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1.
水稻DH群体的分子连锁图谱及基因组分析 总被引:13,自引:0,他引:13
利用扩大的籼粳杂交来源(窄叶青8号×京系17)的水稻(OryzasativaL.)加倍单倍体(DH)群体,构建了包含444个位点的分子连锁图谱,覆盖水稻基因组1962cM(centiMorgon),标记间的平均图距小于5cM。此图谱包括276个RFLP标记、34个RAPD标记、89个微卫星标记、10个AFLP标记、26个端粒重复相关序列(TAS)标记以及9个同工酶标记。该遗传图谱与其它的水稻高密度遗传图谱具有较高的可比性,并有自己的特点,适于进行各种持续性的遗传学研究 相似文献
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RAPD标记构建家蚕分子链锁图 总被引:2,自引:1,他引:1
以大造、C108及其F2群体构建了1个家蚕的RAPD连锁框架图,该图含RAPD标记位点182个,来自大造的103个标记分属前23个连锁群,来自C108的79个标记分属后16个连锁群,覆盖基因组的总长度为1148.3cM(centimorgan),它能与本室用同一群体构建的SADF图谱相整合,亦可与相应的RFLP图谱相互补充。 相似文献
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中国白菜RAPD分子遗传图谱的构建 总被引:17,自引:0,他引:17
以芜菁(Brassica campestris L.ssp.rapifera Metzg)和结球白菜(B.carnpestris L.ssp .Plkinensis(Lour.)Oisson)杂交的F2群体为试材,采用RAPD标记,用84个10核苷酸随机引物构建了白菜的RAPD遗传图谱。该图谱覆盖基因组的1632.4cM(certi Morgan)标记间的平均间隔为16.5cM。其中最长的连锁群为 相似文献
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与西瓜野生种质抗枯萎病基因连锁的RAPD标记 总被引:23,自引:0,他引:23
运用RAPD技术,采用混合分组分析(bulkedsegregantanalysis,BSA)方法进行了西瓜(Citrulluslanatus(Thunb.)Mansfeldvar. citroides) 野生种质PI296341 抗枯萎病基因连锁的分子标记研究。研究结果表明:西瓜野生种质P1296341 抗枯萎病生理小种1 的抗性由单显性基因控制,RAPD标记OPPOL/700 与其抗病基因连锁,其遗传距离为30 cM(centimorgan)。这为进行抗病分子标记辅助选择,以及最终定位与克隆其抗病基因打下了良好基础。 相似文献
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利用分子标记定位农垦58S的光敏核不育基因 总被引:17,自引:0,他引:17
对农垦58S(Oryzasativasp.japonica)/大黑矮生标记基因系FL2组合组建可育集团和不育集团,并以亲本为对照进行了RFLP、RAPD和双引物RAPD分析,结果第12染色体上的一个单拷贝标记G2140与光敏核不育基因连锁遗传,二者间的遗传图距为14.1cM(centimorgan)。在筛选过的1040个随机单引物和190个双引物中,仅引物OPAU10扩增出与光敏核不育基因连锁的1.5kbDNA片段,回收、克隆该DNA片段并制备探针,将其转换成共显性的RFLP标记并命名为OPAU101500。分离群体连锁分析表明该标记与标记G2140紧密连锁,将农垦58S的一对光敏核不育基因定位于第12染色体上。 相似文献
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烟草黑胫病菌株亲缘关系的RAPD分析 总被引:15,自引:1,他引:14
从220个RAPD(Random Amplified Polymorphic DNAs)随机引物中所选出的多态扩增性强的21个引物对来源不同的33个烟草黑胫病菌株进行全基因组DNA遗传多样性分析和指纹构建。选用引物对受试菌株进行RAPD-PCR扩增,共产生243条DNA标记图带,其中191条为多态性图带,多态检测率为78.6%。利用UPGMA(Unweigthted Pair-group Meth 相似文献
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显微分离出黑麦(SecalecerealeL.)1R染色体,用CohesiveadapterssingleprimerPCR(CASPPCR)方法进行体外扩增,以DIG11dUTP标记扩增产物为探针,进行Southern分子杂交,结果表明扩增产物来自黑麦1R染色体。用1/10体积的连接物转化E.coliDH5α,获得10000多个重组菌落。经酶切分析,克隆子的插入片段为250~500bp,为进一步筛选1R染色体的分子标记打下了基础 相似文献
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Genetic maps provide an important genomic resource of basic and applied significance. Spruce (Picea) has a very large genome size (between 0.85 × 1010 and 2.4 × 1010 bp; 8.5-24.0 pg/1C, a mean of 17.7 pg/1C ). We have constructed a near-saturated genetic linkage map for an interspecific backcross (BC1) hybrid of black spruce (BS; Picea mariana (Mill.) B.S.P.) and red spruce (RS; Picea rubens Sarg.), using selectively amplified microsatellite polymorphic loci (SAMPL) markers. A total of 2284 SAMPL markers were resolved using 31 SAMPL-MseI selective nucleotide primer combinations. Of these, 1216 SAMPL markers showing Mendelian segregation were mapped, whereas 1068 (46.8%) SAMPL fragments showed segregation distortion at α = 0.05. Maternal, paternal, and consensus maps consistently coalesced into 12 linkage groups, corresponding to the haploid chromosome number (1n = 1x = 12) of 12 in the genus Picea. The maternal BS map consisted of 814 markers distributed over 12 linkage groups, covering 1670 cM, with a mean map distance of 2.1 cM between adjacent markers. The paternal BS × RS map consisted of 773 markers distributed over 12 linkage groups, covering 1563 cM, with a mean map distance of 2.0 cM between adjacent markers. The consensus interspecific hybrid BC1 map consisted of 1216 markers distributed over 12 linkage groups, covering 1865 cM (98% genome coverage), with a mean map distance of 1.5 cM between adjacent markers. The genetic map reported here provides an important genomic resource in Picea, Pinaceae, and conifers. 相似文献
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A rice (Oryza sativa L. ) molecular linkage map has been constructed form over 52 random amplified polymorphic DNA (RAPD) markers with the double haploid (DH) population. It covered a total genetic distance of over 898.4 cM (centimorgan) with 17.3 cM between markers and was complemental with RFLP linkage map. 相似文献
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Sicard D Legg E Brown S Babu NK Ochoa O Sudarshana P Michelmore RW 《Fungal genetics and biology : FG & B》2003,39(1):16-30
The genetic map of Bremia lactucae was expanded utilizing 97 F(1) progeny derived from a cross between Finnish and Californian isolates (SF5xC82P24). Genetic maps were constructed for each parent utilizing 7 avirulence genes, 83 RFLP markers, and 347 AFLP markers, and a consensus map was constructed from the complete data set. The framework map for SF5 contained 24 linkage groups distributed over 835cM; the map for C82P24 contained 21 linkage groups distributed over 606cM. The consensus map contained 12 linkage groups with markers from both parents and 24 parent-specific groups. Six avirulence genes mapped to different linkage groups; four were located at the ends of linkage groups. The closest linkages between molecular markers and avirulence genes were 3cM to Avr4 and 1cM to Avr7. Mating type seemed to be determined by a single locus, where the heterozygote determined the B(2) type and the homozygous recessive genotype determined the B(1) type. 相似文献
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Genetic markers (microsatellites and SNPs) were used to create and compare maps of the turkey and chicken genomes. A physical map of the chicken genome was built by comparing sequences of turkey markers with the chicken whole-genome sequence by BLAST analysis. A genetic linkage map of the turkey genome (Meleagris gallopavo) was developed by segregation analysis of genetic markers within the University of Minnesota/Nicholas Turkey Breeding Farms (UMN/NTBF) resource population. This linkage map of the turkey genome includes 314 loci arranged into 29 linkage groups. An additional 40 markers are tentatively placed within linkage groups based on two-point LOD scores and 16 markers remain unlinked. Total map distance contained within linkage groups is 2,011 cM with the longest linkage group (47 loci) measuring 413.3 cM. Average marker interval over the 29 linkage groups was 6.4 cM. All but one turkey linkage group could be aligned with the physical map of the chicken genome. The present genetic map of the turkey provides a comparative framework for future genomic studies. 相似文献
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利用RAPD标记和单株树大配子体构建马尾松的分子标记连锁图谱 总被引:39,自引:0,他引:39
利用300个随机的寡核苷酸引物和马尾松(Pinus massoniana Lamb.)种子的胚乳(大配子体)产生的随机扩增多态性DNA(RAPD)分子标记,进行马尾松标记连锁图谱的构建。经筛选共获得64个稳定的RAPD标记,利用多点连锁分析,其中的48个标记分属于13个不同的连锁群(连锁对),该图谱覆盖的基因组总长度约为692.5cM(centimorgan),标记间平均距离约为14.7cM,它为马尾松连锁图谱的构建提供了一个连锁框架。 相似文献
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A set of 300 random ohgonucleotide primers was screened for random amplified polymorphic DNA (RAPD) fragments within a sample of 79 megagametophyte DNAs of a single masson pine ( Pinus massoniana Lamb. ) to generate RAPD markers and construct the molecular linkage map for masson pine. 64 repeatable RAPD markers segregated in a 1 present to 1 absent ratio. Through multipoint linkage analysis, 47 markers were assigned to 13 different linkage groups(pairs). It covered a tdtal genetic distance of over 692.5 cM (centimorgan). The average distance between markers was 14.7 cM. It supplied a linkage framework for a more saturated linkage map of masson pine. 相似文献
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Amplified fragment length polymorphisms (AFLP) are dominant markers frequently used to build linkage maps where heterozygosity could be inferred by a backcross breeding strategy. In the present study, we describe the utilization of an unmanipulated great reed warbler, Acrocephalus arundinaceus pedigree to infer heterozygous genotypes of AFLP markers in order to map these markers to a partial linkage map previously based on microsatellites. In total, 50 of the 83 autosomal AFLPs (60%) and 4 of 5 Z-linked AFLPs (80%) were mapped. For each marker, on average, 88% of the expected number of heterozygote parents was detected. The likelihood of map assignment was to a large extent due to the number and density of microsatellite markers already in the map. The 'parsimonious linkage map', that is the map based on the most parsimonious location of all significantly linked markers, consisted of 21 autosomal linkage groups with 2 to 15 markers and had a total map size of 552 cM in males and 858 cM in females. The Z-chromosome linkage group with 12 markers had a size of 155 cM. The autosomal 'framework linkage map', that is the map based only on markers with an unambiguous position, had a total size of 237 cM in males and 440 cM in females, respectively. The inclusion of AFLPs enlarged the previous map substantially (e.g. the autosomal parsimonious linkage map became 441 cM and 621 cM larger for male and female recombination, respectively). The probability that an AFLP became mapped increased with increasing level of heterozygosity, whereas the probability of mapping into a framework position increased with both heterozygosity and number of genotyped individuals. Our results suggest that AFLP provides a fast and inexpensive means of enlarging genetic maps already composed of markers with high polymorphism, also in wild populations with unmanipulated pedigrees. 相似文献
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A high density RFLP linkage map of sugar beet. 总被引:4,自引:0,他引:4
C Halldén A Hjerdin I M Rading B Fridlundh G Johannisdottir S Tuvesson C Akesson T S?ll N O Nilsson 《Génome》1996,39(4):634-645
A high density sugar beet RFLP map with an average distance of 1.5 cM between markers has been constructed. The map covers 621 cM and includes 413 markers distributed over the nine linkage groups of sugar beet. The map is based on two F2 populations representing two different pairs of parents. The two sets of data were integrated into a single map using 90 markers that were common to both data sets. The quality of the map was assessed in several ways. The common markers were used to investigate how often the loci had been mapped in the same order in the two F2 populations. For closely situated markers (<1.5 cM) the order specified in the map is uncertain, but for markers separated by more than 2 cM the locus order is highly reliable. The error rate of the overall process was estimated at 0.3% by independently repeating the analysis of 41 markers. The map is comparatively short, with a map length corresponding to approximately 1.4 crossovers per bivalent. Another feature of the map is a high degree of clustering of markers along the linkage groups. With the possible exception of linkage group 2, each linkage group shows one major cluster, which in most cases is situated in the centre of the linkage group. Our interpretation is that sugar beet, in comparison with most other species, has an extreme localization of recombination. Key words : sugar beet, linkage, RFLP, clustering. 相似文献
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In this study, the first genetic map of Luohanguo (Siraitia grosvenorii (Swingle) C. Jeffrey) was constructed with 150 F? population individuals using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers. A total of 100 ISSRs and 196 SRAP primer combinations generated 51 and 222 polymorphic markers, respectively. Among the 273 markers obtained, 199 markers (29 ISSRs and 170 SRAPs) were mapped to 25 linkage groups. The map covered 1463.3 cM with a mean map distance of 7.35 cM between adjacent markers and a maximum map distance of 52.6 cM between two markers. The markers were distributed randomly in 25 groups except for minor clusters in the distal region of linkage groups. All 25 linkage groups consisted of 2-36 loci ranging in length from 19.5 to 152.6 cM and accounted for 59.8% of the total map distance. This map provides reference information for future molecular breeding work on Luohanguo. 相似文献
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A. M. Risterucci L. Grivet J. A. K. N’Goran I. Pieretti M. H. Flament C. Lanaud 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):948-955
The first linkage map established by Lanaud et al. (1995) was used as a starting point to produce a high-density molecular
linkage map. A mapping population of 181 progenies resulting from a cross between two heterozygous genotypes, a Forastero
and a Trinitario (hybrid between Forastero and Criollo), was used for the linkage analysis. A new DNA isolation protocol was
established, which allows enough good quality DNA to construct a genetic map with PCR-based markers. The map comprises 424
markers with an average spacing between markers of 2.1 cM. The marker types used were five isozymes, six loci from known function
genes, 65 genomic RFLPs, 104 cDNA RFLPs, three telomeric probes, 30 RAPDs, 191 AFLPs and 20 microsatellites. The use of new
marker types, AFLP and microsatellites, did not disturb the original order of the RFLP loci used on the previous map. The
genetic markers were distributed over ten linkage groups and cover 885.4 cM. The maximum distance observed between adjacent
markers was 16.2 cM, and 9.4% of all loci showed skewed segregation.
Received: 2 January 2000 / Accepted: 12 February 2000 相似文献