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1.
Functional markers in wheat: current status and future prospects 总被引:10,自引:0,他引:10
Liu Y He Z Appels R Xia X 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,125(1):1-10
Functional markers (FM) are developed from sequence polymorphisms present in allelic variants of a functional gene at a locus. FMs accurately discriminate alleles of a targeted gene, and are ideal molecular markers for marker-assisted selection in wheat breeding. In this paper, we summarize FMs developed and used in common wheat. To date, more than 30 wheat loci associated with processing quality, agronomic traits, and disease resistance, have been cloned, and 97 FMs were developed to identify 93 alleles based on the sequences of those genes. A general approach is described for isolation of wheat genes and development of FMs based on in silico cloning and comparative genomics. The divergence of DNA sequences of different alleles that affect gene function is summarized. In addition, 14 molecular markers specific for alien genes introduced from common wheat relatives were also described. This paper provides updated information on all FMs and gene-specific STS markers developed so far in wheat and should facilitate their application in wheat breeding programs. 相似文献
2.
Merethe Bagge Thomas Lübberstedt 《Molecular breeding : new strategies in plant improvement》2008,22(3):319-328
Speed and cost–effective techniques for evaluation of plant materials to provide information before entering the next cycle
of selection are critical for success in plant breeding. Whether or not a ‘new’ technique realizes its potential depends on
technical and economic considerations. Biotechnology-based research tools such as doubled haploid technology and molecular
markers have already demonstrated their value for application in plant breeding. In the area of genomics, implementation of
functional markers (FMs) is currently of particular interest. The pipeline from plant materials to FM data points for any
application includes maceration of plant material, DNA isolation and sample preparation. For each step of this pipeline, a
number of techniques are available, and no single method is ideally suited for all applications. The challenge is to meet
the needs of many different scenarios which are present in a modern breeding programme such as the use of (1) few markers
for genotyping hundreds of samples (e.g., marker assisted backcrossing (MAB)), (2) few markers in thousands of samples (e.g.,
screening for GMO), (3) hundreds to thousands of markers for hundreds to thousands of samples (e.g., genetic characterization
of breeding materials (fingerprinting)). This paper compares different techniques for each of the steps from plant material
to FM data point, with the main emphasis on SNP detection platforms, assuming that multiple FMs will become available in the
near future. We focus on technical and economic aspects and discuss which techniques are most suitable for each of the scenarios
using wheat as a model. 相似文献
3.
Raboin LM Pauquet J Butterfield M D'Hont A Glaszmann JC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(5):701-714
Linkage disequilibrium (LD) in crops, established by domestication and early breeding, can be a valuable basis for mapping the genome. We undertook an assessment of LD in sugarcane (Saccharum spp), characterized by one of the most complex crop genomes, with its high ploidy level (>or=8) and chromosome number (>100) as well as its interspecific origin. Using AFLP markers, we surveyed 1,537 polymorphisms among 72 modern sugarcane cultivars. We exploited information from available genetic maps to determine a relevant statistical threshold that discriminates marker associations due to linkage from other associations. LD is very common among closely linked markers and steadily decreases within a 0-30 cM window. Many instances of linked markers cannot be recognized due to the confounding effect of polyploidy. However, LD within a sample of cultivars appears as efficient as linkage analysis within a controlled progeny in terms of assigning markers to cosegregation groups. Saturating the genome coverage remains a challenge, but applying LD-based mapping within breeding programs will considerably speed up the localization of genes controlling important traits by making use of phenotypic information produced in the course of selection. 相似文献
4.
Linkage disequilibrium in domestic sheep 总被引:15,自引:0,他引:15
The last decade has seen a dramatic increase in the number of livestock QTL mapping studies. The next challenge awaiting livestock geneticists is to determine the actual genes responsible for variation of economically important traits. With the advent of high density single nucleotide polymorphism (SNP) maps, it may be possible to fine map genes by exploiting linkage disequilibrium between genes of interest and adjacent markers. However, the extent of linkage disequilibrium (LD) is generally unknown for livestock populations. In this article microsatellite genotype data are used to assess the extent of LD in two populations of domestic sheep. High levels of LD were found to extend for tens of centimorgans and declined as a function of marker distance. However, LD was also frequently observed between unlinked markers. The prospects for LD mapping in livestock appear encouraging provided that type I error can be minimized. Properties of the multiallelic LD coefficient D' were also explored. D' was found to be significantly related to marker heterozygosity, although the relationship did not appear to unduly influence the overall conclusions. Of potentially greater concern was the observation that D' may be skewed when rare alleles are present. It is recommended that the statistical significance of LD is used in conjunction with coefficients such as D' to determine the true extent of LD. 相似文献
5.
Hordeum chilense is a potential source of useful genes for wheat breeding. The use of this wild species to increase genetic variation in wheat will be greatly facilitated by marker-assisted introgression. In recent years, the search for the most suitable DNA marker system for tagging H. chilense genomic regions in a wheat background has lead to the development of RAPD and SCAR markers for this species. RAPDs represent an easy way of quickly generating suitable introgression markers, but their use is limited in heterogeneous wheat genetic backgrounds. SCARs are more specific assays, suitable for automatation or multiplexing. Direct sequencing of RAPD products is a cost-effective approach that reduces labour and costs for SCAR development. The use of SSR and STS primers originally developed for wheat and barley are additional sources of genetic markers. Practical applications of the different marker approaches for obtaining derived introgression products are described. 相似文献
6.
Saravanan Thavamanikumar Simon G. Southerton Gerd Bossinger Bala R. Thumma 《Tree Genetics & Genomes》2013,9(3):627-639
Due to their long reproductive cycles and the time to expression of mature traits, marker-assisted selection is particularly attractive for tree breeding. In this review, we discuss different approaches used for developing markers and propose a method for application of markers in low linkage disequilibrium (LD) populations. Identification of useful markers for application in tree breeding is mainly based on two approaches, quantitative trait locus (QTL) mapping and association genetic studies. While several studies have identified significant markers, effect of the individual markers is low making it difficult to utilize them in breeding programs. Recently, genomic selection (GS) was proposed for overcoming some of these difficulties. In GS, high density markers are used for predicting phenotypes from genotypes. Currently small effective populations with high LD are being tested for GS in tree breeding. For wider application, GS needs to be applied in low LD populations which are found in many tree breeding programs. Here we propose an approach in which the significant markers from association studies may be used for developing prediction models in low LD populations using the same methods as in GS. Preliminary analyses indicate that a modest numbers of markers may be sufficient for developing prediction models in low LD populations. GS based on large numbers of random markers or small numbers of associated markers is poised to make marker-assisted selection a reality in forest tree breeding. 相似文献
7.
《遗传学报》2016,(4)
To effectively use elite genes on the long arm of rye chromosome 6(the 6RL arm) in wheat breeding programs,precise and fast identification of 6RL chromatin in wheat backgrounds is necessary.PCR-based 6RL-specific markers can facilitate the detection of elite genes on 6RL in wheat breeding.However,only a limited number of 6RL-specific markers have been developed.In the present study.300 new PCR-based 6RL-specific markers were identified using specific length amplified fragment sequencing(SLAF-seq) technology,and were further physically mapped to four regions on the 6RL arm using 6R and 6RL deletion lines.Interestingly,127 of the 300 markers were physically localized to a region from the site between 2.3 and 2.5 to the telomere,the same region where the powdery mildew resistance gene was mapped.In addition,95 of the 300 markers exhibit polymorphisms,which can be used to investigate the diversity of rye 6RL arms.The markers developed in this study can be used to identify given segments of 6RL in wheat backgrounds and accelerate the utilization of elite genes on 6RL in wheat breeding. 相似文献
8.
Genetic structures of the CIMMYT international yield trial targeted to irrigated environments 总被引:1,自引:0,他引:1
Susanne Dreisigacker Hailemichael Shewayrga Jose Crossa Vivi N. Arief Ian H. DeLacy Ravi P. Singh Mark J. Dieters Hans-Joachim Braun 《Molecular breeding : new strategies in plant improvement》2012,29(2):529-541
International yield trials are assembled by CIMMYT to disseminate promising wheat breeding materials worldwide. To assess
the genomic structure and linkage disequilibrium (LD) within this germplasm, wheat lines disseminated during 25 years of the
Elite Spring Wheat Yield Trial (ESWYT) targeted for irrigated environments of the world were genotyped with the high-throughput
Diversity Arrays Technology (DArT) marker system. Analyses of population structure assigned the ESWYT germplasm into five
major sub-populations that are shaped by prominent CIMMYT wheat lines and their descendants. Based on genetic distance, we
concluded that a constant level of genetic diversity was maintained over the years of ESWYT dissemination. Genetic distance
between the individual ESWYT lines significantly increased when the ESWYT were grouped according to the differences in years
of ESWYT dissemination, suggesting a systematic change in allele frequencies over time, most probably due to breeding and
directional selection. By means of multiple regression analyses, 78 markers displaying a significant change in allele frequency
across years were identified and interpreted as an indicator for constant selection. The markers identified were partly associated
with grain yield, leaf, stem, and yellow rust and point to key genomic regions for further investigation. Large numbers of
adjacent DArT marker pairs showed significant LD across the ESWYT population and within each of the five sub-populations identified.
Sub-population differentiation measured by the fixation index and average genetic distance were highly correlated with LD
levels, suggesting that the sub-populations themselves explain much of the LD. 相似文献
9.
Leaf rust is an important disease, threatening wheat production annually. Identification of resistance genes or QTLs for effective field resistance could greatly enhance our ability to breed durably resistant varieties. We applied a genome wide association study (GWAS) approach to identify resistance genes or QTLs in 338 spring wheat breeding lines from public and private sectors that were predominately developed in the Americas. A total of 46 QTLs were identified for field and seedling traits and approximately 20–30 confer field resistance in varying degrees. The 10 QTLs accounting for the most variation in field resistance explained 26–30% of the total variation (depending on traits: percent severity, coefficient of infection or response type). Similarly, the 10 QTLs accounting for most of the variation in seedling resistance to different races explained 24–34% of the variation, after correcting for population structure. Two potentially novel QTLs (QLr.umn-1AL, QLr.umn-4AS) were identified. Identification of novel genes or QTLs and validation of previously identified genes or QTLs for seedling and especially adult plant resistance will enhance understanding of leaf rust resistance and assist breeding for resistant wheat varieties. We also developed computer programs to automate field and seedling rust phenotype data conversions. This is the first GWAS study of leaf rust resistance in elite wheat breeding lines genotyped with high density 90K SNP arrays. 相似文献
10.
A genome-wide analysis of single nucleotide polymorphism diversity in the world's major cereal crops
Over 3.5 million expressed sequence tags from the major cereal taxa were used to electronically mine over 176 000 putative single nucleotide polymorphisms (SNPs). The density, distribution and degree of linkage between these SNPs were compared among the different taxa. The frequency of sequence polymorphism was lowest in diploid taxa (rice, barley and sorghum), intermediate in tetraploid maize and highest in allohexaploid wheat and octoploid sugarcane. SNPs were further categorized as either intravarietal (differences between gene family members and homoeologues) or varietal (differences between two varieties), and as either co-segregating or non-co-segregating with neighbouring polymorphisms. Varietal co-segregating SNPs represent the best candidates for molecular markers as they show variation between varieties and have a high probability of being validated, as sequencing errors are unlikely to co-segregate with one another. This elite class of SNPs was most abundant in barley and least abundant in wheat and rice. Despite the large number of observed sequence polymorphisms in allohexaploid wheat, only a fraction of those available are likely to make good molecular markers. In addition, we found that rice SNPs up to 10 kb apart were in linkage disequilibrium (LD), but that high levels of LD attributable to population structure confounded the tracking of LD over greater distances. 相似文献
11.
Hasina Begum Muhammad Shahedul Alam Yanru Feng Patrice Koua Md Ashrafuzzaman Asis Shrestha Mohammad Kamruzzaman Said Dadshani Agim Ballvora Ali Ahmad Naz Michael Frei 《Plant, cell & environment》2020,43(11):2650-2665
Rising tropospheric ozone affects the performance of important cereal crops thus threatening global food security. In this study, genetic variation of wheat regarding its physiological and yield responses to ozone was explored by exposing a diversity panel of 150 wheat genotypes to elevated ozone and control conditions throughout the growing season. Differential responses to ozone were observed for foliar symptom formation quantified as leaf bronzing score (LBS), vegetation indices and yield components. Vegetation indices representing the carotenoid to chlorophyll pigment ratio (such as Lic2) were particularly ozone-responsive and were thus considered suitable for the non-invasive diagnosing of ozone stress. Genetic variation in ozone-responsive traits was dissected by a genome-wide association study (GWAS). Significant marker-trait associations were identified for LBS on chromosome 5A and for vegetation indices (NDVI and Lic2) on chromosomes 6B and 6D. Analysis of linkage disequilibrium (LD) in these chromosomal regions revealed distinct LD blocks containing genes with a putative function in plant redox biology such as cytochrome P450 proteins and peroxidases. This study gives novel insight into the natural genetic variation in wheat ozone response, and lays the foundation for the molecular breeding of tolerant wheat varieties. 相似文献
12.
Patterns of diversity and linkage disequilibrium within the cosmopolitan inversion In(3R)Payne in Drosophila melanogaster are indicative of coadaptation
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The cosmopolitan inversion In(3R)Payne in Drosophila melanogaster decreases in frequency with increasing distance from the equator on three continents, indicating it is subject to strong natural selection. We investigated patterns of genetic variation and linkage disequilibrium (LD) in 24 molecular markers located within and near In(3R)Payne to determine if different parts of the inversion responded to selection the same way. We found reduced variation in the markers we used compared to others distributed throughout the genome, consistent with the inversion having a relatively recent origin (相似文献
13.
DOES LINKAGE DISEQUILIBRIUM GENERATE HETEROZYGOSITY‐FITNESS CORRELATIONS IN GREAT REED WARBLERS? 总被引:9,自引:0,他引:9
Hansson B Westerdahl H Hasselquist D Akesson M Bensch S 《Evolution; international journal of organic evolution》2004,58(4):870-879
Heterozygosity-fitness correlations (HFCs) at noncoding genetic markers are commonly assumed to reflect fitness effects of heterozygosity at genomewide distributed genes in partially inbred populations. However, in populations with much linkage disequilibrium (LD), HFCs may arise also as a consequence of selection on fitness loci in the local chromosomal vicinity of the markers. Recent data suggest that relatively high levels of LD may prevail in many ecological situations. Consequently, LD may be an important factor, together with partial inbreeding, in causing HFCs in natural populations. In the present study, we evaluate whether LD can generate HFCs in a small and newly founded population of great reed warblers (Acrocephalus arundinaceus). For this purpose dyads of full siblings of which only one individual survived to adult age (i.e., returned to breed at the study area) were scored at 19 microsatellite loci, and at a gene region of hypothesized importance for survival, the major histocompatibility complex (MHC). By examining siblings, we controlled for variation in the inbreeding coefficient and thus excluded genome-wide fitness effects in our analyses. We found that recruited individuals had significantly higher multilocus heterozygosity (MLH), and mean d2 (a microsatellite-specific variable), than their nonrecruited siblings. There was a tendency for the survivors to have a more diverse MHC than the nonsurvivors. Single-locus analyses showed that the strength of the genotype-survival association was especially pronounced at four microsatellite loci. By using genotype data from the entire breeding population, we detected significant LD between five of 162 pairs of microsatellite loci after accounting for multiple tests. Our present finding of a significant within-family multilocus heterozygosity-survival association in a nonequilibrium population supports the view that LD generates HFCs in natural populations. 相似文献
14.
Population structure, genetic diversity and linkage disequilibrium in elite winter wheat assessed with SNP and SSR markers 总被引:3,自引:0,他引:3
Tobias Würschum Simon M. Langer C. Friedrich H. Longin Viktor Korzun Eduard Akhunov Erhard Ebmeyer Ralf Schachschneider Johannes Schacht Ebrahim Kazman Jochen C. Reif 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(6):1477-1486
Modern genomics approaches rely on the availability of high-throughput and high-density genotyping platforms. A major breakthrough in wheat genotyping was the development of an SNP array. In this study, we used a diverse panel of 172 elite European winter wheat lines to evaluate the utility of the SNP array for genomic analyses in wheat germplasm derived from breeding programs. We investigated population structure and genetic relatedness and found that the results obtained with SNP and SSR markers differ. This suggests that additional research is required to determine the optimum approach for the investigation of population structure and kinship. Our analysis of linkage disequilibrium (LD) showed that LD decays within approximately 5–10 cM. Moreover, we found that LD is variable along chromosomes. Our results suggest that the number of SNPs needs to be increased further to obtain a higher coverage of the chromosomes. Taken together, SNPs can be a valuable tool for genomics approaches and for a knowledge-based improvement of wheat. 相似文献
15.
Manuela Diethelm Michael Schmolke Jennifer Groth Wolfgang Friedt Günther Schweizer Lorenz Hartl 《Molecular breeding : new strategies in plant improvement》2014,34(1):31-43
Fusarium head blight (FHB) is a destructive disease of wheat and barley. In wheat it is mainly caused by the fungal pathogens Fusarium graminearum and Fusarium culmorum. We report the identification and evaluation of candidate genes for quantitative FHB resistance. These genes showed altered expression levels in the moderately resistant winter wheat genotypes Capo and SVP72017 after inoculation with F. graminearum. Amongst others, a NPR1-like gene was identified. Sequence analysis of this gene fragment revealed a high level of variation between the parents of a doubled haploid population. Single nucleotide polymorphism and polymerase chain reaction markers were developed and two homoeologous genes were mapped on the long arms of chromosomes 2A and 2D, respectively. Markers for both genes had significant effects on FHB resistance in a diverse collection of 178 European winter wheat cultivars evaluated in multi-environmental field trials after spray inoculation with F. culmorum. These results revealed that allelic variation in two homoeologous NPR1-like genes is associated with FHB resistance in European winter wheat. Markers for these genes might therefore be used for marker-assisted breeding programs. 相似文献
16.
小麦改良的可利用资源:黑麦抗病基因 总被引:3,自引:0,他引:3
黑麦(Secale cereale)蕴藏着丰富的抗病基因,是改良小麦抗性的重要资源,黑麦抗病基因的导入一直是小麦育种的重要研究课题。本文综述了黑麦抗病基因的染色体定位、分子标记研究和含黑麦抗病基因的小麦种质资源在我国小麦育种中的应用,对应用中存在的问题进行了分析,并对今后的研究方向进行了展望。 相似文献
17.
Hanan Sela Smadar Ezrati Pnina Ben-Yehuda Jacob Manisterski Eduard Akhunov Jan Dvorak Adina Breiman Abraham Korol 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2014,127(11):2453-2463
Key Message
Rapid LD decay in wild emmer population from Israel allows high-resolution association mapping. Known and putative new stripe rust resistance genes were found.Abstract
Genome-wide association mapping (GWAM) is becoming an important tool for the discovery and mapping of loci underlying trait variation in crops, but in the wild relatives of crops the use of GWAM has been limited. Critical factors for the use of GWAM are the levels of linkage disequilibrium (LD) and genetic diversity in mapped populations, particularly in those of self-pollinating species. Here, we report LD estimation in a population of 128 accessions of self-pollinating wild emmer, Triticum turgidum ssp. dicoccoides, the progenitor of cultivated wheat, collected in Israel. LD decayed fast along wild emmer chromosomes and reached the background level within 1 cM. We employed GWAM for the discovery and mapping of genes for resistance to three isolates of Puccinia striiformis, the causative agent of wheat stripe rust. The wild emmer population was genotyped with the wheat iSelect assay including 8643 gene-associated SNP markers (wheat 9K Infinium) of which 2,278 were polymorphic. The significance of association between stripe rust resistance and each of the polymorphic SNP was tested using mixed linear model implemented in EMMA software. The model produced satisfactory results and uncovered four significant associations on chromosome arms 1BS, 1BL and 3AL. The locus on 1BS was located in a region known to contain stripe rust resistance genes. These results show that GWAM is an effective strategy for gene discovery and mapping in wild emmer that will accelerate the utilization of this genetic resource in wheat breeding. 相似文献18.
Rosy Raman Harsh Raman Peter Martin 《Molecular breeding : new strategies in plant improvement》2007,19(4):315-328
Higher polyphenol oxidase (PPO) activity in wheat kernels and flour has been implicated in the time dependent darkening of
various end-products. Previous study conducted on a bread wheat (Triticum aestivum L.) doubled haploid (DH) mapping population derived from Chara (medium-high PPO) and WW2449 (low PPO) identified a major
QTL for PPO activity located on the long arm of chromosome 2A. Physical mapping of SSR markers accounting for up to 84% of
phenotypic variation for PPO activities suggests that the candidate PPO locus is localised in the deletion bin delimited by
2AL 0.77–0.85. In order to develop functional gene markers, nine wheat ESTs mapped to this deletion bin and partial PPO reference
genes were explored for their sequence identities and linkage with PPO locus in a mapping population. In the present study,
two markers: one SNP and one CAPS based upon BQ161439 sequence variation between the parents were identified which exhibited
a tight linkage (0–0.6 cM) with the PPO loci designated as XTc1 and XPPO-
LDOPA. We also mapped the reference PPO gene (GenBank AY526268) characterised from developing kernels of wheat, on the long
arm of chromosome 2A which exhibited a complete linkage with XPPO-
L
DOPA locus. Results suggest that PPO variation displayed in the DH population from Chara/WW2449 is due to the same reference PPO
gene. Allelic homoplasy of tightly linked markers, indicated that these markers are ‘diagnostic’ for the selection of low
PPO gene in a range of germplasm being used in different Australian breeding programs. Identification and validation of ‘functional
gene markers’ would facilitate in enhancing the selection efficiency for low PPO activity in wheat breeding programs. 相似文献
19.
小麦赤霉病严重威胁我国粮食和食品安全,培育抗赤霉病小麦品种是解决该病害最经济有效的途径。20世纪90年代后,以扬麦158为代表的扬麦、宁麦系列中抗赤霉病品种的育成和大面积推广有效抵御了长江中下游麦区的赤霉病危害,使我国抗赤霉病育种处于国际领先水平。尽管全球明确了7个抗赤霉病基因,为开展抗赤霉病育种提供了重要支撑,但由于赤霉病抗性机制复杂,实现高抗与高产的协调仍极其困难,抗赤霉病仍是当前及未来我国小麦育种的主要目标。对“十三五”期间我国小麦新品系和审定品种的抗性情况以及我国抗赤霉病育种方面取得的进展进行了综述,并提出了重视挖掘和利用扬麦等推广品种中优异抗性基因、将Fhb1导入扬麦等主栽品种的育种技术路线和重视表型精准鉴定等建议,以期为实现我国抗赤霉病育种突破提供借鉴。 相似文献