Olfactory discrimination and transient mood change in young men and women: variation by season, mood state, and time of day

Odor performance varies by clinical state and gender, though little is known about its variation by season or time of day. Many odors, including lavender, induce transient mood changes. This study explored discrimination differences between various lavender oil blends and their effects on transient mood in the morning and evening in depressed and nondepressed adults. We also determined seasonal influences on these measures. A total of 169 subjects, 98 women and 71 men (mean age +/- SD, 19.3 +/- 1.6 y) participated, with different subjects studied at different times of the year. The Beck Depression Inventory (BDI) classified subjects as depressed (score > or =10; N= 57) or nondepressed (score <10; N= 112). In the discrimination test, subjects compared pairs of two different lavender oil blends or a control. Transient change in mood was assessed by the Profile of Mood States (POMS) after each trial of five lavender blends interspersed by three control odors. Tests were conducted in the morning (08:00-10:00 h) and evening (18:00-20:00h). In all subjects, discrimination was significantly better for some odor pairs than for others, thus demonstrating test specificity. Discrimination was better overall in the fall than winter/spring and better in depressed than nondepressed subjects for specific odor pairs. No significant gender or time-of-day differences in discriminability were detected. There were, however, significant group differences in transient mood profiles. Current depressed state affected mood response, with lavender increasing anger in depressed subjects only. In addition, depressed subjects and men, whether or not depressed, exhibited diurnal mood variation, with better mood in the evening; the former group also showed more evening energy. All subjects were more confused in the morning than evening. Season also affected transient mood; winter/spring-tested subjects reported more vigor than fall-tested subjects. In addition, summer-tested subjects showed more tension in the morning, whereas fall-tested subjects showed the opposite pattern in the evening. In all subjects, lavender increased fatigue, tension, confusion, and total mood disturbance, and it decreased vigor. The study showed that both chronobiological (seasonal and time-of-day) and clinical factors modify discrimination and mood response to odors. Brief lavender odor presentation may serve as a nonphotic method for altering mood in young depressed and nondepressed adults particularly during the fall, a time of heightened discriminability.     

The Relationship of Dream Content and Changes in Daytime Mood in Traumatized vs. Non-Traumatized Children

The study examined how the mood changes from night to morning, and how dysphoric dream contents associate with this change among children who live in traumatic environment and their controls from peaceful area. The sample consisted of 413 Palestinian boys and girls of 6–15 years of age, the mean age being 11.22 ± 2.64. The participants filled in a seven-day dream diary in which they recorded their recalled dreams every morning. First, the results, confirmed that mood change from evening to morning is a general dream function: age and gender are not related to the change. The mood chance was rather associated with what and whom the children dreamt about. Second, the hypothesis of the trauma group showing less change in dysphoric dream content and in the intensity of negative morning mood across a period of time of seven days was not confirmed. On the contrary, the results showed that both dreams incorporating dysphoric themes and negative morning mood decreased only among children living in traumatic conditions. Third, it was hypothesized that there is a stronger association between presleep negative mood and dysphoric dreams, as well as between the dysphoric dreams and negative morning mood among children living in traumatic environment than among children from peaceful area. Contrary to the hypothesis, results for the trauma group revealed a reverse association between evening mood and dream contents: the more afraid, angry and worried children felt in the evening, the more Happy recreation dreams they reported, and the happier evening mood they reported, the more Threatening stranger dreams they had. However, concurring with the hypothesis, a direct association was found between dysphoric dreams and negative morning mood in the trauma group. The more children dreamt about Threatening strangers, the more afraid, angry and worried they felt in the morning. The discussion proposes a model of the correcting or balancing dream function that is characterized by an reverse assimilation of incorporating evening mood into dreams, and by a direct accommodation of dream content into morning mood.     

Chronotype,gender, and time for sex

The study aimed at testing chronotype and gender differences in the time of day when humans feel the greatest need for sex and the time of day they actually undertake sexual activity. A Polish sample of 565 participants aged between 18 and 57 was tested. In females, regardless of chronotype, the greatest need for sex occurred between 18:00 and 24:00, but a secondary peak appeared only in morning types at 6:00–9:00. In males, the greatest need for sex occurred either in the morning or evening hours: in evening types at 9:00–12:00 and 18:00–3:00; in neither types at 6:00–9:00 and 18:00–24:00; in morning types at 6:00–12:00 and 18:00–24:00. Considering time of day when subjects were undertaking sexual activity most frequently, this appeared between 18:00 and 24:00 for all the participants, and prolonged until 3:00 at night in evening type males. Morningness preference was more strongly related to the timing of need for sex than to the timing of actual sexual activity (r?=??0.275 vs. r?=??0.174), while the timing of desire and the timing of sexual activity were positively, but moderately related (r?=?0.320).     

Morning versus evening power output and repeated-sprint ability

We investigated the effect of time-of-day on both maximal sprint power and repeated-sprint ability (RSA). Nine volunteers (22+/-4 yrs) performed a RSA test both in the morning (07:00 to 09:00 h) and evening (17:00 to 19:00 h) on different days in a random order. The RSA cycle test consisted of five, 6 sec maximal sprints interspersed by 24 sec of passive recovery. Both blood lactate concentration and heart rate were higher in the evening than morning RSA (lactate values post exercise: 13+/-3 versus 11+/-3 mmol/L(-1), p<0.05). The peak power developed during the first sprint was higher in the evening than morning (958+/-112 vs. 915+/-133 W, p<0.05), but this difference was not apparent in subsequent sprints, leading to a higher power decrement across the 5x6 sec test in the evening (11+/-2 vs. 7+/-3%, p<0.05). Both the total work during the RSA cycle test and the power developed during bouts 2 to 5 failed to be influenced by time-of-day. This suggests that the beneficial effect of time-of-day may be limited to a single expression of muscular power and fails to advantage performance during repeated sprints.     

MAXIMAL POWER,BUT NOT FATIGABILITY,IS GREATER DURING REPEATED SPRINTS PERFORMED IN THE AFTERNOON

The present study was designed to investigate if the suggested greater fatigability during repeated exercise in the afternoon, compared to the morning, represents a true time-of-day effect on fatigability or a consequence of a higher initial power. In a counterbalanced order, eight subjects performed a repeated-sprint test [10?×?(6 s of maximal cycling sprint?+?30 s of rest)] on three different occasions between: 08:00–10:00, 17:00–19:00, and 17:00-19:00?h controlled (17:00–19:00?h_cont, i.e., initial power controlled to be the same as the two first sprints of the 08:00–10:00?h trial). Power output was significantly (p?<?0.05) higher for sprints 1, 2, and 3 in the afternoon than in the morning (e.g., sprint 1: 23.3 ±1 versus 21.2 ±1 W·kg^?1), but power decrement for the 10 sprints was also higher in the afternoon. Based on the following observations, we conclude that this higher power decrement is a consequence of the higher initial power output in the afternoon. First, there was no difference in power during the final five sprints (e.g., 20.4 ±1 versus 19.7 ±1 W·kg^?1 for sprint 10 in the afternoon and morning, respectively). Second, the greater decrement in the afternoon was no longer present when participants were producing the same initial power output in the afternoon as in the morning. Third, electromyographic activity of the vastus lateralis decreased during the exercise (p?<?0.05), but without a time-of-day effect. (Author correspondence: sebastien.racinais@aspetar.com)     

Odors: implicit memory and performance effects.

In order to assess the influence of odors on human performance and implicit memory for odors, 108 subjects completed a variety of tests in weakly scented (jasmine, lavender or odorless) rooms without having been made aware of the odor. After a 30 min interval the subjects were shown slides of different surroundings, including the room they had been in, and were requested to rate how well a set of 12 odors, including a blank, would fit to these surroundings. Half of these contexts contained visual cues related to two of the presented odors (leather and coffee). After the rating of fit the subjects had to rate the odors for pleasantness, were asked to identify the odors with their correct names and to tell where and when they had last smelled these odors. One subject remembered smelling the odor (jasmine) in the room and was discarded from the analysis of the results for the rating of fit. None of the others reported recollection of the experimental odors. The results showed that in general jasmine had a negative and lavender a positive effect on test performance. If an odor-related visual cue was present in the context, the related odor was always rated highest in fit to that context. Furthermore, the subjects working in rooms with an odor subsequently assigned this odor to the visual context of that room to a significantly higher degree than subjects working in rooms with different odors. Since none of the subjects reported that they had smelled the odor in the rooms where performance testing took place, it was concluded that the memory for these odors was implicit. Further analysis showed that such memory was only found in subjects who were unable to supply the right name for the odor. The possible consequences of this latter finding for understanding the relationship between sensory (episodic) and semantic odor memory are discussed.     

Effects of inhalation of essential oils on EEG activity and sensory evaluation

The purpose of this study was to investigate EEG changes in subjects directly after inhalation of essential oils, and subsequently, to observe any effect on subjective evaluations. EEG and sensory evaluation were assessed in 13 healthy female subjects in four odor conditions. Four odor conditions (including lavender, chamomile, sandalwood and eugenol) were applied respectively for each subject in the experiment. The results were as follows. 1) Four basic factors were extracted from 22 adjective pairs by factor analysis of the sensory evaluation. The first factor was "comfortable feeling", the second "cheerful feeling", the third "natural feeling" and the fourth "feminine feeling". In the score of the first factor (comfortable feeling), the odors in order of high contribution are lavender, eugenol, chamomile and sandalwood. 2) Alpha 1 (8-10 Hz) of EEG at parietal and posterior temporal regions significantly decreased soon after the onset of inhalation of lavender oil (p < 0.01). Significant changes of alpha 1 were also observed after inhalation of eugenol or chamomile. The change after inhalation of sandalwood was not significant. These results showed that alpha 1 activity significantly decreased under odor conditions in which subjects felt comfortable, and showed no significant change under odor conditions in which subjects felt uncomfortable. These results suggest a possible correlation between alpha 1 activity and subjective evaluation.     

Chronotype regulates the neural basis of response inhibition during the daytime

Studies have elucidated the various modulatory effects of chronotype and time-of-day on task-dependent brain activity, but it is unclear how chronotype and time-of-day regulate brain activity in response inhibition tasks. To address this question, we used functional magnetic resonance imaging (fMRI) to explore the effects of chronotype and time-of-day on response inhibition in normal day-night conditions. Morning-type (MT) and evening-type (ET) participants conducted the stop-signal task in morning (08:00–12:00 hours) and evening (19:00–23:00 hours) sessions. The results showed that inhibition-related cerebral responses in the medial frontal gyrus (MFG), middle cingulate cortex (MCC), thalamus and other typical regions for the execution of response inhibition significantly decreased from morning to evening in MT participants, whereas activity in the right inferior frontal gyrus (IFG)/insula, MFG, MCC and thalamus remained stable or increased in ET participants. The chronotypical differences in homeostatic sleep pressure may explain the observed individual differences in maintaining cognition-related cortical activation. These results suggest the importance of considering chronotype and time-of-day in the design and analysis of cognitive neuroscience studies.     

Time-of-Day Effects on Anaerobic Muscular Power in a Moderately Warm Environment

This study evaluated the influence of a neutral vs. a moderately warm environment on the diurnal variation in muscular power. Twelve male subjects [27.0 (±4) years] performed two different jump tests [a squat jump (SJ) and a counter-movement jump (CMJ)] and a brief maximal sprint on cycle ergometer (CS) in four different conditions (morning/neutral, morning/moderately warm and humid, afternoon/neutral, and afternoon/moderately warm and humid). The morning experiments were conducted between 07:00 and 09:00 h, and the afternoon experiments were conducted between 17:00 and 19:00 h. The mean laboratory temperatures and humidity were 20 (±1)°C, 70 (±5)% and 29 (±1)°C, 57 (±4)% for the neutral and moderately warm and humid conditions, respectively. Rectal temperature and leg skin temperature were significantly dependent on both time-of-day and ambient temperature. An interaction effect (P < 0.05) was noted between time-of-day and ambient temperature for the power developed for the CMJ, the SJ, and half of a pedal revolution during the cycling sprint. In summary, (i) the same subjects were influenced by time-of-day differently, depending on the ambient temperature during testing; (ii) time-of-day affected muscular performance only in the neutral condition, (iii) the moderately warm and humid condition blunted the diurnal variation in muscular performance, and (iv) the effect of the ambient temperature was dependent on time-of-day.     

Effect of time of day on the offensive capability and aerobic performance in football game

The purpose of this study was to examine the time-of-day effects on the offensive capability and aerobic performance in football game in young subjects. In a randomized order, participants realized the Yo–Yo intermittent recovery test in two test sessions and a football game situations (two 15-min games), interspersed by a verbalization sequence (3 min) at 08:00 and 17:00 h on separate days. A recovery period of 24 h was permitted between two consecutive test sessions. The results revealed diurnal variations on the maximal aerobic velocity during the Yo–Yo test (MAV) and the oral temperature with higher values in the afternoon than morning (p < 0.05). Concerning offensive capability, the numbers of scored goals were significantly higher at 17:00 h in comparison with 08:00 h (p < 0.05). However, there was no significant difference between 08:00 and 17:00 h for the kicked balls (shooting parameter). In conclusion, our findings suggest that performance was improved in the evening and the parameters (shooting and Scored goals) can be used as a model to describe the offensive capacity in football game depending on the time of day.     

Influence of Menstrual Cycle Phase and Oral Contraceptive Use on the Time-of-Day Effect on Maximal Anaerobic Power

This study was designed to analyse the time-of-day effect in maximal anaerobic power, and the influence of menstrual cycle phase and oral contraceptive use on any diurnal effect. Diurnal variations in maximal cycling power were studied in 11 eumenorrheic women and 10 women using monophasic oral contraceptives. Subjects were tested at 09:00, 14:00 and 18:00 hours, assigned randomly on separate days, in the mid-follicular or pseudo-follicular phase (days 7, 8, 9) and in the mid-luteal or pseudo-luteal phase (days 19, 20, 21) of the menstrual cycle. The order of test sessions was randomly assigned. Body mass was measured before, and rectal temperature after, a standardized 15-min warm-up. Maximal cycling power (Pc) was determined by a force-velocity test. Rectal temperature significantly increased from morning (09:00) to afternoon (14:00 and 18:00) in follicular and luteal phases for eumenorrheic subjects, and in days 7–9 and days 19–21 for contraceptive users (p < 0.05). No significant interaction effects (time of day × group × cycle phase) were observed for rectal temperature. In eumenorrheic subjects, Pc increased significantly from 09:00 to afternoon during the follicular phase (P < 0.05). In contrast, no significant time-of-day effects were observed during the luteal phase in eumenorrheic subjects, and at any cycle phase in contraceptive users. Analysis of variance failed to reveal any significant interaction effects for Pc. This study suggested that the time-of-day effect on maximal anaerobic power could be damped during the luteal phase of eumenorrheic women or at any cycle phase by oral contraceptive use.     

The effect of training at the same time-of-day on the diurnal variations of technical ability and swimming performance

The aim of this study was to examine the effects of training at the same time of day on diurnal variations of technical ability and swimming performance, to provide some recommendations with regard to adjusting training hours in accord with the time of day of competitive events. Eighteen participants volunteered for this study, and these were randomly assigned to either a morning training group (MTG, who trained only between 07:00 and 08:00 h, n = 6), an evening training group (ETG, who trained only between 17:00 and 18:00 h, n = 6), or a control group (CG, did not train but participated in all tests, n = 6). Swimming performance and technical ability – (i) stroke parameters: swim velocity (V), stroke rate (SR), and stroke length (SL); and (ii) motor organization: arm stroke phases and arm coordination (Idc) – were recorded 2 weeks before and 2 weeks after an 8-week regular training period. For all participants, the morning and evening tests were scheduled at the same time of day as the morning and evening training sessions. After training, the major finding of this study was that both ETG and the CG showed significantly lower P, V, SR, phase (B), phase (C), and Idc values in the morning than in the evening. However, P, V, SR, phase (B), phase (C), and Idc of the MTG measured at 07:00 and 17:00 h did not differ. Thus, training at a specific time of day increased performance in MTG at this time and modified the diurnal variation of swim performance. This study indicates that training at a specific time of day can result in marked changes in both swimming performance and technical aspects of swimming. Furthermore, training in the morning improved morning swimming performance and its components, and the amplitude of the morning–evening difference decreased. Training in the evening improved swimming performance and its components more in the evening than the morning, and the amplitude of the morning–evening difference increased.     

Olfactory discrimination ability for aliphatic c6 alcohols as a function of presence, position, and configuration of a double bond

The ability of human subjects to distinguish between aliphatic C6 alcohols differing in presence, position, or configuration (i.e., cis-trans geometry) of a double bond was tested. In a forced-choice triangular test procedure, 20 subjects were repeatedly presented with all 21 binary combinations of the seven stimuli and asked to identify the bottle containing the odd stimulus. I found (a) that as a group, the subjects performed significantly above chance level in all tasks but two and thus were clearly able to discriminate between most of the odor pairs presented; (b) marked interindividual differences in discrimination performance, ranging from subjects who were able to significantly distinguish between all 21 odor pairs to subjects who failed to do so with 10 of the tasks; (c) that odor pairs involving two hexenols were significantly more difficult to discriminate than odor pairs that involved hexanol and one of the hexenols; (d) that odor pairs involving hexenols sharing the same geometry but differing in the position of the double bond by only one carbon atom were significantly more difficult to distinguish than odor pairs that involved hexenols differing by two carbon atoms; (e) that odor pairs involving 4-hexenols were significantly easier to discriminate than 3-hexenols, which, in turn, were significantly easier to distinguish than 2-hexenols; and (f) that odor pairs involving two cis-hexenols were significantly more difficult to discriminate than odor pairs that involved two trans-hexenols. These findings demonstrate that the presence as well as the position and configuration of a double bond affected discriminability in a systematic manner and suggest that these molecular structural features may be important determinants of the interaction between stimulus molecule and olfactory receptor and thus may affect odor quality of aliphatic alcohols.     

Rectal temperature, distal sweat rate, and forearm blood flow following mild exercise at two phases of the circadian cycle

Changes in rectal temperature during mild exercise in the middle of the rising (11:00 h) and falling (23:00 h) phases of the circadian rhythm of resting core temperature have been compared. Seven healthy males were studied at rest, while exercising on a cycle ergometer (60 min at 80 W), and during the first 30 min of recovery. Rectal temperature, forearm blood flow, and forearm sweat rate were measured at 1 min intervals throughout. During exercise, there were significant time-of-day differences in the profiles of all three variables, and in the thresholds for increases in forearm blood flow and sweating. Forearm blood flow and sweat rate were recruited more rapidly and to a greater extent with evening exercise, and rectal temperature rose less. Analysis of covariance, with rectal temperature as the covariate, indicated the associations between it and forearm blood flow or sweating were significantly different (p<0.05) between the two times of day. There were also significant (p<0.05) time-of-day effects for forearm blood flow and sweating that were independent of rectal temperature. During recovery, rectal temperature fell more quickly in the late evening than late morning. Forearm blood flow and sweating also showed time-of-day differences, but these did not co-vary with rectal temperature. Control of rectal temperature during exercise and recovery appears to be more effective in the late evening than late morning, and differences in forearm blood flow and sweating, as well as factors independent of these two variables, contribute to this difference. The results support our "heat-gain/heat-loss modes" hypothesis.     

Effect of time of day on line-bisecting performance in female soccer players

The purpose of this study was to examine the effect of time of day on visuospatial attention in trained subjects. We used the line-bisecting task. The protocol involved 30 healthy volunteer female soccer players (age: 21.1 ± 2.1 years, level of experience: 8.1 ± 3.2 years, height: 1.7 ± 0.03 cm, weight: 59.2 ± 3.49 kg). They performed two-test sessions at different times of day, 8:00 am and 8:00 pm. The results showed a significant time-of-day effect on line-bisecting performance, with a better precision in the evening than in the morning. The rightward error observed in the morning was reversed in the evening. An interaction between time of day x length was also observed, indicating that the difference in performance between the morning and the evening increased with the length of the line. We conclude that activities that require better visuospatial performance should be programed to take place in the evening.     

The effect of time of day on short-term maximal performance in obese and non-obese young men

The purpose of this study was to examine time-of-day effects on short-term performance in obese young men. In a balanced study design, 30 boys were divided into two groups: obese (OG; n = 15) and non-obese (CG; n = 15) groups. Both groups performed anaerobic tests of strength and power (squat-Jump, SJ; counter-movement jump, CMJ; 10-m sprint; and 30-m sprint) at 08:00 and 17:00 h on separate days. The results showed morning–evening differences in all variables in CG, with significant increases at 17:00 h (around the time of peak temperature) in comparison with 08:00 h (p < 0.01) for OG. However, such morning-evening differences were not found in the OG, except for the 30-m sprint. Obesity affects the diurnal variation on performances of 30-m sprint with a significant decrease in the evening for OG of 1.23% (p < 0.01). A correlation between anaerobic performance and oral temperature was observed only in the 30-m sprint test. In conclusion, the findings of the present study indicated that obesity is a problem that can affect circadian rhythms and performance in obese young men; therefore, preventing childhood obesity should become a top priority in efforts to improve performance and health.     

Olfactory discrimination ability of human subjects for enantiomers with an isopropenyl group at the chiral center

The ability of 20 human subjects to distinguish between nine enantiomeric odor pairs sharing an isopropenyl group at the chiral center was tested in a forced-choice triangular test procedure. I found (i). that as a group, the subjects were only able to significantly discriminate the optical isomers of limonene, carvone, dihydrocarvone, dihydrocarveol and dihydrocarvyl acetate, whereas they failed to distinguish between the (+)- and (-)-forms of perillaalcohol, perillaaldehyde, isopulegol and limonene oxide; (ii). marked interindividual differences in discrimination performance, ranging from subjects who were able to significantly discriminate between eight of the nine odor pairs to subjects who failed to do so with six of the nine tasks; and (iii). that with none of the nine odor pairs the antipodes were reported to differ significantly in subjective intensity when presented at equal concentrations. Additional tests of the chemesthetic potency and threshold measurements of the optical isomers of dihydrocarvone, dihydrocarveol, and dihydrocarvyl acetate suggest that the discriminability of these three enantiomeric odor pairs is indeed due to differences in odor quality. Analysis of structure-activity relationships suggest that the combined presence of (i). an isopropenyl group at the chiral center; (ii). a methyl group at the para-position; and/or (iii). an oxygen-containing group at the meta-position allows for the discrimination of enantiomeric odor pairs.     

Eveningness,Sleep Patterns,Daytime Functioning,and Quality of Life in Israeli Adolescents

The purpose of the study was to assess the relationships between eveningness, sleep patterns, measures of daytime functioning, i.e., sleepiness, sleep problem behaviors, and depressed mood, and quality of life (QOL) in young Israeli adolescents. A cross-sectional survey was performed in urban and rural middle schools in Northern Israel. Participants were 470 eighth and ninth grade middle school students (14?±?0.8 yrs of age) in the normative school system. Students completed the modified School Sleep Habits Survey (SSHS) and Pediatric Quality of Life Inventory Short Form, assessing six subscales of physical, emotional, social, school performance, and psychosocial functioning, plus an addition generated total score. During weekdays and weekends, evening types went to bed later, their sleep latency was longer, their wake-up time was later, and their sleep duration was shorter than intermediate and morning types. Evening types exhibited more sleep problem behaviors, sleepiness, depressed mood, and lower QOL compared to intermediate and morning types. Based on the regression model, sleepiness, sleep-problem behaviors, and depressed mood were the variables most strongly associated with QOL, followed by morning-evening preference, weekday sleep duration, and weekend sleep latency. This study is the first to assess QOL in normative, healthy adolescents and to demonstrate strong associations between morning-evening preference and QOL. These findings enhance the need to identify young individuals with an evening preference, and to be aware of the characteristics and manifestations of the evening chronotype on daytime and nighttime behaviors in adolescence. (Author correspondence: Orna@yvc.ac.il)     

The Isolated and Combined Effects of Menstrual Cycle Phase and Time-of-Day on Muscle Strength of Eumenorrheic Females

Diurnal variation in muscle performance has been well documented in the past few years, but almost exclusively in the male population. The possible effects of the menstrual cycle on human circadian rhythms have remained equivocal, particularly in the context of muscle strength. The purpose of the study was to analyze the isolated and combined effects of circamensal variation and diurnal changes on muscle strength. Eight eumenorrheic females (age 30 ± 5 yrs, height 1.63 ± 0.06 m and body mass 66.26 ± 4.6 kg: mean ± SD) participated in this investigation. Isokinetic peak torque of knee extensors and flexors of the dominant leg were measured at 1.05, 3.14 rad.s^?1 (through 90° ROM) at two times-of-day (06:00, 18:00 h) and five time points of the menstrual cycle (menses, mid-follicular, ovulation, mid-luteal, late luteal). In addition, maximum voluntary isometric contraction of knee extensors and flexors and electrically stimulated isometric contraction of the knee extensors were measured at 60° of knee flexion. Rectal temperature was measured during 30 min before the tests. There was a significant time-of-day effect on peak torque values for isometric contraction of knee extensors under electrical stimulation (P < 0.05). At 18:00 h, muscle force was 2.6% greater than at 06:00 h. The time-of-day effect was not significant when the tests were performed voluntarily without stimulation: effect size calculations indicated small differences between morning and evening for maximal voluntary isometric contraction and peak torque (at 1.05 rad.s^?1) for the knee extensors. A circamensal variation was observed for peak torque of knee flexors at 1.05 rad.s^?1, extensors at 3.14 rad.s^?1, and also isometric contraction of knee flexors, values being greatest at the ovulation phase. Interaction effects between time-of-day and menstrual cycle phase were not observed in any of the indices of muscle strength studied. The phase of the menstrual cycle seemed to have a greater effect than did the time-of-day on female muscle strength in this group of subjects. The present results suggest that peripheral rather than central mechanisms (e.g., motivation) are implicated in the diurnal variation of maximal isometric strength of women.     

Comparison of the Efficacy of Morning versus Evening Administration of Olmesartan in Uncomplicated Essential Hypertension

A total of 18 diurnally active subjects with uncomplicated, mild to moderate, essential hypertension were studied to compare the efficacy of the morning versus evening administration of an oral olmesartan medication. After a two‐week, wash‐out/placebo run‐in period, subjects with clinic diastolic blood pressure (DBP) ≥90 mm Hg and <110 mm Hg began 12 weeks of 20 mg olmesartan medoxomil tablet therapy at 08:00 h daily. Four of the 18 subjects required dose escalation to 40 mg at eight weeks because of clinic DBP≥90 mm Hg. After the 12‐week period of once‐a‐day 08:00 h treatment, subjects were immediately switched to an evening (20:00 h) drug‐ingestion schedule for another 12‐week period without change in dose. Subjects underwent 24 h ambulatory blood pressure monitoring (ABPM) before the initiation of morning treatment and at the end of both the 12‐week morning and evening treatment arms. Dosing time did not exert statistically significant differences on the efficacy of olmesartan: the reduction from baseline in the 24 h mean systolic (SBP) and DBP was, respectively, 18.8 and 14.6 mm Hg with morning dosing and 16.1 and 13.2 mm Hg with evening dosing (p>0.152 between groups). The amplitude of the BP 24 h pattern did not vary with dosing time, indicating full 24 h BP reduction no matter the clock hour of treatment. Although, the BP‐lowering effect was somewhat better with morning dosing, the results of this study suggest that the studied olmesartan medoxomil preparation efficiently reduces BP when ingested in the morning (08:00 h) or evening (20:00 h) in equivalent manner, based on statistical testing, throughout the 24 h.