共查询到20条相似文献,搜索用时 15 毫秒
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M G Peterson 《Nucleic acids research》1988,16(22):10915
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A Ishihama 《Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme》1985,30(10):1127-1129
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Reverse transcriptase activity in mature spermatozoa of mouse 总被引:1,自引:0,他引:1
Giordano R Magnano AR Zaccagnini G Pittoggi C Moscufo N Lorenzini R Spadafora C 《The Journal of cell biology》2000,148(6):1107-1113
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Inhibition of Taq DNA polymerase by catalpol. 总被引:2,自引:0,他引:2
C R Pungitore M Juri Ayub E J Borkowski C E Tonn G M Ciuffo 《Cellular and molecular biology, including cyto-enzymology》2004,50(6):767-772
DNA polymerases have recently emerged as important cellular targets for chemical intervention in the development of anti-cancer agents. This report describes a PCR assay as a method to investigate the action mechanism of the inhibition of Taq DNA polymerase by catalpol. This inhibition was not primer or template specific, nor was it due to chelation of Mg2+ ions. In assays of hyperchromicity of double-stranded DNA, catalpol did not affect melting profile. The inhibitory effect of catalpol does not appear to depend on DNA concentration. In contrast, increasing dNTP concentration rescue the Taq DNA polymerase activity, suggestingthat catalpol acts in a competitive way with dNTPs at the binding site of the enzyme. Theoretical calculations reinforce the experimental data and the proposed mode of action of catalpol. 相似文献
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F G Pluthero 《Nucleic acids research》1993,21(20):4850-4851
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Polymerases with proofreading activity provide high fidelity PCR amplifications. In this study we examined the consequences of using a Taq polymerase with proofreading activity, such as Optimase Taq polymerase, in combination with 4 different mutagenic reverse primers for the amplification of a 345-bp FII PCR product. The amplifications were performed with Optimase Taq polymerase (Transgenomic), and Taq DNA polymerase-recombinant (Invitrogen), without proofreading activity. Mutation screening was carried out by DHPLC and restriction fragment analysis. The usage of Optimase Taq polymerase results in complete reversion of the first and second mutated nucleotide introduced at the 3' end of the mutagenic reverse primer. It also partially reverses the missense nucleotide introduced in the third position of the mutagenic primer and leads to misleading DHPLC and restriction fragment analysis patterns. Nevertheless it cannot perform such an activity when an abnormal nucleotide is introduced in the fourth position. 相似文献
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Reverse transcriptase in bacteria 总被引:5,自引:4,他引:1
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Reverse transcriptase and neoplasia 总被引:2,自引:0,他引:2
R C Gallo 《Biomedicine / [publiée pour l'A.A.I.C.I.G.]》1973,18(6):446-452
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