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Differential transcription of Xenopus oocyte and somatic-type 5 S genes in a Xenopus oocyte extract 总被引:10,自引:0,他引:10
L Millstein P Eversole-Cire J Blanco J M Gottesfeld 《The Journal of biological chemistry》1987,262(35):17100-17110
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Xenopus 5S RNA genes exhibit a pattern of differential expression during development in which some members (oocyte-type) are transcribed only in oocytes, while others (somatic-type) are expressed in both oocytes and somatic cells. Using cloned DNA probes specific for each gene type, we determined the positions of these genes on Xenopus metaphase chromosomes by in situ hybridization. Somatic-type 5S genes in both X. laevis and X. borealis are located at the distal end of the long arm of only one chromosome (number 9). The oocyte-type 5S RNA genes are found at the distal ends of the long arms of most Xenopus chromosomes, including chromosome 9. Thus, large scale differences in chromosomal location cannot explain the selective expression of these genes, as suggested previously. 相似文献
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Transcription fraction TFIIIC can regulate differential Xenopus 5S RNA gene transcription in vitro 总被引:30,自引:4,他引:26
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A P Wolffe 《The EMBO journal》1988,7(4):1071-1079