VEGF 和MMP-9 在肝癌病理诊断中的应用价值

目的:探讨血管内皮生长因子(VEGF)和基质金属蛋白酶-9(MMP-9)在原发性肝癌(PHC)组织中的表达及病理诊断价值。方法:采用免疫组化法,检测80例PHC标本和30例正常组织中VEGF和MMP-9的表达。结果:PHC组VEGF和MMP-9阳性表达率分别为68.8%、62.5%,均显著高于对照组(23.3%、26.7%),相比较有显著性差异(P<0.05)。肿瘤直径≥5 cm、无包膜和低分化肿瘤组织中VEGF和MMP-9阳性表达均显著高于肿瘤直径<5 cm、包膜完整和高中分化肿瘤组,相比较有显著性差异(P<0.05);而VEGF和MMP-9阳性表达在不同性别和年龄构成之间、有癌栓组和无癌栓组之间、AFP阳性组和阴性组之间差异无统计学意义(P>0.05)。结论:VEGF和MMP-9在PHC组织中呈高表达,VEGF和MMP-9可以作为PHC的预后指标。     

Unloading the infarcted heart affect MMPs–TIMPs axis in a rat cardiac heterotopic transplantation model

Ventricular assist devices may function as a bridge to recovery or heart transplantation, however, little is known about its mechanisms. This study examined the role of matrix metalloproteinases (MMP)-tissue inhibitors of metalloproteinases (TIMP) axis in the process of recovery after unloading in a rat ischemic-induce heart failure (HF) model. Myocardial infarction model was created with the coronary artery ligation. The infarcted rats hearts were unloaded by heterotopic cardiac transplantation (n = 14). 2 weeks later, the function of normal and infarcted hearts with or without loading was evaluated by Langendorff perfusion model. The hearts were then harvested and prepared for the study of expression of MMPs and TIMPs. Developed pressure in the unloading group was higher than the loading group (P = 0.0074). Unloading increased the ratio of TIMP-1–MMP-1(1.38 ± 0.11 vs. 0.76 ± 0.09, P < 0.05), TIMP-2–MMP-2 (1.06 ± 0.10 vs. 0.33 ± 0.07, P < 0.01), TIMP-3–MMP-9(1.07 ± 0.08 vs. 0.59 ± 0.06, P < 0.05). Although MMP-1, 2, 9 were downregulated (P < 0.01, 0.01, 0.05, respectively), TIMP-2 and TIMP-3 upregulated (P < 0.01, 0.05, respectively), MMP-7 and TIMP-1 was not affected significantly. The infarcted cardiac function could be improved by unloading. It was attributed to downregulation of MMP-1, 2 and 9, and upregulation of TIMP-2 and -3, and furthermore, the ratio of TIMPs to MMPs was increased, which might be more sensitive than sole MMPs or TIMPs for the judgment of myocardial matrix homeostasis.     

Effect of low-intensity pulsed ultrasound on MMP-13 and MAPKs signaling pathway in rabbit knee osteoarthritis

We evaluated the effect of low-intensity pulsed ultrasound (LIPUS) on MMP-13 and MAPKs expression in rabbit knee osteoarthritis (OA). For this purpose, 18 New Zealand white rabbits were randomly and equally divided into O + L, O – L, and SO groups. In O + L group, animals underwent right back leg ACLT operation and LIPUS radiation. In O − L group, animals underwent ACLT but no LIPUS treatment. In SO (control) group, animals underwent sham operation without LIPUS. After 6 weeks, we assessed the pathologic changes in the articular surface of femoral condyle and compared using Mankin scores. Also, expression of type-II collagen, MMP-13, ERK1/2, p38, and JNK was measured by Western blot. Compared with controls, Mankin scores were higher in O + L (P < 0.05)/O − L (P < 0.01) groups. Compared with O + L group, score was higher in O − L group (P < 0.05). Compared with controls, type-II collagen expression was less in O + L/O − L groups, with more significant decrease in O − L group (P < 0.05). Contrarily, expression of MMP-13, p-ERK1/2, and p-p38 was enhanced in O + L/O − L groups as compared with controls, with more significant increase in O − L group (P < 0.01). Compared with O + L group, expression was higher in O − L group (P < 0.05). We, therefore, concluded that LIPUS application promoted cartilage repair in OA through the downregulation of MMP-13, ERK1/2, and p38.     

Increase in extracellular cross-linking by tissue transglutaminase and reduction in expression of MMP-9 contribute differentially to focal segmental glomerulosclerosis in rats

Tissue transglutaminase (tTG) is a Ca²⁺-dependent enzyme which stabilizes the extracellular matrix (ECM) through post-translational modification, and may play an important role in the pathogenesis of focal and segmental glomerulosclerosis (FSGS). Here, we have investigated whether tTG contributes to the glomerular ECM expansion in the puromycin aminonucleoside (PAN)-injection-induced experimental rat model of FSGS. The localization and expression of tTG, MMP-9 gelatinase, and the ECM component fibronectin (FN) in kidneys was determined by immunohistochemistry and measured by semi-quantitative analysis. Protein levels of tTG and MMP-9 were also analyzed by Western blotting.In situtransglutaminase activity was assayed by measurement of incorporated substrate and the immunofluorescence staining for the cross-linking product, ε-(γ-glutamyl) lysine. Prominent proteinuria, a typical pathological feature of FSGS, was observed in PAN injection group rats. tTG immunoreactivity was located markedly in glomeruli and the levels of this protein in whole-kidney homogenates of PAN injection group rats were significantly increased (361± 106% control, P< 0.05). Similarly, transglutaminase activity and ε-(γ-glutamyl) lysine were also predominately located within glomeruli and were much more intense in the PAN-injected group than that in control animals. MMP-9 was also located primarily within glomeruli. In PAN-injected kidneys, protein levels of active MMP-9 were significantly reduced (59± 27% control, P< 0.01), while pro-MMP-9 levels increased (148± 42% control, P< 0.05). Remarkable expression of glomerular fibronectin (FN) was found in PAN injection group rats. Semi-quantitative analysis demonstrated this increased intensity of FN staining in the PAN-injected rats was 149± 23% of the control values (P< 0.05). Enhanced cross-linking of ECM by tissue transglutaminase and decreased degradation due to reduced active MMP-9 expression may be at least partially responsible for the deposition of FN within injured glomeruli in experimental FSGS.     

Expression of cytokeratin 19 and matrix metalloproteinase 2 predicts lymph node metastasis in hepatocellular carcinoma

The purpose of this study was to assess the value of cytokeratin 19 (CK19) and matrix metalloproteinase 2 (MMP-2) in predicting lymph node metastasis (LNM) and survival after curative resection in hepatocellular carcinoma (HCC) patients. Expression of CK19 and MMP-2 in tumor tissue was assessed through immunohistochemical staining of tissue microarrays (TMAs), which were constructed using samples from HCC patients with (n = 123) and without (n = 145) LNM. Positive CK19 expression was correlated with LNM (P < 0.001), satellite lesions (P = 0.016), and lymph node location (P = 0.039). High MMP-2 expression correlated with LNM (P < 0.001), UICC T stage (P = 0.023), and Edmondson grade (P = 0.022). Moreover, CK19 expression correlated with MMP-2 expression (P = 0.033). CK19 and MMP-2 expression were predictive of HCC LNM (AUC: 0.640; 95% CI: 0.572–0.707; P < 0.001 and AUC: 0.611; 95% CI: 0.544–0.679; P = 0.002, respectively). CK19 and MMP-2 expression were independent prognostic factors for disease-free survival (P = 0.031 and P = 0.012, respectively) and overall survival (P = 0.013 and P = 0.018, respectively) in HCC patients with LNM. CK19 expression (P < 0.001), MMP-2 expression (P = 0.006), and UICC T stage (P < 0.001) were independent risk factors for developing LNM in HCC. These findings show that CK19 and MMP-2 expression may be beneficial in predicting HCC LNM and survival.     

Potential signal pathway of all-trans retinoic acid for MMP-2 and MMP-9 expression in injury podocyte induced by adriamycin

Abstract

All-trans-retinoic acid (ATRA) can regulate some specific genes expression in various tissue and cells via nuclear retinoic acid receptors (RARs), including three subtypes: retinoic acid receptor-alpha (RAR-α), retinoic acid receptor-beta (RAR-β) and retinoic acid receptor-gamma (RAR-γ). Podocyte injury plays a pivotal role in the progression of glomerulosclerosis (GS). This study was performed to study the potential signal pathway of ATRA in the expression of matrix metalloproteinases-2 (MMP-2) and matrix metalloproteinases-9 (MMP-9) in injury podocyte. Cells were divided into three groups: group of negative control (NC), group of injury podocyte induced by adriamycin (ADR) (AI) and group of ADR inducing podocyte injury model treated with ATRA (AA). The cells morphology changes were detected using microscope and scanning electron microscopy. MMP-2 and MMP-9 enzymic activity was detected using the gelatin zymography method. Protein and mRNA expressions of MMP-2, MMP-9, RAR-α, RAR-β and RAR-γ were measured by western-blot and real-time RT-PCR. Enzymatic activity of MMP-2 and MMP-9 in group AA was significantly enhanced compared to AI group after ATRA-treated 24?h (p?<?0.05). The protein and mRNA expressions of MMP-2/MMP-9 in group AA were significantly increased than those in group AI at both 12 and 24?h time points (p?<?0.05). Compared to group AI, RAR-α and RAR-γ protein/mRNA expressions of group AA were significantly increased at both 12 and 24?h time points (p?<?0.05). There was no difference for the expression of RAR-β between group AI and group AA (p?>?0.05). RAR-α protein level was positively correlated with MMP-2 or MMP-9 protein expression (p?<?0.05), and RAR-γ protein level was also positively correlated with MMP-2 or MMP-9 protein expression (p?<?0.05). In conclusion, ATRA may increase expression of MMP-2 and MMP-9 by the potential signal pathway of RAR-α and RAR-γ in injury podocyte induced by adriamycin, but not RAR-β.     

Epigenetic silencing of DACH1 induces the invasion and metastasis of gastric cancer by activating TGF‐β signalling

Gastric cancer (GC) is the fourth most common malignancy in males and the fifth most common malignancy in females worldwide. DACH1 is frequently methylated in hepatic and colorectal cancer. To further understand the regulation and mechanism of DACH1 in GC, eight GC cell lines, eight cases of normal gastric mucosa, 98 cases of primary GC and 50 cases of adjacent non‐tumour tissues were examined. Methylation‐specific PCR, western blot, transwell assay and xenograft mice were used in this study. Loss of DACH1 expression correlated with promoter region methylation in GC cells, and re‐expression was induced by 5‐Aza‐2′‐deoxyazacytidine. DACH1 is methylated in 63.3% (62/98) of primary GC and 38% (19/50) of adjacent non‐tumour tissues, while no methylation was found in normal gastric mucosa. Methylation of DACH1 correlated with reduced expression of DACH1 (P < 0.01), late tumour stage (stage III/IV) (P < 0.01) and lymph node metastasis (P < 0.05). DACH1 expression inhibited epithelial–mesenchymal transition and metastasis by inhibiting transforming growth factor (TGF)‐β signalling and suppressed GC cell proliferation through inducing G2/M phase arrest. The tumour size is smaller in DACH1‐expressed BGC823 cell xenograft mice than in unexpressed group (P < 0.01). Restoration of DACH1 expression also sensitized GC cells to docetaxel. These studies suggest that DACH1 is frequently methylated in human GC and expression of DACH1 was controlled by promoter region methylation. DACH1 suppresses GC proliferation, invasion and metastasis by inhibiting TGF‐β signalling pathways both in vitro and in vivo. Epigenetic silencing DACH1 may induce GC cells' resistance to docetaxel.     

乳腺导管原位癌和浸润性导管癌中MMP-7、VEGF及E-cad的表达及临床意义

目的:探讨乳腺导管原位癌(DCIS)和浸润性导管癌(IDC)中基质金属蛋白酶-7(MMP-7)、血管内皮生长因子(VEGF)及钙黏附素E(E-cad)的表达及临床意义。方法:选取2012年1月-2017年8月期间鄂东医疗集团黄石市中心医院乳甲外科的DCIS石蜡包埋标本(DCIS组)59例,IDC石蜡包埋标本(IDC组)32例,另选取同时期正常乳腺组织标本20例为对照组,检测各组MMP-7、VEGF及E-cad的表达情况,并分析MMP-7、VEGF及E-cad的阳性表达率与DCIS、IDC患者临床病理特征的关系,采用Pearson相关性分析MMP-7、VEGF与E-cad之间的相关性。结果:DCIS组、IDC组的MMP-7、VEGF阳性表达率高于对照组,E-cad的强阳性表达率低于对照组(P0.05),DCIS组与IDC组之间的MMP-7、VEGF、E-cad阳性表达率比较差异无统计学意义(P0.05)。MMP-7、VEGF及E-cad的阳性表达率均与患者的年龄、肿瘤大小无关(P0.05),临床分期为Ⅱ-Ⅲ期、中/低分化程度、有淋巴结转移患者的MMP-7、VEGF的阳性表达率高于临床分期为Ⅰ期、高分化程度、无淋巴结转移患者(P0.05),中/低分化程度、有淋巴结转移患者的E-cad的阳性表达率低于高分化程度、无淋巴结转移患者(P0.05)。经Pearson相关性分析显示,MMP-7与VEGF存在正相关关系(r=0.362,P=0.038),MMP-7、VEGF均与E-cad无显著相关性(r=0.071、0.024,P=0.057、0.089)。结论:DCIS和IDC中MMP-7、VEGF表达较高,E-cad表达较低,且与患者临床分期、分化程度、淋巴结转移有关,临床上可以通过检查MMP-7、VEGF、E-cad的表达来评估乳腺癌的发生及发展。     

肺癌患者血清中VEGF,TIMP-1和MMP-9水平变化及临床意义

目的:研究肺癌患者血清中血管内皮生长因子(VEGF)、组织金属蛋白酶抑制剂1(TIMP-1)、基质金属蛋白酶9(MMP-9)水平变化及临床意义。方法:选取2014年3月至2016年3月来我院治疗的91例肺癌患者为病例组,同期选取40例健康者为对照组,酶联免疫吸附测定法(ELISA)测定两组血清VEGF、TIMP-1、MMP-9水平,分析肺癌患者上述指标与病理特征的关系,并采用spearman检验分析相关性。结果:病例组血清VEGF、TIMP-1、MMP-9水平均高于对照组,差异均具有统计学意义(P0.05)。肺癌患者血清VEGF、TIMP-1、MMP-9水平均与肿瘤体积大小、TNM分期、淋巴结转移、远处转移有关(P0.05)。肺癌患者血清MMP-9与TIMP-1正相关(r=0.337,P0.05)、血清MMP-9与VEGF正相关(r=0.312,P0.05)、血清TIMP-1与VEGF正相关(r=0.316,P0.05)。结论:血清VEGF、TIMP-1、MMP-9相互作用、协同参与肺癌的发生及侵袭转移,可作为肺癌诊断及预后评估的生物学标志物。     

Matrix metalloproteinas-9 functional promoter polymorphism 1562C>T increased risk of early-onset coronary artery disease

The Matrix metalloproteinas-9 functional promoter polymorphism 1562C>T may be considered an important genetic determinant of early-onset coronary artery disease (ECAD). In this study, association between MMP-9 1562C>T allele with plasma MMP-9 activity, homocysteine and lipid–lipoproteins level and ECAD in Iranian subjects was investigated. This case–control study consisted of 53 ECAD patients (age < 55 years) and unrelated late-onsets CAD (age > 70 years) who angiographically had at least 50% stenosis. MMP-9 1562C>T polymorphism was detected by PCRRFLP, plasma MMP-9 activity, serum lipid and homocysteine levels were determined by gelatin gel zymography, enzyme assay and by HPLC, respectively. The presence of MMP-9 1562C>T allele was found to be associated with ECAD (OR = 3.2, P = 0.001). The ECAD patients with MMP-9 1562C>T allele had higher MMP-9 activity (P = 0.001), LDL-C (P = 0.045), TC (P = 0.02) and homocysteine (P = 0.01) levels than the LCAD subjects. MMP-9 1562C>T allele is a risk factor for ECAD. The carriers of this allele have high levels of MMP-9 activity, LDL-C, TC and homocysteine (P = 0.01), thus, are more likely to develop myocardial infarction and CAD at young age (less than 55 years).     

Metalloproteinase’s Activity and Oxidative Stress in Mild Cognitive Impairment and Alzheimer’s Disease

Matrix metalloproteinases (MMPs) and oxidative stress have been implicated in neurological diseases such as Alzheimer’s disease (AD). Plasma MMP-2 and MMP-9 activities were assessed in Mild Cognitive Impairment (MCI) and AD subjects compared with aged-matched controls, and subsequently analysed in relation to oxidative stress markers. Both MMP-2 and MMP-9 showed no significant changes versus control subjects. Plasma glutathione peroxidase Se-dependent (GPx-Se) activity and malondialdehyde (MDA) levels were higher in AD than in controls (P < 0.05), suggesting a role for GPx-Se in controlling oxidative stress in AD. Negative correlations were observed between MMPs and MDA in AD and MCI patients (P < 0.05). In conclusion, oxidative stress events did not include activation of MMPs and this similar pattern in AD and MCI suggests that both are biochemically equivalent.     

幽门螺杆菌感染性胃癌组织中cyclinD1、MMP-9的表达及其临床意义

目的:探讨幽门螺杆菌(HP)感染性胃癌组织中细胞周期蛋白D1(cyclinD1)、基质金属蛋白酶-9(MMP-9)的表达及其临床意义。方法:选取2016年12月到2018年6月期间在兰州大学第一医院接受治疗的胃癌患者80例,收集其手术切除的病理组织。采用C-14呼气试验和改良Giemsa染色检测患者HP感染的情况,采用免疫组化法检测胃癌组织中cyclinD1、MMP-9表达情况。分析HP感染、cyclinD1、MMP-9表达与胃癌患者临床病理特征的关系,并分析胃癌患者HP感染与cyclinD1、MMP-9表达的相关性。结果:80例胃癌患者HP感染阳性56例(70.00%),阴性24例(30.00%)。有淋巴结转移、浸润深度为T3+T4的胃癌患者的HP感染阳性率高于无淋巴结转移、浸润深度为T1+T2的胃癌患者(P0.05)。80例胃癌患者cyclinD1阳性表达45例(56.25%),阴性表达35例(43.75%),MMP-9阳性表达65例(81.25%),阴性表达15例(18.75%),TNM临床分期为III+IV期、分化程度为低分化、有淋巴结转移、浸润深度为T3+T4的胃癌患者的cyclinD1、MMP-9阳性表达率明显高于TNM临床分期为I+II期、分化程度为中高分化、无淋巴结转移、浸润深度为T1+T2的胃癌患者(P0.05)。HP感染阳性患者的cyclinD1阳性表达率和MMP-9阳性表达率均明显高于HP感染阴性患者(P0.05)。Pearson相关分析显示,胃癌患者HP感染与cyclinD1、MMP-9表达均呈正相关(P0.05)。结论:胃癌患者的HP感染情况与淋巴结转移、浸润深度有关,cyclinD1和MMP-9的表达与TNM临床分期、分化程度、淋巴结转移、浸润深度有关,且胃癌患者HP感染与cyclinD1、MMP-9表达均呈正相关。     

The expression of PCNA, c-erbB-2, p53, ER and PR as well as atypical hyperplasia in tissues nearby the breast cancer

To examine the proper margin for breast conservative surgery in Chinese women population. 40 breast cancer specimens were collected and each sample was dissected into several groups: primary tumor group, 1 cm paracarcinoma, 2 cm paracarcinoma, 3 cm paracarcinoma and excessive 3 cm paracarcinoma groups. The immunohistochemistry staining was performed to measure the expression levels of proliferating cell nuclear antigen (PCNA), c-erbB-2, p53, estrogen receptor (ER) and progesterone receptor (PR). The gene expressions of PCNA, c-erbB-2, and p53 gradually decreased with the increased distance from primary tumor (P < 0.05). The 1 and 2 cm paracarcinoma group (no differences between the two, P > 0.05) showed higher risk factors (c-erbB-2, p53) than the 3 cm and excessive 3 cm paracarcinoma groups (P < 0.05). The expression of PCNA, ER, and PR showed no correlation with cancer progression (P > 0.05). Beyond the paracarcinoma 2 cm distance, the tissues showed significant decreases in tumor gene expression, which could represent the appropriate region for breast conservative surgery.     

依达拉奉联合疏血通对急性脑梗死患者的临床疗效及机制研究

目的:探讨依达拉奉联合疏血通对急性脑梗死患者的临床疗效及其可能作用机制。方法:收集我院治疗的80例急性脑梗死患者,随机分为实验组和对照组,每组40例。对照组患者给予依达拉奉注射液30 mg+生理盐水100 mg静脉滴注,2次/d;实验组患者在对照组基础上给予疏血通注射液6 g+生理盐水250 m L静脉滴注,2次/d,14 d为1个疗程。治疗后,对两组患者的血清血清基质金属蛋白酶-9(MMP-9),血管内皮生长因子(VEGF)、NIHSS以及临床疗效进行检测并比较。结果:与治疗前相比,两组患者治疗后血清MMP-9、VEGF水平以及NIHSS评分均显著下降(P0.05);与对照组相比,实验组患者治疗后血清MMP-9、VEGF水平以及NIHSS评分均较低(P0.05),且治疗总有效率较高(P0.05)。结论:依达拉奉联合疏血通能够有效提高急性脑梗死患者的临床疗效,改善神经功能缺损,这可能与其降低患者血清基质金属蛋白酶-9(MMP-9)及血管内皮生长因子(VEGF)水平有关。     

直肠系膜切除术对直肠癌根治术后局部复发患者MMPs、CEA、CA199及生存率的影响

目的:探讨直肠系膜切除术对直肠癌根治术后局部复发患者血清基质金属蛋白酶、肿瘤标志物(CEA、CA199)及生存率的影响。方法:收集直肠原发癌位于直肠中下段的病例,行直肠癌根治术复发再入院患者48例(均为本院2010年4月-2014年3月手术后的病例),按照手术方式的不同分为2组,分别24例。对照组采用姑息性手术治疗,研究组采用直肠系膜切除术治疗,采用ELISA法测定血清MMP-2、MMP-9、CEA、CA199水平,记录所有患者术后并发症状况,术后进行随访时间为3年,比较两组1年、3年的生存率状况。结果:对照组在手术时间、出血量、住院时间上高于研究组,(P0.05);对照组在肛门排气时间上低于研究组,(P0.05);与治疗前比较,两组患者治疗2周后MMP-2、MMP-9表达水平降低,治疗2周后血清CEA、CA199表达水平降低(P0.05);与对照组比较,研究组患者治疗2周后MMP-2、MMP-9表达水平较低,治疗2周后血清CEA、CA199表达水平较低(P0.05);两组患者治疗期间并发症无差异(P0.05);两组间术后1年生存率,无差异(P0.05);研究组术后3年生存率(66.67%)高于对照组(37.50%),(P0.05)。结论:直肠系膜切除术可提高直肠癌根治术后局部复发患者的长期生存率,降低血清MMP-2、MMP-9、CEA、CA199水平,安全性高,值得广泛推广。     

Study on immune regulation in Hyriopsis cumingii Lea: Effect of pearl-nucleus insertion in the visceral mass on immune factors present in the hemolymph

This study was aimed at elucidating the mechanism of immune responses in fresh water mussels during pearl culture. Alpha-2 macroglobulin gene (α₂M) expression, acid phosphatase (ACP) and superoxide dismutase (SOD) activities, and hemocyte counts were evaluated after inserting a pearl nucleus into the visceral mass of Hyriopsis cumingii Lea (H. Lea). We selected 60 H. Leas and randomly assigned them to 2 groups (each group contained 3 replicates of 10 individuals), and individuals among one group were treated by inserting pearl nucleus into the visceral mass. Real-time quantitative polymerase chain reaction (Q-PCR) was used to evaluate α₂M gene expression, and the activities of ACP and SOD in hemocytes and serum were determined after 1, 2, 3, 5, and 10 days after nucleus insertion. Hemocyte morphologies and numbers on the 5th day after insertion were studied using phase-contrast microscope (PCM), optical microscope and flow cytometry (FCM). All observations suggested that the insertion of the pearl nucleus in the visceral mass had a significant effect on α₂M gene expression, ACP and SOD activities, and hemocyte characteristics. The α₂M gene expression was sharply up-regulated on the 3rd day after nucleus insertion, and it was significantly higher in the test groups on the 3rd, 5th, and 10th days than those in the control groups (P < 0.05). On the 1st to 3rd after treatment, ACP activity in the test group was higher than that in the control group (P < 0.05). SOD activity in the serum was remarkably higher in the test groups than in the control group, and exhibited significant differences on the 3rd, 5th, and 10th days (P < 0.05). However, the SOD activity in hemocytes was lower in the test group than in the control group, and it exhibited significant differences on all days, except on the 3rd day (P < 0.05). The hemocytes were divisible into 2 types: granulocytes (GR) and hyalinocytes (HY). The hemocyte morphology, protuberances, vesicle-like bodies, and density increased significantly (P < 0.05), and the number of GR increased, while that of HY decreased after nucleus insertion. These results indicated that the insertion of pearl nucleus enhanced the immune response in H. Lea.     

MMP-2、Fhit、RECK、VEGF在喉癌组织中的表达及相关性分析

目的:探讨基质金属蛋白酶-2(MMP-2)、脆性组氨酸三联体基因(Fhit)、逆转录诱导蛋白基因(RECK)、血管内皮细胞生长因子(VEGF)在喉癌组织中的表达及其相关性。方法:选取2011年1月到2016年12月在陕西省人民医院接受治疗的喉癌患者80例,收集其手术中切除的喉癌组织和癌旁组织,另收集40例喉癌组织切除外缘的正常喉粘膜组织。比较喉癌组织、癌旁组织、正常喉粘膜组织中MMP-2、Fhit、RECK、VEGF的表达,分析喉癌组织中MMP-2、Fhit、RECK、VEGF的表达与临床病理特征的关系,并分析四个指标的相关性。结果:喉癌组织中MMP-2、VEGF表达明显高于癌旁组织和正常喉粘膜组织,Fhit、RECK表达明显低于癌旁组织和正常喉粘膜组织(P0.05)。喉癌组织中MMP-2的表达与淋巴结转移、临床分期、分化程度有关(P0.05);Fhit、RECK的表达与淋巴结转移、临床分期有关(P0.05);VEGF的表达与淋巴结转移有关(P0.05)。喉癌组织中MMP-2的表达水平与Fhit、RECK呈负相关(P0.05),与VEGF呈正相关(P0.05);Fhit与RECK呈正相关(P0.05),与VEGF呈负相关(P0.05);RECK与VEGF呈负相关(P0.05)。结论:在喉癌组织中MMP-2、Fhit、RECK、VEGF均存在异常表达;其相互影响,可能共同参与了喉癌的发生、发展。     

Dietary Taurine Supplementation Prevents Glial Alterations in Retina of Diabetic Rats

The preventive effect of dietary taurine supplementation on glial alterations in retina of streptozotocin-induced diabetic rats was examined in this study. Blood glucose content, content of taurine, glutamate and <gamma>-amino butyric acid (GABA) and expression of glial fibrillary acid protein (GFAP), vascular endothelial growth factor (VEGF), glutamate transporter (GLAST), glutamine synthetase (GS) and glutamate decarboxylase (GAD) in retina were determined in diabetic rats fed without or with 5% taurine in a controlled trial lasting 12 weeks, with normal rats fed without or with 5% taurine served as controls. Dietary taurine supplementation could not lower glucose concentration in blood (P > 0.05), but caused an elevation of taurine content and a decline in levels of glutamate and GABA in retina of diabetic rats (P < 0.05). The content of GABA in normal control group was not altered by taurine supplementation. With supplementation of taurine in diet, lower expression of GFAP and VEGF while higher expression of GLAST, GS and GAD in retina of diabetic rats were determinated by RT-PCR, Western-blotting and immunofluorescence (P < 0.05). GFAP, VEGF, GLAST, GS and GAD expressions in normal controls were not altered by taurine treatment. This may have prospective implications of using taurine to treat complications in diabetic retinopathy.