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1.
S. Bergoñón C. Codina J. Bastida F. Viladomat E. Melé 《Plant Cell, Tissue and Organ Culture》1996,45(3):191-199
Galanthamine (GAL) is increasingly used in the treatment of Alzheimer's disease. We have attempted to develop a method of producing this alkaloid using in vitro cultures of Narcissus confusus plants. The “shoot-clump” culture in liquid medium was shown to be an appropriate method for the micropropagation of this bulbous plant. The complete process included three steps:
- culture of “twin-scales” starting from the bulbs;
- culture of the newly formed shoots in a medium for bud proliferation (Murashige Skoog+1 mg l-1 of 2,4-dichlorophenoxyacetic acid+5 mg l-1 of benzyladenine), and
- culture of “shoot-clumps” in a liquid-shake medium. Here we describe the effect of the addition of trans-cinnamic acid, a precursor in the biosynthesis of the Amaryllidaceae alkaloids, on the production of galanthamine and related alkaloids, and also on the growth of the “shoot-clump” culture. The production of galanthamine was found to be inhibited by the addition of the precursor, which promoted the production of the other alkaloid in the same biosynthetic pathway, N-formyl-norgalanthamine. The total production of galanthamine in the control cultures in day-long photoperiod was 2.50 mg per culture, of which 1.97 mg per culture were released into the medium.
2.
Two different morphogenetic pathways, adventitious bud and corm-like structure (CLS), were observed on organogenic calli derived
from the petioles of Amorphophallus albus in vitro. The organogenic calli was established via culture of petiole segments on Murashige and Skoog (MS) medium supplemented
with 1.0 mg l−1 α-naphthaleneacetic acid (NAA) and 1.0 mg l−1 6-benzyladenine (BA) and subculture of the petiole-derived calli on MS medium with 0.5 mg l−1 NAA and 0.5 mg l−1 BA. These organogenic calli were used to induce morphogenesis via culture on MS medium with various concentrations of NAA
and BA. BA alone favoured adventitious bud differentiation (57.0 ± 8.3% at maximum) from the organogenic calli but inhibited
CLS formation. In the presence of NAA and BA, both adventitious bud and CLS were observed in a same culture system. The maximum
CLS formation (71.2 ± 9.3%) were found on MS medium with 0.5 mg l−1 NAA and 2.0 mg l−1 BA, associated with 26.7 ± 8.6% adventitious bud differentiation. A small part of the adventitious buds developed into normal
shoots which needed rooting culture phase to form complete plants. About 80% survival rate was obtained with these plants
after transplantation to soil. More than 90% of the CLSs produced complete plants with shoots and root systems, regardless
of the rooting media tested. Transplantation of the CLS-derived plants to soil gave 100% survival rate. Histological observations
revealed both the two morphogenetic events originated from the meristematic cells located in superficial layers of callus
tissue. 相似文献
3.
Embryogenic cultures were induced from immature avocado zygotic embryos representing different botanical races and complex
hybrids. The optimum induction medium consisted of B5 major salts, MS minor salts, 0.4 mg l−1 thiamine HCl, 100 mg l−1 myo-inositol, 30 g l−1 sucrose, 0.41 μM picloram and 8 g l−1 TC agar. Somatic embryogenesis occurred directly from the explants on induction medium, and secondary embryos and proembryonic
masses proliferated in liquid and on semisolid maintenance medium. Embryogenic culture maintainance was optimized in liquid,
filter-sterilized MS medium, supplemented with 30–50 mg l−1 sucrose, 4 mg l−1 thiamine HCl and 0.41 μM picloram. Two types of embryogenic cultures were recognized: –genotypes that proliferated as proembryonic
masses in the presence of auxin (PEM-type) and; –genotypes in which the heart stage and later stages of somatic embryos developed
in the presence of auxin(SE-type). Embryogenic suspension cultures became increasingly disorganized over time, and this was
associated with progressive loss of embryogenic potential.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
4.
Embryogenic cultures were induced from leaflets from new vegetative flushes of mature ‘Brewster’ litchi trees on B5 medium
containing 400 mg l−1 glutamine, 200 mg l−1 casein hydrolysate, 30 g l−1 sucrose, 4.52 μM 2,4-D, 9.30 μM kinetin and 3 g l−1 gellan gum in darkness. Embryogenic cultures consisting of proembryonic cells and masses were maintained either on semi-solid
MS medium supplemented with 4.52 μM 2,4-D and 0.91 μM zeatin or as embryogenic suspension cultures in liquid medium of the
same composition. Maturation of somatic embryos occurred on semi-solid MS medium with 5–20% (v/v) filter-sterilized coconut
water in darkness. Recovery of plants from somatic embryos was improved with 14.4 μM GA3 on half-strength MS medium with 0.2 g l−1 activated charcoal under a 16 h photoperiod provided by cool white fluorescent lights (60–80 μmol s−1 m−2). Plants have been successfully acclimatized in the greenhouse. 相似文献
5.
A procedure is described to regenerate plants from embryogenic suspension-derived protoplasts of ginger (Zingiber officinale Rosc.). Somatic embryogenic calli were induced from ginger shoot tips on solid MS medium with half the concentration of NH4NO3 and supplemented with 1.0 mg l−1 2,4-Dichloroacetic acid (2, 4-D) and 0.2 mg l−1 Kin. Rapid-growing and well-dispersed suspension cultures were established by subculturing the embryogenic calli in the same
liquid medium. Protoplasts were isolated from embryogenic suspensions with an enzyme solution composed of 4.0 mg l−1 cellulase, 1.0 mg l−1 macerozyme, 0.1 mg l−1 pectolyase, 11% mannitol, 0.5% CaCl2 and 0.1% 2-(N-morpholino) ethane sulphonic acid (MES) for 12–14 h at 27°C with a yield of 6.27 × 106 protoplasts g−1 fresh weight. The protoplasts were cultured initially in liquid MS medium with 1.0 mg l−1 2, 4-D and 0.2 mg l−1 Kin. Then the protoplast-derived calli (1.5 cm2) were transferred to a basal MS medium containing 0.2 mg l−1 2, 4-D, 5.0 mg l−1 benzyladenine (BA), 3% sucrose and 0.7% agar. The white somatic embryos were transferred to MS medium lacking growth regulators
for shoot development. Shoots developed into complete plantlets on a solid MS medium supplemented with 2.0 mg l−1 BA and 0.6 mg l−1 α-Naphthaleneacetic acid (NAA). In addition, the effects of AgNO3, activated charcoal (AC) and ascorbic acid (AA) on browning of protoplast-derived calli are discussed. 相似文献
6.
Effect of genotype,explant type and growth regulators on organogenesis in Morus alba 总被引:1,自引:0,他引:1
Plantlets of the mulberry (Morus alba L. vars. Chinese White, and Kokuso-27) were produced from callus cultures. For callus induction, leaf, internodal segments,
and petiole explants of Chinese White, Kokuso-27 and Ichinose varieties were grown on MS basal medium fortified with 2,4-D
and 6-benzylaminopurine (BA). Callogenesis was dependent on the nature of explant used, the genotype and growth regulators
supplemented in the medium. Leaves were the best explant type used for callus induction. Best callogenesis was obtained on
MS medium containing a combination of 1 mg l−1 2,4-D and 0.5 mg l−1 BA (95-100%). Calluses formed shoots on MS medium supplemented with 1 mg l−1 BA. Supplementation with 0.1 mg l−1 2,3,5-triiodobenzoic acid (TIBA) in this medium enhanced shooting response. Presence of TIBA in the medium also improved
the long-term organogenic potential of the callus. Regenerated shoots produced roots on Murashige & Skoog (MS) medium containing
either 0.5 mg l−1 indole-3-butyric acid (IBA) or α-naphthaleneacetic acid (NAA). Seventy percent of the rooted plants were established in the
field where they are performing well.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
7.
Plant regeneration from Rosa shoot tips cryopreserved by a combined droplet vitrification method 总被引:1,自引:0,他引:1
Shoot tips obtained from in vitro Rosa plants (three cultivars) were successfully cryopreserved by a combined droplet vitrification method and subsequently shoots
regenerated. The excised shoot tips (1–4 mm long) were incubated in a liquid MS medium supplemented with 2.5 mg l−1 thiamine, 0.2 mg l−1 biotin, 0.2 mg l−1 pyridoxine, 0.25 mg l−1 6-benzylaminopurine (BAP), 0.5 mg l−1 gibberellic acid (GA3) and 0.08 M sucrose, for 24 h. Following that incubation shoot tips were pre-cultured in this MS medium containing 0.1 till
1.0 M sucrose for 24 and 48 h, respectively. Pre-cultured shoot tips were dehydrated with concentrated PVS2 cryoprotective
solution for 10–30 min at room temperature, prior to a direct plunge in liquid nitrogen. After rapid rewarming in the above
mentioned liquid medium shoot tips were plated on a modified MS medium (5 g l−1 agar) supplemented with vitamins and plant growth regulators as mentioned above for regrowth. Cryopreserved shoot tips resumed
growth within 10 days and regenerated shoots within 3 weeks. The highest numbers of regrowing shoot tips were 64.44% for cv.
Kardinal, 67.73% for cv. Fairy and 57.57% for cv. Maidy. 相似文献
8.
Yong Duck Kim Ji Yun Min Won Jung Kim Young Min Kang Hyun Shik Moon Cheul Ho Lee D. Theertha Prasad Myung Suk Choi 《In vitro cellular & developmental biology. Plant》2008,44(3):203-208
An efficient in vitro plant regeneration system was established from callus culture of Scopolia parviflora. Callus was induced from adventitious roots on B5 medium with 0.45–9.04 μM 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro plantlet regeneration was achieved on B5 medium supplemented with 44.38 μM benzyladenine (BA), 3% sucrose, and 0.38% gelrite.
Plantlets were transplanted to artificial soil and grown to maturity successfully in a greenhouse. The tropane alkaloid contents
in regenerated plants were analyzed using high-performance liquid chromatography (HPLC), and were found to be higher than
those of adventitious roots, native growing plants, and acclimated plants. Regenerated plants from organogenic callus cultures
produced a greater amount of tropane alkaloids. 相似文献
9.
Abhinav Grover Jayashankar S. Yadav Ranjita Biswas Choppakatla S. S. Pavan Punita Mishra Virendra S. Bisaria Durai Sundar 《Plant Cell, Tissue and Organ Culture》2012,108(2):323-331
Cell suspension cultures of Camellia sinensis were established in 250 ml shake flasks. Flasks contained 50 ml liquid medium of either Murashige and Skoog (MS), N/5 MS
or Heller medium containing different levels of 6-benzyladenine (BA) (0.05–2 mg l−1), 2,4-dichlorophenoxyacetic acid (2,4-D) (1–10 mg l−1), and sucrose (10–50 g l−1). Moreover, the pH of the medium was varied from 5.2–6.2. In addition, cultures were subjected to light irradiation as well
as to complete darkness. Following optimization of aroma and terpenoid extraction methods, cell cultures were analyzed for
the volatile compounds using GC/MS. A total of 43 compounds were identified using the micro SDE apparatus. Among the major
monoterpenoids obtained were α-terpineol and nerol. Moreover, other high aroma-value compounds, including 2-ethyl hexanol,
benzyl alcohol, benzene acetaldehyde, nonanal and phenylethylalcohol were also detected. The highest levels of these compounds
were obtained from cell suspension cultures grown in MS medium containing 5 mg l−1 2,4-D, 1 mg l−1 BA and 30 g l−1 sucrose at pH of 5.8 with incubation in complete darkness. 相似文献
10.
A. V. Raghu Kuzhiyumparambil Unnikrishnan S. P. Geetha Gerald Martin Indira Balachandran 《In vitro cellular & developmental biology. Plant》2011,47(4):506-515
Embelia ribes, an important vulnerable medicinal liana, was regenerated through organogenesis and embryogenesis using leaf explants. Leaf
explants produced organogenic calluses on MS medium supplemented with 1.0 mg l−1 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5 mg l−1 6-benzylaminopurine. Shoot regeneration was obtained from organogenic calluses on MS medium containing different concentrations
of thidiazuron (TDZ) and indole-3-acetic acid (IAA). The frequency of shoot bud organogenesis was highest (23.9 shoots/explant)
in MS medium containing 0.5 mg l−1 TDZ and 0.1 mg l−1 IAA. The best result for induction of embryogenic callus was noticed in the combination of 2.0 mg l−1 TDZ and 0.5 mg l−1 2,4-D. This callus, maintained in the same medium, showed the highest differentiation of embryos (56.5%) after 6 wk of culture.
Embryos were transferred to MS medium supplemented with different concentrations of TDZ, and this facilitated conversion of
embryos into plants. After 6 wk of subculture, MS medium with 0.05 mg l−1 TDZ favored the highest percentage (52.2%) embryo conversion. As per the present protocol, 52.2% of the embryos underwent
conversion, and a mean number of 29.5 shoots per culture was obtained. Shoots developed from both types of calluses were rooted
on half-strength MS basal medium supplemented with 1.0 mg l−1 indole-3-butyric acid. HPLC-UV assay demonstrated the highest embelin content (5.33% w/w) in the embryogenic callus cultures. Embelin was isolated from embryogenic callus and was identified using IR and 1H NMR studies. 相似文献
11.
Jaya Arora Shaily Goyal Kishan Gopal Ramawat 《In vitro cellular & developmental biology. Plant》2010,46(5):430-436
This report demonstrates the elicitation effect on growth and stilbene accumulation in cell cultures of Cayratia trifolia (Vitaceae) by an extract of the angiosperm parasite Cuscuta reflexa and salicylic acid in combination with sucrose feeding. Cell cultures of C. trifolia, a tropical liana, were maintained in liquid Murashige and Skoog's basal medium containing 0.25 mg l−1 naphthalene acetic acid, 0.2 mg l−1 kinetin with 3% sucrose and 250 mg l−1 casein hydrolysate. The cells treated with Cuscuta elicitor showed increased polyphenol oxidase activity with increasing concentration of the elicitor, while total phenol content
remained almost unchanged. Enhanced yield of stilbenes (∼8-fold) was recorded in the cells treated with 200 mg l−1
Cuscuta elicitor for 7 d. Optimum accumulation of stilbenes with a non-significant decrease in cell growth as compared with control
was recorded with the addition of 3% sucrose on the seventh day of cell culture. Addition of 3% sucrose with salicylic acid
at 500 μM and Cuscuta extract at 200 mg l−1 on the seventh day enhanced total stilbene yield up to 50.1 mg l−1, which was ∼14-fold higher than in control cultures. Piceid content increased ∼200-fold in such cultures. 相似文献
12.
Nokwanda P. Makunga Anna K. Jäger Johannes van Staden 《Plant Cell, Tissue and Organ Culture》2006,86(1):77-86
Stock cultures of Thapsia garganica grown on Murashige and Skoog agar medium (1962) (MS) (0.8% agar [w/v]; pH 5.8) with 0.5 mg l−1 NAA and 1.5 mg l−1 BA were best rooted by subjecting to half strength MS liquid medium with IBA (10 mg l−1) for 3 days prior to transfer to medium without plant growth regulators. A rooting frequency of 60% was noted with seven roots per rooted plant. Rooting was apparent after 10 days. The present study also aimed at reducing the occurrence of hyperhydric plants. The inclusion of 2% polyethylene glycol (w/v) in the growth medium or ventilation of cultures prior to acclimatization resulted in the production of plants true to type, closely resembling wild plants. Those plantlets that had been rooted and exposed to a better vented environment were able to acclimatize readily. Tissue culture propagation is therefore beneficial to the conservation of the medicinally important species, Thapsia garganica. 相似文献
13.
Sun Hee Woo Arun Nair Taiji Adachi Clayton G. Campbell 《In vitro cellular & developmental biology. Plant》2000,36(5):358-361
Summary Plants were regenerated from cotyledon tissue of greenhouse grown seedlings of common buckwheat (Fagopyrum esculentum Moench.). Maximum callus regeneration was induced on Murashige and Skoog (MS) medium containing 2,4-D (2.0 mg l−1) and kinetin (KIN) (0.2 mg l−1) and either 3 or 6% sucrose. Friable callus was transferred to MS media containing KIN and benzylaminopurine (BAP) at varied
concentrations for embryogenic callus induction. The optimum medium for embryogenic callus induction was found to be MS medium
supplemented with 0.2 mg l−1 KIN, 2.0 mg l−1 BAP and 3% (w/v) sucrose. Variation of sucrose from 3 to 6% did not show any significant effect on callus induction or embryogenesis.
Regeneration of embryonic callus varied from 13 to 32%. Whole plants were obtained at high frequencies when the embryogenic
calluses with somatic embryos and organized shoot primordia were transferred to half-strength MS media with 3% sucrose. Regenerated
plants after acclimation were transferred to greenhouse conditions, and both vegetative and floral characteristics were observed
for variation. This regeneration system may be valuable for genetic transformation and cell selection in common buckwheat. 相似文献
14.
Simões-Gurgel Claudia Cordeiro Lívia da Silva de Castro Tatiana Carvalho Callado Cátia Henriques Albarello Norma Mansur Elisabeth 《Plant Cell, Tissue and Organ Culture》2011,106(3):537-545
The effects of different levels of Murashige and Skoog (MS) basal medium, 2,4-dichlorophenoxyacetic acid (2,4-D), and sucrose
on anthocyanin production and biomass accumulation of cell suspension cultures of Cleome rosea were investigated. Cultures were established in liquid MS medium containing 30 g l−1 sucrose and supplemented with 0.90 μM 2,4-D. Proliferating cell suspension cultures achieved the highest growth capacity,
a fourfold increase in biomass accumulation, following subculture at the exponential growth phase, 14–18 days of culture.
Moreover, the presence of 2,4-D was essential for anthocyanin production and biomass accumulation. On the other hand, increasing
levels of sucrose above 30 g l−1 resulted in a drastic reduction in biomass accumulation. Anthocyanin production was highest in cell suspension cultures grown
on half-strength MS medium (1/2 MS), 30 g l−1 sucrose, and 0.45 μM 2,4-D. These cell suspension cultures were mainly composed of small aggregates of spherical cells with
similar morphology observed in anthocyanin-producing and non-producing cultures. Moreover, microscopic analysis of anthocyanin-producing
cultures showed the presence of mixtures of non-pigmented, low-pigmented, and high-pigmented cells. 相似文献
15.
Boubacar Dary Sima Yves Desjardins Le Van Quy 《In vitro cellular & developmental biology. Plant》2001,37(4):480-489
Summary The effect of sucrose on in vitro potato (ev. Kennebec) metabolism was evaluated. Plants were grown in three different media: Murashige and Skoog basal medium
containing high nitrogen concentration with 0 or 20 g l−1 sucrose; or modified medium containing reduced nitrogen amount and 20 g l−1 sucrose. Plants fed with 20 g l−1 sucrose and high N exhibited higher phosphoenolpyruvate carboxylase (PEPC) and pyruvate kinase activities and high PEPC protein
concentration at 7, 20 and 33 d of culture compared to those grown with 20 g l−1 sucrose and low N, or with 0 g l−1 sucrose and high nitrogen (control). The highest accumulation of starch and sucrose was found in plants grown with sucrose
and low nitrogen. This accumulation occurred concomitantly with a reduced enzyme activity resulting from a low utilization
of α-ketoglutarate by nitrogen assimilation, when plants were grown with reduced nitrogen. Our investigations on tricarboxylic
acid cycle activity showed that sucrose led to the reduction of organic acid amounts in both leaves and roots when high nitrogen
was supplied to plants. This was probably due to the intense exit of α-ketoglutarate, which was confirmed by measurements
of cytosolic isocitrate dehydrogenase activity. The low leaf glutamine/glutamate ratio observed in plants grown with 20 g
l−1 sucrose and high nitrogen compared to their counterparts cultivated with low nitrogen might be due to glutamine conversion
into proteins when nitrogen assimilation was intense. These results demonstrate that sucrose enhanced PEPC activity by increasing
protein synthesis. They also suggest that sucrose metabolism is involved in the replenishment of the tricarboxylic acid cycle
by providing carbon skeletons required to sustain phosphoenolpyruvate utilization during high nitrate assimilation. 相似文献
16.
Protoplasts were isolated from cell suspensions derived from cotyledon and hypocotyl Gentiana kurroo (Royle). Cell walls were digested with an enzyme cocktail containing cellulase, macerozyme, driselase, hemicellulase and
pectolyase in CPW solution. Protoplast viability ranged from 88 to 96%. Three techniques of culture and six media were evaluated
in terms of their efficiency in producing viable cultures and regenerating whole plants. With liquid culture, cell division
occurred in only a low number of the protoplasts isolated, and no plant regeneration was successful. Cell division occurred
within 2 or 3 days in case of agarose solidified media. After 10 days of culture, the number of dividing cells was the highest
with modified MS medium in which NH4NO3 was replaced with 3.0 g l−1 glutamine. The best results were obtained with agarose bead cultures: plating efficiency was 68.7% and 58.1% for protoplasts
isolated from cotyledon and hypocotyl derived suspensions, respectively. The results were achieved with using medium containing
0.5 mg l−1 2,4-D + 1.0 mg l−1 kinetin or 2.0 mg l−1 BAP + 1.0 mg l−1 dicamba + 0.1 mg l−1 NAA + 80 mg l−1 adenine sulfate. Protocalluses transferred on the following composition of plant growth regulators: 0.5 mg l−1 2,4-D + 1.0 mg l−1 kinetin or 1.0 mg l−1 kinetin + 0.5 mg l−1 GA3 + 80.0 mg l−1 adenine sulfate developed in embryogenic cultures. However, the best embryo production occurred with the first one. Later
embryos were transferred to half-strength MS mineral salts to promote plants formation. Flow cytometry studies revealed increased
amounts of DNA in about one third of the regenerants. 相似文献
17.
B. Duquenne T. Eeckhaut S. Werbrouck J. Van Huylenbroeck 《Plant Cell, Tissue and Organ Culture》2006,87(3):329-331
A method for regeneration of plants from tuber explants of a Zantedeschia hybrid via somatic embryogenesis was developed. In vitro cultures were initiated starting from both anthers and tubers. Somatic embryogenesis was only achieved from tuber explants. 6-Benzyladenine (BA) at 0.6 or 2 mg l−1 in combination with 2 mg l−1 α-naphthaleneacetic acid (NAA) yielded the highest number of embryos per explant. The somatic embryos converted into plantlets on Murashige and Skoog basal medium supplemented with vitamins, micro- and macronutrients, 1 mg l−1 6-τ-τ-(dimethylallylamino)-purine (2iP), 3% sucrose and 0.7% agar. This is the first report on induction of somatic embryogenesis in Zantedeschia. 相似文献
18.
De Klerk Geert-Jan Brugge Jolanda Ter Marinova Svetla 《Plant Cell, Tissue and Organ Culture》1997,50(1):39-44
The production of an antifungal spirostanol saponin designated SC-1 has been detected in cell suspension cultures of the Mexican
species Solanum chrysotrichum. Batch cultures of a cell suspension obtained from hypocotyl derived calluses of this species
were grown for 25 days in shake flasks containing Murashige & Skoog (MS) medium. Throughout the growth cycle, fresh and dry
weight, SC-1 yield, and uptake of sucrose, glucose and fructose were determined. The effects of inoculum size and sucrose
concentration on the biomass accumulation and synthesis of the active metabolite, were studied. The maximum SC-1 production,
above 14 mg.g−1 (which was fifty times that of field grown plants), was reached after 20 days using a 2% inoculum and complete MS medium
supplemented with 2 mgl−1 2,4-D, 2 mg l−1kinetin, and sucrose between 30 and 45 gl−1.
.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
19.
Su Wei Wen Hwang Wen-Ing Kim Se Young Sagawa Yoneo 《Plant Cell, Tissue and Organ Culture》1997,50(2):91-95
A modified culture protocol has been developed for the induction of somatic embryogenesis in Azadirachta indica (neem). Embryogenic calluses were initiated from cotyledons or hypocotyls using a Murashige and Skoog (MS) agar medium supplemented
with 0.5 mg l−1 α-napthaleneacetic acid (NAA), 1 mg l−1 6-benzylaminopurine (BA), 1 g l−1 casein hydrolysate, and 50 g l−1 sucrose. The calluses, when transferred to a liquid medium similar to the agar medium but with NAA replaced by 0.5 mg l−1 indole-3-acetic acid (IAA), formed globular structures which further developed a rudimentary root, after 4 to 5 weeks incubation.
Subsequently, these highly differentiated tissues when transferred into a hormone-free MS medium containing 1 g l−1 casein hydrolysate and 50 g l−1 sucrose, active embryo masses started to appear after 1 to 2 weeks. The embryo production was found to improve more than
2 fold by adding 0.2 mg l−1 zeatin to the medium.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
20.
The Root cultures of Cayratia trifolia (Vitaceae) a tropical lianas, were maintained in liquid Murashige and Skoog’s medium containing 0.5 mg l−1 NAA, 0.1 mg l−1 kinetin with 3% sucrose. These root cultures when grown with 6% sucrose accumulated stilbenes (piceid, resveratrol, viniferin,
ampelopsin) in high amounts, which on elicitation by 500 mg l−1 yeast extract, 50 μM salicylic acid (SA), 50 μM methyl jasmonate (MeJa), 500 μM ethrel added at 25th day, increased up to
ninefolds (7.1 mg l−1). Addition of alar or phenylalanine along with the elicitors further enhanced the stilbenes content. In the present study,
stilbenes accumulation up to 12 folds (9.2 mg l−1) was obtained with SA and alar. The SA was the most effective in increasing the stilbenes contents while less than control
values were recorded in the cells treated with MeJa. The roots could be grown up to 2 l flasks. The present work demonstrates
that presence of precursor and sucrose during elicitation at an appropriate time combined with growth retardation significantly
increased the production of stilbenes in C. trifolia cell cultures. 相似文献