (4.74) and longest primary roots (34.67 mm) were recorded with IBA (2.0 mgL?1). IBA yielded better results than NAA in terms of higher rooting percentage and root number. However, days to root initiation were found minimum (22.00) with 2.0 mgL?1 of NAA. Highest ex vitro survival of rooted microshoots (89.67%) was recorded with IBA (2.0 mgL?1). 相似文献
16.
N. Irvani M. Solouki M. Omidi A. R. Zare S. Shahnazi 《Plant Cell, Tissue and Organ Culture》2010,100(3):293-299
Dorema ammoniacum D. Don. (Apiaceae), a native medicinal plant in Iran, is classified as a vulnerable species. Root, hypocotyl, and cotyledon
segments were cultured on Murashige and Skoog (MS) (1962) medium supplemented with either 2,4-dichlorophenyoxyacetic acid
(2,4-D) or naphathalene acetic acid (NAA), at 0–2 mg l−1, alone or in combination with either benzyladenine (BA) or kinetin (KN), at 0–2 mg l−1 for callus induction. The best response (100%) was observed from root segments on MS medium containing 1 mg l−1 NAA and 2 mg l−1 BA. The calli derived from various explants were subcultured on MS medium supplemented with BA (1–4 mg l−1) alone or in combination with NAA or indole-3-butyric acid (IBA), at 0.2 or 0.5 mg l−1 for shoot induction. Calli derived from hypocotyl segments showed significantly higher frequency of plantlet regeneration
and number of plantlets than the calli derived from root and cotyledon segments. Therefore, MS medium supplemented with 2 mg l−1 BA and 0.2 mg l−1 IBA produced the highest frequency of shoot regeneration (87.3%) in hypocotyl-derived callus. The optimal medium for rooting
contained 2.5 mg l−1 IBA on which 87.03% of the regenerated shoots developed roots with an average number of 5.2 roots per shoots within 30 days.
These plantlets were hardened and transferred to the soil. The described method can be successfully employed for the large-scale
multiplication and conservation of germplasm this plant. 相似文献
17.
Bilal Haider Abbasi Murad Khan Bin Guo Saleem Ahmed Bokhari Mir Ajab Khan 《Plant Cell, Tissue and Organ Culture》2011,105(3):337-344
The regeneration potential and antioxidative enzyme activities of economically important Brassica rapa var. turnip were evaluated. Calli were induced from leaf explants of seed-derived plantlets on Murashige and Skoog (MS) medium incorporated
with different concentrations of various plant growth regulators (PGRs). The highest leaf explant response (83%) was recorded
for 2.0 mg l−1 benzyladenine (BA) and 1.0 mg l−1 α-naphthaleneacetic acid (NAA). Subsequent subculturing of callus after 3 weeks of culture, on medium with similar compositions
of PGRs, induced shoot organogenesis. The highest shoot induction response (83%) was recorded for 5.0 mg l−1 BA after 5 weeks of transfer. However, 7.8 shoots/explant were recorded for 2.0 mg l−1 BA. The transferring of shoots to elongation medium resulted in 5.1-cm-long shoots on 10 mg l−1 of gibberellic acid (GA3). Rooted plantlets were obtained on MS medium containing different concentrations of indole butyric acid (IBA). The determination
of activities of antioxidative enzymes (superoxide dismutase [SOD], ascorbate peroxidase [APX], catalase [CAT], glutathione
peroxidase [GPX], and peroxidase [POD]) revealed involvement of these enzymes in callus formation and differentiation. All
of the activities were interlinked with each other and played significant roles in the scavenging of toxic free radicals.
This study will help in the advancement of a regeneration protocol for B. rapa var. turnip and the understanding of the functions of antioxidative enzymes in plant differentiation. 相似文献
18.
Maryam Zakavi Hossein Askari Neda Irvani 《Physiology and Molecular Biology of Plants》2016,22(4):583-593
The present study concentrated on introducing a micropropagation protocol for a drought resistant genotype from Pyrus boissieriana, which is the second most naturally widespread pear species in Iran with proper physiological and medicinal properties. Proliferating microshoot cultures were obtained by placing nodal segments on MS medium supplemented with BAP and IBA or NAA. The highest number of shoots (27 shoots per explant) were obtained with 1.5 mg l?1 BAP and 0.05 mg l?1 IBA, but this combination did not produce shoots of desirable length (>1.7 cm). Combination of 1.75 mg l?1 BAP and 0.07 mg l?1 IBA was the best for the shoot multiplication in P. boissieriana with a sufficient number of shoot production (22.33 shoots per explant) and relatively more appropriate shoot length. The larger and greenish leaves were obtained when PG was added to the best multiplication treatment. Microshoot elongation was carried out in 1/2 and 1/4 MS medium containing 50–100 mg l?1 PG with different concentrations of IBA or NAA at intervals of 30–60 days. Significant increase in shoot length was detected after 45–60 days of culture in the presence of PG. The highest shoot length (8 cm) was recorded on 1/2 MS medium supplemented with 0.5 mg l?1 IBA and 100 mg l?1 PG. GA3 negatively affected number and length of shoots and generally caused generation of red leaves. The highest percentage of root induction (100%) and root length (9 cm) were obtained on 1/6 strength MS medium supplemented with 0.005 mg l?1 IBA. All plantlets were hardened when transferred to ex vitro conditions through a period of 25–30 days. The results suggest axillary shoot proliferation of P. boissieriana could successfully be employed for propagation of candidate drought resistant seedling. 相似文献
19.
Sanghamitra Nayak 《Plant Growth Regulation》2000,32(1):41-47
Shoot multiplication and plant regeneration was achieved from freshly sprouted shoots of Curcuma aromatica on Murashige and Skoog's medium supplemented with BA alone (1–7 mgL–1) or a combination of BA(1–5 mgL–1) and Kn (0.5–1 mgL–1). A concentration of 5 mgL–1 BA was optimum for shoot multiplication and rooting of shoots. The regenerated plants grew profusely on transfer to liquid medium.In vitro raised plants were successfully established in the field. Microrhizomes were induced at the base of the in vitro derived shoots upon transfer to medium containingvarious combinations and concentrations of sucrose and BA and grown under varying photoperiods. MS basal medium with 5 mgL–1 BA, 60 gL–1 sucrose and an8 h photoperiod was optimum for induction ofmicrorhizomes within 30 days of culture. Harvestedmicrorhizomes stored in moist sand in poly-bagssprouted after 2 months of storage at roomtemperature. For in vitro storage, microrhizomeswere grown in medium containing 0.1 mgL–1 BA.Microrhizome formation was found to be controlled bythe concentrations of BA and sucrose as well asphotoperiod during culture. 相似文献
20.
Yasmin Vázquez-Santos Yuriana Martínez-Orea Javier Álvarez-Sánchez Noé Manuel Montaño Sara Lucía Camargo-Ricalde Silvia Castillo-Argüero 《Phyton》2021,90(2):605-619
The aim of this study was to analyze the performance of Acaena elongata colonized by arbuscular mycorrhizal
fungi (AMF) to different phosphorus (P) concentrations, as a measure of AMF dependency. A. elongata, is a species from soils where P availability is limited, such as temperate forests. Our research questions were: 1) How do
different P concentrations affect the AMF association in Acaena elongata, and 2) How does the AMF association
influence A. elongata’s growth under different P concentrations? A. elongata’s growth, P content in plant tissue,
AMF colonization and dependency were measured under four P concentrations: control (0 g P kg−1
), low
(0.05 g P kg−1
), intermediate (0.2 g P kg−1
) and high (2 g P kg−1
) in different harvests. A complete randomized
block design was applied. A. elongata’s growth was higher under -AMF in intermediate and high P concentrations, and the lowest growth corresponded to +AMF in the low and intermediate P concentration. We observed
a negative effect on the root biomass under +AMF in intermediate P concentration, while the P concentration had
a positive effect on the leaf area ratio. The AMF colonization in A. elongata decreased in the highest P concentration and it was favored under intermediate P concentration; while the low and the high concentrations generated a cost-benefit imbalance. Our results suggest that the performance of some plant species in soils with
low P availability may not be favored by their association with AMF, but a synergy between AMF and intermediate P concentrations might drive A. elongata’s growth. 相似文献