Luminescence Literature

The journal provides comprehensive literature searches on all aspects of luminescence which will be published in most issues. These lists have been produced regularly since 1986—some by year and some by specialized topic. This survey of papers on green fluorescent protein and imaging is drawn from the 2000 literature and covers reagents, assay development and validation, and applications and supplements the previous survey (J. Biolumin. Chemilumin. 1997; 12 : 113–34). The 2000 Literature Compiled by L. J. Kricka (kricka@mail.med.upenn.edu) and P. E. Stanley (stanley@lumiweb.com)     

Luminescence Literature

The journal provides comprehensive literature searches on all aspects of luminescence which will be published in most issues. These lists have been produced regularly since 1986—some by year and some by specialized topic. This survey of papers on Obelia, Renilla, Aequorea and Gonyaulax is drawn from the 2000 literature and covers the biology of these organisms and the applications of genes and proteins from these organisms in analysis.     

Literature on archaeological remains of cultivated plants (1999/2000)

Publications on archaeological remains of cultivated plants have been collected, mainly from 1999, with some earlier and some from 2000. A list is given of the finds according to taxon, country, site, and age. Received November 22, 2000 / Accepted November 27, 2000     

Luminescence biosensors

A novel optical biosensor for homogeneous immunoassay has been developed on the basis of the finding that electrochemical luminescence of pyrene-labelled antigen is extremely inhibited by immunochemical complexation. Electrochemical luminescence homogeneous immunoassay for human serum albumin (HSA), as a model analyte, was performed with a platinum plate electrode which was located in the vicinity of an optical fibre tip. HSA was determined in the concentration range of 3–25 × 10^?6 mol/I. To improve electrochemical luminescence measurement an optical fibre electrode has been developed by fabricating a transparent platinum film on the top of an optical fibre. The minimum detectable limit of luminol was 10^?11 mol/l with the optical fibre electrode. Luminol was applied as a label for homogeneous immunoassay.     

稀土发光材料在荧光成像中的应用

稀土发光材料由于具有荧光寿命长、发射峰半峰宽窄和Stokes位移大等发光性质,在生命科学研究的各个领域,包括荧光免疫分析、离子识别、蛋白质活性测定、核酸检测等,有着广泛而重要的应用前景.本文以稀土配合物、稀土掺杂上转换材料和长余辉材料为代表,就当前稀土发光材料的发光性质及其在生物成像标记方面的研究做一综述,并对稀土发光...     

Luminescence of activated phagocytes

By means of microscope-cytofluorometry "Luman-13" intensive proper luminescence of neutrophilic granulocytes and monocytes in blood and pus, that reduce nitroblue tetrazolium has been investigated. The luminescence is localized in the cytoplasmic areas free from formazan; formazan itself does not demonstrate any luminescence. Two types of luminescence have been revealed: dark blue-blue and yellow-green. According to the luminescence spectral analysis stimulation with radiation at the wave length 360 nm produces luminescence of the I type with its maximum in the area of 450-470 nm and 520-535 nm. When stimulation with light at the wave length is 460 nm, the luminescence spectrum++ of the II type possesses the main maximum at 535 nm. Luminescence spectra of neutrophilic granulocytes and monocytes are similar. The luminescence stripe in the area 450-470 nm results from reduced pyridinenucleotides, luminescence with the wave length 520-535 nm--from oxidized flavoproteins.     

Luminescence of deoxyhemocyanin and deoxytyrosinase

The deoxy form of hemocyanins and tyrosinases from certain species displays a weak low-energy luminescence when solutions of the protein are irradiated with light at approximately 290 nm. The emission most likely results from a copper-to-imidazole charge transfer state as shown by studies with a synthetic copper(I) complex having three imidazole ligands.