The influence of the mycorrhiza Glomus etunicatum on drought acclimation in safflower and wheat

A study was done to determine the effects of vesicular‐arbuscular mycorrhizal (VAM) colonization on drought acclimation of host plants. Safflower ( Carthamus tinctorius L. cv. S555) and wheat ( Triticum aestivum L. cv. Anza) were grown under environmentally controlled conditions with or without the VAM fungus, Glomus etunicatum Becker and Gerd., and were either acclimated (by pre‐exposing plants to a 10–11 day drought period) or unacclimated to drought. Plants from all treatments were then exposed to drought for 9 days, and plant water status and root water uptake were measured. To minimize interactions between drought and P uptake, growth periods were adjusted so that acclimated and unacclimated plants were similar in size when measurements were made. When wheat was acclimated to drought, osmotic adjustment occurred (leaf solute potentials of leaf tissue rehydrated to full turgor were approximately 0.5 MPa lower in acclimated than unacclimated plants); in safflower, osmotic adjustment was minimal when plants were acclimated. Consequently, acclimated wheat plants were able to tolerate drought better than unacclimated plants, and maintained higher leaf water potentials and relative water contents as soil water was depleted. For both safflower and wheat, acclimated plants had higher water use efficiency, and therefore produced more biomass when water availability was limited, than unacclimated plants. However, mycorrhizal colonization did not affect osmotic adjustment, plant water status, water use efficiency or water uptake in either plant species, and therefore had no effect on drought acclimation or resistance.     

接种菌根真菌对青冈栎幼苗耐旱性的影响

利用丛枝菌根真菌摩西球囊霉(Glomus mosseae)、根内球囊霉(Glomus intraradices)和外生菌根真菌彩色豆马勃(Pisolithus tinctorius)对石漠化地区造林树种青冈栎(Cyclobalanopsis glauca)幼苗进行接种试验。在大棚盆栽条件下模拟土壤干旱胁迫,研究菌根真菌对青冈栎生长和耐旱性的影响。结果表明:在土壤干旱条件下,接种菌根处理植株生物量显著高于未接种处理(P0.05),菌根依赖性随土壤水分含量降低而升高;未接种处理植株叶绿素含量在土壤干旱条件下显著降低(P0.05),除接种Pisolithus tinctorius处理外,其它接种处理叶绿素含量无显著变化。土壤干旱使植株体内脯氨酸和可溶性糖含量上升,在中度干旱条件下,接种处理可溶性糖含量均显著高于对照处理,接种Glomus intraradices、Pisolithus tinctorius处理脯氨酸含量显著低于对照(P0.05);在重度干旱条件下,接种Glomus mosseae和Glomus intraradices处理可溶性糖含量显著高于对照处理(P0.05),而相应的脯氨酸含量显著低于对照处理。当土壤水分含量在田间持水量55%—65%时,接种处理植株SOD、POD和CAT酶活性显著高于未接种处理(P0.05),在土壤水分含量降至35%—45%时,Glomus mosseae和Glomus intraradices处理SOD酶活性显著高于对照,并且所有接种处理POD酶活性均显著高于对照。此外,在水分干旱条件下,植株全磷和全钾含量也显著高于未接种处理(P0.05)。研究表明,丛枝菌根真菌和外生菌根真菌均能够侵染青冈栎幼苗根系;在干旱胁迫条件下,接种菌根真菌能够提高青冈栎植株生物量、抗氧化酶活性、增加植株可溶性糖含量和促进植株养分吸收,提高植株耐旱性,从而使青冈栎幼苗在岩溶干旱环境下更容易存活。     

丛枝菌根提高宿主植物抗旱性分子机制研究进展

丛枝菌根(arbuscular mycorrhiza, AM)对于植物适应各种逆境胁迫具有重要生态学意义。有关菌根共生体对植物抵御干旱胁迫的积极作用已有较多文献报道:无论在植物个体层面——AM调节植物水分生理,还是在生态层面——干旱条件下菌根真菌和宿主植物之间的互动关系,人们都已有一定的认识。然而,目前对于菌根植物适应干旱胁迫的生理和分子机制还缺乏系统深入的研究。综述了近年来相关研究成果,从干旱胁迫相关植物基因入手,讨论了AM对晚期胚胎富集蛋白(LEA)、脯氨酸合成限速酶△¹-吡咯啉-5-羧酸合成酶(P5CS)、水孔蛋白(MIPs),及脱落酸合成途径重要酶9-顺式-环氧类胡萝卜素双加氧酶(NCED)编码基因的可能调控机制,旨在揭示AM共生体提高植物抗旱性的分子基础和实质贡献,同时通过分析当前研究工作薄弱之处及未来研究热点,期望推动相关研究进展。     

丛枝菌根真菌对小麦生长的影响

为了促进经济作物小麦的生长,提高土壤氮磷循环与转化效率,选择两种优良丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)——摩西球囊霉(GM,Glomus mosseae)、根内球囊霉(GI,Glomus intraradices),研究AMF在小麦整个营养生长阶段中对其生长以及对土壤中植物生长需求的大量元素——氮、磷的作用及影响。结果表明:人工施加菌剂可显著提高AMF对小麦的侵染率,施加GM菌剂时,小麦侵染率提高24.54%,同时,株高提高14.08%,小麦地上生物量提高24.05%。GM效果优于GI。施加菌剂后,小麦侵染率与土壤中水解性氮呈显著正相关;植物地上生物量与土壤中总氮,水解性氮呈显著负相关。表明AMF可活化土壤中的氮元素,同时促进作物生长,强化对土壤中氮元素的利用。     

保水剂和丛枝菌根真菌异形根孢囊霉对紫花苜蓿生长与抗旱性的影响

为探讨保水剂（super absorbent polymers,SAP）和丛枝菌根真菌（arbuscular mycorrhizal fungi,AMF）对植物生长和抗旱性的影响,以紫花苜蓿Medicago sativa为供试植物,开展了温室盆栽试验。植物播种时设置土壤添加和不添加聚丙烯酰胺型SAP（BJ2101）处理,以及接种和不接种异形根孢囊霉Rhizophagus irregularis处理,通过称重法维持12%的土壤含水量（正常供水）,植物生长30d,各处理一半植物接受干旱胁迫（6%的土壤含水量）,另一半仍正常供水,持续30d后收获。结果表明,在干旱胁迫下,接种AMF显著增加了紫花苜蓿的植株干重,促进了植物对矿质元素的吸收,提高了叶片中叶绿素和脯氨酸含量,增强了植物的抗旱性。SAP抑制了R. irregularis对植物根系的侵染;与单接种AMF相比,SAP和AMF的联合施用降低了紫花苜蓿的生物量,影响了植物对矿质元素的吸收。本研究中,SAP和AMF的联合施用并没有表现出协同增效作用,这一方面可能是因为研究设定的土壤水分管理模式,另一方面SAP与AMF共同施用的适宜条件还需进一步探索优化。     

丛枝菌根真菌对植物耐旱性的影响研究进展

丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)能与植物根系形成互惠共生体,对植物的生长发育和抗逆性有积极的影响,在改善植物水分代谢和提高植物耐旱性中发挥了重要作用.本文综述了近年来AMF与植物水分代谢关系的研究进展,从植物的光合作用、蒸腾与气孔导度、水分利用效率、水力导度、渗透调节、内源激素和抗氧化系统等方面说明AMF对植物水分代谢的影响.从4个方面介绍了AMF提高植物耐旱性的机理:1)菌丝网络增加植物根系吸收范围;2)增强植物保水能力和抗氧化能力;3)稳定和改善土壤团聚体;4)促进植物养分吸收.并提出今后研究需注意的问题和建议.     

Interaction of drought and high temperature on photosynthesis and grain-filling of wheat

Drought and high temperature often occur simultaneously, but their effects on crops are usually investigated individually. Our objective was to compare effects of drought, high temperature, and their interactions on photosynthesis and grain-growth of wheat (Triticum aestivum L.). Plants (cv. Len) were grown uniformly in well-watered soil at 25/20 ± 2 °C day/night until anthesis, when they were subjected to regimes of no drought (soil at field capacity) and drought (plant water potential of –.0 to –2.4 MPa) at 15/10, 25/20, and 35/30 °C in controlled environments until physiological maturity. Drought decreased photosynthesis, stomatal conductance, viable leaf area, shoot and grain mass, and weight and soluble sugar content of kernels but increased plant water-use efficiency. High temperature hastened the decline in photosynthesis and leaf area, decreased shoot and grain mass as well as weight and sugar content of kernels, and reduced water-use efficiency. Interactions between the two stresses were pronounced, and consequences of drought on all physiological parameters were more severe at high temperature than low temperature. The synergistic interactions indicated that productivity of wheat is reduced considerably more by the combined stresses than by either stress alone, and that much of the effect is on photosynthetic processes.     

Effects of drought, transgenic expression of a fructan synthesizing enzyme and of mycorrhizal symbiosis on growth and soluble carbohydrate pools in tobacco plants

The effects of three conditions likely to affect soluble carbohydrate pools, namely drought, expression of barley sucrose: fructan 6-fructosyl transferase (6-SFT, EC 2.4.1.10) and the establishment of the arbuscular mycorrhizal symbiosis with Glomus mosseae were studied in a multifactorial experiment using tobacco ( Nicotiana tabacum ). Tobacco, a plant naturally unable to form fructan, accumulated fructan in leaves, and to a larger extent in the roots, when transformed with 6-SFT. Under drought conditions, growth was considerably reduced, but neither expression of 6-SFT nor mycorrhiza formation had an effect on growth rate. However, in response to drought, carbon partitioning was significantly altered towards accumulation of soluble sugars. In plants exposed to drought, pools of sucrose were greater than those of unstressed plants, particularly in their roots. In the transgenic plants expressing 6-SFT, there were also increased contents of the products of 6-SFT, namely fructan, most probably because of the increased availability of the substrate, sucrose. These effects were the same in the presence or absence of mycorrhiza. Hexoses (glucose and fructose) also increased in response to drought, primarily in the leaves. This effect of drought was little affected by the expression of 6-SFT, except that it slightly enhanced drought-induced glucose accumulation in roots. However, the presence of mycorrhiza led to a considerable reduction in drought-induced accumulation of hexoses in the leaves. The content of the fungal disaccharide trehalose was greatly increased in the roots of all mycorrhizal plants upon exposure to drought, particularly in some of the transgenic plants expressing 6-SFT.     

Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Cinnamomum migao by enhancing physio‐biochemical responses

Drought is the main limiting factor for plant growth in karst areas with a fragile ecological environment. Cinnamomum migao H.W. Li is an endemic medicinal woody plant present in the karst areas of southwestern China, and it is endangered due to poor drought tolerance. Arbuscular mycorrhizal fungi (AMF) are known to enhance the drought tolerance of plants. However, few studies have examined the contribution of AMF in improving the drought tolerance of C. migao seedlings. Therefore, we conducted a series of experiments to determine whether a single inoculation and coinoculation of AMF (Claroideoglomus lamellosum and Claroideoglomus etunicatum) enhanced the drought tolerance of C. migao. Furthermore, we compared the effects of single inoculation and coinoculation with different inoculum sizes (20, 40, 60, and 100 g; four replicates per treatment) on mycorrhizal colonization rate, plant growth, photosynthetic parameters, antioxidant enzyme activity, and malondialdehyde (MDA) and osmoregulatory substance contents. The results showed that compared with nonmycorrhizal plants, AMF colonization significantly improved plant growing status; net photosynthetic rate; superoxide dismutase, catalase, and peroxidase activities; and soluble sugar, soluble protein, and proline contents. Furthermore, AMF colonization increased relative water content and reduced MDA content in cells. These combined cumulative effects of AMF symbiosis ultimately enhanced the drought tolerance of seedlings and were closely related to the inoculum size. With an increase in inoculum size, the growth rate and drought tolerance of plants first increased and then decreased. The damage caused by drought stress could be reduced by inoculating 40–60 g of AMF, and the effect of coinoculation was significantly better than that of single inoculation at 60 g of AMF, while the effect was opposite at 40 g of AMF. Additionally, the interaction between AMF and inoculum sizes had a significant effect on drought tolerance. In conclusion, the inoculation of the AMF (Cl. lamellosum and Cl. etunicatum) improved photosynthesis, activated antioxidant enzymes, regulated cell osmotic state, and enhanced the drought tolerance of C. migao, enabling its growth in fragile ecological environments.     

丛枝菌根真菌通过上调根系及自身水孔蛋白基因表达提高玉米抗旱性

在模拟干旱条件下, 研究了接种丛枝菌根(AM)真菌Glomus intraradices对玉米(Zea mays)根部13种质膜水孔蛋白基因表达的影响, 同时观测了AM真菌自身水孔蛋白基因的表达情况。结果表明, 干旱条件下, 除Zm PIP1;3、Zm PIP1;4、Zm PIP1;5和Zm PIP2;2之外的接种处理能显著提高根部其他8种质膜水孔蛋白基因的表达(Zm PIP2;7表达量未检测出), 并且AM真菌菌丝中水孔蛋白基因GintAQP1表达也显著增强。与此同时, 接种处理明显改善了植物水分状况, 提高了叶片水势。AM真菌增强宿主植物根部及自身的水孔蛋白基因的表达对于提高植物抗旱性具有潜在的重要贡献。     

丛枝菌根真菌对杨树生长、气孔和木质部微观结构的影响

植物气孔与木质部导管及纤维的功能直接关系着植物的水分利用, 进而影响植物的生长。为研究丛枝菌根真菌(AMF)对杨树抗旱性的影响, 采用温室盆栽的方法, 研究两种水分条件下, 接种根内球囊霉(Rhizophagus irregularis)对速生杨107 Populus × canadensis (P. nigra × P. deltoides) ‘Neva’气孔及木质部微观结构的影响。结果表明: AMF的侵染显著提高了杨树幼苗地上和地下部分生物量, 对叶片气孔长度、茎部导管细胞直径和纤维细胞长度也有促进作用。AMF对生物量和导管细胞直径的增加幅度表现出干旱条件下>正常水分条件下, 而对气孔长度的提高幅度表现出干旱条件下<正常水分条件下。正常水分条件下, AMF增加了杨树叶片的气孔密度, 减小了纤维细胞直径, 对相对水分饱和亏缺无影响; 干旱条件下, AMF增加了纤维细胞直径, 降低了相对水分饱和亏缺, 对气孔密度无影响。综上所述, 干旱条件下, AMF对导管水分传输能力的促进作用明显增加, 而对气孔蒸腾能力的促进作用有所减少, 从而更利于杨树在遭遇干旱时保持水分, 减少干旱对菌根杨树造成的水分亏缺, 提高菌根杨树对干旱的耐受性。     

盐旱复合胁迫对小麦幼苗生长和水分吸收的影响

为明确盐害、干旱及盐旱复合胁迫对小麦幼苗生长和水分吸收的影响,从而为盐害和干旱胁迫下栽培调控提供理论依据。以2个抗旱性不同的小麦品种(扬麦16和耐旱型洛旱7号)为材料,采用水培试验,以NaCl和PEG模拟盐旱复合胁迫,研究了盐旱复合胁迫下小麦幼苗生长、根系形态、光合特性及水分吸收特性的变化。结果表明,盐、旱及复合胁迫下小麦幼苗的生物量、叶面积、总根长与根系表面积、叶绿素荧光和净光合速率均显著下降,但是复合胁迫处理的降幅却显著低于单一胁迫。盐旱复合胁迫下根系水导速率和根系伤流液强度显著大于单一胁迫,从而提高了小麦幼苗叶片水势和相对含水量。盐胁迫下小麦幼苗Na~+/K~+显著大于复合胁迫,但复合胁迫下ABA含量却显著小于单一的盐害和干旱胁迫。因此,盐旱复合胁迫可以通过增强根系水分吸收及降低根叶中ABA含量以维持较高光合能力,这是盐旱复合胁迫提高小麦适应性的重要原因。洛旱7号和扬麦16对盐及盐旱复合胁迫的响应基本一致,但在干旱胁迫下洛旱7号表现出明显的耐性。     

丛枝菌根真菌影响作物非生物胁迫耐受性的研究进展

土壤中存在着大量不同种类的微生物资源,土壤微生物能够与自然界中的大多数植物密切合作,其中丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)起着十分重要的作用。近年来,对于AMF的研究越来越多。AMF是存在于土壤中的重要真菌之一,是土壤中的菌根真菌菌丝与高等植物营养根系形成的一种联合体。AMF可以与陆地上90%左右的植物根系形成共生体,通过在植物根系形成重要的“丛枝菌根”结构而为植物提供更多的养分。为了了解接种AMF对作物生长过程中耐受一些非生物胁迫(如干旱、极端温度、重金属污染、盐分、不利的土壤pH变化等)性能方面的影响,基于之前接种AMF对养分胁迫下玉米生长影响的研究,在扩大作物品种的基础上,通过查阅大量文献,结合试验研究及对前人和近年来关于AMF的一些最新研究进展,获得了具有实践性意义的新发现：AMF与植物共生有助于植物生长,可以改善植物的营养状况,并且可以保护植物免受各种非生物环境胁迫的影响。由此可以得出结论：AMF通过各种机制改善植物生长状况,提高作物抗逆性,为作物增产、农民增收创造了福利,并且避免了由于肥料过量施用导致的一些污染环境问题。本文主要综述了接种AMF在各种非生物环境胁迫(干旱、极端温度、重金属污染、盐分、不利的土壤pH变化等)条件下对植株生长和发育的有利影响,并对目前存在的不足和今后研究的重点提出几点建议。     

干旱条件下AM真菌对植物生长和土壤水稳定性团聚体的影响

采用分室培养系统,模拟正常水分和干旱胁迫两种环境条件,探讨不同丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)对紫花苜蓿(Medicago sativa L.)生长和土壤水稳性团聚体的影响.试验条件下,Glomus intraradices对苜蓿根系的侵染率均显著高于Acaulospora scrobiculata和Diversispora spurcum接种处理.正常水分条件下,供试AM真菌均能显著提高植株生物量及磷浓度.干旱胁迫显著抑制了植株生长和菌根共生体发育,总体上菌根共生体对植株生长没有明显影响,接种D.spurcum甚至趋于降低植株生物量;同时,仅有G.intraradices显著提高了植株磷浓度.AM真菌主要影响到＞2mm的水稳性团聚体数量,以G.intraradices作用效果最为显著.在菌丝室中,G.intraradices显著提高了总球囊霉素含量.研究表明AM真菌对土壤大团聚体形成具有积极作用,而菌根效应因土壤水分条件和不同菌种而异,干旱胁迫下仅有G.intraradices对土壤结构和植物生长表现出显著积极作用.在应用菌根技术治理退化土壤时,需要选用抗逆性强共生效率高的菌株,对于不同AM真菌抗逆性差异的生物学与遗传学基础尚需进一步研究.     

丛枝菌根真菌对薰衣草耐热性的影响

于不同温度（25℃/20℃、35℃/30℃和40℃/35℃）下测定接种丛枝菌根真菌（arbuscular mycorrhizal fungi,AMF）摩西斗管囊霉Funneliformis mosseae、变形球囊霉Glomus versiforme和F. mosseae+G. versiforme 混合菌种处理对狭叶薰衣草Lavandula angustifolia耐热性的影响。结果表明,供试AMF能与狭叶薰衣草根系形成菌根共生体,以混合菌种处理的侵染率最高,达到68%。40℃/35℃下,与不接种AMF对照相比,混合菌种处理的狭叶薰衣草叶片可溶性糖、可溶性蛋白、脯氨酸和叶绿素等含量以及根系活力分别提高了46%、68%、65%、29%和70%;与不接种AMF对照相比,3种温度处理下接种AMF显著增加了狭叶薰衣草植株超氧化物歧化酶、过氧化物酶、过氧化氢酶、抗坏血酸过氧化物酶和硝酸还原酶活性,而降低相对电导率及丙二醛含量。表明接种AMF能增强狭叶薰衣草抗氧化酶活性,减轻高温造成的伤害,增强耐热性,与单一接种相比以混合接种摩西斗管囊霉和变形球囊霉提高狭叶薰衣草耐热性的效应最大。     

不同干旱胁迫条件下丛枝菌根真菌对 木棉叶绿素荧光参数的影响

为了探明丛枝菌根真菌(AMF)与木棉(Bombax ceiba Linn.)抗旱性的关系,采用盆栽法对不同干旱胁迫条件下接菌组(1 g土壤含100个AMF孢子)和未接菌组木棉叶绿素荧光参数进行了比较分析.结果表明:中度干旱胁迫(土壤相对含水量45%~50%)和重度干旱胁迫(土壤相对含水量30%~35%)条件下接菌组的初始荧光(Fo)均低于未接菌组;与对照(土壤相对含水量65%~70%)相比,重度干旱胁迫5、15和35 d,接菌组的Fo值分别升高了10.39%、20.93%和14.14%,未接菌组的Fo值分别升高了26.95%、48.75%和71.35%.接菌组的PSⅡ最大光化学效率(Fv/Fm)和PSⅡ潜在活性(Fv/Fo)在中度和重度干旱胁迫5、15和35 d均高于未接菌组.与对照相比,重度干旱胁迫5、15和35 d,接菌组的Fv/Fm值分别降低了9.09%、6.85%和22.06%,未接菌组的Fv/Fm值分别降低了10.67%、16.21%和52.78%;接菌组的Fv/Fo值分别降低了28.14%、22.43%和46.01%,未接菌组的Fv/Fo值分别降低了28.38%、48.20%和75.96%.总体来看,在中度和重度干旱胁迫条件下,接菌组的PSⅡ实际光量子产量〔Y(Ⅱ)〕、PSⅡ非调节性能量耗散的量子产量〔Y(NO)〕、光化学淬灭系数(qP)和光合电子传递速率(ETR)高于对照,而PSⅡ调节性能量耗散的量子产量〔Y(NPQ)〕和非光化学淬灭系数(qN)低于对照;未接菌组的Y(Ⅱ)、qP、qN和ETR值低于对照,而Y(NO)和Y(NPQ)值则高于对照.研究结果显示:AMF能够减轻干旱尤其是重度干旱胁迫条件下木棉受伤害和光抑制的程度,并提高其叶片PSⅡ反应中心的活性,从而增强木棉在干旱胁迫条件下的生存能力.     

干旱胁迫下AMF对云南蓝果树幼苗生长和光合特征的影响

采用盆栽试验与称重控水法,将土壤相对含水量分别控制在田间最大持水量的100%、91.68%、82.85%、60.00%、41.86%和21.28%,并在这6个不同的土壤相对含水量条件下,分别设添加苯菌灵(杀真菌剂)(低AMF)和不添加苯菌灵(高AMF)处理,研究干旱胁迫下AMF对极小种群野生植物云南蓝果树幼苗生长和光合特征的影响,揭示云南蓝果树濒危的微生物学机制,为云南蓝果树保护措施的制定与实施奠定基础。结果表明,添加苯菌灵处理显著降低了不同水分条件下的AMF侵染率,说明试验中AMF处理的实生苗在生长和光合特征上的差异是苯菌灵处理下侵染率下降导致的;随着干旱胁迫的加剧,云南蓝果树幼苗的根部AMF侵染率显著降低、叶面积等生长指标和净光合速率(Pn)等光合参数都发生显著变化;高AMF处理可以显著增加水分充足和轻度干旱胁迫条件下云南蓝果树幼苗的大部分生长指标和光合参数,而对重度胁迫下的云南蓝果树幼苗没有显著影响,说明重度干旱胁迫对其影响大于AMF的影响;另外,整合了可塑性指数分析和隶属函数分析两种方法对其抗旱性进行评价,云南蓝果树幼苗基本上无法通过调节形态和光合能力来适应水分环境的变化,但是高AMF处理可使云南蓝果树幼苗具有较强的可塑性和更强的抗旱性。实验结果为云南蓝果树的科学保育及种苗繁育提供了理论依据。     

干旱胁迫下接种丛枝菌根真菌对七子花非结构性碳水化合物积累及C、N、P化学计量特征的影响

以濒危植物七子花二年生幼苗为研究材料,采用盆栽试验方法,研究干旱胁迫和接种丛枝菌根真菌(AMF)处理对幼苗不同器官C、N、P化学计量关系和非结构性碳水化合物(NSC)含量的影响。试验共设计4个处理:对照(CK)、干旱胁迫(D)、接种AMF(AMF)、干旱胁迫和接种AMF(D+AMF)。结果表明: 在干旱胁迫下七子花根系AMF的侵染率显著下降,但接种AMF处理植株的株高、叶片数显著高于未接种处理。接种AMF显著提高了干旱胁迫下植株根、叶可溶性糖和NSC含量及茎、叶淀粉含量,且茎和叶可溶性糖与淀粉比显著下降。干旱胁迫导致植株C含量在根和叶中显著增加,P含量在茎中显著减少;与干旱胁迫相比,胁迫下接种AMF植株根、茎、叶P含量及叶C含量显著提高,而根C、N含量及茎C含量显著降低。胁迫下接种AMF植株根、茎C∶N、C∶P、N∶P和叶N∶P均显著低于单一胁迫处理。NSC与C∶N∶P计量比的相关性分析表明,根、叶P含量与可溶性糖和NSC含量呈显著正相关,茎P含量与淀粉和NSC含量呈显著正相关,各器官N∶P与NSC含量呈显著负相关。综上,干旱胁迫显著抑制了七子花幼苗的生长,接种AMF通过提高植株根和叶的可溶性糖含量、根的可溶性糖/淀粉,增加地上部分淀粉含量,促进P元素吸收和降低各器官N∶P来增强植株耐旱性,从而提高七子花幼苗在干旱环境中的存活率。