共查询到20条相似文献,搜索用时 15 毫秒
1.
Xu J Ji C Wang L Cao Y Dai J Ye X Zeng L Dai J Wu Q Xie Y Mao Y 《Molecular biology reports》2003,30(1):47-52
We report here cloning and characterization of a novel human gene, termed C5orf12, which is a putative membrane protein belonging to the TMS_TDE family. The cDNA encodes 42 animo acid with a putative molecular weight of about 47 KDa. Secondary structure prediction showed that C5orf12 contained 10 putative transmembrane helices, which has high identity with other family members. We performed RT-PCR to examine its expression pattern. The result showed that C5orf12 was highly expressed in placenta, skeletal muscle, spleen, thymus, testis and peripheral leukocyte while expressed weakly in heart and liver. C5orf12 has high identity with the rat TPO1, so we speculate that C5orf12 may also have a role in the brain development. 相似文献
2.
Altered localization of amyloid precursor protein under endoplasmic reticulum stress 总被引:2,自引:0,他引:2
Kudo T Okumura M Imaizumi K Araki W Morihara T Tanimukai H Kamagata E Tabuchi N Kimura R Kanayama D Fukumori A Tagami S Okochi M Kubo M Tanii H Tohyama M Tabira T Takeda M 《Biochemical and biophysical research communications》2006,344(2):525-530
Recent reports have shown that the endoplasmic reticulum (ER) stress is relevant to the pathogenesis of Alzheimer disease. Following the amyloid cascade hypothesis, we therefore attempted to investigate the effects of ER stress on amyloid-beta peptide (Abeta) generation. In this study, we found that ER stress altered the localization of amyloid precursor protein (APP) from late compartments to early compartments of the secretory pathway, and decreased the level of Abeta 40 and Abeta 42 release by beta- and gamma-cutting. Transient transfection with BiP/GRP78 also caused a shift of APP and a reduction in Abeta secretion. It was revealed that the ER stress response facilitated binding of BiP/GRP78 to APP, thereby causing it to be retained in the early compartments apart from a location suitable for the cleavages of Abeta. These findings suggest that induction of BiP/GRP78 during ER stress may be one of the regulatory mechanisms of Abeta generation. 相似文献
3.
Yvonne Lange Jin Ye Theodore L Steck 《Biochemical and biophysical research communications》2002,290(1):488-493
Plasma membrane cholesterol both regulates and is regulated by effector proteins in the endoplasmic reticulum (ER) through a feedback system that is poorly understood. We now show that ER cholesterol varies over a fivefold range in response to experimental agents that act upon protein kinase C (PKC). Agents that activate Ca(2+)-dependent PKC [phorbol-12-myristate-13-acetate (PMA) and bryostatin 1] increased the level of ER cholesterol; inhibitors such as staurosporine and calphostin C decreased it. Rottlerin, a selective inhibitor of the PKC-delta isoform, also increased ER cholesterol. The esterification of plasma membrane cholesterol was altered by protein kinase C-directed agents in a corresponding fashion. Furthermore, the regulatory effect of plasma membrane cholesterol on the esterification of ER cholesterol was blocked by PKC-directed agents. These findings suggest that multiple protein kinase C isoforms participate in the regulation of ER cholesterol and therefore in cholesterol homeostasis. 相似文献
4.
Reiko Urade 《Bioscience, biotechnology, and biochemistry》2019,83(5):781-793
For most of the proteins synthesized in the endoplasmic reticulum (ER), disulfide bond formation accompanies protein folding in a process called oxidative folding. Oxidative folding is catalyzed by a number of enzymes, including the family of protein disulfide isomerases (PDIs), as well as other proteins that supply oxidizing equivalents to PDI family proteins, like ER oxidoreductin 1 (Ero1). Oxidative protein folding in the ER is a basic vital function, and understanding its molecular mechanism is critical for the application of plants as protein production tools. Here, I review the recent research and progress related to the enzymes involved in oxidative folding in the plant ER. Firstly, nine groups of plant PDI family proteins are introduced. Next, the enzymatic properties of plant Ero1 are described. Finally, the cooperative folding by multiple PDI family proteins and Ero1 is described. 相似文献
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6.
Jill B. Graham Nathan P. Canniff 《Critical reviews in biochemistry and molecular biology》2019,54(2):103-118
The endoplasmic reticulum (ER) is a complex, multifunctional organelle comprised of a continuous membrane and lumen that is organized into a number of functional regions. It plays various roles including protein translocation, folding, quality control, secretion, calcium signaling, and lipid biogenesis. Cellular protein homeostasis is maintained by a complicated chaperone network, and the largest functional family within this network consists of proteins containing tetratricopeptide repeats (TPRs). TPRs are well-studied structural motifs that mediate intermolecular protein–protein interactions, supporting interactions with a wide range of ligands or substrates. Seven TPR-containing proteins have thus far been shown to localize to the ER and control protein organization and homeostasis within this multifunctional organelle. Here, we discuss the roles of these proteins in controlling ER processes and organization. The crucial roles that TPR-containing proteins play in the ER are highlighted by diseases or defects associated with their mutation or disruption. 相似文献
7.
Viral glycoproteins fold and oligomerize in the endoplasmic reticulum of the host cell. They employ the cellular machinery and receive assistance from cellular folding factors. During the folding process, they are retained in the compartment and their structural quality is checked by the quality control system of the endoplasmic reticulum. A special characteristic that distinguishes viral fusion proteins from most cellular proteins is the extensive conformational change they undergo during fusion of the viral and cellular membrane. Many viral proteins fold in conjunction with and dependent on a viral partner protein, sometimes even synthesized from the same mRNA. Relevant for folding is that viral glycoproteins from the same or related virus families may consist of overlapping sets of domain modules. The consequences of these features for viral protein folding are at the heart of this review. 相似文献
8.
The endoplasmic reticulum is a major compartment of storage protein and lipid biosynthesis. Maximal synthesis of these storage compounds occurs during seed development with breakdown occurring during germination. In this study, we have isolated four independent preparations of ER from both developing and germinating seeds of castor bean (Ricinus communis) and used 2-D DIGE, and a combination of PMF and MS/MS sequencing, to quantify and identify differences in protein complement at both stages. Ninety protein spots in the developing seeds are up-regulated and 19 individual proteins were identified, the majority of these are intermediates of seed storage synthesis and protein folding. The detection of these transitory storage proteins in the ER is discussed in terms of protein trafficking and processing. In germinating seed ER 15 spots are elevated, 5 of which were identified, amongst them was malate synthetase which is a component of the glyoxysome which is believed to originate from the ER. Notably no proteins involved in complex lipid biosynthesis were identified in the urea soluble ER fraction indicating that they are probably all integral membrane proteins. 相似文献
9.
Yoshinori Kashiwayama Midori Seki Akina Yasui Masashi Morita Masao Sakaguchi Tsuneo Imanaka 《Experimental cell research》2009,315(2):190-557
70-kDa peroxisomal membrane protein related protein (P70R/ABCD4) is a member of ATP-binding cassette (ABC) protein subfamily D. ABC subfamily D proteins are also known as peroxisomal ABC proteins. Therefore, P70R is thought to be a peroxisomal membrane protein. However, the subcellular localization of P70R is not extensively investigated. In this study, we transiently expressed P70R in fusion with HA (P70R-HA) in CHO cells and examined subcellular localization by immunofluorescence. Surprisingly, P70R-HA was localized to the endoplasmic reticulum (ER), not to peroxisomes. To examine the ER-targeting property of P70R, we expressed various NH2-terminal deletion constructs of P70R. Among the NH2-terminal deletion constructs, mutant proteins starting with hydrophobic transmembrane segment (TMS) were localized to ER, but the ones containing the NH2-terminal hydrophilic cytosolic domain were not. ABC subfamily D proteins destined for peroxisomes have NH2-terminal hydrophilic region adjacent to TMS1. However, only P70R lacks the region and is translated with NH2-terminal hydrophobic TMS1. Furthermore, attachment of the NH2-terminal hydrophilic domain to the NH2-terminus of P70R excluded P70R from the ER-targeting pathway. These data suggest that P70R resides in the ER but not the peroxisomal membranes, and the hydrophobic property of NH2-terminal region determines the subcellular localization of ABC subfamily D proteins. 相似文献
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11.
Endoplasmic-reticulum-associated protein degradation inside and outside of the endoplasmic reticulum
Summary Newly synthesized polypeptides that enter the endomembrane system encounter a folding environment in the lumen of the endoplasmic reticulum (ER) constituted by enzymes, lectinlike proteins, and molecular chaperones. The folding process is under scrutiny of this abundant catalytic machinery, and failure of the new arrivals to assume a stable and functional conformation is met with targeting to proteolytic destruction, a process which has been termed ER-associated degradation (ERAD). In recent years it became clear that, in most cases, proteolysis appears to take place in the cytosol after retro-translocation of the substrate proteins from the ER, and to depend on the ubiquitin-proteasome pathway. On the other hand, proteolytic activities within the ER that have been widely neglected so far may also contribute to the turnover of proteins delivered to ERAD. Thus, ERAD is being deciphered as a complex process that requires communication-dependent regulated proteolytic activities within both the ER lumen and the cytosol. Here we discuss some recent findings on ERAD and their implications on possible mechanisms involved.Abbreviations lAT
alpha-1-antitrypsin
- apoB
apolipoprotein B
- BiP
immunoglobulin-heavy-chain-binding protein
- CFTR
cystic fibrosis transmembrane conductance regulator
- CPY
carboxypeptidase Y
- ER
endoplasmic reticulum
- ERAD ER
associated degradation
- HMG-CoA
3-hydroxy-3-methylglutaryl coenzyme A
- MHC
major histocompatibility complex
- PDI
protein disulflde isomerase
- TCR
T cell antigen receptor 相似文献
12.
The monoclonal antibody OL-1 and transmission electron microscopy were used to locate immunologically the major allergen of olive pollen. Ole e I, during pollen grain development. Within the pollen grain, allergenic proteins are located in the cisternae of the rough endoplasmic reticulum. Our findings indicate that the synthesis of these proteins starts in the vegetative cytoplasm of olive pollen during the early maturation stage.Dedicated to Professor Andreas Sievers on the occasion of his retirement 相似文献
13.
Abstract Co-translational translocation of Saccharomyces cerevisiae vacuolar glycoprotein carboxypeptidase Y (CpY) was highly efficient when studied with an in vivo and in vitro homologous system, comparison of limited proteolytic cleavage of immunoprecipitated translational products of CpY and subcellular localisation of a mutant CpY. The efficient segregation of CpY mRNA in highly purified fractions of rough microsomes was characterised. CpY1 mutant showed retention of core glycosylated material (proCpY1 ) in the rough and smooth endoplasmic reticulum fractions. It is suggested that the presence of structures that are incompatible with intercompartmental transport of vacuolar protein leads to retention of the mutated CpY by the endoplasmic reticulum. 相似文献
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15.
The ultrastructure of the storage parenchyma cells of the cotyledons of developing bean (Phaseolus vulgaris L.) seeds was examined in ultrathin frozen sections of specimens fixed in a mixture of glutaraldehyde, formaldehyde and acrolein, infused with 1 M sucrose, and sectioned at-80° C. Ultrastructural preservation was excellent and the various subcellular organelles could readily be identified in sections which had been stained with uranyl acetate and embedded in Carbowax and methylcellulose. The cells contained large protein bodies, numerous long endoplasmic reticulum cisternae, mitochondria, dictyosomes, and electron-dense vesicles ranging in size from 0.2 to 1.0 m. Indirect immunolabelling using rabbit immunoglobulin G against purified phaseolin (7S reserve protein), and ferritin-conjugated goat immunoglobulin G against rabbit immunoglobulin G was used to localize phaseolin. With a concentration of 0.1 mg/ml of anti-phaseolin immunoglobin G, heavy labeling with ferritin particles was observed ober the protein bodies, the cisternae of the endoplasmic reticulum, and the vesicles. The same structures were lightly labeled when the concentration of the primary antigen was 0.02 mg/ml. Ferritin particles were also found over the Golgi bodies. The absence of ferritin particles from other organelles such as mitochondria and from areas of cytoplasm devoid of organelles indicated the specificity of the staining, especially at the lower concentration of anti-phaseolin immunoglobulin G.Abbreviations ER
endoplasmic reticulum
- IgG
immunoglobulin G 相似文献
16.
Zou X Wang D Qiu G Ji C Jin F Wu M Zheng H Li X Sun L Wang Y Tang R Zhao RC Mao Y 《Biochemical genetics》2005,43(3-4):165-173
By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a novel human cDNA (C4orf13). This cDNA is 2706 bp in length, encoding a 340-amino-acid polypeptide that contains a typical SBF (sodium bile acid cotransporter family) domain and ten possible transmembrane segments. The putative protein C4orf13 shows high similarity with its orthologs in Mus musculus and Xenopus laevis. Human C4orf13 is mapped to chromosome 4q31.2 and contains 12 exons. RT-PCR analysis shows that human C4orf13 is widely expressed in human tissues, and the expression levels in liver and lung are relatively high, expression levels in placenta, kidney, spleen, and thymus are moderate, low levels of expression are detected in heart, prostate, and testis.The nucleotide sequence reported in this paper has been deposited to GenBank under accession number AY346324. 相似文献
17.
The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress 总被引:23,自引:0,他引:23
Lee AS 《Methods (San Diego, Calif.)》2005,35(4):373-381
18.
Induction of apoptosis by adenovirus E4orf4 protein 总被引:2,自引:0,他引:2
Adenovirus E4orf4 protein is a multifunctional viral regulator that induces p53-independent apoptosis in transformed cells, but not in normal cells. E4orf4-induced apoptosis can occur without activation of known caspases, although E4orf4 induces caspase activity in some cell lines. The interaction of E4orf4 with a specific subpopulation of protein phosphatase 2A (PP2A) molecules that contain B subunits, but not with those that contain B subunits, is required for induction of apoptosis. This review suggests the potential use of E4orf4 in cancer therapy, and discusses whether E4orf4-induced apoptosis plays a role in the viral life cycle. Future research directions are also highlighted. 相似文献
19.
Retention of maize auxin-binding protein in the endoplasmic reticulum: quantifying escape and the role of auxin 总被引:5,自引:0,他引:5
J. Henderson J. M. Bauly D. A. Ashford S. C. Oliver C. R. Hawes C. M. Lazarus M. A. Venis R. M. Napier 《Planta》1997,202(3):313-323
The localisation of maize (Zea mays L.) auxin-binding protein (ABP1) has been studied using a variety of techniques. At the whole-tissue level, tissue printing
indicated that ABP1 is expressed to similar levels in all cells of the maize coleoptile and in the enclosed leaf roll. Within
cells, the signals from immunofluorescence and immunogold labelling of ultrathin sections both indicated that ABP1 is confined
to the endoplasmic reticulum (ER), none being detected in either Golgi apparatus or cell wall. This distribution is consistent
with targeting motifs in its sequence. These observations are discussed with reference to the various reports which place
a population of ABP1 on the outer face of the plasma membrane, including those suggesting that it is necessary on the cell
surface for rapid, auxin-mediated protoplast hyperpolarisation. We have tested one proposed model to account for release of
ABP1 from the ER, namely that auxin binding induces a conformational change in ABP1 leading to concealment of the KDEL retention
motif. Using double-label immunofluorescence the characteristic auxin-induced rise in Golgi-apparatus signal was found, yet
no change in the distribution of the ABP1 signal was detected. Maize suspension cultures were used to assay for auxin-promoted
secretion of ABP1 into the medium, but secretion was below the limit of detection. This can be ascribed at least partly to
the very active acidification of the medium by these cells and the instability of ABP1 in solution below pH 5.0. In the insect-baculovirus
expression system, in which cell cultures maintain pH 6.2, a small amount of ABP1 secretion, less than 1% of the total, was
detected under all conditions. Insect cells were shown to take up auxin and no inactivation of added auxin was detected, but
auxin did not affect the level of ABP1 in the medium. Consequently, no evidence was found to support the model for auxin promotion
of ABP1 secretion. Finally, quantitative glycan analysis was used to determine what proportion of ABP1 might reach the plasma
membrane in maize coleoptile tissue. The results suggest that less than 15% of ABP1 ever escapes from the ER as far as the
cis-Golgi and less than 2% passes further through the secretory pathway. Such leakage rates probably do not require a specialised
mechanism allowing ABP1 past the KDEL retrieval pathway, but we are not able to rule out the possibility that some ABP1 is
carried through associated with other proteins. The data are consistent with the presence of ABP1 both on the plasma membrane
and in the ER. The relative sizes of the two pools explain the results obtained with immunofluorescence and immunogold labelling
and illustrate the high efficiency of ER retention in plants.
Received: 31 October 1996 / Accepted: 16 December 1996 相似文献