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1.
The spatial distribution and seasonal variation in the concentration and carbon isotopic composition of dissolved methane in a river–lake ecosystem were studied in Lake Biwa, Japan, and its tributary rivers. Methane concentrations in all subsystems examined were supersaturated with respect to the atmosphere. The epilimnion showed higher concentrations of dissolved methane than the hypolimnion in the pelagic zone. Peak methane concentrations were observed at the thermocline. The largest amount of methane in the pelagic water column was recorded at the end of a stagnant period, at which the bottom water of the sublittoral zone (30m in depth) exhibited increased methane concentration. Transect observation of dissolved methane revealed three methane peaks at different water depths in the lake, and river water and the sediments in littoral and sublittoral zones were suggested to be the corresponding sources. Water at the river mouth was replete with dissolved oxygen but also contained a high concentration of methane. The present results suggest that river water and littoral sediment are potential sources of dissolved methane in Lake Biwa, and other sources, such as internal waves, are responsible for increased methane in the pelagic zone at the end of stagnant periods. Carbon stable isotope analysis indicated that there were different sources of dissolved methane, although it was difficult to identify the origins due to high variation of the isotopic composition of methane from different sources. 相似文献
2.
As a part of a core project of IGBP (International Geosphere-Biosphere Programme), distribution, production, oxidation and transport processes of methane in bottom sediments and lake water in a mesotrophic lake (Lake Biwa) have been studied with special reference to the spatial heterogeneity of each process. In this study, we attempted to synthesize previously reported results with newly obtained ones to depict the methane dynamics in the entire lake. The pelagic water column exhibited subsurface maxima of dissolved methane during a stratified period. Transect observation at the littoral zone suggested that horizontal transportation may be a reason for the high methane concentration in epilimnion and thermocline at the offshore area. Tributary rivers and littoral sediments were suggested to be the source. Observations also showed that the internal wave caused resuspension of the bottom sediment and release of methane from the sediment into the lake water. The impact of the internal waves was pronounced in the late stage of a stratified period. The littoral sediment showed much higher methanogenic activity than the profundal sediments, and the bottom water of the littoral sediments had little methanotrophic activity. In the profundal sediment, most of the methane that diffused up from the deeper part was oxidized when it passed through the oxic layer. Active methane oxidation was also observed in the hypolimnetic water, while the lake water in the epilimnion and thermocline showed very low methane oxidation, probably due to the inhibitory effect of light. These results mean a longer residence time for methane in the epilimnion than in the hypolimnion. Horizontal inflow of dissolved methane from the river and/or littoral sediment, together with the longer residence time in the surface water, may cause the subsurface maxima, which have also been observed in other lakes and in the ocean. 相似文献
3.
N. V. Pimenov M. O. Ul’yanova T. A. Kanapatskii V. V. Sivkov M. V. Ivanov 《Microbiology》2008,77(5):579-586
Comprehensive microbiological and biogeochemical investigation of a pockmark within one of the sites of gas-saturated sediments in the Gdansk depression, Baltic Sea was carried out during the 87th voyage of the Professor Shtokman research vessel. Methane content in the near-bottom water and in the underlying sediments indicates stable methane flow from the sediment into the water. In the 10-m water layer above the pockmark, apart from methane anomalies, elevated numbers of microorganisms and enhanced rates of dark CO2 fixation (up to 1.15 µmol C/(l day)) and methane oxidation (up to 2.14 nmol CH4/(l day)) were revealed. Lightened isotopic composition of suspended organic matter also indicates high activity of the near-bottom microbial community. Compared to the background stations, methane content in pockmark sediments increased sharply from the surface to 40–60 ml/dm3 in the 20–30 cm horizon. High rates of bacterial sulfate reduction (SR) were detected throughout the core (0–40 cm); the maximum of 74 µmol S/(dm3 day) was located in subsurface horizons (15–20 cm). The highest rates of anaerobic methane oxidation (AMO), up to 80 µmol/dm3 day), were detected in the same horizon. Good coincidence of the AMO and SR profiles with stoichiometry close to 1: 1 is evidence in favor of a close relation between these processes performed by a consortium of methanotrophic archaea and sulfate-reducing bacteria. Methane isotopic composition in subsurface sediments of the pockmark (from ?53.0 to ?56.5‰) does not rule out the presence of methane other than the biogenic methane from the deep horizons of the sedimentary cover. 相似文献
4.
Thiols are important antioxidants that can modulate the bioavailability and biogeochemistry of many soft metals, although their detection remains challenging in both their reduced (R–S) and oxidized (R–S–S–R) forms. Here, a modified biochemical method was applied to determine the levels of dissolved and particulate thiols in Lake Biwa water and extracted Lake Biwa fulvic acids obtained at various depths. This method involves the use of the reducing agent tris(2-carboxyethyl)phosphine and the fluorescent label 7-fluorobenzofurazan-4-sulfonic acid ammonium salt (SBD-F), followed by solid-phase extraction and HPLC with fluorescence detection. Dissolved cysteine (Cys) (2.0–6.0 nM), glutathione (GSH) (2.8–5.1 nM), and N-acetyl-l-cysteine (NAC) (1.6–4.2 nM) were detected throughout the water column but were broadly consistent at depths of 5–20 m. In contrast, abundant levels of particulate cysteine (1.3–3.5?×?102 nM) and glutathione (1.6–3.1?×?102 nM) were detected down to depths of 15 m. The particulate cysteine and glutathione were significantly covariant, and the ratios between them reflected the differences in the plankton community composition and availability of these compounds. This work also studied the concentrations of Cys, GSH and NAC in Lake Biwa fulvic acids (LBFAs) for the first time (at 0 m: cysteine, 0.8 nM; glutathione, 1.6 nM; NAC, 2.5 nM; at 10 m: cysteine, 1.4 nM; glutathione, 0.6 nM; NAC, 1.6 nM). The nanomolar to sub-nanomolar concentrations of the particulate and dissolved Cys, GSH and NAC in the lake indicates that these are an important class of ligands for chalcophile metals and may influence the distribution of plankton communities from the epilimnion to the hypolimnion of the lake. 相似文献
5.
N. V. Pimenov V. N. Egorov T. A. Kanapatskii T. V. Malakhova Yu. G. Artemov P. A. Sigalevich L. V. Malakhova 《Microbiology》2013,82(5):618-627
The rates of microbial processes of sulfate reduction and of the methane cycle were measured in the bottom sediments of the Sevastopol basin, where seeps of gaseous methane have been previously found. Typically for marine environments, sulfate reduction played the major role in the terminal phase of decomposition of organic matter (OM) in reduced sediments of this area. The rate of this process depended on the amount of available OM. The rate of methanogenesis in the sediments increased with depth, peaking in the subsurface horizons, where decreased sulfate concentration was detected in the pore water. The highest rates of sulfate-dependent anaerobic methane oxidation were found close to the methane-sulfate transition zone as is typical of most investigated marine sediments. The data on the carbon isotopic composition of gaseous methane from the seeps and dissolved CH4 from the bottom sediments, as well as on the rates of microbial methanogenesis and methane oxidation indicate that the activity of the methane seeps results from accumulation of biogenic methane in the cavities of the underlying geological structures with subsequent periodic release of methane bubbles into the water column. 相似文献
6.
This modern distribution study from the southwest arm of Lake Malawi quantitatively relates variables of the lake environment to surficial assemblages of ostracodes and other paleoenvironmental indicators (molluscs, Botryococcus, fish, and charcoal) from 102 sites, across a gradient of littoral to shallow profundal conditions. The goal of this research is to use the resultant relationships to help quantify paleoecological interpretations of the fossil record from sediment cores. Site locations varied by depth (1–60 m) and adjacent shoreline environment. Thirty-three ostracode species are identified from 54 sites including four new, undescribed species of Cypridopsinae (2) and Limnocythere (2). Ostracodes are extremely abundant between 1 and 25 m water depth, but are rare to absent between 30 and 60 m. This disappearance is probably taphonomically controlled, with carbonate dissolution in the death assemblage since abundant live ostracodes have been found in the lake at greater than 30 m depth, where bottom sediments lack calcium carbonate. Constrained correspondence analysis (CCA) of ostracode species abundance suggests depth and dissolved oxygen (DO) content to be the primary environmental variables affecting their distribution. Additional CCA models using all biological indicators suggest limnologic variables correlated with depth (e.g., bottom water temperature and DO) and adjacent shoreline environment were most significant. 相似文献
7.
The rates of shrinkage at constant temperature, and growth under a temperature rise below 100°C, of bubbles entrained in wheat flour dough were analyzed and compared with those of a bubble in water. The rate of shrinkage of bubbles in flour dough was controlled by the diffusion of dissolved air from the surface of bubbles to the bulk of flour dough. The apparent diffusion coefficient of the dissolved air in wheat flour dough with the water fraction of 0.49 calculated from the shrinkage of bubbles, was (3.2 ± 1.5) × 101?1 m2/sec (19°C), and (6.4 ± 2.0) × 10?11 m2/sec (42°C). However, the growth behavior of bubbles in flour dough under a temperature rise was very different from that predicted from the diffusion theory. The critical radius of bubbles to grow was larger than that estimated from the diffusion theory. The mechanism of growth of bubbles in wheat flour dough, which was different from that of a bubble in water, is a subject that needs to be clarified. 相似文献
8.
Lake restoration with aluminium (Al) has been widely used in shallow lakes, but it is unknown how ageing of the Al floc affect resuspension behaviour, sediment stability and entrainment of Al and phosphorus (P). High primary production in shallow lakes can lead to high pH in the water column with a potential release of Al and P during resuspension events. A laboratory resuspension experiment at defined hydrodynamic conditions using a calibrated erosion chamber was performed with intact sediment cores (with intact vs. disturbed biofilm on the surface) from previously Al-treated shallow Lake Möllen, NE Germany. Newly applied Al reduced surface sediment stability, but ageing led to the same stability as untreated sediment within 2 months with an intact biofilm and within 4 months with a disturbed biofilm. Dissolved Al increased markedly at resuspension after 2 days and 2 weeks of ageing and with elevated pH (9–11) in the water. The Al floc were redissolved and dissolved Al increased with elevated pH and shear velocity. Dissolved P concentration was constantly low due to excess binding capacity of the Al floc. In conclusion, Al application to shallow lakes prone to resuspension and with a high production must be done in periods with less resuspension risk to allow 2–4 months time for floc stabilization. Otherwise, possible resuspension and high(er) pH may lead to elevated concentrations of dissolved Al in the water column. 相似文献
9.
A 2-year study was designed to investigate the spatiotemporal distribution of sulphide in the water column of a saline lake, Lake Hiruga in Fukui, Japan. Water samples were collected in Lake Hiruga periodically between April 2014 and March 2016 at appropriate depths with simultaneous in situ measurements of physicochemical parameters such as temperature, salinity and dissolved oxygen (DO) concentration. Sulphide started accumulating in the hypolimnion in September 2014, whilst that did in June 2015. The sulphide concentrations increased toward the end of December in both 2014 and 2015. Then the sulphide disappeared from the hypolimnion in April 2015 and February 2016. The difference in starting season of the sulphide accumulation was closely connected with DO concentrations in the hypolimnion in circulation periods of winter season. The DO concentrations were regulated by water column stability before thermal stratification periods. Our analysis of the sulphide distribution for the 2 years of the study suggested that accumulation of sulphide in the hypolimnion during stratified periods appears to be associated with strength of water column stability in the winter-spring seasons before stagnant periods in Lake Hiruga. 相似文献
10.
J. Elizabeth Corbett Malak M. Tfaily David J. Burdige William T. Cooper Paul H. Glaser Jeffrey P. Chanton 《Biogeochemistry》2013,114(1-3):327-340
Although methanogenic pathways generally produce equimolar amounts of carbon dioxide and methane, CO2 concentrations are often reported to be higher than CH4 concentrations in both field and laboratory incubation studies of peat decomposition. In field settings, higher pore water concentrations of CO2 may result from the loss of methane by: (1) ebullition due to the low solubility of methane in pore water and (2) vascular-plant transport. Higher CO2 concentrations may also be caused by: (1) production of additional CO2 by high-molecular weight (HMW) organic matter (OM) fermentation and/or (2) respiration from non-methanogenic pathways. In this study of a peatland where advection and transverse dispersion were the dominant pore water solute transport mechanisms, an isotope-mass balance approach was used to determine the proportions of CO2 formed from non-fractionating OM respiration and HMW fermentation relative to CO2 production from methanogenesis. This approach also allowed us to estimate the loss of CH4 from the belowground system. The pathways of CO2 production varied with depth and surface vegetation type. In a Carex-dominated fen, methane production initially produced 40 % of the total CO2 and then increased to 90–100 % with increasing depth. In a Sphagnum-dominated bog, methanogenesis resulted in 60 % of total CO2 production which increased to 100 % at depth. Both bogs and fens showed 85–100 % of methane loss from pore waters. Our results indicate that the isotopic composition of dissolved CO2 is a powerful indicator to allow partitioning of the processes affecting peat remineralization and methane production. 相似文献
11.
Brandi Kiel Reese Heath J. Mills Scot E. Dowd John W. Morse 《Geomicrobiology journal》2013,30(2):160-172
Multiple environmental mechanisms have been proposed to control bottom water hypoxia (<2 mg O2 L?1) in the northern Gulf of Mexico Louisiana shelf. Near-bottom hypoxia has been attributed to a direct consumption of oxygen through benthic microbial respiration and a secondary chemical reaction between oxygen and reduced metabolites (i.e. ferrous iron and total sulfide) from these populations. No studies to date have examined the metabolically active microbial community structure in conjunction with the geochemical profile in these sediments. Temporal and spatial differences in dissolved and solid phase geochemistry were investigated in the upper 20 cm of the sediment column. Pyrosequencing of reverse transcribed small subunit (SSU) ribosomal ribonucleic acid (rRNA) was used to determine population distribution. Results indicated that populations shallower than 10 cm below surface were temporally variable yet uniform between sites, while below this depth, populations were more site-specific. This suggests a potential interaction between the water column and the benthic microbial population limited to a shallow depth. The presence of dissolved reduced iron in the upper sediment column was indicative of low oxygen concentration, yet sulfide was at or below detection limits. Putative sulfate and iron reducing and oxidizing populations were metabolically active at similar depths suggesting potential recycling of products. Results from this study indicate low carbon concentrations in the shallow sediments limit general metabolic activity, reducing the potential for microbial respiration. Supplemental materials are available for this article. Go to the publisher's online edition of Geomicrobiology Journal to view the supplemental file. 相似文献
12.
Prior to an analysis of the shrinkage and growth of air bubbles entrained in wheat flour dough, the shrinkage and growth under a temperature rise of a small bubble in water was analysed for comparison. The rates of shrinkage and growth of the bubble were respectively controlled by the diffusion of under- and over-saturated dissolved air from and into the bubble. The diffusion coefficient of the dissolved air in water calculated from the shrinkage of the bubble was 2.10 × 10_9m2/sec (17°C), which agrees with the literature value. On the other hand, at below 100°C, the effects on the bubble growth of the expansion of air due to the temperature rise and the increase in the saturation vapor pressure of water were negligibly small. The accompanying air entrained in flour particles suspended in water was much more stable than a free bubble in water. However, the growth under a temperature-rise of a bubble evolved from wheat flour particles was the same as the growth of a bubble in water, if many bubbles did not coexist. 相似文献
13.
Stable isotopes track biogeochemical processes under seasonal ice cover in a shallow,productive lake
Christopher H. Gammons William Henne Simon R. Poulson Stephen R. Parker Tyler B. Johnston John E. Dore Eric S. Boyd 《Biogeochemistry》2014,120(1-3):359-379
Biogeochemical dynamics under seasonal ice cover were investigated in the shallow (<10 m) water column of highly productive Georgetown Lake, western Montana, USA. This high altitude (1,800 m) reservoir is well-mixed in summer, but becomes strongly stratified under ice cover (mid-November–mid-May). A rapid drop in dissolved oxygen (DO) concentration and rise in dissolved inorganic carbon (DIC) concentration was observed after the onset of ice, with a corresponding increase in δ18O-DO and decrease in δ13C-DIC, likely caused by respiration (R) of organic carbon. Photosynthesis/respiration ratios (P/R) estimated from simultaneous measurement of DO and δ18O-DO were near unity prior to ice formation but then systematically decreased with time and depth in the lake under ice cover. P/R in the water column was higher at a shallower monitoring site compared to a deeper site near the dam outlet, which may have been important for over-winter survival of salmonids. By March, the bottom 3 m of the water column at both sites was anoxic, with the bottom 1 m being euxinic. Elevated concentrations of dissolved sulfide, ammonium, phosphate, Fe2+, and Mn2+ in deep water suggest coupling of organic carbon degradation with reduction of a number of electron acceptors (e.g., Fe3+, \({\text{NO}}_{3}^{ - } ,\;{\text{SO}}_{4}^{2 - }\) ). The concentrations and δ34S values of H2S in the deep water and \({\text{SO}}_{4}^{2 - }\) in the shallow water were similar, indicating near-complete reduction of sulfate in the euxinic zone. Late in the winter, an influx of isotopically heavy DIC was noted in the deep water coincident with a buildup of dissolved CH4 to concentrations >1 mM. These trends are attributed to acetoclastic methanogenesis in the benthic sediments. This pool of dissolved CH4 was likely released from the lake to the atmosphere during spring ice-off and lake turnover. 相似文献
14.
J. K. Klar W. B. Homoky P. J. Statham A. J. Birchill E. L. Harris E. M. S. Woodward B. Silburn M. J. Cooper R. H. James D. P. Connelly F. Chever A. Lichtschlag C. Graves 《Biogeochemistry》2017,135(1-2):49-67
Shelf sediments underlying temperate and oxic waters of the Celtic Sea (NW European Shelf) were found to have shallow oxygen penetrations depths from late spring to late summer (2.2–5.8 mm below seafloor) with the shallowest during/after the spring-bloom (mid-April to mid-May) when the organic carbon content was highest. Sediment porewater dissolved iron (dFe, <0.15 µm) mainly (>85%) consisted of Fe(II) and gradually increased from 0.4 to 15 μM at the sediment surface to ~100–170 µM at about 6 cm depth. During the late spring this Fe(II) was found to be mainly present as soluble Fe(II) (>85% sFe, <0.02 µm). Sub-surface dFe(II) maxima were enriched in light isotopes (δ56Fe ?2.0 to ?1.5‰), which is attributed to dissimilatory iron reduction (DIR) during the bacterial decomposition of organic matter. As porewater Fe(II) was oxidised to insoluble Fe(III) in the surface sediment layer, residual Fe(II) was further enriched in light isotopes (down to ?3.0‰). Ferrozine-reactive Fe(II) was found in surface porewaters and in overlying core top waters, and was highest in the late spring period. Shipboard experiments showed that depletion of bottom water oxygen in late spring can lead to a substantial release of Fe(II). Reoxygenation of bottom water caused this Fe(II) to be rapidly lost from solution, but residual dFe(II) and dFe(III) remained (12 and 33 nM) after >7 h. Iron(II) oxidation experiments in core top and bottom waters also showed removal from solution but at rates up to 5-times slower than predicted from theoretical reaction kinetics. These data imply the presence of ligands capable of complexing Fe(II) and supressing oxidation. The lower oxidation rate allows more time for the diffusion of Fe(II) from the sediments into the overlying water column. Modelling indicates significant diffusive fluxes of Fe(II) (on the order of 23–31 µmol m?2 day?1) are possible during late spring when oxygen penetration depths are shallow, and pore water Fe(II) concentrations are highest. In the water column this stabilised Fe(II) will gradually be oxidised and become part of the dFe(III) pool. Thus oxic continental shelves can supply dFe to the water column, which is enhanced during a small period of the year after phytoplankton bloom events when organic matter is transferred to the seafloor. This input is based on conservative assumptions for solute exchange (diffusion-reaction), whereas (bio)physical advection and resuspension events are likely to accelerate these solute exchanges in shelf-seas. 相似文献
15.
ABSTRACT. A quantitative study of the seasonal distribution of thermotolerant (37°C and 45°C), small free-living amoebae (FLA) was conducted in Willard's Pond, a warm, monomictic lake in the Piedmont region of South Carolina. Correlation of physical and chemical parameters with the seasonal distribution was facilitated by partitioning the aquatic ecosystem into benthic, planktonic, and neustonic habitats. Population densities of FLA peaked in late summer in each habitat; however, species composition varied between habitats. Littoral sediment appeared to be the major habitat for FLA, with peaks in populations of Acanthamoeba and Naegleria in August, Hartmannella in July, and Vahlkampfia in May. Populations in profundal sediment underwent dramatic seasonal shifts, apparently in response to the seasonal chemical changes in the hypolimnion. Acanthamoeba was most prevalent in late summer, representing as much as 82% of the FLA in profundal sediment. Distribution patterns and species composition of FLA from surface water were similar to those from littoral sediment; however, a greater percentage of Naegleria was found in surface water. Numerous FLA were isolated from the neustonic community (surface film), and the number of FLA isolated in the surface film at the deep water station was found to be significantly (P > 0.05) greater than the number from subsurface (5–10 cm) samples. In the water column, FLA populations consistently were highest in the detrital layer, which persisted at a depth of 3.0–3.4 m throughout the summer period. The large percentage of Naegleria contributing to FLA in the detrital layer suggests that Naegleria amoeboflagellates sink through the layer, flagellate, and swim back up, such migrations possibly being triggered by a reduction of nutrients below the layer or by the presence of anoxic, reducing conditions in the hypolimnion. In addition, weather events were found to play a major role in the redistribution of FLA between various habitats in the aquatic ecosystem, with such changes probably due to resuspension of FLA from littoral sediment by wind action and input from the watershed via runoff. 相似文献
16.
Activity and diversity of methanotrophic bacteria at methane seeps in eastern Lake Constance sediments 总被引:1,自引:0,他引:1
The activity and community structure of aerobic methanotrophic communities were investigated at methane seeps (pockmarks) in the littoral and profundal zones of an oligotrophic freshwater lake (Lake Constance, Germany). Measurements of potential methane oxidation rates showed that sediments inside littoral pockmarks are hot spots of methane oxidation. Potential methane oxidation rates at littoral pockmark sites exceeded the rates of the surrounding sediment by 2 orders of magnitude. Terminal restriction fragment length polymorphism (T-RFLP) analysis of the pmoA gene revealed major differences in the methanotrophic community composition between littoral pockmarks and the surrounding sediments. Clone library analysis confirmed that one distinct Methylobacter-related group dominates the community at littoral pockmarks. In profundal sediments, the differences between pockmarks and surrounding sediments were found to be less pronounced. 相似文献
17.
Schubert CJ Coolen MJ Neretin LN Schippers A Abbas B Durisch-Kaiser E Wehrli B Hopmans EC Damsté JS Wakeham S Kuypers MM 《Environmental microbiology》2006,8(10):1844-1856
Inputs of CH(4) from sediments, including methane seeps on the continental margin and methane-rich mud volcanoes on the abyssal plain, make the Black Sea the world's largest surface water reservoir of dissolved methane and drive a high rate of aerobic and anaerobic oxidation of methane in the water column. Here we present the first combined organic geochemical and molecular ecology data on a water column profile of the western Black Sea. We show that aerobic methanotrophs type I are responsible for methane oxidation in the oxic water column and ANME-1- and ANME-2-related organisms for anaerobic methane oxidation. The occurrence of methanotrophs type I cells in the anoxic zone suggests that inactive cells settle to deeper waters. Molecular and biomarker results suggest that a clear distinction between the occurrence of ANME-1- and ANME-2-related lineages exists, i.e. ANME-1-related organisms are responsible for anaerobic methane oxidation below 600 m water depth, whereas ANME-2-related organisms are responsible for this process in the anoxic water column above approximately 600 m water depth. 相似文献
18.
The effect of sewage effluent on denitrification in Grasmere (English Lake District) 总被引:1,自引:0,他引:1
SUMMARY. Denitrification processes in Grasmere were studied using several techniques. Sediments were analysed for nitrate reductase activity and the accumulation of nitrogenous gases in samples incubated in the laboratory. Changes in the concentrations of inorganic nitrogen species in the water column and the release of gas bubbles into gas traps suspended above the sediment were also measured. Sewage effluent entered the lake via the main inflow and faecal bacteria were used as tracers to map the path of the river (and effluent) across the lake. The main river flow was west of the island in the centre of the lake and across the smaller of the two main basins. A preliminary study indicated that this western basin was the site of most active denitrification and this was confirmed in the following year when a seasonal survey showed that deoxygenation, nitrate reduction and ammonium accumulation was more advanced at this site. In addition, this basin was the only area where consistent discharge of nitrogen and methane from the profundal zone was observed. The nitrate reductase assay demonstrated that the sediment was the major site of denitrification, and that activity in the water column was higher in the anoxic hypolimnion than in the euphotic zone. Gas evolution from incubated sediment samples was analysed by gas liquid chromatography (GLC) and indicated that maximum denitrifying activity occurred below the mud surface in oxidized sediments. There was reasonable agreement between the enzyme assay and the GLC technique but neither predicted events in the field satisfactorily. Viable, most probable number (MPN) counts of denitrifying bacteria had similar limitations. A bimodal seasonal pattern of nitrate reductase activity was observed at four sites during thermal stratification, but this could not be explained fully. The major site of denitrification was identified and it was calculated that the nitrogen gas discharged was equivalent to c. 54% of the nitrate removed from the water column. 相似文献
19.
F. I. Leith M. H. Garnett K. J. Dinsmore M. F. Billett K. V. Heal 《Biogeochemistry》2014,119(1-3):415-433
Radiocarbon isotopes are increasingly being used to investigate the age and source of carbon released from peatlands. Here we use combined 14C and δ13C measurements to determine the isotopic composition of soil and soil decomposition products [dissolved organic carbon (DOC), CO2 and CH4] in a peatland–riparian–stream transect, to establish the isotopic signature and potential connectivity between carbon pools. Sampling was conducted during two time periods in 2012 to investigate processes under different temperature, hydrological and flux conditions. Isotopic differences existed in the peatland and riparian zone soil organic matter as a result of the riparian depositional formation. The peatland had a mean radiocarbon age of 551 ± 133 years BP, with age increasing with depth, and δ13C values consistent with C3 plant material as the primary source. In contrast the riparian zone had a much older radiocarbon age of 1,055 ± 107 years BP and showed no age/depth relationship; δ13C in the riparian zone was also consistent with C3 plant material. With the exception of DOC in September, soil decomposition products were predominately >100 %modern with 14C values consistent with derivation from organic matter fixed in the previous 5 years. Emissions of CO2 and CH4 from the soil surface were also modern. In contrast, CO2 and CH4 evaded from the stream surface was older (CH4: 310–537 years BP, CO2: 36 years BP to modern) and contained a more complex mix of sources combining soil organic matter and geogenic carbon. The results suggest considerable vertical transport of modern carbon to depth within the soil profile. The importance of modern recently fixed carbon and the differences between riparian and stream isotopic signatures suggests that the peatland (not the riparian zone) is the most important source of carbon to stream water. 相似文献
20.
The microbial segment of food webs plays a crucial role in lacustrine food-web functioning and carbon transfer, thereby influencing carbon storage and CO2 emission and uptake in freshwater environments. Variability in microbial carbon processing (autotrophic and heterotrophic production and respiration based on glucose) with depth was investigated in eutrophic, methane-rich Lake Rotsee, Switzerland. In June 2011, 13C-labelling experiments were carried out at six depth intervals in the water column under ambient light as well as dark conditions to evaluate the relative importance of (chemo)autotrophic, mixotrophic and heterotrophic production. Label incorporation rates of phospholipid-derived fatty acid (PLFA) biomarkers allowed us to differentiate between microbial producers and calculate group-specific production. We conclude that at 6 m, net primary production (NPP) rates were highest, dominated by algal photoautotrophic production. At 10 m —the base of the oxycline— a distinct low-light community was able to fix inorganic carbon, while in the hypolimnion, heterotrophic production prevailed. At 2 m depth, high label incorporation into POC could only be traced to nonspecific PLFA, which prevented definite identification, but suggests cyanobacteria as dominating organisms. There was also depth zonation in extracellular carbon release and heterotrophic bacterial growth on recently fixed carbon. Large differences were observed between concentrations and label incorporation of POC and biomarkers, with large pools of inactive biomass settling in the hypolimnion, suggesting late-/post-bloom conditions. Net primary production (115 mmol C m?2 d?1) reached highest values in the epilimnion and was higher than glucose-based production (3.3 mmol C m?2 d?1, highest rates in the hypolimnion) and respiration (5.9 mmol C m?2 d?1, highest rates in the epilimnion). Hence, eutrophic Lake Rotsee was net autotrophic during our experiments, potentially storing large amounts of carbon. 相似文献