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De novo analysis of the transcriptome of Pratylenchus zeae to identify transcripts for proteins required for structural integrity,sensation, locomotion and parasitism
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John Fosu‐Nyarko Jo‐Anne C. H. Tan Reetinder Gill Vaughan G. Agrez Uma Rao Michael G. K. Jones 《Molecular Plant Pathology》2016,17(4):532-552
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Watanabe S Minegishi Y Yoshinaga T Aoyama J Tsukamoto K 《Marine biotechnology (New York, N.Y.)》2004,6(6):566-574
To compensate for the limited number of morphological characteristics of fish eggs and larvae, we established a convenient and robust method of species identification for eggs of the Japanese eel (Anguilla japonica) using a real-time polymerase chain reaction (PCR) that can be performed onboard research ships at sea. A total of about 1.2 kbp of the mitochondrial 16S ribosomal RNA gene sequences from all species of Anguilla and 3 other anguilliform species were compared to design specific primer pairs and a probe for A. japonica. This real-time PCR amplification was conducted for a total of 44 specimens including A. japonica, A. marmorata, A. bicolor pacifica, and 6 other anguilliform species. Immediate PCR amplification was only observed in A. japonica. We then tested this method under onboard conditions and obtained the same result as had been produced in the laboratory. These results suggest that real-time PCR can be a powerful tool for detecting Japanese eel eggs and newly hatched larvae immediately after onboard sampling during research cruises and will allow targeted sampling efforts to occur rapidly in response to any positive onboard identification of the eggs and larvae of this species. 相似文献
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Hajime Muraguchi Kiwamu Umezawa Mai Niikura Makoto Yoshida Toshinori Kozaki Kazuo Ishii Kiyota Sakai Motoyuki Shimizu Kiyoshi Nakahori Yuichi Sakamoto Cindy Choi Chew Yee Ngan Eika Lindquist Anna Lipzen Andrew Tritt Sajeet Haridas Kerrie Barry Igor V. Grigoriev Patricia J. Pukkila 《PloS one》2015,10(10)
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Transcriptome sequencing and comparative analysis of the gametophyte thalli of Pyropia tenera under normal and high temperature conditions 总被引:1,自引:0,他引:1
San Choi Mi Sook Hwang Sungoh Im Namju Kim Won-Joong Jeong Eun-Jeong Park Yong-Gun Gong Dong-Woog Choi 《Journal of applied phycology》2013,25(4):1237-1246
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