Ecological status of some Finnish rivers evaluated using benthic diatom communities

The applicability of some diatom methods to quality assessment of differenttypes of Finnish rivers was compared using dominant taxa of diatomcommunities and nutrient level estimations using Trophic Diatom Index(TDI) and a new Phosphorus Diatom Equation (PDE) [Tot. P_calc (g l^-1) = 39 – 0.278 ol-% – 0.117 olme-% – 0.346me-% – 0.006 meeu-% + 1.193 eu-%; n = 97; r² = 0.742;SE = 17.4]. The overall quality estimations were done by Index ofPollution Sensitivity (IPS) and by Generic Diatom Index (GDI); limit valuesfor quality classes are proposed.     

Uncertainty in ecological status assessments of lakes and rivers using diatoms

The EU’s Water Framework Directive requires all surface water bodies to be classified according to their ecological status. As biological communities show both spatial and temporal heterogeneity, expressions of ecological status will, inevitably, have an element of uncertainty associated with them. A consequence of this environmental heterogeneity is that there is a risk that status inferred from one or more samples is different to the true status of that water body. In order to quantify the scale of temporal uncertainty associated with benthic diatoms, replicate samples were collected from sites across the ecological status gradient in lakes and rivers in the UK. Variability (expressed as standard deviation of temporal replicate samples from a single site) could be described using a polynomial function and this was then used to calculate the risk of placing a water body in the wrong ecological status class. This risk varied depending on the distance from the class boundaries and the number of replicates. Based on these data, we recommend that ecological status is determined from a number of samples collected from a site over a period of time.     

AusRivAS: using macroinvertebrates to assess ecological condition of rivers in Western Australia

1. AusRivAS (Australian River Assessment Scheme) models were developed, using macroinvertebrates as indicators, to assess the ecological condition of rivers in Western Australia as part of an Australia-wide program. The models were based on data from 188 minimally disturbed reference sites and are similar to RIVPACS models used in Britain. The major habitats in the rivers (macrophyte, channel) were sampled separately and macroinvertebrates collected were identified to family level. 2. Laboratory sorting of preserved macroinvertebrate samples recovered about 90% of families present when 150 animals were collected, whereas live picking in the field recovered only 76%. 3. Reference sites clustered into five groups on the basis of macroinvertebrate families present. Using seven physical variables, a discriminant function allocated 73% of sites to the correct classification group. A discriminant function based on seven physical and two chemical variables allocated 81% of sites to the correct group. However, when the same reference sites were re-sampled the following year, the nine variable discriminant function misallocated more sites than the seven variable function, owing to annual fluctuations in water chemistry that were not accompanied by changes in fauna. 4. In preliminary testing, the wet season channel model correctly assessed 80% of reference sites as undisturbed in the year subsequent to model building (10% of sites were expected to rate as disturbed because the 10th percentile was used as the threshold for disturbance). Nine sites from an independent data set, all thought to be disturbed, were assessed as such by the model. Results from twenty test sites, chosen because they represented a wide range of ecological condition, were less clear-cut. In its current state the model reliably distinguishes undisturbed and severely disturbed sites. Subtle impacts are either detected inconsistently or do not affect ecological condition.     

Assessment of ecological status in U.K. rivers using diatoms

1. The European Union's Water Framework Directive requires all water bodies to achieve ‘good ecological status’ by 2015 and this paper describes a rationale for defining ‘good ecological status’ based on diatoms, a significant component of the biological quality element ‘macrophyte and phytobenthos’. 2. A database of benthic diatom samples collected over the past 20 years was assembled. New sampling, specifically for this project, was undertaken during 2004 to supplement these data. In total 1051 samples were included in the database with matching environmental data. 3. ‘Reference sites’, relatively unimpacted by human activity, were selected from this database by a series of screening steps and these sites were used to develop a site‐specific reference typology. 4. Environmental variables not related to the pressure gradient were used to predict the ‘expected’ Trophic Diatom Index (TDI) values at each site. Site‐specific TDI predictions were used to generate ecological quality ratios (EQRs) ranging from ≥1, where the diatom assemblage showed no impact, to (theoretically) 0, when the diatom assemblage was indicative of major anthropogenic activities. 5. The boundary between ‘high’ and ‘good’ status was defined as the 25th percentile of EQRs of all reference sites. The boundary between ‘good’ and ‘moderate’ status was set at the point at which nutrient‐sensitive and nutrient‐tolerant taxa were present in equal relative abundance. An ecological rationale for this threshold is outlined in the paper.     

Validation of diatoms as proxies for phytobenthos when assessing ecological status in lakes

Research to develop tools to assess the ecological status of phytobenthos, as required in Annex V of the European Union Water Framework Directive, has focussed largely on diatoms. Diatoms are often the most abundant and diverse group of algae within the phytobenthos and have been used widely for other monitoring purposes. However, there is little empirical justification for the use of diatoms as proxies for the wider phytobenthos. In this paper, we re-examine an existing dataset compiled largely from littoral samples from standing waters in the English Lake District and compared transfer functions for total phosphorus, dissolved inorganic carbon, conductivity and calcium concentration generated from diatoms and non-diatoms separately and together. The results show that transfer functions generated from diatoms alone are as powerful as transfer functions generated from diatoms and non-diatoms combined, while transfer functions generated from non-diatoms alone are less effective. These results provide support for the use of diatoms as proxies for phytobenthos when ecological status is being assessed. Handling editor: J. Saros     

Evaluating the ecological status of rivers using an index of ecological distance: An application to diatom communities

Community structure changes with pollution or stress. In the Water Framework Directive, high ecological status through biological parameters is defined as a slight or minor deviation from the reference community, while the good status is defined as a small deviation.To assess the importance of this deviation, and then to measure the degradation of ecological status along a river, an index based on the concept of “ecological distance” between species was created and called ecological distance index (EDI). It was tested on diatom data from a pilot watershed (the Garonne river basin, South-West France).The results show a good correlation between the EDI and the IPS (Indice de Pollusensibilité Spécifique – specific pollusensitivity index – a diatom-based biotic index) ecological ratios, which means first that the EDI is a valuable indicator of ecological status, and that it can account for ecoregional specificities. This index can be applied to any communities (macro-invertebrates, fish, etc.), since: (i) typical reference communities are found for each river type; (ii) species are characterised then ranked by pollution sensitivity values.     

Use of benthic diatom communities to evaluate water quality in rivers of southern Poland

Biological and chemical data were processed to estimate trophic stage and degree of pollution in several streams and rivers in southern Poland. The majority were eutrophic and some of them heavily polluted; only a few were oligo-mesotrophic. The differences in the water quality of the rivers were reflected by different types of diatom community and also by the values for some diatom indices, which were calculated using the latest version of the 'Omnidia' database software. Except for the Sládeček's index, all diatom indices correlated significantly with organic load (COD), oxygen concentration, conductivity and most of the measured ions. Some indices showed a significant negative correlation with trophic level (expressed by NH₄-N and PO₄-P). In general, IPS (Specific Pollution Sensitivity Index) and GDI (Generic Diatom Index) indices gave the best results. Among the investigated diatom communities, only a few taxa indicated oligo-mesotrophy and oligo-β-mesosaprobity. Most of the sites were characterised by a greater relative contribution of eutraphent and tolerant ones as well as α-mesosaprobic and polysaprobic diatoms. This study suggests that the structure of benthic diatom communities and diatom indices, especially GDI, can be applied for monitoring rivers in Poland. This revised version was published online in June 2006 with corrections to the Cover Date.     

Dynamics of diatom colonization process in some rivers influenced by urban pollution (Hanoi, Vietnam)

Periphytic diatom communities’ colonization patterns were studied at three sampling stations of the Red–Nhue–Tolich hydrosystem presenting different urban pollution levels by using artificial substrates for 6 weeks in dry season 2005. Structural characteristics of periphytic diatoms developed on glass substrates at each sampling site were followed and compared. This experiment showed, through various general criteria (total diatom density, dry weight biomass) and specific criteria (relative diatom abundances, indices) that the structure of benthic diatoms developed on these substrates was strongly affected by pollution as early as the second week of colonization. Communities exposed to heavily and moderately polluted sites contained species which are known to be saprophilous or tolerant to organic pollution such as Nitzschia umbonata, Nitzschia palea, Cyclotella meneghiniana, Eolimna minima. Growth inhibition of diatom communities at the heavily polluted site was mostly related to a strong increase of organic load rather than to low metallic input, though metallic (Cd and Zn) burdens have been successfully quantified in the biofilms developed at the three studied sites. Nevertheless, no significant difference in species richness and diversity index between colonization duration times was observed. Based on values of diatom indices Indice de Polluosensibilité (IPS) and diatom assemblage index to organic water pollution (DAIPo), water quality could be classified as medium quality at Red site, polluted at NT₂ and heavily polluted at TL. Thus, the use of diatoms as a tool for water assessment appears suitable for monitoring rivers in Vietnam, as it is in several other countries.     

Applicability of a generic index for diatom assemblages to monitor pollution in the tropical River Tsanwun, Taiwan

A generic index (GI) utilizing epilithic diatom assemblages as abioindicator of water pollution in subtropical rivers in Taiwan was applied toastudy of a tropical river. Seven sites on the River Tsanwun were sampledseasonally from August 1995 to June 1998. Although there is a marked differencein water chemistry between tropical and subtropical rivers in Taiwan, thedominant genera of their diatom assemblages are quite similar. The six generaused for the calculation of GI in the subtropical rivers were also the maincomponents of diatom assemblages in the River Tsanwun. The calculated valuesdisplayed a close correlation with water quality, evaluated on the basis ofphysical and chemical variables. A strong correlation was found between this GIand other diatom-based indices of water quality.     

Validation of the Macrophyte Quality Index (MaQI) set up to assess the ecological status of Italian marine transitional environments

The paper couples the results obtained by applying the expert and the rapid Macrophyte Quality Indices set up to assess the ecological status of the Italian transitional environments according to the requirements by the Water Framework Directive (2000/60/CE). The indices were validated by comparing the composition of the macrophyte assemblages and the values of some bio-physico-chemical parameters of the water column of 20 stations of the Venice lagoon sampled monthly for one year between 2003 and 2005. In 5 stations out of the 20, the ones which fall within the 5 classes of ecological status suggested by the Water Framework Directive, sedimentation rates, sediment grain-size, and nutrient and pollutant (metals, Polychloro-Dibenzo-Dioxins/Furans, Polycyclic Aromatic Hydrocarbons, Pesticides and Polychlorinated Biphenils) concentrations in surface sediments were also determined. Results showed strong relationships between the trends of these environmental parameters and the composition and structure of macrophyte associations, as well as with the Macrophyte Quality Index assessment. Chlorophyceae showed a trend opposite to Rhodophyceae whose presence was concentrated in oxygenated and transparent environments. Chlorophyceae and the species characterised by low scores prevailed in turbid areas where nutrient and pollutant concentrations were high. Results allowed the identification of the conditions of the “reference sites” (confinement areas and sites with high water renewal) and the integration of the dichotomic key used for the application of the R-MaQI. Handling editor: S. M. Thomaz     

The rivers Rhine and Meuse in The Netherlands: present state and signs of ecological recovery

The ecosystems of the rivers Rhine and Meuse have suffered drastic environmental changes, for example because of the regulation of the stream bed and the construction of weirs and dams. Furthermore, discharges of industrial and municipal waste water have caused the water quality of these rivers to deteriorate; this problem became acute in the sixties and seventies. Recently some chemical parameters of water quality have improved in the Rhine, and as a consequence some aquatic communities are showing signs of recovery after decades of severe degradation. This paper describes the present state of the aquatic communities in the Dutch part of the rivers Rhine and Meuse, using published observations on plankton, macrophytes, invertebrates, and fish. The sparse information on the food chain in these rivers is summarized. The main channels of the Rhine and Meuse are characterized by a dense plankton that develops rapidly in the nutrient-rich river waters. The stream beds, now dominated by wave-exposed sand and gravel, have a sparse fauna and flora. The river banks, mostly consolidated by blocks of stone, offer a substratum for numerous benthic organisms, particularly now that the water quality has improved. The floodplain waters and old river channels harbour a flora and fauna rich in species. The degree of water exchange with the river is crucial for the ecological development of the river and its backwaters. Today the freshwater tidal reaches of the rivers occupy a very restricted area, and only remnants of the previously abundant vegetation of rushes are found. Losses in the numbers of animal and plant species, notably those specific to rivers, are evident, but over the last 15 years several species have returned. Allochthonous species (exotics), including crustaceans and molluscs, have also settled in the Rhine and Meuse. Fish species characteristic of these rivers, such as river lamprey, sea trout, barbel, and flounder, have recently been observed in appreciable numbers. The Rhine Action Programme provides a framework for the large-scale rehabilitation of the Rhine. Experiments on re-stocking the Rhine with Atlantic salmon and on the ecological rehabilitation of floodplains are being carried out on the assumption that there will be a further reduction of the pollution load. A similar programme is required for the Meuse.     

How ecological indicators construction reveals social changes—The case of lakes and rivers in France

Social and scientific factors are deeply enmeshed in each other within the development and the use of ecological indicators (EI). Yet low research has assessed which factors contribute to selecting ecological indicators on the long-term. This article proposes to study the historical construction of EI by examining ecological, political, and social background of specific places where EI were developed, in France on lakes and rivers. Our major findings in France were that ecological indicators were never optimised for the present market or political arena. Instead EI development was typically recycling previous tools that were elaborated for other purposes by environmentally committed outsiders, without regular funding. We found that regular funding for monitoring an EI was only provided when it matched an institution's goal. Beyond the geographically limited relevance of the case studies, these results therefore improve the theoretical framework we deploy when constructing or relying on indicators.     

A method for prioritizing the reestablishment of river continuity in Austrian rivers

River continuity is one of the hydro-morphological elements supporting the classification of the ecological status of rivers. In order to achieve good ecological status in the continuity of rivers, the impacts of anthropogenic activities must be limited to the extent that some fish age classes may be missing and there may only be slight changes in species composition and abundance from the type specific community. The main goal of the project is to list priorities for removing obstruction to migration within 12 years to reestablish river continuity so as to allow undisturbed migration of aquatic organisms. The ecological status of the river, the cost–benefit ratio of the proposed restoration and the distance between obstructions are analysed. More than 200 obstructions in about 170 km of river were evaluated. A 1st ranking of the parameter “extension of undisturbed river length” shows significant alterations in comparison with the 2nd and 3rd ranking, where the river length is weighted over the parameters “ecological status” of the river reach and the “cost–benefit ratio” of the measure. The ecological status is classified by comparing the present versus the potential natural morphological conditions (Leitbild). The cost–benefit ratio takes into account the increase of the fish species composition and the cost of the measures in relation to local circumstances of available property and height of the drop. Examples of obstructions in the Pinka R. move back in the priority list to a maximum of −9 places and forwards to a maximum of +8 places. As a result the list of obstructions by priority for removal to be removed first at the top is analysed. The financial policy and a schedule for removal for the period 2003–2015 are based upon the results of the priority list. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Guest editors: R. L. Welcomme & G. Marmulla Hydropower, Flood Control and Water Abstraction: Implications for Fish and Fisheries     

A primer on the study of transitory dynamics in ecological series using the scale-dependent correlation analysis

Here we describe a practical, step-by-step primer to scale-dependent correlation (SDC) analysis. The analysis of transitory processes is an important but often neglected topic in ecological studies because only a few statistical techniques appear to detect temporary features accurately enough. We introduce here the SDC analysis, a statistical and graphical method to study transitory processes at any temporal or spatial scale. SDC analysis, thanks to the combination of conventional procedures and simple well-known statistical techniques, becomes an improved time-domain analogue of wavelet analysis. We use several simple synthetic series to describe the method, a more complex example, full of transitory features, to compare SDC and wavelet analysis, and finally we analyze some selected ecological series to illustrate the methodology. The SDC analysis of time series of copepod abundances in the North Sea indicates that ENSO primarily is the main climatic driver of short-term changes in population dynamics. SDC also uncovers some long-term, unexpected features in the population. Similarly, the SDC analysis of Nicholsons blowflies data locates where the proposed models fail and provides new insights about the mechanism that drives the apparent vanishing of the population cycle during the second half of the series.     

Generational differences in selenium status of women

In this cross-sectional study of three generations of women, daughters (19–26 yr), mothers (40–58 yr) and maternal grandmothers (67–84 yr) from the same 10 families in central Ohio were studied to determine the effect of life-cycle differences, including estrogen status, on selenium status. Plasma and red blood cell (RBC) selenium and glutathione peroxidase (GPx) activities were determined and typical dietary selenium intakes were calculated from food-frequency questionnaires. Selenium status was lowest in the oldest generation. Plasma selenium of daughters and grandmothers were significantly lower than those of mothers, and plasma GPx and RBC selenium of grandmothers were also lower than those of the mothers. A positive correlation (r=0.42, p<0.04) was found between plasma estrogen and plasma selenium concentrations. Selenium intakes of all groups were adequate and no differences in selenium intakes were found among groups. The results of this study indicate that selenium status fluctuates during the female life cycle and is related to estrogen status.     

An assessment of macrophyte community metrics in the determination of the ecological condition and total phosphorus concentration of Mediterranean ponds

The macrophyte vegetation of 51 ponds situated in the Duero river basin on the Northern Iberian Plateau was studied with the aim of selecting metrics that responded clearly to perturbation and that should be included in a multimetric index for assessing the ecological condition (expression of the quality of the structure and functioning of aquatic ecosystems associated with surface waters) of Mediterranean ponds. Furthermore, the specific response of metrics to the total phosphorus (TP) concentration was also investigated because of agricultural activities, which usually increase the concentration of nutrients in aquatic systems, are one of the principal types of impairment affecting ponds in the Duero basin.A total of 19 metrics representing several aspects of the structure of macrophyte communities (cover, richness, diversity) were selected as potential metrics.The strong overlap which was observed for macrophyte metrics between classes of ecological conditions (bad, poor, moderate, good, best available) and TP concentrations (>600, 600-300, 300-100, 100-50, <50 μg L⁻¹ TP) constitutes a major problem in defining and separating these classes. For this reason, Mann-Whitney U-test and discrimination efficiency were carried out to determine which of the measures best discriminated between slightly impaired sites (ponds in good or best available condition) and perturbed ponds (ponds in moderate, poor or bad condition). Most of the metrics showed significant differences (p < 0.05) between slightly impaired and perturbed ponds. However, only total cover and hydrophyte richness, which had the highest discrimination efficiency (>78%), showed no inter-quartile overlap (25th-75th percentile) between the two ecological classes. For these reasons, both total cover and hydrophyte richness were selected for the implementation of a multimetric index able to discriminate between slightly impaired and degraded Mediterranean flatland ponds.Currently, eutrophication is considered the main pressure on lakes. However, macrophyte metrics were far less sensitive to TP concentration than to ecological condition changes. This shows that other pressures are also of considerable importance. Thus, the definition of the ecological condition of lakes using macrophytes should not be based only on responses to eutrophication pressure, as has been done in some European countries.     

Applicability of ecological evaluation tools in estuarine ecosystems: the case of the lower Mondego estuary (Portugal)

In accordance with the Water Framework Directive guidelines (WFD, 2000, European Communities Official Journal L327 2000/60/EC), classification schemes and ecological evaluation tools (based on benthic invertebrate fauna data sets from 1990 to 2002) were applied in the lower Mondego estuary. Two distinct scenarios could be tested due to the implementation of mitigation practices in 1999, following a long eutrophication process, which started by the early 1980s. Some discrepancies in the results were found by the application of the different indices. The AMBI index (accounting for taxonomic composition) and the ABC method (accounting for abundance and biomass k-dominance patterns) classifications often disagreed with those based on species diversity (Margalef and Shannon-Wiener). The ambiguous results made the classification a complex task to achieve, contrary to the Directive’s objective of maintaining it simple and clear. Our results suggest the necessity of adjusting some of the indices and their ranges to estuarine characteristics, namely to account the typical dominance and abundance of some particular species. These aspects are not taken into consideration by some of the indices proposed, which are more adapted to typical marine conditions. Based on our results, these widely applied indices might still improve their efficiency in estuarine systems allowing their use in the resembling types already established within the new Directive agenda.