共查询到3条相似文献,搜索用时 15 毫秒
1.
Bahram Zargar Syed A. Sattar Richard Kibbee Joseph Rubino M. Khalid Ijaz 《Journal of applied microbiology》2022,132(2):1489-1495
2.
T.L. Buhr A.A. Young Z.A. Minter C.M. Wells D.C. McPherson C.L. Hooban C.A. Johnson E.J. Prokop J.R. Crigler 《Journal of applied microbiology》2012,113(5):1037-1051
Aims
To develop test methods and evaluate the survival of Bacillus anthracis ?Sterne and Bacillus thuringiensis Al Hakam spores after exposure to hot, humid air.Methods and Results
Spores (>7 logs) of both strains were dried on six different test materials. Response surface methodology was employed to identify the limits of spore survival at optimal test combinations of temperature (60, 68, 77°C), relative humidity (60, 75, 90%) and time (1, 4, 7 days). No spores survived the harshest test run (77°C, 90% r.h., 7 days), while > 6·5 logs of spores survived the mildest test run (60°C, 60% r.h., 1 day). Spores of both strains inoculated on nylon webbing and polypropylene had greater survival rates at 68°C, 75% r.h., 4 days than spores on other materials. Electron microscopy showed no obvious physical damage to spores using hot, humid air, which contrasted with pH‐adjusted bleach decontamination.Conclusions
Test methods were developed to show that hot, humid air effectively inactivates B. anthracis ?Sterne and B. thuringiensis Al Hakam spores with similar kinetics.Significance and Impact of the Study
Hot, humid air is a potential alternative to conventional chemical decontamination. 相似文献3.
Exposure to Aspergillus fumigatus is linked with respiratory diseases such as asthma, invasive aspergillosis, hypersensitivity pneumonitis, and allergic bronchopulmonary
aspergillosis. Molecular methods using quantitative PCR (qPCR) offer advantages over culture and optical methods for estimating
human exposures to microbiological agents such as fungi. We describe an assay that uses lyticase to digest A. fumigatus conidia followed by TaqMan™ qPCR to quantify released DNA. This method will allow analysis of airborne A. fumigatus samples collected over extended time periods and provide a more representative assessment of chronic exposure. The method
was optimized for environmental samples and incorporates: single tube sample preparation to reduce sample loss, maintain simplicity,
and avoid contamination; hot start amplification to reduce non-specific primer/probe annealing; and uracil-N-glycosylase to prevent carryover contamination. An A. fumigatus internal standard was developed and used to detect PCR inhibitors potentially found in air samples. The assay detected fewer
than 10 A. fumigatus conidia per qPCR reaction and quantified conidia over a 4−log10 range with high linearity (R
2 > 0.99) and low variability among replicate standards (CV=2.0%) in less than 4 h. The sensitivity and linearity of qPCR for
conidia deposited on filters was equivalent to conidia calibration standards. A. fumigatus DNA from 8 isolates was consistently quantified using this method, while non-specific DNA from 14 common environmental fungi,
including 6 other Aspergillus species, was not detected. This method provides a means of analyzing long term air samples collected on filters which may
enable investigators to correlate airborne environmental A. fumigatus conidia concentrations with adverse health effects. 相似文献