Is there an interchromosomal effect in reciprocal translocation carriers? Sperm FISH studies

Chromosome translocations have been known to affect disjunction of chromosomes unrelated to the translocation in the mouse and in Drosophila. However, in humans, an interchromosomal effect in chromosome translocations has not been demonstrated. The availability of techniques that allow the study of nondisjunction in sperm cells has permitted us to evaluate the possibility of an interchromosomal effect in male translocation heterozygotes. In this study, multicolor fluorescence in situ hybridization was used to determine levels of disomy for the clinically relevant chromosomes X, Y, 13, 18, and 21 in 332,858 spermatozoa from nine reciprocal translocation heterozygotes and nine controls with normal karyotypes. The specific translocations studied were as follows: t(10;12)(p26.1;p13.3), t(2;18)(p21;q11.2), t(3;19)(p25;q12), t(5;8)(q33;q13), t(11;22)(q23;q11), t(3;4)(p25;p16), t(8;9) (q24.2;q32), t(10;18)(q24.1;p11.2), and t(4;10)(q33;p12.2). Comparisons of disomy rates between carriers and controls were performed by using the Mann-Whitney test. Our results showed that the rates of sex chromosome hyperhaploidy were similar in controls (0.21%) and in translocation carriers (0.19%). Similarly, the frequencies of disomy for chromosomes 13, 18, and 21 did not differ significantly between controls and carriers (0.05% versus 0.08%, 0.07% versus 0.03%, and 0.14% versus 0.20%, respectively). Sex chromosome nondisjunction was more common than nondisjunction of chromosomes 13 and 18 both in controls (P=0.0057) and in carriers (P=0.0008). Similarly, the rates of chromosome disomy for chromosome 21 were higher than those for chromosomes 13 and 18 in both controls (P=0.0031) and translocation carriers (P=0.0057). In our study, the excess of chromosome 21 disomy versus disomy of the other autosomes was more pronounced in carriers than in controls. Thus, although the difference of disomy 21 between controls and carriers was not statistically significant, it is worthy of attention.     

Is there an urban effect in alien plant invasions?

Cities are known to be extraordinarily rich in alien plant species compared to rural environments. This is related to specific attributes of urban areas including the availability of natural resources and habitats (namely geological substrates and land cover), the dispersal pathways and associated propagule pressure due to trade and traffic, and the proximity many urban hubs have to rivers. Here we explored how richness and proportions of alien species introduced after the discovery of the Americas (so-called neophytes), can be explained by environmental covariates along the urbanization gradient from very rural to very urbanized grid cells. We tested whether there is a specific urban effect, either as an interaction effect of urbanized areas that changes these general relationships, or if there is an effect due to specific urban conditions. We found that the environmental covariates explaining richness as well as proportions of neophytes remain largely the same across the rural–urban gradient. There is, however, an effect of urbanized area on neophyte species richness and proportions, which also incorporates strictly urban conditions. Rivers, roads and railroads contribute disproportionately less to the increase of neophyte species diversity in more urbanized areas, which might be due to the already higher number of neophytes in cities. We argue that the conditions determining neophyte richness in cities are not fundamentally different from those in rural environments, but extend on the same environmental axis, i.e. having different positions along the gradient towards the upper end.     

Is there an alternative to increasing the sample size in microarray studies?

Our answer to the question posed in the title is negative. This intentionally provocative note discusses the issue of sample size in microarray studies from several angles. We suggest that the current view of microarrays as no more than a screening tool be changed and small sample studies no longer be considered appropriate.     

Is there a Root effect in Xenopus hemoglobin?

The reaction of Xenopus hemoglobin with oxygen and carbon monoxide has been reinvestigated over the pH range 8.5-6.0, in the absence and presence of organic phosphates (2,3-diphosphoglycerate or inositol hexakisphosphate), to establish if the tetramer can be stabilized in a T-quaternary state by protons and polyphosphate; the equilibrium and kinetic data indicate that Xenopus hemoglobin does exhibit a Root effect. These new results are discussed with reference to those reported by Bridges et al. [(1985) Resp. Physiol. 61, 125-136] on Xenopus blood and, more generally, to the molecular definition and the structural basis of the Root effect as an extreme form of the Bohr effect.     

Is there an adverse effect of sons on maternal longevity?

Recent years have witnessed the emergence of a literature examining the effects of giving birth to sons on postmenopausal longevity in pre-industrial mothers. The original paper in this lineage used a sample (n=375) of Sami mothers from northern Finland and found that, relative to daughters, giving birth to sons substantially reduced maternal longevity. We examine this hypothesis using a similar and a much larger sample (n=930) of pre-industrial Sami women from northern Sweden, who in terms of their demographic, sociocultural and biological conditions, closely resemble the original study population. In contrast to the previously reported results for the Sami, we find no evidence of a negative effect of sons on maternal longevity. Thus, we provide the most compelling evidence to date that the leading result in the literature must be approached with scepticism.     

Is there plasticity in developmental instability? The effect of daily thermal fluctuations in an ectotherm

Diversified bet‐hedging (DBH) by production of within‐genotype phenotypic variance may evolve to maximize fitness in stochastic environments. Bet‐hedging is generally associated with parental effects, but phenotypic variation may also develop throughout life via developmental instability (DI). This opens for the possibility of a within‐generation mechanism creating DBH during the lifetime of individuals. If so, DI could in fact be a plastic trait itself; if a fluctuating environment indicates uncertainty about future conditions, sensing such fluctuations could trigger DI as a DBH response. However, this possibility has received little empirical attention. Here, we test whether fluctuating environments may elicit such a response in the clonally reproducing crustacean Daphnia magna. Specifically, we exposed genetically identical individuals to two environments of different thermal stability (stable vs. pronounced daily realistic temperature fluctuations) and tested for effects on DI in body mass and metabolic rate shortly before maturation. Furthermore, we also estimated the genetic variation in DI. Interestingly, fluctuating temperatures did not affect body mass, but metabolic rate decreased. We found no evidence for plasticity in DI in response to environmental fluctuations. The lack of plasticity was common to all genotypes, and for both traits studied. However, we found considerable evolvability for DI, which implies a general evolutionary potential for DBH under selection for increased phenotypic variance.     

Is there an inner nose?

Although behavioral and neuropsychological data regarding the existence of images for odors are inconclusive, reconsideration of earlier EEG work provides reasonably clear evidence for an inner nose. However, further EEG studies and neuroimaging data seem essential for conclusive demonstration of an inner nose.      

Is there an Asian bioethics?

Japan and China have long resisted the Western trend of organ transplantation from brain-dead patients, based on a 'Confucian' respect for integrity of ancestors' bodies. While their general publics continue to harbor grave doubts about such practices, their medical and political elites are hastening towards the road of organ-harvesting and organ-marketing, largely for economic reasons. This report illustrates the ways that economics is motivating brain-death legislation in Japan and criminal executions in China.     

Is there an interleukin 2 inhibitor in human serum?

Normal mouse serum has been shown to contain an inhibitor of interleukin 2 (IL-2). Here we report that a molecule with similar activity cannot be found in normal human serum (NHS). Although NHS inhibited the IL-2-dependent proliferation of mouse CTLL cells, as expected of an IL-2 inhibitor, it also had inhibitory activity on IL-3-dependent cells and was cytolytic to IL-2-independent mouse cells as measured by a ⁵¹Cr release assay, indicating a nonspecific effect. In addition, NHS had no effect on the IL-2-dependent proliferation of human peripheral blood T-cell blasts. Fractionation of NHS by size exclusion HPLC failed to separate cytolytic activity from any putative true IL-2 inhibitor activity. The cytolytic component was not related to immunoglobulin since it had a molecular weight of 50,000 to 60,000 and was not bound by protein-A-Sepharose. However, its molecular weight, heat lability, and trypsin sensitivity suggest it to be a protein.     

Is there an estrogenic component in the metabolic syndrome?

One of the major upcoming concerns leading to health related problems in the industrialized societies is the metabolic syndrome which is characterized by central obesity, hypertension, raised fasting glucose and triglyceride levels. The focus of this review is on a potential estrogenic linkage between the metabolic mechanisms involved into the development of this disease cluster and specific estrogen related regulatory pattern. The candidate molecules for this link are insulin and insulin-like growthfactor, C-reactive protein, peroxisome-proliferation-activatingreceptorgamma, and leptin which all seem to interact with each other and show a responsiveness to changing estrogen levels. From this perspective they might also represent target molecules for a phytochemical intervention with phytoestrogens.