矩形车轮虫的再描述

目的:我们对泸州市鲇类寄生车轮虫进行调查,对于已经发现,但由于原来的技术水限制需要重新描述的,或缺少资料的车轮虫,给予重新描述或补充新的资料。方法:用国际间统一的干银法染色以显示车轮虫的附着盘结构和口围绕度,用Lom(1958)倡导的"统一的特定方法"(Uniform specifi characteristic system)进行测量,用Van&Bassion(1989)提供的方法描述车轮虫的齿体。结果:对寄生在泸州地区养殖鱼类斑点叉尾鮰(Ietalurus punetaus)的鳃上的车轮虫进行了描述,该车轮虫是矩形车轮虫(Trichodina rectangli)。结论:矩形车轮虫是陈启鎏和谢杏人1964年在青鱼、草鱼等鱼身体上发现的,但是没有显微照片。国际上有许多它的同种异名的车轮虫的描述,这些文献也只有附着盘的显微照片。斑点叉尾鮰是矩形车轮虫的新寄主记录。本研究用国际统一的方法对矩形车轮虫进行描述,并绘出了矩形车轮虫的齿体定位图,提供了完整的统计数据,以及完整的显微照片,即附着盘、口围绕度和细胞核显微照片。     

瓦氏黄颡鱼2种寄生车轮虫形态分类

采用国际上使用的"统一特定描述"和"齿体定位描述"方法对寄生在瓦氏黄颡鱼(Peleteobagrusvachelli)鳃上的适度车轮虫(Trichodina modesta)和马氏车轮虫(T.mastu)2种车轮虫的虫体外形、附着盘、齿体、辐线、中央颗粒、核器、口围绕体等形态特征进行了较为详细的描述,并分别与已报道的其他适度车轮虫、马氏车轮虫种群及相似种的形态特征进行了比较。实验证实了瓦氏黄颡鱼为适度车轮虫的寄主新纪录。     

寄生于重庆地区鲢、鳙及草鱼五种外寄生车轮虫的描述

对采自重庆地区寄生于鲢、鳙及草鱼鳃上的5种外寄生车轮虫进行了形态学及分类学研究,其分别隶属于：车轮虫属、小车轮虫属及三分虫属,其中包含1新种和中国2新纪录。即钝圆车轮虫Trichodina obtusiformis sp．nov．,急尖车轮虫T．acuta Lom,1961,显著车轮虫T．nobilis Chen,1963,周丛小车轮虫Trichodinella epizootica（Rabbe,1950）Sramek-Husek,1953和大型三分虫Tripartiella macrosoma Basson ＆ Van As,1987。文中对新种进行了详细的描述并提供了新种及已知种的附着盘银染标本之显微照片及齿体线条图。     

鲫寄生车轮虫一新种的描述

本文记述了寄生在鲫鱼鳃上的车轮虫属一新种——Trichodina paranigra sp．nov．。文中采用Lom(1960)所倡导的统一特定方法及由Van As ＆ Basson(1989)提出的齿体各鉴定特征的定位描述,对本新种的各形态学特征(包括虫体外形、附着盘、齿体、辐线、中央颗粒、核器、口围绕体等)进行了较为详尽的描述,并对本种与相似种进行了比较研究。文中还为齿体定位描述模式图新增了两个参数。     

重寄生车轮虫的新分布和再描述

于乌鳢（Chanaa argas）鳃上获得一外寄生原动物,经鉴定为一缘毛类目纤毛虫——重寄生车轮虫（Trchodina hypervatositis Chen ＆ Hsish,1984）。按照国际上对车轮虫形态学特征鉴定的标准,本工作用齿体定位描述对该虫进行了研究,补充并完善了重寄生车轮虫的形态描述,如缘膜宽、大核内外径、齿长及齿棘长等数据,同时提供了显微照片（图1）及齿体定位描述图（图2）。     

云南地区水生经济动物外寄生车轮虫的研究

在对云南地区淡水车轮虫的调查研究过程中,在几大重要水生经济动物,即泥鳅、鲫、鲤、鳙、斑点叉尾鮰及牛蛙蝌蚪上获得8种外寄生车轮虫。其中车轮虫属4种,即虱性车轮虫Trichodina pediculus、杜氏车轮虫T.domerguei、拟黑色车轮虫T.paranigra和适度车轮虫T.modesta;拟车轮虫属1种,即圆形拟车轮虫Paratrichodina rotundiformis;小车轮虫属3种,即周丛小车轮虫Trichodinella epizootica、卡普小车轮虫T.carpi和纤细小车轮虫T.subtilis。采用活体观察、甲基绿-派洛宁染色及干银法,并运用国际统一的特定方法及齿体定位描述法对该8种车轮虫进行了详细的形态学研究。     

重庆地区泥鳅体外寄生车轮虫的形态分类学研究

在重庆地区鱼类寄生虫的调查过程中,从淡水鱼泥鳅的鳃上获得四种外寄生车轮虫,经鉴定隶属车轮虫科（Trichodinidae）、车轮虫属（Trichodina）,含1车轮虫未定种Trichodina．sp．和3已知种,即：适度车轮虫（TrichodinamodestaLom,1970）,斜齿车轮虫（Trichodina lechriodentata Zhao＆Tang,2007）及拟黑色车轮虫（TrichodinaparanigraTangetal,2005）。车轮虫的形态学特征统计采用Lom（1958）倡导的统一特定方法,齿体的定位描述则采用Van As＆Basson（1989）和Tang,Zhao＆Chen（2005）改进的方法进行     

重庆地区鲫鱼外寄生车轮虫的记述及一新种描述

报道了寄生于重庆地区淡水养殖鲫鱼鳃上的车轮虫属2种,小车轮虫属1种,其中1新种和中国2新纪录种.文中对新种作了详细的描述并提供了新种(包括成体与幼体分裂期)及新纪录种附着盘的银染标本照片及齿体的线条图.     

广西地区罗非鱼外寄生车轮虫的形态分类学及多样性研究

采用活体观察、吉姆萨与干银法染色等技术,运用国际间统一特定方法及齿体定位描述法对采自广西地区的罗非鱼(单性鱼吉富、奥尼系列)的外寄生车轮虫进行详细的形态分类学与多样性研究,研究结果共计获得车轮虫属2种,拟车轮虫属与三分虫属各1种,分别为:刺纹车轮虫Trichodina centrostrigata Basson et al.,1983、异齿车轮虫Trichodina heterodentata Duncan,1977、非洲拟车轮虫Paratrichodina africana Kazubski&El-Tantawy,1986与直齿三分虫Tripartiella orthodens Basson&Van As,1987。其中,非洲拟车轮虫为中国新纪录,刺纹车轮虫和直齿三分虫为中国广西地区新纪录。非洲拟车轮虫表现出寄主和寄生部位的专一性;进一步应用SPSS统计软件对非洲拟车轮虫进行了种群差异性分析。     

基于附着盘特征量化的眉溪小车轮虫种内研究

研究结合形态学与统计学研究方法,对外寄生于三种不同宿主(鲢、鳙与麦穗鱼)的眉溪小车轮虫Trichodinella myakkae(Mueller, 1937)?rámek-Hu?ek, 1953进行了附着盘特征量化的种内比较研究。研究结果显示:眉溪小车轮虫三种群(鲢种群、鳙种群与麦穗鱼种群)在虫体直径方面的P值为0.136(P>0.05),在附着盘直径、齿环直径和齿体纵长三方面的P值分别为0.009、0.000与0.000(P<0.01);三种群在齿环直径/虫体直径方面的P值为0.000与0.004(P<0.01)。相关性研究结果显示:虫体直径、附着盘直径、齿环直径与齿体纵长两两间均呈显著正相关(P<0.01),缘膜宽与附着盘中其他结构不存在显著相关性(P>0.05)。上述研究结果表明,除虫体直径外,三种群在附着盘直径、齿环直径、齿体纵长,以及齿环直径/虫体直径方面均存在显著差异,其中鲢种群是最大的种群;相关性研究表明,虫体直径主要受附着盘直径的影响,反之亦然;齿环直径主要受齿体纵长的影响,反之亦然;齿体数主要受附着盘大小所影响;缘膜宽则不受附着盘相关结构...     

Trichodinid ectoparasites (Ciliophora: Peritrichida) from the gills of cultured marine fishes in China,with the description of Trichodinella lomi n. sp.

Three species of cultured percoids, each heavily infested by trichodinid ectoparasites, were examined from over-wintering pools in Qingdao, China during the winter of 1995. A total of five species of trichodinids belonging to three genera, Trichodinella Sramek-Husek, 1953, Dipartiella Shtein, 1961 and Trichodina Ehrenberg, 1838 were recorded. One new species, Trichodinella lomi n. sp., is described, the denticle of which is characterised by its acutely triangular blade, comma-like central part and inconspicuous ray. The morphology of Trichodina murmanica Poljansky, 1955 was re-investigated. Three other little-known species are redescribed: Dipartiella simplex (Raabe, 1959) Shtein, 1961, Trichodina japonica Imai, Miyazaki & Nomura, 1991 and T. domerguei (Wallengren, 1897).     

鲫鱼寄生车轮虫一新种的研究

本文记述了寄生在鲫鱼(Carassius auratus)体表和鳃上一种新的车轮虫(Trichodina carassii sp.nov.),虫体直径70(54.6—80.4)μm,附着盘直径58(51.6—67.2)μm,齿环直径32(27.0—36.2)μm,齿体25—30个,齿环中央有7—14个旧齿体残余物形成的颗粒结构。大核马蹄形。小核短杆状,在大核一端的外侧,其位置略有变动。口沟390—400°。齿钩镰刀状,齿棘较直,侧面具有若干个缺刻,末端钝圆或平截,近锥体的后方有一个不太明显的小突起。       

Two trichodinid ectoparasites from marine molluscs in the Yellow Sea,off China,with the description of <Emphasis Type="Italic">Trichodina caecellae</Emphasis> n. sp. (Protozoa: Ciliophora: Peritrichia)

The morphology and infraciliature of two ectoparasitic ciliates, Trichodina caecellae n. sp. and T. ruditapicis Xu, Song & Warren, 2000, parasitising the gills of marine molluscs from the Shandong coast of the Yellow Sea, China, were investigated following wet silver nitrate and protargol impregnation. T. caecellae was found on the small marine sand clam Caecella chinensis Deshayes and is distinguished mainly by the acute triangle-like blade, the very delicate central part and the needle-shaped ray. T. ruditapicis was studied based on four populations from three clams: two populations from Ruditapes philippinarum (Adams) and one each from Saxidomus purpuratus (Sowerby) and Solen grandis Dunker. All four populations fell within the range of morphometry and agreed closely in the overall appearance of the adhesive disc. However, variability was found in the denticle structure, especially in populations from different host clams. Our observations suggest that denticle morphology may be more or less variable between and within populations, and that such minor differences should not be overestimated. It should be emphasised that, except for the denticle morphology, the bright granules or circles in the centre of the adhesive disc represent another important feature facilitating the identification of this trichodinid species.     

A new species of <Emphasis Type="Italic">Trichodina</Emphasis> Ehrenberg, 1830 (Ciliophora: Trichodinidae) infecting farmed <Emphasis Type="Italic">Clarias gariepinus</Emphasis> (Burchell) (Siluriformes: Clariidae) in Cuba

A new species of Trichodina Ehrenberg, 1830 collected from the skin and fins of farmed North African catfish Clarias gariepinus (Burchell) fingerlings, is described. The new species can be distinguished from other trichodinids by the characteristics of the adhesive disc, especially by the great number of denticles. Trichodina merciae n. sp. is morphologically similar to T. renicola (Mueller, 1931) and T. marplatensis Martorelli, Marcotegui & Alda, 2008, in the number of denticles, but differs in the morphometric data, denticle morphology, environment and location. Trichodina merciae n. sp. has broad sickle-shaped blades and thin, straight rays, while T. marplatensis has broad club-shaped blades and wide S-shaped rays. Besides, denticle length, blade length, ray length, width of central part and denticle span of the new species are greater than T. marplatensis. However, the diameter of denticle ring and the diameter of the central area in T. marplatensis is larger than the ones in T. merciae n. sp. This is the first record of freshwater ectoparasite trichodinid with an average number of denticles greater than 50.     

Observations on trichodinid ectoparasites (Ciliophora: Peritricha) from the gills of maricultured molluscs in China, with descriptions of three new species of Trichodina Ehrenberg, 1838

During surveys of the trichodinid parasites in mariculture beds off the coast of Shandong Province, China, four species of the genus Trichodina from the gills of marine molluscs were investigated and morphologically studied. Of these, three are described as new: T. ruditapicis n. sp. from Ruditapes philippinarum (Veneridae), T. scapharcae n. sp. from Scapharca subcrenata (Arcidae) and T. mactrae n. sp. from Mactra veneriformis (Mactridae). One little-known species, T. macomarum Raabe & Raabe, 1959, is redescribed from M. veneriformis. Taxonomic and morphometric data for these trichodinids based on wet silver nitrate and protargol-impregnated specimens are presented. For each of the new species, comparisons with closely related species are provided.