GENETIC ARCHITECTURE OF ISOLATION BETWEEN TWO SPECIES OF SILENE WITH SEX CHROMOSOMES AND HALDANE'S RULE

Examination of the genetic architecture of hybrid breakdown can provide insight into the genetic mechanisms of commonly observed isolating phenomena such as Haldane's rule. We used line‐cross analysis to dissect the genetic architecture of divergence between two plant species that exhibit Haldane's rule for male sterility and rarity, Silene latifolia and Silene diclinis. We made 15 types of crosses, including reciprocal F₁, F₂, backcrosses, and later‐generation crosses, grew the seeds to flowering, and measured the number of viable ovules, proportion of viable pollen, and sex ratio. Typically, Haldane's rule for male rarity in XY animal hybrids is explained by interactions involving recessive X‐linked alleles that are deleterious when hemizygous (dominance theory), whereas sterility is explained by rapid evolution of spermatogenesis genes (faster‐male evolution). In contrast, we found that the genetic mechanisms underlying Haldane's rule between the two Silene species did not follow these conventions. Dominance theory was sufficient to explain male sterility, but male rarity likely involved faster‐male evolution. We also found an effect of the neo‐sex chromosomes of S. diclinis on the extreme rarity of some hybrid males. Our findings suggest that the genetic architecture of Haldane's rule in dioecious plants may differ from those commonly found in animals.     

HERITABILITY AND SELECTION ON TARSUS LENGTH IN THE PIED FLYCATCHER (FICEDULA HYPOLEUCA)

The heritability estimate (±SE) for tarsus length in the pied flycatcher is 0.53 ± 0.10, based on mother-offspring regressions. The heritability is almost the same (0.50 ± 0.22) for offspring transferred to other nests and reared by foster parents, whereas there is no resemblance (0.04 ± 0.23) between the offspring and their foster mothers. Hence, the nest environment does not affect parent-offspring resemblance. However, a full-sib correlation yields an estimate of the heritability twice as high as the parent-offspring regression did, indicating that shared environment effects, which are not correlated with the tarsus length of parents, must be important. An environmental deviation due to food factors affecting tarsus length is demonstrated. The most important food factors are probably associated with 1) polygyny, which leads to reduced paternal feeding at secondary nests, 2) high breeding density, and 3) progress of the breeding season. All three are associated with reductions in offspring mean tarsus length. We estimate selection on tarsus length for the major components of lifetime reproductive success. Offspring with the shortest tarsi have reduced survival from fledging until breeding, and males with tarsus length close to the mean are most successful in attracting mates. Clutch size increases with female tarsus length, except for individuals with very long tarsi. In general, directional selection is weak, but stabilizing selection is rather strong for survival and male mating success.     

ECOLOGICAL BARRIERS TO GENE FLOW BETWEEN RIPARIAN AND FOREST SPECIES OF AINSLIAEA (ASTERACEAE)

Understanding the role of habitat‐associated adaptation in reducing gene flow resulting in population differentiation and speciation is a major issue in evolutionary biology. We demonstrate a significant role for habitat divergence in species isolation between two naturally hybridizing riparian and nonriparian plants, Ainsliaea faurieana and A. apiculata (Asteraceae), on Yakushima Island, Japan. By analyzing the fine‐scale population structure at six sympatric sites, we found that variations in leaf shape, geography, light conditions, and genotype were strongly correlated across riverbank–forest transitions. No evidence of effective gene flow was found between the two species across the majority of the transition zones, although the NewHybrid clustering analysis confirmed interspecific hybridization. However, a relatively high level of gene flow was observed across one zone with a more diffuse ecotone and intermediate flooding and light conditions, possibly generated by human disturbances. These results suggest that the barriers to gene flow between the riparian and forest species are primarily ecological. Additional common garden experiments indicated that the two species are adaptively differentiated to contrasting flooding and light environments. Overall, our study suggests that adaptations to different habitats can lead to the formation of reproductive isolating barriers and the maintenance of distinct species boundaries.     

GENE FLOW AND SPECIES DELIMITATION: A CASE STUDY OF TWO PINE SPECIES WITH OVERLAPPING DISTRIBUTIONS IN SOUTHEAST CHINA

Species delimitation detected by molecular markers is complicated by introgression and incomplete lineage sorting between species. Recent modeling suggests that fixed genetic differences between species are highly related to rates of intraspecific gene flow. However, it remains unclear whether such differences are due to high levels of intraspecific gene flow overriding the spread of introgressed alleles or favoring rapid lineage sorting between species. In pines, chloroplast (cp) and mitochondrial (mt) DNAs are normally paternally and maternally inherited, respectively, and thus their relative rates of intraspecific gene flow are expected to be high and low, respectively. In this study, we used two pine species with overlapping geographical distributions in southeast China, P. massoniana and P. hwangshanensis, as a model system to examine the association between organelle gene flow and variation within and between species. We found that cpDNA variation across these two pine species is more species specific than mtDNA variation and almost delimits taxonomic boundaries. The shared mt/cp DNA genetic variation between species shows no bias in regard to parapatric versus allopatric species’ distributions. Our results therefore support the hypothesis that high intraspecific gene flow has accelerated cpDNA lineage sorting between these two pine species.     

THE HISTORICAL PATTERN OF GENE FLOW AMONG MIGRATORY AND NONMIGRATORY POPULATIONS OF PRAIRIE WARBLERS (AVES: PARULINAE)

Within a group of interbreeding organisms, the balance of gene flow among populations and microevolutionary forces acting within populations is expected to result in clinal transitions in the phenotypes possessed by members of differentiated populations. Discontinuous variation between geographically adjacent populations suggests the presence of a significant barrier to gene flow. Here I present genetic evidence for restricted gene flow between migratory and nonmigratory populations of prairie warblers. The nonmigratory form of this species is restricted to coastal mangroves in Florida and is morphologically distinguishable from the typical, migratory form that occurs across the remainder of the eastern United States. Pairs of migratory populations exhibited little population subdivision (Φ_ST ? 0.09), whereas pairs of migratory and nonmigratory populations are much more differentiated (Φ_ST = 0.27–0.42). A phylogenetic analysis of mitochondrial DNA haplotypes did not offer evidence of long-term isolation of migratory and nonmigratory populations. Together with the population genetic analysis, the phylogenetic relationship of haplotypes suggests that isolation between these forms must have arisen relatively recently in their history. Evidence for significant population structure is unexpected, given the geographic proximity of migratory and nonmigratory populations, the capacity for long-distance movements (e.g., migration) by prairie warblers, and several previous studies of population structure in North American birds. However, the findings are consistent with the geographic distribution of morphological and behavioral variation and demonstrate that significant boundaries between populations of vagile organisms may be relatively cryptic.     

MONOMORPHISM,REDUCED GENE FLOW,AND CLEISTOGAMY IN RARE AND COMMON SPECIES OF LESPEDEZA (FABACEAE)

Population genetic structure was analyzed in the rare, native prairie legume Lespedeza leptostachya Engelm. and in the widespread L. capitata Michx. Both species produce a mixture of chasmogamous and cleistogamous flowers. Allozymes were analyzed for 32 loci from 224 individuals from 12 populations of L. leptostachya and for 34 loci in 291 individuals from 12 populations of L. capitata. L. leptostachya is entirely monomorphic at all loci studied, while L. capitata shows strong among-population differentiation for the limited variation that occurs in that species. Allozyme data suggest that the level of gene flow among populations of L. capitata is very low, and that very low levels of outcrossing are effected by the chasmogamous flowers in L. capitata.     

扇形游仆虫和红色伪角毛虫丙氨酸转氨酶基因的分子克隆与序列分析

{{@ convertAbstractHtml(article.abstractinfoCn, "cn")}}       

飞虱科(半翅目)二新种和中国大陆二新纪录种

描记了采自云南西双版纳的飞虱科(半翅目)2新种:弯突淡背飞虱,新种Sogatellana curva sp.nov和刺茎奇臀飞虱,新种Miranus spinaphallus sp.nov.新种弯突淡背飞虱Sogatellana curva sp.nov与属内其它种的区别在于该种的阳基侧突略为弯曲,端部细且外端角不突出,而其它种阳基侧突的内端角和外端角都突出,另外该种膈背缘的形状不同于属内其它种;新种刺茎奇臀飞虱Miranusspinaphallus sp.nov.以阳茎左侧背面有一排刺状突起以及右腹侧面有一排小刺可与奇臀飞虱属其它种类相区别.报道了我国大陆的两个新纪录种,迪索西飞虱Syndelphax disonymos(Kirkaldy)(分布:广东湛江)和蜿蜒茎刺飞虱Sinolacme sinuosa Yang(分布:广西阳朔).新种的模式标本和其它研究标本均保存在中国科学院动物研究所标本馆.     

花蝽科中国二新种及二新纪录种记述(半翅目,异翅亚目)

记述花蝽科Anthocoridae2新种:阿拉善肩花蝽Tetraphleps alashanensis sp.nov.,五斑肩花蝽Tetraphleps maculatus sp.nov.,小花蝽属Orius Wolff中国2新纪录种:细鞭小花蝽Orius laticollis laticollis(Reuter),西伯利亚小花蝽Oriussibiricus Wagner。模式标本保存于内蒙古师范大学昆虫研究所标本馆。     

鸟类核型研究 Ⅲ.莺亚科和鹟亚科14种

本文报道了莺亚科10种和鹟亚科4种的核型,并对已报道过的这二亚科18种鸟类的核型进行了比较研究。这两个亚科通常依据表型差异被划分在一个科——鹟科中。Siblcy(1988)等则依据DNA差异(通过DNA—DNA分子杂交测定)将它们划在不问的三个科(鹟科、莺科和戴菊科)中。核型比较的结果支持了Sibley对这两亚科鸟类的重新划分。     

GENETIC VARIATION IN SYMPATRIC AND ALLOPATRIC POPULATIONS OF HYBRIDIZING FRESHWATER SNAIL SPECIES(VIVIPARUS ATER AND V. CONTECTUS)

To estimate the geographical extent of introgression, we studiedthe genetic structure of sympatric and allopatric populationsof hybridizing freshwater snail species Viviparus ater and V.contectus in central Europe. Six allozyme loci which were variablein Lake Garda, Italy in a previous study (five nearly diagnosticloci between the two species and one highly polymorphic locusin V. contectus) were analyzed from ten sympatric locationsand four allopatric populations each for the two species. Presumablyintrogressed genes (low allele frequencies) were found fromat least one locus in seven out of the ten sympatric sites.These seven sites covered most of northern Italy. The data indicatethat introgression has occurred from Viviparus contectus toV. ater and vice versa. Therefore, there is a possibility ofwidespread introgression or mosaic zones in nature. However,we cannot rule out that the observed patterns are due to theshared ancestry. V. ater possessed low genetic variation (thejackknifed mean of Wright's F_ST±S.E. over four loci was0.041±0.004). On the other hand, V. contectus showedhigh genetic differentiation (the jackknifed mean of F_ST±S.E. over six loci was 0.546±0.166). Although introgressionmay have caused evolutionary changes in V. ater and V. contectus,it was not strong enough to level out the genetic differencesbetween the two species, which may have originated from isolationamong populations in V. contectus and a past bottleneck eventin V. ater. (Received 21 June 1996; accepted 31 January 1997)     

SPATIAL RELATIONSHIP AND GENE FLOW PATHS BETWEEN POPULATIONS OF THE ALPINE SNAIL ARIANTA ARBUSTORUM (PULMONATA: HELICIDAE)

The alpine snail Arianta arbustorum (Pulmonata, Helicidae) was sampled along an 8-km contour line in the Swiss Alps orthogonal to well-known altitudinal clines in life history and shell traits. The allele frequencies and the morphs of 16 populations were compared with habitats. Two hypotheses about the recolonization of the mountain slopes after the last glaciation were tested against the present population structure. The allele frequencies are best explained by the spatial structure whereas the morphs are better explained by the habitats. The genotypic distances point to a historical explanation by recolonization processes, the phenotypical dissimilarities to a selectionistic interpretation of the present distribution of the populations. The following conclusions are from the study of the spatial relationship and the gene flow paths for A. arbustorum at Mount Martegnas: (1) Streams are the main gene flow paths; gene flow is low, allowing differentiation of local populations. (2) Morphs and habitats are correlated, but the interpretation has to be made carefully. The spatial dependence of habitats has to be included into the analysis of selection. (3) The random assumption for gene flow of A. arbustorum is not a spatial isolation-by-distance model, but a functional isolation-by-distance model, assuming gene flow over the drainage system.     

FLOW CYTOMETRIC ANALYSES OF VIRAL INFECTION IN TWO MARINE PHYTOPLANKTON SPECIES, MICROMONAS PUSILLA (PRASINOPHYCEAE) AND PHAEOCYSTIS POUCHETII (PRYMNESIOPHYCEAE)

Cell characteristics of two axenic marine phytoplankton species, Micromonas pusilla (Butscher) Manton et Parke and Phaeocystis pouchetii (Hariot) Lagerheim, were followed during viral infection using flow cytometry. Distinct differences between noninfected and infected cultures were detected in the forward scatter intensities for both algal species. Changes in side scatter signals on viral infection were found only for P. pouchetii. Chlorophyll red fluorescence intensity per cell decreased gradually over time in the infected cultures. DNA analyses were performed using the nucleic acid–specific fluorescent dye SYBR Green I. Shortly after infection the fraction of algal cells with more than one genome equivalent increased for both species because of the replication of viral DNA in the infected cells. Over time, a population of algal cells with low red autofluorescence and low DNA fluorescence developed, likely representing algal cells just prior to viral lysis. The present study provides insight into basic virus–algal host cell interactions. It shows that flow cytometry can be a useful tool to discriminate between virus infected and noninfected phytoplankton cells.     

THE INTERFACE BETWEEN PHYLOGENETICS AND POPULATION GENETICS: INVESTIGATING GENE TREES,SPECIES TREES,AND POPULATION DYNAMICS IN THE PHYLLOPHAGA FRATERNA SPECIES GROUP

This study uses traditional and contemporary phylogenetic and population genetic analyses to assess the causes of discordance (i.e., lineage sorting and introgression) among mitochondrial and nuclear gene trees for a clade of eastern North American scarab beetles (fraterna species group, genus Phyllophaga). I estimated gene trees using individual and combined analysis of one mitochondrial and two nuclear loci in MrBayes , and inferred a species tree using a hierarchical coalescent approach based on all loci in the program Best . Because hybridization violates the assumptions of Best , I tested for introgression by comparing species monophyly between the mitochondrial and nuclear gene trees based on the prediction that cytoplasmic genomes introgress more readily than nuclear genomes. Haplotype exclusivity was identified using Bayesian tests of monophyly and the genealogical sorting index. I used the results of the phylogenetic analyses and monophyly tests to develop an explicit hypothesis of introgression that could be tested in the program IMa. Results from these analyses provided evidence for introgression across clades within the fraterna group. The tiered analytical approach used in this study demonstrated how the use of multiple methods can identify when assumptions are violated and methods are prone to yield misleading results.     

锦织蛾属二新种和中国二新纪录种(鳞翅目,织蛾科)(英文)

记述锦织蛾属PromalactisMeyrick4个种,包括新种掌锦织蛾P.palmata sp.nov.和仿丽头锦织蛾P.subcolacephala sp.nov.,中国新纪录种西锦织蛾P.sineviLvovsky,1986和斯锦织蛾P.svetlanaeLvovsky,1985。文中提供了每个种的成虫和外生殖器特征图。掌锦织蛾,新种Promalactis palmata sp.nov.(图1,5,9)本种外形和外生殖器特征与显锦织蛾P.epistactaMeyrick,1908近似,但本种雄性外生殖器抱器背基部突起呈叶状,抱器瓣端部1/3近似掌状,阳茎基环侧叶末端达背兜2/3处,阳茎具2枚角状器。显锦织蛾P.epistacta雄性外生殖器抱器背基部突起柳条状,抱器瓣端部钩状,阳茎基环侧叶不明显,阳茎具1枚角状器。正模♂,贵州省绥阳县宽阔水保护区,海拔1500m,2010-08-12,杨琳琳采,外生殖器玻片号DZH10243。副模:2♂♂,3♀♀,2010-08-10~13,其它资料同正模,1♂,1♀,2010-08-16,杜喜翠采,采集地点同正模(外生殖器玻片号DZH10244♀,DZH10245♀,DZH10247♂,DZH10249♀,DZH10250♂)。分布:中国(贵州)。仿丽头锦织蛾,新种Promalactis subcolacephala sp.nov.(图2,6)本种与丽头锦织蛾P.colacephalaWang,LietZheng,2000非常近似,但雄性外生殖器抱器瓣末端背面有1钩状突起,阳茎末端无刺状突起。丽头锦织蛾P.colacephala雄性外生殖器抱器瓣末端背面有1直的突起,阳茎末端有1弯曲的刺状突起。正模♂,贵州省绥阳县宽阔水保护区,海拔840m,2010-06-09,杨琳琳采,外生殖器玻片号DZH10335。副模1♂,2010-06-08,其它资料同正模(外生殖器玻片号DZH10253)。分布:中国(贵州)。西锦织蛾Promalactis sineviLvovsky,1986中国新纪录(图3,7,10)Promalactis sineviLvovsky,1986:39.分布:中国(北京、河北、山西、辽宁);俄罗斯(远东)。斯锦织蛾Promalactis svetlanaeLvovsky,1985中国新纪录(图4,8,11)Promalactis svetlanae Lvovsky,1985:101;ParketPark,1998:57.分布:中国(黑龙江);俄罗斯(远东)。     

GENETICAL POPULATION STRUCTURE IN PLANTS: GENE FLOW BETWEEN DIPLOID SEXUAL AND TRIPLOID ASEXUAL DANDELIONS (TARAXACUM SECTION RUDERALIA)

Levels and distribution of genetic variation were studied in central and western European populations of Taraxacum section Ruderalia containing differing mixtures of sexual diploid and asexual triploid plants. All sexual populations were panmictic with their variation partitioned mainly among populations. Genotypic diversity in triploid samples was very high with few clones widespread and many clones restricted to one or a few populations. Extensive amounts of gene (pollen) flow between the diploid and triploid components of a population were inferred from the following data: (1) the two ploidy levels share all major allozyme polymorphisms; (2) the intrapopulational homogeneity in genic variation between diploids and triploids contrasts strongly with the geographic differentiation at each ploidy level separately; (3) population-unique alleles simultaneously occur at the two ploidy levels; (4) not only sexuals but also asexuals generally simulate Hardy-Weinberg expectations. Most likely, intrapopulational gene exchange occurs bidirectionally by mechanisms such as reductional pollen meiosis in apomictic plants, facultative apomixis, and formation of unreduced gametes in sexuals. Thus, diploid and triploid Taraxacum section Ruderalia are less genetically isolated than has previously been supposed and probably form a cohesive evolutionary unit with the level at which gene pools are shared differing by population.     

TWO MORPHOLOGICALLY SIMILAR BIOLOGICAL SPECIES: CHONDRUS PINNULATUS AND C. ARMATUS (GIGARTINACEAE,RHODOPHYTA)1

The nature of the relationship between Chondrus pinnulatus (Harvey) Okamura f. pinnulatus and C. pinnulatus f. armatus (Harvey) Yamada et Mikami (Gigartinaceae, Rhodophyta) was investigated by comparative analysis of their biogeography, phenologies, life histories, gross and vegetative morphology, crossability, and upper thermal tolerance. The forma pinnulatus has a more northerly distribution in Japan and adjacent waters, exhibiting adaptation to the cooler regions, whereas the forma armatus has a more southerly range. The latter may be the result of a higher thermal tolerance. Both formae have a Polysiphonia-type life history and are similar in their internal vegetative morphology. They can, however, be distinguished by gross morphology: forma pinnulatus has wide, flattened axes, compressed to flattened ultimate segments and proliferations, while forma armatus has narrow, compressed to subterete axes and subterete to terete ultimate segments and proliferations. These differences persist in laboratory culture. All intraformae crosses were positive, with carpospores from the cross developing into fertile F₁ tetrasporophytes releasing tetraspores that developed into dioecious F₁ gametophytes, the female gametophytes of which formed normal cystocarps. This suggests that members of populations of each forma freely interbreed. Among interformae crosses, only some offspring derived from geographically distant strains bore normal cystocarps in the F₁ female gametophytes. Other crosses showed that interbreeding between populations of these two formae was blocked by various isolating mechanisms: incompatibility, hybrid inviability, and hybrid sterility. Reproductive isolation between f. pinnulatus and f. armatus is virtually complete in wild populations, because hybrid populations have not been found in the wild. In addition, these two entities can be considered biological species that are also referred to the taxonomic species, C. armatus and C. pinnulatus, because they do not overlap with regard to the morphology of the ultimate segments and proliferations. Subtle (but significant) gross morphological differences, partial interfertility between the two species, and deleterious hybridization in the area in which they occur sympatrically suggest that their evolutionary divergence was relatively recent.     

TWO NEW AND TWO KNOWN SPECIES OF TYPHLODROMUS SCHEUTEN (ACARI: PHYTOSEIIDAE) FROM AUSTRALIA

Abstract
The females of Typhlodromus elisae sp. n., T. haramotoi Prasad, T. neobakeri Prasad, and both sexes of T. steeli sp. n. are described and figured.     

中国蝇科二新属二新种（双翅目：蝇科）

整理新疆地区蝇科标本中,发现二新属和二新种,其中花秽蝇属,新属Ａｎｔｈｏｃｏｅｎｓｉａ ｇｅｎ．ｎｏｖ．隶于秽蝇亚科Ｃｏｅｎｓｏｉｉｎａｅ,叉棘蝇属,新属Ｃｒｕｓｉａｅｌｌａ ｇｅｎ．ｎｏｖ．隶于棘蝇亚科Ｐｈａｏｎｉｉｎａｅ。新种模式标本保存于沈阳师范学院生物系。