Cell adsorption and selective desorption for separation of microbial cells by using chitosan-immobilized silica

Cell adsorption and selective desorption for separation of microbial cells were conducted by using chitosan-immobilized silica (CIS). When chitosan was immobilized onto silica surfaces with glutaraldehyde, bacterial cells adsorbed well and retained viability. Testing of the adsorption and desorption ability of CIS using various microbes such as Escherichia coli, Aeromonas hydrophila, Pseudomonas aeruginosa, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus casei, Streptococcus mutans, Streptococcus sobrinus, Streptococcus salivarius, Saccharomyces cerevisiae, Saccharomyces ludwigii, and Schizosaccharomyces pombe revealed that most microbes could be adsorbed and selectively desorbed under different conditions. In particular, recovery was improved when L-cysteine was added. A mixture of two bacterial strains adsorbed onto CIS could also be successfully separated by use of specific solutions for each strain. Most of the desorbed cells were alive. Thus, quantitative and selective fractionation of cells is readily achievable by employing chitosan, a known antibacterial material.     

Ethanol separation from molasses based fermentation broth by reverse osmosis

Summary Irradiated styrene-grafted cellulose acetate membrane was used for the separation of ethanol by reverse osmosis. Ethanol separation from molasses based fermentation broth resulted in separation efficiency of 90% at an operating pressure of 1400 psig. Lower permeate flux was observed with fermented broth compared to aqueous ethanol.     

Thermodynamics of water and ethanol adsorption on four starches as model biomass separation systems

Adsorption of water and ethanol on four starches has been studied in the temperature range of 50-90 degrees C. The results show that adsorption of water on starch-based materials is enhanced when the amount of amylopectin is highest. Adsorption of ethanol is not significantly affected by the kind of starch used. Heats of adsorption calculated from retention data are in the range from -9.3 to -13.7 kcal/g mol for water and -5.6 to -6.76 kcal/g mol for ethanol. Calculated free energies of adsorption suggest that adsorption is most spontaneous at lower temperatures as expected.     

Advances in water treatment by adsorption technology

Among various water purification and recycling technologies, adsorption is a fast, inexpensive and universal method. The development of low-cost adsorbents has led to the rapid growth of research interests in this field. The present protocol describes salient features of adsorption and details experimental methodologies for the development and characterization of low-cost adsorbents, water treatment and recycling using adsorption technology including batch processes and column operations. The protocol describes the development of inexpensive adsorbents from waste materials, which takes only 1-2 days, and an adsorption process taking 15-120 min for the removal of pollutants. The applications of batch and column processes are discussed, along with suggestions to make this technology more popular and applicable.     

Dynamic behavior and selective adsorption of a methanol/water mixture inside a cyclic peptide nanotube

Present molecular dynamics simulations indicate that the methanol component in a methanol/water mixture is more likely to be trapped in a cyclic peptide nanotube (CPNT), while water molecules tend to be present at the channel mouths as transient guests. Channel water resides mainly between methanol and the CPNT wall, resulting in a distinct decrease in the H-bond number per channel methanol. Six designed CPNTs with different channel diameters and outer surface characteristics all possess distinct selectivity to methanol over water. Of these, the amphipathic 8?×?(AQ)₄-CPNT exhibits the best performance. Results in this study provide basic information for the application of a CPNT to enrich methanol from a methanol/water mixture.

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Graphical Abstract Typical overview of water and methanol molecular distribution in cyclic peptide nanotubes

     

Supercoiled plasmid DNA: selective purification by thiophilic/aromatic adsorption

Separation of the different plasmid isoforms is a major challenge in purifying plasmid DNA. We describe a new type of biochemical interaction that occurs in the presence of high concentrations of lyotropic salt and results in the selective adsorption of supercoiled plasmid DNA to aromatic thioether ligands. Under well-defined conditions, these ligands are capable of separating supercoiled plasmid DNA (ccc) from its isoform, i.e. open circular (oc) form. Integrated in a process, preceded by group separation and followed by anion-exchange chromatography, this new purification method may facilitate the production of highly purified supercoiled plasmid DNA for use in gene therapy and DNA vaccine applications.     

Plasmid DNA purification by selective calcium silicate adsorption of closely related impurities

The selective adsorption of supercoiled plasmid, open-circular plasmid, and genomic DNA to gyrolite, a compound from the class of crystalline calcium silicate hydrates, is investigated and exploited for purification purposes. Genomic DNA and open-circular plasmid bind to gyrolite adsorbents with greater affinity than the more conformationally constrained supercoiled plasmid. As such, the gyrolite adsorbents are an economical and scaleable alternative to chromatographic purification for the removal of DNA impurities from solutions containing supercoiled plasmid. The advantage of gyrolite adsorbents is their lower unit price and ability to selectively adsorb DNA impurities without binding supercoiled plasmid under certain conditions. The effects of ionic strength, temperature, chelating agent, divalent cation, and lyotropic salts on adsorption of highly purified plasmid are studied to understand the forces that bind DNA to gyrolite, a structure with hydrophilic and hydrophobic characteristics. The results indicate that DNA binding is governed by hydrogen bonding, electrostatic bridging with divalent cations, shielding of electrostatic repulsion, hydrophobic adsorption, and disruption of integral surface water layer on gyrolite. On the basis of results from a range of Hofmeister series salts, strongly hydrated anions may enhance DNA adsorption by promoting hydrophobic interactions between DNA and gyrolite. Conversely, the very weakly hydrated chaotrope I(-) may enhance adsorption by strongly associating with hydrophobic siloxanes of gyrolite, thereby disrupting an integral water layer, which competes for hydrogen bonding sites.     

Electrophorus electricus acetylcholinesterases; separation and selective modification by collagenase.

Abstract— The multiple forms of acetylcholinesterase found in the electric organ of the eel Electrophorus electricus have been fractionated by differential solubilization from an ammonium sulfate precipitate by means of a column elution procedure (King , 1972). This procedure cleanly separates ‘native’ forms from ‘degraded’ forms, and subsequent sedimentation reveals three native and two degraded forms. All three native forms, in distinction to the degraded ones, are insoluble at low ionic strength and are shifted to higher sedimentation constants by limited collagenase treatment. These results suggest that the long (500 Á) tail seen previously on the native forms of this enzyme (Dudai et al., 1973; Rieger et al., 1973a, b) may contain collagen.     

Small molecular ion adsorption on proteins and DNAs revealed by separation techniques

Ion binding is a term that assumes that the ion is included in the solvation sphere characterising the biomolecule. The binding forces are not clearly stated except for electrostatic attraction; weak forces (hydrogen bonds and Van der Waals forces) are likely involved. Many publications have dealt with ion binding to proteins and the consequences over the past 10 years, but only a few studies were performed using high-performance liquid chromatography (HPLC: ion exchange, reversed phase without the well-identified immobilised metal affinity chromatography) and capillary zone electrophoresis (CZE). This review focuses on the binding of proteins and DNAs mainly to the oxyanions (phosphate, borate, citrate) and amines used as buffers for both the HPLC eluent and the background electrolyte of CZE. Such specific ion adsorption on biomolecules is evidenced by physico-chemical characteristics such as the mobility or retention volume, closely associated with the net charge, which differ from the expected or experimental data obtained under the conditions of an indifferent electrolyte. It is shown that ion binding to proteins is a key parameter in the electrostatic repulsion between the free protein and a fouled membrane in the ultrafiltration separation of a protein mixture.     

免疫磁珠富集技术联合选择性培养基快速检测单增李斯特菌

单增李斯特菌是一种危害极大的食源性致病菌,建立快速及特异的检测方法对于食品安全监控尤为重要。文中联合免疫磁珠与选择性培养基对不同浓度(101~105CFU/mL)单增李斯特菌进行检测,并对3种李斯特菌、金黄色葡萄球菌及副溶血弧菌进行交叉试验;同时模拟食物污染,探索免疫磁珠-平板法检测样品的检测限以及该方法的最快检测时间。结果显示特异性免疫磁珠联合选择性平板法可检出浓度为103CFU/mL及以上的单增李斯特菌;牛奶样品仅需6 h增菌能被检出,检测限为0.7 CFU/mL。联合使用免疫磁珠富集技术与选择性培养基,能在30 h内完成对牛奶样品的检测,较国标法减少38 h以上,且具有同等的灵敏度。     

Assay of N-myristoyl transferase by selective adsorption of myristoyl-coenzyme A on acidic alumina

We have developed a simple and rapid method for detecting the enzyme myristoyl-CoA:protein N-myristoyl transferase. The enzyme catalyzes the transfer of the myristoyl moiety of myristoyl-CoA to the amino-terminal glycine residue of a peptide (protein). Incorporation of the [14C]myristate into the peptide is quantified after separation of the [14C]myristoyl-peptide from unreacted [14C]myristoyl-CoA by selective adsorption of [14C]myristoyl-CoA on acidic alumina. Optimal assay concentrations were 200 microM synthetic peptide, 1 microM [14C]myristoyl-CoA, 10 mM Tris-HCl/1 mM dithiothreitol/0.1 mM ethylene glycol bis(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid/aprotinin (10 micrograms/ml) buffer, pH 7.4, and 1-10 micrograms protein.     

The selective adsorption of polysaccharides on polyaromatic surfaces

A new matrix, polymeric 1,3-diaminobenzene-coated Celite, which selectively adsorbs monomeric and polymeric carbohydrates, has been prepared. This matrix adsorbs glycogen and other branched polysaccharides, as opposed to neutral or charged monosaccharides. Other solid supports were coated with polymeric 1,3-diaminobenzene; some of these (coated Sephadex G10 and coated Bioglas 1,000) had better physical form for column packing. Coated Celite was considered to be the best support in view of its greater stability. The effects of ionic concentration, pH, temperature, and the concentration of carbohydrate solution on the adsorption of glycogen on to coated Celite were studied, and methods to prevent adsorption and remove adsorbed carbohydrate were investigated. A comparison is made with the adsorption of heterocyclic compounds by cross-linked dextran gels.