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1.
Two crystal forms of lysozyme from the egg white of the embden goose (Anser anser) have been obtained, both of which are suitable for X-ray diffraction analysis. The monoclinic form has space group P21 with cell dimensions a = 38.3 A?, b = 65.7 A?, c = 45.2 a?, β = 116 ° and the triclinic form (space group P1) has cell dimensions of a = 39.9 A?, b = 42.2 A?, c = 57.9 A? and α = 98.8 °, β = 102.5 °, γ = 90.5 °.  相似文献   

2.
A hexagonal crystal form (P6322, a = b = 34.0 A?, c = 113.5 A?) and a monoclinic form (P21, a = 37.1 A?, b = 32.2 A?, c = 32.4 A?, β = 110 °) of neutrophil cationic protein NP2, isolated from rabbit leukocytes, have been characterized. The monoclinic form, containing two promoters (Mr = 3844) per asymmetric unit, diffracts to at least 1.8 Å and is suitable for high-resolution structural studies.  相似文献   

3.
Uteroglobin, which is a progesterone-binding protein of the rabbit uterine secretion, has been crystallized and subjected to X-ray diffraction analysis. Two crystalline forms have been observed: a triclinic one (P1, Z = 2, a = 36.36 (4) A?, b = 37.40 (4) A?, c = 53.28 (4) A?, α = 104.6 (1) °, β = 97.0 (1) °, γ = 111.3(1) °); and an orthorhombic one for which the cell is C-centred with a = 50.86 (5)A?, b = 52.22 (5)A?, c = 47.28 (5) A?, space group C2221, Z = 4. Three isomorphous derivatives have been obtained. The crystals appear suitable for detailed study of the three-dimensional structure of the protein.  相似文献   

4.
Macromomycin (Mr 12,000) is the apoprotein of the antitumor drug auromomycin, which inhibits DNA synthesis by causing single-strand breaks in DNA. Two orthorhombic crystal forms of macromomycin have been observed. The platelike crystals of one form belong to space group P212121, with cell dimensions a = 48·92 A?, b = 54·71 A?, c = 103·31 A?, Z = 8. The crystals of the second form are needle-shaped, and belong to space group P21212, with cell dimensions a = 46·1 A?, b = 54·4 A?, c = 41·2 A?, Z = 4. At this point in time, the platelike crystals appear the most suitable for continued crystallographic studies.  相似文献   

5.
Crystals of the periplasmic ribose binding protein of Salmonella typhimurium have been subjected to X-ray analysis. The crystals grow as rectangular parallelopipeds with the symmetry of space group P21. Unit cell dimensions are a = 64·4 A?, b = 60·6 A?, c = 62·8 A?, and β = 91·25 °. There are two molecules of molecular weight 29,000 per asymmetric unit.  相似文献   

6.
Wheat germ agglutinin crystallizes in two monoclinic space groups, P21 and C2, under identical crystallization conditions. Unit cell dimensions are a = 73.8 A?, b = 51.2 A?, c = 90.8 A?, γ = 90 ° for P21; a = 51.31 A?, b = 73.35 A?, c = 91.45 A?, β = 97.75 ° for C2, both with eight subunit molecules in the unit cell. The C2 crystals were chosen as suitable for investigating the three-dimensional structure to high resolution, because of their smaller asymmetric unit (containing the dimer), and also because they display better diffraction patterns.  相似文献   

7.
The lectin from the seeds of Abrus precatorius has been crystallized and the crystals subjected to study by X-ray diffraction and electron microscopy. Three closely related crystal forms were obtained, of orthorhombic space group P212121 with a = 138 A?, b = 142 A?, and c = 173 A?, of tetragonal space group P41212 with a = b = 136 A?, c = 176 A?, and a twinned intermediate of the first two. From electron microscopy and two-dimensional spatial filtering of electron micrographs of the crystals, the molecule appears to consist of four similar domains grouped in a roughly planar diamond-shaped arrangement having a local intramolecular dyad axis. The average diameter of the Abrus lectin molecule is 50 to 60 Å and the individual domains appear to have a diameter of about 25 Å.  相似文献   

8.
The β-lactamase (penicillinase) from Escherichia coli W3310 has been crystallized from 25% saturated ammonium sulfate solution at pH 6.9. An X-ray examination of the monoclinic crystals shows the space group is C2, with unit cell dimensions a = 47.2 A?, b = 76.9 A?, c = 73.3 A? and β = 98.6°. With Z = 4 and the molecular weight = 22,000 (Melling &; Scott, 1972), the Å3/dalton ratio is 2.98. The crystals are suitable for structure analysis to at least 2.4 Å resolution.  相似文献   

9.
Erysimum latent virus, a tymovirus, contains 180 protein subunits arranged in a T = 3 icosahedral surface lattice. A cubic crystal form (with space group P213 and a = 414 A?) and a monoclinic form (space group B2, a = 442 A?, b = 422 A?, c = 387 A?, γ = 95 °) have been observed. The asymmetric units of the two crystal forms contain one-third and one whole virus particle, respectively. Two possible packing arrangements of the virus particles in the monoclinic unit cell have been deduced from the low-angle diffraction patterns. X-ray diffraction data from the monoclinic crystals extend to at least 3·7 Å resolution.  相似文献   

10.
The Fc fragment was prepared from rabbit immunoglobulin G by digestion with papain, both with the inter-chain disulphide bond intact, and after reduction and alkylation. These two types of Fc crystallized in different, yet related forms, each with one dimer in the asymmetric unit. The covalently linked dimer crystallized in space group P21; a = 68.85 ± 0.05 A?, b = 72.50 ± 0.05 A?, c = 60.40 ± 0.05 A? and β = 105.1 ± 0.2 °. The reduced, non-covalently linked dimer also crystallized in space group P21; a = 81.55 ± 0.05 A?, b = 55.65 ± 0.05 A?, c = 68.85 ± 0.05 A? and β = 1051 ± 0.2 °. A non-crystallographic 2-fold axis relating the two identical polypeptide chains is clearly visible in the h0l projection of the second crystal form.  相似文献   

11.
Binding of a simian virus 40 T antigen-related protein to DNA   总被引:90,自引:0,他引:90  
Crystals of the periplasmic galactose-binding protein of Salmonella typhimurium have been subjected to X-ray analysis. The crystals grow as elongated rectangular prisms, with the symmetry of space group C2. Unit cell dimensions are a = 119·7 A?, b = 37·2 A?, c = 80·1 A?, and β = 123·4. There is one protein molecule of molecular weight 33,000 per asymmetric unit.  相似文献   

12.
Cytochrome c7, a low potential, three heme-containing protein (molecular weight 9800) was isolated from Desulfuromonas acetoxidans and crystallized from polyethylene glycol solutions. The crystals belong to the orthorhombic space group P212121, with unit-cell dimensions a = 44·10 A?, b = 37·55 A? and c = 45·08 A? and have one molecule of protein per asymmetric unit.  相似文献   

13.
The “goose-type” lysozyme isolated from the egg-white of the black swan Cygnus atratus has been crystallized. The space group is P21 with one molecule of protein in the asymmetric unit. The cell parameters are a = 46.2 A?, b = 65.1 A?, c = 38.7 A?, β = 110 °. The crystals diffract to a resolution of 2.25 Å and a set of diffraction data for the native protein has been collected photographically.  相似文献   

14.
A tryptic core (residues 22 to 253) of the single-stranded DNA binding protein, or gene 32 protein, of bacteriophage T4 has been crystallized in four different crystal forms. One of these forms appears suitable for high-resolution X-ray crystallographic studies. It is triclinic, space group PI, with a = 67.7 A?, b = 67.8 A?, c = 66.0 A?, α = 101.6 °, β = 107.0 °, γ = 105.2 °. There appear to be three protein protomers in a near-rhombohedral packing in the unit cell.  相似文献   

15.
Immunity protein, an inhibitor of the ribonuclease activity of the protein antibiotic colicin E3, crystallizes in the orthorhombic space group C222 with cell dimensions a = 78·7 A?, b = 54·1 A?, c = 36·1 A? and one molecule of Mr 9800 per asymmetric unit. The crystals are suitable for high resolution X-ray analysis.  相似文献   

16.
Soybean agglutinin crystallizes in the monoclinic space group C2 with unit cell dimensions a = 118.6 A?, b = 88.9 A?, c = 165.9 A?, β = 103.0 ° and one tetramer of 120,000 Mr per asymmetric unit. The crystals are suitable for high-resolution work.  相似文献   

17.
Crystals of plastocyanins from pea and corn leaves have been obtained. Both are suitable for X-ray structure analysis with a resolution up to 1.8 Å. The crystal form of plastocyanin from pea leaves belongs to the space group P212121 with unit cell dimensions: a = 49.0 A?, b = 53.3 A?, c = 82.6 A?. The assumed number of protein molecules per asymmetric unit of the unit cell is two. Crystals of the oxidized (Cu2+) and reduced (Cu+) forms are isomorphic. No essential differences in spot intensities for the main zone with a resolution of 3 Å were revealed. The crystal form of plastocyanin from corn leaves belongs to the space group P1 with unit cell parameters: a = 24.8 A?, b = 30.0 A?, c = 58.5 A? and α = 96° 10′, β = 87°08′, γ = 78°40′. The assumed number of protein molecules per asymmetric unit is two.  相似文献   

18.
Three crystal forms of rabbit muscle creatine kinase have been grown, one of which seems suited to a high resolution X-ray diffraction study. The first form is of monoclinic space group P21 with a = 54 A?, b = 114 A?, c = 145 A?, β = 91 ° and has as the asymmetric unit two molecules of total molecular weight 160, 000. The second form, grown in the presence of mercurials, is of space group A2 with a = 52 A?, b = 165 A?, c = 237 A?, β = 91 ° and also has two molecules in the asymmetric unit. The third crystal form, grown in the presence of a high concentration of cysteine, is of apparent space group P212121, but evidence indicates that the true space group may be P21221. The dimensions of the orthorhombic unit cell are a = 47 A?, b = 86 A?, c = 125 A?, and the asymmetric unit contains a single protein subunit. Assuming the latter space group, then the creatine kinase molecule possesses a twofold axis relating two identical subunits.  相似文献   

19.
Letter: Packing of microfibrils in collagen   总被引:1,自引:0,他引:1  
X-ray diffraction data obtained from rat tail tendon can be indexed on a tetragonal unit cell with a = 2 × 38·47 A?, c = 4 × 670 A?, space group P41 or P43, in which all the intermicrofibril contacts are quasi-equivalent.  相似文献   

20.
Crystals of mitogenic lectin from pea seeds (Pisum sativum) have been grown. The crystals are in space group P212121 with unit cell dimensions: a = 51.0 ± 0.2 A?, b = 617 ± 0.2 A?, c = 137.6 ± 0.5 A?. The asymmetric unit has one protein molecule.  相似文献   

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