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Nakazawa M Ichikawa T Ishikawa A Kobayashi H Tsuhara Y Kawashima M Suzuki K Muto S Matsui M 《The Plant journal : for cell and molecular biology》2003,34(5):741-750
In a screen for morphological mutants from the T1 generation of approximately 50 000 activation-tagging lines, we isolated four dominant mutants that showed hyponastic leaves, downward-pointing flowers and decreased apical dominance. We designated them isoginchaku (iso). The iso-1D and iso-2D are allelic mutants caused by activation of the AS2 gene. The T-DNAs were inserted in the 3' downstream region of AS2. Iso-3D and iso-4D are the other allelic mutants caused by activation of the ASL1/LBD36 gene. These two genes belong to the AS2 family that is composed of 42 genes in Arabidopsis. The only recessive mutation isolated from this gene family was of AS2, which resulted in a leaf morphology mutant. Applying reverse genetics using a database of activation-tagged T-DNA flanking sequences, we found a dominant mutant that we designated peacock1-D (pck1-D) in which the ASL5/LBD12 gene was activated by a T-DNA. The pck1-D mutants have lost apical dominance, have epinastic leaves and are sterile. These results strongly suggest that activation tagging is a powerful mutant-mining tool especially for genes that make up a gene family. 相似文献
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在拟南芥(Arabidopsis thaliana (L.) Heynh.)叶发育研究中,as2是一个经典突变体.as2典型的表型是叶片开裂或形成一种小叶状结构.遗传学和分子生物学实验证明,AS2基因具有抑制KNOX基因在叶中表达的功能.在本文中,我们着重研究了新得到的在Landsberg erecta (Ler)遗传背景下的as2突变体.除了前人报道过的as2表型外,新as2突变体的部分叶柄长在叶片的下方,形成一种荷叶状结构,更严重的甚至长成花丝状叶结构.这两种结构都反映了不正常的叶腹背轴极性分化.在我们所收集到的as2等位突变体中,只有在Ler背景下这两种结构才以高频率出现.我们通过图位克隆方法分离了AS2基因.该基因编码一个含有亮氨酸拉链结构的蛋白.在拟南芥中,AS2同源基因共43个,除AS2外,其他基因的功能都不清楚.AS2在叶和花中表达,在茎中无表达,这种表达模式和as2突变体的表型是吻合的. 相似文献
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Zhongfei Li Bin Li Jian Liu Zhihao Guo Yuhao Liu Yan Li Wen‐Hui Shen Ying Huang Hai Huang Yijing Zhang Aiwu Dong 《植物学报(英文版)》2016,58(12):959-970
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拟南芥编码亮氨酸拉链蛋白的AS2基因在叶发育中具有控制极性建立的作用 总被引:5,自引:0,他引:5
在拟南芥 (Arabidopsisthaliana (L .)Heynh .)叶发育研究中 ,as2是一个经典突变体。as2典型的表型是叶片开裂或形成一种小叶状结构。遗传学和分子生物学实验证明 ,AS2基因具有抑制KNOX基因在叶中表达的功能。在本文中 ,我们着重研究了新得到的在Landsbergerecta (Ler)遗传背景下的as2突变体。除了前人报道过的as2表型外 ,新as2突变体的部分叶柄长在叶片的下方 ,形成一种荷叶状结构 ,更严重的甚至长成花丝状叶结构。这两种结构都反映了不正常的叶腹背轴极性分化。在我们所收集到的as2等位突变体中 ,只有在Ler背景下这两种结构才以高频率出现。我们通过图位克隆方法分离了AS2基因。该基因编码一个含有亮氨酸拉链结构的蛋白。在拟南芥中 ,AS2同源基因共 4 3个 ,除AS2外 ,其他基因的功能都不清楚。AS2在叶和花中表达 ,在茎中无表达 ,这种表达模式和as2突变体的表型是吻合的。 相似文献
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Masaya Ikezaki Mikiko Kojima Hitoshi Sakakibara Shoko Kojima Yoshihisa Ueno Chiyoko Machida Yasunori Machida 《The Plant journal : for cell and molecular biology》2010,61(1):70-82
The asymmetric leaves 1 ( as1 ) and as2 mutants of Arabidopsis thaliana exhibit pleiotropic phenotypes. Expression of a number of genes, including three class-1 KNOTTED -like homeobox ( KNOX ) genes ( BP , KNAT2 and KNAT6 ) and ETTIN / ARF3 , is enhanced in these mutants. In the present study, we attempted to identify the phenotypic features of as1 and as2 mutants that were generated by ectopic expression of KNOX genes, using multiple loss-of-function mutations of KNOX genes as well as as1 and as2 . Our results revealed that the ectopic expression of class-1 KNOX genes resulted in reductions in the sizes of leaves, reductions in the size of sepals and petals, the formation of a less prominent midvein, the repression of adventitious root formation and late flowering. Our results also revealed that the reduction in leaf size and late flowering were caused by the repression, by KNOX genes, of a gibberellin (GA) pathway in as1 and as2 plants. The formation of a less prominent midvein and the repression of adventitious root formation were not, however, related to the GA pathway. The asymmetric formation of leaf lobes, the lower complexity of higher-ordered veins, and the elevated frequency of adventitious shoot formation on leaves of as1 and as2 plants were not rescued by multiple mutations in KNOX genes. These features must, therefore, be controlled by other genes in which expression is enhanced in the as1 and as2 mutants. 相似文献
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Jiro Yamada 《Bioscience, biotechnology, and biochemistry》2013,77(5):1269-1270
Two indole alkaloids which induce the Epstein-Barr virus early antigen of Raji cells (B lymphocyte) were found in the cultured broth of Actinomycetes NA34-17, from which teleocidin B was also obtained. The active compounds isolated were identified from their spectral data and chemical evidence as (—)-indolactam V and (—)-14-O-acetyl indolactam V. 相似文献
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Why don't mosses flower? 总被引:4,自引:0,他引:4
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目的:探讨S-亚硝基-N-乙酰-DL-青霉胺(SNAP)对巨噬细胞亚型分化的影响及其机制。方法:以RAW264.7巨噬细胞为研究对象,分为空白对照组、SNAP组、SNAP+PBA(4-苯基丁酸)组,采用不同浓度(30、100、300、400、500μmol/L)的SNAP或300μmol/L SNAP+20 mmol/L PBA对巨噬细胞进行干预24 h,应用RT-PCR法检测RAW264.7巨噬细胞亚型分化标志物M1(iNOS,CD86)、M2(Arg-I,MR)及CHOP mRNA的表达,应用Western blot技术检测iNOS及ERS通路中相关蛋白CHOP、P-PERK的表达。结果:与空白对照组比较,SNAP组iNOS、CD86、CHOPmRNA的表达均明显降低(P0.05),Arg-ImRNA表达明显升高(P0.05),而MR mRNA表达升高,但差异无统计学意义(P0.05);与300μmol/L SNAP组比较,300μmol/L+PBA组iNOS、CHOP mRNA均无明显变化(P0.05),CD86 mRNA升高,Arg-I、MR mRNA均明显降低(P0.05)。SNAP组CHOP、iNOS、p-PERK蛋白表达均明显低于对照组(P0.05),300μmol/LSNAP+20 mmol/LPBA组与300μmol/LSNAP组比较iNOS蛋白、p-PERK、CHOP蛋白表达升高(P0.05)。结论:NO可能通过内质网应激机制抑制巨噬细胞向M1亚型分化。 相似文献
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Dou-Dou Chen Liang-Liang Hui Xiang-Cheng Zhang Qing Chang 《Journal of cellular biochemistry》2019,120(2):2493-2501
Long noncoding RNAs (lncRNA) have been recognized as significant regulators in the progression of atherosclerosis (AS). Oxidized low-density lipoprotein (ox-LDL) can induce macrophage inflammation and oxidative stress, that serves important roles in AS. However, the exact function of lncRNA NEAT1 and its possible molecular mechanism in AS remain unclear. Here, we concentrated on the roles and molecular mechanisms of NEAT1 in AS development. In our current study, we observed that NEAT1 was elevated by ox-LDL in a dose-dependent and time-dependent manner. RAW264.7 cell survival was greatly enhanced, and cell apoptosis was significantly inhibited by LV-shNEAT1 transfection. In addition, knockdown of NEAT1 in RAW264.7 cells repressed CD36 expression and foam cell formation while NEAT1 overexpression shown an opposite process. Moreover, NEAT1 downregulation inhibited inflammation molecules including IL-6, IL-1β, and TNF-α. Meanwhile, silencing of NEAT1 can also suppress reactive oxygen species (ROS) and malondialdehyde (MDA) levels with an enhancement of superoxide dismutase (SOD) activity in RAW264.7 cells. MicroRNAs are some short RNAs, and they can regulate multiple biological functions in many diseases including AS. Here, we found that miR-128 expression was remarkably decreased in ox-LDL-incubated RAW264.7 cells. Interestingly, miR-128 mimics was able to reverse AS-correlated events induced by overexpression of NEAT1. By using bioinformatics analysis, miR-128 was predicted as a target of NEAT1 and the correlation between them was validated in our study. Taken these together, it was implied that NEAT1 participated in ox-LDL-induced inflammation and oxidative stress in AS development through sponging miR-128. 相似文献