Preparation, characterization and properties of superabsorbent nanocomposites based on natural guar gum and modified rectorite

Novel guar gum-g-poly(sodium acrylate)/rectorite (GG-g-PNaA/REC) superabsorbent nanocomposites were prepared in aqueous solution using guar gum (GG), partially neutralized acrylic acid (NaA), acidified rectorite (H⁺-REC) and organified rectorite (CTA⁺-REC) by cetyltrimethylammonium bromide (CTAB) as raw materials, ammonium persulfate (APS) as initiator and N,N′-methylenebisacrylamide (MBA) as crosslinker. FTIR spectra confirmed that NaA had been grafted onto GG chains and the OH groups of REC participated in polymerization reaction. Exfoliated nanocomposite was obtained for H⁺-REC and intercalated structure was formed for CTA⁺-REC as shown by XRD results. SEM observations show REC has been uniformly dispersed in polymeric matrix. Effects of HCl concentration, organification degree of CTA⁺-REC and content of REC on swelling capabilities were investigated and the swelling kinetics of nanocomposites was evaluated. Results indicate that modifying REC by acidification and organification can improve swelling properties of the resultant nanocomposites, and GG-g-PNaA/CTA⁺-REC exhibited higher swelling capability and swelling rate contrast to GG-g-PNaA/H⁺-REC.     

Biodegradable superabsorbent hydrogels derived from cellulose by esterification crosslinking with 1,2,3,4-butanetetracarboxylic dianhydride

Superabsorbent hydrogels were prepared from native celluloses dissolved in lithium chloride and N-methyl-2-pyrrolidinone (LiCl/NMP) by esterification crosslinking with 1,2,3,4-butanetetracarboxylic dianhydride (BTCA). Subsequent conversion of the unreacted carboxyl groups to sodium carboxylates by the addition of aqueous NaOH was performed to enhance the water affinity of the gels. The absorbency of the products was strongly dependent on the amount of BTCA that was esterified to cellulose, and the highest absorbency was observed for the hydrogel composed of approximately 0.25 molecules of BTCA per anhydroglucose unit (AGU) of cellulose. Furthermore, it was confirmed that the absorbency was enhanced as the average degree of polymerization (DP) of the starting cellulose increased. The use of cotton cellulose with a high DP of about 2400 produced a hydrogel with an absorbency of 720 times its dry weight, which exceeded the absorbency of commercial crosslinked sodium polyacrylate superabsorbent hydrogel (SPA). The hydrogels exhibited good biodegradability, with a maximum degradation of 95% within 7 days using cellulase.     

明胶/海藻酸钠/沙蒿胶复合水凝胶的制备及表征

为探究明胶（G）、海藻酸钠（SA）,沙蒿胶（ASKG）对复合水凝胶的力学性能、溶胀和保湿性能的影响,采用共混-离子交联法制备海藻酸钠/明胶/沙蒿胶复合水凝胶,并对制得的水凝胶进行结构表征和溶血率测试。结果表明:当G质量分数为2.5%,SA为1.5%,ASKG为0.7%时,复合水凝胶压缩强度达到427.2 kPa,拉伸强度达到563.449 kPa,断裂伸长率为117%,溶胀率为744%,且具有较好的保湿性能。红外光谱表明,由于沙蒿胶中存在大量羟基,因此加入沙蒿胶后在3 300 cm^-1～3 600 cm^-1羟基峰形变宽。G/SA/ASKG复合水凝胶溶血率低于5%,具有较好的网络孔结构和血液相容性,为复合水凝胶在医用敷料方面的应用提供一定的参考价值。     

Synthesis and characterization of a novel cellulose-g-poly(acrylic acid-co-acrylamide) superabsorbent composite based on flax yarn waste

A new, low-cost, and eco-friendly cellulose-based superabsorbent was successfully prepared from flax yarn waste. The method used was a free-radical graft copolymerization of AA and AM onto a cellulose backbone in a homogeneous aqueous solution. APS was used as the initiator in the presence of a crosslinker, MBA. The effects of various factors on water absorbency were discussed. The factors included reaction temperature, initiator amount, monomer amount, salt solution type, and solution pH. Under the optimized conditions, the water absorbencies of the obtained superabsorbent composite were 875 g/g distilled water, 490 g/g natural rainwater, and 90 g/g 0.9 wt% aqueous NaCl solution. The product also had excellent water retention and salt resistance properties. Fourier-transform infrared spectroscopy and scanning electron microscopy were employed to examine the structure of the prepared superabsorbent.     

Antimicrobial and insecticidal activities of cashew tree gum exudate

The gum exudate of the cashew tree (Anacardium occidentale) was shown to inhibit growth of some fungi and bacteria. The gum also prevented oviposition and reduced the number of surviving adults of the Bruchid Callosobruchus maculatus. The feeding of larvae of the Chrysomelid Crimissa cruralis was also strongly affected by the gum. These results suggest an active role for the gum in the defence mechanisms of the plant.     

Encapsulation of ciprofloxacin within modified xanthan gum- chitosan based hydrogel for drug delivery

The aim of the present work was to investigate the preparation of polyelectrolyte hydrogel as potential drug carrier for antibacterial Ciprofloxacin drug (CFX), intended for controlled release formulation. Hydrogel of N-trimehtyl chitosan (TMC)/sodium carboxymethyl xanthan gum (CMXG) was prepared and ciprofloxacin was employed as a model drug to investigate the loading and release performance of the prepared hydrogel. FTIR, DSC, TGA and SEM analysis were used to characterize the TMC/CMXG hydrogel and its CFX loaded hydrogel. The results showed that the ciprofloxacin was successfully incorporated and released from the prepared hydrogel without the loss of structural integrity or the change in its functionality. The encapsulation efficiency of CFX within the prepared hydrogel was found to be increased with increasing the concentration of drug reaching about 93.8 ± 2.1% with concentration of CFX 250 µg/ml. It was shown also that the drug is entrapped within the gel without significant interaction as confirmed from FTIR spectra and DSC analysis. In vitro release study in phosphate buffer saline (PBS), indicated the steady rise in cumulative drug release with the highest release amount, reaching about 96.1 ± 1.8% up to 150 min, whereby the gel with high drug loading efficiency (3.52 ± 0.07%) displayed faster and higher release rate than that of gel containing a smaller amount of drug (0.44 ± 0.01%). The release kinetics of loaded drug followed zero-order kinetics. CFX drug loaded hydrogel showed high activity against the gram positive and gram negative bacterial strains due to the successful released of CFX from the CFX loaded hydrogel into the tested bacterial strains with the highest diameter of inhibition zone against Escherichia coli (67.0 ± 1.0) as compared to reference antibiotic, Gentamicin (28 ± 0.5). Cytotoxicity of the prepared hydrogel was examined in vitro using lung human normal cell lines and showed the highest cell viability (97 ± 0.5%) at concentration up to 50 µg/ml. Consequently, TMC/CMXG hydrogel can be proposed as new controlled release drug delivery system.     

Modification of sterculia gum with methacrylic acid to prepare a novel drug delivery system

The present paper deals with the modification of the sterculia gum with methacrylic acid (MAAc) to hydrogels for use in drug delivery. The hydrogels were characterized by SEMs, FTIR and swelling studies. The release dynamics of model anti-ulcer drug (ranitidine hydrochloride) from the hydrogels has been studied for the evaluation of the release mechanism. The values of the diffusion exponent 'n' (0.55, 0.54 and 0.59) and gel characteristic constant 'k' (2.109 x 10(-2), 3.698 x 10(-2) and 2.427 x 10(-2)) have been obtained, respectively, in distilled water, pH 2.2 buffer and pH 7.4 buffer. The release of the drug from the hydrogels occurred through non-Fickian diffusion mechanism.     

Synthesis and characterization of novel guar gum hydrogels and their use as Cu sorbents

To prepare novel hydrogels for use in water technologies, guar gum was subjected to acid hydrolysis. The depolymerized guar gum obtained there from and the native guar gum were oxidized to their respective polycarboxylic forms using NO_x as oxidant. All these polymers were crosslinked with N,N-methylenebisacrylamide, and were used as Cu²⁺ sorbents. The candidate hydrogel exhibiting the highest uptake was used further to investigate the effect of external stimuli on sorption. The sorption on hydrogels was fast as the highest sorption was observed after 2 h at 40 °C and 20 ppm of Cu²⁺ ions. The hydrogel prepared from the oxidized guar gum afforded the maximum sorption capacity of 125.893 mg g⁻¹. Langmuir and Freundlich isotherms, and pseudo second order kinetics matches the experimental data. The evidence of sorption was obtained by characterizing Cu²⁺-loaded hydrogels by FTIR spectroscopy.     

Determination of acrylamide and glycidamide in rat plasma by reversed-phase high performance liquid chromatography

Acrylamide is a widely used monomer that produces peripheral neuropathy. It is metabolized to the epoxide, glycidamide, which is also considered to be neurotoxic. A new reversed-phase high-performance liquid chromatography (HPLC) method is described that permits simultaneous determination of acrylamide and glycidamide in rat plasma. Samples were deproteinized with acetonitrile and chromatography was performed using isocratic elution and UV absorption detection. The limits of detection for acrylamide and glycidamide were 0.05 and 0.25 μg/ml in plasma, respectively, and recovery of both analytes was greater than 90%. The assay was linear from 0.1 to 100 μg/ml for acrylamide and from 0.5 to 100 μg/ml for glycidamide. Variation over the range of the standard curve was less than 15%. The method was used to determine the concentration–time profiles of acrylamide and glycidamide in the plasma of acrylamide-treated rats.     

Graft copolymerization of acrylamide on chitosan-co-chitin and its application for immobilization of Aspergillus sp. RL2Ct cutinase

The synthesis of chitosan (Chs) and chitin (Chi) copolymer and grafting of acrylamide (AAm) onto the synthesized copolymer have been carried out by chemical methods. The grafted copolymer was characterized by FTIR, SEM and XRD. The extracellular cutinase of Aspergillus sp. RL2Ct (E.C. 3.1.1.3) was purified to 4.46 fold with 16.1% yield using acetone precipitation and DEAE sepharose ion exchange chromatography. It was immobilized by adsorption on the grafted copolymer. The immobilized enzyme was found to be more stable then the free enzyme and has a good binding efficiency (78.8%) with the grafted copolymer. The kinetic parameters K_M and V_max for free and immobilized cutinase were found to be 0.55 mM and 1410 μmol min⁻¹ mg⁻¹ protein, 2.99 mM and 996 μmol min⁻¹ mg⁻¹ protein, respectively. The immobilized cutinase was recycled 64 times without considerable loss of activity. The matrix (Chs-co-Chi-g-poly(AAm)) prepared and cutinase immobilized on the matrix have potential applications in enzyme immobilization and organic synthesis respectively.     

改性黄原胶酯化条件的优化及结构的表征

为了提高黄原胶的速溶性和粘度,将黄原胶进行改性处理。将黄原胶与马来酸酐进行酯化反应,探讨了黄原胶与马来酸酐摩尔比、反应时间和反应温度等因素的影响,以取代度为指标,利用响应面方法确定,该酯化反应的最优条件为:黄原胶与马来酸酐摩尔比1∶11.5、反应时间24.4 h、反应温度66℃。对改性黄原胶进行红外光谱、光散射和X-射线衍射等结构表征,表明酯化改性成功,且进一步解释了速溶性和粘度提高的原因。改性黄原胶细胞毒性实验,显示无毒性。结果表明,改性黄原胶的速溶性和粘度有很大提高,0.2%改性黄原胶的速溶性和粘度较对照提高了近3倍,在食品、药品等领域具有潜在的应用价值。     

In vivo evaluation of modified gum karaya as a carrier for improving the oral bioavailability of a poorly water-soluble drug,nimodipine

This work examines the influence of modified gum karaya (MGK) on the oral bioavailability of a poorly water-soluble drug, nimodipine (NM), in comparison with that of gum karaya (GK). A cogrinding method was selected to prepare mixtures of NM and GK or MGK in a 1:9 ratio (NM:GK/MGK). Differential scanning calorimetry (DSC), Fourier transmission infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), solubility studies, and in vitro release studies were performed to characterize the properties of the cogrinding mixtures. No drug-carrier interactions were found, as confirmed by DSC and FT-IR studies. The XRD study revealed that the crystallinity of NM was identical in both the cogrinding mixtures and was decreased when compared to that of physical mixtures or pure NM. The in vitro release rate of NM from both cogrinding mixtures was significantly higher than that of physical mixtures or pure NM. The in vivo study revealed that the bioavailability of NM from pure drug was significantly lower when compared to the cogrinding mixtures. The oral bioavailability was found to be NM powder < cogrinding mixtures of NM and GK < cogrinding mixtures of NM and MGK < NM solution. It can be inferred from the above results that MGK, an economical carrier, could be used for the dissolution enhancement of NM.     

Polymer hydrogel from carboxymethyl guar gum and carbon nanotube for sustained trans-dermal release of diclofenac sodium

Novel carboxymethyl guar gum (CMG)-chemically modified multiwalled carbon nanotube (MCNT) hybrid hydrogels were synthesized at different MCNT levels as potential device for sustained trans-dermal release of diclofenac sodium. Spectroscopy together with morphology, thermogravimetry, and rheological studies proved relatively strong CMG-MCNT interaction at 0.5 and 1 wt% levels of MCNT whereas de-wetting was increased with higher MCNT concentration. Drug encapsulation tendency increased with addition of MCNT; maximum entrapment was noticed at 1 wt% MCNT level. Hydrogels containing 0.5, 1 and 3 wt% MCNT exhibited slower trans-dermal release than neat CMG due to slightly higher gel viscosity and more drug entrapment. Slowest but steady release was obtained from 1 wt% MCNT loaded hydrogel due to highest viscous resistance among all other hybrid nanocomposites.     

Improvement of decay resistance of wood via combination treatment on wood cell wall: Swell-bonding with maleic anhydride and graft copolymerization with glycidyl methacrylate and methyl methacrylate

Poplar wood (Populus ussuriensis Kom) was modified by a novel combined two-step treatment to improve its decay resistance. Maleic Anhydride (MAN) was first employed to swell and bond to wood cell wall, and then mixed monomers of glycidyl methacrylate/methyl methacrylate (GMA/MMA) were used to graft copolymerization within wood cell lumen. The swelling and bonding of cell wall by MAN, interfacial compatibility between resultant polymer from GMA/MMA monomers and wood cell wall, and decay resistance of all composites were tested and analyzed by Scanning electron microscopy–Energy dispersive X-ray (SEM–EDX), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) apparatus. The results indicate that the volume of poplar wood treated by MAN swells about 9% with about 15% of weight percent gain, and MAN chemically bonds to the cell wall through substitution reaction with hydroxyl group, and the grafting adduct mainly remains as an amorphous form. The resultant Poplar-MAN shows improved decay resistance of 69.79% against brown fungus (Gloeophyllum trabeum (Pers. ex Fr.) Murr.) and 81.42% against white fungus (Phanerochaete chrysosporium Burdsall.) over those of untreated Poplar, respectively. After the combined two-step treatment, GMA and MMA are copolymerized within wood cell lumen, and the resultant polymer is also grafted onto wood cell wall, resulting in the improvement of interfacial compatibility between polymer and wood substance without obvious gaps. The decay resistance of the resultant composite from the combined two-step treatment against the brown decay fungus and the white decay fungus is improved by 97.64% and 99.17%, respectively, compared with those of untreated poplar wood; and also more excellent than those of MMA treated wood, GMA/MMA monomers treated wood, organic 3-Iodo-2-Propynyl Butyl Carbamate (IPBC) treated wood and inorganic boron compounds treated wood, respectively.     

Cancer chemopreventive and anti-inflammatory activities of chemically modified guar gum

Guar gum (G) is a simple characterized branched polysaccharide, which is frequently used in food industries. We prepared the gum C-glycosylated derivative (GG), and its sulphated derivative (SGG), aiming to characterize their cancer chemopreventive, and anti-inflammatory properties. Estimation of cancer chemopreventive activity, specifically anti-initiation, including the modulation of carcinogen metabolism and the antioxidant capacity, revealed that GG was a potent anti-initiator, where it inhibited not only the carcinogen activator enzyme, cytochrome P450 1A (CYP1A), but also induced the carcinogen detoxification enzymes glutathione-S-transferases (GSTs), while SGG inhibited both CYP1A and GSTs. SGG was an effective radical scavenger than GG against hydroxyl, peroxyl, and superoxide anion radicals. GG and SGG were found to modulate the macrophage functions into an anti-inflammatory pattern. Thus, both enhanced the macrophage proliferation and phagocytosis of fluorescein isothiocyanate (FITC)-zymosan; however, they also inhibited strongly the nitric oxide generation and tumor necrosis factor-alpha secretion in lipopolysaccharide (LPS)-stimulated RAW macrophage 264.7. Unexpectedly, both GG and SGG dramatically inhibited the binding affinity of FITC-LPS to RAW 264.7, as indicated by flow cytometry analysis. GG and SGG exhibited a significant anti-proliferative activity against human hepatocellular carcinoma cells (Hep G2), and only SGG was specifically cytotoxic for human breast carcinoma cells (MCF-7), but neither was significantly cytotoxic for human lymphoblastic leukemia cells (1301). SGG led to a major disturbance in cell cycle phases of Hep G2 cells as indicated by concomitant arrest in S- and G2/M-phases, a disturbance that was associated with an induced cell death as a result of necrosis, but not apoptosis in both GG- and SGG-treated cells. Taken together, the modified gums could be used as an alternative of G in health food industries to provide cancer prevention in risk populations.     

Synthesis of oxidized guar gum by dry method and its application in reactive dye printing

The aim of this study was to prepare oxidized guar gum with a simple dry method, basing on guar gum, hydrogen peroxide and a small amount of solvent. To obtain a product with suitable viscosity for reactive dye printing, the effects of various factors such as the amount of oxidant and solvent, reaction temperature and time were studied with respect to the viscosity of reaction products. The product was characterized by Fourier transform infrared spectroscopy, size exclusion chromatography, scanning electron microscopy and differential scanning calorimetry. The hydrated rate of guar gum and oxidized guar gum was estimated through measuring the required time when their solutions (1%, w/v) reached the maximum viscosity. The effects of the salt concentration and pH on viscosity of the resultant product were studied. The mixed paste containing oxidized guar gum and carboxymethyl starch was prepared and its viscosity was determined by the viscometer. The rheological property of the mixed paste was appraised by the printing viscosity index. In addition, the applied effect of mixed paste in reactive dye printing was examined by assessing the fabric stiffness, color yield and sharp edge to the printed image in comparison with sodium alginate. And the results indicated that the mixed paste could partially replace sodium alginate as thickener in reactive dye printing. The study also showed that the method was low cost and eco-friendly and the product would have an extensive application in reactive dye printing.     

Graft copolymerization of acrylic acid onto guar gum initiated by vanadium (V)–mercaptosuccinic acid redox pair

Guar gum has been modified by graft copolymerization with acrylic acid in aqueous medium using vanadium (V)–mercaptosuccinic acid redox system. The optimum reaction conditions affording maximum grafting ratio, efficiency, add on and conversion have been determined. The grafting parameters have been found to increase with increase in vanadium (V) concentration upto 1.0 × 10⁻² mol dm⁻³, but these parameters decrease on further increasing the vanadium (V) concentration. On increasing the mercaptosuccinic acid concentration from 1.0 × 10⁻² to 4.0 × 10⁻² mol dm⁻³ grafting ratio, efficiency and add on increase up to 2.0 × 10⁻² mol dm⁻³ but decrease with further increase in mercaptosuccinic acid concentration. On varying the acrylic acid concentration from 5.0 × 10⁻² to 30.0 × 10⁻² mol dm⁻³, maximum grafting ratio, efficiency and add on have been obtained at 20.0 × 10⁻² mol dm⁻³. The grafting ratio, add on and conversion increase, on increasing the H⁺ ion concentration from 1.5 × 10⁻¹ to 6.0 × 10⁻¹ mol dm⁻³. On increasing the guar gum concentration the grafting parameters increase. The grafting ratio, add on and conversion have been found to increase with time period while efficiency started decreasing after 120 min. It has been observed that %G increases on increasing the temperature up to 35 °C. The graft copolymer has been characterized by IR spectroscopy and thermogravimetric analysis.     

Synthesis and characterization of guar gum templated hybrid nano silica

The objective of the present study was the fabrication of green adsorbent hybrids for which native guar gum was used as template to polymerize tetraethoxysilane. The properties and performances of the hybrids could be tailored by using varying molecular sizes of the partially depolymerized guar gum templates of various molecular sizes as control. Zn(II) uptake from aqueous solution was used as a criterion for evaluating the adsorbent efficiency. The optimum material (H4) in terms of maximum Zn(II) uptake, was obtained when the template size used was 375 kDa at a calcination temperature of 700 °C. H4 was also evaluated for Ca(II), Mg(II), Cd(II) and Hg(II) adsorption. To explore the other applicability areas, the hybrids have been extensively characterized using FTIR, XRD, TGA-DTA, PL, SEM, TEM and BET analyses. H4 was found to be as efficient as previously reported vinyl modified-silica nanohybrids. It had a high surface area (264 m²/g) with silica nanoparticles in the size range of 90-140 nm. Being thermally very stable and photoluminescent, the material can be potentially used for many biological, medical and environmental applications.     

Production of recombinant plant gum with tobacco cell culture in bioreactor and gum characterization

Many plant gums, such as gum arabic, contain hydroxyproline-rich glycoproteins (HRGPs), which are also abundant components of the plant cell extracellular matrix. Here we expressed in transgenic BY2 Nicotiana tabacum (tobacco) cells, a synthetic gene encoding a novel HRGP-based gum, designated gum arabic-8 or (GA)(8). (GA)(8) encoded eight repeats of the consensus polypeptide sequence of gum arabic glycoprotein (GAGP): Gly-Pro-His-Ser-Pro-Pro-Pro-Pro-Leu-Ser-Pro-Ser-Pro-Thr-Pro-Thr-Pro-Pro-Leu, in which most of the Pro residues were posttranslationally modified to hydroxyproline (Hyp). (GA)(8) was expressed as a green fluorescent protein (GFP) fusion protein targeted to the culture medium, (GA)(8)GFP. The culture of the transgenic cells in a 5-L bioreactor showed that the production of (GA)(8)GFP was cell growth-associated. The extracellular yield of (GA)(8)GFP was 116.8 mg/L after 14 days of culture and accounted for 87% of the total fusion protein expressed. (GA)(8)GFP was purified from the culture medium by a combination of hydrophobic interaction, gel permeation, and reversed phase chromatography. Biochemical characterization indicated that the amino acid composition of the (GA)(8) module, after removal of GFP by proteolysis, was virtually identical to that of predicted by the GAGP consensus sequence and that carbohydrate, which occurred as arabinogalactan polysaccharides and small oligoarabinosides O-linked through the Hyp residues, accounted for 84% of the molecules' dry weight. Functional assays showed that (GA)(8) exhibited low viscosity in aqueous solution similar to native GAGP. However, neither GFP alone nor the (GA)(8) module could emulsify orange oil. However, the fusion protein (GA)(8)GFP possessed 1.28-fold better emulsification properties than native GAGP. This work demonstrates the feasibility and potential of a synthetic gene approach to the de novo design of novel glycoprotein-based gums and emulsifiers.     

The swelling behavior and network parameters of guar gum/poly(acrylic acid) semi-interpenetrating polymer network hydrogels

Guar gum/poly(acrylic acid) semi-interpenetrating polymer network (IPN) hydrogels have been prepared via free radical polymerization in the presence of a crosslinker of N,N′-methylene bisacrylamide (MBA). The kinetics of swelling and the water transport mechanism were studied as a function of the composition of the hydrogels and the pH of the swelling medium. Hydrogels showed enormous swelling in aqueous medium and displayed swelling characteristics, which were highly dependent on the chemical composition of the hydrogels and pH of the medium in which hydrogels were immersed (ionic strength I = 0.15 mol/L). The semi-INP hydrogels were characterized by evaluating various network parameters such as average molecular weight between crosslinks (M_c) crosslink density (ρ) and mesh size ξ.