首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 234 毫秒
1.
Summary The compositional properties of DNAs from 122 species of fishes and from 18 other coldblooded vertebrates (amphibians and reptiles) were compared with those from 10 warm-blooded vertebrates (mammals and birds) and found to be substantially different. Indeed, DNAs from cold-blooded vertebrates are characterized by much lower intermolecular compositional heterogeneities and CsCl band asymmetries, by a much wider spectrum of modal buoyant densities in CsCl, by generally lower amounts of satellites, as well as by the fact that in no case do buoyant densities reach the high values found in the GC-richest components of DNAs from warm-blooded vertebrates.In the case of fish genomes, which were more extensively studied, different orders were generally characterized by modal buoyant densities that were different in average values as well as in their ranges. In contrast, different families within any given order were more often characterized by narrow ranges of modal buoyant densities, and no difference in modal buoyant density was found within any single genus (except for the genusAphyosemion, which should be split into several genera).The compositional differences that were found among species belonging to different orders and to different families within the same order are indicative of compositional transitions, which were shown to be essentially due to directional base substitutions. These transitions were found to be independent of geological time. Moreover, the rates of directional base substitutions were found to be very variable and to reach, in some cases, extremely high values, that were even higher than those of silent substitutions in primates. The taxonomic and evolutionary implications of these findings are discussed.  相似文献   

2.
Buoyant densities of DNA of mammals   总被引:9,自引:0,他引:9  
One characteristic of DNA, CsCl buoyant density peak values, was determined for DNA samples isolated from 93 species belonging to 11 orders of mammals. The CsCl buoyant density values varied over a very narrow range, 1.696–1.701 g/cm3. Satellite DNAs were found in a number of species. The function and origin of these satellite DNAs are not known.This work was supported by grant DRG-269 from the Damon Runyon Memorial Fund for Cancer Research, Inc., and GB-6657 from the National Science Foundation.  相似文献   

3.
An analysis of the bovine genome by Cs2SO4-Ag density gradient centrifugation   总被引:22,自引:0,他引:22  
Calf DNA preparations having molecular weights of 5 to 7 × 106 have been fractionated by preparative Cs2SO4—Ag+ density gradient centrifugation into a number of components. These may be divided into three groups: (1) the main DNA component (1.697 g/cm3; all densities quoted are those determined in CsCl density gradients), the 1.704 and 1.709 g/cm3 components form about 50, 25 and 10% of the genome, respectively; they are characterized by having symmetrical CsCl bands and melting curves, both of which have standard deviations close to those of bacterial DNAs of comparable molecular weight, and by their G + C contents being equal to 39, 48 and 54%, respectively; after heat-denaturation and reannealing, their buoyant densities in CsCl are greater than native DNA by 12, 10 and 3 mg/cm3, respectively. (2) The 1.705, 1.710, 1.714 and 1.723 g/cm3 components represent 4, 1.5, 7 and 1.5% of the DNA, respectively, and exhibit the properties of “satellite” DNAs; their CsCl bands and melting curves have standard deviations lower than those of bacterial DNAs; after heat-denaturation and reannealing, their buoyant densities are identical to native DNA, except for the 1.705 g/cm3 component, which remains heavier by 5 mg/cm3; in alkaline CsCl, only the 1.714 g/cm3 component shows a strand separation. (3) A number of minor components, forming 1% of the DNA, have been recognized, but they have not been investigated in detail; two of them (1.719 and 1.699 g/cm3) might correspond to ribosomal cistrons and mitochondrial DNA, respectively.  相似文献   

4.
Bucciarelli G  Bernardi G  Bernardi G 《Gene》2002,295(2):153-162
The goal of this study was to provide a comprehensive view of the compositional characteristics of fish genomes. We therefore expanded the number of fish species that we had explored so far in their DNAs by analytical ultracentrifugation in CsCl density gradient from 122 to 201. This study included representatives from three out of nine orders of Elasmobranchs (sharks and rays), both orders of dipnoan lungfishes, and both orders of chondrosteans (sturgeons and bichirs). We also studied 19 out of 38 teleostean orders, which represent all but four (minor) superorders of the subdivision Teleostei, a group comprising about 23,600 species (96% of all extant fishes). This leaves for further studies two subclasses, Holocephali (chimaeras), and Coelacanthimorpha (gombessas). In spite of this substantial increase in the number of species and orders analysed, all average properties (the modal buoyant density, rho(0), the average buoyant density, , the CsCl profile asymmetry, A, and the compositional heterogeneity, H), and all their ranges were unchanged compared to a previous study [J. Mol. Evol. 31 (1990) 265]. This suggests that, in all likelihood, the properties reported in the present paper can be considered as generally valid for all fish genomes.  相似文献   

5.
Nuclear DNA from liver cells of the kangaroo rat species Dipodomysordii was fractionated and characterized with the aid of buoyant density gradients in neutral and alkaline CsCl and in Ag+-Cs2SO4. More than one-half of the DNA was present in three density satellites, a greater proportion than in any other species yet reported; the purified satellite DNAs were denser than principal DNA. All satellite fractions revealed sharp isopycnic bands and narrow denaturation profiles. Two had identical buoyant densities but differed substantially in Tm, base composition, and reassociation kinetics. In alkaline CsCl all three satellites, as well as a shoulder of intermediate repetitive DNA on the heavy side of the principal band, revealed unique strand densities. The most highly repetitive satellite was unusually rich in (G + C) and contained 6.7% of 5-methylcytosine. A survey of internal organs and spermatozoa of an adult male revealed no significant differences in distribution of the satellites among tissues.  相似文献   

6.
Satellite DNA associated with heterochromatin in Rhynchosciara   总被引:8,自引:0,他引:8  
The DNA of Rhynchosciara hollaenderi was examined using isopycnic centrifugation in neutral CsCl. Two low density minor bands (collectively termed satellite DNA) were detected in addition to the main band DNA. Main band DNA has a buoyant density of 1.695 g/cm3. The larger of the two minor bands has a buoyant density of 1.680 g/cm3 while the smaller of the two minor bands has a buoyant density of about 1.675 g/cm3. Thermal denaturation studies have confirmed the presence of the two minor classes of DNA.—The satellite and main band DNAs were isolated in relatively pure form and were transcribed in vitro using DNA-dependent RNA polymerase from Escherichia coli. Annealing of the two complementary RNAs (cRNAs) with main band and satellite DNA was examined using filter hybridization techniques.—The chromosomal distribution of the satellite DNA was determined by in situ molecular hybridization of satellite-cRNA with Rhynchosciara salivary gland chromosomes. Satellite-cRNA hybridized with the centromeric heterochromatin of each of the four chromosomes (A, B, C, and X) and with certain densely staining bands in the telomere regions of the A and C chromosomes. Main band-cRNA annealed with many loci scattered throughout the chromosomes including areas containing satellite DNA.  相似文献   

7.
Hughes S  Clay O  Bernardi G 《Gene》2002,295(2):323-329
Sauropsids form a complex group of vertebrates including squamates (lizards and snakes), turtles, crocodiles, sphenodon and birds (which are often considered as a separate class). Although avian genomes have been relatively well studied, the genomes of the other groups have remained only sparsely characterized. Moreover, the nuclear sequences available in databanks are still very limited. In the present study, we have analysed the compositional patterns, i.e. the GC (molar fraction of guanine and cytosine in DNA) distributions, of 31 reptilian (particularly snake) genomes by analytical ultracentrifugation of DNAs in CsCl gradients. The profiles were characterized by their modal buoyant density rho(o), mean buoyant density < rho>, asymmetry < rho>- rho(o), and heterogeneity H. The modal buoyant density distribution of reptilian DNAs clearly distinguishes two groups. The snakes fall in the same range of modal densities as most mammals, whereas crocodiles, turtles and lizards show higher values (>1.700 g/cm(3)). As far as the more important compositional properties of asymmetry and heterogeneity are concerned, previous studies showed that amphibians and fishes share relatively low values, whereas birds and mammals are characterized by highly heterogeneous and asymmetric patterns (with the exception of Muridae, which have a lower heterogeneity). The present results show that the snake genomes cover a broad range of asymmetry and heterogeneity values, whereas the genomes of crocodiles and turtles cover a narrow range that is intermediate between those of fishes/amphibians and those of mammals/birds.  相似文献   

8.
Fortes GG  Bouza C  Martínez P  Sánchez L 《Genetica》2007,129(3):281-289
To review the general consideration about the different compositional structure of warm and cold-blooded vertebrates genomes, we used of the increasing number of genetic sequences, including coding (exons) and non-coding (introns) regions, that have been deposited on the databases throughout last years. The nucleotide distributions of the third codon positions (GC3) have been analyzed in 1510 coding sequences (CDS) of fish, 1414 CDS of amphibians and 320 CDS of reptiles. Also, the relationship between GC content of 74, 56 and 25 CDS of fish, amphibians and reptiles, respectively and that of their corresponding introns (GCI) have been considerated. In accordance with recent data, sequence analysis showed the presence of very GC3-rich CDS in these poikilotherm vertebrates. However, very high diversity in compositional patterns among different orders of fish, amphibians and reptiles was found. Significant positive correlations between GC3 and GCI was also confirmed for the genes analyzed. Nevertheless, introns resulted to be poorer in GC than their corresponding CDS, this difference being larger than in human genome. Because the limited number of available sequences including exons and introns we must be cautious about the results derived from them. However, the indicious of higher GC richness of coding sequences than of their corresponding introns could aid to understand the discrepancy of sequence analysis with the ultracentrifugation studies in cold-blooded vertebrates that did not predict the existence of GC-rich isochores.  相似文献   

9.
The equilibrium banding behavior of high molecular weight DNA has been examined after centrifugation in CsCl gradients on angle rotors in the Spinco preparative ultracentrifuge. DNAs of base composition ranging from 30% GC to 71% GC, from a variety of species, band in close agreement to a calculated curve relating density and radial distance in angle preparative gradients, but only if the DNA is sheared prior to centrifugation. Unsheared DNA, after bottom tube puneture and drop collection by conventional methods, displays a characteristic unsymmetrical profile and the fraction with the largest concentration of DNA appears in a much lower fraction number than expected. This artifact leads to misealculation of buoyant densities from preparative gradients and interferes with fractionation of DNA, as for instance in separating satellite bands. Collection of the gradient from the top reverses the direction of the profile asymmetry and shearing eliminates it entirely.  相似文献   

10.
The compositional distributions of high molecular weight DNA fragments from 20 species belonging to 9 out of the 17 eutherian orders were investigated by analytical CsCl density gradient centrifugation and by preparative fractionation in Cs2SO4/BAMD density gradients followed by analysis of the fractions in CsCl. These compositional distributions reflect those of the isochores making up the corresponding genomes. A “general distribution” was found in species belonging to eight mammalian orders. A “myomorph distribution” was found in Myomorpha, but not in the other rodent infraorders Sciuromorpha and Histricomorpha, which share the general distribution. Two other distributions were found in a megachiropteran (but not in microchiropteran, which, again, shares the general distribution) and in pangolin (a species from the only genus of the order Pholidota), respectively. The main difference between the general distribution and all other distributions is that the former contains sizable amounts (6–10%) of GC-rich isochores (detected as DNA fragments equal to, or higher than, 1.710 g/cm3 in modal buoyant density), which are scarce, or absent, in the other distributions. This difference is remarkable because gene concentrations in mammalian genomes are paralleled by GC levels, the highest gene concentrations being present in the GC-richest isochores. The compositional distributions of mammalian genomes reported here shed light on mammalian phylogeny. Indeed, all orders investigated, with the exception of Pholidota, seem to share a common ancestor. The compositional patterns of the megachiropteran and of Myomorpha may be derived from the general pattern or have independent origins.  相似文献   

11.
Two AT-rich satellite DNAs are present in the genome of Glyptotendipes barbipes. The two satellites have densities of 1.680 g/cm3 (=21% GC) and of 1.673 g/cm3 (=13% GC) in neutral CsCl-density gradients. The main band DNA has a density of 1.691 g/cm3 (=32% GC). This value is in agreement with the 33% GC-content of G. barbipes DNA calculated from thermal denaturation (TM=83° C). — In brain DNA as well as in salivary gland DNA the two satellite sequences together comprise 12–15% of the total G. barbipes DNA. Comparisons of the density profiles of DNA extracted from polytene and non-polytene larval tissue gave no hints for underreplication of the satellite DNAs during polytenization. — The two satellite DNAs have been isolated from total DNA by Hoechst 33258-CsCl density centrifugation and then localized in the polytene salivary gland chromosomes by in situ hybridization. Both satellite sequences hybridize to all heterochromatic centromere bands of all four chromosomes of G. barbipes. Satellite I (1.673 g/cm3) hybridizes mainly with the middle of the heterochromatin, satellite II (1.680 g/cm3) hybridizes with two bands at the margin of the heterochromatin. In situ hybridization with polytene chromosomes of Chironomus thummi revealed the presence of G. barbipes satellite sequences also in the Ch. thummi genome at various locations, mainly the centromere regions.  相似文献   

12.
Sheep and goats possess closely similar satellite DNAs which seem to be concentrated in the heterochromatin and the nucleolus. In both species, the major and minor satellites have buoyant densities of 1.711 g·cm –3 and 1.718 g·cm –3 and represent 12% and 2.5%, respectively, of the total DNA. Major satellite concentration in the combined heterochromatin and nucleolar fraction was 19%, and in the pure nucleolar fraction it was 51%. Most hitherto reported satellite DNAs have varied markedly in density and concentration from species to species, even within the same genus. The close similarity of the sheep and goat satellite is discussed in the light of what is known about the evolutionary relationship between the sheep and the goat and in the light of various hypotheses concerning the possible functions of satellite DNA.  相似文献   

13.
Summary As in wild-type Nicotiana tabacum L., two satellite DNAs having densities of 1.700 and 1.705 g cm–3 in CsCl were identified in the organelle fraction of homogenates made from variegated leaves of a cytoplasmic mutant of N. tabacum. As the proportion of white to green tissue increased a great reduction in the 1.700 chloroplast DNA occurred correlated with a concomitant reduction in the total number of defective and normal chloroplasts per cell. At the same time, there was an absolute increase in the 1.705 satellite DNA. Separation of the two satellite DNAs was achieved by one cycle of purification on NaI gradients. When the 1.700 chloroplast DNAs from white and from green tissue of variegated leaves were compared, identical properties were found by the conventional buoyant density, T m and renaturation kinetics measurements. However, using a specially constructed difference melting system, the 1.700 DNA from defective chloroplasts was shown to have an approximately 1% higher GC composition than the DNA from normal chloroplasts. Also, by renaturation of a mixture of alkali denatured normal and defective chloroplast DNAs and subsequent spreading in formamide for electron microscopy, internal regions of mismatching were observed. The nonhomologous region corresponded to about 500–1000 base pairs. No differences in composition of the 1.705 satellite DNA derived from white or green tissues were detected either by difference melting or formation of heteroduplexes.  相似文献   

14.
M. Steinemann 《Chromosoma》1978,66(2):127-139
The DNAs of five Chironomus species, C. plumosus, C. nuditarsis, C. thummi thummi, C. melanotus, and Camptochironomus pallidivittatus, were investigated in analytical neutral isopycnic CsCl density gradients. DNA was isolated both from larval brains (diploid-DNA) and salivary glands (polytene-DNA). The buoyant densities of mainband DNAs were 1.692 g/cm3, with the exception of C. melanotus whose mainband had a density of 1.693 g/cm3. The densities correspond to a calculated GC content of 33% and 34% respectively. Only in C. melanotus was the DNA clearly resolved into mainband DNA and two distinct satellite shoulders: Satellite I, with a buoyant density of 1.684 g/cm3 (25% GC, calculated) and satellite II of 1.679 g/cm3 (19% GC, calculated). The two satellites comprise 15% of the total DNA in the diploid-DNA and they also occur in the polytene-DNA, where they make up 11%. The results are discussed in the general context of under- and overreplication in polyploid and polytene cells.  相似文献   

15.
Calf thymus DNA containing satellite components of various densities was used as a model to study the effect of netropsin on the density of DNA in a CsCl gradient. The binding of netropsin resulted in a decrease in density which depended upon the quantity of netropsin added and on the average composition of the DNA. Differences in density of DNA components were higher in CsCl - netropsin gradients than in simple CsCl gradients. By use of netropsin a main band and four satellite bands could be differentiated in calf thymus DNA. Satellite DNA's were isolated using preparative CsCl - netropsin gradient centrifugation and were characterised by density and homogeneity in native and in reassociated state. Two of the satellite components, with densities of 1.722 and 1.714 g/cm3, are probably of homogenous sequence, the other two components of densities 1.709 and 1.705 g/cm3 appear to be heterogeneous.  相似文献   

16.
Location of Satellite and Homogeneous DNA Sequences on Human Chromosomes   总被引:15,自引:0,他引:15  
HUMAN DNA1,2 contains at least two satellite fractions—satellite I (0.5% of the genome) which bands at a density of 1.687 g/cm3 in neutral CsCl and satellite II (2% of the genome) which bands at 1.693 g/cm3. The main band DNA has an average buoyant density between 1.670 and 1.720 g/cm3 and a light shoulder (constituting 15% of the genome) with a buoyant density of 1.696 g/cm3, referred to as homogeneous mainband. Satellite DNA has been observed in many higher organisms3, usually with an unknown function, notable exceptions being cistrons coding for ribosomal RNA4 and the DNA coding for histone messenger RNA5. To investigate possible functions of human repetitive DNA we have studied the annealing of complementary RNA fractions to chromosomes using in situ hybridization6,7. We describe here preliminary observations using human satellite II and homogeneous mainband fractions.  相似文献   

17.
When haploid yeast strains containing mitochondrial DNAs (mtDNAs) of different buoyant densities are mated, the resulting zygotes contain a mixed population of mitochondria and mitochondrial DNAs. During vegetative growth of diploid cells formed from such a cross between a petite strain with mtDNA of density 1.677 g cm?3 and a respiratory competent strain with mtDNA of density 1.684 g cm?3, mtDNAs with intermediate buoyant densities are obtained. Virtually all newly synthesized mtDNA in diploid ρ? progeny has the intermediate buoyant density. Therefore, within 2 generations of growth of the diploid cells, the intermediate buoyant density species predominate. In crosses between a respiratory competent strain and other petite strains with different values of genetic suppressiveness, it was found that the amount of recombination yielding mtDNAs of intermediate buoyant densities roughly parallels the degree of suppressiveness. Individual clones of respiratory deficient cells from such crosses were also isolated to confirm that stable mtDNAs with intermediate buoyant densities were obtained. Thus, it is apparent that some form of recombination takes place within the mtDNAs of yeast cells that results in stable mtDNA species.  相似文献   

18.
Murine type B particles were separated from type C (Rauscher leukemia virus) by means of gentle (low-increment rate) density gradients. The best separation was obtained when the density ranged from 1.13 to 1.20 g/cm3 when sucrose was used and from 1.12 to 1.28 g/cm3 with CsCl. The buoyant densities of the B and C particle bands in sucrose were 1.18 and 1.16 g/cm3, respectively. The CsCl gradient gave a better separation with the B particles banding at a density of 1.20 g/cm3 and with the C particle density little different from its value in sucrose.  相似文献   

19.
When partially purified Eastern equine encephalitis (EEE) virus was centrifuged to equilibrium in CsCl, three virus specific bands were observed. A hemagglutinin was detected at a buoyant density of 1.18 g/cm3. Infectious EEE virus banded in two positions; most of the virus banded at 1.20 g/cm3 and a lesser amount banded at 1.22 to 1.23 g/cm3. Analysis of radioactive profiles of CsCl-fractionated EEE virus labeled with either 32PO4 or 3H-uridine suggested that the hemagglutinin was stripped from the intact EEE virion. The viral origin of the hemagglutinin was verified by inhibition with specific antiserum. Attempts to differentiate between infectious EEE virus of the different buoyant densities showed that the denser particle was neither a virus contaminant nor a density mutant. No evidence was obtained to indicate that the denser particle was an immature form of EEE virus. The two infectious EEE species obtained after CsCl fractionation were indistinguishable antigenically. Furthermore, unfractionated as well as CsCl-fractionated EEE virus sedimented at about 260S in sucrose gradients. These results together with the results of rebanding experiments suggested that the denser EEE species (1.23 g/cm3) results from a salt (CsCl)-induced alteration or breakdown of the EEE virion (1.20 g/cm3), and that it arises as the hemagglutinin is stripped from the surface of the EEE virion.  相似文献   

20.
Amoeba proteus synthesizes DNA in G2 phase of the cell cycle upon feeding after starvation. The characteristics of the DNA synthesized in G2 have been studied by microscope photometry of individual Feulgen-stained nuclei and by buoyant density centrifugation of nuclear DNA in CsCl. Amoeba nuclei were found to contain 42.8 pg of DNA. This DNA bands in CsCl at a density of 1.693 g/cm3 with a satellite at 1.714 g/cm3 which makes up 24% of nuclear DNA. DNA from whole cells has an additional non-nuclear satellite at 1.726 g/cm3. When cells are starved and re-fed with food labeled with [3H]thymidine, the DNA synthesized is predominantly the 1.714 satellite. The amount of DNA synthesized in G2 is small since there is no measurable difference in Feulgen dye binding to nuclei of starved vs starved and re-fed cells. The data suggest that refeeding induces a resumption of late S phase DNA synthesis, or the preferential synthesis of specific DNA sequences such as rRNA genes.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号