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1.
Ribosomal Ribonucleic Acid Synthesis and Maturation in the Blue-Green Alga Anacystis nidulans 总被引:7,自引:4,他引:3
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W. Ford Doolittle 《Journal of bacteriology》1972,111(2):316-324
Methods are described for preparation of pulse-labeled ribonucleic acid (RNA) from the blue-green alga Anacystis nidulans. Synthesis of labeled RNA was found to be in part dependent on concurrent photosynthesis and was inhibited by the antibiotic streptolydigin. Mature 23S ribosomal RNA (rRNA) appeared before mature 16S rRNA. Formation of either molecule was inhibited by chloramphenicol, and RNA species of lesser mobility accumulated. These species may be precursors of the mature forms. Maturation of 16S rRNA was also inhibited by streptolydigin. (The effect of this antibiotic on 23S rRNA maturation was not examined). In many respects, ribosomal RNA synthesis and maturation in this blue-green alga appear to follow the pattern already established for bacteria. 相似文献
2.
The alginate forms the major structural component of the cell wall and the intercellular matrix of the brown alga Ascophyllum
nodosum. Successful biological degradation of A. nodosum would largely depend on the dissolution of the alginate, but reactive
compounds in the alga such as polyphenols may also have toxic effects on the microbial population involved. Aerobic and anaerobic
batch reactors, operated at 35°C and pH 7, were fed milled A. nodosum, nutrients and inocula adapted to seaweed degradation.
The dominant factor for conversion of organic matter during anaerobic digestion was the inhibitory effect of the polyphenols
on alginate lyases and methane production. Probably, the relative large fraction of high molecular weight polyphenols (>10
kDa) in this alga gave efficient binding of proteins during digestion. The anaerobic degradation was greatly stimulated when
the polyphenols were fixed with low amounts of formaldehyde. An accumulated content of guluronate in the remaining alginate
indicated that Ca-crosslinking also limited the guluronate lyase access to the polymer. In contrast, the aerobic digestion
of alga gave no increase in the guluronate content of the residual alginate. Compared to anaerobic conditions, the phenols
had a much lower influence on the hydrolytic rate of organic matter during aerobic conditions.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
3.
Structure, Growth, and Decomposition of Laminated Algal-Bacterial Mats in Alkaline Hot Springs 总被引:3,自引:14,他引:3
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Laminated mats of unique character in siliceous alkaline hot springs of Yellowstone Park are formed predominantly by two organisms, a unicellular blue-green alga, Synechococcus lividus, and a filamentous, gliding, photosynthetic bacterium, Chloroflexus aurantiacus. The mats can be divided approximately into two major zones: an upper, aerobic zone in which sufficient light penetrates for net photosynthesis, and a lower, anaerobic zone, where photosynthesis does not occur and decomposition is the dominant process. Growth of the mat was followed by marking the mat surface with silicon carbide particles. The motile Chloroflexus migrates vertically at night, due to positive aerotaxis, responding to reduced O2 levels induced by dark respiration. The growth rates of mats were estimated at about 50 μm/day. Observations of a single mat at Octopus Spring showed that despite the rapid growth rate, the thickness of the mat remained essentially constant, and silicon carbide layers placed on the surface gradually moved to the bottom of the mat, showing that decomposition was taking place. There was a rapid initial rate of decomposition, with an apparent half-time of about 1 month, followed by a slower period of decomposition with a half-time of about 12 months. Within a year, complete decomposition of a mat of about 2-cm thickness can occur. Also, the region in which decomposition occurs is strictly anaerobic, showing that complete decomposition of organic matter from these organisms can occur in the absence of O2. 相似文献
4.
A manometric method for measuring photosynthesis in marine algae is described. Photosynthesis in the red alga Gigartina harveyana is shown to be similar in all important respects to photosynthesis in Chlorella and other Chlorophyceae. 相似文献
5.
The unicellular green alga Chlamydomonas reinhardtii has a special type of anaerobic metabolism that is quite unusual for eukaryotes. It has two oxygen-sensitive [Fe-Fe] hydrogenases (EC 1.12.7.2) that are coupled to photosynthesis and, in addition, a formate- and ethanol-producing fermentative metabolism, which was proposed to be initiated by pyruvate formate-lyase (Pfl; EC 2.3.1.54). Pfl enzymes are commonly found in prokaryotes but only rarely in eukaryotes. Both the hydrogen- and the formate/ethanol-producing pathways are involved in a sustained anaerobic metabolism of the alga, which can be induced by sulfur depletion in illuminated cultures. Before now, the presence of a Pfl protein in C. reinhardtii was predicted from formate secretion and the homology of the deduced protein of the PFL1 gene model to known Pfl enzymes. In this study, we proved the formate-producing activity of the putative Pfl1 enzyme by heterologous expression of the C. reinhardtii PFL1 cDNA in Escherichia coli and subsequent in vitro activity tests of the purified protein. Furthermore, a Pfl-deficient E. coli strain secretes formate when expressing the PFL1 cDNA of C. reinhardtii. We also examined the Pfl1 fermentation pathway of C. reinhardtii under the physiological condition of sulfur depletion. Genetic and biochemical analyses show that sulfur-depleted algae express genes encoding enzymes acting downstream of Pfl1 and also potentially ethanol-producing enzymes, such as pyruvate decarboxylase (EC 4.1.1.1) or pyruvate ferredoxin oxidoreductase (EC 1.2.7.1). The latter enzymes might substitute for Pfl1 activity when Pfl1 is specifically inhibited by hypophosphite. 相似文献
6.
Photolability of Photosynthesis in Two Separate Mutants of Scenedesmus obliquus: Preferential Inactivation of Photosystem I
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Two separate mutants of the green alga, Scenedesmus obliquus, are described in which photosynthesis is sensitive to moderate intensities of white light (100 mw cm−2). Heterotrophic cultures of both mutants lose photosynthetic activity when exposed to white light; the site of at least the initial phase of this inactivation is within photosystem I. Although all whole cell and cell-free reactions typical of photosystem I examined are inhibited by irradiation, the principal component of photosystem I affected is P-700. In light-sensitive-4 the inactivation of P-700 activity is restored during the subsequent dark period. This recovery is prevented by various antibiotics and by anaerobic conditions. In light-sensitive-41 P-700 activity is recovered only after a complete cell division and new growth. Irradiation periods which inhibit photosynthesis in both mutants are without effects upon the activity or presence of ferredoxin, ferredoxin-NADP+ oxidoreductase, plastocyanin, cytochrome f(552), cytochrome b-562 or cytochrome b-559.
Prolonged irradiation of cells of light-sensitive-41 causes the disappearance of photosystem II activity, α-tocopherol, and plastoquinone. Some decrease of both the chlorophylls and carotenoids occurs but there is no preferential deletion of any particular carotenoid.
相似文献7.
Damien G. Lemoine Florian Mermillod-Blondin Marie-Hélène Barrat-Segretain Corinne Massé Emmanuel Malet 《Aquatic Ecology》2012,46(2):191-200
Radial oxygen loss (ROL) has been suggested to be a major process to protect plants exposed to root anaerobic stress. In the present study, we aimed to test the importance of root porosity and radial oxygen loss on the aquatic macrophyte resistance to sediment anoxia. We expected that species living in eutrophic environments characterized by anaerobic conditions in sediments exhibited higher root porosity and radial oxygen loss than species restrained to oligotrophic environments. In this way, we compared the responses to sediment anoxia of two hydrophyte species growing under meso-eutrophic conditions in the field (Myriophyllum spicatum L. and Vallisneria spiralis L.) and three species growing under oligotrophic conditions (Potamogeton coloratus Horne, Elodea canadensis Michx and Sparganium emersum Michx.). Under laboratory conditions, ROL, root porosity, plant metabolism (aerobic respiration, photosynthesis, root fermentative activity) and plant growth were analysed after 3?months of acclimation in anaerobic sediments and compared with control values obtained from aerobic sediments. The results showed that two meso-eutrophic species (M. spicatum and V. spiralis) survived in anaerobic sediments and maintained similar photosynthesis rates than those measured under aerobic conditions. In contrast, the three oligotrophic species (P. coloratus, E. canadensis and S. emersum) suffered net biomass loss and depressed their photosynthesis rates under anaerobic conditions. All variables associated with plant tolerance to anaerobic conditions (maintenance of photosynthesis, aerobic respiration and growth rate, and limitation of root fermentative activity) were positively linked to root porosity and ROL. According to our hypothesis, species that could survive to anaerobic conditions were the species able to increase their root porosity and ROL under these conditions. Thus, in ecological studies, it would be useful to use the root porosity and ROL plasticity as biological traits in order to model the distribution of macrophytes in river floodplains. 相似文献
8.
The effects of ultraviolet irradiation on a coccoid blue-green alga: survival, photosynthesis, and photoreactivation 总被引:11,自引:3,他引:8
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Van Baalen C 《Plant physiology》1968,43(10):1689-1695
The effects of UV irradiation (254 mμ) on a coccoid blue-green alga Agmenellum quadruplicatum, Strain PR-6, have been examined in terms of the survival curve and measurement of short time photosynthetic rates. From study of survival evidence has been found for a strong photoreactivation centered near 430 mμ. Measurements of photosynthetic rate suggest that there is a correlation between decay of photosynthesis and survival after UV exposure. The UV induced decay in photosynthetic activity is reversed by the identical photoreactivation conditions that increase the survival level. The photosynthetic data are interpreted as demonstrating a photoreactivation of photosynthesis in blue-green algae. 相似文献
9.
The green alga Selenastrum minutum (Naeg.) Collins is able to assimilate NH4+ in the dark under anaerobic conditions (GC Vanlerberghe, AK Horsey, HG Weger, DH Turpin [1989] Plant Physiol 91: 1551-1557). In the present study, analysis of metabolites following addition of NH4+ to cells acclimated to anaerobic conditions has shown the following. There was a transient decline in adenylate energy charge from 0.6 to 0.4 followed by a recovery back to ~0.6. This was accompanied by a rapid increase in pyruvate/phosphoenolpyruvate and fructose-1,6-bisphosphate/fructose-6-phosphate ratios indicating activation of pyruvate kinase and 6-phosphofructokinase, respectively. There was also an increase in fructose-2,6-bisphosphate, which, since this alga lacks pyrophosphate dependent 6-phosphofructokinase can be inferred to inhibit gluconeogenic fructose-1,6-bisphosphatase. These changes resulted in an increase in the rate of anaerobic starch breakdown. Anaerobic NH4+ assimilation also resulted in a two-fold increase in the rate of production of the major fermentative end-products in this alga, d-lactate and ethanol. There was no change in the rate of accumulation of the fermentative end product succinate but malate accumulated under anoxia during NH4+ assimilation. A rapid increase in Gln and decline in Glu indicates that primary NH4+ assimilation under anoxia was via glutamine synthetase-glutamate synthase. Almost all N assimilated under these conditions was sequestered in alanine. These results allow us to propose a model for the regulation of carbon metabolism during anaerobic NH4+ assimilation. 相似文献
10.
Characterization of a Photosynthetic Mutant Strain of Chlamydomonas reinhardi Deficient in Phosphoribulokinase Activity 总被引:11,自引:8,他引:3
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A mutant strain of the unicellular green alga, Chlamydomonas reinhardi, is unable to fix carbon dioxide by photosynthesis because it is deficient in phosphoribulokinase activity. The absence of light-dependent carbon dioxide fixation in cells of the mutant strain supports the operation of the Calvin-Benson scheme of photosynthetic carbon dioxide fixation in this organism. No deficiency other than low phosphoribulokinase activity was found which would account for the inability of cells of the mutant strain to fix carbon dioxide by photosynthesis. Activities comparable to those in the wild-type strain were found for eight other enzymes of the Calvin cycle and two enzymes associated with the C4 dicarboxylic acid pathway. The normal rates of nicotinamide adenine dinucleotide phosphate photoreduction and of photosynthetic phosphorylation observed in chloroplast fragments prepared from cells of the mutant strain indicated that the photosynthetic electron transport chain in the mutant is intact. 相似文献
11.
V. B. Borodin 《Russian Journal of Plant Physiology》2013,60(3):420-430
The effect of storage of the unicellular green alga Chlamydomonas reinhardtii (strain 137+) in the pelleted state in darkness on ice (0.2–0.5°C) (further simply “SPDI-treatment”) on its photosynthetic and respiratory activities was studied. To this end, the steady-state rates of O2 exchange in darkness (dark respiration) and under saturating light (apparent photosynthesis) as well as the induction periods (IP) of apparent photosynthesis were measured at 25°C in the SPDI-untreated and SPDI-treated for the period from ~0.5 to ~30 h algal cells. In contrast to expectations, the SPDI-treatment consistently affected the rate and IP of photosynthesis depending on the physiological state of C. reinhardtii. Dark respiration was affected by the SPDI-treatment as well. However, in absolute values the respiratory changes were much less than the photosynthetic ones, and they were insufficiently reproducible. The SPDI-treatment affected photosynthesis most significantly in high-CO2-grown cells (cells grown at 5% CO2 in white light). The rate of photosynthesis in these cells declined quasi-exponentially as a function of time during the SPDI-treatment with a t 1/2 ~1.5 h and finally became by about 60% lower than that before the SPDI-treatment. This decline of photosynthesis was accompanied by continuous and essential increase in the photosynthetic IP. The SPDI-induced photosynthetic changes in high-CO2-grown cells resulted from the firm disfunction of the photosynthetic apparatus. After switch from growth at 5% CO2 in white light to growth at ~0.03% CO2 (air) in white, blue, or red light, the alga gradually transited to a physiological state, in which the negative effects of the SPDI-treatment on the rate and IP of photosynthesis became weak and absent, respectively. Remarkably, this transition was faster in blue (≤5 h) than in white and red light (>10 h). Similar changes in the response of the alga to the SPDI-treatment occurred when high-CO2-grown cells (5% CO2, white light, 26°C) were incubated in darkness (air, 24–26°C) for 20–25 h. The results of study were analyzed in the light of literature data relating to the effects of CO2 concentration, darkness, and light quality on carbohydrates in plant organisms. The analysis led to suggestion that there is connection between the negative effect of the SPDI-treatment on C. reinhardtii and nonstructural carbohydrates presented in the alga: the more carbohydrates contain the alga, the more extensive inactivation of the photosynthetic apparatus occurs in it during its storage in the dense (pelleted) state in darkness on ice. 相似文献
12.
Oxygenic photosynthesis uses light as energy source to generate an oxidant powerful enough to oxidize water into oxygen, electrons and protons. Upon linear electron transport, electrons extracted from water are used to reduce NADP(+) to NADPH. The oxygen molecule has been integrated into the cellular metabolism, both as the most efficient electron acceptor during respiratory electron transport and as oxidant and/or "substrate" in a number of biosynthetic pathways. Though photosynthesis of higher plants, algae and cyanobacteria produces oxygen, there are conditions under which this type of photosynthesis operates under hypoxic or anaerobic conditions. In the unicellular green alga Chlamydomonas reinhardtii, this condition is induced by sulfur deficiency, and it results in the production of molecular hydrogen. Research on this biotechnologically relevant phenomenon has contributed largely to new insights into additional pathways of photosynthetic electron transport, which extend the former concept of linear electron flow by far. This review summarizes the recent knowledge about various electron sources and sinks of oxygenic photosynthesis besides water and NADP(+) in the context of their contribution to hydrogen photoproduction by C. reinhardtii. This article is part of a Special Issue entitled: Regulation of Electron Transport in Chloroplasts. 相似文献
13.
Jack Myers With the Technical Assistance of Marian Cramer James Johnston 《The Journal of general physiology》1947,30(3):217-227
1. An oxidative assimilation of acetic add and glucose in darkness has been demonstrated in the green alga, Chlorella pyrenoidosa. From manometric experiments it has been shown that 1 mol (CH2O) per mol acetic add and 5 mols (CH2O) per mol glucose are produced. 2. The time required for complete utilization of a limited amount of acetic acid or glucose is not affected by illumination in the absence of carbon dioxide. 3. The time required for complete utilization of a limited amount of glucose is not affected by the simultaneous occurrence of photosynthesis. It must therefore be concluded that the accumulating product of photosynthesis cannot be glucose but must be some slowly respirable (storage) material. 4. Possible interrelationships between oxidative assimilation and photosynthesis may be further studied by following, in darkness and in light, the time course of oxidative assimilation of substrates which are possible intermediates in the two processes. 相似文献
14.
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16.
Photosystem I-Mediated Regulation of Water Splitting in the Red Alga, Porphyra sanjuanensis
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The marine red alga, Porphyra sanjuanensis is found mainly in the high intertidal zone and at low tide subject to frequent and extreme water stress, often accompanied by high temperatures and light intensities. Such exposures can lead to severe desiccation which is accompanied by the progressive loss of photosynthetic activity. Even following the loss of more than 90% of the thallus water content the alga recovers rapidly when returned to seawater. This stress-induced, reversible inactivation of photosynthesis is believed to be a protective adaptation which prevents photodamage to the exposed alga. Effects of light, inhibitors of water splitting, and electron donors to PSI on variable fluorescence and water splitting suggest that activity of the oxygen evolving complex is regulated by the PSI-driven reduction of a component of intersystem electron transport. 相似文献
17.
The light-harvesting complex (LHC) is an essential component in light energy capture and transduction to facilitate downstream photosynthetic reactions in plant and algal chloroplasts. The unicellular dinoflagellate alga Symbiodinium is an endosymbiont of cnidarian animals, including corals and sea anemones, and provides carbohydrates generated through photosynthesis to host animals. Although Symbiodinium possesses a unique LHC gene family, called chlorophyll a-chlorophyll c2-peridinin protein complex (acpPC), its genome-level diversity and evolutionary trajectories have not been investigated. Here, we describe a phylogenetic analysis revealing that many of the LHCs are encoded by highly duplicated genes with multi-subunit polyprotein structures in the nuclear genome of Symbiodinium minutum. This analysis provides an extended list of the LHC gene family in a single organism, including 80 loci encoding polyproteins composed of 145 LHC subunits recovered in the phylogenetic tree. In S. minutum, 5 phylogenetic groups of the Lhcf-type gene family, which is exclusively conserved in algae harboring secondary plastids of red algal origin, were identified. Moreover, 5 groups of the Lhcr-type gene family, of which members are known to be associated with PSI in red algal plastids and secondary plastids of red algal origin, were identified. Notably, members classified within a phylogenetic group of the Lhcf-type (group F1) are highly duplicated, which may explain the presence of an unusually large number of LHC genes in this species. Some gene units were homologous to other units within single loci of the polyprotein genes, whereas intergenic homologies between separate loci were conspicuous in other cases, implying that gene unit ‘shuffling’ by gene conversion and/or genome rearrangement might have been a driving force for diversification. These results suggest that vigorous intra- and intergenic gene duplication events have resulted in the genomic framework of photosynthesis in coral symbiont dinoflagellate algae. 相似文献
18.
Stromal acidification mediates in vivo water stress inhibition of nonstomatal-controlled photosynthesis 总被引:5,自引:4,他引:1
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Stromal acidification has been reported to mediate reduced osmotic potential (ψπ) effects on photosynthesis in the isolated spinach chloroplast (Berkowitz, Gibbs 1983 Plant Physiol 72: 1100-1109). To determine if stromal acidification mediates osmotic dehydration inhibition of photosynthesis in vivo, the effects of a weak base (NH4Cl), which raises stromal pH, on CO2 fixation of vacuum-infiltrated spinach leaf slices, Chlamydomonas reinhardii cells and Aphanocapsa 6308 cells under isotonic and dehydrating conditions were investigated. Five millimolar NH4Cl stimulated spinach leaf slice CO2 fixation by 43% under stress (0.67 molar sorbitol) conditions, and had little effect on fixation under isotonic (0.33 molar sorbitol) conditions. Chlamydomonas cells were found to be more sensitive to reduced ψπ than spinach leaf slices. CO2 fixation in the cells of the green alga Chlamydomonas reinhardii was 99 and 17 micromoles per milligram chlorophyll per hour, respectively, at 0.1 molar mannitol and 0.28 molar mannitol. Five millimolar NH4Cl stimulated CO2 fixation of Chlamydomonas cells by 147% under stress (0.28 molar mannitol) conditions. Aphanocapsa 6308 cells (blue-green alga) were also found to be sensitive to reduced ψπ, and inhibitions in photosynthesis were partially reversed by NH4Cl. These data indicate that in vivo water stress inhibition of photosynthesis is facilitated by stromal acidification, and that this inhibition can be at least partially reversed in situ. 相似文献
19.
Mycobacterial Stationary Phase Induced by Low Oxygen Tension: Cell Wall Thickening and Localization of the 16-Kilodalton α-Crystallin Homolog
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Most cases of tuberculosis are due to reactivation of endogenous infection which may have lain quiescent or dormant for decades. How Mycobacterium tuberculosis survives for this length of time is unknown, but it is hypothesized that reduced oxygen tension may trigger the tubercle bacillus to enter a state of dormancy. Mycobacterium bovis BCG and M. tuberculosis H37Rv were cultured under aerobic, microaerobic, and anaerobic conditions. Their ultrastructural morphology was analyzed by transmission electron microscopy (TEM), and protein expression profiles were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). TEM revealed that the microaerobically and anaerobically cultured bacilli but not the aerobically cultured bacilli developed a strikingly thickened cell wall outer layer. The thickening was not observed in aerobically cultured stationary-phase bacilli or in anaerobically cultured Mycobacterium smegmatis. A highly expressed protein was detected by SDS-PAGE in microaerobic and anaerobic cultures and was identified as the 16-kDa small heat shock protein or α-crystallin homolog. Immunolocalization by colloidal gold immunoelectron microscopy identified three patterns of protein distribution in M. bovis BCG cultured under low oxygen tension. The 16-kDa protein was strongly associated with the cell envelope, fibrous peptidoglycan-like structures, and intracellular and peripheral clusters. These results suggest that tubercle bacilli may adapt to low-oxygen conditions by developing a thickened cell wall and that the 16-kDa protein may play a role in stabilizing cell structures during long-term survival, thus helping the bacilli survive the low oxygen tension in granulomas. As such, the cell wall thickening and the 16-kDa protein may be markers for the dormant state of M. tuberculosis. 相似文献
20.
J.L. Muñoz-Muñoz J.R. Acosta-Motos F. Garcia-Molina R. Varon P.A. Garcia-Ruíz J. Tudela F. Garcia-Cánovas J.N. Rodríguez-López 《Biochimica et Biophysica Acta - Proteins and Proteomics》2010,1804(7):1467-1475
Under aerobic or anaerobic conditions, tyrosinase undergoes a process of irreversible inactivation induced by its physiological substrate l-dopa. Under aerobic conditions, this inactivation occurs through a process of suicide inactivation involving the form oxy-tyrosinase. Under anaerobic conditions, both the met- and deoxy-tyrosinase forms undergo irreversible inactivation. Suicide inactivation in aerobic conditions is slower than the irreversible inactivation under anaerobic conditions. The enzyme has less affinity for the isomer d-dopa than for l-dopa but the velocity of inactivation is the same. We propose mechanisms to explain these processes. 相似文献