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1.
Tsukasa Nunome Satomi Negoro Izumi Kono Hiroyuki Kanamori Koji Miyatake Hirotaka Yamaguchi Akio Ohyama Hiroyuki Fukuoka 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(6):1143-1153
Eggplant (Solanum melongena L.), also known as aubergine or brinjal, is an important vegetable in many countries. Few useful molecular markers have been
reported for eggplant. We constructed simple sequence repeat (SSR)-enriched genomic libraries in order to develop SSR markers,
and sequenced more than 14,000 clones. From these sequences, we designed 2,265 primer pairs to flank SSR motifs. We identified
1,054 SSR markers from amplification of 1,399 randomly selected primer pairs. The markers have an average polymorphic information
content of 0.27 among eight lines of S. melongena. Of the 1,054 SSR markers, 214 segregated in an intraspecific mapping population. We constructed cDNA libraries from several
eggplant tissues and obtained 6,144 expressed sequence tag (EST) sequences. From these sequences, we designed 209 primer pairs,
7 of which segregated in the mapping population. On the basis of the segregation data, we constructed a linkage map, and mapped
the 236 segregating markers to 14 linkage groups. The linkage map spans a total length of 959.1 cM, with an average marker
distance of 4.3 cM. The markers should be a useful resource for qualitative and quantitative trait mapping and for marker-assisted
selection in eggplant breeding. 相似文献
2.
Jones S Dupal P Dumsday L Hughes J Forster W 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,105(4):577-584
A simple sequence repeat (SSR)-based linkage map has been constructed for perennial ryegrass (Lolium perenne L.) using a one-way pseudo-testcross reference population. A total of 309 unique perennial ryegrass SSR (LPSSR) primer pairs showing efficient amplification were evaluated for genetic polymorphism, with 31% detecting segregating alleles. Ninety-three loci have been assigned to positions on seven linkage groups. The majority of the mapped loci are derived from cloned sequences containing (CA)n-type dinucleotide SSR arrays. A small number (7%) of primer pairs amplified fragments that mapped to more than one locus. The SSR locus data has been integrated with selected data for RFLP, AFLP and other loci mapped in the same population to produce a composite map containing 258 loci. The SSR loci cover 54% of the genetic map and show significant clustering around putative centromeric regions. BLASTN and BLASTX analysis of the sequences flanking mapped SSRs indicated that a majority (84%) are derived from non-genic sequences, with a small proportion corresponding to either known repetitive DNA sequence families or predicted genes. The mapped LPSSR loci provide the basis for linkage group assignment across multiple mapping populations. 相似文献
3.
Torben Asp Ursula K Frei Thomas Didion Klaus K Nielsen Thomas Lübberstedt 《BMC plant biology》2007,7(1):36
Background
Simple sequence repeat (SSR) markers are highly informative and widely used for genetic and breeding studies in several plant species. They are used for cultivar identification, variety protection, as anchor markers in genetic mapping, and in marker-assisted breeding. Currently, a limited number of SSR markers are publicly available for perennial ryegrass (Lolium perenne). We report on the exploitation of a comprehensive EST collection in L. perenne for SSR identification. The objectives of this study were 1) to analyse the frequency, type, and distribution of SSR motifs in ESTs derived from three genotypes of L. perenne, 2) to perform a comparative analysis of SSR motif polymorphisms between allelic sequences, 3) to conduct a comparative analysis of SSR motif polymorphisms between orthologous sequences of L. perenne, Festuca arundinacea, Brachypodium distachyon, and O. sativa, 4) to identify functionally associated EST-SSR markers for application in comparative genomics and breeding. 相似文献4.
Chidananda Nagamangala Kanchiswamy Hirotaka Takahashi Stefano Quadro Massimo E Maffei Simone Bossi Cinzia Bertea Simon Atsbaha Zebelo Atsushi Muroi Nobuaki Ishihama Hirofumi Yoshioka Wilhelm Boland Junji Takabayashi Yaeta Endo Tatsuya Sawasaki Gen-ichiro Arimura 《BMC plant biology》2010,10(1):1-10
Background
Genetic markers and linkage mapping are basic prerequisites for marker-assisted selection and map-based cloning. In the case of the key grassland species Lolium spp., numerous mapping populations have been developed and characterised for various traits. Although some genetic linkage maps of these populations have been aligned with each other using publicly available DNA markers, the number of common markers among genetic maps is still low, limiting the ability to compare candidate gene and QTL locations across germplasm.Results
A set of 204 expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers has been assigned to map positions using eight different ryegrass mapping populations. Marker properties of a subset of 64 EST-SSRs were assessed in six to eight individuals of each mapping population and revealed 83% of the markers to be polymorphic in at least one population and an average number of alleles of 4.88. EST-SSR markers polymorphic in multiple populations served as anchor markers and allowed the construction of the first comprehensive consensus map for ryegrass. The integrated map was complemented with 97 SSRs from previously published linkage maps and finally contained 284 EST-derived and genomic SSR markers. The total map length was 742 centiMorgan (cM), ranging for individual chromosomes from 70 cM of linkage group (LG) 6 to 171 cM of LG 2.Conclusions
The consensus linkage map for ryegrass based on eight mapping populations and constructed using a large set of publicly available Lolium EST-SSRs mapped for the first time together with previously mapped SSR markers will allow for consolidating existing mapping and QTL information in ryegrass. Map and markers presented here will prove to be an asset in the development for both molecular breeding of ryegrass as well as comparative genetics and genomics within grass species. 相似文献5.
Development and mapping of SSR markers for maize 总被引:45,自引:0,他引:45
Sharopova N McMullen MD Schultz L Schroeder S Sanchez-Villeda H Gardiner J Bergstrom D Houchins K Melia-Hancock S Musket T Duru N Polacco M Edwards K Ruff T Register JC Brouwer C Thompson R Velasco R Chin E Lee M Woodman-Clikeman W Long MJ Liscum E Cone K Davis G Coe EH 《Plant molecular biology》2002,48(5-6):463-481
Microsatellite or simple sequence repeat (SSR) markers have wide applicability for genetic analysis in crop plant improvement strategies. The objectives of this project were to isolate, characterize, and map a comprehensive set of SSR markers for maize (Zea mays L.). We developed 1051 novel SSR markers for maize from microsatellite-enriched libraries and by identification of microsatellite-containing sequences in public and private databases. Three mapping populations were used to derive map positions for 978 of these markers. The main mapping population was the intermated B73 × Mo17 (IBM) population. In mapping this intermated recombinant inbred line population, we have contributed to development of a new high-resolution map resource for maize. The primer sequences, original sequence sources, data on polymorphisms across 11 inbred lines, and map positions have been integrated with information on other public SSR markers and released through MaizeDB at URL:www.agron.missouri.edu. The maize research community now has the most detailed and comprehensive SSR marker set of any plant species. 相似文献
6.
Birte Pakull Katrin Groppe Matthias Meyer Torsten Markussen Matthias Fladung 《Tree Genetics & Genomes》2009,5(3):505-515
A large number of simple sequence repeat (SSR) marker-containing genetic maps are available for several Populus species. For aspen however, no SSR-containing map has been published so far. In this study, genetic linkage mapping was carried
out with an interspecific mapping pedigree of 61 full-sib hybrids of European × quaking aspen (Populus tremula L. × Populus tremuloides Michx.), using the two-way pseudo-testcross strategy. Amplified fragment-length polymorphism (AFLP) and SSR markers were
used for mapping, resulting in the first SSR-containing genetic linkage maps for aspen. The maps allow comparisons with a
Populus consensus map and other published genetic maps of the genus Populus. The maps showed good collinearity to each other and to the Populus consensus map and provide a direct link to the Populus trichocarpa genomic sequence. Sex as a morphological trait was assessed in the mapping population and mapped on a non-terminal position
of linkage group XIX on the male P. tremuloides map. 相似文献
7.
Yanbin Hong Xiaoping Chen Xuanqiang Liang Haiyan Liu Guiyuan Zhou Shaoxiong Li Shijie Wen C Corley Holbrook Baozhu Guo 《BMC plant biology》2010,10(1):17
Background
The construction of genetic linkage maps for cultivated peanut (Arachis hypogaea L.) has and continues to be an important research goal to facilitate quantitative trait locus (QTL) analysis and gene tagging for use in a marker-assisted selection in breeding. Even though a few maps have been developed, they were constructed using diploid or interspecific tetraploid populations. The most recently published intra-specific map was constructed from the cross of cultivated peanuts, in which only 135 simple sequence repeat (SSR) markers were sparsely populated in 22 linkage groups. The more detailed linkage map with sufficient markers is necessary to be feasible for QTL identification and marker-assisted selection. The objective of this study was to construct a genetic linkage map of cultivated peanut using simple sequence repeat (SSR) markers derived primarily from peanut genomic sequences, expressed sequence tags (ESTs), and by "data mining" sequences released in GenBank. 相似文献8.
Bruno Studer Torben Asp Ursula Frei Stephan Hentrup Helena Meally Aurélie Guillard Susanne Barth Hilde Muylle Isabel Roldán-Ruiz Philippe Barre Carole Koning-Boucoiran Gerda Uenk-Stunnenberg Oene Dolstra Leif Skøt Kirsten P. Skøt Lesley B. Turner Mervyn O. Humphreys Roland Kölliker Niels Roulund Klaus K. Nielsen Thomas Lübberstedt 《Molecular breeding : new strategies in plant improvement》2008,21(4):533-548
An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing
ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred
and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes
included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer
sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding
and comparative mapping within and among forage and turf grasses.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
9.
Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map 总被引:25,自引:0,他引:25
A. A. Sankar G. A. Moore 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(2-3):206-214
Inter-simple sequence repeat (ISSR) analysis was evaluated for its usefulness in generating markers to extend the genetic
linkage map of Citrus using a backcross population previously mapped with restriction fragment length polymorphism (RFLP), random amplified polymorphic
DNA (RAPD) and isozyme markers. ISSR markers were obtained through the simple technique of PCR followed by analysis on agarose
gels, using simple sequence repeat (SSR) primers. Optimization of reaction conditions was achieved for 50% of the SSR primers
screened, and the primers amplified reproducible polymorphic bands in the parents and progeny of the backcross population.
Mendelian segregation of the polymorphic bands was demonstrated, with an insignificant number of skewed loci. Most of the
SSR primers produced dominant loci; however co-dominance was observed with loci derived from three primers. A new genetic
map was produced by combining the segregation data for the ISSR markers and data for the RFLP, RAPD and isozyme markers from
the previous map and creating genetic linkages among all the markers using JoinMap 2.0 mapping software. The new map has an
improved distribution of markers along the linkage groups with fewer gaps, and marker order showed partial or complete conservation
in the linkage groups. The incorporation of ISSR markers into the genetic linkage map demonstrates that ISSR markers are suitable
for genetic mapping in Citrus.
Received: 3 February 2000 / Accepted: 12 May 2000 相似文献
10.
Warnke SE Barker RE Jung G Sim SC Rouf Mian MA Saha MC Brilman LA Dupal MP Forster JW 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(2):294-304
Annual (Lolium multiflorum Lam.) and perennial (L. perenne L.) ryegrass are two common forage and turfgrass species grown throughout the world. Perennial ryegrass is most commonly used for turfgrass purposes, and contamination by annual ryegrass, through physical seed mixing or gene flow, can result in a significant reduction in turfgrass quality. Seed certifying agencies in the United States currently use a test called seedling root fluorescence (SRF) to detect contamination between these species. The SRF test, however, can be inaccurate and therefore, the development of additional markers for species separation is needed. Male and female molecular-marker linkage maps of an interspecific annual × perennial ryegrass mapping population were developed to determine the map location of the SRF character and to identify additional genomic regions useful for species separation. A total of 235 AFLP markers, 81 RAPD markers, 16 comparative grass RFLPs, 106 SSR markers, 2 isozyme loci and 2 morphological characteristics, 8-h flowering, and SRF were used to construct the maps. RFLP markers from oat and barley and SSR markers from tall fescue and other grasses allowed the linkage groups to be numbered, relative to the Triticeae and the International Lolium Genome Initative reference population P150/112. The three-generation population structure allowed both male and female maps to be constructed. The male and female maps each have seven linkage groups, but differ in map length with the male map being 537 cm long and the female map 712 cm long. Regions of skewed segregation were identified in both maps with linkage groups 1, 3, and 6 of the male map showing the highest percentage of skewed markers. The (SRF) character mapped to linkage group 1 in both the male and female maps, and the 8-h flowering character was also localized to this linkage group on the female map. In addition, the Sod-1 isozyme marker, which can separate annual and perennial ryegrasses, mapped to linkage group 7. These results indicate that Lolium linkage groups 1 and 7 may provide additional markers and candidate genes for use in ryegrass species separation.Communicated by C. Möllers 相似文献
11.
Genetic linkage maps of rose constructed with new microsatellite markers and locating QTL controlling flowering traits 总被引:1,自引:0,他引:1
L. Hibrand-Saint Oyant L. Crespel S. Rajapakse L. Zhang F. Foucher 《Tree Genetics & Genomes》2008,4(1):11-23
New microsatellites markers [simple sequence repeat (SSR)] have been isolated from rose and integrated into an existing amplified
fragment-length polymorphism genetic map. This new map was used to identify quantitative trait locus (QTL) controlling date
of flowering and number of petals. From a rose bud expressed sequence tag (EST) database of 2,556 unigenes and a rose genomic
library, 44 EST-SSRs and 20 genomic-SSR markers were developed, respectively. These new rose SSRs were used to expand genetic
maps of the rose interspecific F1 progeny. In addition, SSRs from other Rosaceae genera were also tested in the mapping progeny. Genetic maps for the two parents of the progeny were constructed using pseudo-testcross
mapping strategy. The maps consist of seven linkage groups of 105 markers covering 432 cM for the maternal map and 136 markers
covering 438 cM for the paternal map. Homologous relationships among linkage groups between the maternal and paternal maps
were established using SSR markers. Loci controlling flowering traits were localised on genetic maps as a major gene and QTL
for the number of petals and a QTL for the blooming date. New SSR markers developed in this study will provide tools for the
establishment of a consensus linkage map for roses that combine traits and markers in various rose genetic maps. 相似文献
12.
Truco MJ Antonise R Lavelle D Ochoa O Kozik A Witsenboer H Fort SB Jeuken MJ Kesseli RV Lindhout P Michelmore RW Peleman J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(6):735-746
An integrated map for lettuce comprising of 2,744 markers was developed from seven intra- and inter-specific mapping populations.
A total of 560 markers that segregated in two or more populations were used to align the individual maps. 2,073 AFLP, 152
RFLP, 130 SSR, and 360 RAPD as well as 29 other markers were assigned to nine chromosomal linkage groups that spanned a total
of 1,505 cM and ranged from 136 to 238 cM. The maximum interval between markers in the integrated map is 43 cM and the mean
interval is 0.7 cM. The majority of markers segregated close to Mendelian expectations in the intra-specific crosses. In the
two L. saligna × L. sativa inter-specific crosses, a total of 155 and 116 markers in 13 regions exhibited significant segregation distortion. Data visualization
tools were developed to curate, display and query the data. The integrated map provides a framework for mapping ESTs in one
core mapping population relative to phenotypes that segregate in other populations. It also provides large numbers of markers
for marker assisted selection, candidate gene identification, and studies of genome evolution in the Compositae.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
13.
SSR-based linkage map with new markers using an intraspecific population of common wheat 总被引:14,自引:0,他引:14
Torada A Koike M Mochida K Ogihara Y 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(6):1042-1051
Simple sequence repeats (SSRs) are valuable molecular markers in many plant species. In common wheat (Triticum aestivum L.), which is characteristic of its large genomes and alloploidy, SSRs are one of the most useful markers. To increase SSR
marker sources and construct an SSR-based linkage map of appropriate density, we tried to develop new SSR markers from SSR-enriched
genomic libraries and the public database. SSRs having (GA)n and (GT)n motifs were isolated from enriched libraries, and di-
and tri-nucleotide repeats were mined from expressed sequence tags (ESTs) and DNA sequences of Triticum species in the public database. Of the 1,147 primer pairs designed, 842 primers gave accurate amplification products, and
478 primers showed polymorphism among the nine wheat lines examined. Using a doubled haploid (DH) population from an intraspecific
cross between Kitamoe and Münstertaler (KM), we constructed an SSR-based linkage map that consisted of 464 loci: 185 loci
from genomic libraries, 65 loci from the sequence database including ESTs, 213 loci from the SSR markers already reported,
and 1 locus of morphological marker. Although newly developed SSR loci were distributed throughout all chromosomes, clustering
of them around putative centromeric regions was found on several chromosomes. The total length of the KM map spanned 3,441 cM
and corresponded to approximately 86% genome coverage. The KM map comprised of 23 linkage groups because two gaps of over
50 cM distance remained on chromosome 6A. This is a first report of SSR-based linkage map using single intraspecific population
of common wheat. This mapping result suggests that it becomes possible to construct linkage maps with sufficient genome coverage
using only SSR markers without RFLP markers, even in an intraspecific population of common wheat. Moreover, the new SSR markers
will contribute to the enrichment of molecular marker resources in common wheat. 相似文献
14.
Molecular markers and molecular genetic maps are prerequisites for molecular breeding in any plant species. A comprehensive genetic linkage map for cultivated Porphyra haitanensis T. J. Chang et B. F. Zheng has not yet been developed. In this study, 157 double haploid (DH) lines [derived from a YSIII (wildtype) × RTPM (red‐type artificial pigmentation mutant) cross] were used as a mapping population in P. haitanensis. A total of 60 pairs of sequence‐related amplified polymorphism (SRAP) primers and 39 pairs of simple sequence repeat (SSR) primers were used to detect polymorphisms between the two parents. Fifteen SRAP and 16 SSR polymorphic primer pairs were selected to analyze the DH population. A linkage genetic map comprising 67 SRAP markers and 20 SSR markers in five linkage groups, with a total length of 830.6 cM and an average of 10.13 cM between markers, was constructed. The markers were distributed evenly in all linkage groups without clustering. The linkage groups comprised 12–23 markers ranging in length from 134.2 to 197.3 cM. The estimated genome length of P. haitanensis was 942.4 cM, with 88.1% coverage. This is the first report of a comprehensive genetic map in P. haitanensis. The map presented here will provide a basis for the development of high‐density genetic linkage maps and lay the foundation for molecular breeding work in P. haitanensis. 相似文献
15.
Li X Wang X Wei Y Brummer EC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(4):667-679
Segregation distortion (SD) is often observed in plant populations; its presence can affect mapping and breeding applications.
To investigate the prevalence of SD in diploid alfalfa (Medicago sativa L.), we developed two unrelated segregating F1 populations and one F2 population. We genotyped all populations with SSR markers and assessed SD at each locus in each population. The three maps
were syntenic and largely colinear with the Medicago truncatula genome sequence. We found genotypic SD for 24 and 34% of markers in the F1 populations and 68% of markers in the F2 population; distorted markers were identified on every linkage group. The smaller percentage of genotypic SD in the F1 populations could be because they were non-inbred and/or due to non-fully informative markers. For the F2 population, 60 of 90 mapped markers were distorted, and they clustered into eight segregation distortion regions (SDR). Most
SDR identified in the F1 populations were also identified in the F2 population. Genotypic SD was primarily due to zygotic rather than allelic distortion, suggesting zygotic not gametic selection
is the main cause of SD. On the F2 linkage map, distorted markers in all SDR except two showed heterozygote excess. The severe SD in the F2 population likely biased genetic distances among markers and possibly also marker ordering and could affect QTL mapping of
agronomic traits. To reduce the effects of SD and non-fully informative markers, we suggest constructing linkage maps and
conducting QTL mapping in advanced generation populations. 相似文献
16.
Manli Li Nana Yuyama Mariko Hirata Yunwen Wang Jianguo Han Hongwei Cai 《Molecular breeding : new strategies in plant improvement》2010,26(3):467-476
Japanese lawngrass (Zoysia japonica) and Manila grass (Z. matrella) are the two most important and commonly used Zoysia species. A consensus based SSR linkage map was developed for the genus by combining maps from each species. This used previously
constructed maps for two Z. japonica populations and a new map from Z. matrella. The new SSR linkage map for Z. matrella was based on 86 F2 individuals and contained 213 loci and covered a map distance of 1,351.2 cM in 32 linkage groups. Comparison of the three
linkage maps constructed from populations with different genetic backgrounds indicated that most markers exhibited a consensus
order, although some intervals or regions displayed discrepancy in marker orders or positions. The integrated map comprises
507 loci with a mean interval of 4.1 cM, covering a map distance of 2,066.6 cM in 22 linkage groups. The SSR-based map will
allow marker-assisted selection and be useful for the mapping and cloning of economically important genes or quantitative
trait loci. 相似文献
17.
Kei Sato Yohei Mukainari Ken Naito Kenji Fukunaga 《Molecular breeding : new strategies in plant improvement》2013,31(3):675-684
We attempted genetic analysis and mapping of a gene responsible for the trait “spikelet-tipped bristles” (stb) in foxtail millet, Setaria italica (L.) P.Beauv., as the first step in positional cloning of the gene. This trait is important not only in grain yield such as grain number per panicle of this millet but also in the evolutionary development of the “bristle grass” clade including genera Setaria, Pennisetum and Cenchrus in subfamily Panicoideae. First of all, we confirmed that this trait is controlled by a single recessive gene, using two populations of F2 plants; one was a cross combination between two Taiwanese landraces and the other was a combination between a Taiwanese landrace and a Japanese landrace. Using the latter of the two F2 populations, with transposon display (TD) markers and simple sequence repeat (SSR) markers developed previously, we constructed a genetic map with 13 linkage groups and mapped the responsible gene (stb1) on chromosome 2. We also developed novel SSR markers by using foxtail millet genome sequence information, and we finally constructed nine linkage groups corresponding to nine chromosomes with a total length of 1287.5 cM, and mapped stb1 more precisely on chromosome 2. This work suggests that the foxtail millet genome sequences recently published are useful for developing genome-wide SSR markers for constructing linkage maps and mapping genes in this millet. 相似文献
18.
The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.) 总被引:1,自引:0,他引:1
R. K. Varshney D. J. Bertioli M. C. Moretzsohn V. Vadez L. Krishnamurthy R. Aruna S. N. Nigam B. J. Moss K. Seetha K. Ravi G. He S. J. Knapp D. A. Hoisington 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(4):729-739
Molecular markers and genetic linkage maps are pre-requisites for molecular breeding in any crop species. In case of peanut
or groundnut (Arachis hypogaea L.), an amphidiploid (4X) species, not a single genetic map is, however, available based on a mapping population derived
from cultivated genotypes. In order to develop a genetic linkage map for tetraploid cultivated groundnut, a total of 1,145
microsatellite or simple sequence repeat (SSR) markers available in public domain as well as unpublished markers from several
sources were screened on two genotypes, TAG 24 and ICGV 86031 that are parents of a recombinant inbred line mapping population.
As a result, 144 (12.6%) polymorphic markers were identified and these amplified a total of 150 loci. A total of 135 SSR loci
could be mapped into 22 linkage groups (LGs). While six LGs had only two SSR loci, the other LGs contained 3 (LG_AhXV) to
15 (LG_AhVIII) loci. As the mapping population used for developing the genetic map segregates for drought tolerance traits,
phenotyping data obtained for transpiration, transpiration efficiency, specific leaf area and SPAD chlorophyll meter reading
(SCMR) for 2 years were analyzed together with genotyping data. Although, 2–5 QTLs for each trait mentioned above were identified,
the phenotypic variation explained by these QTLs was in the range of 3.5–14.1%. In addition, alignment of two linkage groups
(LGs) (LG_AhIII and LG_AhVI) of the developed genetic map was shown with available genetic maps of AA diploid genome of groundnut
and Lotus and Medicago. The present study reports the construction of the first genetic map for cultivated groundnut and demonstrates its utility
for molecular mapping of QTLs controlling drought tolerance related traits as well as establishing relationships with diploid
AA genome of groundnut and model legume genome species. Therefore, the map should be useful for the community for a variety
of applications.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
19.
An SSR-based linkage map of Capsicum annuum 总被引:1,自引:0,他引:1
Yasuhiro Minamiyama Masato Tsuro Masashi Hirai 《Molecular breeding : new strategies in plant improvement》2006,18(2):157-169
There are five cultivated species of pepper, of which Capsicum annuum is the most widely cultivated as a vegetable or spice and the main experimental material of most pepper breeding programs. However, the number of simple sequence-repeat (SSR) markers known for C. annuum is limited. To develop SSR markers for Capsicum species, we constructed four SSR-enriched libraries from the genomic DNA of C.␣annuum, sequenced 1873 clones, and isolated 626 unique SSR clones. A higher percentage of these SSR markers were taken from dinucleotide motif libraries than from trinucleotide motif libraries. Primer pairs for the 626 SSR clones were synthesized and tested for polymorphisms; 594 amplified products were detected with the expected size. However, only 153 products were polymorphic between the parents of our mapping population. Using 106 highly reproducible pairs from the primer pairs, we constructed a linkage map of C. annuum in an intraspecific doubled haploid population (n=117) that contains nine previously reported SSRs as well as AFLP, CAPS, and RAPD markers and the trait of fruit pungency. The map contains 374 markers, including 106 new SSR markers distributed across all 13 linkage groups, and covers 1042 cM. The polymorphism information content (PIC) of these new SSR markers was calculated using 14 lines of Capsicum species. The average number of alleles per locus was 2.9 and the average PIC value was 0.46, even within C. annuum. The SSR markers developed in this study will be useful for mapping and marker-assisted selection in pepper breeding, and the linkage map provides a reference genetic map for Capsicum species. 相似文献
20.
Construction of a framework map for Pinus koraiensis Sieb. et Zucc. using SRAP, SSR and ISSR markers
Min-Min Chen Fujuan Feng Xin Sui Mai-He Li Dan Zhao Shijie Han 《Trees - Structure and Function》2010,24(4):685-693
Genetic linkage maps are essential for molecular breeding program. The first genetic linkage map of Pinus koraiensis, using an F1 progeny of 94 individuals, was constructed in the present paper. One hundred and twenty-two molecular markers
were mapped onto 11 linkage groups, 1 triple and 8 pairs at the linkage criteria LOD 4.0. Among these markers, there were
96 sequence-related amplified polymorphism (SRAP) markers, 25 simple sequence repeat (SSR) markers, and 1 inter-simple sequence
repeat (ISSR) marker. The consensus map gained covers 857.464 cM Kosambi (K) with an average marker spacing of 7.03 cM K.
The presented map provides a basis and crucial information for future genomic studies of P. koraiensis, in particular for quantitative trait loci (QTL) mapping of economically important breeding target traits. 相似文献