Defective Interfering Particles of Vesicular Stomatitis Virus: Functions of the Genomic Termini

The functions of thecis-acting sequence elements at the termini of the negative strand RNA virus vesicular stomatitis virus and its defective-interfering particles were evaluated. Either genomic terminus could signal replication but increasing the complementarity of the termini enhanced replication irrespective of whether the termini consisted of the 3′ leader and its complement or the 5′ trailer and its complement. The 5′ trailer region contains an essentialcis-acting requirement for assembly of RNPs into infectious particles. These findings explain why the majority of DI RNAs are of the 5′ copy-back class: RNAs with complementary termini from either end have a replicative advantage, but only 5′ copy-back RNAs contain the signal for assembly into particles.     

针对leader序列的siRNA能够有效抑制SARS冠状病毒多个mRNA的表达

小干扰RNAs(siRNAs)能够有效降解具有互补序列的RNA.在SARS-CoV的基因组RNA和所有亚基因组RNA的5′端均有一段共同的leader序列,而且该leader序列在不同的病毒分离物中高度保守,因此leader序列可作为一个用于抑制SARS-CoV复制的有效靶点.研究表明,针对leader序列化学合成的siRNA和DNA载体表达的shRNA都可以有效抑制SARS-CoV mRNA的表达.Leader序列特异的siRNA或shRNA不仅可以有效抑制leader与报告基因EGFP融合基因的表达,而且还可以有效抑制leader与刺突蛋白(spikeprotein)、膜蛋白(membrane protein)和核衣壳蛋白(nucleocapsid protein)基因的融合转录产物的表达.结果表明,针对leader序列的RNA干扰可以发展成为一种抗SARS-CoV治疗的有效策略.     

Assembly of the Particle of Tobacco Mosaic Virus from RNA and Disks of Protein

The reconstitution of TMV does not proceed by the stepwise addition of single protein subunits, but by the addition of preformed disks to the growing rod. The assembly is initiated by the interaction of a disk with a special sequence of about fifty nucleotides at the 5′ end of the TMV RNA. This is the basis of the selectivity for viral over other RNAs.