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1.
Ash and silica content and their depositional patterns in differenttissues of the mature corn plant (Zea mays L.) were determined.Ash and silica were highest in the leaf blades (up to 16.6 and10.9 per cent, respectively) followed by the leaf sheath, tassel,roots, stem epidermis and pith, and ear husk. The percentageof ash as silica was also highest in the leaves. Silica wasextremely low in the kernels. The upper stem epidermis and pithcontained nearly twice the silica content as did the lower portion.The patterns of ash and silica distribution were similar inplants grown in two different areas of Kansas, but were in lowerconcentration in the leaves and leaf sheaths from the area withlower soluble silica in the soil. Silica was deposited in theepidermis in a continuous matrix with cell walls showing serratedinterlocking margins in both leaves and stem. Rows of lobedphytoliths of denser silica were found in the epidermis as wellas highly silicified guard cells and trichomes. The silica matrixof the epidermis appears smooth on the outer surface and porousor spongy on the inner surface. Zea mays L. Corn, maize, ash content, silica deposition, scanning electron microscopy 相似文献
2.
Identification of maize silicon influx transporters 总被引:1,自引:1,他引:0
Maize (Zea mays L.) shows a high accumulation of silicon (Si),but transporters involved in the uptake and distribution havenot been identified. In the present study, we isolated two genes(ZmLsi1 and ZmLsi6), which are homologous to rice influx Sitransporter OsLsi1. Heterologous expression in Xenopus laevisoocytes showed that both ZmLsi1 and ZmLsi6 are permeable tosilicic acid. ZmLsi1 was mainly expressed in the roots. By contrast,ZmLsi6 was expressed more in the leaf sheaths and blades. Differentfrom OsLsi1, the expression level of both ZmLsi1 and ZmLsi6was unaffected by Si supply. Immunostaining showed that ZmLsi1was localized on the plasma membrane of the distal side of rootepidermal and hypodermal cells in the seminal and crown roots,and also in cortex cells in lateral roots. In the shoots, ZmLsi6was found in the xylem parenchyma cells that are adjacent tothe vessels in both leaf sheaths and leaf blades. ZmLsi6 inthe leaf sheaths and blades also exhibited polar localizationon the side facing towards the vessel. Taken together, it canbe concluded that ZmLsi1 is an influx transporter of Si, whichis responsible for the transport of Si from the external solutionto the root cells and that ZmLsi6 mainly functions as a Si transporterfor xylem unloading. 相似文献
3.
Alexander Lux Miroslava Luxová Jun Abe Shigenori Morita Shinobu Inanaga 《Plant and Soil》2003,255(1):85-91
Bamboo is a silicon accumulating plant. In leaves, the major place of silicon (Si) deposition is the epidermis, with the highest concentration of Si in silica cells. In bamboo roots, the deposition of Si is found only in endodermal cell walls. The silicification of leaves and roots was examined in the economically important bamboo plant Phyllostachys heterocycla, using an environmental scanning electron microscope coupled with X-ray microanalysis, as well as gravimetric quantification. The content of Si on a dry weight basis measured by gravimetric quantification was 7.6% in leaves and 2.4% in roots, respectively. Moreover, quantification of EDX data showed high Si impregnation of the inner tangential endodermal walls. Si content in this part of the root endodermal cell walls was even higher than that in the outer leaf epidermal walls, where conspicuous deposition of Si often occurs in grass plants. 相似文献
4.
The Effects of Silicon Supplementation on Cucumber Fruit: Changes in Surface Characteristics 总被引:3,自引:0,他引:3
Addition of silicate to hydroponic nutrient media has been shownto increase resistance of cucumber to powdery mildew. As a sideeffect of this treatment, the fruit surface developed an unusualdull appearance, or bloom. The effects of silicate supplementationon cucumber fruit were observed using scanning and transmissionelectron microscopy, energy-dispersive X-ray analysis, and colourimetricassays. Changes in the fruit trichome morphology occurred: trichomesfrom + Si fruit had a coarse outer appearance compared to -Si fruit where the trichomes were smooth. X-ray mapping showedhigh Si content in trichomes but not in the surrounding epidermisor fleshy mesocarp and endocarp tissues. Sections through theepidermal layer reveal that the silica is restricted to thetrichomes, primarily in the epicuticular wax.Copyright 1993,1999 Academic Press Cucumber, Cucumis sativus L., fruit, silica, energy dispersive X-ray analysis, trichomes 相似文献
5.
A. G. Sangster 《American journal of botany》1978,65(9):929-935
Silicon (Si) distribution in the roots of Sorghastrum nutans (L.) Nash and Sorghum bicolor (L.) Moench. was investigated by means of the electron-probe microanalyzer and scanning electron microscope. In both species, Si was confined to the inner tangential wall of the tertiary-phase endodermal cells in the form of nodular silica aggregates of similar morphology and X-ray intensity. The results are compared to those for six closely related genera, as well as to studies of Si in the roots of species of other tribes of the family Poaceae. The various types of root deposits occurring in the family are described, and their relationships discussed. It is concluded that the type of Si distribution exhibited is determined largely by the phylogenetic status of the genus, rather than by the basic pattern of root anatomy. 相似文献
6.
Silica and Ash in Seeds of Cultivated Grains and Native Plants 总被引:1,自引:0,他引:1
Silica and ash contents and the depositional patterns of opalinesilica have been determined in the seeds of 31 plant species.Included were 13 monocotyledons, eight dicotyledons and theseeds of eight common cereal grains. The cereal grains, exceptfor Oryza sativa L. (3.2%) and Avena sativa L. (1.4%), werequite low in silica. The silica in these cereals was in thelemma. In seeds with high silica content it often makes up morethan 50% of the ash. Silica in seeds occurs largely in the outercoating of the seed. Dicotyledon seeds tend to have less silicathan those of monocotyledons. Energy-dispersive X-ray analysisshows that the distribution of the element silicon is clearlyrelated to certain epidermal structures such as ridges, raisedareas, trichomes and hairs. It also occurs in cell walls. Membersof a specific plant family tend to have very similar silicadepositional patterns in their seeds. Small amounts of K, S,Cl and Ca are also found in seeds. Light-microscopy studiesshow that the silica in the lemma of seeds such as Oryza sativaL. is deposited in cellular sheet-like structures with crenateedges. Silica in seeds also occurs in fibres and in other cellularstructures (silica cells) that become phytoliths. Seeds, epidermis, seed coat, silica and ash content, scanning electron microscopy, energy-dispersive X-ray analysis, silica depositional patterns, trichomes 相似文献
7.
Early silicification of leaves and roots of seedlings of a panicoid grass grown under different conditions: anatomical relations and structural role
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M. Fernández Honaine M. L. Benvenuto N. L. Borrelli M. Osterrieth 《Plant biology (Stuttgart, Germany)》2016,18(6):1025-1030
- Grasses accumulate high amounts of silica deposits in tissues of all their organs, especially at mature stage. However, when and under which conditions do grass seedlings begin to produce these silica deposits and their relation with anatomy and development is little known. Here we investigated the silicification process in the first leaves and roots of seedlings of Bothriochloa laguroides grown in different substrate and Si treatments.
- The distribution and content of silica deposits in the organs of the seedlings grown under different conditions were analyzed through staining techniques and SEM‐EDAX analyses.
- Leaf silica deposits were accumulated 3–4 days after the first leaf emergence, also under low silica solution (0.17–0.2 mM). Their location was mainly restricted to short costal cells from basal sectors, and scarcely in trichomes and xylem at tips. Silica content in leaves increased with the age of the seedlings. Roots presented dome‐shaped silica aggregates, between 4–12 μm of diameter, located in the inner tangential wall of endodermal cells and similar to those produced at maturity.
- Silicification begins early in the first photosynthetic leaf, and silica distribution is opposite to that found in mature plants, mainly restricted to basal sectors, probably acting as a reinforcing element. The fast incorporation of solid amorphous silica in leaves and roots, may be useful for farm applications in species that are Si‐fertilized.
8.
Silica Deposition in Some C3 and C4 Species of Grasses, Sedges and Composites in the USA 总被引:2,自引:0,他引:2
We report new information on silica deposition in 15 plant species,including nine grasses, two sedges and four composites. Thesilica depositional patterns found in seven of the grass speciesindicate that they are C4 plants. However the festucoid grassCortaderia selloana is a C3 plant with long leaf trichomes andoval silica structures in the leaves. In contrast the panicoidC4 grasses Chasmathium latifolium, Chasmathium sessiflorum,Imperata cylindrica, Panicum repens, Panicum commutatum andSetaria magna, all produce dumb-bell-shaped silica structuresin the leaves. The chloridoid grasses Spartina patens and Spartinacynosuroides have saddle-shaped structures and no dumb-bellor oval shaped ones. The sedges Rhynchospora plumosa and Scirpuscyperinus were found to have oval phytoliths and may be C3 plants.Our examination of these and other grasses strongly suggeststhat C4 grasses tend to produce the same type of silica cells.Grasses and sedges with C3 type photosynthesis tend to produceoval silica structures. The composite Grindelia squarrosa andsunflowers Helianthus angustifolia, Helianthus atrorubens andHelianthus tuberosus absorb relatively small amounts of siliconand larger amounts of calcium, where both elements deposit inleaf trichomes. We found no clear indicator for the C3 sunflowersor C4 types in the Asteraceae. Helianthus tuberosus leaves havemany trichomes on the adaxial surface. These trichomes havea higher concentration of silica than the surrounding leaf surface.Helianthus tuberosus leaves had much higher ash and silica contentsthan those of Helianthus angustifolia and Helianthus atrorubens.The composite Grindelia squarrosa has a usual deposition ofsilica in the basal cells around the guard cells. Silica depositionoften reflects the surface features of a leaf. An exceptionis Scripus cyperinus where the silica structures are deep inthe tissue and do not reflect the surface configurations. Theinforescence of Setaria magna had a 14.64 silica content. Thetufts of white, silky hairs characteristic of Imperata cylindricainflorescence have no silica. C3 and C4 plants, silica and ash content, scanning electron microscopy, energy-dispersive X-ray analysis, silicon distribution, spectra of elements in plants, trichomes, silica fibres, phytoliths 相似文献
9.
Ash and silica contents and their depositional patterns in differenttissues of 44 Mississippi coastal plants were determined. Silicacontent of dried plants varied from no more than a trace inChenopodium album L. leaves to 7.37 per cent in Zizanopsis miliacea(Michx) Doell & Aschers leaves. Ash content varied from2.50 per cent in Lythrum lineare L. stems to 28.24 per centin Borrichia frutescens (L.) DC leaves. Plants in the same familytend to be alike in their ability to absorb or not absorb silica.Poaceae and Cyperaceae had consistently high concentrationsof silica. In contrast, the Asteraceae studied had very lowsilica contents but often had high contents of other minerals.Dicotyledonous plants studied had consistently lower silicacontents than the monocotyledons. Plants growing in salt watercontained considerable sodium chloride. Spectra were obtainedfor major elements in four different plants. Energy-dispersiveX-ray analysis shows that distribution of the element siliconis clearly related to certain epidermal structures such as guardcells, ridges, dumb-bells and balls that appear in electronmicrographs. Silica was deposited differently in each type ofplant studied. In many of the plants silica was deposited inrows of irregular-shaped particles running lengthwise of theleaf and in guard cells. In others, like Zizanopsis miliacea(Michx) Doell & Aschers, the deposit was sheet-like. Zizaniaaquatica L. not only had a sheet-like deposit, but the depositwas ridged and there were rows of dumb-bell-shaped silica cells.Related plants had similar structures. Euchlaena mexicana Schrad.,Tripsacum dactyloides (L.) L and Manisuris rugosa (Nutt.) Kuntzeall had irregular phytoliths similar to those in Zea mays L. coastal plants, marsh plants, ash content, silica deposition, scanning electron microscopy, energy-dispersive X-ray analysis, silicon distribution, X-ray diffraction patterns, spectra of elements in plants 相似文献
10.
Tamara I. Balakhnina Vladimir V. Matichenkov Teresa Wlodarczyk Aneta Borkowska Magdalena Nosalewicz Irina R. Fomina 《Plant Growth Regulation》2012,67(1):35-43
Barley (Hordeum vulgare L.) was grown in pots with brown loess soil and highly soluble amorphous silicon dioxide as the source of monosilicic acid
to examine its influence on plant growth and adaptive potential under optimal soil watering and flooding. The adaptive potential
of plants was estimated by the concentration of the thiobarbituric acid reactive substances (TBARs) as well as superoxide
dismutase (SOD), guaiacol peroxidase (GPX) and ascorbate peroxidase (AsP) activities. Application of amorphous silica to the
soil increased the Si content in barley shoots and roots and stimulated their growth and biomass production under optimal
soil watering. Soil flooding suppressed the growth both of the (−Si)- and (+Si)-plants. The intensity of oxidative destruction
estimated by the concentration of TBARs was lower in the roots and leaves of the (+Si)-plants. Soil flooding induced SOD activity
in the roots and in the leaves of the (−Si;+flooding) and (+Si;+flooding)-plants, but no significant differences were observed
due to the Si treatment. GPX activity in the roots of (+Si)-plants was higher than in the (−Si)-ones under optimal soil watering,
but under soil flooding no differences between (+Si)- and (−Si)-treatments were observed. AsP activity was not influenced
by Si treatment neither under optimal soil watering nor under flooding. Thus, application of Si stimulates growth processes
of barley shoots and roots under optimal soil watering and decreases intensity of oxidative destruction under soil flooding
without significant changes in the activities of antioxidant enzymes. 相似文献
11.
Rhizome anatomy is described for Miscanthus sacchariflorus (Maxim.)Hackel. Solid silica deposits, detected as elemental siliconby electron-probe microanalysis and energy-dispersive X-rayanalysis, are confined to cell walls of three concentric zonesconsisting of the uniseriate epidermis, and parenchyma layersaround the cortical air lacunae, and the central cavity, respectively.Si is localized in outer tangential walls of the epidermis,while occurring in all walls of nucleated, parenchyma cellsforming the two internal zones. In comparison, the root exhibitsonly one Si zone. Rhizome Si distribution more closely resemblesthat for Phragmites australis, than for related members of theAndropogoneae. P. australis similarly exhibits aerenchyma anda central cavity. Thus, internal anatomy may strongly influencesilicon distribution. A comparison of taxa of four tribes indicatesthat epidermal wall deposition is common, followed by specificinternal localization in up to three zones of perivascular tissues. Silicon accumulation occurs early in the epidermis of the youngapex of M. sacchariflorus, decreasing sharply across an internodetransection. In comparison, the oldest, basal internodes exhibitvery high Si X-ray counts in each of the three zones, the highestoccurring in the most internal zone around the central cavity.Early Si mobilization in the rhizome apex may resist shearingand abrasion during horizontal growth extension, while depositsbordering aerenchyma of older internodes may resist compression. Miscanthus sacchariflorus (Maxim.) Hackel, plume grass, rhizome, silicification, anatomy, aerenchyma 相似文献
12.
Silica and Ash in Native Plants of the Central and Southeastern Regions of the United States 总被引:1,自引:0,他引:1
Ash and silica contents and depositional patterns were determinedfor different tissues of 11 plants growing in the southeasternand central parts of the USA. Silica content was high in theleaves, sheaths and inflorescences of the grasses studied, especiallyso in the inflorescence of the C3 grass, Stipa comata Trise.and Rupr. The ash content was especially high in leaves of Polymniauvedalia L., which are also high in calcium. Calcium depositionwas largely in trichomes and in veins of the leaf. Energy-dispersiveX-ray analysis showed that the distribution of the element siliconis closely related to certain epidermal structures such as ridges,cell walls, rows of irregularly-shaped structures lying lenghthwisealong the leaf, dumb-bell shaped structures and trichomes. Thesestructures also correspond to the phytoliths left behind afterdecay of the plant. The C3 grasses differed from the C4 in thatthey showed oval structures and produced correspondingly ovalphytoliths. Silicified trichomes (particularly in the C3 grasses)and long, narrow, silica fibres were common in the inflorescencesof the grasses studied. These sharp particles could be irritatingto oesophageal and other tissues. Similar fibres in other grasseshave been implicated in certain cancers. High silicificationof the inflorescence structures might afford protection forthe seed, as reported for other grasses. C3 and C4 grasses, silica and ash content, scanning electron microscopy, energy-dispersive X-ray analysis, silicon distribution, spectra of elements in plants, trichomes, silica fibres, phytoliths 相似文献
13.
Seedlings of Sorghum bicolor (L.) Moench. were cultured in nutrientsolution containing 100 p.p.m. SiO2. Over periods up to 14 days,the progressive accumulation of Si deposits was recorded bymeans of the electron-probe microanalyser and the scanning electronmicroscope along the seminal root length. An acropetal, linear gradient of Si deposits developed in theendodermis, beginning at the proximal end of the root after1 day, and subsequently extended distally until after 7 days,the total silicified endodermal zone occupied the proximal 75per cent of the root length, a value which remained relativelyconstant thereafter, in spite of root extension. No Si was detectedbelow this zone towards the apex. This result is believed tobe related directly to the asynchronous gradient of cell maturationexhibited by the endodermis behind the apex, and specificallyto the degree of wall development therein. Opaline silica was deposited only in the endodermis, initiallyon the inner tangential wall (ITW) surface after only 1 day,as spherical masses of coalesced, primary particles for whichthe term silica aggregate is proposed. A thinlayer of silica over the wall surface was formed as a secondaryphase. The aggregates reached mature size after approximately7 days. Conditions favourable to the inception of silica depositionare discussed including the significance of the chemical compositionof the aggregates, and the importance of the degree of cellulosicthickening, as well as the surface characteristics, of the ITW. 相似文献
14.
Silicon deposition in the roots, culm and leaf of canary grass(Phalaris canariensis L.) was investigated using light microscopy,scanning electron microscopy and electron probe microanalysis. In adventitious roots grown in solution silicon was concentratedin four endodermal walls. Silicon was not detected in the endodermisof aerial adventitious roots, but was present in the epidermisand outer cortical cell layers. Silicon deposition in the culm mainly took place in the epidermis,and particularly in epidermal papillae. The silica deposition pattern in the leaf was typical of thesub-group Festucoideae. The leaf blade showed deposits in costalprickle hairs and wavy rods, but few intercostal deposits. Inthe ligule deposition was confined to isolated groups of pricklehairs on the abaxial surface. The major sites of silica depositionin the leaf sheath were the stomatal subsidiary cells, papillaeand intercostal idioblasts. Prickle hairs were much less commonin the sheath than the blade, and costal wavy rods appearedto be absent in the sheath. Phalaris canariensis L., canary grass, silicification, root, culm, leaf, electron probe microanalysis 相似文献
15.
Silicification in sorghum (Sorghum bicolor) cultivars with different drought tolerance 总被引:6,自引:0,他引:6
Sorghum belongs to a group of economically important, silicon accumulating plants. X-ray microanalysis coupled with environmental scanning electron microscopy (ESEM) of fresh root endodermal and leaf epidermal samples confirms histological and cultivar specificity of silicification. In sorghum roots, silicon is accumulated mostly in endodermal cells. Specialized silica aggregates are formed predominantly in a single row in the form of wall outgrowths on the inner tangential endodermal walls. The density of silica aggregates per square mm of inner tangential endodermal cell wall is around 2700 and there is no significant difference in the cultivars with different content of silicon in roots. In the leaf epidermis, silicon deposits were present in the outer walls of all cells, with the highest concentration in specialized idioblasts termed 'silica cells'. These cells are dumb-bell shaped in sorghum. In both the root endodermis and leaf epidermis, silicification was higher in a drought tolerant cultivar Gadambalia compared with drought sensitive cultivar Tabat. Silicon content per dry mass was higher in leaves than in roots in both cultivars. The values for cv. Gadambalia in roots and leaves are 3.5 and 4.1% Si, respectively, and for cv. Tabat 2.2 and 3.3%. However, based on X-ray microanalysis the amount of Si deposited in endodermal cell walls in drought tolerant cultivar (unlike the drought susceptible cultivar) is higher than that deposited in the leaf epidermis. The high root endodermal silicification might be related to a higher drought resistance. 相似文献
16.
Changes in the Root System of Wheat Seedlings Following Root Anaerobiosis: III. Oxygen Concentration in the Roots 总被引:1,自引:0,他引:1
The oxygen status in roots of wheat seedlings (Triticum aestivum)was determined by a volumetric micro-absorption method. Plantsgrew in nutrient solution (aerated or nitrogen-flushed) or onflooded sand up to the 10th day. The roots were then exposedto aerated or hypoxic conditions for several hours before gaswas extracted by reducing the pressure within a concentratedsalt solution or by physical crushing. The oxygen content ofthe extracted gas bubbles was measured with pyrogallol. Comparativeexperiments with the helophytes Phalaris arundinacea and Carexacutiformis yielded similar oxygen concentrations to those alreadydescribed in literature. The concentrations of oxygen (1316%)in young wheat roots were surprisingly high when exposed tonutrient solution flushed with nitrogen gas. Removal of the shoots decreased the oxygen concentration inthe roots, indicating some internal oxygen transport from shootsto roots. Detached, submerged roots of wheat still contained6% oxygen following 20 h of submergence in nitrogen-flushedsolution. A linear relationship was found between the oxygenconcentration in roots of Triticum aestivum, Zea mays and thetwo helophytes and the volume of extractable gas per volumeof root. This ratio corresponded to the extent of aerenchymaformation. Hence, a certain amount of oxygen may have been adsorbedonto the inner surfaces of the lacunae of the roots. However, the large amount of oxygen in the roots of intact wheatplants suggest that some parts of the root system are unlikelyto suffer from the oxygen shortage imposed by oxygen-deficientexternal conditions such as flooded soil. Triticum aestivum L. cv. Hatri, wheat, helophytes, roots, micro-absorption method, oxygen concentration, hypoxia, intercellular space 相似文献
17.
Susan E. Melzer Oliver A. Chadwick Anthony S. Hartshorn Lesego M. Khomo Alan K. Knapp Eugene F. Kelly 《Biogeochemistry》2012,108(1-3):317-334
The efficacy of higher plants at mining Si from primary and secondary minerals in terrestrial ecosystems is now recognized as an important weathering mechanism. Grassland ecosystems are a particularly large reservoir of biogenic silica and are thus likely to be a key regulator of Si mobilization. Herein, we examine the effects of parent material (basaltic and granitic rocks) on the range and variability of biogenic silica pools in grass-dominated ecosystems along two precipitation gradients of Kruger National Park, South Africa. Four soil pedons and adjacent dominant plant species were characterized for biogenic silica content. Our results indicate that although soils derived from basalt had less total Si and dissolved Si than soils derived from granite, a greater proportion of the total Si was made up of biogenically derived silica. In general, plants and soils overlying basaltic versus granitic parent material stored greater quantities of biogenic silica and had longer turnover times of the biogenic silica pool in soils. Additionally, the relative abundance of biogenic silica was greater at the drier sites along the precipitation gradient regardless of parent material. These results suggest that the biogeochemical cycling of Si is strongly influenced by parent material and the hydrologic controls parent material imparts on soils. While soils derived from both basalt and granite are strongly regulated by biologic uptake, the former is a “tighter” system with less loss of Si than the latter which, although more dependent on biogenic silica dissolution, has greater losses of total Si. Lithologic discontinuities span beyond grasslands and are predicted to also influence biogenic silica cycling in other ecosystems. 相似文献
18.
Quantifying marine snow as a food choice for zooplankton using stable silicon isotope tracers 总被引:1,自引:0,他引:1
Aggregates of biogenic origin >0.5 mm, known as marine snow,represent a concentrated potential source of food for zooplankton.Little is known, however, about whether aggregates are commonlygrazed by zooplankton in the field. While previous laboratorystudies have shown that the euphausiid Euphausia pacifica, andthe copepod, Calanus pacificus, common crustacean zooplankters,consume marine snow if it is the only food source available,it is not known if euphausiids will select marine snow in thepresence of edible dispersed cells, as readily occurs in nature.To examine this question, we offered E. pacifica the diatom,Nitzschia angularis in aggregated and dispersed form as preysimultaneously. Aggregates and dispersed food contained identicalcell types so that differing prey quality, taste or motilitywould not be a factor. A new method was developed to track foodsources by labeling the frustules of aggregated cells with differentnaturally occurring, but rare, stable isotopes of silicon, 30Siand 29Si. Food selection was then estimated by measuring theisotopic composition of silica within fecal pellets producedby animals feeding on mixtures of the two labeled foods. Resultsindicate that E. pacifica consumed both aggregates and dispersedcells, even when more cells were made available in dispersedform than in aggregated form. This suggests that aggregatesmay indeed be a food source in the field, even when dispersedcells are relatively abundant. The method of labeling diatomcells with stable isotopes of silica may prove useful for futuregrazing experiments to distinguish identical cell types. 相似文献
19.
Silica deposits in young plants of Digitaria sanguinalis (L.)Scop., cultured in nutrient solutions containing two levelsof SiO2, were investigated by techniques including phase contrastmicroscopy and the electronprobe microanalyser. Field-grownspecimens were included in the study for comparison. Progressivesilicification over 20 days of early leaf ontogeny is describedand compared to that for other species. Si microassay results for culms and roots are combined withloeaf data in order to interpret overall deposition strategyfor the species. Comparisons between soil and water-cultured speciments suggestthat the extensive mesophyll silicification occurring in thelatter is atypical, and may influence growth. An attempt ismade to account for this latter silicification in terms of Kranzleaf anatomy. 相似文献
20.
Compactin (ML-236B), a specific inhibitor of 3-hydroxy-3-methylglutarylCoA reductase, inhibited the elongation of roots and hypocotylsof Medicago sativa seedlings when it was applied to the roots.Addition of mevalonic acid, the direct product of the enzyme,together with compactin relieved the growth inhibition of roots. The contents and compositions of sterols were studied in threeparts of M. sativa seedlingsroots, hypocotyls and cotyledons.Compactin (20 µM) decreased the sterol contents of rootsand hypocotyls by about a half but did not affect that of cotyledons.On the other hand, mevalonic acid (2 mM) increased the sterolcontent of roots more than threefold the nontreated controllevel but not the contents of hypocotyls and cotyledons. Mevalonicacid added in combination with compactin had a similar effecton the sterol content of roots as when it was added alone. The major sterol in all three parts was stigmasterol whetheror not compactin or mevalonic acid was present. However, thevariation of the sterol composition in the roots was distinct;mevalonic acid-treated roots markedly accumulated 7-sitosterol,24-methylenecycloartanol and squalene. (Received October 16, 1986; Accepted April 2, 1987) 相似文献