Comparative study of water uptake and photosynthetic gas exchange between scrub and fringe red mangroves,Rhizophora mangle L.

Summary The red mangrove (Rhizophora mangle L.) occurs frequently in both scrub and fringe mangrove forests. Our previous study demonstrated that individuals of this mangrove species growing in scrub and fringe forests differ significantly in both morphological and physiological characteristics. To further characterize physiological differences between scrub and fringe mangroves, we compared their differences in water uptake and photosynthetic gas exchange during different seasons. In the wet season (June–October, 1990), scrub mangroves showed lower D and ¹⁸O values of stem water than fringe mangroves, indicating more usage of rain-derived freshwater. In the dry season (Jan–April, 1991), however, scrub mangroves utilized the same water source as fringe mangroves, reflected by their similar D and ¹⁸O values of stem water. Consistently, there were significant differences in predawn water potentials between scrub and fringe mangroves in the wet season (October 1990) with higher values for scrub mangroves, but no significant differences in the dry season (January 1991). Higher elevation in the scrub forest seems to be the major factor responsible for the shift of water sources in scrub mangroves. On Apr. 27 and Aug. 8, 1990, scrub mangroves showed lower CO₂ assimilation rate, stomatal conductance, and intercellular CO₂ concentration than fringe mangroves. There were no differences in these gas exchange characteristics on the other two measuring dates: Oct. 17, 1990 and Jan. 11, 1991. Instantaneous water use efficiency was significantly higher for scrub mangroves than for fringe mangroves on three of the four sampling dates. Similarly, leaf carbon isotope discrimination of scrub mangroves was always significantly lower than that of fringe mangroves, indicating higher long-term water use efficiency. Higher water use efficiency in scrub mangroves is a result of stomatal limitation on photosynthesis, which may entail considerable carbon cost to the plants.     

Emissions of carbon from forestry and land-use change in tropical Asia

The net emissions of carbon from forestry and changes in land use in south and southeast Asia were calculated here with a book-keeping model that used rates of land-use change and associated per hectare changes in vegetation and soil to calculate changes in the amount of carbon held in terrestrial ecosystems and wood products. The total release of carbon to the atmosphere over the period 1850–1995 was 43.5 PgC. The clearing of forests for permanent croplands released 33.5 PgC, about 75% of the total. The reduction of biomass in the remaining forests, as a result of shifting cultivation, logging, fuelwood extraction, and associated regrowth, was responsible for a net loss of 11.5 PgC, and the establishment of plantations withdrew from the atmosphere 1.5 PgC, most of it since 1980. Based on comparisons with other estimates, the uncertainty of this long-term flux is estimated to be within ±30%. Reducing this uncertainty will be difficult because of the difficulty of documenting the biomass of forests in existence >40 years ago. For the 15-y period 1981–1995, annual emissions averaged 1.07 PgC y^–1, about 50% higher than reported for the 1980s in an earlier study. The uncertainty of recent emissions is probably within ± 50% but could be reduced significantly with systematic use of satellite data on changes in forest area. In tropical Asia, the emissions of carbon from land-use change in the 1980s accounted for approximately 75% of the region’s total carbon emissions. Since 1990 rates of deforestation and their associated emissions have declined, while emissions of carbon from combustion of fossil fuels have increased. The net effect has been a reduction in emissions of CO₂ from this region since 1990.     

δ13C signature of organic carbon in estuarine bottom sediment as an indicator of carbon export from adjacent marshes

The ¹³C signature of organic carbon in estuarine bottom sediment in Louisiana Barataria Basin was used for estimating carbon flux from adjacent marsh. The stable carbon isotope composition of plants, soils and sediments from the basin were determined. The ¹³C content of marsh vegetation ranged from -26.3 to -27.8% for C₃ freshwater vegetation in the upper basin to -13.0 to -13.3% for C₄ vegetation in the lower basin. The ¹³C content of the highly organic marsh soils were similar to ¹³C content of vegetation present. The ¹³C content of organic carbon from bottom sediment of open water bodies ranged from 27.3 in the upper basin (freshwater) to 16.4 in bottom sediment of salt marsh ponds. The¹³C signature of organic carbon in bottom sediment from saline regions corresponded to the size of the body of water. The smaller salt marsh ponds contain sediment with ¹³C values close to that of the C₄ plantSpartina alterniflora. Results suggest that phytoplankton rather thanSpartina alterniffora is the likely organic source in bottom sediment of the larger bay near the coast (e.g. Caminada Bay).     

Biomass change in an Atlantic tropical moist forest: the ENSO effect in permanent sample plots over a 22-year period

There are a number of controversies surrounding both biomass estimation and carbon balance in tropical forests. Here we use long-term (from 1978 through 2000) data from five 0.5-ha permanent sample plots (PSPs) within a large tract of relatively undisturbed Atlantic moist forest in southeastern Brazil to quantify the biomass increment (M_I), and change in total stand biomass (M_stand), from mortality, recruitment, and growth data for trees 10 cm diameter at breast height (DBH). Despite receiving an average of only 1,200 mm annual precipitation, total forests biomass (334.5±11.3 Mg ha^–1) was comparable to moist tropical forests with much greater precipitation. Over this relatively long-term study, forest biomass experienced rapid declines associated with El Niño events, followed by gradual biomass accumulation. Over short time intervals that overlook extreme events, these dynamics can be misinterpreted as net biomass accumulation. However for the 22 years of this study, there was a small reduction in forest biomass, averaging –1.2 Mg ha^–1 year^–1 (±3.1). Strong climatic disturbances can severely reduce forest biomass, and if the frequency and intensity of these events increases beyond historical averages, these changing disturbance regimes have the capacity to significantly reduce forest biomass, resulting in a net source of carbon to the atmosphere.     

Environmental change and the carbon balance of Amazonian forests

Extreme climatic events and land‐use change are known to influence strongly the current carbon cycle of Amazonia, and have the potential to cause significant global climate impacts. This review intends to evaluate the effects of both climate and anthropogenic perturbations on the carbon balance of the Brazilian Amazon and to understand how they interact with each other. By analysing the outputs of the Intergovernmental Panel for Climate Change (IPCC) Assessment Report 4 (AR4) model ensemble, we demonstrate that Amazonian temperatures and water stress are both likely to increase over the 21st Century. Curbing deforestation in the Brazilian Amazon by 62% in 2010 relative to the 1990s mean decreased the Brazilian Amazon's deforestation contribution to global land use carbon emissions from 17% in the 1990s and early 2000s to 9% by 2010. Carbon sources in Amazonia are likely to be dominated by climatic impacts allied with forest fires (48.3% relative contribution) during extreme droughts. The current net carbon sink (net biome productivity, NBP) of +0.16 (ranging from +0.11 to +0.21) Pg C year^?1 in the Brazilian Amazon, equivalent to 13.3% of global carbon emissions from land‐use change for 2008, can be negated or reversed during drought years [NBP = ?0.06 (?0.31 to +0.01) Pg C year^?1]. Therefore, reducing forest fires, in addition to reducing deforestation, would be an important measure for minimizing future emissions. Conversely, doubling the current area of secondary forests and avoiding additional removal of primary forests would help the Amazonian gross forest sink to offset approximately 42% of global land‐use change emissions. We conclude that a few strategic environmental policy measures are likely to strengthen the Amazonian net carbon sink with global implications. Moreover, these actions could increase the resilience of the net carbon sink to future increases in drought frequency.     

Influence of Earthworm Invasion on Redistribution and Retention of Soil Carbon and Nitrogen in Northern Temperate Forests

We analyzed soil organic matter distribution and soil solution chemistry in plots with and without earthworms at two sugar maple (Acer saccharum)–dominated forests in New York State, USA, with differing land-use histories to assess the influence of earthworm invasion on the retention or loss of soil carbon (C) and nitrogen (N) in northern temperate forests. Our objectives were to assess the influence of exotic earthworm invasion on (a) the amount and depth distribution of soil C and N, (b) soil ¹³C and ¹⁵N, and (c) soil solution chemistry and leaching of C and N in forests with different land-use histories. At a relatively undisturbed forest site (Arnot Forest), earthworms eliminated the thick forest floor, decreased soil C storage in the upper 12 cm by 28%, and reduced soil C:N ratios from 19.2 to 15.3. At a previously cultivated forest site with little forest floor (Tompkins Farm), earthworms did not influence the storage of soil C or N or soil C:N ratios. Earthworms altered the stable isotopic signature of soil at Arnot Forest but not at Tompkins Farm; the alteration of stable isotopes indicated that earthworms significantly increased the loss of forest floor C but not N from the soil profile at Arnot Forest. Nitrate (NO₃^–) concentrations in tension and zero-tension lysimeters were much greater at Tompkins Farm than Arnot Forest, and earthworms increased NO₃^– leaching at Tompkins Farm. The results suggest that the effect of earthworm invasion on the distribution, retention, and solution chemistry of soil C and N in northern temperate forests may depend on the initial quantity and quality of soil organic matter at invaded sites.     

Carbon and nitrogen cycling in intertidal sediments near Doel,Scheldt Estuary

Carbon and nitrogen cycling in intertidal mud flat sediments in the Scheldt Estuary was studied using measurements of carbon dioxide, methane and nitrous oxide emission rates and pore-water profiles of CO₂, ammonium and nitrate. A comparison between chamber measured carbon dioxide fluxes and those based on CO₂ pore-water gradients using Fick's First law indicates that apparent diffusion coefficients are 2 to 28 times higher than bulk sediment diffusion coefficients based on molecular diffusion. Seasonal changes in gaseous carbon fluxes or CO₂ pore water concentrations cannot be used directly, or in a simple way, to determine seasonal rates of mineralization, because of marked seasonal changes in pore-water storage and exchange parameters.The annual amount of carbon delivered to the sediment is 42 mol m^–2, of which about 42% becomes buried, the remaining being emitted as methane (7%) or carbon dioxide (50%). Each year about 2.6 mol N m^–2 of particulate nitrogen reaches the sediment; 1.1 mol m^–2 is buried and 1.6 mol m^–2 is mineralized to ammonium. Only 0.42 mol m^–2 yr^–1 of the ammonium produced escapes from the sediments, the remaining being first nitrified (1.2 mol m^–2 yr^–1) and then denitrified (1.7 mol m^–2 yr^–1). Simple calculations indicate that intertidal sediments may account for about 14% and 30% of the total estuarine retention of nitrogen and carbon, respectively.     

Mind Architecture and Brain Architecture

The use of the computer metaphor has led to the proposal of mind architecture (Pylyshyn 1984; Newell 1990) as a model of the organization of the mind. The dualist computational model, however, has, since the earliest days of psychological functionalism, required that the concepts mind architecture and brain architecture be remote from each other. The development of both connectionism and neurocomputational science, has sought to dispense with this dualism and provide general models of consciousness – a uniform cognitive architecture –, which is in general reductionist, but which retains the computer metaphor. This paper examines, in the first place, the concepts of mind architecture and brain architecture, in order to evaluate the syntheses which have recently been offered. It then moves on to show how modifications which have been made to classical functionalist mind architectures, with the aim of making them compatible with brain architectures, are unable to resolve some of the most serious problems of functionalism. Some suggestions are given as to why it is not possible to relate mind structures and brain structures by using neurocomputational approaches, and finally the question is raised of the validity of reductionism in a theory which sets out to unite mind and brain architectures.     

基于土地利用变化的四川省碳排放与碳足迹效应及时空格局

土地利用变化的碳排放与碳足迹研究对了解人类活动对生态环境的扰动程度及其机理、制定有效的碳排放政策具有重要意义。采用1990—2010年四川省能源消费数据和土地利用数据,通过构建碳排放模型、碳足迹及其压力指数模型,对研究区20年来土地利用的碳排放及碳足迹进行了定量分析。结果表明:(1)土地利用变化的碳排放和能源消费碳的足迹呈显著增加趋势。碳排放增加5407.839×10~4t,增长率达143%;能源消费的碳足迹增加1566.622×10~4hm~2,四川全省的生态赤字达1563.598×10~4hm~2。(2)建设用地和林地分别为四川省最大的碳源与碳汇。20年间建设用地的碳排放增加5407.072×10~4t,增长率达126.27%,占碳排放总量的88%以上;林地的碳汇减少10.351×10~4t,但仍占四川省碳汇的96%以上。(3)土地利用碳排放、碳足迹和生态赤字存在明显区域差异。成都平原区碳排放、碳足迹压力最大,生态赤字严重,西部高山高原区和盆周山区碳排放、碳足迹最小,未出现生态赤字;成都、德阳、资阳和内江等地的碳排放、碳足迹压力最大,生态赤字最严重,甘孜、阿坝等地的碳排放、碳足迹最小,未出现生态赤字。(4)土地利用结构与碳排放、碳足迹存在一定的相互关系,趋高的碳源、碳汇比导致土地利用的碳源效应远大于碳汇效应。因此,四川省减排的重点应该在保持或增加现有的林地的同时,主要以降低建设用地的碳排放、碳足迹为主。     

Sources of organic carbon in mangrove sediments: variability and possible ecological implications

Mangrove sediments from three different mangrove ecosystems (Coringa Wildlife Sanctuary in the Godavari Delta, Andhra Pradesh, India, and Galle and Pambala, south-west Sri Lanka) were analysed for their organic carbon content, elemental ratios (C:N) and carbon stable isotope composition. Organic carbon content (0.6 – 31.7% dry weight), C/N ratios (7.0 – 27.3) and ¹³C (between –29.4 and –20.6) showed a wide range of values. Lower stocks of organic carbon coincided with low C/N (atom) ratios and less negative ¹³C values, indicating import of marine or estuarine particulate suspended matter. High organic carbon stocks coincided with high C/N ratios and ¹³C values close, but not equal, to those of the mangrove vegetation. The variations observed in this study and published literature data could be adequately described by a simple two-end mixing model, whereby marine/estuarine suspended matter and mangrove litter were taken as end members. Thus, while in some mangrove ecosystems or vegetation zones, organic carbon stocks can be very high and are almost entirely of mangrove origin, there also appear to be cases in which deposited estuarine or marine suspended matter is the dominant source of organic carbon and nitrogen in mangrove sediments. This situation is remarkably similar to that observed in temperate salt marsh ecosystems where the importance of local vascular plant production to the sediment organic carbon pool is equally variable. The observed high variability in organic matter origin is thought to have a major impact on the overall carbon dynamics in intertidal mangrove ecosystems.     

Alpha-thalassemia in Papua New Guinea

Summary A study of the distribution of -thalassemia in Papua New Guinea (PNG) was carried out by DNA analysis. A total of 664 DNA samples were screened for -thalassemia 2 and -thalassemia 1 caused respectively by either deletion of one or both of the duplicated -globin genes. -Thalassemia 2 was detected in high frequencies in coastal and lowland regions where malaria has been holo- to hyperendemic but in low frequencies in non-malarious highland regions. The highest frequency was observed in the north coast of PNG. The distribution of -thalassemia 2 seems to be in accordance with other conditions such as ovalocytosis and G6PD deficiency which are also prevalent in this population, suggesting that they may interact in protection against malaria. However, it appears to be negatively correlated with -thalassemia and -thalassemia 1, the latter being extremely rare in this population. Analysis of the types and subtypes of the single -globin gene deletion revealed a predominance of the –^4.2 type in general, except in some regions in the south where the –^3.7 type is prevalent. The –^3.7 I subtype is the common form of the –^3.7 deletion in the PNG mainland. The –^3.7 III subtype, previously reported to be unique in Melanesians and Polynesians, was detected in an offshore island of PNG. However, this subtype is very rare in Melanesians from the PNG mainland.     

Adsorption of small biological molecules on silica from diluted aqueous solutions: Quantitative characterization and implications to the Bernal's hypothesis

To describe quantitatively the adsorption of prebiotically important compounds of low molecular weight (amino acids, short linear peptides, cyclic dipeptides, the Krebs's cycle and other carboxylic acids, nucleosides and related phosphates) on silica surface from diluted neutral aqueous solutions, equilibrium constants (K) and free energies (–G) of adsorption were determined from the retention values measured by means of high-performance liquid chromatography on a silica gel column and from the isotherms measured under static conditions. For most carboxylic acids (including amino acids and linear peptides) –G values were negative and K<1, thus showing very weak adsorption. Cyclic dipeptides (2,5-piperazinediones) exhibited higher adsorbability; –G>0 and K>1 were found for most of them. Influence of the structure of -substituent on the adsorbability is analyzed. A linear dependence of –G on the number of aliphatic carbon atoms in a sorbate molecule was found for the series of aliphatic bifunctional amino acids, related dipeptides and 2,5-piperazinediones, as well as for the row from glycine to triglycyl glycine. The adsorption of nucleosides and their phosphates is characterized by much higherK and –G values (of the order of 10² and 10⁴, respectively). The adsorption data available from our work and literature are summarized and discussed with implications to the Bernal's hypothesis on the roles of solid surfaces in the prebiotic formation of biopolymers from monomeric building blocks.corresponding author: on leave of absence from Institute of Surface Chemistry, Academy of Sciences of the Ukraine, Kiev, Ukraine.     

Analysis of some regular systems of inbreeding

Summary A probabilistic method is used to calculate the asymptotic rate of loss of genetic variability for half-sib and first-cousin mating of an infinite number of individuals. For both systems of inbreeding, all probabilities of nonidentity by descent converge to zero at ultimate rates proportional to t ^–1/2, where t is the time in generations. The rate of decay is faster for half-sibs than for first cousins, the respective asymptotic allozygosities being 4(t)^–1/2 and 8(t)^–1/2.Supported by the National Science Foundation (Grant No. DEB77-21494).     

Dose-dependent nonlinear response of the main phase-transition temperature of phospholipid membranes to alcohols

Summary The effect of 1-alkanols upon the main phase-transition temperature of phospholipid vesicle membranes between gel and liquid-crystalline phases was not a simple monotonic function of alkanol concentration. For instance, 1-decanol decreased the transition temperature at low concentrations, but increased it at high concentrations, displaying a minimal temperature. This concentration-induced biphasic effect cannot be explained by the van't Hoff model on the effect of impurities upon the freezing point. To explain this nonlinear response, a theory is presented which treats the effect of 1-alkanols (or any additives) on the transition temperature of phospholipid membranes in a three-component mixture. By fitting the experimental data to the theory, the enthalpy of the phase transition H ^* and the interaction energy, _AB ^* between the additive and phospholipid molecules may be estimated. The theory predicts that when _AB ^* >2 (where _AB ^* = _AB,/RT _o,T _o being the transition temperature of phospholipid), both maximum and maximum transition temperatures should exist. When _AB ^* = 2, only one inflection point exists. When _AB ^* < 2, neither maximum nor minimum exists. The alkanol concentration at which the transition temperature is minimum (X _min) depends on the _AB ^* value: the larger the _AB ^* values, the smaller theX _min. When _AB ^* is large enough,X _min values become so small that the plot T vs.X shows positive T in almost all alkanol concentrations. The interaction energy between 1-alkanols and phospholipid molecules increased with the increase in the carbon chain-length of 1-alkanols. In the case of the dipalmitcylphosphatidylcholine vesicle membrane, the carbon chain-length of 1-alkanols that caused predominantly positive T was about 12.     

Net Carbon Emissions from Deforestation in Bolivia during 1990-2000 and 2000-2010: Results from a Carbon Bookkeeping Model

Accurate estimates of global carbon emissions are critical for understanding global warming. This paper estimates net carbon emissions from land use change in Bolivia during the periods 1990–2000 and 2000–2010 using a model that takes into account deforestation, forest degradation, forest regrowth, gradual carbon decomposition and accumulation, as well as heterogeneity in both above ground and below ground carbon contents at the 10 by 10 km grid level. The approach permits detailed maps of net emissions by region and type of land cover. We estimate that net CO₂ emissions from land use change in Bolivia increased from about 65 million tons per year during 1990–2000 to about 93 million tons per year during 2000–2010, while CO₂ emissions per capita and per unit of GDP have remained fairly stable over the sample period. If we allow for estimated biomass increases in mature forests, net CO₂ emissions drop to close to zero. Finally, we find these results are robust to alternative methods of calculating emissions.     

Cultivation of the agarophyte Gelidium pristoides in Algoa Bay,South Africa

Biofilms were allowed to develop on wooden slides of the River Red Gum (Eucalyptus camaldulensis Dehnh., Myrtaceae) submerged in two billabongs of south-eastern Australia. The slides were placed in the photic zone and the aphotic zone, and the biofilms sampled after eight week's growth over the summer of 1989–1990 and winter of 1990. Bacterial numbers, estimated with epifluorescence microscopy, ranged from 4–78 × 10⁶ cells cm^–2. Bacteria were more abundant in the photic zone than the aphotic zone, and more abundant in summer than winter. Fewer than 0.5% of the bacteria could be cultivated on nutrient agar plates. Concentrations of phospholipids ranged from 8–79 ng cm^–2, which corresponded to bacterial abundances of 2–17 × 10⁶ cells cm^–2. Fifty five phospholipid fatty acids (PLFA) were identified, of which 16:0 (13–29% of total PFLA) was the most common. Other abundant PFLA included 16:17c (6–28%), 18:26 (3–16%), 18:33 (4–12%), 18:19c (3–5%), 18:l7c (5–11%) and 18:0 (2–8%). Minor PLFA included 14:0, i and a 15:0, 15:0, 16:l5c, 16:113c, 18:36, 18:43, 20:46 and 20:53. The PLFA profiles of the biofilms were quite different from those of the sediments and plankton. There was a clear distinction between the PLFA profiles of summer and winter biofilms, but less evidence for unequivocal site or light-regime effects.     

湖南省森林植被碳储量、碳密度动态特征

利用湖南省4次(1983—1987年、1990—1995年、2003—2004年和2009年)森林资源清查数据,采用材积源-生物量法,结合湖南省现有森林植被主要树种碳含量实测数据,研究近20多年来湖南省森林植被碳储量、碳密度的动态特征。结果表明:从1987年到2009年,湖南省乔木林植被碳汇为66.40×106tC,碳密度提高了5.65 tC/hm~2,阔叶林碳汇最大(48.43×10~6tC),其次是杉木林(9.54×10~6tC)和松木林(6.68×10~6tC),各乔木林植被碳密度波动较大;除过熟林外,各龄组乔木林均为碳汇,中龄林碳汇最大,幼龄林、中龄林、近熟林植被碳密度依次提高了4.75、4.09、0.83 tC/hm~2,成熟林、过熟林分别下降了6.87、13.88 tC/hm~2;天然林、人工林植被碳汇分别为41.01×10~6tC、25.39×10~6tC,碳密度分别提高了7.19、4.91 tC/hm~2。湖南省森林植被(包括疏林)碳汇为84.87×10~6tC,乔木林碳汇最大,其次是竹林,分别占湖南省森林植被碳汇的78.24%和33.31%,碳密度提高了6.24 tC/hm~2,各森林类型植被碳储量随其面积变化而变化。表明近20多年来,湖南省乔木林植被单位面积储碳能力明显提高,天然林在湖南省乔木林植被碳储量占有重要地位。     

Changes in terrestrial carbon storage in the United States. 1: The roles of agriculture and forestry

• 1 Changes in the areas of croplands and pastures, and rates of wood harvest in seven regions of the United States, including Alaska, were derived from historical statistics for the period 1700–1990. These rates of land‐use change were used in a cohort model, together with equations defining the changes in live vegetation, slash, wood products and soil that follow a change in land use, to calculate the annual flux of carbon to the atmosphere from changes in land use.
• 2 The calculated flux increased from less than 10 TgC/yr in 1700 to a maximum of about 400 TgC/yr around 1880 and then decreased to approximately zero by 1950. The total flux for the 290‐year period was a release of 32.6 PgC. The area of forests and woodlands declined by 42% (160 × 10⁶ ha), releasing 29 PgC, or 90% of the total flux. Cultivation of soils accounted for about 25% of the carbon loss. Between 1950 and 1990 the annual flux of carbon was approximately zero, although eastern forests were accumulating carbon.
• 3 When the effects of fire and fire exclusion (reported in a companion paper) were added to this analysis of land‐use change, the uptake of carbon calculated for forests was similar in magnitude to the uptake measured in forest inventories, suggesting that past harvests account for a significant fraction of the observed carbon sink in forests.
• 4 Changes in the management of croplands between 1965 and 1990 may have led to an additional accumulation of carbon, not included in the 32.6 PgC release, but even with this additional non‐forest sink, the calculated accumulation of carbon in the United States was an order of magnitude smaller than the North American carbon sink inferred recently from atmospheric data and models.

     

Mechanism of B12-dependent ribonucleotide reductase

Summary Ribonucleotide reductase from L. leichmmannii catalyzes cleavage of the carbon cobalt bond of AdoCbl homolytically in a kinetically competent fashion. This cleavage triggers a chain of events which results in cleavage of the 3C-H bond of the nucleotide substrate followed by cleavage of the 2 carbon hydroxyl bond. Involvement of a radical cation has been suggested as a possible mechanism by which this unusual reduction reaction might occur. Furthermore, cleavage of the 3 carbon hydrogen bond of [3-³H]NTP resulted in no ³H release to solvent and no ³H recovered in AdoCbl. These results were interpreted to mean that in this system AdoCbl does not serve as a H abstractor, but rather as a radical chain initiator. A protein residue on the RTPR is postulated to carry out the actual H abstraction from the substrate.These results and the conclusions drawn from them are further supported by recent experiments using [3-³H]CIUTP. Incubation of RTPR with [3-³H]CIUTP resulted in release of ³H₂O, uracil, PPP_i, formation of Coll and 5 deoxyadenosine. The ³H₂O release confirms the enzyme's ability to cleave the 3C-H bond of a nucleotide analog. Furthermore, little if any ³H was recovered in the 5 deoxyadenosine and the rate of ³H₂O release from [3³H]CIUTP was 12 times faster than the rate of ³H₂O release from [5-³H]AdoCbl. These observations support the conclusions drawn from data with the normal substrate; ie, AdoCbl serves as a radical chain initiator rather than a direct H abstractor from substrate.     

Carbon isotopes and carbon turnover in cotton and wheat FACE experiments

The Maricopa cotton and wheat FACE (free-air CO₂ enrichment) experiments offer propitious opportunity to quantify carbon turnover. The commercial CO₂ (% MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaeqiTdq2aaW% baaSqabeaacaaIXaGaaG4maaaakiaaboeacqGHijYUcqGHsislcaaI% ZaGaaG4naiaacwcaliaad+gaaaa!3FCB!\[\delta ^{13} {\text{C}} \approx - 37\% o\]) used to elevate CO₂ concentration in field plots provided a strongly ¹³C-depleted tracer. Soil CO₂ and ¹³C of soil organic carbon (SOC) in CO₂-enriched and Control plots were measured between the final cotton FACE project (October 1991) and the end of the second wheat experiment (June 1994). The initial ¹³C-depletion in SOC of cotton FACE plots (measured by the difference in ¹³C between FACE and Control plots) persisted at the same level (1.9) 1.5 years after the experiment ended. A similar depletion was observed in soil CO₂ evolved in the same plots, indicating ongoing decomposition of the new SOC. The SOC ¹³C of wheat plots before and after two growing seasons showed increasing ¹³C-depletion in FACE relative to Control. Isotopic mass balance was consistent with 5–6% new carbon input from the two wheat crops. This is lower than the 12–13% calculated for FACE cotton and perhaps a consequence of the larger root system of cotton or the 3-year duration of the cotton experiments versus 2 years for the wheat.