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Faithful initiation of ribosomal RNA transcription from cloned DNA by purified RNA polymerase I 总被引:8,自引:0,他引:8
M R Paule C T Iida P J Perna G H Harris S L Brown Shimer P Kownin 《Biochemistry》1984,23(18):4167-4172
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Drosophila RNA polymerases I &; II were used to transcribe a recombinant bacterial plasmid containing one copy of Drosophila ribosomal DNA. Both supercoiled and relaxed, closed circular plasmids were used. With Mg+2 as the divalent cation, enzyme I is much more active on both forms of the plasmid; the relaxed form in particular supports almost no RNA synthesis by enzyme II. When Mn+2 is present, differences in template efficiencies are minimal. The differences observed in the absence of Mn+2 seem to depend only on different preferences for the physical state of the template and not on recognition of specific promotor sequences, since enzyme I shows no strand selection when transcribing these plasmids. 相似文献