共查询到20条相似文献,搜索用时 15 毫秒
1.
S. Van Campenhout L. Sági J. Vander Stappen G. Volckaert 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):80-86
DNA sequences encoding type-I thionins were isolated from Triticum aestivum L. cv ‘Chinese Spring’ using PCR with consensus primers. Blunt-end cloning, sequencing and PCR-based chromosome assignment
of these fragments uncovered the three orthologous sequences corresponding to the single-copy genes at the Pur-1 loci on each of the group-1 chromosomes. Comparison with two previously published cDNA sequences revealed the presence of
two introns that contain most of the polymorphic nucleotide sites. The observed orthologous DNA sequence variation among Pur-1 loci, encoded by each of the A, B and D genomes, enabled us to establish interlocus relationships and to construct locus-specific
primer sets. Analogously, the Pur-R1 sequence from rye was isolated, and a locus-specific primer pair was constructed as well. Hence, four locus-specific primer
sets are now available as molecular markers for the homoeologous 1AL, 1BL, 1DL and 1RL chromosome arms. Amplification from
several diploid and tetraploid wheat species showed that the primers can be used as molecular tools for studying wheat phylogeny.
Received: 30 January 1997 / Accepted: 23 June 1997 相似文献
2.
G. J. Bryan A. J. Collins P. Stephenson A. Orry J. B. Smith M. D. Gale 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(5):557-563
The development of large panels of simple-to-analyse genetic markers for tagging agronomically important genes and diversity
studies in hexaploid bread wheat is an important goal in applied cereal genetic research. We have isolated and sequenced over
200 clones containing microsatellites from the wheat genome and have tested 153 primer pairs for genetic polymorphism using
a panel of ten wheat varieties, including the parents of our main mapping cross. A subset comprising 49 primer pairs detects
76 loci, of which 74 can be unequivocably allocated to one of the wheat chromosomes. A relatively low frequency of the loci
detected are from the D genome, and these loci show less polymorphism than those from the A and B genomes. Generally, the
microsatellites show high levels of genetic polymorphism and an average of 3.5 alleles per locus with an average polymorphism
information content (PIC), value of 0.51. The observed levels of polymorphism are positively correlated with the length of
the microsatellite repeats. A high proportion, approximately two-thirds, of primer pairs designed to detect simple sequence
repeat (SSR) variation in wheat do not generate the expected amplification products and, more significantly, often generate
unresolvable PCR products. In general, our results agree closely with those obtained from other recent studies using microsatellites
in plants.
Received: 19 March 1996 / Accepted: 28 June 1996 相似文献
3.
Cloning and characterisation of a family of disease resistance gene analogs from wheat and barley 总被引:7,自引:0,他引:7
S. Seah K. Sivasithamparam A. Karakousis E. S. Lagudah 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):937-945
The most common class of plant disease resistance (R) genes cloned so far belong to the NBS-LRR group which contain nucleotide-binding
sites (NBS) and a leucine-rich repeat (LRR). Specific primer sequences derived from a previously isolated NBS-LRR sequence
at the Cre3 locus, which confers resistance to cereal cyst nematode (CCN) in wheat (Triticum aestivum L.) were used in isolating a family of resistance gene analogs (RGA) through a polymerase chain reaction (PCR) cloning approach.
The cloning, analysis and genetic mapping of a family of RGAs from wheat (cv ‘Chinese Spring’) and barley (Hordeum vulgare L. cvs ‘Chebec’ and ‘Harrington’) are presented. The wheat and barley RGAs contain other conserved motifs present in known
R genes from other plants and share between 55–99% amino acid sequence identity to the NBS-LRR sequence at the Cre3 locus. Phylogenetic analysis of the RGAs with other cloned R genes and RGAs from various plant species indicate that they
belong to a superfamily of NBS-containing genes. Two of the barley derived RGAs were mapped onto loci on chromosomes 2H (2),
5H (7) and 7H (1) using barley doubled haploid (DH) mapping populations. Some of these loci identified are associated with
regions carrying resistance to CCN and corn leaf aphid.
Received: 6 January 1998 / Accepted: 1 April 1998 相似文献
4.
J. H. Peng T. Fahima M. S. Röder Y. C. Li A. Dahan A. Grama Y. I. Ronin A. B. Korol E. Nevo 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):862-872
Stripe rust caused by Puccinia striifomis West. is one of the most devastating diseases relating to wheat production. Wild emmer wheat, Triticum dicoccoides, the tetraploid progenitor of cultivated wheat, has proven to be a valuable source of novel stripe-rust resistance genes
for wheat breeding. For example, T. dicoccoides accessions from Mt. Hermon, Israel, are uniformly and highly resistant to stripe-rust. The main objective of the present
study is to map a stripe-rust resistance gene, derived from the unique Mt. Hermon population of wild emmer, using microsatellite
markers. An F2 mapping population was established by crossing stripe-rust resistant T. dicoccoides accession H52 from Mt. Hermon with the Triticum durum cultivar Langdon. The stripe-rust resistance derived from accession H52 was found to be controlled by a single dominant gene
which was temporarily designated as YrH52. Out of 120 microsatellite markers tested, 109 (91%) showed polymorphism between the parental lines. Among 79 segregating
microsatellite loci generated from 56 microsatellite primer pairs, nine were linked to YrH52 with recombination frequencies of 0.02–0.35, and LOD scores of 3.56–54.22. A genetic map of chromosome 1B, consisting of
ten microsatellite loci and the stripe-rust resistance gene YrH52, was constructed with a total map length of 101.5 cM. YrH52 is also closely linked to RFLP marker Nor1 with a map distance of 1.4 cM and a LOD value of 29.62. Apparent negative crossover interference was observed in chromosome
1B, especially in the region spanning the centromere. Negative crossover interference may be a common characteristic of gene-rich
regions or gene clusters in specific chromosomes.
Received: 30 October 1998 / Accepted: 2 November 1998 相似文献
5.
Katherine P. Munkres Line K. Bay Dean R. Jerry Mark I. McCormick Lynne Van Herwerden 《Conservation Genetics》2007,8(4):987-990
Five new polymorphic microsatellite loci were developed for the coral reef damselfish Pomacentrus amboinensis. Twenty-four individuals from two Great Barrier Reef populations were genotyped at the five loci, with numbers of alleles
per locus ranging from 6–23 and observed heterozygosity between 0.42–0.92. In addition, the cross-species testing of six primers
developed for Stegastes partitus revealed one primer (SpGATA40) that was also polymorphic for P. amboinensis. Due to high levels of polymorphism (≥14 alleles) in at least four of the six loci and a high proportion of tetranucleotide
repeats, these microsatellite markers should be useful for parentage assignment as well as other investigations of individual
relatedness. 相似文献
6.
A genetic linkage map of tef [Eragrostis tef (Zucc.) Trotter] based on amplified fragment length polymorphism 总被引:2,自引:0,他引:2
G. Bai H. Tefera M. Ayele H. T. Nguyen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):599-604
A genetic linkage map of tef was constructed with amplified fragment length polymorphism (AFLP) markers using F5 recombinant inbred lines (RILs) derived by single seed descent from the intraspecific cross of ’Kaye Murri’×’Fesho’. A total
of 192 EcoRI/MseI primer combinations were screened for parental polymorphism. Around three polymorphic fragments per primer combination were
detected, indicating a low polymorphism level in tef. Fifty primer combinations were selected to assay the mapping population,
and 226 loci segregated among 85 F5 RILs. Most AFLP loci behaved as dominant markers (presence or absence of a band), but about 15% of the loci were codominant.
Significant deviations from the expected Mendelian segregation ratio were observed for 26 loci. The genetic linkage map comprised
211 markers assembled into 25 linkage groups and covered 2,149 cM of genome. AFLP is an efficient marker system for mapping
plant species with low polymorphism such as tef. This is the first genetic linkage map constructed for tef. It will facilitate
the mapping of genes controlling agronomically important traits and cultivar improvement in tef.
Received: 27 April 1998 / Accepted: 4 January 1999 相似文献
7.
8.
M. M. Messmer M. Keller S. Zanetti B. Keller 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1163-1170
We constructed a genetic map of a cross between the Swiss winter wheat (Triticum aestivum L.) variety Forno and the Swiss winter spelt (Triticum spelta L.) variety Oberkulmer. For the linkage analysis,176 polymorphic RFLP probes and nine microsatellites were tested on 204
F5 recombinant inbred lines (RILs) of Forno×Oberkulmer revealing 242 segregating marker loci. Thirty five percent of these loci
showed significant (P>0.05) deviation from a 1 : 1 segregation, and the percentage of Forno alleles ranged from 21% to 83% for individual marker
loci. Linkage analysis was performed with the program MAPMAKER using the Haldane mapping function. Using a LOD threshold of
10, we obtained 37 linkage groups. After finding the best order of marker loci within linkage groups by multi-point analysis
we assembled the linkage groups into 23 larger units by lowering the LOD threshold. All except one of the 23 new linkage groups
could be assigned to physical chromosomes or chromosome arms according to hybridisation patterns of nulli-tetrasomic lines
of Chinese Spring and published wheat maps. This resulted in a genetic map comprising 230 marker loci and spanning 2469 cM.
Since the analysed population is segregating for a wide range of agronomically important traits, this genetic map is an ideal
basis for the identification of quantitative trait loci (QTLs) for these traits.
Received: 3 August 1998 / Accepted: 28 November 1998 相似文献
9.
A Genetic linkage map of Pinyon pine (Pinus edulis) based on amplified fragment length polymorphisms 总被引:1,自引:0,他引:1
S. E. Travis K. Ritland T. G. Whitham P. Keim 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):871-880
Amplified fragment length polymorphisms (AFLP) were used to rapidly generate a dense linkage map for pinyon pine (Pinus edulis). The map population consisted of 40 megagametophytes derived from one tree at Sunset Crater, Arizona. A total of 78 primer
combinations, each with three to five selective nucleotides, amplified 542 polymorphic markers. Of these, 33 markers showed
significant deviation from the expected Mendelian genotypic segregation ratio of 1 : 1, and 164 showed complete linkage with
another marker. This resulted in 338 unique markers mapping to 25 linkage groups, each of which ranged from 2 to 22 markers,
averaging 80 centiMorgans (cM) in size and covering 2,012 cM (2,200 cM with the inclusion of 25 cM for each of 7 unlinked
markers). Pairwise linkage values gave a genome size estimate of 2,390 cM, suggesting comprehensive coverage of the genome.
A search for subsets of primer combinations giving the best map coverage found 10 primer combinations which together marked
72% of the linkage map to within 10 cM; an additional 10 primer combinations increased this percentage to 85%. Our map represents
an initial step towards the identification of quantitative trait loci associated with pest resistance and water stress in
pinyons and will further allow us to examine introgression rates between P. edulis and P. californiarum.
Received: 14 October 1997 / Accepted: 29 April 1998 相似文献
10.
EST derived SSR markers for comparative mapping in wheat and rice 总被引:18,自引:0,他引:18
Structural and functional relationships between the genomes of hexaploid wheat (Triticum aestivum L.) (2n=6x=42) and rice (Oryza sativa L.) (2n=2x=24) were evaluated using linkage maps supplemented with simple sequence repeat (SSR) loci obtained from publicly available expressed sequence tags (ESTs). EST-SSR markers were developed using two main strategies to design primers for each gene: (1) primer design for multiple species based on supercluster analysis, and (2) species-specific primer design. Amplification was more consistent using the species-specific primer design for each gene. Forty-four percent of the primers designed specifically for wheat sequences were successful in amplifying DNA from both species. Existing genetic linkage maps were enhanced for the wheat and rice genomes using orthologous loci amplified with 58 EST-SSR markers obtained from both wheat and rice ESTs. The PCR-based anchor loci identified by these EST-SSR markers support previous patterns of conservation between wheat and rice genomes; however, there was a high frequency of interrupted colinearity. In addition, multiple loci amplified by these primers made the comparative analysis more difficult. Enhanced comparative maps of wheat and rice provide a useful tool for interpreting and transferring molecular, genetic, and breeding information between these two important species. These EST-SSR markers are particularly useful for constructing comparative framework maps for different species, because they amplify closely related genes to provide anchor points across species.Communicated by R. Hagemann 相似文献
11.
Microsatellite-based molecular diversity of bread wheat germplasm and association mapping of wheat resistance to the Russian wheat aphid 总被引:1,自引:0,他引:1
Genetic diversity of a set of 71 wheat accessions, including 53 biotype 2 Russian wheat aphid (RWA2)-resistant landraces and
18 RWA2 susceptible accessions, was assessed by examining molecular variation at multiple microsatellite (SSR) loci. Fifty-one
wheat SSR primer pairs were used, 81 SSR loci were determined, and 545 SSR alleles were detected. These SSR loci covered all
the three genomes, 21 chromosomes, and at least 41 of the 42 chromosome arms. Diversity values averaged over SSR loci were
high with mean number of SSR alleles/locus = 6.7, mean Shannon’s index (H) = 1.291, and mean Nei’s gene diversity (He) = 0.609. The three wheat genomes ranked as A > D > B and the homoeologous groups ranked as 7 > 3 > 1 > 2 > 6 > 5 > 4 based
on the number of alleles per locus. Xgwm136 on chromosome arm 1AS is the most polymorphic SSR locus with the largest number of observed and effective alleles and the
highest H and He. Among all 2485 pairs of wheat accessions, genetic distance (GD) ranged from 0.054 to 1.933 and averaged 0.9832. A dendrogram
based on GD matrix showed that all the wheat accessions could be grouped into distinct clusters. Most of the susceptible cultivars
(13/18) were clustered into groups that contains all or mostly susceptible accessions. Most of the U.S. cultivars belong to
a group that is distinguishable from all the different RWA2 resistant groups. Diversity analysis was also conducted separately
for subgroups containing 53 RWA2-resistant accessions and 18 RWA2-susceptible accessions. Association mapping revealed 28
SSR loci significantly associated with leaf chlorosis, and 8 with leaf rolling. New chromosome regions associated with RWA2
resistance were detected, and indicated existence of new RWA resistance genes located on chromosomes of all other homoeologous
groups in addition to the groups 1 and 7 in bread wheat. This information is helpful for development of mapping populations
for RWA2 resistance genes from different phylogenetic groups, and for wise utilization of the RWA-resistant germplasm in wheat
breeding programs. 相似文献
12.
A genetic linkage map of durum wheat 总被引:20,自引:6,他引:14
A. Blanco M. P. Bellomo A. Cenci C. De Giovanni R. D’Ovidio E. Iacono B. Laddomada M. A. Pagnotta E. Porceddu A. Sciancalepore R. Simeone O. A. Tanzarella 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):721-728
A genetic linkage map of tetraploid wheat [Triticum turgidum (L.) Thell.] was constructed using segregation data from a population of 65 recombinant inbred lines (RILs) derived from
a cross between the durum wheat cultivar Messapia and accession MG4343 of T. turgidum (L.) Thell. ssp dicoccoides (Korn.) Thell. A total of 259 loci were analysed, including 244 restriction fragment length polymorphisms (RFLPs), one PCR
(polymerase chain reaction) marker (a sequence coding for a LMW (low-molecular-weight) glutenin subunit gene located at the
Glu-B3 locus), seven biochemical (six seed-storage protein loci and one isozyme locus) and seven morphological markers. A total
of 213 loci were mapped at a LOD≥3 on all 14 chromosomes of the A and B genomes. The total length of the map is 1352 cM and
the average distance between adjacent markers is 6.3 cM. Forty six loci could not be mapped at a LOD≥3. A fraction (18.6%)
of the markers deviated significantly from the expected Mendelian ratios; clusters of loci showing distorted segregation were
found on chromosomes 1B, 3AL, 4AL, 6AL and 7AL. The durum wheat map was compared with the published maps of bread wheat using
several common RFLP markers and general features are discussed. The markers detected the known structural rearrangements involving
chromosomes 4A, 5A and 7B as well as the translocation between 2B-6B, but not the deletion on 2BS. This map provides a useful
tool for analysing and breeding economically important quantitative traits and for marker-assisted selection, as well as for
studies of genome organisation in small grain cereal species.
Received: 5 January 1998 / Accepted: 31 March 1998 相似文献
13.
Development of microsatellite markers from tartary buckwheat 总被引:2,自引:0,他引:2
A genomic library enriched with (gT)n repeats from tartary buckwheat (Fagopyrum tataricum) was constructed using 5′-anchored PCR for the development of microsattellite markers. Sequencing analysis of 5 clones from
the library showed that they all contained microsatellites (totally 10 loci), and each was unique. An additional locus-specific
primer was designed according to flanking sequence. Two of the microsatellite loci of 10 tartary buckwheat varieties were
amplified using an anchored primer and a locus-specific primer, which revealed a clear polymorphic pattern. The data confirmed
that the degenerate primer was reliably anchoring at the 5′-end of the microsatellite, and the primers developed based on
this technology could be used for diversity analysis of tartary buckwheat. 相似文献
14.
P. J. Fisher T. E. Richardson R. C. Gardner 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):969-979
Dinucleotide microsatellites were isolated from Pinus radiata using both a standard genomic library and libraries enriched for microsatellites. Locus-specific primers were designed to
amplify 43 unique microsatellites. Thirty two of these loci had interpretable PCR patterns, 11 of which were polymorphic in
a screen of 19 P. radiata individuals; all 11 polymorphic loci contained at least 17 repeats in the sequenced plasmid. Six of the eleven primer pairs
amplified multiple fragments per individual (3–8), suggesting that these loci were present in multiple copies in the genome.
Genotyping a 48-tree P. radiata production population with seven of the most polymorphic microsatellites revealed an average of 17 bands per locus (the multi-copy
microsatellites were treated as one locus). When tested on known pedigrees, both single and multi-copy microsatellites exhibited
co-dominant inheritance and Mendelian segregation. Two loci had null alleles and one locus had a high frequency of non-parental
alleles, suggesting a high mutation rate. Eight of these microsatellites, including five multi-copy loci, were placed on a
partially constructed P. radiata genetic map. Four of the five multi-copy microsatellites had two or more sets of alleles that mapped to the same locus, and
the fifth mapped to two unlinked loci. All seven tested primer pairs amplified PCR products from other species of hard pine,
three amplified products from soft-pine species, and one amplified bands in other conifers.
Received: 10 November 1997 / Accepted: 5 January 1998 相似文献
15.
Microsatellite polymorphism in natural populations of wild emmer wheat,Triticum dicoccoides,in Israel 总被引:5,自引:0,他引:5
Fahima T Röder MS Wendehake K Kirzhner VM Nevo E 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(1):17-29
Diversity in 20 microsatellite loci of wild emmer wheat, Triticum dicoccoides, was examined in 15 populations (135 genotypes) representing a wide range of ecological conditions of soil, temperature, and
water availability, in Israel and Turkey. An extensive amount of diversity at microsatellite loci was observed despite the
predominantly selfing nature of this plant species. The 20 Gatersleben wheat microsatellites (GWM), representing 13 chromosomes
of genomes A and B of wheat, revealed a total of 364 alleles, with an average of 18 alleles per GWM marker (range: 5–26).
The proportion of polymorphic loci per population averaged 0.90 (range: 0.45– 1.00); genic diversity, He, averaged 0.50 (range 0.094– 0.736); and Shannon’s information index averaged 0.84 (range 0.166–1.307). The coefficients
of genetic distance between populations were high and averaged D=1.862 (range 0.876–3.320), an indication of sharp genetic divergence over short distances. Interpopulation genetic distances
showed no association with geographic distance between the population sites of origin, which ruled out a simple isolation
by distance model. Genetic dissimilarity values between genotypes were used to produce a dendrogram of the relationships among
wild wheat populations by the unweighted pair-group method with arithmetic averages (UPGMA). The results showed that all the
wild emmer wheat populations could be distinguished. Microsatellite analysis was found to be highly effective in distinguishing
genotypes of T. dicoccoides, originating from diverse ecogeographical sites in Israel and Turkey, with 88% of the 135 genotypes correctly classified into
sites of origin by discriminant analysis. Our present microsatellite results are non-random and in agreement with the previously
obtained allozyme and RAPD patterns, although the genetic-diversity values obtained with microsatellites are much higher.
Significant correlates of microsatellite markers with various climatic and soil factors suggest that, as in allozymes and
RAPDs, natural selection causes adaptive microsatellite ecogeographical differentiation, not only in coding, but most importantly
in non-coding genomic regions. Hence, the concept of ”junk DNA” needs to be replaced by at least partly regulatory DNA. The
obtained results suggest that microsatellite markers are useful for the estimation of genetic diversity in natural populations
of T. dicoccoides and for the tagging of agronomically important traits derived from wild emmer wheat.
Received: 27 February 2001 / Accepted: 22 March 2001 相似文献
16.
S. Van Campenhout R. M. D. Koebner G. Volckaert 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(2):328-336
Two wheat consensus primer sets, directed to ”early-methionine-labelled” (Em) gene sequences, were tested for their ability
to amplify beyond their original source. A range of widely diverse templates, including other Triticeae species and sample
monocot and dicot species, was assayed. Primer set EMC5/EMC3, amplifying the entire coding region with its intron and part
of the 3’ untranslated region, targets Triticeae and sorghum Em sequences. The other set, EMC5/EMCO31, directed to the coding
region and its intron, amplifies templates from all the grass species. Both primer sets fail to amplify Em sequences from
more distant monocots and the dicots. Using set EMC5/EMC3, we isolated and sequenced ten members of the rye Em gene family
from five different rye sources. Significant DNA sequence variation between wheat and rye sequences in the non-coding regions
was found, and this was used to develop seven sequence-specific primers. Twelve primer combinations were analysed, 7 of which
were Em-R1-specific, amplifying a product in at least one of the tested rye or rye-carrying genotypes but not in wheat. Four sets exhibited
clear amplification length polymorphisms which allowed discrimination between and within the rye sources. The primers also
discriminated between wheat-rye recombinants with proximal 1RL rye chromatin and those carrying distal 1RL rye chromatin.
These results show that wheat consensus primer sets can be used to isolate orthologous sequences, especially from species
that are used for alien gene transfer in wheat. Subsequently, species-specific assays can be designed that are useful tools
for this application.
Received: 7 December 1998 / Accepted: 21 July 1999 相似文献
17.
P. Rallo G. Dorado A. Martín 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):984-989
We report the development of microsatellites or simple sequence repeats (SSRs) in the olive tree (Olea europaea L.). Forty three positive clones obtained by the screening of a GA-enriched genomic library were sequenced and primers were
designed for 13 microsatellite loci. Five primer pairs amplified polymorphic products of the expected size range. SSR polymorphism
was explored in a set of 46 olive cultivars. A total of 26 alleles were detected for the five loci. Heterozygosity ranged
from 0.46 to 0.71. Ninety one per cent of the cultivars had unique multilocus genotypes. Microsatellite segregation was studied
in a complex population from a cross between the commercial cultivars ’Leccino’ and ’Dolce Agogia’.
Received: 3 February 2000 / Accepted: 21 March 2000 相似文献
18.
The development of a genetic map for meadowfoam comprised of amplified fragment length polymorphisms
Katengam S Crane JM Knapp SJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(1):92-96
Limnanthes alba Benth. (meadowfoam), a diploid (x=5) winter annual, produces novel very long-chain seed oils (C20 and C22) with less than 2% saturated fatty acids. The first genetic map of meadowfoam, a recently domesticated species, is described
herein. Two phenotypically diverse inbred lines, OMF40–11 (L. alba ssp. alba) and OMF64 (L. alba ssp. versicolor), were screened for amplified fragment length polymorphisms (AFLPs) using 16 primer combinations. Twenty three percent of
the AFLP bands (415 out of 1,801) were polymorphic between OMF40–11 and OMF64. One hundred (OMF40–11×OMF64)×OMF64 BC1 progeny were genotyped for 107 polymorphic AFLP markers produced by nine AFLP primer combinations. One hundred and three
AFLP loci amalgamated into five linkage groups with 14 to 28 loci per linkage group (four loci segregated independently).
The map was 698.5-cM long with a mean interlocus spacing of 6.7 cM and no dense clustering of loci. The segregation ratios
for 25 loci (23.2%) were significantly distorted. Twenty one of the distorted loci (84%) had an excess of L. alba ssp. versicolor (recurrent parent) alleles. The distorted loci, apart from one locus on linkage group 4, were distally clustered on both
ends of linkage groups 1, 4 and 5. The development of the map was facilitated by the small chromosome number, an abundance
of restriction site polymorphisms between the two subspecies (23%), and a high multiplex ratio of the AFLP markers (112 per
primer combination).
Received: 16 October 2000 / Accepted: 18 April 2001 相似文献
19.
20.
Y. Mano B. E. Sayed-Tabatabaei A. Graner T. Blake F. Takaiwa S. Oka T. Komatsuda 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):937-946
In order to identify sequence-tagged sites (STSs) appropriate for recombinant inbred lines (RILs) of barley cultivars ‘Azumamugi’ × ‘Kanto
Nakate Gold’, a total of 43 STS primer pairs were generated on the basis of the terminal sequences of barley restriction fragment
length polymorphism (RFLP) clones. Forty one of the 43 primer pairs amplified PCR products in Azumamugi, Kanto Nakate Gold,
or both. Of these, two showed a length polymorphism and two showed the presence or absence of polymorphism between the parents.
PCR products of the remaining 37 primers were digested with 46 restriction endonucleases, and polymorphisms were detected
for 15 primers. A 383.6-cM linkage map of RILs of Azumamugi×Kanto Nakate Gold was constructed from the 19 polymorphic STS
primer pairs (20 loci) developed in this study, 45 previously developed STS primer pairs (47 loci), and two morphological
loci. Linkage analysis and analysis of wheat-barley chromosome addition lines showed that with three exceptions, the chromosome
locations of the STS markers were identical with those of the RFLP markers.
Received: 4 August 1998 / Accepted: 8 October 1998 相似文献