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人源抗严重急性呼吸综合征(SARS)病毒基因工程抗体的初步研究 总被引:5,自引:0,他引:5
严重急性呼吸道综合征(severe acute respiratory syndrome,SARS)或称传染性非典型肺炎,已严重威胁人民健康和生命安全。快速研制一种可用于紧急预防SARS病毒感染的基因工程抗体预防制剂迫在眉睫。为此,运用噬菌体表面呈现技术,从多个SARS病人恢复期血中获得淋巴细胞,通过基因工程手段,构建了人源抗SARS病毒基因工程抗体文库,并筛选获得37株特异抗SARS病毒基因工程Fab抗体,其中ll株人源抗体结合基因工程重组的SARS病毒核(N)蛋白,其中的1株在Western blot分析中与SARS病毒结合,识别SARS病毒N蛋白线性位点。对所获抗体的功能鉴定及基因分析正在进行中。人源抗SARS病毒基因工程抗体的获得,将对SARS疾病的特异性预防,治疗和诊断提供新的途径。 相似文献
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重组SARS病毒N蛋白可与SARS患者血清发生特异反应 总被引:1,自引:0,他引:1
N蛋白是SARS CoV病毒基因组编码病毒核衣壳蛋白 ,它不同于现已知的任何蛋白质 .对它的深入研究对揭示SARS -CoV的致病机理和疫苗及诊断试剂的研制有重要意义 .灭活的病毒经逆转录后 ,用根据已知的病毒的基因组序列所设计的引物PCR扩增N蛋白基因 .扩增出的基因经序列分析表明和已知的序列完全一致 ,共编码 4 2 2个氨基酸残基 .将N蛋白基因克隆入原核表达载体pET2 8a构建成表达质粒pET2 8a N .表达质粒转化大肠杆菌BL2 1 (DE3) ,并用IPTG诱导后 ,获得了高表达N蛋白的重组菌株 .目的蛋白经一步金属离子螯合层析纯化后获得了纯度超过 90 %的样品 .Western印迹及ELISA分析表明 ,SARS患者体内有特异性的针对N蛋白的抗体 ,并具有较高的特异性 .这为临床上诊断SARS患者提供了新方法 ,并为SARS疫苗的研制提供了研究思路 相似文献
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Bermingham A Heinen P Iturriza-Gómara M Gray J Appleton H Zambon MC 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2004,359(1447):1083-1089
The emergence of new viral infections of man requires the development of robust diagnostic tests that can be applied in the differential diagnosis of acute illness, or to determine past exposure, so as to establish the true burden of disease. Since the recognition in April 2003 of the severe acute respiratory syndrome coronavirus (SARS-CoV) as the causative agent of severe acute respiratory syndrome (SARS), enormous efforts have been applied to develop molecular and serological tests for SARS which can assist rapid detection of cases, accurate diagnosis of illness and the application of control measures. International progress in the laboratory diagnosis of SARS-CoV infection during acute illness has led to internationally agreed World Health Organization criteria for the confirmation of SARS. Developments in the dissection of the human immune response to SARS indicate that serological tests on convalescent sera are essential to confirm SARS infection, given the sub-optimal predictive value of molecular detection tests performed during acute SARS illness. 相似文献
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KONG Qi QIN Chuan Department of Pathology Institute of Laboratory Animal Sciences CAMS&PUMC Beijing 《中国实验动物学报》2005,(Z1)
SARS Research in China 相似文献
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严重急性呼吸综合征(SARS)冠状病毒抗体的检测及其临床意义 总被引:1,自引:0,他引:1
To investigate the k inetics of antibody to SARS coronavirus in SARS patients and its clinical implication,ELISA was used to dete ct antibody to SARS coronavirus(SA RS CoV),RT-PCR was used to detect the SARS CoV RNA and,besides,the C D+4 and CD+8 T cells in peripheral blood of SARS patients and healthy controls were assayed by flowcytom etryThe results showed that SARS CoV IgM were first detected from da y 7 to day 47 after SARS onset,wit h average at day 193±101SARS Co V IgG were first detected from day 4 to day 47 after SARS onset,with average at day 207±101,and the p roduction of SARS CoV IgG was corr elated with CD+4 T cell number(P<005),but had no relationship with SARS CoV RNAMost SARS patients pr oduced SARS CoV antibody,IgM produ ced almost at the same time wit h IgGSARS CoV IgG is a protective antibody against SARS CoV and the titer of IgG may be used as an ind ex indicating the specific immunit y production in SARS patients 相似文献
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严重急性呼吸综合征(SARS)患者不同组织中冠状病毒的分离和鉴定 总被引:4,自引:0,他引:4
严重急性呼吸综合征(SARS)自2002年11月在中国广东爆发后,已迅速蔓延成为全球性传染疾患。为了了解SARS冠状病毒的特征,对先前SARS冠状病毒PCR检测呈阳性的来自广东的3份尸检肺组织标本、2份尸检脾组织标本:来自北京的2份咽拭子标本和1份血清标本,利用10种不同的细胞系分离病毒。结果显示,上述标本在感染细胞后,分别可在293、Vero—E6、Vero、RD和HeLa细胞系中产生细胞病变(CPE)。不同标本在上述细胞系中致CPE的能力不同,但CPE出现的时间和病变形态学特征无显著性差异。以恢复期SARS病人血清为抗体,用间接免疫荧光法对感染后细胞培养的检测,冠状病毒RT-_PCR对感染后细胞RNA的检测,初步证明分离的病毒为冠状病毒。结果再次证明冠状病毒为SARS的病原,它具有较广泛的器官分布和细胞感染能力。血清中SARS冠状病毒的分离,高度提示在SARS发病过程中存在有病毒血症。 相似文献
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Garman E 《Structure (London, England : 1993)》2005,13(11):1582-1583
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Mizutani T 《Uirusu》2004,54(1):97-105
The serious respiratory disease, SARS (Severe Acute Respiratory Syndrome), outbreaking in winter of 2003 to 2004 remained in a sporadic patient's generating at this winter. However, there is also a possibility that wild animals as the source of infection may not be specified and that it may be much in fashion again. The paper regarding SARS and SARS-CoV is published at one per day now which has passed since fashion of SARS in one or so year. There are many papers which the researchers of other viruses enter into the research field of SARS-CoV using their own technology in addition to the researchers of coronavirus. Topics of the research on the present SARS-research field are development of vaccine, inspecting of medicine and establishment of diagnostic method. Here, the newest information is offered about these researches. 相似文献
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SARS流行病传染动力学研究 总被引:5,自引:0,他引:5
Logistic确定型增长模型可被用来描述严重急性呼吸道综合症(SARS)的流行规律,通过对部分国家、地区及中国内地部分省市的数据进行拟合,及其对拟合结果的分析,揭示了各个地区SARS传染力不均匀的现象,以及在控制措施上的差异所带来的不同效果.同时,还对超级传播现象(SSEs)等问题进行了讨论. 相似文献
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Stepping acquired immunodeficiency syndrome (AIDS), severe acute respiratory syndrome (SARS) as another type of disease has been threatening mankind since late last year. Many scientists worldwide are making great efforts to study the etiology of this disease with different approaches. 13 species of SARS virus have been sequenced. However, most people still largely rely on the traditional methods with some disadvantages. In this work, we used Wolfram approach to study the relationship among SARS viruses and between SARS viruses and other types of viruses, the effect of variations on the whole genome and the advantages in the analysis of SARS based on this novel approach. As a result, the similarities between SARS viruses and other coronavirusxes are not really higher than those between SARS viruses and non-coronaviruses. 相似文献
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Tseng CT Sbrana E Iwata-Yoshikawa N Newman PC Garron T Atmar RL Peters CJ Couch RB 《PloS one》2012,7(4):e35421