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1.
SAM C. BANKS MAXINE P. PIGGOTT JANE E. WILLIAMSON LUCIANO B. BEHEREGARAY 《Molecular ecology resources》2007,7(2):321-323
We describe the development of 13 variable microsatellites developed to investigate population structure and dispersal in the sea urchin Centrostephanus rodgersii. This species is the dominant grazing herbivore in southeast Australian coastal waters and has the ability to modify benthic community structure. The microsatellites we identified showed a range of allele numbers (4–21) and expected heterozygosity (0.32–0.91) in two sampled populations. Contrary to previous findings in free‐spawning marine invertebrates, genotype proportions in neither population deviated significantly from Hardy–Weinberg expectations. 相似文献
2.
Oliver Manlik Delphine Chabanne Claire Daniel Lars Bejder Simon J. Allen William B. Sherwin 《Marine Mammal Science》2019,35(3):732-759
The forecast for the viability of populations depends upon metapopulation dynamics: the combination of reproduction and mortality within populations, as well as dispersal between populations. This study focuses on an Indo‐Pacific bottlenose dolphin (Tursiops aduncus) population in coastal waters near Bunbury, Western Australia. Demographic modeling of this population suggested that recent reproductive output was not sufficient to offset mortality. Migrants from adjacent populations might make up this deficit, so that Bunbury would act as a “sink,” or net recipient population. We investigated historical dispersal in and out of Bunbury, using microsatellites and mitochondrial DNA of 193 dolphins across five study locations along the southwestern Australian coastline. Our results indicated limited gene flow between Bunbury and adjacent populations. The data also revealed a net‐dispersal from Bunbury to neighboring populations, with microsatellites showing that more than twice as many individuals per generation dispersed out of Bunbury than into Bunbury. Therefore, in historic times, Bunbury appears to have acted as a source population, supporting nearby populations. In combination with the prior finding that Bunbury is currently not producing surplus offspring to support adjacent populations, this potential reversal of source‐sink dynamics may have serious conservation implications for Bunbury and other populations nearby. 相似文献
3.
A set of microsatellites markers were developed for Livistona chinensis var. boninensis, an endemic palm tree of the Bonin Islands. We obtained 123 sequences containing unique microsatellites from an enriched
library. Twelve loci were screened for their feasibility using 32 trees. They showed polymorphisms with two to nine alleles
per locus. No significant deviation from Hardy–Weinberg equilibrium was observed for 11 loci. No genotypic disequilibrium
was detected between any two of the loci. Total exclusionary powers for the first and the second parents were 0.978774 and
0.998987, respectively. These markers will allow us to investigate the gene flow within/among populations of the species. 相似文献
4.
We developed five microsatellites from Ulmus minor using an AFLP protocol followed by an enrichment step, with biotinylated primers containing tandem repeats. Loci were characterized using a total of 30 elms from six Spanish populations. Three microsatellites were also transferred to U. laevis and U. glabra. 相似文献
5.
Five microsatellite markers were developed for the snow crab (Chionoecetes opilio) and polymerase chain reaction conditions and/or primer sequences of three previously developed microsatellites were adapted for fluorescent labelling analysis. All loci were analysed in 449 individuals from 11 sampling sites in the northwest Atlantic. The high degree of polymorphism exhibited by these microsatellites (mean of 34 alleles per locus and mean observed heterozygosity of 0.76) suggests that they will be suitable for spatial and temporal genetic analysis of C. opilio populations. 相似文献
6.
J. N. UNDERWOOD P. B. SOUTER E. R. BALLMENT A. H. LUTZ M. J. H. VAN OPPEN 《Molecular ecology resources》2006,6(1):176-178
Here we report the isolation of 44 microsatellites from the brooding, pocilloporid coral, Seriatopora hystrix, developed from a partial genomic DNA library using a repeat enrichment protocol. A further eight previously published microsatellites were also tested; five of these were developed for S. hystrix, whereas three were isolated from corals of the closely related genus Pocillopora. Out of these, we incorporated nine and 10 primer pairs into two multiplex reactions that reliably amplified polymorphic microsatellites in populations from the west and the east coast of Australia, respectively. Number of alleles ranged from three to 22 per locus. 相似文献
7.
Fifteen populations of Abies nordmanniana, originating from all main parts of its distributional area in the Caucasian region, were genotyped for three chloroplast
microsatellites as well as one mitochondrial marker. The chloroplast microsatellites were highly variable, resulting in a
total of 111 haplotypes in 361 analysed individuals, while the mitochondrial marker showed no variation. Analysis of molecular
variance attributed 2.1% of the variation in the microsatellites to be among populations, and no correlation between geographic
distribution and genetic distances among populations could be observed. A simulation study was conducted to investigate to
what extent the low genetic differentiation among populations could be a result of size homoplasy in the applied microsatellites.
However, the simulations indicated that the low differentiation more likely is caused by high gene flow among populations. 相似文献
8.
P. J. Fisher T. E. Richardson R. C. Gardner 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):969-979
Dinucleotide microsatellites were isolated from Pinus radiata using both a standard genomic library and libraries enriched for microsatellites. Locus-specific primers were designed to
amplify 43 unique microsatellites. Thirty two of these loci had interpretable PCR patterns, 11 of which were polymorphic in
a screen of 19 P. radiata individuals; all 11 polymorphic loci contained at least 17 repeats in the sequenced plasmid. Six of the eleven primer pairs
amplified multiple fragments per individual (3–8), suggesting that these loci were present in multiple copies in the genome.
Genotyping a 48-tree P. radiata production population with seven of the most polymorphic microsatellites revealed an average of 17 bands per locus (the multi-copy
microsatellites were treated as one locus). When tested on known pedigrees, both single and multi-copy microsatellites exhibited
co-dominant inheritance and Mendelian segregation. Two loci had null alleles and one locus had a high frequency of non-parental
alleles, suggesting a high mutation rate. Eight of these microsatellites, including five multi-copy loci, were placed on a
partially constructed P. radiata genetic map. Four of the five multi-copy microsatellites had two or more sets of alleles that mapped to the same locus, and
the fifth mapped to two unlinked loci. All seven tested primer pairs amplified PCR products from other species of hard pine,
three amplified products from soft-pine species, and one amplified bands in other conifers.
Received: 10 November 1997 / Accepted: 5 January 1998 相似文献
9.
Genome‐wide survey of Tribolium castaneum microsatellites and description of 509 polymorphic markers
JEFFERY P. DEMUTH DOUGLAS W. DRURY MORGAN L. PETERS J. DAVID VAN DYKEN NICHOLAS K. PRIEST MICHAEL J. WADE 《Molecular ecology resources》2007,7(6):1189-1195
We survey the Tribolium castaneum whole genome sequence for 1–6 bp microsatellite motifs. Of the > 12 000 microsatellites discovered, we screened 981 primer pairs which yielded 509 polymorphic markers. We report polymorphism data for pooled DNA samples from three to 11 geographically widespread T. castaneum populations as well as heterozygosity for a subset of the markers. The composition and chromosomal distribution of microsatellites is briefly discussed in relation to previous studies. 相似文献
10.
S. C. BANKS M. P. PIGGOTT D. A. RAFTOS L. B. BEHEREGARAY 《Molecular ecology resources》2006,6(3):856-858
The Sydney rock oyster (Saccostrea glomerata) is a commercially important bivalve in southeastern Australian. We describe the isolation and characterization of nine microsatellite markers for S. glomerata. The loci are highly polymorphic, with between five and 20 alleles identified among 30 individuals. Expected heterozygosity levels ranged from 0.608 to 0.936. The markers will be used to study natural dispersal, translocations and population structure. We will also use the microsatellites to test the genetic effects of QX disease on oyster populations. This infectious parasitic disease has decimated S. glomerata productivity in a number of areas over the past few decades. 相似文献
11.
M. A. GUEVARA D. CHAGNÉ M. H. ALMEIDA M. BYRNE C. COLLADA J. M. FAVRE L. HARVENGT S. JEANDROZ C. ORAZIO C. PLOMION A. RAMBOER M. ROCHETA F. SEBASTIANI A. SOTO G. G. VENDRAMIN M. T. CERVERA 《Molecular ecology resources》2005,5(1):57-59
We developed 20 polymorphic nuclear microsatellites from Pinus pinaster using genomic libraries enriched with repeated motifs. A total of 25 individuals from five different populations were used to estimate genetic diversity parameters. 相似文献
12.
Alexandra Pavlova Faith M. Walker Rodney van der Ree Silvana Cesarini Andrea C. Taylor 《Conservation Genetics》2010,11(6):2393-2407
The squirrel glider Petaurus norfolcensis occurs across a broad Australian latitudinal range that includes gaps in distribution and potential biogeographic barriers,
creating the potential for evolution of distinct entities within this species. Because of the species’ threatened status in
the southern part of its range, we tested for the presence of geographically based independent evolutionary units among gliders
sampled from southern, and northern coastal populations, using sequences of mitochondrial cytochrome b DNA (mtDNA) and a set of five nuclear microsatellites in 258 individuals. Our analyses suggest that an initial northward
colonisation in the early- to mid-Pleistocene was followed by isolation by distance and, eventually, divergence between the
sampled coastal and southern populations in the mid- to late-Pleistocene. We propose that the previously large and diverse
southern populations have declined coincidentally with the replacement of wet forests by open sclerophyll woodlands during
the preceding few million years. By contrast coastal populations further north appear to have been expanding and at present
have an effective population size several times greater than southern populations. These results suggest that the two forms
are on different evolutionary trajectories and should be treated separately for conservation purposes. It is highly desirable
that loss of southern populations be prevented to maintain the unique genetic diversity accumulated over a considerable evolutionary
timescale. 相似文献
13.
Nina Yasuda Satoshi Nagai Chunlian Lian Masami Hamaguchi Takeshi Hayashibara Kazuo Nadaoka 《Conservation Genetics》2008,9(4):1011-1013
We isolated 11 polymorphic microsatellites from blue coral (Heliopora coerulea), whose conservation and management are of great concern. The number of alleles ranged from 3 to 20 with an average of 5.5,
and the observed and expected heterozygosities ranged from 0.115 to 0.833 and from 0.371 to 0.915, respectively. These loci
are useful for conservation genetics in H. coerulea populations. 相似文献
14.
The European roe deer (Capreolus capreolus) is an interesting model for molecular ecology studies because of its abundance and adaptability across a range of environments (including human‐modified habitats), and because of its increasing impacts on agricultural crops and on regenerating forests. We identify polymorphic microsatellites in two managed populations of roe deer in France by using cross‐species amplification of primers from other Cervids and from Bovids. Of the 62 primer pairs tested, 45 amplified microsatellites in roe deer, and 34 were polymorphic. Eleven primer pairs were selected for multiplex gel‐loading for routine genotyping of the studied populations. 相似文献
15.
MA. CARMEN A. ABLAN 《Molecular ecology resources》2006,6(1):170-172
Ten polymorphic microsatellites were isolated from the red‐bellied yellow tail fusilier Caesio cuning, a reef‐associated fish which occurs widely in the Indo‐Pacific region. The species is exploited by both small‐scale and commercial fisheries. Fifty individuals from six populations were genotyped using primers that reliably amplified 10 polymorphic loci. The number of alleles per locus ranged from three to 11. Observed heterozygosity (HO) and expected heterozygosity (HE) ranged between 0.400 to 0.880 and 0.337 to 0.843, respectively. These microsatellite loci may be used to study population structure, genetic diversity and connectivity of C. cuning in the range of its distribution. 相似文献
16.
N. M. ENDERSBY S. W. MCKECHNIE H. VOGEL L. J. GAHAN S. W. BAXTER P. M. RIDLAND A. R. WEEKS 《Molecular ecology resources》2005,5(1):51-53
Diamondback moth, Plutella xylostella (L.), is a worldwide agricultural pest that has developed resistance to many insecticides used for its control. Population structure and gene flow are yet to be determined for P. xylostella in Australia, but are important factors for the design of effective control strategies. We have isolated six polymorphic microsatellite markers: three from a partial genomic library, two from an Expressed Sequence Tagged library and one from an aminopeptidase intron of P. xylostella. These microsatellites will be used to determine population structure and gene flow in Australian populations of P. xylostella to improve insecticide resistance management. 相似文献
17.
Matsuoka Y Mitchell SE Kresovich S Goodman M Doebley J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):436-450
To evaluate the performance of microsatellites or simple sequence repeats (SSRs) for evolutionary studies in Zea, 46 microsatellite loci originally derived from maize were applied to diverse arrays of populations that represent all the
diploid species of Zea and 101 maize inbreds. Although null phenotypes and amplification of more than two alleles per plant were observed at modest
rates, no practical obstacle was encountered for applying maize microsatellites to other Zea species. Sequencing of microsatellite alleles revealed complex patterns of mutation including frequent indels in the regions
flanking microsatellite repeats. In one case, all variation at a microsatellite locus came from indels in the flanking region
rather than in the repeat motif. Maize microsatellites show great variability within populations and provide a reliable means
to measure intraspecific variation. Phylogeographic relationships of Zea populations were successfully reconstructed with good resolution using a genetic distance based on the infinite allele model,
indicating that microsatellite loci are useful in evolutionary studies in Zea. Microsatellite loci show a principal division between tropical and temperate inbred lines, and group inbreds within these
two broad germplasm groups in a manner that is largely consistent with their known pedigrees.
Received: 10 February 2001 / Accepted: 21 May 2001 相似文献
18.
MARK A. RENSHAW JOHN C. PATTON CAIRD E. REXROAD JOHN R. GOLD 《Molecular ecology resources》2006,6(4):1162-1164
Eighteen nuclear‐encoded microsatellites from a genomic DNA library of greater amberjack, Seriola dumerili, were isolated and characterized. The microsatellites include 13 perfect (five tetranucleotide and eight trinucleotide) and five imperfect (three tetranucleotide, one trinucleotide and one combination dinucleotide/trinucleotide) repeat motifs. The number of alleles at the 18 microsatellites among a sample of 29 fish ranged from two to 20; gene diversity (expected heterozygosity) ranged from 0.068 to 0.950, whereas observed heterozygosity ranged from 0.069 to 0.966. Following Bonferroni correction, genotypes at all 18 microsatellites fit expectations of Hardy–Weinberg equilibrium, and all pairwise comparisons of microsatellites did not deviate significantly from genotypic equilibrium. Greater amberjack support commercial and recreational fisheries along both the Atlantic and the Gulf coasts of the USA and represent a species with potential for worldwide aquaculture. The microsatellites developed will be useful for population genetic studies of ‘wild’ populations and breeding studies of domesticated populations. 相似文献
19.
Genetic diversity of common carp from two largest Chinese lakes and the Yangtze River revealed by microsatellite markers 总被引:9,自引:1,他引:8
Six polymorphic microsatellites (eight loci) were used to study the genetic diversity and population structure of common carp
from Dongting Lake (DTC), Poyang Lake (PYC), and the Yangtze River (YZC) in China. The gene diversity was high among populations
with values close to 1. The number of alleles per locus ranged from 2 to 11, and the average number of alleles among 3 populations
ranged from 6.5 to 7.9. The mean observed (H
O) and expected (H
E) heterozygosity ranged from 0.4888 to 0.5162 and from 0.7679 to 0.7708, respectively. Significant deviations from Hardy–Weinberg
Equilibrium expectation were found at majority of the loci and in all three populations in which heterozygote deficits were
apparent. The analysis of molecular variance (AMOVA) indicated that the percent of variance among populations and within populations
were 3.03 and 96.97, respectively. The Fst values between populations indicated that there were significant genetic differentiations for the common carp populations
from the Yangtze River and two largest Chinese freshwater lakes. The factors that may result in genetic divergence and significant
reduction of the observed heterozygosity were discussed. 相似文献
20.
Y. NACIRI‐GRAVEN S. CAETANO D. PRADO R. T. PENNINGTON R. SPICHIGER 《Molecular ecology resources》2005,5(3):542-545
Eleven dinucleotide microsatellites were developed in Geoffroea spinosa (Leguminosae), a widespread tree of the seasonally dry Neotropical forests, and characterized on six populations from Peru, Argentina and Paraguay. Four of them amplified on the Peruvian populations only, probably because of mutations in the microsatellite flanking regions in the other populations. Ten microsatellites were found polymorphic, with within population gene diversities ranging from 0.17 to 0.95, and a number of alleles varying from seven to 19. A significant overall genetic differentiation was also found (θ = 0.212; P < 0.01). 相似文献