Interference between the macroalga Caulerpa prolifera and the seagrass Halodule wrightii

A field experiment was conducted to examine interactions between the seagrass Halodule wrightii and the macroalga Caulerpa prolifera in the Indian River Lagoon, FL, USA, and further if the outcome of the interactions between the two species was influenced by water depth. The experiment involved the manipulation of neighbor presence in plots established at the shallowest (50 cm) and deepest (80 cm) depths at which the two species co-existed at adequate densities to perform removal experiments. Shoot and frond densities were measured at the beginning (April), middle (July) and end (October) of the 6-month growing season, and above- and below-ground biomass values were determined at the end of the experiment (October). In the middle of the growing season H. wrightii had higher shoot densities and greater biomass in plots where C. prolifera had been removed at both water depths. This same pattern in shoot density and biomass for H. wrightii also occurred at the end of the growing season at the 80 cm depth. C. prolifera occurred at higher densities and greater biomass in the 80 cm depth range, but was generally unaffected by the presence of H. wrightii at either depth.     

Factors Affecting Restoration of Halodule wrightii to Galveston Bay, Texas

Experimental restoration of Halodule wrightii (shoalgrass) to its former range on Galveston Island, Texas, began in April 1994. We tested the effects of site, planting density, water depth, and fertilizer addition on survival and growth through June 1996. Temperature, salinity, light transmittance, turbidity, and sediment properties at two restoration sites, Redfish Cove and Snake Island Cove, were similar to those in naturally occurring grassbeds in nearby Christmas Bay. Halodule survival, coverage, and new shoot densities were affected by site (significantly higher at Redfish Cove than at Snake Island Cove, which eventually failed), by planting density (significantly higher when planted on 0.25-m or 0.5-m centers rather than on 1.0-m centers), and by water depth (significantly higher when planted in relatively shallow water). Propagation (spreading from transplant units) was significantly greater from 0.25-m or 0.5-m center plantings but was not consistently affected by site or water depth. Fertilizer enhanced propagation but not survival. After two years, Redfish Cove produced belowground biomass similar to that observed in Christmas Bay, but aboveground biomass remained significantly less. Snake Island Cove plant mortality in September 1995 may have been presaged by low root-rhizome carbohydrate levels observed in October 1994, but causes remain unknown. Further restoration of Halodule to Galveston Bay is possible at selected sites, but structural equivalency will take longer than two growing seasons to achieve.     

Ovacystis-like condition in the eastern oyster Crassostrea virginica from the northeastern Gulf of Mexico

Histological examination of the eastern oyster Crassostrea virginica from a study in Pensacola Bay, Florida, revealed 2 cases of abnormally large, basophilic ova that resembled cells characteristic of ovacystis disease previously reported in oysters from Maine and Long Island. The hypertrophied gametes measured up to 250 pm in diameter, had scant cytoplasm and contained granular nuclear-masses of Feulgen-positive material. Electron microscopy of reclaimed tissue revealed these masses to consist of virus-like particles (average 46 nm) similar to those reported in cases of ovacystis.     

Late Neogene nannofossil biostratigraphy and paleoceanography of the northeastern Gulf of Mexico and adjacent areas

Two 305 m cored sections from the northwest Florida continental shelf contain a nearly complete record of late Neogene hemipelagic sedimentation. One of the sites, south and east of De Soto Canyon, is isolated from terrigenous sediment except for sediment transported in suspension. This site contains a continuous record from the late Miocene to the Recent. The second site, on the western rim of De Soto Canyon, is more expanded and continuous from the late Pliocene to the Recent. A hiatus separates the late Pliocene from the middle Miocene. Six prominent nannofossil biohorizons were recognized within the Pleistocene, seven within the Pliocene, and three within the Miocene; in addition one biohorizon marks the base of the Pleistocene and another the base of the Pliocene.Nearly all carbonate in the sediment is pelagic. Terrigenous detrital sedimentation was controlled by glacioeustatic sea level fluctuations during the Pleistocene, and sea level changes are probably responsible for fluctuations in the ratio of pelagic carbonate to clayey detritus in pre-Pleistocene sediments also. Carbonate content, coarse fraction percent, and relative abundances of environmentally sensitive nannoplankton species suggest important paleoceanographic changes in the northeastern Gulf of Mexico and adjacent areas. Fluctuations in the relative abundance of the solution-resistant coccoliths of the genusCyclococcolithus indicate that waters at a depth of 600–1000 m were more corrosive during the late Miocene than they are today. The decrease in carbonate dissolution during the late Miocene probably was a response to gradual constriction of the Central American passage and the consequent restriction of flow of corrosive water from the Pacific. Short term fluctuations in dissolution during the Pliocene and Pleistocene are related to climatic cycles.Productivity variations in the surface waters, recorded mainly by the relative abundance of small and large morphotypes of closely related coccolith species, indicate that productivity increased during the Pliocene, but the most dramatic change — a major oceanwide increase in productivity — occurred during the Pleistocene, during and just prior to the Jaramillo magnetic event about 0.9 m.y. ago. Surprisingly the late Miocene Messinian event did not leave a significant imprint in the northeastern Gulf of Mexico.     

Molecular analyses of the diversity in marine bacterioplankton assemblages along the coastline of the northeastern Gulf of Mexico

Bacterial community diversity in marine bacterioplankton assemblages were examined in 3 coastal locations along the northeastern Gulf of Mexico (GOM) using 16S rRNA gene libraries and fluorescence in situ hybridization approaches. The majority of the sequences (30%-60%) were similar to the 16S rRNA gene sequences of unknown bacteria; however, the operational taxonomic units from members of the Cyanobacteria, Proteobacteria, and Bacteroidetes were also present at the 3 GOM sites. Overall, sequence diversity was more similar between the Gulf sites of Carrabelle and Ochlockonee than between either of the Gulf sites and Apalachicola Bay. Fluorescence in situ hybridization analyses revealed the quantitative predominance of members of the Alphaproteobacteria subclass and the Cytophaga-Flavobacterium cluster within the bacterioplankton assemblages. In general, the study further reveals the presence of many bacterial taxa that have been previously found to be dominant in coastal marine environments. Differences observed in the representation of the various bacterial phylogenetic groups among the GOM coastal sites could be partly attributed to dynamic variations in several site-specific conditions, including intermittent tidal events, nutrient availability, and anthropogenic influences.     

Acetogenic and sulfate-reducing bacteria inhabiting the rhizoplane and deep cortex cells of the sea grass Halodule wrightii.

Recent declines in sea grass distribution underscore the importance of understanding microbial community structure-function relationships in sea grass rhizospheres that might affect the viability of these plants. Phospholipid fatty acid analyses showed that sulfate-reducing bacteria and clostridia were enriched in sediments colonized by the sea grasses Halodule wrightii and Thalassia testudinum compared to an adjacent unvegetated sediment. Most-probable-number analyses found that in contrast to butyrate-producing clostridia, acetogens and acetate-utilizing sulfate reducers were enriched by an order of magnitude in rhizosphere sediments. Although sea grass roots are oxygenated in the daytime, colorimetric root incubation studies demonstrated that acetogenic O-demethylation and sulfidogenic iron precipitation activities were tightly associated with washed, sediment-free H. wrightii roots. This suggests that the associated anaerobes are able to tolerate exposure to oxygen. To localize and quantify the anaerobic microbial colonization, root thin sections were hybridized with newly developed (33)P-labeled probes that targeted (i) low-G+C-content gram-positive bacteria, (ii) cluster I species of clostridia, (iii) species of Acetobacterium, and (iv) species of Desulfovibrio. Microautoradiography revealed intercellular colonization of the roots by Acetobacterium and Desulfovibrio species. Acetogenic bacteria occurred mostly in the rhizoplane and outermost cortex cell layers, and high numbers of sulfate reducers were detected on all epidermal cells and inward, colonizing some 60% of the deepest cortex cells. Approximately 30% of epidermal cells were colonized by bacteria that hybridized with an archaeal probe, strongly suggesting the presence of methanogens. Obligate anaerobes within the roots might contribute to the vitality of sea grasses and other aquatic plants and to the biogeochemistry of the surrounding sediment.     

Seed germination in Halodule wrightii and Syringodium filiforme from Texas and the U.S. Virgin Islands

Seed germination under laboratory conditions was nearly continuous in both Halodule wrightii Aschers. and Syringodium filiforme Kütz. Halodule seeds that were sieved from sediments in Texas and St. Croix in 1978 continued to germinate for over 3 years in artificial seawater. Syringodium seeds that were collected on reproductive shoots in 1979 continued to germinate for 3 years. The potential longevity of seeds of Halodule and Syringodium may exceed that of seeds of any other seagrass.     

Observations of Lophelia pertusa and the surficial geology at a deep-water site in the northeastern Gulf of Mexico

Authigenic carbonate, precipitated in conjunction with biogeochemical activity associated with hydrocarbon and related fluid seepage, provides exposed and buried hard substrate on the crest and flanks of a low-relief mound located on the upper De Soto Slope in the northeastern Gulf of Mexico. Lophelia pertusa has successfully colonized some of this carbonate material. Individual colonies range in size from a few centimeters to over 1.5 m in diameter while aggregations of closely associated colonies with linear orientations were observed to attain 1.5–2 m in height and width and 3–4 m in length. Many of the aggregated colonies appear to be in the first phase of the `thicket' building stage described by Squires (1964). Colonies less than 50–75 cm in diameter were nearly always completely pure white. Larger colonies and the aggregated colonies are often light to dark brown in coloration at their base and center with many having only white terminal branches and some with no white corallum at all.     

The role of leaf nitrogen content in determining turtlegrass (Thalassia testudinum) grazing by a generalized herbivore in the northeastern Gulf of Mexico

In shallow marine environments the variability in grazing on seagrasses has been hypothesized to be controlled, in part, by the nutritive quality (i.e., nitrogen content) of their leaves. The few existing studies of the relationship between leaf nitrogen content and seagrass grazing have all found a positive relationship between leaf nitrogen content and preference by selective vertebrate grazers (i.e., the bucktooth parrotfish, green sea turtles, and dugongs). However, most marine herbivores (both vertebrate and invertebrate) are thought to be extreme generalists with broad diets of variable nutritive quality (e.g., detritus, living plants, and animals), suggesting the currently held view on the role leaf nutrient content in explaining the variability of seagrass grazing is an oversimplification.In this study, we evaluated how leaf nitrogen content influenced grazing on turtlegrass by a generalist invertebrate herbivore (the pink sea urchin Lytechinus variegatus) in the northeastern Gulf of Mexico. Using a short-term laboratory test and a longer-term field experiment, we tested the hypothesis that leaf nitrogen content controls sea-urchin grazing on seagrass leaves. We hypothesized that if poor nutritive value of seagrasses is responsible for reduced rates of feeding, then increasing leaf nitrogen concentrations should lead to increased rates of seagrass consumption by sea urchins.In the field experiment, we significantly enriched seagrass leaf nitrogen concentrations (some 10-20% depending on month) in experimental plots with a commercial fertilizer and we manipulated grazing intensity by enclosing adult sea urchins at densities that bracketed the range of average densities observed in the region (i.e., 0, 10 and 20 individuals/m(2)). Comparisons of changes in aboveground seagrass production and biomass showed no evidence that sea urchins grazed significantly more in treatments where leaf nitrogen was enriched. Because the statistical power of our test to detect such differences was low and aboveground seagrass production varied significantly among treatments, we also used a mass balance equation to estimate sea urchin consumption of nitrogen-enriched and unenriched leaves. This showed that sea urchins compensated for low nitrogen levels in our unenriched treatments by eating more leaves than in treatments where leaf nitrogen was elevated. Using a laboratory test, we also found that sea urchins ate less nitrogen-enriched seagrass than unenriched seagrass. In combination, these results show that, in contrast to findings reported for vertebrate herbivores, sea urchins feed at higher rates when offered seagrass leaves of lower leaf nitrogen content, and that low levels of leaf nitrogen are not always an effective defense against herbivores.     

Characterization of nitrifying, denitrifying, and overall bacterial communities in permeable marine sediments of the northeastern Gulf of Mexico

Sandy or permeable sediment deposits cover the majority of the shallow ocean seafloor, and yet the associated bacterial communities remain poorly described. The objective of this study was to expand the characterization of bacterial community diversity in permeable sediment impacted by advective pore water exchange and to assess effects of spatial, temporal, hydrodynamic, and geochemical gradients. Terminal restriction fragment length polymorphism (TRFLP) was used to analyze nearly 100 sediment samples collected from two northeastern Gulf of Mexico subtidal sites that primarily differed in their hydrodynamic conditions. Communities were described across multiple taxonomic levels using universal bacterial small subunit (SSU) rRNA targets (RNA- and DNA-based) and functional markers for nitrification (amoA) and denitrification (nosZ). Clonal analysis of SSU rRNA targets identified several taxa not previously detected in sandy sediments (i.e., Acidobacteria, Actinobacteria, Chloroflexi, Cyanobacteria, and Firmicutes). Sequence diversity was high among the overall bacterial and denitrifying communities, with members of the Alphaproteobacteria predominant in both. Diversity of bacterial nitrifiers (amoA) remained comparatively low and did not covary with the other gene targets. TRFLP fingerprinting revealed changes in sequence diversity from the family to species level across sediment depth and study site. The high diversity of facultative denitrifiers was consistent with the high permeability, deeper oxygen penetration, and high rates of aerobic respiration determined in these sediments. The high relative abundance of Gammaproteobacteria in RNA clone libraries suggests that this group may be poised to respond to short-term periodic pulses of growth substrates, and this observation warrants further investigation.     

Aspects of seagrass ecology along the western Arabian Gulf coast

Results of semi-quantitative observations and quantitative sampling of seagrasses at coastal and offshore sites along the western Arabian Gulf are presented. Overall seagrass cover (all species together) shows significant positive correlation with latitude, but not with salinity, temperature or depth. The same pattern is shown by Halodule uninervis (Forsk.) Aschers., the dominant species. Mean seagrass biomass ranged from 53–235 g m^-2 (dry weight). These values are comparable with biomass estimates from regions in which environmental conditions are generally less extreme than in the Gulf. Seagrass biomass is significantly negatively correlated with depth and sediment grain size. No significant correlation is apparent between seagrass biomass and factors such as season, salinity, or concentrations of nutrients and heavy metals measured. It is pointed out that any correlations observed are not necessarily taken to imply causality.     

Distribution of cetaceans in the offshore Gulf of Mexico

In order to comprehend better the distribution of Gulf of Mexico cetaceans, all available records of whales and dolphins in the offshore Gulf were assembled and analysed. This included sightings, strandings and captures of all species, except the Bottlenose Dolphin Tursiops truncatus , from all sources, except the recently completed GulfCet project. An attempt was made to confirm species identification for each of the records. A total of 1223 records was available for analysis. Twentyseven species of cetaceans have been confirmed to occur in the offshore Gulf of Mexico. All of the baleen whales, with the possible exception of the Bryde's Whale Balaenoptera edeni appear to be extralimital in the Gulf. The Sperm Whale Physeter macrocephalus is, by far, the most common great whale in this body of water. All previous records of Common Dolphins Delphinus spp. in the Gulf are rejected as either incorrect or unreliable, and there is currently no convincing evidence that dolphins of the genus Delphinus occur in the Gulf. The Atlantic Spotted Dolphin Stenella frontalis is the only species, other than the Bottlenose Dolphin, that regularly occurs over the continental shelf. The Pantropical Spotted Dolphin Stenella attenuata is the most common species of small cetacean in oceanic waters of the Gulf, but many other species also occur there in significant numbers.     

On the relationship between methane production and oxidation by anaerobic methanotrophic communities from cold seeps of the Gulf of Mexico

The anaerobic oxidation of methane (AOM) in the marine subsurface is a significant sink for methane in the environment, yet our understanding of its regulation and dynamics is still incomplete. Relatively few groups of microorganisms consume methane in subsurface environments – namely the anaerobic methanotrophic archaea (ANME clades 1, 2 and 3), which are phylogenetically related to methanogenic archaea. Anaerobic oxidation of methane presumably proceeds via a 'reversed' methanogenic pathway. The ANME are generally associated with sulfate-reducing bacteria (SRB) and sulfate is the only documented final electron acceptor for AOM in marine sediments. Our comparative study explored the coupling of AOM with sulfate reduction (SR) and methane generation (MOG) in microbial communities from Gulf of Mexico cold seep sediments that were naturally enriched with methane and other hydrocarbons. These sediments harbour a variety of ANME clades and SRB. Following enrichment under an atmosphere of methane, AOM fuelled 50–100% of SR, even in sediment slurries containing petroleum-associated hydrocarbons and organic matter. In the presence of methane and sulfate, the investigated microbial communities produce methane at a small fraction (∼10%) of the AOM rate. Anaerobic oxidation of methane, MOG and SR rates decreased significantly with decreasing concentration of methane, and in the presence of the SR inhibitor molybdate, but reacted differently to the MOG inhibitor 2-bromoethanesulfonate (BES). The addition of acetate, a possible breakdown product of petroleum in situ and a potential intermediate in AOM/SR syntrophy, did not suppress AOM activity; rather acetate stimulated microbial activity in oily sediment slurries.     

Histopathologic survey of ovaries of fish from petroleum production and control sites in the Gulf of Mexico

Ovaries collected from 11 species of demersal and platform-associated pelagic fishes were examined for petroleum induced lesions. The fish were taken from the north central Gulf of Mexico near 20 oil or gas production platforms and three control sites during two periods, spring and late summer. The status of the ovarian cycle correlated well in the majority of cases with known spawning times. Histopathological conditions most prominent in the ovary other than atresia, generally considered physiological, were acidophilic granular cells, chromatophores and leucocytic foci or infiltration, whereas degeneration, helminths and protozoa with associated tissue responses were rarely noted. Minimal morphological evidence of adverse effects by toxic petroleum elements on the ovary was found., Accepted 21 July 1980     

Histopathological survey of male gonads offish from petroleum production and control sites in the Gulf of Mexico

Testes, collected from 11 species of demersal and platform associated pelagic fishes, were examined microscopically for petroleum induced lesions. The fish were taken from 19 oil and gas production platforms and four control sites from the north-central Gulf of Mexico, during two periods, spring and late summer. The status of the spermatogenic cycle at the time specimens were collected correlated well with the known spawning times for the respective species of fish with one exception. Histopathological evaluation revealed acidophilic granular cells, chromatophores, degeneration and focal leucocytic aggregations as the most prominent and abundant conditions in testes, with encapsulated cysts, protozoa and helminths being observed only rarely. No evidence of adverse effects on gonadal morphology was found.     

An enigmatic heteronemertean from the Gulf of Mexico

Heteroenopleus enigmaticus gen. nov., sp. nov. isdescribed from the northwestern Gulf of Mexico. Itoccurs both in the nearshore Gulf and in high salinityestuarine areas. Body wall musculature conforms tothe general plan of heteronemerteans, i.e. outerlongitudinal, middle circular and inner longitudinalmuscle layers. The head bears a pair of horizontallateral slits, characteristic of the lineids. Theproboscis musculature is of the heteronemerteanpalaeotype with two muscle layers, outer circularand inner longitudinal. Other morphologicalcharacters agree with those of heteronemerteans withone major exception: the proboscis is armed with tworows of structures, herein named parastylets. Thisarmature has no morphological similarity to either themicrovillar proboscideal spines of heteronemerteans orto the stylets of mono- or polystiliferans. A newsubfamily Heteroenopleinae is erected to contain theapomorphic character of an armed proboscis.     

Habitat use by Mountain Plovers in prairie dog colonies in northeastern New Mexico

ABSTRACT Mountain Plovers (Charadrius montanus) are grassland birds that often breed in close association with colonies of black‐tailed prairie dogs (Cynomys ludovicianus). However, not all colonies provide plover nesting habitat or habitat of equal quality, and the characteristics of colonies important for plovers remain poorly understood. Over two years, I used plover distribution surveys, territory mapping, and habitat sampling to study habitat use by plovers in prairie dog colonies in shortgrass prairie in northeastern New Mexico. My objective was to document important components of plover breeding habitat in colonies by comparing characteristics of used and unused habitats at three spatial scales: colony, territory, and nest‐site. I found evidence of plover breeding in 14 of 44 colonies in 2009 and 13 of 43 colonies in 2010. Based on logistic regression, the probability of a colony being occupied by plovers was positively associated with colony size, but negatively associated with mean vegetation height. Preference for larger colonies could relate to minimum habitat requirements, or a potential tendency of this species to nest in social clusters. Shorter vegetation height was strongly correlated with greater bare ground and lower forb/subshrub cover, all characteristics that may be related to plover predator avoidance and foraging microhabitat. At both the territory and nest‐site scale, areas used by plovers had shorter vegetation, more bare ground, and less forb/subshrub cover than unused areas. Nest sites were also more sloped, perhaps to reduce risk of flooding, and located further away from the nearest prairie dog burrow, perhaps to reduce risk of disturbance. Overall, my results show that plover use of prairie dog colonies was influenced by landscape and habitat features of colonies, and suggest that large colonies are particularly valuable because they are most likely to contain adequate areas with preferred habitat characteristics.     

Blood parasites of some birds from northeastern Mexico

A total of 196 birds of 31 species from 15 families from northeastern Mexico was examined for blood parasites; 25 birds (12.8%) of 11 species harbored 1 or more species of hematozoans. Species of Haemoproteus accounted for half of the total infections encountered. Leucocytozoon simondi was found in 2 Mexico ducks (Anas diazi) and this represents the first record of the transmission of this parasite in Mexico. The results of this survey were compared with those obtained nearly 50 yr ago from a survey of birds from the same general area; prevalence in both samples was similar, despite the change to a more agricultural environment over this period.     

Oceanography of the Gulf of Tehuantepec, Mexico, indicated by Radiolaria remains

The morphology of the Gulf of Tehuantepec, Mexico, is redefined through a bathymetric analysis and this redifinition is considered to explain the distribution patterns of non-carbonate sediments, particularly the radiolarian remains. Although the distribution patterns of pelagic sediments are influenced by the Gulf of Tehuantepec bathymetrical morphology and the terrigenous input, these “reflect” clearly the regional oceanography. Northern strong winds, called the “Tehuanos”, blowing across the sea surface of this Gulf when the Intertropical Convergence is at its southernmost position, define the main sedimentologic dynamics of this region. This wind field gives rise to an oceanic front at the southwestern part of the Gulf, as well as upwelling processes at its central-eastern part. In the mixing at the front there is some contribution of California Current waters, while in the upwelling area there is a large contribution of the tropical-subtropical water which is carried by the Costa Rica Coastal Current. This oceanographic framework controlls the makeup of radiolarian assemblages. Consequently, at the seabottom, under the oceanic front, the presence of the radiolarian species Lithomelissa thoracites and Cycladophora davisiana is observed, while under the upwelling region, Acrosphaera murrayana is conspicuous.