SPF大白猪和长白猪群体生理生化特性分析

目的研究分析SPF大白猪、长白猪生殖生理和血液生理生化特性。方法常规方法测定SPF大白猪、长白猪群体10项生殖生理指标、19项血液生理和18项血液生化指标,统计分析各指标在不同群体、不同年龄、不同性别间的差异。结果 SPF大白猪、长白猪生殖生理指标和血液大部分生理生化指标差异不显著(P0.05),个别指标如血液生理指标GRAN、HGB、RDW、PLT、PCT,血液生化指标ALKP、CHOL、TBIL、BUN在品种间、不同年龄以及不同性别之间差异显著(P0.05),但其偏离正常值范围较小。结论 SPF大白猪、长白猪在生殖生理和血液生理生化等方面基本稳定,与其他实验用小型猪相比差异不大,本研究为其标准化饲养、动物模型的构建以及封闭群猪群遗传质量的评价提供了重要的基础数据。     

不同性别成年四川白兔血液生理生化指标的研究

目的:为给成年四川白兔的肉质研究、保种育种、饲养管理和临床诊疗提供依据,从血液理化特性等方面研究四川白兔的种质特征。方法:选择健康成年四川白兔20只,雌雄各半,体重1.5~3 Kg,在普通环境中饲养观察一周,试验前禁食12h,自由饮水,清晨空腹耳缘静脉采血,采用全自动血液分析仪和全自动生化仪对成年四川白兔的血液生理生化指标进行测定,并将雌雄四川白兔血液生理生化指标进行比较。结果:血液生理指标中只有血小板表现为雌雄差异显著(P0.05),其他指标均差异不显著(P0.05);雌雄四川白兔生化指标中,碱性磷酸酶、总胆固醇、肌酐、差异显著(P0.05),其他指标均差异不显著(P0.05)。结论:所测成年四川白兔各项血液生理生化指标雌雄间多数指标差异不显著,说明了成年四川白兔雌雄间多数生理生化值基本一致,不受性别因素的影响。     

宁乡花猪原代和第一代血液生理生化指标比较

目的比较原代宁乡花猪与其第一代仔猪血液生理生化指标的变化。方法常规方法测定宁乡花猪F0代16头,F1代16头血液18项生理指标和13项生化指标。结果F0代与F1代部分血液生理指标如白细胞（WBC）、红细胞（RBC）、血红蛋白（HGB）、红细胞积压（HCT）等13项有显著性差异（P〈0．05）;部分血液生化指标如总胆红素（TBIL）,尿素氮（BUN）,总蛋白（TP）,白／球蛋白（ALB／GLB）,葡萄糖（GLU）,有显著性差异（P〈0．05）;其他指标均无统计学差异。结论2代宁乡花猪之间部分血液生理生化指标有显著性差异。得到的数据可以为实验动物化研究提供相应的基础生理参考数据。     

笼养绿尾虹雉血液生理生化指标分析

为了解人工饲养绿尾虹雉(Lophophorus lhuysii)血液生理生化指标及特点,以四川宝兴蜂桶寨国家级自然保护区笼养的9只成年非繁殖期绿尾虹雉为研究对象,测定了34项血液生理生化指标,按性别分组统计进行差异显著性检验。结果表明,绿尾虹雉血液生理指标不同性别间无显著差异(P0.05)。血液生化指标中的血液总蛋白雄性显著低于雌性(P0.05),血清尿素雄性显著高于雌性(P0.05),但血液中的其他有机物(白蛋白、球蛋白、肌酐、葡萄糖、尿素、酶、总胆固醇、甘油三酯等)及无机离子(钾、钠、镁、氯、钙、磷、铁)不同性别之间无显著差异(P0.05)。说明本实验条件下,笼养绿尾虹雉非繁殖期血液生理指标与性别没有相关性,血清总蛋白、血清尿素含量可能与性别有关。     

封闭群五指山小型猪血液生理生化指标的测定

目的对2-12月龄封闭群五指山小型猪的部分血液生理和生化指标进行测定和分析。方法常规方法测定五指山小型猪血液的19项生理和12项生化指标,统计分析各指标间的性别差异,并与近交系五指山小型猪以及其他小型猪的同类指标进行对比分析。结果封闭群五指山小型猪绝大多数血液生理、生化指标性别间差异不显著,仅生理指标中的粒细胞分类计数和生化指标中的总胆固醇差异显著。同近交系五指山小型猪同类指标比较,生理指标中血红蛋白和血小板相关指标存在差异,生化指标中有6项差异显著。同其他种类小型猪同类指标差异较与近交系五指山小型猪间差异明显。结论封闭群五指山小型猪血液生理、生化指标稳定,许多生理生化指标接近人类,可能逐步替代犬、猴用于药物非临床安全评价和生物医学研究。     

幼狼血液生理生化及激素参考值

为了了解狼(Canis lupus)幼体的血液生理生化和某些激素正常值及其特点,为相关生物学研究、人工饲养和健康检测提供参考资料,本文采用全自动血球计数仪、全自动生化分析系统和全自动第三代发光免疫仪对8只4月龄幼狼的40项血液生理生化及8项血清激素指标进行了测定.结果表明,雌雄幼狼的血液生理生化指标无显著差异;雄性幼狼的大多数血液理化指标均比雌性幼狼偏高;雌雄幼狼的血清激素水平存在一定的性别专属.     

白暨豚某些血液生化指标的测定

关于海豚和几种淡水豚类的血液学和血液生化指标国外有过研究。白暨豚(Lipotes vexillifer)血液有形成份已有过报道。本实验对白暨豚血液生化指标进行了研究,以期建立白暨豚血液正常生理生化指标,为白暨豚的临床诊断,健康监测和保健措施提供血液学参数,并且为完善淡水豚类血液学比较研究提供基础资料。     

雌雄金黄地鼠血液生理生化指标的比较分析

目的测定金黄地鼠的血液生理生化参考值,并对两性的参考值进行比较。方法将88只4周龄金黄地鼠,80只8周龄金黄地鼠,采血后,用日本光电MEK-7222K血球分析仪测定血液生理指标,用Olympus AU400全自动生化分析仪测定血清生化指标。结果4周龄金黄地鼠血液生理指标中RBC、HGB、HCT、MCV和RDW性别差异有显著性（P〈0.05）,而WBC、MCH、MCHC、PLT、PCT、MPV和PDW等性别差异无显著性（P〉0.05）;4周龄金黄地鼠血清生化指标中ALB、ALP、GLU、CA、P、CHO和TG性别差异有显著性（P〈0.05）,而ALT、AST、TP、BUN和CREA等性别差异无显著性（P〉0.05）。8周龄金黄地鼠血液生理指标中WBC、LYM、MON、BAS、LYM％、NEUT％、MCH、RBC、HGB、HCT、MCV、PDW和RDW性别差异有显著性（P〈0.05）,而MCHC、PLT、MPV、PCT、NEUT、EOS、BAS、MON％和EOS％等性别差异无显著性（P〉0.05）;8周金黄地鼠血清生化指标中ALT、ALB、BUN、GLU、CREA、P、CHO和TG性别差异有显著性（P〈0.05）,而AST、TP、ALP和CA等性别差异无显著性（P〉0.05）。结论金黄地鼠血液的生理生化值受多种因素影响,部分指标两性问测定值有差异,但多数指标差异不显著。     

喜马拉雅旱獭血液生理指标测定

首次测定了45只人工饲养条件下喜马拉雅旱獭的血液生理值.结果显示人工饲养条件下除淋巴细胞和中性粒细胞百分率因动物年龄不同而有显著性差异外(P<0.05),不同性别、不同饲养时间喜马拉雅旱獭的白细胞数、白细胞分类计数百分率、红细胞、血小板、血红蛋白和平均血红蛋白浓度等7项指标都没有显著性差异,说明建立的旱獭人工饲养管理方法可保证动物的血液生理指标的稳定性,所测血液生理指标也可作为喜马拉雅旱獭饲养管理和疫病检测判断的参考依据.     

清洁级SD大鼠血液生理生化指标的测定

目的建立清洁级SD大鼠血液生理生化正常值。方法应用动物芯片血球计数仪测试常规血细胞计数;采用全自动生化测定仪对清洁级SD大鼠的血液28项生化指标进行测定,并进行统计分析雌雄差异显著性检验。结果建立了清洁级SD大鼠血液生理和28项血液生化参考值,ALB、AST/ALT、AG雌雄之间差异显著;血液血球计数雌雄差异不显著。结论比较详细的建立了清洁级SD大鼠的血液生理生化指标参考值,为其应用提供基础数据。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.