Plants Used for Reproductive Health by Nahua Women in Northern Veracruz,Mexico

Plants Used for Reproductive Health by Nahua Women in Northern Veracruz, Mexico. This paper reports the use of medicinal plants by Nahua women in the state of Veracruz, Mexico. It documents the women’s plant knowledge for reproductive purposes, which includes uses such as conception, pregnancy, birth, contraception, menstruation, post-partum, and general reproductive health. The concept of equilibrium is very important in regaining health among the Nahua; consequently, many of the medicinal plants have this as their primary purpose. The introduction of biomedical clinics and hospitals in the region has had a significant effect on the loss of knowledge about medicinal plants. Additionally, the midwives are not taking any new apprentices and laywomen are not passing on their knowledge to future generations. This generational gap contributes to the loss of knowledge about medicinal plants. This research contributes to the study of indigenous ethnobotany by (a) creating a record of the plant knowledge possessed by indigenous women, (b) giving voice to some of their health concerns, (c) indicating how the introduction of biomedicine has affected their plant use, and (d) providing a framework for understanding how marginal peoples around the world respond to the impact that globalization and change has on their health needs and local ethnobotanical knowledge.     

Understanding Interrelationships Among Predictors (Age,Gender, and Origin) of Local Ecological Knowledge<Superscript>1</Superscript>

Understanding Interrelationships among Predictors (Age, Gender, and Origin) of Local Ecological Knowledge. Understanding which factors predict local ecological knowledge can provide insight into how this knowledge is learned and how it may change in the future. We assess how knowledge of both useful plants and plant natural history vary according to gender, age, and origin in mestizo communities in Venezuela’s Caura Basin. Two sets of structured questionnaires were carried out with a total of 83 adults in three communities. Multiple regression analyses were used to identify the predictors of knowledge of 6 plant-use categories and natural history knowledge of 12 plant species. Gender, age, and origin (≥2 generations in the Caura vs. foreign-born) were all important predictors of knowledge of useful plants and natural history; however, their importance differed between the two types of knowledge. Origin was a more important predictor of knowledge of useful plants, whereas age was more important in predicting knowledge of natural history. This suggests differences in how each type of knowledge is learned and transmitted. Gender was an important predictor variable in most models, reflecting gender roles in the Caura mestizo communities. Also, for most categories of plant-use knowledge, the interactions among predictors were significant, indicating that the effect of one variable depended on the level of the other. These results illustrate how overlooking interactions among variables, as most studies to date have done, can risk misinterpretation of results by simplifying complex situations.     

Medicinal plants used by women in Mecca: urban,Muslim and gendered knowledge

Background

This study explores medicinal plant knowledge and use among Muslim women in the city of Mecca, Saudi Arabia. Ethnobotanical research in the region has focused on rural populations and male herbal healers in cities, and based on these few studies, it is suggested that medicinal plant knowledge may be eroding. Here, we document lay, female knowledge of medicinal plants in an urban centre, interpreting findings in the light of the growing field of urban ethnobotany and gendered knowledge and in an Islamic context.

Methods

Free-listing, structured and semi-structured interviews were used to document the extent of medicinal plant knowledge among 32 Meccan women. Vernacular names, modes of preparation and application, intended therapeutic use and emic toxicological remarks were recorded. Women were asked where they learnt about medicinal plants and if and when they preferred using medicinal plants over biomedical resources. Prior informed consent was always obtained. We compared the list of medicinal plants used by these Meccan women with medicinal plants previously documented in published literature.

Results

One hundred eighteen vernacular names were collected, corresponding to approximately 110 plants, including one algae. Of these, 95 were identified at the species level and 39 (41%) had not been previously cited in Saudi Arabian medicinal plant literature. Almost one half of the plants cited are food and flavouring plants. Meccan women interviewed learn about medicinal plants from their social network, mass media and written sources, and combine biomedical and medicinal plant health care. However, younger women more often prefer biomedical resources and learn from written sources and mass media.

Conclusions

The fairly small number of interviews conducted in this study was sufficient to reveal the singular body of medicinal plant knowledge held by women in Mecca and applied to treat common ailments. Plant availability in local shops and markets and inclusion in religious texts seem to shape the botanical diversity used by the Meccan women interviewed, and the use of foods and spices medicinally could be a global feature of urban ethnobotany. Ethnobotanical knowledge among women in Islamic communities may be changing due to access to mass media and biomedicine. We recognise the lack of documentation of the diversity of medicinal plant knowledge in the Arabian Peninsula and an opportunity to better understand gendered urban and rural knowledge.

     

A Comparative Analysis of Nineteenth Century Pharmacopoeias in the Southern United States: A Case Study Based on the Gideon Lincecum Herbarium<Superscript>1</Superscript>

A Comparative Analysis of Nineteenth Century Pharmacopoeias in the Southern United States: A Case Study Based on the Gideon Lincecum Herbarium. The Gideon Lincecum Herbarium represents the pharmacopoeia of Dr. Gideon Lincecum, a botanical physician practicing in Mississippi and Texas during the first half of the nineteenth century. The herbarium contains 313 specimens representing 309 species, 242 genera, and 96 families, and includes ethnobotanical annotations for 286 medicinal taxa. The collection data provided by Lincecum indicate that the specimens were collected between 1835 and 1852. With the exception of 22 specimens considered by Campbell (1951), this is the first study to place this pharmacopoeia in a historical context. Taxonomic determinations of the herbarium specimens were confirmed or corrected. Comparative analyses were conducted to investigate the relationship of Lincecum’s pharmacopoeia to those of six other medical traditions practiced in the southern United States during the nineteenth century. Cluster analyses based on Jaccard co-efficient placed the historical pharmacopoeias of medical traditions in the early nineteenth century into distinct Euro–American and American Indian groups. Despite the recognition of distinct allopathic and botanical medical traditions, an extensive overlap in the composition of their pharmacopoeias is observed. This may reflect the reliance of these traditions on allopathic principles and drugs of plant origin during the first half of the nineteenth century. In contrast, Cherokee, Chickasaw, Choctaw, and Creek pharmacopoeias show limited overlap with each other in composition despite a long history of interaction between these groups. Lincecum’s pharmacopoeia shares a larger Jaccard co–efficient value with the Choctaw pharmacopoeia than would be expected based on their placement in distinct Euro–American and American Indian groups in the dendrogram. The large proportion of Lincecum’s citations that reference Choctaw informants provides direct evidence for the incorporation of Choctaw medical knowledge and taxa into Lincecum’s pharmacopoeia. These data suggest that the composition of historical pharmacopoeias is influenced by both contemporary medical practices and the regional and cultural contexts in which the pharmacopoeias are utilized.     

Quantifying the Trade in Cycads (<Emphasis Type="Italic">Encephalartos</Emphasis> Species) in the Traditional Medicine Markets of Johannesburg and Durban,South Africa<Superscript>1</Superscript>

Quantifying the Trade in Cycads ( Encephalartos Species) in the Traditional Medicine Markets of Johannesburg and Durban, South Africa. Cycads have been used for traditional purposes for centuries, mainly as a source of starch during droughts and famines. In South Africa, Encephalartos species are traded for traditional medicine (TM) in local TM markets. The 2009 IUCN Red Data assessment for South African flora lists 78% of the 37 indigenous Encephalartos species as “Threatened”, with three species already “Extinct in the Wild” (EW). South African cycads face an extinction crisis, primarily due to collecting for the horticultural trade. The trade in Encephalartos for TM also has a significant impact on several species, and is largely understudied. In this paper, the trade in Encephalartos for TM was quantified in South Africa’s two largest TM markets, Faraday in Johannesburg and Warwick in Durban. Harvesting of South African Encephalartos for TM involves the removal of bark strips and/or whole stem sections. Encephalartos species were sold at 26% and 13% of the stalls at Faraday and Warwick, respectively, with an estimated 9.0 metric tons traded at Warwick in 2009. Stem samples purchased in the markets were assigned to stem diameter size classes using a size class chart, and regression analysis was used to validate the allocations. Most harvesting was from sub-adult and adult cycads, and it appears that bark strips are removed from large arborescent plants, whereas smaller individuals and subterranean-stemmed species are harvested by removing the entire plant. There is generally a positive relationship between stem diameter and leaf base length as well as stem diameter and pith radius. The former can be used as a predictor of stem diameter size class for market samples that prove difficult to assign to diameter classes using the chart. Overall, this is the first study to quantify the trade in Encephalartos for TM in South Africa with reference to the size classes of the plants traded.     

Ethno-medicinal plants of tribal people: A case study in Pakyong subdivision of East Sikkim,India

BackgroundTribal people of India still use ancient traditional health care practices, using available plants in their surroundings. Traditional knowledge of medicine is vanishing due to urbanization and outmigration thus its urgent need to preserve this valuable knowledge.MethodsThis recent study was carried out in Pakyong subdivision of East Sikkim during 2016–17, and information was collected interviewing 71 informants of the Lepcha, Bhotia, and Nepali tribes.ResultsPresent study documenting 60 useful plant species belonging to 38 families, used for local health care needs including ten veterinary useful species. Herbaceous plants were used in high proportion (32 species, 54%) followed by trees (20, 33%), shrubs (20, 8%) and climbers (3, 5%). Leaves were the most frequently used plant parts and decoction was the common drug formulation among the inhabitants. The Informant consensus factor (ICF) ranged between 0.88 and 0.97, and fidelity levels in the study region were varied from 11.53% to 100%.ConclusionDocumentation of local plant uses by tribal people will be helpful in sustainable use of indigenous practices of medicinal plants, and further information may be used for pharmacological investigation and novel phytoconstituents isolation.     

Cassava: constraints to production and the transfer of biotechnology to African laboratories

Knowledge and technology transfer to African institutes is an important objective to help achieve the United Nations Millennium Development Goals. Plant biotechnology in particular enables innovative advances in agriculture and industry, offering new prospects to promote the integration and dissemination of improved crops and their derivatives from developing countries into local markets and the global economy. There is also the need to broaden our knowledge and understanding of cassava as a staple food crop. Cassava (Manihot esculenta Crantz) is a vital source of calories for approximately 500 million people living in developing countries. Unfortunately, it is subject to numerous biotic and abiotic stresses that impact on production, consumption, marketability and also local and country economics. To date, improvements to cassava have been led via conventional plant breeding programmes, but with advances in molecular-assisted breeding and plant biotechnology new tools are being developed to hasten the generation of improved farmer-preferred cultivars. In this review, we report on the current constraints to cassava production and knowledge acquisition in Africa, including a case study discussing the opportunities and challenges of a technology transfer programme established between the Mikocheni Agricultural Research Institute in Tanzania and Europe-based researchers. The establishment of cassava biotechnology platform(s) should promote research capabilities in African institutions and allow scientists autonomy to adapt cassava to suit local agro-ecosystems, ultimately serving to develop a sustainable biotechnology infrastructure in African countries.     

Mapuche perceptions and conservation of Andean <Emphasis Type="Italic">Nothofagus</Emphasis> forests and their medicinal plants: a case study from a rural community in Patagonia,Argentina

In a Mapuche community situated in the sub-antarctic forest of the northwest of Argentine Patagonia, analysis was carried out on forest environmental perception and its relation to the resilience of the body of traditional botanical knowledge regarding medicinal plants. Data was obtained on the ethno-classification and differential use of the forest gathering environment with respect to its practical and cultural value. Semi-structured interviews were carried out with 30 randomly chosen inhabitants, and the data were analysed using qualitative methods and non-parametric statistics. Most citations (64.5%) were of species gathered in Nothofagus antarctica forest, 26.2% were of species from N. pumilio forest, and 20.3% referred to species from a mixed forest, with N. dombeyi. The forests studied have low values for similarity in terms of medicinal species richness, indicating a unique offer of therapeutic resources in each one. The use of the different forest types seems to be associated with the search for therapeutic resources for specific ailments. However, the redundancy of functions of species in each forest type can offer alternative remedies, which provides plant conservation, security and the possibility of reorganisation of their traditional medicinal knowledge. This case study showed the importance of considering folk systems and the role that this knowledge has played in plant resource management and forest protection. Different forests are used and valued differentially, not only with regard to usefulness but also in symbolic-religious terms, and together they fulfil a cushioning function, protecting holistically traditional botanical knowledge, people’s health and forests. It is of great importance, therefore, that conservation policies favouring environmental heterogeneity are implemented, and that local inhabitants participate in the development of management plans.     

Quantifying Medicinal Plant Knowledge among Non–Specialist Antanosy Villagers in Southern Madagascar

Quantifying Medicinal Plant Knowledge among Non–Specialist Antanosy Villagers in Southern Madagascar. Medicinal plant knowledge among non–specialist Antanosy villagers of southeastern Madagascar was investigated in a two–stage study. First, free–listing was used to collect the names of medicinal plants most familiar to local people. Data were organized by habitat and frequency into a short list of the 42 most frequently listed plants by habitat. A second group of interviewees were asked to name health conditions that could be treated with plants on the short list. Age, gender, and dwelling proximity to the forest were tested across the general habitat in which medicinal plants were found: in or near the village, in disturbed buffer areas between the village and the forest, or in the forest itself. Neither age nor gender was significant in free–listing. Naming health conditions treated with specific plants showed that knowledge increases with age and that for all but the oldest age group, women knew more plant uses than men. Women knew more plants from the village and buffer areas, and fewer from the forest than men. The proximity of the home to the forest had no influence on medicinal plant knowledge. The non–specialists interviewed named an average of 14 medicinal plants and most knew an average of 37 uses for 9 of the 42 most common medicinal plants. The most common conditions for people knew of plant treatments were stomach ache, babies’ fevers, and several unlisted conditions. Both exotic and endemic plant species were known to the non–specialists indicating that medicinal plant knowledge is being sustained and adapted to changes affecting both the people and their environment.     

Diversity of Plant Knowledge as an Adaptive Asset: A Case Study with Standing Rock Elders<Superscript>1</Superscript>

Diversity of Plant Knowledge as an Adaptive Asset: A Case Study with Standing Rock Elders. Indigenous knowledge is often represented as being homogeneous within cultural groups, and differences in knowledge within communities are interpreted as a lack of cultural consensus. Alternatively, differences in knowledge represent a range of possibilities for communities to respond to social and ecological change. This paper examines the diversity of plant knowledge among elders who live in the Standing Rock Nation of the northern Great Plains. Elders know how to use different plants, and also hold different knowledge about the same plants. Analysis indicates that elders each contribute unique, complementary, and seemingly contradictory plant knowledge to their community. Compiled seasonal rounds help visualize differences in knowledge about the temporal availability of plants. These differences are linked to variations in use, including references to specific gathering sites, strategies to harvest multiple species, and selection of plants at different stages of development. Elders’ diverse knowledge about the seasonal availability of plants may facilitate community adaptation to climate change in the 21st century.     

Changes in Assumption Day Herbal Bouquets in Poland: A Nineteenth Century Study Revisited<Superscript>1</Superscript>

Changes in Assumption Day Herbal Bouquets in Poland: A Nineteenth Century Study Revisited. The aim of this study is to compare a list of plants blessed in herbal bouquets on Assumption Day (August 15th) in Poland over a century ago with the bouquets blessed in the same locations in 2009. Assumption Day has been the main occasion when plants are blessed in Polish Roman Catholic churches for centuries. Such bouquets, composed of medicinal herbs and crop plants, are long believed to possess apotropaic properties. In 1894–1899, Seweryn Udziela made an herbarium documenting the composition of these bouquets (over 100 species) in 13 locations in the Kraków area. In 2009, 482 bouquets were photographed on Assumption Day and the plant species were identified. Results indicate that only half the species from Udziela’s list are still blessed. A total of 233 taxa were found in the bouquets in 2009, an average of nine species per bouquet. The proportion of cultivated ornamentals has increased over time, whereas the proportion of wild species and dry grassland plants has decreased. The proportion of crop plants, as well as species from grassland and forest habitats, remained the same. The most commonly blessed species were Solidago spp., Tanacetum vulgare, Dahlia sp., Sanguisorba officinalis, Anethum graveolens, Achillea millefolium, Mentha spp., Zinnia elegans, Triticum sp., Avena sativa, and Sorbus aucuparia. The tradition of blessing bouquets continues, although the composition of the plant species has changed. These changes are due to alterations in the surrounding vegetation and the species of plants in cultivation, the transformation of species’ value from medicinal and apotropaic, the maintenance of floral traditions, and artistic competition.     

Cultural Importance Indices: A Comparative Analysis Based on the Useful Wild Plants of Southern Cantabria (Northern Spain)<Superscript>1</Superscript>

Cultural Importance Indices: A Comparative Analysis Based on the Useful Wild Plants of Southern Cantabria (Northern Spain). This paper compares four indices based on informant consensus. Each index aims to assess the cultural significance of plant species and is suitable for statistical testing of different hypotheses. For the comparison, we used data concerning plants traditionally used in the Campoo area of southern Cantabria in northern Spain. Our results show a positive and significant correlation between the number of uses (NU) and the frequency of citation (FC) of the species. It seems to be a general rule that the more versatile a plant, the more widespread its usefulness. In addition, NU is highly influenced by the number of use-categories in the study. Consequently, an objective index must rely on FC more than NU. We propose the use of the cultural importance index (CI), which is defined as the summation of the informants’ proportions that mention each of the uses of the species. The CI index is highly correlated with FC and, although it also considers diversity of use, each use-category is conveniently weighted by the number of informants mentioning it. Despite the use of cultural significance indices being questioned, we believe that indices based on in-depth, semi-structured interviews are still very useful for compilation studies of passive knowledge, such as most ethnobotanical works conducted in the last three decades in Europe.     

Yao herbal medicinal market during the Dragon Boat Festival in Jianghua County,China

Background

The traditional medicinal markets held during the Dragon Boat Festival are common and important in China’s countryside. In Jianghua, a Yao autonomous county in Hunan Province in China, the medicinal market also plays an important role for the application, conservation, and communication of traditional Yao medicinal knowledge.

Methods

During the Dragon Boat Festival in 2016 and 2017, ethnobotanical surveys and inventories were conducted in the medicinal market of Jianghua County, and voucher plant specimens were collected, identified, and deposited in a herbarium. Quantitative analysis included measurement of frequency of occurrence for species in the marketplace and the relative importance index for the number of uses for a given species.

Results

A total of 306 plant species (249 genera, 113 families) and their related information about the medicinal market were collected. Some major findings include the following: (1) Using the whole plant as medicine is more common than other medicinal plant parts; (2) treating rheumatism and clearing inner heat are the most frequent medicinal uses; and (3) taking a medicinal bath is the most frequent modality to administer the traditional medicine. The frequency of occurrence and the relative importance index of some medicinal plants were analyzed, as well as the demographics and the number of stalls and the status of traditional Yao medicinal knowledge in Jianghua. Based on the investigation, suggestions were proposed for better protecting the medicinal market and preserving traditional medicinal knowledge in Jianghua County.

Conclusion

The medicinal market during the Dragon Boat Festival in Jianghua County possesses an important cultural value and helps to conserve the traditional Yao medicinal knowledge. The medicinal plants sold at the market showed great diversity and unique local characteristics. The medicinal market is facing some challenges in such a rapidly developing era. Cultivation of young healers and maintaining the local biodiversity might be the key solutions for the development of local medicinal market and local Yao medicinal knowledge.

     

Mixed Methods Survey of Zoonotic Disease Awareness and Practice among Animal and Human Healthcare Providers in Moshi,Tanzania

BackgroundZoonoses are common causes of human and livestock illness in Tanzania. Previous studies have shown that brucellosis, leptospirosis, and Q fever account for a large proportion of human febrile illness in northern Tanzania, yet they are infrequently diagnosed. We conducted this study to assess awareness and knowledge regarding selected zoonoses among healthcare providers in Moshi, Tanzania; to determine what diagnostic and treatment protocols are utilized; and obtain insights into contextual factors contributing to the apparent under-diagnosis of zoonoses.Methodology/ResultsWe conducted a questionnaire about zoonoses knowledge, case reporting, and testing with 52 human health practitioners and 10 livestock health providers. Immediately following questionnaire administration, we conducted semi-structured interviews with 60 of these respondents, using the findings of a previous fever etiology study to prompt conversation. Sixty respondents (97%) had heard of brucellosis, 26 (42%) leptospirosis, and 20 (32%) Q fever. Animal sector respondents reported seeing cases of animal brucellosis (4), rabies (4), and anthrax (3) in the previous 12 months. Human sector respondents reported cases of human brucellosis (15, 29%), rabies (9, 18%) and anthrax (6, 12%). None reported leptospirosis or Q fever cases. Nineteen respondents were aware of a local diagnostic test for human brucellosis. Reports of tests for human leptospirosis or Q fever, or for any of the study pathogens in animals, were rare. Many respondents expressed awareness of malaria over-diagnosis and zoonoses under-diagnosis, and many identified low knowledge and testing capacity as reasons for zoonoses under-diagnosis.ConclusionsThis study revealed differences in knowledge of different zoonoses and low case report frequencies of brucellosis, leptospirosis, and Q fever. There was a lack of known diagnostic services for leptospirosis and Q fever. These findings emphasize a need for improved diagnostic capacity alongside healthcare provider education and improved clinical guidelines for syndrome-based disease management to provoke diagnostic consideration of locally relevant zoonoses in the absence of laboratory confirmation.     

Returns from Harvesting a Commercial Non-timber Forest Product and Particular Characteristics of Harvesters and Their Strategies: <Emphasis Type="Italic">Aquilaria crassna</Emphasis> and Agarwood in Lao PDR<Superscript>1</Superscript>

Returns from Harvesting a Commercial Non-timber Forest Product andParticular Characteristics of Harvesters and Their Strategies: Aquilaria crassna and Agarwood in Lao PDR. This paper explores associations between the returns from harvesting a commercial non-timber forest product and particular characteristics of harvesters and their strategies. Data originate from a survey in Laos of the harvest and trade of agarwood, a highly valuable commodity harvested mainly from tree species in the genus Aquilaria. Five groups of harvesters were identified, with considerable differences in returns between them. Foreign harvesters, i.e., ethnic Vietnamese, earned approximately USD 13 per day on average, while nonlocal full-time harvesters earned approximately USD 9, and nonlocal part-time harvesters USD 7 per day. Local people earn approximately USD 5 per day and only USD 4 if harvesting only took place in village forests. Factors observed to determine returns are market access, especially access to national and international markets, and knowledge and experience in trading, harvesting, and the organization of harvesting trips. It is suggested that interventions should be made to address these factors, to level out returns from harvesting, and to increase the share of returns captured by local people.      

Blended divergences: local food and medicinal plant uses among Arbëreshë, Occitans,and autochthonous Calabrians living in Calabria,Southern Italy

Abstract

A study focusing on traditional uses of wild plants for gastronomic and medicinal purposes was carried out among three linguistic communities in Calabria, southern Italy. Ninety interviews with local elderly informants were conducted among Occitans and Arbëreshë, two linguistic minorities, and the dominant culture of autochthonous Calabrians. We recorded 85 taxa belonging to 39 botanical families and 66 different detailed use-reports including 35 culinary and 31 medicinal uses. Our overall data show the permanence of traditional ecological knowledge related to wild and semi-domesticated food and medicinal plants; however, high similarity indices among the three communities demonstrate that traditional ecological knowledge is following the pathway of homogenization and standardization toward the dominant culture, facilitated by a context of linguistic erosion, limited intergenerational transmission, and a centuries-old diffusion with Calabrian culture. Moreover, our study calls for further field surveys in isolated areas of Calabria to analyze how traditional ecological practices can be key tools in the development of local small-scale economies through the promotion of artisanal food entrepreneurship of wild food plant transformation.     

Spatial and management factors associated with exposure of smallholder dairy cattle in Tanzania to tick-borne pathogens

A cross-sectional study of serum antibody responses of cattle to tick-borne pathogens (Theileria parva, Theileria mutans,Anaplasma marginale, Babesia bigemina and Babesia bovis) was conducted on smallholder dairy farms in Tanga and Iringa Regions of Tanzania. Seroprevalence was highest for T. parva (48% in Iringa and 23% in Tanga) and B. bigemina (43% in Iringa and 27% in Tanga) and lowest for B. bovis (12% in Iringa and 6% in Tanga). We use spatial and non-spatial models, fitted using classical and Bayesian methods, to explore risk factors associated with seroprevalence. These include both fixed effects (age, grazing history and breeding status) and random effects (farm and local spatial effects). In both regions, seroprevalence for all tick-borne pathogens increased significantly with age. Animals pasture grazed in the 3 months prior to the start of the sampling period were significantly more likely to be seropositive for Theileria spp. and Babesia spp. Pasture grazed animals were more likely to be seropositive than zero-grazed animals for A. marginale, but the relationship was weaker than that observed for the other four pathogens. This study did not detect any significant differences in seroprevalence associated with other management-related variables, including the method or frequency of acaricide application. After adjusting for age, there was weak evidence of localised (<5 km) spatial correlation in exposure to some of the tick borne diseases. However, this was small compared with the 'farm-effect', suggesting that risk factors specific to the farm were more important than those common to the local neighbourhood. Many animals were seropositive for more than one pathogen and the correlation between exposure to the different pathogens remained after adjusting for the identified risk factors. Identifying the determinants of exposure to multiple tick-borne pathogens and characterizing local variation in risk will assist in the development of more effective control strategies for smallholder dairy farms.     

Distribution patterns and industry planning of commonly used traditional Chinese medicinal plants in China

Medicinal plants are the primary material basis for disease prevention and treatment in traditional Chinese medicine (TCM). The conservation and sustainable utilization of these medicinal plants is critical for the development of the TCM industry. However, wild medicinal plant resources have sharply declined in recent decades. To ameliorate the shortage of medicinal plant resources, it is essential to explore the development potential of the TCM industry in different geographical regions. For this purpose, we examined the spatial distribution of commonly used medicinal plants in China, the number of Chinese medicinal material markets, and the number of TCM decoction piece enterprises. Specifically, multispecies superimposition analysis and Thiessen polygons were used to reveal the optimal range for planting bulk medicinal plants and the ideal regions for building Chinese medicinal material markets, respectively. Furthermore, we quantitatively analyzed mismatches between the spatial distribution of commonly used medicinal plant richness, Chinese medicinal material markets, and TCM decoction piece enterprises. We found that the areas suitable for growing commonly used medicinal plants in China were mainly distributed in Hengduan Mountain, Nanling Mountain, Wuling Mountain, and Daba Mountain areas. The Thiessen polygon network based on Chinese medicinal material market localities showed there are currently fewer markets in southwestern, northwestern, and northeastern China than in central and southern China. TCM decoction piece enterprises are concentrated in a few provinces, such as Hebei and Jiangxi. We found that the distribution of commonly used medicinal plants, Chinese medicinal material markets and TCM decoction piece enterprises are mismatched in Henan, Shaanxi, Hunan, Hubei, Zhejiang, Fujian, Chongqing, and Xizang. We recommend strengthening development of the TCM industry in Henan, Hunan, Zhejiang, Shaanxi, Hubei, Chongqing, Fujian, and Xizang; building more Chinese medicinal material markets in southwestern, northwestern, and northeastern China; and establishing medicinal plant nurseries in resource-rich provinces to better protect and domesticate local medicinal plants.     

Electrogenic plasma membrane H<Superscript>+</Superscript>-ATPase activity using voltage sensitive dyes

Fast responding voltage sensitive dyes, RH421 and di-4-ASPBS, were used to study the electrogenic properties of plant plasma membrane proton pumps on sealed plasma membrane vesicles extracted by two-phase partitioning from Beta vulgaris and Avena sativa cv Swan root material. Fluorescence spectroscopy in the presence of the dye RH421 (10.8 nM) was sufficiently sensitive to detect electrogenic activity of the extracted plant vesicles. The dye detection system could detect inhibition of electrogenic activity of vesicles by vanadate (75 μM) and stimulation by nigericin (0.5 μM). The newly developed dye di-4-ASPBS was less sensitive to detecting the electrogenic proton pump activity. This study represents an important innovation in plant biophysics as this class of fast responding voltage sensitive dyes have never to our knowledge been used to study electrogenic proton pump activity derived from plant membranes and represents a novel approach for carrying out such studies.     

Characterization of Farmer’s knowledge and management practices of papaya mealybug Paracoccus magnatus (Hemiptera: Pseudococcidae) in Tanzania

Papaya mealybug (PMB) is a serious insect pest for papaya production in Sub-Saharan Africa, limiting production potential in farming communities. We did a household survey to evaluate the Characteristics of farmers'' knowledge, challenges, and current (PMB) control practices in four papaya growing regions of Tanzania namely, Tanga, Dodoma, Pwani, and Katavi involving 100 papaya farmers. The study found that 96% of farmers reported PMB, as a major challenge in papaya production. Very few (0.8%) of the farmers were knowledgeable on insect pest identification. Chemical pesticides were the only option for PMB control, and 43.0% of farmers were able to access and apply. We also found that 36.4% of the farmers were aware of the adverse effects of chemical pesticides. Furthermore, the study observed that 0.3% of farmers use botanical pesticides. Additionally, the study observed that 44.1% of farmers use control measures against PMB, the remaining 55.9% did not practice any control measure, thus leading to low papaya yields observed in the study regions. Our findings provide insights to farmers into the use of plant-based pesticides, mainly plant essential oils, and its benefits that may promote farmers'' attitudes towards increasing papaya yield and reducing chemical pesticide use to avoid pest resistance.