共查询到20条相似文献,搜索用时 15 毫秒
1.
M. Locke 《Journal of morphology》1969,127(1):7-39
A structure for a generalized insect epidermal cell during the formation of the epicuticle is proposed, based on studies of several different epidermal cell types. The protein epicuticle is defined as the dense homogeneous layer below the cuticulin. The formation of the protein epicuticle involves secretory vesicles arising in Golgi complexes, and marks an interlude in the involvement in cuticle formation of plasma membrane plaques. The plaques are concerned in cuticulin formation before and in fibrous cuticle formation after the deposition of the protein epicuticle. The epidermis is characterized by the possession of a cytoskeleton of microtubules and a matrix of microfibers. In the elongated cells forming bristles and spines, the microfibers are often oriented in bundles with an axial banding which repeats every 120 Å. The microtubules are also arranged in columns with a trigonal packing and center to center spacing of about 800 Å. These cytoskeletal structures separate the other organelles into channels which may restrict the pathways open for the movement of secretory and pinocytotic vesicles. The protein epicuticle arises from the secretory vesicles which discharge at the apical surface. The contents disperse and reaggregate below the cuticulin. The Golgi complexes in the basal and central regions have many secretory vesicles and a small saccular component, differing from those nearer the apex which are smaller and have fenestrated saccules. The small coated vesicles (800 Å in diameter) associated with both sorts of complex, probably move to the apical and basal faces of the cell where they may give rise to the large coated vesicles (2000 Å in diameter) inserted in the plasma membrane. Pinocytosis occurs from both apical and basal faces but most lytic activity is in the apical region. Plant peroxidase injected into the haemocoel is taken up basally and transported to the apical MVBs. The large coated vesicles on the apical face may be concerned in the control of the extracellular subcuticular environment. They appear to fill up and detach, fusing to become the apical MVBs. 相似文献
2.
The fine structure and deposition of the larval cuticle of the sheep blowfly (Lucilia Cuprina) 总被引:1,自引:0,他引:1
Filshie BK 《Tissue & cell》1970,2(3):479-498
The cuticle of Lucilia is composed of an untanned endocuticle and a complex epicuticle of four layers, superficial layer, outer epicuticle, cuticulin and dense layer. The outer epicuticle and attached epicuticular filaments are resistant to acid hydrolysis. During deposition of the cuticle of each larval instar, the cuticulin and dense layers are formed first, followed by the outer epicuticle, which appears to be laid down by secretions from the epidermis passing through the cuticulin via epicuticular filaments. The outer epicuticle is found in the position normally occupied by the wax layer of other insect species. 相似文献
3.
The hormonal coordination of cuticulin deposition and morphogenesis in Drosophila imaginal discs in vivo and in vitro 总被引:1,自引:0,他引:1
Cuticulin is the first layer of the insect cuticle to be deposited and is laid down as a continuous inelastic sheet over the apical surface of cuticle-secreting cells. During metamorphosis in Drosophila melanogaster, imaginal discs deposit the cuticulin layer of the pupal cuticle between 3 and 7 hr after puparium formation. This is a period of rapid morphogenesis involving cell shape changes and cell rearrangements. We have examined cuticulin deposition in vivo and in vitro with a view to understanding the coordination of cuticulin deposition with morphogenesis. We find that the optimum hormonal regimen (of the steroid hormone, 20-hydroxyecdysone) for the completion of both morphogenesis and cuticulin deposition in vitro parallels the changes in hormone titer observed in vivo. We also find that cuticulin is deposited last over cell boundaries, thereby allowing cell rearrangements to occur as cuticulin is laid down. We have identified in vitro conditions under which cuticulin deposition is completed precociously, inhibiting further morphogenesis. Cytochalasin B and colchicine do not inhibit cuticulin deposition and we therefore conclude that an intact cytoskeleton is not necessary for secretion of this extracellular structure. Finally, we present a preliminary protocol for the partial purification of cuticulin synthesized in vitro by mass isolated discs. 相似文献
4.
In mayfly nymphs of the genus Coloburiscoides, cell complexes with an osmoregulatory function (so-called chloride cells) are found in the integuments of the oral gills, the abdominal gills and gill filaments, the coxae and the thoracic sternites. The cuticle overlying each cell complex is a rigid circular plate which is known to be porous to colloidal lanthanum suspensions. The present study shows that the plate is composed only of the cuticulin and dense layers of the epicuticle. Both layers have substructures built of subunits on almost perfect hexagonal lattices. The lattice spacings are 53 and 9.5 nm for the dense layer and the cuticulin layer respectively. During moulting the apical plasma membrane of the chloride cell remains adpressed to the old porous plate. The new porous plate is formed from a new chloride cell which intrudes from the base of the integument. Throughout the moult small pores persist in the new and otherwise continuous cuticle to allow continuity of the cytoplasm of the apical and basal portions of the old chloride cell. It is thought that this phenomenon allows osmoregulatory function of the chloride cell complex to be maintained during the moult. 相似文献
5.
Rinterknecht E 《Tissue & cell》1985,17(5):723-743
During late embryogenesis in a cockroach, the epidermal cells secrete two cuticles: the embryonic cuticle and the pharate first larval cuticle. Late embryogenesis begins with the deposition of the cuticulin layer of the embryonic cuticle. The embryonic cuticle is an atypical one. It remains relatively thin and a well lamellated endocuticle is usually lacking. After general apolysis of the embryonic cuticle the epidermis secretes the epicuticle of the first larval cuticle and, subsequently, a typical lamellate procuticle. During the penultimate phase of late embryogenesis (i.e. before general apolysis) the epidermis becomes larvally committed. Some epidermal cells start to differentiate into specialized structures of the dermal glands, whereas the differentiated oenocytes appear to have acquired some stability. Nevertheless, shortly before general apolysis some oenocytes display signs of an increased alteration of the SER. When general apolysis occurs, the oenocytes contain a well-developed SER. The whole of the oenocyte population is programmed to regress after epicuticle deposition of the first larval cuticle. The correlation of oenocyte regression with available data on cuticulogenesis, ecdysteroid titres and cuticular lipid synthesis is discussed. 相似文献
6.
为了探明20-羟基蜕皮甾酮对昆虫蜕皮过程中体壁的表皮层、 皮细胞及其细胞器的具体影响过程, 本研究利用透射电镜技术研究了20-羟基蜕皮甾酮对舞毒蛾Lymantria dispar (Linnaeus)5龄幼虫体壁超微结构的变化。结果表明, 用高浓度20-羟基蜕皮甾酮溶液浸过的白桦叶片饲喂幼虫, 处理6 h, 摄入约400 μg 20-羟基蜕皮甾酮后, 幼虫停止取食; 处理12 h时表皮细胞顶膜上的微绒毛减少, 在皮细胞与旧表皮之间形成蜕皮间隙, 旧头壳从幼虫头部脱离; 处理24 h时蜕皮间隙继续增大, 旧表皮与皮细胞进一步分离, 新表皮质层开始形成; 处理36 h时皮细胞顶膜形成较短的微绒毛, 胞质区域出现数量较多的电子疏松泡, 新表皮由上表皮、 外表皮及8层左右内表皮片层组成; 处理48 h时顶膜与内表皮界限模糊, 内表皮继续合成至16层左右; 72 h时细胞内出现大面积电子疏松泡, 内表皮合成至20层左右。 处理96 h时, 与对照组相比, 皮细胞细胞器较少, 核仁周围出现小部分空白区域, 胞质区域内含物减少; 虫体发黑缩小, 即将死亡; 内表皮层数仍旧保持20层左右。对照组幼虫6-96 h虫体活跃, 正常取食, 外部观察及透射电镜结果均未显现蜕皮现象; 表皮层由上表皮、 外表皮及内表皮组成; 皮细胞顶膜微绒毛密度高; 表皮细胞分泌活动旺盛, 胞质区域细胞界限明显, 内含物丰富; 细胞器典型而且活跃; 内表皮片层随时间不断增加至50层左右。结果提示, 外源20-羟基蜕皮甾酮能够导致舞毒蛾5龄幼虫的致死性蜕皮。 相似文献
7.
Shatrov AB 《Tsitologiia》2000,42(3):219-227
The ultrastructural organization of hypodermis and the process of cuticle deposition is described for the pharate larvae of a trombiculid mite, Leptotrombidium orientale, being under the egg-shell and prelarval covering. The thin single-layered hypodermis consists of flattened epithelial cells containing oval or stretched nuclei and smooth basal plasma membrane. The apical membrane forms short scarce microvilli participating in the cuticle deposition. First of all, upper layers of the epicuticle, such as cuticulin lamella, wax and cement layers, are formed above the microvilli with plasma membrane plaques. Cuticulin layer is seen smooth at the early steps of this process. Very soon, however, epicuticle starts to be curved and forms particular high and tightly packed ridges, whereas the surface of hypodermal cells remains flat. Then a thick layer of the protein epicuticle is deposited due to secretory activity of hypodermal cells. Nearly simultaneously the thick lamellar procuticle starts to form through the deposition of their microfibrils at the tips of microvilli of the apical plasma membrane. Procuticle, as such, remains flat, is situated beneath the epicuticular ridges and contains curved pore canals. Cup-like pores in the epicuticle provide augmentation of the protein epicuticle mass due to secretion of particular substances by cells and to their transportation through the pore canals towards these epicuticular pores. The very beginning of the larval cuticle formation apparently indicates the starting point of the larval stage in ontogenesis, even though it remains for some time enveloped by the prelarval covering or sometimes by the egg-shell. When all the processes of formation are over, hungry larvae with a fully formed cuticle are actively hatched from two splitted halves of prelarval covering. 相似文献
8.
Light and transmission electron microscopy were used to monitor changes due to the degradation of the old exoskeleton and related events in the sclerites, articular membranes, and gills of two decapod crustaceans (Carcinus maenas and Macropipus puber) during pre-ecdysis. In both sclerites and articular membranes, degradation follows a similar general pattern in both crab species, while the gill cuticle appears unaltered. In early pre-ecdysis (D(0)), the degradation of the old cuticle starts with the secretion of ecdysial droplets by the epidermis. Apolysis, occurring at stage D(1)', is re-defined as an event, not necessarily morphologically observable, consisting in the loss of adherence between the epidermis and the old cuticle during early pre-ecdysis of arthropods. At the stage D(1)', the moulding of the epidermal cell surface occurs in preparation to the deposition of the new cuticle and causes the opening of the ecdysial cleft. In the principal layer of sclerites, degradation of the chitin-protein microfibres should precede mineral dissolution. In contrast to the other degraded cuticle layers, the membranous layer of sclerites and the innermost endocuticular lamellae of articular membranes are transformed into a digestion-resistant fibrous network resembling the ecdysial membrane of insects. 相似文献
9.
Insect cuticle forms as a result of overlapping sequences of two kinds of process, those involving vesicles of the Golgi complex, and those related to transport through and/or assembly at the apical plasma membrane. The ecdysial droplets are the last layer of old cuticle to be deposited before ecdysis and form from the contents of secretory vesicles from Golgi complexes. Ecdysial droplets and secretory vesicles both stain with PTA and react with silver hexamine after oxidation with periodic acid. The vesicles discharge in localized apical areas devoid of microvilli where they accumulate as droplets measuring about 3 [ x 1 [. The. droplets span the last few lamellae of the endocuticle which becomes the ecdysial membrane. They dissolve to leave the ecdysial membrane full of holes at the time that the rest of the old cuticle is digested. 相似文献
10.
In Manduca sexta the germ band is formed 12 h post-oviposition (p.o.) (=10% development completed) and is located above the yolk at the egg surface. The cells show a polar organization. They are engaged in the uptake and degradation of yolk globules, pinched off from the yolk cells. This process can be observed in the integumental cells during the first growth phase of the embryo that lasts until "katatrepsis," an embryonic movement that takes place at 40% development completed. At 37% development completed, the ectoderm deposits a thin membrane at its apical surface, the first embryonic membrane, which detaches immediately before katatrepsis. The second period of embryonic growth--from katatrepsis to 84 h p.o. (70% development completed)--starts with the deposition of a second embryonic membrane that is somewhat thicker than the first one and shows a trilaminar, cuticulin-like structure. Whereas the apical cell surface is largely smooth during the deposition of the first embryonic membrane, it forms microvilli during deposition of the second one. At the same time, uptake of formed yolk material ceases and the epidermal cells now contain clusters of mitochondria below the apical surface. Rough endoplasmic reticulum (RER) increases in the perinuclear region. The second embryonic membrane detaches about 63 h p.o. At 69 h p.o., a new generation of microvilli forms and islands of a typical cuticulin layer indicate the onset of the deposition of the larval cuticle. The third growth phase is characterized by a steady increase in the embryo length, the deposition of the larval procuticle, and by cuticular tanning at about 100 h p.o. Beginning at that stage, electron-lucent vesicles aggregate below the epidermal surface and are apparently released below the larval cuticle. Manduca sexta is the first holometabolous insect in which the deposition of embryonic membranes and cuticles has been examined by electron microscopy. In correspondence with hemimetabolous insects, the embryo of M. sexta secretes three covers at approximately the same developmental stage. A marked difference: the second embryonic cover, which in Hemimetabola clearly exhibits a cuticular organization, has instead a membranous, cuticulin-like structure. We see the difference as the result of an evolutionary reductional process promoted by the redundancy of embryonic covers in the egg shell. Embryonic "molts" also occur in noninsect arthropods; their phylogenetical aspects are discussed. 相似文献
11.
Ursula Mothes-Wagner 《Zoomorphology》1984,104(2):105-110
Summary Processes occurring during moulting in Tetranychus urticae (Acari, Tetranychidae) are described by means of electron microscopy.Moulting is characterized by a pre-ecdysial phase which is initiated by the detachment of cuticle and epidermis. Epicuticular material is deposited as plaques but fuses to form a continuous layer. The epidermis folds up and ridges become determined. Procuticular material is synthesized inside the epidermis and packed into granules which accumulate below the epicuticular portions already deposited. Prior to ecdysis, portions of the old cuticle are dissolved. Ecdysis is achieved by moulting glands which effect bursting of the old cuticle. During the post-ecdysial phase, the endocuticle is synthesized during which a lamellation becomes obvious.Processes occuring during moulting are compared to published information on the tick cuticle. 相似文献
12.
The Golgi complexes of animal cells are said to become vesicular during cell division in order to allow the equal partitioning of organelles between daughter cells (Warren, 1985). However, in the epidermis of fifth stage larval Calpodes ethlius (Lepidoptera, Hesperi idae), cutical deposition is concurrent with cell division in preparation for pupation. We therefore looked at the Golgi complexes of these epidermal cells to see if they maintained their interphase form to allow them to continue to function during cell division. Dividing cells were recognized by changes in the nucleus and nuclear envelope, the form of the cell cortex and cell surface, and by the disposition of microtubules. Epidermal Golgi complexes consist of 3-5 cisternae capped by endoplasmic reticulum with transfer vesicles and rings of GC beads next to the cis face, and secretory vesicles on the trans face. Golgi complexes of dividing cells are structurally indistinguishable from those in interphase, their beads are in the rings characteristic of active GCs, and cuticle continues in uninterrupted lamellae above the apical microvilli. The observations suggest that Golgi complexes in dividing insect cells differ from those of most vertebrates by remaining functional through mitosis. 相似文献
13.
The distribution of phenoloxidase and polyphenols have been studied during cuticle formation at the 4th to 5th molt in Colpodes ethius. Cuticular phenoloxidases arise in the epidermis in cisternae of the rough endoplasmic reticulum, pass through the Golgi complex and are transported to the apical face in secretory vesicles. From the cuticular environment some enzyme is pinocytosed and broken down in the apical multivesicular bodies. Phenoloxidase and polyphenols are present during the formation of the cuticulin layer which also reacts as if it were at least partly composed of a phenoloxidase. The rest of the epicuticle incorporates phenoloxidase as it is deposited, particularly that over the dorsal tubercles which later melanize. Polyphenols do not appear until shortly before ecdysis. They are associated with the epicuticular filaments in both epicuticle and presumptive epocuticle. It is proposed that the epicuticular filaments may arise as liquid crystals with a protein component which becomes stabilized like the rest of the cuticle. These structures could provide a channel for the movement of both lipids and quinones to the surface. Phenoloxidases may pass through fibrous cuticle to be deposited as part of the epicuticle but are incorporated in fibrous cuticle scheduled for sclerotization. The time of stabilization is determined by the availability of polyphenols. 相似文献
14.
To establish a base for studying the hormonal control of insect epidermal cell activity, the ultrastructure of abdominal epidermis was analyzed during the normal pupal-adult development of Hyalophora cecropia. Adjacent epidermal cells could be distinguished on the basis of organelle content and staining intensity, suggesting that this monolayer is not composed of a homogenous cell population. At the onset of adult development the form of the epidermal cell is transformed from that typical of a quiescent cell with free ribosomes and few mitochondria to one which is metabolically active and possesses numerous apical membrane microvilli, rough endoplasmic reticulum and numerous mitochondria. On about day 5 of pharate adult development the apical plasma membrane is no longer folded but becomes folded again several days later when cuticulin and endocuticle are deposited. On about day 7, giant autophagic vacuoles are discerned that may be important in cellular reprogramming. After adult ecdysis, the epidermal cells continue to deposit endocuticle. 相似文献
15.
Angelika B?ttger Andrew C. Doxey Michael W. Hess Kristian Pfaller Willi Salvenmoser Rainer Deutzmann Andreas Geissner Barbara Pauly Johannes Altst?tter Sandra Münder Astrid Heim Hans-Joachim Gabius Brendan J. McConkey Charles N. David 《PloS one》2012,7(12)
The single-cell layered ectoderm of the fresh water polyp Hydra fulfills the function of an epidermis by protecting the animals from the surrounding medium. Its outer surface is covered by a fibrous structure termed the cuticle layer, with similarity to the extracellular surface coats of mammalian epithelia. In this paper we have identified molecular components of the cuticle. We show that its outermost layer contains glycoproteins and glycosaminoglycans and we have identified chondroitin and chondroitin-6-sulfate chains. In a search for proteins that could be involved in organising this structure we found PPOD proteins and several members of a protein family containing only SWT (sweet tooth) domains. Structural analyses indicate that PPODs consist of two tandem β-trefoil domains with similarity to carbohydrate-binding sites found in lectins. Experimental evidence confirmed that PPODs can bind sulfated glycans and are secreted into the cuticle layer from granules localized under the apical surface of the ectodermal epithelial cells. PPODs are taxon-specific proteins which appear to have entered the Hydra genome by horizontal gene transfer from bacteria. Their acquisition at the time Hydra evolved from a marine ancestor may have been critical for the transition to the freshwater environment. 相似文献
16.
Joan Lai-Fook 《Journal of morphology》1967,123(4):503-527
The relationship between muscles and the components of the integument in muscle insertions have been studied with the electron microscope in two insects, Calpodes ethlius (Hesperiidae, Lepidoptera) and Rhodnius prolixus (Reduviidae, Hemiptera). The area of contact between the muscles and the epidermis is increased by interdigitating processes whose membranes are joined by intermediate junctions. The junctions occur at the level of a Z line so that actin filaments attach directly to them. Within the epidermis, microtubules extend from the junctions of the myoepidermal connection to the cuticle, where they attach to hemidesmosomes which line deep indentations of the membrane. The microtubules probably enable the tendinous epidermal cells to withstand the tensions exerted upon them by the muscles. The epidermis is anchored to the cuticle by tonofibrillae, homogeneous rods secreted in the deep indentations of the plasma membrane. Since the tonofibrillae of successive instars are continous, they penetrate and attach to the cuticulin, the outermost layer of the epicuticle. 相似文献
17.
M E Greenstein 《Journal of morphology》1972,136(1):1-21
The ultrastructure of wing epidermis of the giant silkmoth, Hyalophora cecropia, was studied during pupal diapause and the first half of development to the adult. In diapause, the generalized epidermal cells are characterized by many free ribosomes, some vesicles and small lamellae of rough endoplasmic reticulum, some scattered short mitochondria and a few small Golgi complexes. During the early states of post-diapause development, before and after the time of apolysis (separation of the epidermis from the overlying cuticle), there is a marked increase in structures often associated with synthetic functions, such as polyribosomes, lamellate rough endoplasmic reticulum and Golgi complexes. On day five of post-apolysis development, just after the appearance of scale-forming and socket-forming cells, the generalized epidermal cells lay down the cuticulin layer of the adult cuticle. At this stage and later, the polyribosomes and lamellate rough endoplasmic reticulum decrease in abundance. Cell nuclei show three phases of temporary transition from predominantly lobed to predominantly round profile, which correspond to periods of reported DNA synthesis. Throughout this developmental process, therefore, there is good correlation of fine structure with changes in macromolecular synthesis recorded elsewhere. 相似文献
18.
Structure of the cuticle of the alpine weta,Hemideina maori (Saussure) (Orthoptera: Stenopelmatidae)
《International Journal of Insect Morphology and Embryology》1989,18(1):1-11
Sclerotized cuticle segments from the thorax, dorsal abdomen, and ventral abdomen of the alpine, weta Hemideina maori (Saussure) (Orthoptera: Stenopelmatidae) were examined by light microscopy and by scanning and transmission electron microscopy. An epicuticle, exocuticle (outer and inner), mesocuticle, endocuticle, and deposition layer are present in transverse sections. The epicuticle is further composed of a cuticulin layer and inner epicuticle, the latter being finely laminated and containing narrow wax canals that terminate below the cuticle surface. Openings to dermal gland ducts are visible on the surface as are large setae and smaller sensory pegs. Frozen fractured cuticle reveals the presence of horizontal ducts or channels that run laterally within the cuticle. The structure of weta cuticle is compared with that of the common house cricket and arthropods in general. 相似文献
19.
The structure and formation of the cuticulin layer in the epicuticle of an insect, Calpodes ethlius (Lepidoptera, Hesperiidae) 总被引:2,自引:0,他引:2
M Locke 《Journal of morphology》1966,118(4):461-494
The cuticulin layer is defined as the dense lamina (120–175 Å thick in Calpodes larvae, depending upon the stage) forming the outer part of the epicuticle in insects. It completely invests an insect except for the gut and the openings of some sense organs. It is the first layer to be secreted during the formation of new cuticle. The formation of the cuticulin membrane may be a useful model for studying the origin of membranes in general. It arises as a triple layer de novo and is not a modified plasma membrane. Growth is by accretion at the edges of patches of cuticulin which increase in area until they cover the new surface. The triple layer (i.e. three dense laminae) may develop striations about 30 Å apart transverse to the membrane, which perhaps form a sieve allowing small molecules to pass while protecting the cell from enzymes in the molting fluid. A similar porous structure persists in the tracheoles. After the resorption of molting fluid the triple layered structure again becomes obvious and the outermost layer separates from the other two to become what may be the surface lipid monolayer. The surface patterns in cuticle of various sorts probably arise by buckling of the cuticulin layer as it increases in surface area. 相似文献
20.
Andreas Bubel 《Journal of experimental marine biology and ecology》1975,20(3):287-324
The fine structure of the mantle and shell of the barnacle, Elminius modestus Darwin has been examined by electron microscopy. The epithelial cells along the outer face of the mantle differ in size, shape, and organelle complexity according to the different components of the shell they secrete. The shell consists of a non-calcareous basis and calcareous mural and opercular plates which are connected by a flexible opercular hinge. Both the basis and opercular hinge are composed of two main units: an outer cuticulin layer and a lamellate component of well ordered arched fibrils. During the deposition of the latter structures morphological changes in the cells occur which may be correlated with the moulting cycle. Preliminary results show that the calcareous plates are covered by an outer epicuticle, which is bordered by a cuticulin layer; the inner calcareous component, consists of an orderly arrangement of organic matrix envelopes within which crystals may be initiated.
The cells lining the inner surface of the mantle are uniform in appearance with a thin cuticle at their free surface which lines the body cavity. The latter structure of the cuticle and manner of its deposition are similar to those of the basis and opercular hinge. Separating the outer and inner mantle epithelial cells is connective tissue which comprises several differing cell types. The possibilities are discussed of the rôle these cells may play in shell deposition. The modes by which underlying cells secrete the different shell components and the cuticle lining the inner face of the mantle, are also discussed. 相似文献