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Stem cell pluripotency and transcription factor Oct4 总被引:8,自引:1,他引:7
GUANGJINPAN ZENGYICHANG HANSR.SCHOELER DUANQINGPEI 《Cell research》2002,12(5):321-330
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Stem cell pluripotency and transcription factor Oct4 总被引:4,自引:0,他引:4
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Stem cell pluripotency and transcription factor Oct4 总被引:10,自引:1,他引:9
GUANG JIN PAN ZENG YI CHANG HANS R. SCHOLER DUANQING PEI Departments of Pharmacology Biological Sciences & Biotechnology Schools of Sciences Medicine Tsinghua University Beijing China Center for Animal Transgenesis Germ Cell Research School of Veterinary Medicine Department of Animal Biology University of Pennsylvania New Bolton Center West Street Road Kennett Square PA USA Department of Pharmacology University of Minnesota Minneapolis MN USA 《Cell research》2002,(Z2)
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Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells 总被引:14,自引:0,他引:14
Okumura-Nakanishi S Saito M Niwa H Ishikawa F 《The Journal of biological chemistry》2005,280(7):5307-5317
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Selective influence of Sox2 on POU transcription factor binding in embryonic and neural stem cells 下载免费PDF全文
Tapan Kumar Mistri Arun George Devasia Lee Thean Chu Wei Ping Ng Florian Halbritter Douglas Colby Ben Martynoga Simon R Tomlinson Ian Chambers Paul Robson Thorsten Wohland 《EMBO reports》2015,16(9):1177-1191
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Card DA Hebbar PB Li L Trotter KW Komatsu Y Mishina Y Archer TK 《Molecular and cellular biology》2008,28(20):6426-6438
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Sonia Stefanovic Nesrine Abboud Stéphanie Désilets David Nury Chad Cowan Michel Pucéat 《The Journal of cell biology》2009,186(5):665-673
Oct4 exerts a dose-dependent dual action, as both a gatekeeper for stem cell pluripotency and in driving cells toward specific lineages. Here, we identify the molecular mechanism underlying this dual function. BMP2- or transgene-induced Oct4 up-regulation drives human embryonic and induced pluripotent stem cells to become cardiac progenitors. When embryonic stem cell pluripotency is achieved, Oct4 switches from the Sox2 to the Sox17 promoter. This switch allows the cells to turn off the pluripotency Oct4-Sox2 loop and to turn on the Sox17 promoter. This powerful process generates a subset of endoderm-expressing Sox17 and Hex, both regulators of paracrine signals for cardiogenesis (i.e., Wnt, BMP2) released into the medium surrounding colonies of embryonic stem cells. Our data thus reveal a novel molecular Oct4- and Sox17-mediated mechanism that disrupts the stem cell microenvironment favoring pluripotency to provide a novel paracrine endodermal environment in which cell lineage is determined and commits the cells to a cardiogenic fate. 相似文献
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Masamitsu N. Asaka Kousuke Uranishi Ayumu Suzuki Masataka Hirasaki Masazumi Nishimoto Akihiko Okuda 《Development, growth & differentiation》2017,59(8):639-647
The Oct4 gene is a master regulator of the pluripotent properties of embryonic stem cells (ESCs). Recently, Oct4 loci were shown to frequently localize in close proximity to one another during the early stage of cellular differentiation, implicating this event as an important prerequisite step for ESCs to exert their full differentiation potential. Although the differentiation capacity of embryonal carcinoma cells (ECCs), such as F9 and P19 ECC lines, is severely restricted compared with ESCs, ECCs bear a highly similar expression profile to that of ESCs including expression of Oct4 and other pluripotency marker genes. Therefore, we examined whether allelic pairing of Oct4 loci also occurs during differentiation of F9 and P19 ECCs. Our data clearly demonstrate that this event is only observed within ESCs, but not ECCs, subjected to induction of differentiation, indicating transient allelic pairing of Oct4 loci as a specific feature of pluripotent ESCs. Moreover, our data revealed that this pairing did not occur broadly across chromosome 17, which carries the Oct4 gene, but occurred locally between Oct4 loci, suggesting that Oct4 loci somehow exert a driving force for their allelic pairing. 相似文献