共查询到20条相似文献,搜索用时 31 毫秒
1.
Ajith Anand C. Srinivasa Rao R. Latha P. C. Josekutty P. Balakrishna 《In vitro cellular & developmental biology. Plant》1998,34(2):136-140
Summary Micropropagation ofUraria picta, a leguminous herb, was achieved through axillary bud culture and nodal callus culture. Bud break was best when nodes were
cultured on Murashige and Skoog (1962) (MS) medium supplemented with 2.6 μM α-naphthalene acetic acid and 4.4 μM N6-benzyladenine. Optimum shoot multiplication was observed in adenine sulphate at 2.47 μM concentration. Competent callus was initiated around the nodal ring of the explant on the basal medium supplemented with
cytokinins and auxin (α-naphthalene acetic acid and N6-benzyladenine), which regenerated into new profusely growing shoots on transferring to 0.13 μM N6-benzyladenine. Shoots elongated to 5 node length with 1.11 μM N6-benzyladenine were rooted on half-strength MS basal medium. The rooted plants were successfully established with 80% survival.
About 400 such plants were transferred to the field. 相似文献
2.
Thidiazuron Induced Multiple Shoot Induction and Plant Regeneration from Cotyledonary Explants of Mulberry 总被引:4,自引:0,他引:4
T. Dennis Thomas 《Biologia Plantarum》2003,46(4):529-533
A rapid micropropagation protocol through induced multiple shoots from the cotyledonary explant of mulberry (Morus alba L) is described. The highest number of shoots (20.3) was obtained when explants from 14-d-old embryos were cultured on Murashige
and Skoog (MS) medium supplemented with 7 μM thidiazuron for 45 d. Of the three cultivars used, cv. S-36 was the best followed
by cv. K-2 and S-1. The shoots were transferred to MS medium supplemented with 5 μM 6-benzylaminopurine for elongation. The
elongated shoots were rooted on half strength MS medium containing 1 – 7 μM indole 3-butyric acid or 1-naphthalene acetic
acid. The rooted plants were transplanted to soil with 90 % success. The emerged shoot primordia probably initiated from the
pre-existing meristems since the shoot bud show definite vascular connection to the major vascular tissue.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
3.
Marie-Claire D'Souza Madhuri Sharon 《In vitro cellular & developmental biology. Plant》2001,37(2):168-172
Summary A protocol for in vitro propagation of Bixa orellana is described. Plants were regenerated from shoot apex and nodal explants on B5 medium supplemented with 4.9 μM 2-isopentenyl adenine. The multiplication factor of shoot apex explants was higher (nine shoots per explant) than that of
the nodal explants (five shoots per explant). Regardless of the position of the nodes, all the nodal explants gave similar
responses. However, the size of the nodal explant was an important factor in producing multiple shoots: 0.5 cm nodal explants
produced the maximum multiple shoots. Regenerated shoots from shoot apex explants rooted best on MS medium supplemented with
0.05 μM α-naphthalene acetic acid (NAA). whereas shoots regenerated from nodal explants needed 2.7 μM NAA for rooting. Eighty per cent survival of in vivo transferred plants occurred on the best potting substrate, coco peat. Since the multiplication factor was nine per explant,
this protocol can be use for commercial microprogation. However, the regeneration capacity declined after 10 subcultures.
Approximately, 3350 rooted plants could be generated in 10 mo. after eight subcultures, from one shoot with a shoot apex and
four nodes. 相似文献
4.
An efficient regeneration system for large-scale propagation of statice (Limonium altaica cv. Emille) was developed using leaves from mature plants. Leaf segments (5×5 mm sections) were cultured on Murashige and
Skoog's medium supplemented with N6-benzyladenine (BA) and thidiazuron (TDZ) individually and in combination with indole-3-acetic acid (IAA) and α-naphthaleneacetic
acid (NAA). Prolific direct adventitious shoot regeneration occurred on most of the media. The best response in terms of frequency
of shoot regeneration (99.5%) and number of shoots per explant (112 shoots per explant) was observed on medium supplemented
with 2.85 μM IAA and 1.14 μM TDZ. The shoots rooted easily on half strength MS medium and MS medium with indole-3-butyric
acid. In vitro propagated plants could be transferred to soil with survival rates of more than 95%.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
5.
An efficient plant regeneration system for Mucuna pruriens L. (DC.) using cotyledonary node explants
Summary The purpose of this study was to develop an efficient micropropagation system for Mucuna pruriens, an important medicinal plant in India. A range of cytokinins was investigated for multiple shoot regeneration with cotyledonary
node explants from 7-d-old aseptic seedlings. Of all the cytokinins, 6-benzyladenine (BA), kinetin (KIN) and 2-isopentenyl
adenine (2-iP) tested in Murashige and Skoog medium (MS), BA was the most effective and 5.0 μM was found to be optimum for inducing maximum shoots. Medium types, medium strength and pH were also investigated for induction
and proliferation of shoots. The highest efficiency of shoot proliferation was observed in 5.0 μM BA and 0.5 μM α-naphthalene acetic acid (NAA) in half-strength MS medium at pH 5.8. The best condition for rooting was half-strength MS
medium solidified with agar and with 2.0 μM indole-3-butyric acid (IBA). After rooting, the plantlets were transferred to plastic pots filled with sterile soilrite where
90% grew and all exhibited normal development. 相似文献
6.
Summary An efficient in vitro propagation system was developed for Arnebia euchroma, an important Chinese traditional medicinal plant. The present study utilized thidiazuron (TDZ) for the induction of shoot
organogenesis on cotyledon and hypocotyl explants. The maximal number of shoots was obtained on the modified Linsmaier and
Skoog (LS) medium supplemented with 1.0 mgl−1 (4.5 μM) TDZ for 12d on cotyledon explants (8.6 shoots per cotyledon explant). Other cytokinins (kinetin and 6-benzyladenine) and
auxin (α-naphthaleneacetic acid) were not efficient in inducing regeneration on cotyledon explants. Browning of the basal
portion of the subcultured shoots could be significantly reduced when they were cultured on the modified LS medium supplemented
with 100 mgl−1 (33.3 μM) polyvinylpyrrolidone. Well-developed shoots formed roots on the same medium containing 1.0 mgl−1 (4.9 μM) indole-3-butyric acid. The efficient regeneration protocol reported here provides an important means of micropropagation
of this plant. Furthermore, this protocol is essential to future genetic improvement of plants via transformation protocols. 相似文献
7.
An efficient protocol is described for the rapid in vitro multiplication of an endangered medicinal plant, Tylophora indica (Burm. f.) Merrill, via enhanced axillary bud proliferation from nodal explants collected from young shoots of a two-year-old
plant. The physiological effects of growth regulators [6-benzyladenine (BA), kinetin (Kin) thidiazuron (TDZ), indole-3-acetic
acid (IAA), indole-3-butyric acid (IBA) or α-naphthalene acetic acid (NAA)], ascorbic acid (AA), different strengths of Murashige
and Skoog (MS) medium and various pH levels on in vitro morphogenesis were investigated. The highest number (8.6 ± 0.71) of
shoots and the maximum average shoot length (5.2 ± 0.31 cm) were recorded on MS medium supplemented with 2.5 μM BA, 0.5 μM
NAA and 100 mg/l AA at pH 5.8. Rooting was best achieved on half-strength MS medium augmented with 0.5 μM IBA. The plantlets
regenerated in vitro with well-developed shoot and roots were successfully established in pots containing garden soil and
grown in a greenhouse with a 90% survival rate. The regenerated plants did not show any immediate detectable phenotypic variation.
The described method can be successfully employed for large-scale multiplication and long-term in vitro conservation of T. indica. 相似文献
8.
H. Y. Seo Y. J. Kim T. I. Park H. S. Kim S. J. Yun K. H. Park M. K. Oh M. Y. Choi C. H. Paik Y. S. Lee Y. E. Choi 《In vitro cellular & developmental biology. Plant》2007,43(3):209-214
Sesamum indicum L. was used as an important oil crop in the world. An efficient protocol for in vitro plant regeneration via adventitious shoot formation from deembryonated cotyledon explants isolated from mature seeds of sesame
is developed. Optimal medium for direct adventitious shoot formation was Murashige and Skoog (MS) medium with 22.2 μM 6-benzylaminopurin
(BA) and 5.7 μM indole-3-acetic acid (IAA). Abscisic acid (3.8 μM ABA) and AgNO3 (29.4 μM) were effective in enhancing the frequency of adventitious shoot formation. Preculture of cotyledon explants on
high sucrose concentration (6–9%) for 2 wk and subsequent transfer to 3% sucrose enhanced the frequency of adventitious shoot
induction. Root formation from the adventitious shoots was easily achieved on MS medium containing 2.7 μM of α-naphthalene
acetic acid (NAA). Regenerated plantlets were acclimatized on sand and peat moss (1:1), showing 95% survival with subsequent
flowering and seed set. We established the high-frequency plant regeneration via adventitious shoot formation in S. indicum L. 相似文献
9.
Sancak Cengiz Mirici Semra Özcan Sebahattin 《Plant Cell, Tissue and Organ Culture》2000,61(3):231-235
An in vitro propagation protocol has been developed from mature trees of Pittosporum napaulensis. The best bud proliferation (83.1%), shoot number (21 axillary shoots/ explant) and shoot length (5.5 cm) was achieved in
Murashige and Skoog (MS) medium supplemented with 5.0 μM N−6 benzyladenine and 0.1 μM α- naphthalene acetic acid. Of the three cytokinins tested (N−6 benzyladenine, kinetin and thidiazuron), N−6 benzyladenine proved to be the best for shoot induction. Shoot regeneration potential varied among genotypes. Regenerated
shoots rooted after 48 hours treatment on half-strength MS liquid medium supplemented with 20 μM indole-3-butyric acid. Rooted
shoots transferred to 120 g (w/v) soilrite + sand + soil (1:1:1) mixture showed 70% survival. Twenty-one plantlets are growing
well in green house conditions.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
10.
Murugesan Dhandapani Doo Hwan Kim Seung-Beom Hong 《In vitro cellular & developmental biology. Plant》2008,44(1):18-25
High-frequency plant regeneration of C. roseus cv. ‘little bright eye’ via somatic embryogenesis and organogenesis from five out of six explants was standardized. Two factors
were found to be important for regeneration: (1) the type of explants, and (2) the combination and concentrations of plant
growth regulators. The highest regeneration percentage through somatic embryogenesis was obtained from mature zygotic embryo
in MS medium supplemented with 7.5 μM of thidiazuron (TDZ). The mature embryo also regenerated efficiently via organogenesis
in MS medium supplemented with either 2.5 μM TDZ or 5.3 μM α-naphthalene acetic acid (NAA) and 2.2 μM 6-benzylaminopurine
(BA). Hypocotyl and cotyledon did not induce somatic embryogenesis and organogenesis in TDZ-containing medium but gave a maximum
percentage of shoots in MS medium supplemented with 5.3 μM NAA and 2.2 μM BA. Stem nodes and meristem tips showed better regeneration
via organogenesis in the medium supplemented with NAA and BA and in lower concentrations of TDZ. 相似文献
11.
Stimulation of in vitro shoot proliferation from nodal explants of cassava by thidiazuron, benzyladenine and gibberellic acid 总被引:2,自引:0,他引:2
Basdeo Bhagwat Luiz G. F. Vieiral Larry R. Erickson 《Plant Cell, Tissue and Organ Culture》1996,46(1):1-7
Multiple shoots were produced from nodal explants of cassava (Manihot esculenta Crantz) by a two-step procedure: a 6- to 8-day exposure to 0.11–0.22 µM thidiazuron (TDZ) in liquid Murashige and Skoog (MS) medium followed by culture on agar-solidified MS medium supplemented with 2.2 µM 6-benzyladenine (BA) and 1.6 M gibberellic acid (GA3). TDZ caused the nodal explants to expand and this expansion (growth) continued during culture with BA and GA3. From this expanded explant, clusters of buds and fasciated stems developed continuously and these gave rise to shoots. The shoot proliferation process was open-ended, yielding an average of 31.5 shoots per nodal explant after 10 weeks of culture with genotype CG 1–56. A positive response was also obtained from seven other genotypes evaluated with this protocol.Abbreviations BA
6-benzyladenine
- BM
basal medium
- DPU
1,3-diphenylurea
- GA3
gibberellie acid
- 2iP
isopentenyladenine
- MSM
multiple shoot medium
- NAA
1-naphthaleneacetic acid
- PGR
plant growth regulator
- TDZ
thidiazuron
- Z
zeatin 相似文献
12.
A micro-propagating system based on the young stem node segments of Sophora flavescens Ait. (Fabaceae) was established. Murashige and Skoog (MS) basal medium supplemented with 8.88 μM 6-benzyladenine (BA) plus 2.69 μM α-naphthalene
acetic acid (NAA) and that with only 5.37 μM NAA were found the best in promoting proliferation of shoots and induction of
root, respectively. Percentages of shoot induction and number of shoot per explant were up to 93.4 % and 4.2 and rooting rate
to 82.4 %, respectively. The segments of the regenerated shoots could be continuously induced to reproduce new shoots through
subculture on the same medium in 30-d intervals and still kept this activity after being subcultured for 6 generations. After
the regenerated plantlets were transplanted to field, they grew well, showing no any visible abnormalities.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
13.
Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from gerbera petioles 总被引:4,自引:0,他引:4
Orlikowska Teresa Nowak Elzbieta Marasek Agnieszka Kucharska Danuta 《Plant Cell, Tissue and Organ Culture》1999,59(2):95-102
An effective system for in vitro regeneration of adventitious shoots from callus for the transformation or mutation of gerbera was developed. Callus was produced
from petioles of the youngest 3–4 leaves detached from auxillary shoots produced in vitro. Induction medium, on which leaves were incubated over 3 or 6 days, contained 2.3 μM thidiazuron and 0.53 μM α-naphthaleneacetic
acid. Explants were than transferred to one of three regeneration media with lower levels of growth regulators. Regeneration
was quantified over four (4-weeks each) passages at the time of explant transfer to fresh medium. Direct shoot regeneration
occurred during the first 4 weeks, and after these shoots were discarded a semi-compact organogenic callus was produced. Effectiveness
of shoot regeneration depended on four criteria: the cultivar (three cultivars were tested), the sequence of passage on regeneration
medium, the growth regulators in regeneration medium and the duration of the induction period. Regeneration potential from
calli of all cultivars increased from the first to the fourth passage. Duration of the incubation period on induction medium
(3 or 6 days) influenced regeneration to varying degrees, depending on the cultivar used and the regeneration medium contents.
There were no differences between two of the regeneration media – B, containing 2.2 μM 6-benzyladenine and 0.3 μM indole-3-acetic
acid and C, containing 4.4 μM 6-benzyladenine, 4.6 μM zeatin and 0.6 μM indole-3-acetic acid. Cultivar Mariola was the most
productive and regenerated more than seven shoots per callus in the fourth passage. Regeneration on medium B was further evaluated
on four additional gerbera cultivars.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
14.
Cao Dinh Hung Krystyna Johnson Fraser Torpy 《In vitro cellular & developmental biology. Plant》2006,42(6):548-552
Summary An efficient protocol for in vitro propagation of the valuable medicinal plant, Wasabia japonica (Miq.) Matsumura is described through shoot tip proliferation and direct regeneration. Multiple shoots were induced from
shoort tips cultured on Murashige and Skoog (MS) semi-solid medium containing various concentrations (0.5–50 μM) of N6-benzyladenine (BA), thidiazuron, kinetin, and zeatin. A comparison was made on shoot multiplication between semi-solid and
liquid culture media. Well-developed shoots were obtained using full-strength MS semi-solid medium containing 5.0 μM BA. However, the greatest shoot proliferation was achieved on either full- or half-strength MS liquid media supplemented
with 5.0 μM BA for 4 wk (15.3±0.9 and 15.0±0.7 shoots per explant, respectively), and on half-strength MS liquid medium for 6 wk (25.8±1.3
shoots per explant) in culture. In contrast, the maximum number of shoots per explant on full-strength MS semi-solid medium
was achieved with either 5.0 μM BA (10.4±0.6 shoots per explant) or 10.0 μM kinetin (10.9±0.8 shoots per explant). Fresh weight of explants and length of shoots derived from full-strength MS liquid
medium (1055±77 mg and 34.2±1.0 mm, respectively) were significantly higher than those derived from full-strength MS semisolid
medium (437.6±17.3 mg and 15.4±0.7 mm, respectively). Quarter-strength MS liquid medium had no significant difference in shoot
proliferation when compared to quarter-strength MS semi-solid medium. Elongated shoots were separated and rooted on half-strength
MS semi-solid media fortified with 1-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), or indole-3-acetic acid (IAA)
ranging from 0.1 to 10.0 μM. Root formation was greatest with IBA when compared with IAA and NAA. One hundred percent of shoots were rooted on half-strength
MS medium with 5.0 μM IBA, while vigorous roots were obtained with 10.0 μM IBA. Micropropagated plantlets were successfully established in soil with 95% survival rate after heardening. 相似文献
15.
Ajith Anand Chaluvadi Srinivasa Rao 《In vitro cellular & developmental biology. Plant》2000,36(1):61-64
Summary A protocol is described for rapid multiplication of Piper barberi Gamble (Piperaceae) through shoot tip and nodal explant cultures. Nodal explants with a single axillary meristem showed three
times better response with respect to shoot proliferation when compared to shoot tip explants. The best shoot proliferation
response of nodal explants was observed with a cytokinin combination of N6-benzyladenine (4.43 μM) and kinetin (2.32 μM), with 88% bud break. The number of shoot initials (2.4) produced per nodal explant was twice the number of shoot initials
(1.2) per shoot tip. An average of 6.9±0.58 adventitious shoots were observed from the proximal end of the internodal explants
on Mursashige and Skoog (1962) (Ms) basal medium supplemented with N6-benzyladenine (2.22 μM) and kinetin (0.46 μM). A multiplication rate of 82 shoots per explant could be achieved after 9 wk of subculturing. The in vitro shoots were rooted on one-half and one-quarter MS basal medium. The shoots rooted on one-quarter MS in the dark produced
eight roots with an average root length of 3.36 cm and 98% survival. These plants were transferred to the field with a survival
rate of 75%. 相似文献
16.
Pretreatment in thidiazuron improves the in vitro shoot induction from leaves in Curculigo orchioides Gaertn., an endangered medicinal plant 总被引:1,自引:0,他引:1
T. Dennis Thomas 《Acta Physiologiae Plantarum》2007,29(5):455-461
Leaf regeneration via direct induction of adventitious shoots obtained from an endangered medicinal plant, Curculigo orchioides Gaertn. by pretreating with thidiazuron. C. orchioides is an endangered medicinal herb belonging to the family Hypoxidaceae. Direct inoculation of leaf pieces on MS medium supplemented
with various concentrations of BAP (2–8 μM) or TDZ (2–8 μM) alone or in combination with NAA (0.5 and 1.0 μM) produced low
shoot induction both in terms of % response and number of shoots per explant. Hence, leaf explants were pretreated with 15,
25 or 50 μM thidiazuron (TDZ), for 6, 24 or 48 h with the aim of improving shoot regeneration from cultured explants. After
pretreatment, explants were transferred to an agar solidified MS medium that was supplemented with BAP (4 μM), TDZ (6 μM),
BAP (4 μM) + NAA (1.0 μM), TDZ (6 μM) + NAA (0.5 μM). Control explants were incubated directly on the medium without any pretreatment.
The pretreatment of explants with 15 μM TDZ for 24 h significantly promoted the formation of adventitious shoots and the maximum
response was observed on MS medium supplemented with 6 μM TDZ. In this medium, 96 % cultures responded with an average number
of 16.2 adventitious shoots per explant. The percentage of leaf explants producing shoots and the average number of shoots
per explant were significantly improved when TDZ pretreated leaves were cultured onto MS medium supplemented with BAP or TDZ
alone or in combination with NAA. The rooted plantlets were successfully transplanted to soil with 90% success. The present
investigation indicated the stimulatory role of TDZ pretreatment in regulating shoot regeneration from leaf explants of C. orchioides. 相似文献
17.
Pradeep K. Agarwal Rajinder S. Ranu 《In vitro cellular & developmental biology. Plant》2000,36(5):392-397
Summary The in vitro plant regeneration potential of vegetatively propagated geraniums (Pelargonium x hortorum) has been investigated. Using various combinations of growth regulators and a choice of different explants, a regeneration
protocol has been developed to raise in vitro plantlets from young petiole and leaf explants from three different cultivars of geraniums. In all three cultivars, very
young petiole explants exhibited a higher regeneration potential as compared with leaf explants. Regeneration efficiencies
were found to be highly dependent on the cultivar, with cv. Samba showing the highest regeneration potential, followed by
cvs. Yours Truly and then Sincerity. Samba also showed the highest number of shoots from both the petiole [57 shoot buds per
petiole explant in the presence of 3 μM zeatin and 1 μM indole-3-acetic acid (IAA) and leaf explants (43 shoots per leaf explant with 10 μM zeatin and 2 μM IAA). Shoot buds transferred to Murashige and Skoog (MS) medium supplemented with 0.44 μM N6-benzyladenine and 0.11 μM IAA grew vigorously and attained 1–2 cm in length in 3–4 wk. These shoots rooted with 100% efficiency on MS basal medium,
and plants developed that showed normal growth and flowering under greenhouse conditions. 相似文献
18.
Plantlet regeneration through shoot formation from young leaf explant-derived callus of Camptotheca acuminata is described. Calli were obtained by placing leaf explants on Woody plant medium (WPM) supplemented with various concentrations
of 6-benzyladenine (BA) and naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). Callus induction was observed
in all media evaluated. On the shoot induction medium, the callus induced on the WPM medium containing 19.8 μM BA and 5.8
μM NAA was the most effective, providing high shoot regeneration frequency (70.3 %) as well as the highest number of shoots
(11.2 shoots explant−1). The good rooting percentage and root quality (98 %, 5.9 roots shoot−1) were achieved on WPM medium supplemented with 9.6 μM indole-3-butyric acid (IBA). 96 % of the in vitro rooted plantlets with well developed shoots and roots survived transfer to soil. 相似文献
19.
An in vitro method for propagation of Holarrhena antidysenterica Wall. has been developed using nodal explants from mature trees growing in the field. Irrespective of concentrations and
combinations of growth regulators used, the axillary and terminal buds sprouted and elongated when inoculated on Murashige
and Skoog (MS) medium. The highest numbers of shoots were formed when sprouted shoots were subcultured from MS basal medium
onto MS medium containing 2 mg dm−3 N6-benzyladenine (BA) and 0.5 mg dm−3 α-naphthalene acetic acid (NAA). The shoot number further increased upon subculture on MS medium containing 0.5 mg dm−3 BA. By repeated sub-culturing of shoots derived from nodal axillary buds, a high frequency multiplication rate was established.
The elongated shoots were excised and rooted in auxin free MS basal medium. Ex vitro rooting of in vitro formed shoots was achieved upon dipping the microshoots for 2 min in 2 mg dm−3 of indole-3-butyric acid solution. Successful field establishment and high (80–90 %) survival of plants was observed. 相似文献
20.
Rakhi Chaturvedi S. P. Bhatnagar 《In vitro cellular & developmental biology. Plant》2001,37(2):255-258
Summary A protocol for in vitro shoot regeneration from cotyledon explants of Citrullus lanatus (Thunb.) Matsum. & Nakai cv. Sugar Baby is described. The cotyledons excised from 7-d-old aseptic seedlings showed the highest
percentage of shoots on Murashige and Skoog (MS) + N6-benzyladenine (BA; 3.0 μM) + N6-[2-isopentenyl] adenine (2iP; 3.0 μM) and MS + BA (3.0 μM) + indole-3-acetic acid (IAA; 3.0 μM). Whereas the latter medium induced shoot regeneration after the callusing of the explant, the former stimulated direct shoot
formation. The regenerated shoots were rooted and the resulting plants were established in earthen pots with 55% success. 相似文献