NaCl 胁迫对甘草生长及抗氧化酶活性的影响

目的:通过分析不同浓度NaCl胁迫下甘草生长和抗氧化酶活性的变化探讨甘草对盐分胁迫的适应性机理;方法:采用不同浓度的NaCl溶液(配成Hoagland液)处理盆栽一年生甘草,分别于35d、70d和105d取样,测定甘草株高、地上部分鲜、干重、根鲜、干重及甘草叶片SOD、POD、CAT活性,分析各生长指标与抗氧化酶活性的相关性;结果:NaCl胁迫70d和105d,0.6%和0.9%处理组的株高、地上部分鲜、干重及根鲜、干重均显著低于CK,SOD、POD及CAT活性均显著高于CK,经相关性分析得知,SOD、POD及CAT活性与各生长指标均负相关,其中POD活性与各生长指标极显著负相关;结论:甘草对NaCl胁迫的响应有胁迫时间和浓度的依赖性,在遭遇胁迫时,通过改变自身的生长和提高抗氧化酶活性,来提高机体的抗盐能力。     

硒对水稻的生态毒理效应及临界指标研究

通过土壤添加硒盆栽试验,研究了黄棕壤不同浓度Se对水稻生物量、叶绿素含量、抗氧化酶系统的影响.结果表明,低浓度Se(<8mg·kg^-1)对水稻根和地上生物量、叶绿素a含量、叶绿素a/b有促进作用,并整体提高了作物抗氧化酶系统,使MDA下降,SOD、CAT、POD、GSHPx活性相应提高.而高浓度Se(>16mg·kg^-1)对水稻根和地上生物量、叶绿素a含量、叶绿素a/b有明显的抑制作用,对抗氧化酶系统产生胁迫效应,使GSHPx明显上升,MDA上升,SOD、CAT、POD酶活性明显下降.各项生理指标与土壤Se处理浓度之间相关分析表明,土壤Se浓度与地上部干重相对百分率、根干重百分率、叶绿素a/b、MDA、SOD、CAT、POD之间有显著相关性.比较胁迫效应10%值的临界指标EC10值表明,作物地上部干重抑制率作为土壤临界值制定的依据更为可靠,EC10为21mg·kg^-1.     

锗对水稻的生态毒理效应及临界指标

土壤添加 Ge盆栽试验研究表明 ,低浓度 Ge(<4 m g/ kg)对水稻根和地上生物量、叶绿素 a含量有促进作用 ,并整体提高了作物抗氧化酶能力 ,使 MDA下降 ,SOD、CAT、POD、GSH- Px活性相应提高。而高浓度 Ge(>15 mg/ kg)对水稻根和地上生物量、叶绿素 a含量有明显的抑制作用 ,对抗氧化酶系统产生胁迫效应 ,使 POD明显上升 ,MDA上升 ,SOD、CAT酶活性明显下降。在高浓度 Ge胁迫下 ,植物细胞中抗氧化作用酶系统的受损打破了植物细胞内活性氧产生与清除间的正常平衡状态 ,积累过量的活性氧 ,使活性氧产生并积累 ,过量的活性氧引起膜脂过氧化程度加重 ,从而对植物产生毒害。各项生理指标与土壤 Ge处理浓度之间相关分析表明 ,土壤 Ge浓度与地上部干重相对百分率 ,根干重百分率、叶绿素 a/ b、MDA、SOD、CAT之间有显著相关性。比较胁迫效应 10 %值的临界指标 EC1 0 值表明 ,作物地上部干重抑制率和根干重抑制率作为土壤临界值制定的依据更为可靠 ,EC1 0 为 7m g/ kg     

低温胁迫下玉米幼苗根系受外源海藻糖调控的生理表现

为了探究海藻糖对增强玉米幼苗抗低温能力的生理表现,以玉米"先玉335"为试验材料,采用盆栽试验,分析12 mmol·L-1外源海藻糖对低温胁迫不同时间(0、2、4、6和8d)下玉米幼苗根系生物量、抗氧化酶活性及渗透调节物质的影响。结果表明:低温胁迫显著抑制玉米幼苗根系生长及干物质累积,诱导抗氧化酶活性增加,破坏细胞渗透调节水平;低温胁迫后应用外源海藻糖,玉米幼苗根系根表面积、根长及鲜干重显著增加,根系内SOD、POD和CAT活性呈现先升高后降低趋势,MDA含量及相对电导率在玉米幼苗根系内显著降低,脯氨酸、可溶性蛋白、可溶性糖含量也呈现先升高后降低趋势;在低温胁迫第6天时,T+L处理的根系根表面积、根长及鲜干重分别较CK+L增加了18.25%、4.73%、2.48%和21.43%;T+L处理后根系内SOD、POD和CAT活性分别较CK+L处理提升17.26%、23.79%和30.78%;T+L处理后根系内MDA含量和相对电导率值分别较CK+L处理降低45.13%和63.25%;T+L处理后的脯氨酸、可溶性蛋白和可溶性糖含量在低温胁迫第6天时最高,与CK+L处理相比差异显著,且分别较CK+L高出2.93、0.51和2.58倍。可见,海藻糖能够调控低温胁迫下玉米幼苗根系生理水平,提高玉米幼苗抗逆能力,促进玉米根系生长及生物量增加,海藻糖对作物生长的生理调控能力与低温存在协同作用,在低温环境下,海藻糖对作物体内抗氧化系统酶活表达及渗透物质的积累有显著提升效果。     

腐植酸浸种对盐碱胁迫下小麦幼苗抗氧化系统的影响

对龙麦26和克旱16两种不同基因型春小麦品种进行腐植酸浸种处理,研究了腐植酸对盐(NaCl)、碱(Na CO3)两种胁迫下小麦幼苗抗氧化系统的影响.结果表明:盐碱胁迫下小麦幼苗叶片脯氨酸含量增加,膜透性增强,地上部鲜质量降低;盐胁迫下,过氧化氢酶(CAT)活性升高,过氧化物酶(POD)活性降低,超氧化物歧化酶(SOD)活性先升后降,而碱胁迫下CAT和POD活性均升高,SOD活性降低;碱胁迫处理的小麦幼苗叶片抗氧化酶系统活性高于同浓度的盐胁迫处理;腐植酸浸种提高了小麦叶片谷胱甘肽含量,提高了SOD和CAT活性,有效缓解了盐碱胁迫对幼苗生长的影响.不同基因型小麦品种在抵御盐碱胁迫的能力和机制上存在一定差异,且与盐离子组成、浓度间存在一定互作,并受浸种方式调控.     

土壤NaCl含量对榉树幼苗生理特性的影响

在温室盆栽条件下,用0～0.60% NaCl(占土壤干重)处理1年生榉树幼苗后7～35 d对其形态以及叶片渗透性调节物、抗氧化酶活性等5项生理指标的变化进行观察分析,以明确榉树在不同含量盐胁迫条件下的生理适应性.结果显示:(1)不同浓度NaCl处理对榉树幼苗存活和形态有不同的影响,低盐(0.15%)处理,植株生长正常;在0.30%、0.45%和0.60% NaCl处理下,随NaCl含量的增加和胁迫时间的延长,苗木相继出现叶缘焦枯、萎蔫直至死亡等现象,至胁迫35 d时,植株存活率分别为60%、10%和0;(2)在0.15% NaCl处理下其叶片相对电导率和脯氨酸、MDA含量、以及SOD、POD活性等生理指标与对照无显著差异,而在0.30%～0.60%处理下,叶片相对电导率、脯氨酸和MDA含量均显著升高,而SOD和POD活性则呈先升后降的趋势,且随处理时间的增加均与对照差异显著(P<0.05)或极显著(P<0.01);(3)各处理POD活性峰值出现时间晚于SOD活性的峰值,如第7天后,0.45%、0.60%处理的SOD活性均呈快速下降趋势,而POD的活性则处于逐渐上升之中,表明SOD对NaCl处理反应迅速,POD在高盐处理初期(15 d)可保持较高的水平,有助于降低膜质过氧化对细胞膜系统的损伤.研究表明,榉树幼苗能够在0.15% NaCl的土壤中正常生长,而在0.30% NaCl土壤中生长35 d时幼苗的死亡率达40%,即榉树幼苗耐受NaCl胁迫的临界浓度大约在0.15%～0.30%之间.     

NaCl胁迫对'金光'杏梅幼苗生长及其生理指标的影响

以‘金光’杏梅盆栽幼苗为试验材料,研究了NaCl胁迫对‘金光’杏梅幼苗生长和生理指标的影响.结果表明:NaCl胁迫显著抑制了‘金光’杏梅幼苗的生长,随着NaCl浓度的升高,‘金光’杏梅的株高、叶面积、叶鲜重、叶干重、茎干重、根干重、叶绿素含量和净光合速率均呈下降趋势;叶片细胞膜透性、丙二醛(MDA)、可溶性糖、脯氨酸(Pro)含量以及POD活性显著增加;而SOD活性在低NaCl浓度下上升,在高NaCl浓度下下降.     

硫酸铈对硝酸钙胁迫下番茄叶片抗氧化酶活性及光合性能的影响

以番茄品种‘合作908’幼苗为材料,研究了80 mmol/L硝酸钙胁迫下叶面喷施10 mg/L硫酸铈对番茄幼苗生长、叶片抗氧化酶活性及光合性能的缓解效应。结果显示：硝酸钙胁迫导致番茄幼苗的株高、茎粗、鲜重、地上和地下部干重显著降低,叶片SOD、POD、CAT活性减弱,净光合速率、气孔导度、蒸腾速率以及胞间CO₂浓度均显著下降,而MDA含量和质膜透性显著升高;与硝酸钙胁迫处理相比,叶面喷施硫酸铈处理对硝酸钙胁迫下的番茄幼苗株高、茎粗、鲜重和干重等营养生长具有不同程度的促进作用,同时显著提高叶片SOD、POD和CAT活性,有效降低叶片MDA含量和细胞膜透性,并且显著提高了叶片净光合速率、气孔导度、蒸腾速率。研究表明,叶面喷施硫酸铈可诱导增强硝酸钙胁迫下番茄幼苗叶片的抗氧化酶活性,有效降低膜脂过氧化程度,维持较高水平的光合性能,从而一定程度上缓解硝酸钙胁迫对番茄幼苗生长造成的伤害,增强其耐受盐胁迫的能力。     

番茄高温高湿胁迫后的补偿生长

为了解高温高湿复合胁迫对番茄的影响,以番茄品种"金冠五号"(Jinguan 5)为对象,设计温度、相对湿度和处理天数三因素正交试验,温度设置4个水平(昼温/夜温),即32℃/22℃、35℃/25℃、38℃/28℃、41℃/31℃,相对湿度设置3个水平,为50%±5%、70%±5%、90%±5%,持续时间设为4个水平,为3、6、9、12 d。以28/18℃、50%～55%环境下处理的番茄幼苗为对照组(CK)。分别在高温高湿胁迫后及恢复生长的7、14、21、28 d进行取样,测定生长指标、抗氧化酶活性及叶绿素含量。结果表明:高温胁迫后,35、38、41℃处理下番茄干重显著降低,叶面积显著增加,41℃番茄干重较CK降低12.89%,高温处理后叶绿素a、叶绿素b、类胡萝卜素含量低于CK,SOD、POD、CAT酶活性均高于CK,且随温度升高,酶活性呈先上升后下降的趋势,38℃处理SOD、POD、CAT酶活性最高,分别较CK增加121.14%、35.41%、55.13%;从湿度来看,相同高温条件下,RH70%处理番茄的生长最好,RH70%处理番茄SOD、POD、CAT酶活性、叶绿素含量显著高于RH50%和RH90%湿度处理;适当的高温能够促进番茄的生长,增加抗氧化酶活性,32℃以下番茄生长呈超补偿;38℃及其以上番茄生长严重受阻;高温条件下,增加湿度能够一定程度上缓解高温对植株的胁迫,但这种作用也是有限的,并不能够完全抵消高温胁迫的伤害。     

AMF对弱光及盐胁迫下甜瓜生长和抗氧化酶活性的影响

以甜瓜(Cucumis melo L.)品种"中蜜3号"为试材,摩西球囊霉菌(Glmous mosseae,GM)为供试菌种,采用温室盆栽试验研究接种丛枝菌根真菌(AMF)对弱光及盐胁迫下甜瓜生长和抗氧化酶活性的影响,以明确AMF对甜瓜复合逆境下的增抗作用并探讨其生理机制。结果显示:(1)在弱光及盐胁迫条件下,甜瓜幼苗生长受到明显抑制,其株高、干重和鲜重均明显降低,同时体内可溶性糖、可溶性蛋白、脯氨酸和MDA含量以及SOD、POD、CAT活性均比对照显著升高。(2)弱光及盐胁迫下,接种AMF可显著促进甜瓜幼苗的生长,菌根侵染率随胁迫时间延长与盐浓度呈负相关关系。(3)弱光及盐胁迫下,接种AMF进一步提高了甜瓜幼苗体内可溶性糖、可溶性蛋白、淀粉和脯氨酸含量及SOD、POD、CAT活性,显著降低了MDA含量,且叶片SOD、POD活性变化大于根系,CAT则相反。研究表明,AMF可通过促进弱光及盐胁迫下甜瓜生长和抗氧化酶活性的提高,有效降低体内膜脂过氧化水平,从而增强植株对弱光及盐胁迫的耐性。     

钼营养对甘草生理和生长特性的影响

目的：探讨不同浓度的钼营养水平对甘草生长和生理特性的影响。方法：以一年生的甘草移栽苗为试验材料,采用盆栽蛭石的试验方法,共设置4个钼浓度水平,分别为0mg·L-1,0.52mg·L-1,5.2mg·L-1和10.4mg·L-1,其中0.52mg·L-1即正常Hoagland营养液中钼的浓度。每周向盆内浇灌营养液,以达到处理的目的。采用LI-6400光合仪测定其光合生理指标以及植物生理学常规方法进行甘草叶片色素和抗氧化酶活性的测定。采用电子天平分别测定不同处理下的甘草地上、根的鲜重和干重等。结果：结果表明,甘草的各项生理和生长指标随着钼处理浓度的增加而增加,显著增加了甘草植株的叶绿素a,总叶绿素,类胡萝卜素和净光合速率（Pn）、胞间CO2浓度（Ci）等光合指标以及显著提高超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性,统计分析结果表明,各处理间差异显著（P〈0.05）。同样,钼营养显著增加了甘草株高、芦头直径、生物量等生长指标。其中,10.4mg·L-1钼处理时的甘草根鲜重和干重最大,与0 mg·L-1处理相比,分别显著增加了55.35%和38.08%。结论：钼不足会抑制甘草的各项生理功能,进而影响甘草的生长,而5.2mg·L-1和10.4mg·L-1的钼营养浓度可以促进一年生甘草各项生理和生长指标的增加,进而促进甘草干物质的积累,提高甘草药材的产量。     

钼营养对甘草生理和生长特性的影响

目的:探讨不同浓度的钼营养水平对甘草生长和生理特性的影响。方法:以一年生的甘草移栽苗为试验材料,采用盆栽蛭石的试验方法,共设置4个钼浓度水平,分别为0mg·L-1,0.52mg·L-1,5.2mg·L-1和10.4mg·L-1,其中0.52mg·L-1即正常Hoagland营养液中钼的浓度。每周向盆内浇灌营养液,以达到处理的目的。采用LI-6400光合仪测定其光合生理指标以及植物生理学常规方法进行甘草叶片色素和抗氧化酶活性的测定。采用电子天平分别测定不同处理下的甘草地上、根的鲜重和干重等。结果:结果表明,甘草的各项生理和生长指标随着钼处理浓度的增加而增加,显著增加了甘草植株的叶绿素a,总叶绿素,类胡萝卜素和净光合速率(Pn)、胞间CO2浓度(Ci)等光合指标以及显著提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,统计分析结果表明,各处理间差异显著(P<0.05)。同样,钼营养显著增加了甘草株高、芦头直径、生物量等生长指标。其中,10.4mg·L-1钼处理时的甘草根鲜重和干重最大,与0 mg·L-1处理相比,分别显著增加了55.35%和38.08%。结论:钼不足会抑制甘草的各项生理功能,进而影响甘草的生长,而5.2mg·L-1和10.4mg·L-1的钼营养浓度可以促进一年生甘草各项生理和生长指标的增加,进而促进甘草干物质的积累,提高甘草药材的产量。     

Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch)

The effects of salinity and drought on the antioxidative system (SOD, POD, CAT) were studied in liquorice seedlings (Glycyrrhiza uralensis Fisch). The results showed that both salt and drought stresses could induce oxidative stress, as indicated by the increase level of lipid peroxidation. The activities of SOD and POD were up-regulated by salt and drought stress, while CAT activity decreased. An additional MnSOD isoenzyme was detected in liquorice subjected to 2%NaCl stress. The data also showed that although the activity of SOD was differentially influenced by drought and salinity, the changes of antioxidant enzyme activities subjected to drought stress follow a pattern similar to that subjected to salt stress, indicating that similar defensive systems might be involved in the oxidative stress injury in liquorice.     

Differential responses of antioxidant enzymes to aluminum toxicity in two rice (Oryza sativa L.) cultivars with marked presence and elevated activity of Fe SOD and enhanced activities of Mn SOD and catalase in aluminum tolerant cultivar

Seedlings of two Indica rice (Oryza sativa L.) cvs. HUR-105 and Vandana, differing in Al-tolerance were used to identify the key mechanisms involved in their differential behaviour towards Al toxicity. Cv. HUR-105 appeared to be Al sensitive by showing significant reduction (p ≤ 0.01) in root/shoot length, fresh weight, dry weight and water content in presence of 421 μM Al³⁺ in growth medium whereas cv. Vandana appeared to be fairly Al³⁺ tolerant. A conspicuous and significant reduction in dry weight of root and shoot was observed in Al sensitive cv. HUR-105 with 178 μM Al³⁺ treatment for 3 days. Al was readily taken up by the roots and transported to shoots in both the rice cultivars. Localization of absorbed Al was always greater in roots than in shoots. Our results of the production of reactive oxygen species (ROS) H₂O₂ and O₂ ^.? and activities of major antioxidant enzymes such as total superoxide dismutase (SOD), Cu/Zn SOD, Mn SOD, Fe SOD, catalase (CAT) and guaiacol peroxidase revealed Al induced higher oxidative stress, greater production of ROS and lesser capacity to scavenge ROS in cv. HUR-105 than Vandana. With Al treatment, higher oxidative stress was noted in shoots than in roots. Greatly enhanced activities of SOD (especially Fe and Mn SOD) and CAT in Al treated seedlings of cv. Vandana suggest the role of these enzymes in Al tolerance. Furthermore, a marked presence of Fe SOD in roots and shoots of the seedlings of Al tolerant cv. Vandana and its significant (p ≤ 0.01) increase in activity due to Al-treatment, appears to be the unique feature of this cultivar and indicates a vital role of Fe SOD in Al-tolerance in rice.     

Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots

Effect of two Ni concentrations (10 and 200 μM) on growth, Ni accumulation, chlorophyll and proline contents, relative water content (RWC) as well as the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and glutathione S-transferase (GST) were studied in shoots of wheat plants. Treatments caused a considerable accumulation of Ni in the shoots. However, exposure of plants to 10 μM Ni did not lead to significant alterations in shoot growth except for a slight increase in fresh mass. The other parameters studied were not affected by treatment of plants with 10 μM Ni. In contrast, 200 μM Ni caused inhibition of shoot growth, a decline in RWC and chlorophyll content, accumulation of proline and occurrence of visible symptoms of Ni toxicity. The activities of SOD and CAT decreased in response to 200 μM Ni. Conversely, several-fold enhancements of POD and GST activities were observed following the 3^rd day of 200 μM Ni treatment.     

镍对高冰草幼苗生长及活性氧代谢的影响

本文对镍处理下高冰草幼苗生长,茎部和根部丙二醛(MDA)含量,可溶性蛋白含量,超氧化物岐化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性及叶片光合色素含量的变化进行了研究.结果表明,低浓度镍(10 μmol·L~(-1))对高冰草幼苗生长无显著抑制效应,但随镍浓度增加各项生长指标显著减少,且镍对高冰草幼苗根部损伤更为显著.光合色素含量随镍浓度升高呈减少趋势.高冰草茎部和根部MDA含量与镍浓度呈显著正相关关系.根部可溶性蛋白含量随镍浓度升高呈增加趋势.低浓度镍处理(10 μmol·L~(-1))下,SOD、POD和CAT活性均有所提高,保护酶系统处于正常状态,但随镍浓度升高,3种保护酶活性受到不同程度抑制,保护酶系统平衡被破坏.

Abstract：

Agropyron elongatum seedlings were exposed to different concentration Ni to study their growth and the changes in MDA and soluble protein contents and SOD, POD and CAT ac-tivities in their shoots and roots, and the photosynthetic pigment content in their leaves. Low con-centration (10 μmol·L~(-1)) Ni had no significant inhibitory effects on the seedlings growth, but the growth indices decreased significantly with increasing Ni concentration, being more markedly for roots than for shoots. The leaf photosynthetic pigment content had a decreasing trend with in-creasing Ni concentration, while the MDA content in shoots and roots had significant positive cor-relation with Ni concentration. The soluble protein content in roots increased with increasing Ni concentration. Under low concentration (10μmol·L~(-1)) Ni, the SOD, POD and CAT activities all increased, and the protective enzyme system was well-balanced. When the Ni concentration was increasing, the three enzyme activities were inhibited to different extents, and the balance of protective enzyme system was broken.     

Possible Involvement of Anti-Oxidant Enzymes in the Cross-Tolerance of the Germination/Growth of Wheat Seeds to Salinity and Heat Stress

The germination/growth of wheat （Triticum aestivum L. cv. Zimai 1） seeds and changes in the activities of superoxide dismutase （SOD）, ascorbate peroxidase （APX）, and catalase （CAT）, as well as in the content of thiobarbituric acid-reactive substances （TBARS）, in response to salt and heat stress, as well as cross-stress, were investigated in the present study. With increasing temperature and decreasing water potential caused by NaCI solution, the germination percentage of seeds and the fresh weight of seedlings decreased markedly, SOD activity increased, activities of APX and CAT decreased distinctly, and the TBARS content increased gradually. Seeds pretreated at 33℃ for different times displayed increased tolerance to subsequent salt stress, enhanced SOD, APX, and CAT activities, and decreased TBARS content. Seeds pretreated at -0.8 MPa NaCI for different times displayed increased tolerance to subsequent heat stress and marked increases in SOD, APX, and CAT activities, which were associated with decreased TBARS content. It is considered that the common component in the cross-tolerance of the germination and growth of wheat seeds to salinity and heat stress is the anti-oxidant enzyme system.     

铜污染对三叶草幼苗生长及活性氧代谢影响的研究

通过水培实验研究了重金属铜(Cu)污染对三叶草(Trifolium pratense)幼苗生长及活性氧代谢系统的影响。结果表明,低浓度Cu污染(<10mg·L^-1)对三叶草幼苗生长无明显抑制现象,甚至促进幼苗生长,植株干重、鲜重和叶片可溶性蛋白及叶绿素含量均略微升高,丙二醛(MDA)水平降低,活性氧清除系统内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性均略微升高,保护酶系统仍保持平衡.但随Cu浓度(10～100mg·L^-1)增加则显示出一定的负效应,三叶草幼苗与对照组相比,植株矮小,须根短且数目少,植株干重、鲜重和可溶性蛋白含量均明显减少,叶片发黄,叶片色素含量下降,并随Cu浓度的增加而变化更显著。同时,随Cu浓度增加,叶片细胞膜透性增大,电导率显著升高,MDA水平上升,且活性氧清除系统遭到破坏,保护酶系统失衡,SOD和CAT活性显著下降,分别降低了26．7％和71．3％,而POD活性却明显上升,比对照升高了10．6倍。     

硫肥对春油菜幼苗生理指标和土壤酶活性的影响

该研究以春油菜幼苗为材料,采用土壤盆栽试验,设7个不同施硫(0、35、70、105、140、175、210mg·kg^-1)处理,通过测定春油菜幼苗的株高、植株鲜重、叶绿素含量、MDA含量、SOD、POD、CAT活性、土壤全氮含量、pH、蔗糖酶、过氧化氢酶和脲酶活性指标,分析不同施硫量对春油菜幼苗生理生化指标和土壤相关酶活性的影响。结果表明:在春油菜苗期施用硫肥对幼苗的农艺性状、生理生化指标和土壤酶活性均产生了一定影响。施硫量在35~105mg·kg^-1范围时,对植株鲜重有明显的促进作用;施硫量在70~105mg·kg^-1范围时,类胡萝卜素含量达到最高;施硫量在70~105mg·kg^-1范围时,叶片中POD和CAT的活性明显升高,而MDA含量明显下降;经相关分析,MDA含量与POD活性呈极显著负相关(r=-0.92,P<0.01),与CAT活性呈显著负相关(r=-0.72,P<0.05),说明叶片MDA含量受POD和CAT活性变化的影响;施硫量高于105mg·kg^-1时,土壤脲酶和蔗糖酶活性受到抑制;施硫量高于140mg·kg^-1时,土壤过氧化氢酶活性受到抑制;随着施硫量的增加,土壤pH值和叶片SOD活性逐渐下降;经相关性分析,土壤脲酶活性和全氮含量间呈极显著正相关(r=1,P<0.01),表明土壤全氮含量受土壤脲酶活性变化的影响。由此可知,在低硫(35~105mg·kg^-1)条件下对春油菜幼苗生理生化指标及土壤酶活性具有一定的促进作用,而在高硫(>105mg·kg^-1)条件下则产生抑制。