共查询到20条相似文献,搜索用时 62 毫秒
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Shin Takato Yusuke Kakei Marie Mitsui Yosuke Ishida Masashi Suzuki Chiaki Yamazaki 《Bioscience, biotechnology, and biochemistry》2017,81(7):1320-1326
We previously reported that exogenous application of auxin to Arabidopsis seedlings resulted in downregulation of indole-3-acetic acid (IAA) biosynthesis genes in a feedback manner. In this study, we investigated the involvement of the SCFTIR1/AFB-mediated signaling pathway in feedback regulation of the indole-3-pyruvic acid-mediated auxin biosynthesis pathway in Arabidopsis. Application of PEO-IAA, an inhibitor of the IAA signal transduction pathway, to wild-type seedlings resulted in increased endogenous IAA levels in roots. Endogenous IAA levels in the auxin-signaling mutants axr2-1, axr3-3, and tir1-1afb1-1afb2-1afb3-1 also increased. Furthermore, YUCCA (YUC) gene expression was repressed in response to auxin treatment, and expression of YUC7 and YUC8 increased in response to PEO-IAA treatment. YUC genes were also induced in auxin-signaling mutants but repressed in TIR1-overexpression lines. These observations suggest that the endogenous IAA levels are regulated by auxin biosynthesis in a feedback manner, and the Aux/IAA and SCFTIR1/AFB-mediated auxin-signaling pathway regulates the expression of YUC genes. 相似文献
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Ginis O Courdavault V Melin C Lanoue A Giglioli-Guivarc'h N St-Pierre B Courtois M Oudin A 《Molecular biology reports》2012,39(5):5433-5447
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A. M. Middleton J. R. King M. J. Bennett M. R. Owen 《Bulletin of mathematical biology》2010,72(6):1383-1407
The hormone auxin is implicated in regulating a diverse range of developmental processes in plants. Auxin acts in part by
inducing the Aux/IAA genes. The associated pathway comprises multiple negative feedback loops (whereby Aux/IAA proteins can repress Aux/IAA genes) that are disrupted by auxin mediating the turnover of Aux/IAA protein. In this paper, we develop a mathematical model
of a single Aux/IAA negative feedback loop in a population of identical cells. The model has a single steady-state. We explore parameter space
to uncover a number of dynamical regimes. In particular, we identify the ratio between the Aux/IAA protein and mRNA turnover
rates as a key parameter in the model. When this ratio is sufficiently small, the system can evolve to a stable limit cycle,
corresponding to an oscillation in Aux/IAA expression levels. Otherwise, the steady-state is either a stable-node or a stable-spiral. These observations may shed light
on recent experimental results. 相似文献
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Auxin action in a cell-free system 总被引:24,自引:0,他引:24
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Roles and activities of Aux/IAA proteins in Arabidopsis. 总被引:12,自引:0,他引:12
J W Reed 《Trends in plant science》2001,6(9):420-425
Auxin induces various distinct developmental responses, partly by regulating gene expression. The Aux/IAA genes are a large gene family, many of which are induced by auxin. Work on Arabidopsis Aux/IAA genes has begun to reveal that they can regulate development and auxin-induced gene expression. Furthermore, auxin responses require Aux/IAA protein turnover. Finally, recent evidence suggests that Aux/IAA proteins can mediate light responses. Work in the near future should test whether Aux/IAA proteins are antennae that connect auxin and light signals to endogenous developmental responses. 相似文献
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Auxin receptors: a new role for F-box proteins 总被引:15,自引:0,他引:15
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Overexpression of the non-canonical Aux/IAA genes causes auxin-related aberrant phenotypes in Arabidopsis 总被引:2,自引:0,他引:2
Degradation of Aux/IAA proteins which are triggered by the ubiquitin ligase complex containing the auxin F-box receptors (AFBs), is thought to be the primary reaction of auxin signaling. Upon auxin perception, AFBs bind domain II of Aux/IAA proteins that is conserved in most of the 29 family members in Arabidopsis. However, IAA20 and IAA30 lack domain II. Furthermore, IAA31, which forms a single clade with IAA20 and IAA30 in Aux/IAA protein family, has a partially conserved domain II, which contains an amino acid substitution that would cause a dominant mutation of Aux/IAA genes. It has been shown that the half-lives of these proteins are much longer than those of the canonical Aux/IAA proteins. We generated overexpression lines (OXs) of IAA20 , IAA30 and IAA31 by the use of cauliflower mosaic virus 35S promoter to better understand the molecular function of atypical Aux/IAA proteins in Arabidopsis. OXs of the three genes exhibited similar auxin-related aberrant phenotypes, with IAA20 OX showing the most severe defects: Some of them showed a semi-dwarf phenotype; gravitropic growth orientation was often affected in hypocotyl and root; vasculature of cotyledons was malformed; the primary root stopped growing soon after germination because of collapse of root apical meristem. IAA 20 and IAA30 were early auxin inducible, but IAA31 was not. These results showed that the wild-type genes of the three Aux/IAAs could disturb auxin physiology when ectopically overexpressed. 相似文献
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Sarma Rajeev Kumar Avanish Rai Dikki Pedenla Bomzan Krishna Kumar Andra Hemmerlin Varun Dwivedi Rucha C. Godbole Vitthal Barvkar Karuna Shanker H. B. Shilpashree Ankita Bhattacharya Attibele Ramamurthy Smitha Namratha Hegde Dinesh A. Nagegowda 《The Plant journal : for cell and molecular biology》2020,103(1):248-265