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1.
In vitro regeneration of evergreen azalea from leaves 总被引:3,自引:0,他引:3
M. Mertens S. Werbrouck G. Samyn H. Botelho dos Santos Moreira da Silva P. Debergh 《Plant Cell, Tissue and Organ Culture》1996,45(3):231-236
Rhododendron simsii Hellmut Vogel was regenerated using different types of explants, auxins and cytokinins. After a callus induction phase, with 2,4-dichlorophenoxyacetic acid or -naphthaleneacetic acid, adventitious shoot regeneration was obtained on a medium supplemented with thidiazuron or zeatin. With thidiazuron shoots were small and a subsequent elongation step was required before rooting. An elongation step was not required when zeatin was used. The duration of the callus induction phase was negatively correlated with the regeneration capacity.Abbreviations 2,4-d
2,4-dichlorophenoxyacetic acid
- NAA
-naphthaleneacetic acid
- 2iP
6-(--dimethylallylamino)purine
- NOA
ß-naphthoxyacetic acid
- BA
6-benzyladenine
- IBA
1-H-indole-3-butyric acid
- IAA
1-H-indole-3-acetic acid
- TDZ
thidiazuron
- WPM
woody plant medium 相似文献
2.
The effects of basal media, growth regulators and gelling agents on the morphogenetic response of eleven Cucumis melo L. var. reticulatus and inodorus genotypes were examined. Regeneration was achieved from cultured cotyledons of all genotypes and the morphogenic response was affected by the genetic background. Among the combination of factors tested, MS basal medium supplemented with 2.8 M 6-benzyladenine (BA) and 1.0 M abscisic acid (ABA) solidified with agar gave the highest frequency of shoot regeneration.Abbreviations ABA
abscisic acid
- BA
6-benzyladenine
- TDZ
thidiazuron 相似文献
3.
Callus induction and plant regeneration from gladiolus 总被引:1,自引:0,他引:1
A method for the initiation of callus capable of plant regeneration from in vivo grown cormels of gladiolus (Gladiolus x grandiflorus Hort.) is described. Sliced cormels of the large-flowering hybrid, Peter Pears were cultured in vitro on a modified Murashige and Skoog medium, supplemented with various auxins. Yellow callus, which was either friable or compact, could be induced on all media tested. Callus induced on media with naphthaleneacetic acid failed to proliferate. Callus induced on media with 9 mM 2,4-dichlorophenoxyacetic acid showed the best growth. Addition of micro-elements and vitamins increased the induction and growth of callus capable of plant regeneration. Explants taken from the middle part of the cormels had the highest competence for callus initiation. Callus was induced on several gladiolus hybrids and the South African species G. garnierii Klatt. Callus induction was genotype dependent and among the cultivars tested, Peter Pears and White Prosperity were superior with respect to callus production on the media with either 2,4-dichlorophenoxyacetic acid or picloram. Plants were regenerated from yellow compact callus of all genotypes on media containing zeatin and benzyladenine in various concentrations.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- MS
Murashige and Skoog basal salt and vitamins (1962)
- CI
callus induction medium
- NAA
-naphthaleneacetic acid
- BA
6-benzyladenine
- picloram
4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid
- zeatin
6-[4-hydroxy-3-methylbut-2-enylamino]purine 相似文献
4.
An efficient in vitro method for mass propagation of a woody ornamentalIxora coccinea L. 总被引:1,自引:0,他引:1
Various factors that affect culture establishment, shoot growth, proliferation and rooting ofIxora coccinea L., a woody shrub, were studied. Stem cuttings (decapitated shoot, three nodes) were the most suitable explants for multiple-shoot proliferation, and when cultured on a woody plant medium (WPM) containing 2.5 M BA produced axillary shoots which branched repeatedly, yielding an average of 27 shoots per explant after 6 weeks in culture. Kinetin, 2-iP, zeatin and thidiazuron all induced multiple-shoot formation, but were less effective than BA. While the presence of IAA in the multiplication medium was detrimental to shoot proliferation, shoot growth was not affected by IAA. The production of large amounts of basal callus and vitrification of shoots were the major problems to be avoided in proliferating shoot cultures. Addition of TIBA to the multiplication medium markedly reduced basal callusing, while sealing the culture vessels with a fluorocarbon polymer (tetrafluoroethyleneperfluoroalkyl vinyl ether) film (Neoflon PFA film) almost completely eliminated vitrification. A reduction in the number of vitrified shoots was also achieved with AVG treatment. Following this protocol of using BA-supplemented WPM and Neoflon film, it would be possible to produce more than 100,000 plants from a single stem cutting in 1 year.Abbreviations
AVG
Aminoethoxyvinylglycine
-
BA
N6-benzyladenine
-
BM
basal medium
-
IAA
indole-3-acetic acid
-
IBA
indole-3-butyric acid
-
2-tiP
N6-(2-isopentenyl)adenine
-
KIN
kinetin
-
MS
Murashige and Skoog medium
-
NAA
-naphthaleneacetic acid
-
SRM
shoot regeneration medium
-
TDZ
thidiazuron
-
TIBA
2,3,5-triiodobenzoic acid
-
WPM
woody plant medium
-
ZEA
zeatin 相似文献
5.
Stimulation of in vitro shoot proliferation from nodal explants of cassava by thidiazuron, benzyladenine and gibberellic acid 总被引:2,自引:0,他引:2
Basdeo Bhagwat Luiz G. F. Vieiral Larry R. Erickson 《Plant Cell, Tissue and Organ Culture》1996,46(1):1-7
Multiple shoots were produced from nodal explants of cassava (Manihot esculenta Crantz) by a two-step procedure: a 6- to 8-day exposure to 0.11–0.22 µM thidiazuron (TDZ) in liquid Murashige and Skoog (MS) medium followed by culture on agar-solidified MS medium supplemented with 2.2 µM 6-benzyladenine (BA) and 1.6 M gibberellic acid (GA3). TDZ caused the nodal explants to expand and this expansion (growth) continued during culture with BA and GA3. From this expanded explant, clusters of buds and fasciated stems developed continuously and these gave rise to shoots. The shoot proliferation process was open-ended, yielding an average of 31.5 shoots per nodal explant after 10 weeks of culture with genotype CG 1–56. A positive response was also obtained from seven other genotypes evaluated with this protocol.Abbreviations BA
6-benzyladenine
- BM
basal medium
- DPU
1,3-diphenylurea
- GA3
gibberellie acid
- 2iP
isopentenyladenine
- MSM
multiple shoot medium
- NAA
1-naphthaleneacetic acid
- PGR
plant growth regulator
- TDZ
thidiazuron
- Z
zeatin 相似文献
6.
Petioles, leaf discs and midribs of several olive (Olea europaea L.) cultivars, collected from potted greenhouse plants, field-grown and in vitro shoots, were used to test their morphogenic capacity. Adventitious shoots were induced only in petioles from in vitro-grown shoots of cultivars Moraiolo, Dolce Agogia and Halkidikis, grown on Olive Medium (OM) plus 18 M zeatin within 4 to 5 weeks. Regeneration was achieved, both on Murashige and Skoog (MS) and on modified OM, only in the dark. The highest regeneration was achieved directly from the proximal part of the petioles after 2 to 3 weeks in media containing 5 to 40 M thidiazuron, or with both 10 M 2-isopentenyladenine +2.2 M 6-benzyladenine with or without low auxin concentration (not more than 2.5 M). A few adventitious shoots were also regenerated from callus when it was shifted from auxin and cytokinin media to cytokinin only medium. The regeneration potential was higher in petioles collected from apical nodes than from basal ones. The adventitious shoots were transferred to solid half-strength MS medium supplemented with 4.5 M zeatin for further development. Several regenerated shoots were rooted and the plantlets hardened in the greenhouse. No apparent differences regarding morphological aspects were observed among the regenerated plantlets or with those obtained by stimulation of axillary buds.Abbreviations BA
6-benzyladenine
- IBA
indole-3-butyric acid
- NAA
1-naphthaleneacetic acid
- TDZ
thidiazuron (N-phenyl-N-1,2,3-thidiazol-5-ylurea)
- 2iP
2-isopentenyladenine
- MS
Murashige and Skoog medium
- 1/2 MS
half strength MS
- OM
Olive Medium
- BN
Bourgin & Nitsch 相似文献
7.
The chinese tree Camptotheca acuminata produces the anti-cancer and anti-retroviral drug camptothecin. Methods were developed for the clonal propagation of this important medicinal plant through shoot bud culture. Shoot buds were excised from 25 to 30 day old seedlings, presoaked for 48 h in three different liquid media containing either BA (2.22–17.4 M), kinetin (2.32–18.58 M), or thidiazuron (0.1–10 M) and were subsequently cultured on semi-solid medium of the same composition. Multiple shoots only developed from the 6-benzyladenine presoaked explants with the maximum number of shoots initiated from buds presoaked in and grown on B5 medium containing 17.4 M 6-benzyladenine. Individual shoots were removed from clusters and rooted on B5 supplemented with indole-3-butyric acid (4.9–19.6 M). The lowest concentration of indole-3-butyric acid (4.9 M) gave the highest percentage of rooting (82%) and the shortest root initiation period (18 d). Over 90% of the in vitro rooted plantlets survived transfer to soil.Abbreviations BA
6-benzyladenine
- B5
Gamborg's B5 medium (Gamborg et al., 1968)
- CPT
camptothecin
- 2,4-d
2,4-dichlorophenoxyacetic acid
- IBA
indole-3-butyric acid
- kinetin
6-furfurylaminopurine
- LS
Linsmaier & Skoog medium (Linsmaier & Skoog, 1965)
- MS
Murashige & Skoog (Murashige & Skoog, 1962)
- NAA
I-naphthaleneacetic acid
- PGR
plant growth regulator
- TDZ
thidiazuron
- WPM
woody plant medium (Lloyd & McCown, 1981) 相似文献
8.
S. P. O. Werbrouck P. Redig H. A. Van Onckelen P. C. Debergh 《Journal of Plant Growth Regulation》1996,15(2):87-93
Imidazole fungicides such as imazalil, prochloraz, and triflurnizole and the triazole growth retardant paclobutrazol promote the shoot-inducing effect of exogenous cytokinins in Araceae, such as Spathiphyllum floribundum Schott and Anthurium andreanum Schott. The mechanism of their action could partially be based on the inhibition of gibberellic acid (GA) biosynthesis, because administration of GA3 inhibits the phenomenon completely in S. floribundum. Not only is the suppression of GA biosynthesis involved, but also the metabolism of endogenous cytokinins is significantly altered. Although the balance between isopentenyladenine, zeatin, dihydrozeatin, and their derivatives was shifted to distinguished directions by administration of BA and/or imazalil and/or GA3, no correlation between these changes in metabolic pathways and the number of shoots could be found. The metabolism of BA was not significantly altered by adding imazalil to the micropropagation medium of S. floribundum.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- [9R-5P]DHZ
9--d-ribofuranosyl-dihydrozeatin-monophosphate
- [9R-5P]iP
6-isopentenyl-9--d-ribofuranosyladenine-monophosphate
- [9R-5P]Z
9--d-ribofuranosyl-zeatin-monophosphate
- [9G]BA
6-benzyl-9--d-glucopyranosyladenine
- [9G]DHZ
9--d-glucopyranosyl-dihydrozeatin
- [9G]iP
6-isopentenyl-9--d-glucopyranosyladenine
- [9G]Z
9--d-glucopyranosyl-zeatin
- [9R]BA
6-benzyl-9--d-ribofuranosyladenine
- [9R]DHZ
9--d-ribofuranosyl-dihydrozeatin
- [9R]iP
6-isopentenyl-9--d-ribofuranosyladenine
- [9R]Z
9--d-ribofuranosyl-zeatin
- BA
6-benzyladenine
- DHZ
dihydrozeatin
- ES+ LC-MS/MS
HPLC coupled Electrospray Tandem Mass Spectrometry
- f.m.
fresh mass
- mT
6-(3-hydroxybenzyl)adenine
- IMA
imazalil
- iP
isopentenyladenine
- NAA
1-naphthalene acetic acid
- NFT
Nutrient Film Technique
- (OG)[9R]DHZ
O--glucopyranosyl-9--d-ribofuranosyl-dihydrozeatin
- (OG)[9R]Z
O--d-glucopyranosyl-9--d-ribofuranosyl-zeatin
- (OG)DHZ
O--d-glucopyranosyl-dihydrozeatin
- (OG)Z
O--d-glucopyranosyl-zeatin
- PAR
Photosynthetic Active Radiation
- PBZ
paclobutrazol
- PRO
prochloraz
- TDZ
thidiazuron
- TRI
triflurnizole
- Z
zeatin 相似文献
9.
For the purpose of developing an in vitro regeneration system for chickpea (Cicer arietinum L.), an important food legume, immature cotyledons approximately 5 mm long were excised from developing embryos and cultured on B5 basal medium supplemented with 1.5% sucrose and various growth regulator combinations. Only non-morphogenic callus was formed in response to concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) previously reported to induce somatic embryogenesis on immature soybean cotyledons. However, 4.6, 13.7, and 45.6 M zeatin induced formation of white, cotyledon-like structures (CLS) at the proximal end of immature cotyledons placed with adaxial surface facing the agar medium. No morphogenesis, or occasional formation of fused, deformed CLS, was observed when zeatin was replaced with kinetin or 6-benzyladenine, respectively. The highest response frequency, 64% of explants forming CLS, was induced by 13.7 M zeatin plus 0.2 M indole-acetic acid (IAA). Within 20–40 days culture on zeatin, shoots formed at the base of CLS on approximately 50% of CLS-bearing explants, and proliferated upon subsequent transfer to basal medium with 4.4 M BA or 4.6 M kinetin. This regeneration system may be useful for genetic transformation of chickpea. 相似文献
10.
Adventitious shoot regeneration was compared among leaf, stem and petal explants of carnation (Dianthus caryophyllus L.) cv. Scania on MS medium containing different concentrations of 6-benzyladenine (BA) and -naphthaleneacetic acid (NAA). High frequency regeneration was obtained only from petal explants on the media containing 5 to 10 M BA with or without 5 M NAA. Among the cytokinins tested, N-2-chloro-4-pyridyl-N-phenylurea and N-1,2,3-thiadiazol-5-yl-N-N-phenylurea were more effective than BA, kinetin, N6-2-isopentenyl adenine and zeatin on regeneration from petal explants. Although, high frequency shoot regeneration was obtained from all petal explants harvested from various developmental stages of buds, a significant decrease in regeneration capacity was observed in the explants obtained from fully-opened flowers. High frequency shoot regeneration was also obtained from the petal explants of cvs. Coral. Lena, Nora and White Sim, and an interspecific cultivar Eolo using the method developed in this study.Abbreviations NAA
-naphthaleneacetic acid
- BA
6-benzyladenine
- GA3
gibberellic acid
- 2iP
N6-2-isopentenyl adenine
- KT-30
N-2-chloro-4-pyridyl-N-phenylurea (also called 4PU)
- TDZ
N-1,2,3-thiadiazol-5-yl-N-phenylurea (also called thidiazuron) 相似文献
11.
Massimo H. M. Sanago Vern I. Shattuck Judith Strommer 《Plant Cell, Tissue and Organ Culture》1996,45(2):165-168
A simple and rapid pea regeneration procedure was developed. An average of up to 20 shoots formed from hypocotyl explants of cvs. Sugar Ann and Patriot cultured on Murashige and Skoog basal medium supplemented with 0.5 or 1.0 M thidiazuron (N-phenyl-N-1,2,3-thiadiazol-5-ylurea). Hypocotyls of Puget and Sugar Daddy did not respond. Regenerated shoots rooted rapidly when cultured on Murashige and Skoog basal medium containing either 2.0 M -naphthaleneacetic acid or 1.0–2.0 M indole-3-butyric acid. Seeds were harvested from regenerated plants after only 9–11 weeks.Abbreviations BAP
6-benzyladenine
- 2,4-d
2,4-dichlorophenoxy acetic acid
- GA3
gibberellic acid
- IBA
indole-3-butyric acid
- MS
Murashige and Skoog (1962) medium
- NAA
-naphthaleneacetic acid
- TDZ
thidiazuron (N-phenyl-N-1,2,3-thiadiazol-5-ylurea) 相似文献
12.
Agrobacterium-mediated transformation of hybrid poplar suspension cultures and regeneration of transformed plants 总被引:6,自引:0,他引:6
Glenn T. Howe Barry Goldfarb Steven H. Strauss 《Plant Cell, Tissue and Organ Culture》1994,36(1):59-71
A method for Agrobacterium-mediated transformation of hybrid poplar (Populus alba x P. grandidentata cv. Crandon) suspension cultures and regeneration of transformed plants is described. Transformants were recovered when suspension cultures were inoculated with Agrobacterium tumefaciens at a density of 107 colony-forming units ml-1, cocultivated for 48 h, and plated to cellulose acetate filters on Woody Plant Medium containing 4.5 M 2,4-dichlorophenoxyacetic acid and 250 mg l-1 cefotaxime. Levels of cefotaxime greater than 250 mg l-1 were unnecessary for control of residual bacteria and inhibited callus growth. Transgenic plants were regenerated by culturing the transformed callus on media containing 0.11 to 27 M thidiazuron. In contrast to thidiazuron, N6-benzyladenine had a negative effect on shoot regeneration; the callus became necrotic when we attempted to induce shoots with concentrations of 1.1 to 8.9 M, and growth was inhibited when concentrations of 0.11 or 0.22 M were used to regenerate callus from suspension cultures. Following cocultivation of poplar suspension cultures, we recovered transgenic plants containing the maize transposon Ac, and callus containing an insect toxin gene from Bacillus thuringiensis.Abbreviations BA
N6-benzyladenine
- CIM
callus initiation medium
- CaMV
cauliflower mosaic virus
- cfu's
colony-forming units
- HPT
hygromycin phosphotransferase
- MS
Murashige and Skoog medium (Murashige & Skoog 1962)
- NPT-II
neomycin phosphotransferase-II
- PAR
photosynthetically active radiation
- PCR
polymerase-chain-reaction
- TDZ
thidiazuron
- WPM
Woody Plant Medium (Lloyd & McCown 1980)
- 2,4-d
2,4-dichlorophenoxyacetic acid 相似文献
13.
Meconopsis simplicifolia (D.Don) Walp. could be propagated by induction of adventitious shoots from callus produced on hypocotyl, cotyledon and rosette leaf explants of 4-month-old seedlings. Callus was initiated on agar-solidified Murashige and Skoog medium supplemented with 1 M kinetin +10 M -naphthaleneacetic acid (NAA). Shoots formed when the callus was subcultured on medium supplemented with kinetin or benzyladenine (BA) in combination with NAA, indoleacetic acid, indolebutyric acid or gibberellic acid. Excised shoots were rooted on medium containing auxin with 10 M NAA producing the best rooting (55%).Abbreviations BA
6-benzyladenine
- 2,4-d
2,4-dichlorophenoxy-acetic acid
- FAA
formalin-acetic acid-alcohol
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- IBA
-indolebutyric acid
- NAA
-naphthaleneacetic acid 相似文献
14.
An in vitro propagation protocol based on axillary bud proliferation has been developed for mature female trees of Ceratonia siliqua L. `Galhosa' and `Mulata'. Browning and contaminants were the major obstacles for culture establishment. Shoot culture initiation was greatly influenced by explanting season, with the highest survival percentage observed in spring. The cultivar, cytokinin type and concentration were the most important factors affecting shoot multiplication. The best multiple-shoot response was obtained with `Mulata' on Murashige and Skoog medium supplemented with 4.44 M 6-benzyladenine or 4.56 M zeatin. Rooting was achieved on growth-regulator-free medium after basal dipping of shoots in indole-3-butyric acid (4.9 mM). Plantlets were successfully acclimatized (80–85%) under high relative humidity and then moved to the glasshouse. A field trial was established to follow their agronomic behaviour. 相似文献
15.
Floral induction and development in Phalaenopsis in vitro 总被引:10,自引:0,他引:10
Adventitious shoots formed from nodal sections of floral-stalks of Phalaenopsis Pink Leopard Petra that had been propagated on Hyponex medium with 6-benzyladenine (BA). After subculturing adventitious shoots on Vacin-Went medium with BA, more than 70% of the shoots formed floral buds, and 10% of the floral buds flowered in 9-month-old shoots. Floral buds were not formed on the adventitious shoots cultured on Vacin-Went medium lacking BA. A high concentration of total nitrogen inhibited the development of floral buds, and low-temperature treatments failed to induce the formation of floral buds in vitro. 相似文献
16.
Paclitaxel production in suspension cell cultures of Taxus 总被引:3,自引:0,他引:3
Five separate cell lines, three of Taxus canadensis Marsh. and two of Taxus cuspidata Sieb. et Zucc., were used to test the effect of carbohydrates and plant growth regulators on the growth of cells and production of paclitaxel in culture. There was no significant correlation between growth of cells and paclitaxel production. While no single medium was developed that was optimal for all cell lines, it was possible to develop a medium for each species that represented a superior combination of growth and paclitaxel production. A combination of NAA and thidiazuron produced the best combination of growth and paclitaxel production in cell lines of T. canadensis, while IAA and BA produced the best results in cell lines of T. cuspidata. A mixture of sucrose and fructose gave the best combination of growth and paclitaxel production. The addition of carbohydrates midway through the growth cycle increased the rate at which paclitaxel accumulated in the culture medium. The highest paclitaxel concentration obtained was 14.78±0.86 mg 1–1 (n=3).Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- 2ip
6-(,-dimethylamino)-purine
- BA
6-benzyladenine
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- kinetin
6-furfurylaminopurine
- NAA
-napthaleneacetic acid
- picloram
4-amino-3,5,6-trichloropicolinic acid
- thidiazuron
1-phenyl-3 (1,2,3-thiadiazol-5-yl)urea 相似文献
17.
In this work, a combined HPLC-ELISA technique was used to associate in vitro rooting capacity of tree peony micro-cuttings with contents of cytokinin and auxin; the cytokinin mainly detected corresponded to the N6-benzyladenine which had been added to the multiplication medium. Rooting capacity of explants was favoured by a preliminary accumulation of endogenous IAA only when levels of the BA absorbed from the multiplication medium had decreased. Main shoots coming from a 5-weeks subculture fulfilled these hormonal conditions and were the best microcuttings for rooting (87% rooting). Main shoots coming from shorter cycles or axillary shoots coming from a 5-weeks cycle always contained high benzyladenine levels and had a low rooting capacity (25–55% rooting). Root induction was associated with an early peak of indole-3-acetic acid followed by a 10-fold lower peak of endogenous ribofuranosyl-isopentenyladenine. Only a low and transitory accumulation of isopentenyladenine occurred during root development, and this could explain the lack of shoot development. Root development was efficient, especially in a medium containing activated charcoal, which led to an almost 3-fold decrease of IAA contents in roots.Abbreviations AC
activated charcoal
- BA
N6-benzyladenine
- ELISA
enzyme linked immunosorbent assay
- HPLC
high performance liquid chromatography
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- iP
N6-(2-isopentenyl)adenine
- RDM
root development medium
- RIM
root induction medium
- 9RIP
9--d-ribofuranosyl-iP
- 9RZ
9--d ribofuranosyl-zeatin
- Z
zeatin 相似文献
18.
The effect of zeatin, NAA (-naphthaleneacetic acid), putrescine and cefotaxime on the frequency of shoot regeneration from Betula pendula Dalecarlica EM 85 leaf discs has been examined. About 80% of leaf discs were induced to form adventitious shoots when the culture medium contained 45.6 moll-1 zeatin and 0.1 mmoll-1 cefotaxime. The addition of NAA to zeatin-containing media prevented shoot regeneration but stimulated root development directly from leaf tissues. Putrescine (0.1 mmoll-1) and cefotaxime (0.1 mmoll-1) could both significantly increase the percentage of leaf discs regenerating on optimal zeatin-containing media, and increase the number of shoots per regenerating disc.Abbreviations NAA
-naphthaleneacetic acid
- BAP
6-benzyladenine 相似文献
19.
An efficient and reproducible procedure is described for the large-scale propagation of an epiphytic orchid,Acampe praemorsa (Roxb.) B latter and McCann using foliar explants. Shoot buds were induced in basal parts of foliar explants on Murashige and Skoog medium supplemented with N6-benzyladenine (BA), kinetin (Kn) or thidiazuron (TDZ), the latter being most effective at 1.0 mg/1. Shoots formed to a TDZ-containing medium elongated following transfer to a substrate supplemented with 2.0 mg/l 1-naphthaleneacetic acid (NAA) and 0.5 mg/1 BA. NAA at lower concentrations had no beneficial effects on shoot regeneration, whether added to the medium along with BA, Kn or TDZ. However, it promoted shoot elongation and leaf expansion. Higher concentrations of NAA suppressed shoot regeneration. The frequency of shoot regeneration was greatly influenced by the developmental stage and orientation of the leaf. Shoots regenerated from the foliar explants were rooted successfully on MS medium containing 1.0 mg/l indole-3-butyric acid. The plantlets were acclimated and eventually transferred to a garden.Abbreviations
BA
N6-Benzyladenine
-
IAA
Indole-3-acetic acid
-
IBA
Indole-3-butyric acid
-
Kn
Kinetin
-
MS
Murashige and Skoog (1962) medium
-
NAA
1-Naphthaleneacetic acid
-
TDZ
Thidiazuron (N-phenyl-N-1,2,3-thiadiazol-5-ylurea) 相似文献
20.
Bernard Malaurie Jean-Claude Thouvenel Okito Pungu 《Plant Cell, Tissue and Organ Culture》1995,42(2):215-218
In vitro morphological development of meristem-tips, excised from microshoots, is reported for one clone Grosse Caille of D. cayenensis-D. rotundata complex and for one clone of D. praehensilis. The effects of size and location of meristem-tip on viability and morphological development after 3, 8 and 11 months in vitro culture were studied. The larger size of meristem-tip gave the best rooting and plantlet formation while the smaller size gave the best bud production. Axillary buds were more reactive than apical meristem-tips in callusing and rooting when small-sized meristems of the Grosse Caille clone were used; the use of larger meristem-tips did not induce more differences between apical and axillary buds. The production of plantlets was successful, after 11 months of culture, with a score of 82 and 39 % survivors, for Grosse Caille clone and D. praehensilis genotype, respectively.Abbreviations Ap
apical meristem-tips
- ApLS
large-sized apical meristem-tips
- Ax
axillary meristem-tips
- AxLS
large-sized axillary meristem-tips
- BA
6-benzyladenine
- cv
cultivar
- LS
large-sized meristem-tip
- NAA
naphthalenacetic acid
- SS
small-sized meristem-tip 相似文献