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Responsiveness of different citrus genotypes to the Xanthomonas citri ssp. citri‐derived pathogen‐associated molecular pattern (PAMP) flg22 correlates with resistance to citrus canker 下载免费PDF全文
Qingchun Shi Vicente J. Febres Jeffrey B. Jones Gloria A. Moore 《Molecular Plant Pathology》2015,16(5):507-520
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Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy‐associated vacuolar processes 下载免费PDF全文
Roxana A. Roeschlin María A. Favaro María A. Chiesa Sergio Alemano Adrián A. Vojnov Atilio P. Castagnaro María P. Filippone Frederick G. Gmitter jr José Gadea María R. Marano 《Molecular Plant Pathology》2017,18(9):1267-1281
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An engineered promoter driving expression of a microbial avirulence gene confers recognition of TAL effectors and reduces growth of diverse Xanthomonas strains in citrus 下载免费PDF全文
Deepak Shantharaj Patrick Römer Jose F. L. Figueiredo Gerald V. Minsavage Christina Krönauer Robert E. Stall Gloria A. Moore Latanya C. Fisher Yang Hu Diana M. Horvath Thomas Lahaye Jeffrey B. Jones 《Molecular Plant Pathology》2017,18(7):976-989
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The YaeQ family of proteins are found in many Gram-negative and a few Gram-positive bacteria. We have determined the first structure of a member of the YaeQ family by X-ray crystallography. Comparisons with other structures indicate that YaeQ represents a new compact protein fold built around a variation of the PD-(D/E)XK nuclease motif found in type II endonucleases and enzymes involved in DNA replication, repair, and recombination. We show that catalytically important residues in the PD-(D/E)XK nuclease superfamily are spatially conserved in YaeQ and other highly conserved YaeQ residues may be poised to interact with nucleic acid structures. 相似文献
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Michele Leonardi Katarzyna E. Ambryszewska Bernardo Melai Guido Flamini Pier Luigi Cioni Federico Parri Luisa Pistelli 《化学与生物多样性》2013,10(3):343-355
The composition of 21 essential‐oil samples isolated from Helichrysum italicum collected in seven locations of Elba Island (Tuscany, Italy), characterized by different soil types, during three different periods (January, May, and October 2010) was determined by GC‐FID and GC/EI‐MS analyses. In total, 115 components were identified, representing 96.8–99.8% of the oil composition. The oils were characterized by a high content of oxygenated monoterpenes (38.6–62.7%), while monoterpene and sesquiterpene hydrocarbons accounted for 2.3–41.9 and 5.1–20.1% of the identified constituents, respectively. The main oxygenated derivatives were nerol (2.8–12.8%) and its ester derivative neryl acetate (5.6–45.9%). To compare the chemical variability of the species within Elba Island and between the island and other localities within the Mediterranean area, studied previously, multivariate statistical analysis was performed. The results obtained showed a difference in the composition of the essential oils of H. italicum from Elba Island, mainly due to the environment where the plant grows, and, in particular, to the soil type. These hypotheses were further confirmed by the comparison of these oils with essential oils obtained from H. italicum collected on other islands of the Tuscan archipelago. 相似文献
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Genetic mapping and localization of a major QTL for seedling resistance to downy mildew in Chinese cabbage (Brassica rapa ssp. pekinensis) 总被引:1,自引:0,他引:1
Shuancang Yu Fenglan Zhang Renbo Yu Yanmin Zou Jiani Qi Xiuyun Zhao Yangjun Yu Deshuang Zhang Li Li 《Molecular breeding : new strategies in plant improvement》2009,23(4):573-590
Downy mildew caused by the fungus Peronospora parisitica is a serious threat to members of the Brassicaceae family. Annually, a substantial loss of yield is caused by the widespread
presence of this disease in warm and humid climates. The aim of this study was to localize the genetic factors affecting downy
mildew resistance in Chinese cabbage (Brassica rapa ssp. pekinensis). To achieve this goal, we improved a preexisting genetic map of a doubled-haploid population derived from a cross between
two diverse Chinese cabbage lines, 91-112 and T12-19, via microspore culture. Microsatellite simple sequence repeat (SSR)
markers, isozyme markers, sequence-related amplified polymorphism markers, sequence-characterized amplified region markers
and sequence-tagged-site markers were integrated into the previously published map to construct a composite Chinese cabbage
map. In this way, the identities of linkage groups corresponding to the Brassica A genome reference map were established. The new map contains 519 markers and covers a total length of 1,070 cM, with an
average distance between markers of 2.06 cM. All markers were designated as A1–A10 through alignment and orientation using
55 markers anchored to previously published B. rapa or B. napus reference maps. Of the 89 SSR markers mapped, 15 were newly developed from express sequence tags in Genbank. The phenotypic
assay indicated that a single major gene controls seedling resistance to downy mildew, and that a major QTL was detected on
linkage group A8 by both interval and MQM mapping methods. The RAPD marker K14-1030 and isozyme marker PGM flanked this major
QTL in a region spanning 2.9 cM, and the SSR marker Ol12G04 was linked to this QTL by a distance of 4.36 cM. This study identified
a potential chromosomal segment and tightly linked markers for use in marker-assisted selection to improve downy mildew resistance
in Chinese cabbage. 相似文献
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Dimitra Kovala-Demertzi Mavroudis A. Demertzis Eleni Filiou Anastasia A. Pantazaki Paras N. Yadav John R. Miller Yifan Zheng Dimitrios A. Kyriakidis 《Biometals》2003,16(3):411-418
The reactions of Pd(II) and Pt(II) with 2-Acetyl Pyridine N(4)-Ethyl-Thiosemicarbazones, HAc4Et and 2-Acetyl Pyridine N(4)-1-(2-pyridyl)-piperazinyl Thiosemicarbazone, HAc4PiPiz and 2-Formyl Pyridine N(4)-1-(2-pyridyl)-piperazinyl Thiosemicarbazone, HFo4PiPiz afforded the complexes, [Pd(Ac4Et)], 1, [Pd(HAc4Et)2]Cl2, 2 and [Pd(Ac4Et)2], 3[Pt(Ac4Et)], 4, [Pt(HAc4Et)2]Cl2, 5, [Pt(Ac4Et)2], 6 and [Pd(Fo4PipePiz)Cl], 7, [Pd(Fo4PipePiz)2], 8, [Pd(Ac4PipePiz)Cl], 9 and [Pd(Ac4PipePiz)2], 10. The crystal structure of the complex [Pt(Ac4Et)2], 6 has been solved. The platinum(II) atom is in a square planar environment surrounded by two cis nitrogen atoms and two cis sulfur atoms. The ligands are not equivalent, one being tridentate with (N,N,S) donation, the other being monodentate using only the sulfur atom to coordinate to the metal. The tridentate ligand shows a Z, E, Z configuration while the monodentate ligand shows an E, E, Z. Inter-molecular hydrogen bonds stabilize the structure, while the crystal packing is determined by –, and Pt – C interactions. The antibacterial effect of Pd(II) and Pt(II) complexes were studied in vitro. The complexes were found to have effect on Gram(+) bacteria, while the same complexes showed no bactericidal effect on Gram(–) bacteria. The effect of the Pd(II) and Pt(II) complexes on the in vitro DNA strand breakage was studied by agarose gel electrophoresis. The complexes 1-6 were found to exhibit a cytotoxic potency in a very low micromolar range and to be able to overcome the cisplatin resistance of A2780/Cp8 cells (Kovala-Demertzi et al. 2000). 相似文献