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1.
Summary A technique has been developed for the investigation of the photopigment involved in the photoperiodic control of reproduction in Japanese quail, Coturnix coturnix. When these photoreceptors were exposed to white or monochromatic light a clear relationship was found between light intensity and the extent of photo-induced luteinizing hormone (LH) secretion. A spectroradiometric investigation of the passage of light through the skull and brain enabled us to illuminate the hypothalamic region with equal numbers of photons at a range of wavelengths. Action spectra were then conducted and showed a photopigment with a peak sensitivity at wavelengths near 500 nm. An excellent match was obtained when the standard absorption spectrum for a rhodopsin was fitted to the action spectrum, suggesting a rhodopsin maximally sensitive at 492 nm. The absolute sensitivity of the photoreceptors was calculated at a range of wavelengths: with light at 500 nm, 2.85×10 –12 E·cm –2·s –1 triggered the photoperiodic response. This level of sensitivity is matched only by the rhodopsin visual pigments.Abbreviations
LH
luteinizing hormone
-
T
transmission 相似文献
2.
The spectral absorption characteristics of the retinal photoreceptors of the blue tit ( Parus caeruleus) and blackbird ( Turdus merula) were investigated using microspectrophotometry. The retinae of both species contained rods, double cones and four spectrally
distinct types of single cone. Whilst the visual pigments and cone oil droplets in the other receptor types are very similar
in both species, the wavelength of maximum sensitivity (λ max) of long-wavelength-sensitive single and double cone visual pigment occurs at a shorter wavelength (557 nm) in the blackbird
than in the blue tit (563 nm). Oil droplets located in the long-wavelength-sensitivesingle cones of both species cut off wavelengths
below 570–573 nm, theoretically shifting cone peak spectral sensitivity some 40 nm towards the long-wavelength end of the
spectrum. This raises the possibility that the precise λ max of the long-wavelength-sensitive visual pigment is optimised for the visual function of the double cones. The distribution
of cone photoreceptors across the retina, determined using conventional light and fluorescence microscopy, also varies between
the two species and may reflect differences in their visual ecology.
Accepted: 8 January 2000 相似文献
3.
Summary This study reports photopic spectral sensitivity curves (351–709 nm) for four individual roach, Rutilus rutilus, determined by two choice appetitive training. All four curves show four sensitivity maxima at 361–398 nm, 421–448 nm, 501–544 nm and 634–666 nm which are related to the four known roach photopic visual pigments (Avery et al. 1982). The overall shape of the curves at long wavelengths indicates inhibitory interactions between the red and green cone mechanisms. That the high behavioural sensitivity in the UV is caused by a specific ultraviolet visual pigment and is not due to aberrant stimulation of the other cone types is shown by the redetermination of spectral sensitivity at short wavelengths (351–501 nm) following the selective bleaching of the three longer wavelength visual pigments. This depresses the blue sensitivity to a greater degree than the relatively unaffected UV sensitivity maximum. Spectral transmission data from two corneas and four lenses show that they transmit considerable amounts of light in the near UV. 相似文献
4.
Ceratium fusus (Ehrenb.) Dujardin was exposed to light of different wavelengths and photon flux densities (PFDs) to examine their effects on mechanically stimulable bioluminescence (MSL). Photoinhibition of MSL was proportional to the logarithm of PFD. Exposure to I μmol photons·m ?2s ?1 of broadband blue light (ca. 400–500 nm) produced near-complete photoinhibition (≥90% reduction in MSL) with a threshold at ca. 0.01 μmol photons·m ?2·s ?1. The threshold of photoinhibition was ca. an order of magnitude greater for both broadband green (ca. 500–580 nm) and red light (ca. 660–700 nm). Exposure to narrow spectral bands (ca. 10 nm half bandwidth) from 400 and 700 nm at a PFD of 0.1 μmol photons·m ?2·s ?1 produced a maximal response of photoinhibition in the blue wavelengths (peak ca. 490 nm). A photoinhibition response (≥ 10%) in the green (ca. 500–540 nm) and red wavelengths (ca. 680 nm) occurred only at higher PFDs (1 and 10 μmol photons·m ?2·s ?1). The spectral response is similar to that reported for Gonyaulax polyedra Stein and Pyrocystis lunula Schütt and unlike that of Alexandrium tamarense (Lebour) Balech et Tangen. The dinoflagellate's own bioluminescence is two orders of magnitude too low to result in self-photoinhibition. The quantitative relationships developed in the laboratory predict photoinhibition of bioluminescence in populations of C. fusus in the North Atlantic Ocean. 相似文献
5.
With a few clear exceptions (e.g., Daphnia) it is uncertain if most aquatic invertebrates can detect and respond to ultraviolet radiation (UVR). It is known that many aquatic invertebrates are vulnerable to UVR and that anthropogenically-induced increases in surface UVR have occurred in recent decades. We examined the photoresponses of late larval instars of Chaoborus punctipennis to different combinations of UVA (320–400 nm), UVB (300–320 nm) and visible light (400–700 nm) to determine whether the larvae can detect and/or avoid UVR. To accomplish this, we exposed late instar C. punctipennis larvae to a directional light source of UVR only (peak wavelength at 360 nm), visible light only or visible plus various wavebands of UVR. We examined negative phototaxis for 10 min at a quantum flux of 2.62 x 10 13 quanta s –1 cm –2 (S.D. = 3.63 x 10 12 quanta s –1 cm –2). In the dark, larvae stayed close to the surface of the experimental vessels. Under all treatments containing visible light the larvae exhibited negative phototaxis and occupied the bottom of the vessels. Under UVR only, the larvae occupied the middle of the water column. Our results suggest that late instar C. punctipennis larvae are unable to detect and avoid UVB and short UVA wavelengths but they can detect long UVA wavelengths. 相似文献
6.
The visual pigments and oil droplets in the retina of the diurnal gecko Gonatodes albogularis were examined microspectrophotometrically, and the spectral sensitivity under various adapting conditions was recorded using electrophysiological responses. Three classes of visual pigments were identified, with max at about 542, 475, and 362 nm. Spectral sensitivity functions revealed a broad range of sensitivity, with a peak at approximately 530–540 nm. The cornea and oil droplets were found to be transparent across a range from 350–700 nm, but the lens absorbed short wavelength light below 450 nm. Despite the filtering effect of the lens, a secondary peak in spectral sensitivity to ultraviolet wavelengths was found. These results suggest that G. albogularis does possess the visual mechanisms for discrimination of the color pattern of conspecifics based on either hue or brightness. These findings are discussed in terms of the variation in coloration and social behavior of Gonatodes.Abbreviations
ERG
electroretinogram
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MSP
microspectrophotometry
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UV
ultraviolet
- max
wavelength of maximum absorbance 相似文献
7.
The wavelength discrimination threshold of three goldfish was examined in a series of behavioral experiments. Using an auto-shaping technique, detection thresholds were established for 531 and 648 nm spectral increments presented on a 6.6 cd m –2 white background. Next, discrimination between the wavelengths was established at equal, suprathreshold, intensities. Finally, the intensities of the two stimuli were reduced to establish the intensity threshold for the wavelength discrimination. The results indicate that goldfish, like several mammalian species, can discriminate wavelength at detection threshold intensity. This finding suggests that high color sensitivity is not confined to mammals or dependent upon a very high percentage of wavelength opponent ganglion cells. Rather, high color vision sensitivity may be based upon an inherent sensitivity advantage of wavelength opponent receptive fields compared to non-wavelength opponent receptive fields and be an important selective advantage of wavelength opponency and color vision. 相似文献
8.
Electroretinographic recordings were made from hatchling loggerhead and leatherback sea turtle eyecup preparations to generate dark-adapted spectral sensitivity curves. Both species were maximally sensitive to wavelengths between 500 and 540 nm, with a secondary peak near 380 nm. The spectral sensitivity curve for leatherbacks was attenuated at the long wavelength end of the spectrum relative to that of the loggerheads. This difference may reflect adaptations to lighting available at the relatively shallow (loggerhead) versus deeper (leatherback) sites where each species forages. The broad spectrum of wavelengths detected by both species (near UV to yellow–orange) indicates that vision is likely mediated by more than one photopigment, potentially rendering these turtles capable of color vision. 相似文献
9.
Summary Spectral sensitivity of the cichlid fish Haplochromis burtoni was measured under both scotopic and photopic conditions using a two-choice, food reward, operant conditioning paradigm. The highest absolute sensitivity (scotopic) is one quantum for every 5 to 50 rods measured at 475 nm (equivalent to a corneal irradiance of 3.8×10 6 Q s –1 cm –2). A P500 1 photopigment apparently mediates spectral sensitivity over most of the visible spectrum; microspectrophotometric studies of rods had previously shown them to contain this photopigment. However, the scotopic behavioral action spectrum shows a sensitivity to short wavelength light higher than is consistent with a P500 1 photopigment alone mediating the scotopic visual process. Determinations made under photopic conditions reveal a behavioral action spectrum broader than that found under scotopic conditions and consistent with mediation by interaction of the three known cone types in an opponent processing manner. The calculated photopic threshold value of approximately 10 4 Q s –1 (receptor) –1 is in agreement with results from other species and corresponds to a corneal irradiance of about 7×10 10Q s –1cm –2. 相似文献
10.
The relative reflectance spectra for control and treated (surface wiped) current-year foliage of Douglas fir, and Sitka, Colorado, and Blue spruce ( Pseudotsuga menziesii [Mirb.] Franco, Picea sitchensis [Bong.] Carr., Picea pungens Engelm., and Picea pungens Engelm. var. hoopsii, respectively) were obtained from 220 to 700 nm. The green color of the control foliage of both Douglas fir and Sitka spruce was unaffected by the treatment whereas the blue-green and blue-white foliage of control Colorado and Blue spruce, respectively, became “green” as a result of the wiping. The relative reflectance curves for all green foliage, including the treated Colorado and Blue spruce, were all very similar with a peak in the green (540-560 nm), minima in the red (660-680 nm) and blue (450-500 nm), and very low reflectivities in the ultraviolet (λ < 400 nm). In contrast, the control foliage for Colorado and Blue spruce both showed a generally higher relative reflectance over most of the visible spectrum (400-700 nm) with a marked increase in the blue region (400-500 nm). At wavelengths below 420 nm, their relative reflectances increased sharply with decreasing wavelength, the reflectance at 220 nm for Blue spruce being over four times that at 540 nm. 相似文献
11.
Avian visual sensitivity encompasses both the human visible range (400–700 nm) and also near‐ultraviolet (UV) wavelengths (320–400 nm) invisible to normal humans. I used reflectance spectrophotometry to assess variation in UV reflectance for yellow, orange and red plumage in 67 species of tanager (Passeriformes). Previous chemical studies, and my analysis of reflectance minima, suggest that carotenoids are the dominant pigments in yellow, orange and red tanager plumage. Spectra recorded over the range of wavelengths to which birds are sensitive (320–700 nm) were invariably bimodal, with both a plateau of high reflectance at longer (> 500 nm) wavelengths and a distinct secondary peak at UV (< 400 nm) wavelengths. Within this overall framework, variation in UV reflectance was expressed within well‐defined quantitative limits: (1) peak reflectance was always lower than the corresponding plateau of reflectance at longer visible wavelengths; (2) the intensity of peak reflectance declined steadily below 350 nm; (3) wavelengths of peak reflectance clustered between 350 and 370 nm. Significant correlations were detected between various measures of total reflectance in the UV and visible wavebands, but not between various measures of spectral location of UV and visible reflectance. I propose that the strong absorption band at short visible wavelengths (~ 380–550 nm) responsible for bimodal spectra of carotenoids in vitro is also responsible for bimodal reflectance by carotenoid‐based plumage colours. The construction of the UV and visible reflectance bands from different sides of this same absorbance band provides a mechanism for the observed covariation between UV and visible wavelengths. Lack of an association between the spectral locations of the UV and visible reflectance bands may result from the limited variation in spectral location of the UV band. These patterns suggest that plumage colours are subject to constraints, just as are more traditional morphological characters. © 2005 The Linnean Society of London, Biological Journal of the Linnean Society, 2005, 84 , 243–257. 相似文献
12.
Caliothrips phaseoli, a phytophagous insect, detects and responds to solar ultraviolet-B radiation (UV-B; λ ≤ 315 nm) under field conditions. A highly specific mechanism must be present in the thrips visual system in order to detect this narrow band of solar radiation, which is at least 30 times less abundant than the UV-A (315–400 nm), to which many insects are sensitive. We constructed an action spectrum of thrips responses to light by studying their behavioural reactions to monochromatic irradiation under confinement conditions. Thrips were maximally sensitive to wavelengths between 290 and 330 nm; human-visible wavelengths ( λ ≥ 400 nm) failed to elicit any response. All but six ommatidia of the thrips compound eye were highly fluorescent when exposed to UV-A of wavelengths longer than 330 nm. We hypothesized that the fluorescent compound acts as an internal filter, preventing radiation with λ > 330 nm from reaching the photoreceptor cells. Calculations based on the putative filter transmittance and a visual pigment template of λmax = 360 nm produced a sensitivity spectrum that was strikingly similar to the action spectrum of UV-induced behavioural response. These results suggest that specific UV-B vision in thrips is achieved by a standard UV-A photoreceptor and a sharp cut-off internal filter that blocks longer UV wavelengths in the majority of the ommatidia. 相似文献
13.
The reversibly photochromic pigment, phycochrome c, was extracted from the blue-green alga Nostoc muscorum strain A. Action spectra were determined for in vitro conversions of the pigment from the short wavelength to the long wavelength form and vice versa. The action peak for the absorbance decrease at 650 nm is at 630 nm. During this decrease there is only a slight increase of the absorbance in the green region. Green and yellow light (maximum efficiency at 580 nm) completely restores absorbance at 650 nm. The observations are explained by the existence of three spectrally different forms of phycochrome c: P c630 and P c650 which equilibrate in darkness and P c580 which is reversibly photoconvertible to P c630. We have also measured the absorbance changes brought about by saturating irradiations with light of various wavelengths (“photostationary state spectrum”). Extreme photostationary states were obtained with about 650 nm and 500 nm light. 相似文献
14.
Summary In growing as well as in resting cells of a chlorophyll-free yellow mutant of Chlorella vulgaris (211-11h/20) synthesis of carotenoids is enhanced by blue light. Permanent irradiation is necessary to maintain the effect (Fig. 2). At wavelengths around 454 nm the additional carotenoid production is half-saturated at about 1000 erg cm -2s -1 and saturated at about 4000 erg cm -2s -1 (Fig. 3). An action spectrum exhibits highest efficiency of wavelengths around 465 and 370 nm, a minimum near 400 nm and inefficacy of yellow, red and far-red light (Fig. 4). This wavelength dependence resembles those of light enhanced O 2-uptake, carbohydrate consumption and gain of organically bound nitrogen by the organism. 相似文献
15.
Complexes of pig kidney diamine oxidase with azide, thiocyanate, and cyanide have been characterized by EPR and circular dichroism spectroscopy. Cu(II) d-d bands are observed in the CD spectrum of the resting enzyme at ≈800 nm (12 500 cm −1) and 575 nm (17 400 cm −1). Anion binding produces characteristic changes in the CD spectra. N 3−/SCN − → Cu(II) ligand-to-metal charge-transfer transitions are located at 390 nm (25 600 cm −1) and 365 nm (27 400 cm −1), respectively. In addition, an intense new band grew in at ≈500 nm (20 000 cm −1) when azide or thiocyanate were added, which may be assigned as a Cu(II) electronic transition that gains rotational strength in the anion complex. EPR spectra established that the Cu(II)-anion complexes are tetragonal; however, the magnitudes of the anion-induced shifts in the EPR parameters were consistent with substantial perturbations of the Cu(II) electronic ground state in the thiocyanate and cyanide complexes. Prominent superhyperfine splitting was apparent in the EPR spectra of the diamine oxidase complexes with thiocyanate and cyanide. The superhyperfine structure is (at least) partially attributable to endogenous Cu(II) ligands, probably from imidazole. 相似文献
16.
Photoluminescence, and optically stimulated luminescence in ZnB 2X 4 (B; Li,Na,K: X; Cl,Br) compounds doped with Cu + or Ag + were studied. Double humped emission bands attributable to the activators were observed in all the samples. The observed photoluminescence of Cu + and Ag + could be identified with 3d 94s 1?3d 10 and 4d 95s 1?5d 10 transitions respectively. The longer wavelength band (400–500 nm range) could be attributed to the Cu + or Ag + ion replacing alkali ion at the octahedral alkali site whereas short wavelength band (340–400 nm range) is attributed to a Cu or Ag ion at tetrahedral zinc site. The short wavelength band was found to be intense compared with long wavelength and gave an indication that most of the Cu or Ag ions prefered a tetrahedral Zn site compared with the octahedral alkali site. All the samples exhibit optically stimulated luminescence (OSL). The sensitivity was found to be lattice dependent. The lowest sensitivity of about 1% compared with Al 2O 3:C was observed in lithium lattices whereas highest the sensitivity of about 290% was observed in the case of Cu‐doped ZnNa 2Br 4. 相似文献
17.
The coherent 11- cis-retinal photoisomerization dynamics in bovine rhodopsin was studied by femtosecond time-resolved laser absorption spectroscopy
at 30-fs resolution. Femtosecond pulses of 500, 535, and 560 nm wavelength were used for rhodopsin excitation to produce different
initial Franck-Condon states and relevant distinct values of the vibrational energy of the molecule in its electron excited
state. Time evolution of the photoinduced rhodopsin absorption spectra was monitored after femtosecond excitation in the spectral
range of 400–720 nm. Oscillations of the time-resolved absorption signals of rhodopsin photoproducts represented by photorhodopsin 570 with vibrationally-excited all- trans-retinal and rhodopsin 498 in its initial state with vibrationally-excited 11- cis-retinal were studied. These oscillations reflect the dynamics of coherent vibrational wave-packets in the ground state of
photoproducts. Fourier analysis of these oscillatory components has revealed frequencies, amplitudes, and initial phases of
different vibrational modes, along which the motion of wave-packets of both photoproducts occurs. The main vibrational modes
established are 62, 160 cm −1 and 44, 142 cm −1 for photorhodopsin 570 and for rhodopsin 498, respectively. These vibrational modes are directly involved in the coherent reaction under the study, and their amplitudes
in the power spectrum obtained through the Fourier transform of the kinetic curves depend on the excitation wavelength of
rhodopsin. 相似文献
18.
In this paper, the simultaneous switching and sensing capabilities of a compact plasmonic structure based on a conventional rectangular hole in a silver film are proposed and investigated. The proposed structure has ultrahigh sensitivity up to 3000 nm/RIU and high figure of merit of 170 RIU−1. Also, the simulation results show the potential of the presented refractive index sensor to detect malaria infection, cancer cells, bacillus bacteria, and solution of glucose in water. Simultaneously, by changing the incident lightwave polarization, the structure behaves like a plasmonic switch, which has high extinction ratios of 15.81, 31.20, and 25.03 dB at three telecommunication wavelengths of 850, 1310, and 1550 nm, respectively. The ultrafast response time of 20 fs is achieved for the wideband application of the switching capability at the wavelength range of 1056 to 1765 nm. Moreover, the equivalent circuit model and transmission (ABCD) matrix methods are derived to validate the simulated results. Simple design, good agreement between the numerical and analytical results, biomedical applications, ultrahigh sensitivity, and ultrafast performance of the proposed structure help this idea to open up paths for design and implementation of other multi-application plasmonic devices in near-infrared region. To the best of our knowledge, the mentioned analytical methods have not been studied former at near-infrared wavelengths. Therefore, the achievements could pave the way for verifying the simulation results of plasmonic nanostructures in future investigations. 相似文献
19.
The light regime is an ecologically important factor in pelagic habitats, influencing a range of biological processes. However, the availability and importance of light to these processes in high Arctic zooplankton communities during periods of ''complete'' darkness (polar night) are poorly studied. Here we characterized the ambient light regime throughout the diel cycle during the high Arctic polar night, and ask whether visual systems of Arctic zooplankton can detect the low levels of irradiance available at this time. To this end, light measurements with a purpose-built irradiance sensor and coupled all-sky digital photographs were used to characterize diel skylight irradiance patterns over 24 hours at 79°N in January 2014 and 2015. Subsequent skylight spectral irradiance and in-water optical property measurements were used to model the underwater light field as a function of depth, which was then weighted by the electrophysiologically determined visual spectral sensitivity of a dominant high Arctic zooplankter, Thysanoessa inermis. Irradiance in air ranged between 1–1.5 x 10 -5
μmol photons m -2 s -1 (400–700 nm) in clear weather conditions at noon and with the moon below the horizon, hence values reflect only solar illumination. Radiative transfer modelling generated underwater light fields with peak transmission at blue-green wavelengths, with a 465 nm transmission maximum in shallow water shifting to 485 nm with depth. To the eye of a zooplankter, light from the surface to 75 m exhibits a maximum at 485 nm, with longer wavelengths (>600 nm) being of little visual significance. Our data are the first quantitative characterisation, including absolute intensities, spectral composition and photoperiod of biologically relevant solar ambient light in the high Arctic during the polar night, and indicate that some species of Arctic zooplankton are able to detect and utilize ambient light down to 20–30m depth during the Arctic polar night. 相似文献
20.
Summary The crustacean Daphnia magna responds to a flash of light with a ventral rotation of its compound eye; this behavior is termed eye flick. We determined the spectral sensitivity for the threshold of eye flick in response to light flashes having three different spatial characteristics: (1) full-field, extending 180° from dorsal to ventral in the animal's field of view; (2) dorsal, 30° wide and located in the dorsal region of the visual field; (3) ventral, same as dorsal but located ventrally. All three stimuli extended 30° to the right and to the left of the animal's midplane. We found that spectral sensitivity varies with the spatial characteristics of the stimulus. For full-field illumination, the relative sensitivity was maximal at 527 nm and between 365 nm and 400 nm, with a significant local minimum at 420 nm. For the dorsal stimulus, the relative sensitivity was greatest at 400 nm, but also showed local maxima at 440 nm and 517 nm. For the ventral stimulus, the relative sensitivity maxima occurred at the same wavelengths as those for the full-field stimulus. At wavelengths of 570 nm and longer, the responses to both dorsal and ventral stimuli showed lower relative sensitivity than the full-field stimulus. No circadian or other periodic changes in threshold spectral sensitivity were observed under our experimental conditions. Animals which had their nauplius eyes removed by means of laser microsurgery had the same spectral sensitivity to full-field illumination as normal animals. Our results are discussed in terms of our current knowledge of the spectral classes of photoreceptors found in the Daphnia compound eye. 相似文献
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