Parasitic deuteromycetes from South Brazil

Summary This paper deals with four new fungi from the State of Rio Grande do Sul, Brazil.Phaeseptoria stenocalycis Batista, Upadhyay &Da Costa Netto, collected on leaves ofStenocalyx dasyblastus Bert. from Grammado,Phoma jasmino-macrospora Batista, Upadhyay &Da Costa Netto, collected on leaves ofJasminum flexicaule Vahl. from Pôrto Alegre,Melanconium argutidentis Batista, Upadhyay &Da Costa Netto, collected on leaves ofSebastiana argutidens Pex &K. Hoff from Grammado andCercospora caleifoleii Batista, Upadhyay &Da Costa Netto, collected on leaves ofCalea pinnatifida Banks from Pôrto Alegre are described here.Symptoms on the hosts and morphological characters of the fruitings and spores are described and illustrated.Publicação no 456 of IMUFP.     

The effects of culture media on Saprolegnia antigens

In a previous paper (Hodkinson &Hunter, 1970a) we reported that salmon, suffering from a disease called ulcerative dermal necrosis, appeared to have precipitating antibodies to the mycelium of a fungus associated with their diseased skin lesions. This was confirmed when antigens were prepared from pure cultures of the fungus, a species ofSaprolegnia (Hodkinson &Hunter, 1970b). In this paper we describe the effects of culture media on the antigenicity ofSaprolegnia mycelium, as detected by double gel diffusion with salmon sera.     

A stable mitomycin-induced LC-type albino mutant of Blastocladiella emersonii

In an effort to obtain orange mutants ofBlastocladiella emersonii Cantino &Hyatt, wild type zoospores were treated with mitomycin. From the variants produced, we obtained a stable, albino mutant (Ma-1) that differs significantly from another, previously described (Shaw &Cantino, 1969) UV-induced, albino variant. This report concerns the origin of Ma-1, its distinguishing features, and its apparent similarity to the few LC (late colorless) plants that normally appear in wild type populations. A preliminary note regarding mitomycin-induced variants ofB. emersonii has been published (Matsumae &Cantino, 1970).     

Nutritional studies on species and mutants of Lepista,Cantharellus, Pleurotus and Volvariella

The purpose of this study is to determine the ability of select Agaricales species to utilize various sources of carbon, nitrogen, vitamins, and growth hormones. Fungi selected for the studies include:Cantharellus clavatus Fries,C. cibarius Fries,Lepista nuda (Bull. ex. Fries)Cooke,Pleurotus ostreatus (Jacq. ex. Fries)Kummer, andVolvariella volvacea (Bull. ex. Fries)Singer. Three strains ofC. cibarius and one mutant ofV. volvacea (V135), V134, were employed to determine if nutritional requirement differences occurred. One species,V. volvacea, is grown commercially as a cottage industry in the Orient (Alicbusan &Ela, 1961) while the other species currently have no commercial value. All species studied possess pleasing flavors and have potential use in the mushroom production industry.A literature compilation of the nutritional regulation of basidiocarp formation and vegetative growth of Agaricales was made with specific mention to the named species (Volz &Beneke, 1969). Recent nutritional studies with one or more of the specific species include those byYusef &Allam (1967), andEger (1970).     

Fungi causing plant diseases at jabalpur (madhya pradesh)-IX

Summary The present paper describes 6 foliicolous fungi from Jabalpur (India). These includeAsterina lawsoniae Syd. on leaves ofLawsonia alba, Diplocarpon rosae Wolf. onRosa sp.,Cercospora jujubae Chowdhury onZizyphus jujuba andCercospora subsessilis Henn. &Nym. onAzadirachta indica, new fungus records for Madhya Pradesh;Asterostomella strophanti Henn. on leaves ofFlacourtia ramontchii, a new fungus for the country andCorynespora cassiicola (Berk. &Curt.)Wei. onClerodendron inerme, a new host record.     

Novos Deuteromycetes da micogeografia intercontinental

Summary This paper deals with some interesting new fungi which are described as a contribution for the development of the Mycogeography.Arbuscula Batista &Peres n. gen. is studied as a member of the family Stilbaceae havingA. eugeniae Batista &Peres n. sp. on leaves ofEugenia jambolana Lam. from Lucknow, India, as the type species. Another new genus isEllisia Batista &Peres, typified byE. ingae Batista &Peres n. sp. on leaves ofInga fagifolia (L.)Willd. from Manaus. Amazonas, Brazil. Two new binomia are:Eriomycopsis paraensis Batista &Peres n. sp. on leaves ofBertholletia excelsa H.B.K. from Belem, State of Pará, Brazil,Sporidesmium americanense Batista &Peres n. sp. on leaves ofQuercus wislizenii A.D.C., from U.S.A. andSporidesmium cookei (Hughes)W. B. Ellis, on leaves of Lauraceae, from Manaus.

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Zusammenfassung Diese Arbeit bringt einige interessante neue Pilze, welche als Beitrag zur Entwicklung der Mycogeographie beschrieben werden.Arbuscula Batista &Peres n. gen. wurde studiert als ein Mitglied der Familie Stilbaceae, dessen TypusA. eugeniae Batista &Peres n. sp. sich auf Blättern vonEugenia jambolana Lam. aus Lucknow, Indien, befand. Eine andere neue Gattung istEllisia Batista &Peres, typiziert durchE. ingae Batista &Peres n. sp. auf Blättern vonInga fagifolia (L.)Willd. aus Manaus, Amazonas, Brasilien. Zwei neue Binome sind:Eriomycopsis paraensis Batista &Peres n. sp. auf Blättern vonBertholletia excelsa H.B.K. aus Belém, Staat Pará, Brasilien,Sporidesmium americanense Batista &Peres n. sp. auf Blättern vonQuercus wislizenii A.D.C. aus U.S.A. undSporidesmium cookei (Hughes)W. B. Ellis, auf Blättern von Lauraceae, aus Manaus, Amazonas.

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Dedicado à Mrmória do Prof.Raffaele Ciferri     

Relationships ofJusticieae (Acanthaceae) based on cytology

The eytology of some species representing the subtribesBarlerieas, Andrographideae, Eujusticieae andDicliptereae of theJusticieae ofBentham & Hooker, suggests thatBarlerieae are the most primitive subtribe. Separation ofBarlerieae in Engler's system from the tribeJusticieae has been supported. But separation ofEcbolium andRungia in a different taxon,Odontonemeae in the classification ofEngler does not find justification. The position ofRungia underDicliptereae (Bentham & Hooker) is supported.     

Studies on Aspergilli XVI. Effect of pH,temperature, and carbon and nitrogen interaction

Summary The effect of pH, temperature, and carbon and nitrogen interaction on the growth and sporulation ofAspergillus nidulans (Eidam)Wint.,A. rugulosus Thom &Raper,A. variecolor (Berk. &Br.)Thom &Raper andA. quadrilineatus was studied. All the moulds could grow on a wide range of pH (2.0 to 12.0) but the growth was poor on too acid and too alkaline media. Best growth ofA. rugulosus, A. quadrilineatus, andA. violaceus was seen at pH 6.5 and that ofA. nidulans andA. variecolor at pH 7.0. In general maximum production of perithecia was recorded between pH 6.0 and 8.0.All the above species ofAspergillus under study could grow between a temperature range of 10° C–48° C, but the growth was poor at 10° C and 48° C. The present moulds showed good growth at 20° C, 25°C, and 30° C. At 40° CA. nidulans andA. rugulosus showed moderate growth while the rest of the Aspergilli attained good growth. Temperatures between 20° C–30° C favoured excellent perithecial production.In general, little improvement in growth was noted on media containing good carbon and nitrogen sources. Malic acid was found to be useless when supplied singly. But, poor growth was recorded when supplied in combination with amino acids, amide, and peptone. This was due to the fact that these N sources also supplied carbon for their metabolism.     

The mitotic configuration of Chaetomium globosum

The somatic nuclear division ofChaetomium globosum was studied utilizing acetocarmin and aceto-orcein techniques. Nuclear division in hyphae of this species was found to be mitotic, but diversity in morphology and division configuration was noted. Identifiable chromosomes, the metaphase plate, and the chromosome bridge were commonly seen.A combination of extremely small nuclei, difficulties in staining, multinucleate conditions, and protoplasmic streaming in hyphae presented difficulties for these studies. Contradictory views are held on the structure of the nucleus, presence of the centriolar body, and other karyological features as described byFinley (1970)Rabinow &Bakerspigel (1965). Nuclear division structural details in a few other fungal species such as the centriolar body, spindle apparatus, and nuclear membrane disassociation have been examined by electron microscopy (Motta, 1969;Ichida &Fuller, 1968;Namboodiri &Lowry, 1967).The present study and the previous report onC. globosum (Hsu, Yu &Volz, 1972) presents comparative data for a NASA Appollo 16 MEED Mycology investigation now in progress.     

The oldest Jurassic cyathophorid coral (Scleractinia) from siliciclastic environments of the Kachchh Basin, western India

Cyathophora Michelin, 1843, hitherto well known from the Upper Jurassic and Cretaceous, has been found in the Middle Jurassic (Bajocian) of the Kachchh Basin, western India. Eleven specimens ofCyathophora bourgueti (Defrance, 1826) from the Babia Cliff Sandstone member of the Kaladongar Formation, exposed along the northern scarp of the Kala Dongar, Pachchham Island, Kachchh, are described and illustrated as the earliest Jurassic record of the family CyathophoridaeVaughan & Wells, 1943. It is suggested that the monospecific occurrence ofCyathophora bourgueti was controlled by salinity.      

Paleobotanical studies inFagaceae of the European Tertiary

A systematic reassessment of megafossil records ofFagaceae in Central Europe has been undertaken on the basis of leaf cuticular characters. The oldest representatives date back to the Eocene:Quercus subhercynica spec. nova,Dryophyllum furcinerve (Rossm.)Schmalh.,Trigonobalanopsis rhamnoides (Rossm.) gen. & comb. nov. In the Oligocene other members of extant genera appear:Quercus rhenana (Weyl. & Kilpp.)Knobloch & Kvaek,Fagus attenuata Goepp.,Lithocarpus saxonicus spec. nova. In the Neogene these ancient taxa (except inFagus lineage), are gradually replaced by deciduous species ofQuercus andCastanea. Trigonobalanus andCastanopsis are recorded by fruits (or wood) only.     

Chrysosphaerella multispina Bradley and some other remarkableChrysophyceae from Lake Stråken,Aneboda, Sweden

Summary Chrysophyceae from Lake Stråken, Aneboda, Sweden, have been examined by electron microscopy. 10 species ofChrysophyceae were found, and electron micrographs of their silica scales are given. 6 of these taxa have not previously been recorded from Sweden, viz.Chrysosphaerella multispina Bradley,Mallomonas crassisquama (Asmund)Fott,Mallomonas papillosa Harris &Bradley,Synura echinulata Korshikov, andSynura spinosa Korshikov f.spinosa Petersen &Hansen.Dedicated to Professor L.Geitler on the occasion of his 70th birthday.     

The Miocene Architectonicidae (Gastropoda) of Chile

Three new species of Architectonicidae Heliacinae,Heliacus (Torinista) chonos, Heliacus (Grandeliacus) antu andSolatisonax bieleri, are described. These include the first reports ofGrandeliacus andSolatisonax from Chile.Solarium australe Philippi, 1887 is an earlier name forHeliacus (Torinista) bahamondei Frassinetti & Covacevich, 1981, but is a junior homonym ofS. australe Philippi, 1849. Type material and new specimens of the five previously described architectonicid species from Chile are described and figured for comparison. Together with other gastropod taxa from the same deposits, Architectonicidae provide evidence for tropical to subtropical water temperatures in central Chile during the early Miocene.     

The utilization of the mixtures of monosaccharides by some mucorales

Summary The utilization of mixtures of monosaccharides byBlakeslea trispora Thaxter,Choanephora circinans (Naganish &Kawakami)Hesseltine &Benjamin,Gilbertella persicaria var.indica Mehrotra &Mehrotra andHelicostylum piriforme Bainier was studied. The effect of sorbose on the utilization of other sugars present in the mixtures was also studied. It was found that all the mixtures of sugars in combination with asparagine or ammonium chloride were valueless for all the organisms exceptHelicostylum piriforme. Growth ofHelicostylum piriforme on the mixtures with asparagine as the nitrogen source was better than on the mixtures with ammonium chloride as the source of nitrogen. Asparagine being a favourable source counteractecd sorbose inhibition, while ammonium chloride failed to do so. On the other hand, both of the nitrogen sources failed to counteract sorbose inhibition in the rest of the organisms. None of the organisms could finish sorbose and rhamnose from any of the mixtures within the specified period.     

Antigenic relationships between Aracoccidioides loboi and other pathogenic fungi determined by immunofluorescence

Summary The fluorescent antibody technique was used to study antigenic relationships betweenParacoccidioides loboi and other pathogenic fungi. The findings suggest thatP. loboi is more closely related antigenically to certainP. brasiliensis strains than to others and that it has antigens in common with the yeast form ofHistoplasma capsulatum, H. duboisii, Blastomyces dermatitidis, Candida albicans and also the mycelial form ofCoccidioides immitis. Serum globulins from 3 cases of keloidal blastomycosis were labelled with fluorescein isothiocyanate. These conjugates showed slight or no reactivity withP. loboi, the yeast forms ofP. brasiliensis, H. capsulatum, H. duboisii andB. dermatitidis, However, they stained brightlyC. albicans, serotypes A and B, the tissue form ofC. immitis and the yeast form ofSporotrichum schenckii. Adsorption of these reagents withC. albicans eliminated all staining except that forS. schenckii. These patients had no history of clinical sporotrichosis.Deceased. Last address: Fundacão Gonçalo Moniz, Salvador, Bahia, Brazil. Requests for reprints should be sent to Dr.William Kaplan.Dr.Miranda is in private practice in Rio de Janeiro, Brazil.     

Zwei neue Arten der GattungSaponaria (Caryophyllaceae) aus Afghanistan und Pakistan

Saponaria stenopetala sp.n. in Eastern Afghanistan is close toS. pachyphylla Rech. f. andS. subrosularis Rech. f.—The nearest allies ofS. makranica sp.n. from Western Pakistan and Southeastern Iran areS. kermanensis Bornm. andS. floribunda (Kar. & Kir.)Boiss.

Flora Iranicae praecursores 36–37. — Praecursores praecurrentes in Pl. Syst. Evol.139, 313–317 (1982).     

Studies on the early floral development inCleomoideae (Capparaceae) with emphasis on the androecial development

Floral ontogeny ofCleome spinosa, Cleome violacea andPolanisia dodecandra subsp.trachysperma was studied in the context of the question whether the fascicled androecium ofReseda andCapparis (with fused fascicles) or the 2 + 4-pattern of theBrassicaceae is primitive in theCapparales. InPolanisia dodecandra, the 9–18 stamens show unidirectional initiation from the adaxial side toward the abaxial side of the flower. InCleome violacea, the six stamens also are formed in an unidirectional order, but development starts abaxially and a zigzag-like pattern is superimposed. InCleome spinosa, two stamen primordia in transversal (lateral) position are followed by four stamens which arise on a somewhat higher level in two pairs in front of the median sepals. It is assumed that the evolutionary steps in the androecial development proceed fromReseda viaCapparis andPolanisia/Cleome toBrassicaceae. This interpretation is supported byrbcL-studies (Chase & al. 1993,Rodman & al. 1993).Dedicated to emer. Univ.-Prof. DrFriedrich Ehrendorfer on the occasion of his 70th birthday     

Two remarkable newCruciferae from Saudi Arabia

A new genus ofCruciferae, Dolichorhynchus Hedge & Kit Tan (D. arabicus Hedge & Kit Tan) and a new speciesArabidopsis erysimoides Hedge & Kit Tan, both from northern Saudi Arabia, are described and illustrated. An addendum lists new Crucifer generic or species records for the Arabian peninsula.     

Newly reported fungi from Orissa (India) — I

Summary Two species of fungi,Tryblidiella rufula (Spreng.)Sacc. andTrabutia butleri Theiss &Syd. have been figured and described for the first time from Orissa. The former was found growing on dead twigs ofCitrus aurantifolia (Christm.)Swingle and onCitrus sp. and the latter on living leaves ofFicus religiosa L.     

Ecological study of soil fungi of an agricultural field in Allahabad

Summary The soil fungi from an agricultural field in Allahabad where sugarcane is being grown for many years, have been isolated from various depths during different seasons and were identified. The inter-relations of chemical composition of soil and distribution of fungi is also being shown here.The techniques for the isolation and the study of the fungus flora was that ofGoddard modified bySaksena &Mehrotra. Soil samples were examined from 1–6 depths in three seasons of the year and were mechanically and chemically analysed.For the isolation, soil dilution plate method, a modification ofMenzies' method, direct method ofWaksman, Rossi Cholodny Burried slide technique, and screened immersion plate method, were followed Fifty five different species of microfungi belonging to Phycomycetes, Ascomycetes and Fungi — Imperfecti were isolated and identified. The moisture contents, hydrogen-ion concentrations, carbon, nitrogen, phosphorus, calcium, magnesium and iron, etc., of the soil samples from 1–6 inches depths, were also studied.Out of these 12 species covering 9 genera belonged to the Phycomycetes, nine genera of Ascomycetes and nine genera of Fungi Imperfecti were recorded.P. multicolor Grigorieva-Monoilova &Poradielova, andP. roqueforti Thom.,Gliocladium vermoesoni (Biourge)Thom. andMasoniella grisea (Smith)Smith were recorded for the first time from Indian Soil. A new varietyChaetominum nigricolor Ames var.simplex was also isolated.